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1.
Mikrochim Acta ; 187(3): 163, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32052190

RESUMO

An electrochemical aptasensor for thrombin is introduced that makes use of a nanohybrid composed of silver nanoparticles and graphite-like carbon nitride (Ag-g-C3N4). The material has a large surface and good biocompatibility. AgNPs are modified directly on the surface of g-C3N4 via chemical reduction. A glass carbon electrode (GCE) modified with Ag-g-C3N4 can immobilize a large number of amino-terminated thrombin binding aptamers (NH2-TBA) through strong Ag-N bonds. The electrochemical impedance signal of the aptasensor increases in the presence of thrombin. Under the optimal conditions and by using [Fe(CN)6]3-/4- as an electrochemical probe, the aptasensor shows a wide linear range of 100 fM - 20 nM with a lower detection limit of 38 fM. The method was applied to the determination of thrombin in spiked human plasma and the recoveries fluctuated from 97.2% to 103%. Graphical abstractSchematic representation of an electrochemical aptasensor using graphite-like carbon nitride (C3N4) modified with silver nanoparticles as electrode substrate for thrombin (TB) detection.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 231: 118117, 2020 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-32066077

RESUMO

The development of new structures allows two-photon coherent anti-Stokes Raman scattering (TPCARS) to be strongly enhanced by multiple surface plasmon resonances (MSPRs). In this paper, plasmonic structure consisting of two Ag nanorods is designed and the enhancement of TPCARS is investigated. By properly selecting designing structure parameters, strong MSPRs peaks at 1020 nm and 505 nm are obtained, which can enhance the TPCARS signal based on the frequency match of the fundamental frequency and frequency doubling. The enhancement factor of TPCARS can reach as high as 3.66 × 1028 with significant electric field enhancements under appropriate selection of system parameters. Furthermore, the two-photon process can be controlled at different optical frequencies by changing the geometric parameters of Ag nanorods. The new scheme advanced in this work can help to achieve single molecule level of CARS, and may have a potential to increase the intensity and resolution of nonlinear optical imaging.

3.
Thyroid ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32031055

RESUMO

Background: Sporadic medullary thyroid carcinoma (MTC) is a relatively uncommon neuroendocrine malignancy, and the molecular tumorigenesis of its sporadic type (sMTC) is not clear enough. Here we performed a study focusing on the genomic and transcriptomic characterization of sMTC. Methods: Twenty-nine sMTC patients were included. Whole-exome sequencing (WES) was carried out in 18 patients with both tumor samples and matched noncancerous tissues. Whole transcriptome sequencing (RNA-Seq) was performed in all 29 tumors. WES, RNA-Seq and copy number alteration (CNA) data were analyzed. Cell Counting Kit-8 (CCK-8) assay was used to evaluate cell proliferation. Results: In the somatic setting, RET was the only recurrently cancer-related mutated gene (5/18, 27.8%). In the germline setting, FAT1 and FAT4, two members of the FAT gene family, were identified as the two most common mutated genes. Meanwhile, CNA analysis found that FAT1 and FAT4, both located at chromosome 4q, were also two of the most commonly affected genes by somatic chromosomal deletions (4/18, 22.2%). Using TT and MZ-CRC-1 cell lines, CCK-8 assay showed that FAT1 and FAT4 knockdown could both promote MTC cell proliferation. Based on the gene expression profile, patients were clustered into two molecular subtypes: the mesenchymal-like subtype was characterized by epithelial-mesenchymal transition, while the proliferative-like subtype was associated with enrichment of cell cycle pathways. Most events of structural recurrence (80%) occurred in the proliferative-like subtype. Conclusion: In addition to RET, our research also shows there may be high-frequency FAT1/FAT4 genomic alterations in sMTC. Two molecular subtypes of sMTC with distinct biological behaviors are also identified. However, our results need to be validated by larger samples and more comprehensive experiments in the future, especially for the frequency and function of FAT1/FAT4 germline variants.

4.
J Virol ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32051271

RESUMO

Given that the Ebola virus (EBOV) infects a wide array of organs and cells yet displays a relative lack of neurotropism, we asked whether a chimeric vesicular stomatitis virus (VSV) expressing the EBOV glycoprotein (GP) might selectively target brain tumors. The mucin-like domain (MLD) of the EBOV GP may enhance virus immune system evasion. Here we compare chimeric VSVs in which EBOV GP replaces the VSV glycoprotein, thereby reducing the neurotoxicity associated with wildtype VSV. A chimeric VSV expressing the full-length EBOV GP (VSV-EBOV) containing the MLD was substantially more effective and safer than a parallel construct with an EBOV GP lacking the MLD (VSV-EBOVΔMLD). One-step growth, RT-qPCR, and Western blot assessment showed VSV-EBOVΔMLD produced substantially more progeny faster than VSV-EBOV. Using immunodeficient SCID mice, we focused on targeting human brain tumors with these VSV-EBOVs. Similar to our previous report using an attenuated VSV-EBOV with no MLD that expressed GFP (VSV-EBOVΔMLD-GFP), VSV-EBOVΔMLD without GFP targeted glioma, but yielded only a modest extension of survival. In contrast, VSV-EBOV containing the MLD showed substantially better targeting and elimination of brain tumors after intravenous delivery, and increased the survival of brain tumor-bearing mice. Despite the apparent destruction of most tumor cells by VSV-EBOVΔMLD, the virus remained active within the SCID mouse brain and showed widespread infection of normal brain cells. In contrast, VSV-EBOV eliminated the tumors, and showed relatively little infection of normal brain cells. Parallel experiments with direct intracranial virus infection generated similar results. Neither VSV-EBOV nor VSV-EBOVΔMLD showed substantive infection of the brains of normal immunocompetent mice.Importance The Ebola virus glycoprotein contains a mucin-like domain which may play a role in immune evasion. Chimeric vesicular stomatitis viruses with the EBOV glycoprotein substituted for the VSV glycoprotein show greater safety and efficacy in targeting brain tumors in immunodeficient mice when the MLD is expressed within the EBOV glycoprotein compared with EBOV lacking the mucin-like domain.

5.
J Ethnopharmacol ; : 112638, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32007633

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Laryngeal carcinoma (LC) is one of the most common malignant head and neck cancers with high incidence and mortality rates. Erchen decoction plus Huiyanzhuyu decoction (EHD) is commonly used for treating LC patients and produces beneficial results. However, the mechanisms underlying the effects of EHD remain unclear. AIM OF THE STUDY: The present study aimed to analyse the anticancer effects of EHD on the LC cell cycle, apoptosis, migration and invasion in vitro and to explore the underlying biological mechanisms. MATERIALS AND METHODS: TU212 and Hep-2 cells were used. The antitumour effects of EHD were detected by CCK8, microscopy, flow cytometry, EdU incorporation, Hoechst 33342 staining, wound-healing, and transwell assays to assess viability, morphology, apoptosis, cell cycle, migration and invasion, respectively. Furthermore, STAT3 and related proteins were evaluated in laryngeal squamous cell carcinoma (LSCC) cells by Western blot (WB) analysis. RESULTS: EHD treatment significantly decreased STAT3 and p-STAT3 protein expression levels in LSCC cells. EHD blocked the cell cycle at the G0/G1 phase and induced LSCC apoptosis. Moreover, the viability, migration, and invasion of LSCC cells were markedly inhibited by EHD. In addition, the expression of the cell cycle-related proteins cyclin D1 and cyclin B1 was downregulated in LSCC cells, but P27 expression was increased after EHD treatment. Regarding apoptosis-related proteins, EHD also reduced Bcl-2 expression but upregulated Bax and caspase-3 expression in LSCC cells. In the migration- and invasion-related protein analyses, EHD downregulated MMP-9 expression and upregulated E-cadherin expression. CONCLUSIONS: These results suggest that EHD has an anticancer effect in LSCC. EHD treatment induces apoptosis and inhibits the cell cycle, migration and invasion of LSCC cells, but further work is warranted to address the mechanisms.

6.
J Perianesth Nurs ; 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31973960

RESUMO

PURPOSE: The study was designed to evaluate the effect of an individualized fasting program on fasting time and comfort in infants and young children during the perioperative period. DESIGN: A quasiexperimental design was used. METHODS: The study included 675 children (intervention = 353, control = 322). Data collection tools included Characteristics of Children Form and the Infant Hunger Rating Scale. The fasting program included individualized fasting education and fasting in batches. On the day of the operation, clear liquids were fed 2 hours before surgery and refed after the patient woke after surgery. FINDINGS: The duration of perioperative fasting and the time to refeeding were shorter, the hunger scores were lower in the intervention group than those in the control group (P < .05). There was no difference in the incidence of vomiting between the two groups (P > .05), and no coughing and bloating occurred. CONCLUSIONS: The fasting program for infants and young children can shorten the duration of fasting and can reduce the degree of hunger. This program is safe and feasible.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31935950

RESUMO

Telemedicine is an innovative approach that helps alleviate the health disparity in developing countries and improve health service accessibility, affordability, and quality. Few studies have focused on the social and organizational issues involved in telemedicine, despite in-depth studies of and significant improvements in these technologies. This paper used evolutionary game theory to analyze behavioral strategies and their dynamic evolution in the implementation and operation of telemedicine. Further, numerical simulation was carried out to develop management strategies for promoting telemedicine as a new way of delivering health services. The results showed that: (1) When the benefits are greater than the costs, the higher medical institutions (HMIs), primary medical institutions (PMIs), and patients positively promote telemedicine with benign interactions; (2) when the costs are greater than the benefits, the stability strategy of HMIs, PMIs, and patients is, respectively, 'no efforts', 'no efforts', and 'non-acceptance'; and (3) promotion of telemedicine is influenced by the initial probability of the 'HMI efforts', 'PMI efforts', and 'patients' acceptance' strategy chosen by the three stakeholders, telemedicine costs, and the reimbursement ratio of such costs. Based on theoretical analysis, in order to verify the theoretical model, this paper introduces the case study of a telemedicine system integrated with health resources at provincial, municipal, county, and township level in Guizhou. The findings of the case study were consistent with the theoretical analysis. Therefore, the central Chinese government and local governments should pay attention to the running cost of MIs and provide financial support when the costs are greater than the benefits. At the same time, the government should raise awareness of telemedicine and increase participation by all three stakeholders. Lastly, in order to promote telemedicine effectively, it is recommended that telemedicine services are incorporated within the scope of medical insurance and the optimal reimbursement ratio is used.

8.
J Am Med Dir Assoc ; 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31932139

RESUMO

OBJECTIVES: Clostridioides difficile infection is a major source of morbidity and mortality among frail older adults, especially those in nursing homes (NHs). Safety reports have signaled that bisphosphonate use may be a contributing cause. We therefore evaluated the risk of C difficile hospitalization associated with oral bisphosphonate use in the NH. DESIGN: Observational, retrospective new-user cohort study. SETTING: The cohort included US NH residents aged ≥65 years who became a long-stay resident (>100 days in the NH) between January 1, 2008 and December 31, 2009. METHODS: We conducted a study of NH residents using linked Medicare claims and Minimum Data Set records. Residents were new users of an oral bisphosphonate 1:1 matched to new calcitonin users ("active" comparator) on propensity scores controlling for more than 100 covariates. The outcome was risk of hospitalization for C difficile infection in a Cox proportional hazards model adjusted for previous antibiotic and proton pump inhibitor use. RESULTS: Our final analytical cohort included 17,753 bisphosphonate and 5348 calcitonin users. In the matched cohort, 84/5209 (1.6%) vs 71/5209 (1.4%) C difficile-related hospitalizations occurred in bisphosphonate and calcitonin users, respectively. We observed no significant difference in the risk of hospitalization among bisphosphonate users (hazard ratio: 1.11, 95% confidence interval: 0.80-1.51). Antibiotic and proton pump inhibitor exposure before and after osteoporosis treatment was also similar between bisphosphonate and calcitonin users. CONCLUSIONS AND IMPLICATIONS: C difficile infection should not be a consideration when prescribing bisphosphonates to frail older adults given the lack of a significant association.

9.
Anal Chim Acta ; 1098: 164-169, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31948580

RESUMO

Detecting molecular biomarkers in high sensitivity plays an important role in the diagnosis of various diseases at the early stage. Here, by combining the target-induced polymerization nicking reaction (TIPNR) with the loop-mediated isothermal amplification (LAMP), we describe an ultrasensitive and label-free aptamer-based sensing method for detecting low levels of proteins in human serum by using thrombin as the model target analyte. The target thrombin binds and causes spontaneous assembly of two distinct aptamer probes to form the templates for the polymerization nicking reaction recycling amplification to produce many forward inner primer sequences. Subsequently, downstream LAMP reactions are initiated by these sequences for the generation of tremendous DNA hairpins with various lengths via automated cyclic strand displacement reactions. The SYBR Green I organic dye further binds the many hairpins to show drastically amplified fluorescence for ultrasensitive detection of thrombin down to 3.6 fM in the linear range from 0.01 pM to 10 nM. Such a sensing method based on aptamers has high discrimination capability for the target molecules against other non-specific proteins and is applicable for diluted serum samples. With the successful demonstration of the substantial signal amplification ability and simplicity feature of this assay approach, highly sensitive and convenient detection of other disease biomarkers with this method can be envisioned in the near future.

10.
PLoS One ; 15(1): e0227617, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31914168

RESUMO

A Yunnan branched-spike (Ynbs) barley mutant is useful for study of the genetic mechanisms underlying variation in barley spike architecture. In the current study, a mutant (Ynbs-1), a recombinant inbred line (RIL-1), and a cultivar (BDM-8) were used as parents to develop populations. Ynbs-1 exhibits typical branched spike, whereas the others exhibit six-row spike. Genetic analysis on their F1, F2 and F3 populations showed that one recessive gene is responsible for the branched spike trait. SLAF marker generated from specific locus amplified fragment sequencing (SLAF-seq) was used to genotype the populations. A high-density genetic map of barley was constructed using 14,348 SLAF markers, which covered all 7 chromosomes at 1,347.44 cM in length with an average marker density of 0.09 cM between adjacent markers. Linkage analysis of the branched-spike trait using the genetic map indicated that branched spike trait in the Ynbs-1 is controlled by single locus on chromosome 2H at the interval between 65.00 and 65.47 cM that is flanked by Marker310119 and Marker2679451. Several candidate genes that may be responsible for barley multiple-spikelet degeneration, single-floret spikelet increase and seed set rate decrease were identified in the region. The high-density genetic map and the gene locus revealed in this study provide valuable information for elucidating the genetic mechanism of spike branching in barley.

11.
J Mol Model ; 26(2): 34, 2020 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-31982972

RESUMO

A series of dyes are designed by adding the different electron-donating (-CH3, -NH2, -OH) and electron-withdrawing groups (-Br, -Cl, -NO2) to the different ancillary ligands in the alkynylrhenium(I) tricarbonyl diimine complexes [Re(CO)3(N^N){C≡C-C6H4-CH=C(CN)(COOH)}], where N^N = 1,10-phenanthroline (phen)(1) and then investigated the sensitization properties of dyes linked to the TiO2(101) surface. Density functional theory (DFT) and time-dependent density functional theory (TD-DFT) were used to study the electronic structure, frontier molecular orbitals, and absorption spectral properties. The effect of group-substitution on sensitization properties is obvious. When the dye molecules are combined with TiO2(101) surface, not only the absorptions of some sensitizers containing -CH3 or -OH groups have red shift but also the electrons can be directly injected into the TiO2 conduction band from the dye molecules compared with the parent molecular 1. The results indicate that the designed dyes containing electron-donating groups have smaller energy gaps, better light-harvesting efficiency, sufficient driving force, and higher charge transfer efficiency as appropriate dye sensitizers. We hope it can provide valuable hints so that we can design more efficient dye sensitizers in DSSCs.

12.
Arch Gynecol Obstet ; 2020 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-31903498

RESUMO

PURPOSE: Recent studies have demonstrated the differential expression of micro(mi)RNAs in endometriosis. Previously, we reported the low expression of miR-141 in patients with this disease. Epithelial-to-mesenchymal transition (EMT) and the transforming growth factor-beta1 (TGF-ß1)-induced SMAD2 signalling pathway are central to tumour proliferation and invasion. However, the role of miR-141 in regulating the TGF-ß1/SMAD2 signalling pathway and the associated EMT to be elucidated. METHODS: The levels of TGF-ß1/SMAD2 signalling and EMT markers expression in eutopic and ectopic endometria of endometriosis were determined by immunohistochemistry and western blot analyses. MiR-141 expression was analysed by quantitative reverse-transcription polymerase chain reaction. Cellular invasion and proliferation were determined by transwell and CCK-8 assays, respectively. Functional assay of miR-141 was performed using plasmid and shRNA transfection methods. RESULT: The presence of miR-141, EMT, and TGF-ß1/SMAD2 signalling markers were detected in eutopic and ectopic endometria of endometriosis. TGF-ß1-induced EMT in Ishikawa (ISK) cells by activating the SMAD2 signalling pathway, whereas miR-141 inhibited the TGF-ß1-induced EMT, proliferation and invasion abilities of these cells. CONCLUSION: These data identify miR-141 as a novel driver of EMT in endometriosis, implicates the link between miR-141 and TGF-ß1/SMAD2 signalling pathway in the context of endometriosis, and underscore the role of EMT in the development of endometriosis.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31907179

RESUMO

Blood concentration of isoniazid (INH) is evidently affected by N-acetyltransferase-2 (NAT2) polymorphism, an enzyme that is primarily responsible for the tri-modal (i.e. fast, intermediate and slow) INH elimination. The pharmacokinetic (PK) variability, driven largely by NAT2 activity, creates a challenge for the deployment of uniform INH dosage in tuberculosis (TB) patients. Although, acetylator-specific INH dosing has long been suggested, their well-recognized dosages remain elusive. In this study, 175 blood samples were collected from 89 pulmonary TB patients within 0.5-6 h after morning administration. According to their NAT2 genotypes, 32 (36.0%), 38 (42.7%) and 19 (21.3%) were fast, intermediate and slow acetylators, respectively. The plasma INH concentration was detected by liquid chromatography-tandem mass spectrometry. Population pharmacokinetic (PPK) analysis was conducted using NONMEM and R software. A two-compartment model with first-order absorption and elimination well described the PK parameters of isoniazid. Body weight and acetylator-status significantly affected the INH clearance rate. The dosage simulation targeting three indicators, including the well-recognized efficacy-safety Cmax serum concentration (3-6 µg/mL), the reported area under the concentration-time curve to infinity (AUC(0,∞)) (≥ 10.52µg/ml per hour), and the 2-h INH serum concentrations (≥ 2.19 µg/ml) that associated with the strongest early bactericidal activity. The optimal dosages, targeting different indicators, varied between 700-900 mg/day, 500-600 mg/day and 300 mg/day for rapid, intermediate and slow acetylators, respectively. Furthermore, a PPK model for isoniazid among Chinese tuberculosis patients was established for the first time which suggested an approximated 800 mg/day, 500 mg/day or 300 mg/day dose for fast, intermediate and slow acetylators, respectively, after tradeoff between efficacy and the occurrence of side effects.

14.
Chemosphere ; 246: 125842, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31927387

RESUMO

In nature, the calcium carbonate shows different interactions with different metal ions. Inspiration from this natural phenomenon, in this work, the selective recovery of heavy metals from wastewater by mechanically activated calcium carbonate was investigated. The changes in Ca2+ concentration, pH value and metals uptake ratio of solution showed that M2+ (M = Cu, Mn, Zn and Ni) were endowed with different migration rules, resulting in the various interaction with the calcium carbonate in metal-bearing solution. The combination of XRD, SEM, and stereomicroscope affirmed that the adsorbed M2+ rarely change the lattice structure of calcium carbonate, while the adsorbed Cu2+ and Zn2+ could convert the mineral phase from calcium carbonate to posnjakite and hydrozincite, respectively. As a result of phase transition, 15% Cu2+ and 6% Zn2+ were uptaken with initial concentration of 1 mM for 100 min, however, the unsatisfactory recovery prevented the efficient recycling of metal. The mechanically activated calcium carbonate had a superior solubility at the solid/liquid interface, promoting mineral phase transformation on the premise of weak displacement adsorption. Hence, the uptake ratio of Cu2+ and Zn2+ were significantly increased to 99% and 53% at the same condition. Finally, Cu2+ was recovered from polymetallic systems from complex environment with high precision. The concept of selective recycling in this research guides the development of innovative processes from natural information.

15.
Curr Microbiol ; 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31915985

RESUMO

Arctium lappa L. is widely used for medicinal purposes across China, and significant effort has been directed toward enhancing its quality. Association with microorganisms has been shown to influence both plant growth and metabolites, providing a possible avenue for its quality improvement. In this study, we investigated the microorganism compositions of the root, stem, leaf, fruit and rhizospheric soil of A. lappa through high-throughput Illumina sequencing of 16S rRNA genes and ITS regions. A total of 796,891 16S rRNA and 626,270 ITS reads were obtained from the samples. Analysis of the sequencing data revealed that bacterial and fungal communities were more diverse in the rhizospheric soil sample compared with other samples. Cyanobacteria, Actinobacteria, Proteobacteria, Firmicutes, and Bacteroidetes phyla were found in all samples. Cyanobacteria was particularly enriched in the root, stem, leaf and fruit at 88.59%, 86.15%, 98.31% and 93.57%, respectively; Actinobacteria was the highest in rhizospheric soil, at 37.53%. Ascomycota was the most dominant fungal phylum, representing 69.17%, 58.18%, 87.93%, 90.18% and 80.21% in the root, stem, leaf, fruit, and rhizospheric soil, respectively. Several novel unclassifiable bacterial and fungal species were also detected. In total, we detected about 922 bacterial and 334 fungal species, which include a number of unclassifiable species. Additionally, the root, stem, leaf, fruit and rhizospheric soil of A. lappa were sources for screening new bioactive metabolites.

16.
Environ Technol ; : 1-10, 2020 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-31990636

RESUMO

In a coagulation-flocculation process, optimal separation of the resultant aggregates plays a decisive role on coagulation performance and provides a lower burden for subsequent treatment units. This separation highly depends on the stability of the micro-flocs formed during the initial, rapid stage of coagulation. In this work, a two-stage addition of aluminium sulphate (alum, Al2(SO4)3) was employed by adding 0.04 and 0.08 mM Al2(SO4)3 at the beginning and the end of rapid mixing, respectively. The coagulation performance and floc characteristics were compared to conventional single addition with the same total coagulant dosage, and the effects of variable rapid-mixing speeds (160-850 rpm) and duration time (10-120 s) were investigated. The results showed that the residual turbidity of two-stage coagulant addition was 85.1% lower than single addition when applied at a mixing speed of 580 rpm and a duration time of 120 s. The underlying coagulation mechanism revealed that the two-step addition more effectively neutralized colloids and formed larger aggregates that settled better and could more easily be removed. Moreover, the aggregates were less firm, which was attributed to different interactions among the micro-flocs, the second addition of Al2(SO4)3 and destabilized colloids. The present work provides data to broaden the window of rapid-mixing environments for more effective coagulation.

17.
Trials ; 21(1): 102, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31959220

RESUMO

BACKGROUND: Hysterosalpingography (HSG) is an accepted radiologic diagnostic modality for initial infertility workup, and is generally considered uncomfortable and painful. However, the management of pain related to HSG remains inefficient. As an emerging nonpharmacologic and noninvasive pain control strategy, virtual reality (VR) distraction has been successfully used in areas such as burns, blunt force trauma, hospital-based needle procedures, dental/periodontal procedures, and urological endoscopy patients. This study aims to evaluate the analgesic effect of VR during HSG. METHODS/DESIGN: A single-center, parallel-group, randomized controlled trial will be carried out in the Radiology Department of Yinchuan Women and Children Healthcare Hospital, Yinchuan. A total of 200 participants who are scheduled for HSG will be enrolled in this study. The participants will be randomized (1:1) into two groups: a VR group and a blank control group. The VR group will receive routine care plus immersive VR intervention and the blank control group will receive routine care. Outcomes will be monitored at baseline, immediately after HSG and 15 min after HSG for each group. The primary outcome is the worst pain score during HSG by a visual analog scale (VAS). The secondary outcomes include: affective pain, cognitive pain, and anxiety during the HSG procedure; worst pain within 15 min after HSG; patient satisfaction and acceptance with pain management; physiological parameters; adverse effects; HSG results; and immersion perception score of the VR system (for the VR condition only). DISCUSSION: This study will focus on exploring a simply operated, noninvasive and low-cost analgesia during the HSG procedure. The results of this trial will provide data on the feasibility and safety of VR distraction therapy during HSG. TRIAL REGISTRATION: Chinese Clinical Trial Register, ChiCTR1900021342. Registered on 16 February 2019.

18.
Talanta ; 210: 120651, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31987189

RESUMO

MicroRNAs (miRNAs) are important biomarkers because their abnormal expressions or mutations can usually indicate the development of cancers or other diseases. However, their high family sequence homology and low abundance pose a major challenge for the analysis of miRNAs. In the present work, we developed a dual strand extension recycling signal amplification strategy for non-label detection of miRNA-155 with high sensitivity on the basis of DNA polymerase and the G-quadruplex/thioflavin T (ThT) complexes. The specific miRNA-155 target sequences could initiate two strand extension-based independent recycling cycles under the function of the DNA polymerase, resulting in the production of many active G-quadruplex segments. The dye of ThT further bound the G-quadruplexes to induce greatly enhanced fluorescence for ultra-sensitively detecting miRNA-155 sequences at the low concentration of 35 fM in the dynamic range from 0.1 pM to 100 pM. The proposed detection strategy used the completely unmodified and synthetic DNA as probes and could be performed in homogeneous solutions. This method with a high selectivity could also be used for diluted serum samples. The demonstration of our new method for miRNA detection thus offers it with high potentials for miRNA biomarker analyses with the purpose of clinical diagnostics and biomedical applications.

19.
J Mater Chem B ; 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31993604

RESUMO

The synthesis of superparamagnetic nanoclusters is critical for ultra-sensitive magnetic resonance imaging (MRI). Herein, we describe the synthesis of water-soluble, biocompatible and superparamagnetic gadolinium-doped iron oxide nanoclusters (GdIO NCs) via a one-pot reaction by thermal decomposition of ferric oleate and gadolinium oleate precursors with α,ω-dicarboxyl poly(ethylene glycol) as a surfactant. The resulting water-dispersible GdIO NCs possess good stability and monodispersity with narrow size distribution, and exhibit superparamagnetic behaviors. We also explored the effect of gadolinium doping amounts on the magnetic properties and longitudinal (r1) and transverse relaxivity (r2) of the nanoclusters. In addition, the GdIO NCs can be functionalized with fluorescein isothiocyanate (FITC) while maintaining their magnetic properties and biocompatibility. The GdIO NCs and FITC conjugated NCs were preliminarily evaluated as MRI and fluorescent probes. The results show that the GdIO NCs provide an important nano-platform for theranostics with non-invasive MRI and optical monitoring capabilities.

20.
Environ Technol ; : 1-44, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31996100

RESUMO

Biosorption is an emerging technology for the removal of heavy metals from industrial wastewater by natural or modified biomass. In this study, we proposed a novel protocol for making full use of seaweeds. Brown seaweed Sargassum carpophyllum residue (SCR) and green seaweed Caulerpa lentillifera residue (CLR) were obtained after extraction of the bioactive polysaccharides. The obtained residues were further chemical modified by butanedioic anhydride to obtained respective carboxylated product, named CSCR and CCLR. According to the titration results, CSCR and CCLR contained 2.77 mmol/g and 2.12 mmol/g of carboxyl group. After modification, the adsorption capacity for metal ions increased by 3-6 times. The adsorption capacity of CSCR for Cu2+, Pb2+, Cd2+ and Mn2+ was 52.37 mg/g, 107.11 mg/g, 85.62 mg/g, and 43.52 mg/g, and that of CCLR was 78.10 mg/g, 108.80 mg/g, 87.30 mg/g and 57.80 mg/g, respectively. The adsorption was well described by the pseudo-second order kinetic model and Langmuir adsorption isotherm equation.[Formula: see text].

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