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1.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(10): 1056-1059, 2019 Oct 06.
Artigo em Chinês | MEDLINE | ID: mdl-31607055

RESUMO

We analyzed the influenza surveillance data of Children's Hospital of Suzhou University from 2016 to 2018 and estimated the hospitalization burden of children under 5 years old due to influenza infection in Suzhou. The results showed that the influenza virus positive rate of 1 451 severe acute respiratory infection (SARI) cases in Children's Hospital of Suzhou University was 13.6% (95%CI: 11.8%-15.3%; 197 cases), among which the influenza pandemic intensity in 2017-2018 was relatively high, and A/H1N1 was the main pandemic virus. It was estimated that the hospitalization rate of influenza-related SARI in children under 5 years old in Suzhou was 6.9‰ (95%CI: 6.6‰-7.2‰), among which the hospitalization rate of children aged<6 months was higher, up to 11.4‰ (95%CI: 9.9‰-12.8‰).


Assuntos
Influenza Humana/epidemiologia , Infecções Respiratórias/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Hospitalização , Humanos , Lactente , Vírus da Influenza A Subtipo H1N1
2.
Eur Rev Med Pharmacol Sci ; 23(17): 7599-7604, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31539151

RESUMO

OBJECTIVE: The aim of the present work was to investigate the effects and mechanisms of long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) in cardiomyocytes injury and apoptosis induced by high glucose (HG) in vitro. MATERIALS AND METHODS: HG-induced rats' cardiomyocytes with si-MEG3 transfection were constructed. Cell viability and lactate dehydrogenase (LDH) level were examined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and LDH assay kits, respectively. Cardiomyocytes apoptosis was detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) staining. The expressions of Bcl-2, Bax, cleaved caspase-9 and cleaved caspase-3 proteins were determined by Western blot. The expression of lncRNA MEG3 was measured by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). RESULTS: Our results indicated that the expression of MEG3 was significantly upregulated in HG-treated cardiomyocytes. The downregulation of MEG3 could attenuate cardiomyocytes injury and apoptosis by decreasing the Bax/Bcl-2 ratio, cleaved caspase-9 and cleaved caspase-3 expression. CONCLUSIONS: The downregulation of MEG3 could attenuate cardiomyocytes injury and apoptosis induced by HG. The molecular mechanism was associated with the inhibition of the mitochondria-mediated apoptosis pathway.

4.
Phys Rev Lett ; 122(19): 192503, 2019 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-31144958

RESUMO

A new short-lived neutron-deficient isotope ^{220}Np was synthesized in the fusion-evaporation reaction ^{185}Re(^{40}Ar,5n)^{220}Np at the gas-filled recoil separator SHANS. Based on the measurement of the correlated α-decay chains, the decay properties of ^{220}Np with E_{α}=10040(18) keV and T_{1/2}=25_{-7}^{+14} µs were determined, which are in good agreement with theoretical predictions. From the new experimental results coupled with the recently reported α-decay data of ^{219,223}Np, the α-decay systematics for Np isotopes around N=126 was established, which allows us for the first time to test the robustness of the N=126 shell closure in Z=93 Np isotopes. The results also indicate that, in the region of nuclei with Z≥83, the proton drip line has been reached for all odd-Z isotopes up to Np.

5.
Zhonghua Gan Zang Bing Za Zhi ; 26(10): 792-796, 2018 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-30481891

RESUMO

Liver fibrosis is a common pathological response in chronic liver injury. In the pathological process of hepatic injury, signaling pathways associated with hepatic fibrosis, which mediates the repair, proliferation and fibrosis of the liver secrete different cytokines. In these pathways, transforming growth factor beta (TGFß) and signal transducer and activator of transcription 3 (STAT3) play key roles in the proliferation and activation of hepatic stellate cells (HSCs) and promote epithelial mesenchymal transition. In addition, it is also involved in the process of proliferation and transformation of collagen and extracellular matrix molecules into myofibroblasts. TGFß and STAT3 molecular-related signaling pathways mediate the loss of epithelial phenotype and gene expression in mature epithelial cells, transforming them into mesenchymal cells, and producing anti-apoptosis to hepatocytes and promoting the proliferation of HSCs. However, the mechanisms by which STAT3 and TGFß molecules are involved in the development and progression of liver fibrosis are not sound distinct. In this review, we attempt to know the mechanisms and interactions of TGFß and STAT3 molecules that mediate potential liver fibrosis, and promote their role in promoting HSCs production and epithelial mesenchymal transition.


Assuntos
Células Estreladas do Fígado , Cirrose Hepática/diagnóstico , Fator de Transcrição STAT3 , Fator de Crescimento Transformador beta , Humanos , Fígado , Fator de Crescimento Transformador beta1
6.
Zhonghua Yi Xue Za Zhi ; 98(44): 3607-3612, 2018 Nov 27.
Artigo em Chinês | MEDLINE | ID: mdl-30486579

RESUMO

Objective: To evaluate the performance of MALDI Biotyper system in identification of clinically isolated pathogens so as to provide a new rapid identification method. Methods: Total 21 270 pathogens strains, isolated from the First Affiliated Hospital of Fujian Medical Universityduring Nov. 2015 to Dec. 2016, were identified by VITEK-Ⅱ, API and MALDI Biotyper system, respectively.The isolated strains were confirmed by DNA sequencing. Results: The identification of common bacteria with MALDI Biotyper and phenotypic system is highly consistent (>95% and >90%). Among 43 strains of anaerobic bacteria, MALDI Biotyper could identify 90.7% bacteria to species level and 97.7% bacteria to genus level with the statistical significance(χ(2)=6.76, P<0.01), while phenotypic system only identified 65.1% bacteria to species and 69.8% bacteria to genus. Also, no statistical significance was shown for Trichosporon and Candida(P>0.05). MALDI Biotyper could identify 76% filamentous fungi and all of Actinomycetes, Nocardia, Mycobacterium and Legionella to genus level. Conclusions: MALDI Biotyper is an easy-performed, sensitive method for the identification of clinically isolated pathogens. Additionally, the pretreatment and reference database has the effect on identification.


Assuntos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Fungos , Legionella , Mycobacterium , Análise de Sequência de DNA
7.
Zhonghua Fu Chan Ke Za Zhi ; 53(6): 390-395, 2018 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-29961281

RESUMO

Objective: To explore the clinical significance of centralized surveillance of hydatidiform mole. Methods: From Feb. 2013 to Feb. 2017 all patients with hydatidiform mole, who underwent suction curettage and were confirmed by histopathology in Dalian Maternal and Child Health Care Hospital, were registered centrally for serum hCG monitoring and treatment if necessary. Prophylactic chemotherapy was not administered regardless of risk factors for malignant transformation of hydatidiform mole. The risk factors included age of over 40 years, excessive uterine enlargement for presumed gestational age, a serum hCG level greater than 5 00 000 U/L, large theca lutein ovarian cysts (>6 cm), and a history of previous hydatidiform mole. The centralized surveillance of hydatidiform mole was based on the central pathology review, team cooperation and service improvement. Their treatments and outcomes were analyzed retrospectively. Results: A total of 407 women of hydatidiform mole were registered with histopathology confirmation, including 70 high-risk hydatidiform moles. The follow-up rate was 97.5% (397/407) . The incidence of post-mole neoplasia was 8.1% (32/397) , which was diagnosed in 22.9% (16/70) of high-risk and in 4.9% (16/327) of low-risk hydatidiform moles, showed statistically significant difference between high-risk and low-risk groups (χ(2)=25.108, P<0.01) . Thirty-two patients with post-mole neoplasia were all at low risk of International Federation of Gynecology and Obstetrics (FIGO) score (range, 0-6) and received complete remission with chemotherapy alone in 31 of them except one treated by hysterectomy. The primary cure rate of single-agent chemotherapy was 60.0% (18/30) . Patients with low-risk or high-risk post-mole neoplasia were both 16. There were no significant differences between the two groups in interval that was end of antecedent pregnancy to start of treatment, the serum level of hCG before treatment, clinical stage or risk factor score (all P>0.05) . Conclusions: The risk of malignant transformation is increased in high-risk hydatidiform mole, however, the high risk factor itself does not affect the prognosis in patients with timely diagnosis and treatment of post-mole neoplasia. Therefore, prophylactic chemotherapy is not recommended to high-risk hydatidiform mole patients. Centralized surveillance of hydatidiform mole is practical in a local hospital of China and could greatly improve the prognosis of post-mole neoplasia.


Assuntos
Mola Hidatiforme/epidemiologia , Neoplasias Uterinas/epidemiologia , Adulto , China/epidemiologia , Feminino , Idade Gestacional , Humanos , Mola Hidatiforme/patologia , Mola Hidatiforme/terapia , Histerectomia , Incidência , Pessoa de Meia-Idade , Gravidez , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Neoplasias Uterinas/patologia , Neoplasias Uterinas/terapia
8.
Eur Rev Med Pharmacol Sci ; 22(6): 1798-1811, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29630129

RESUMO

OBJECTIVE: Non-cardiac surgery is associated with perioperative cerebral complications (delirium, postoperative cognition dysfunction, stroke). While rare, these complications can lead to disabilities and deaths. Information is ambiguous as to whether pharmacological preoperative treatment exerts neuroprotection. We wished to systematically assess potential modulation by statins, lidocaine, ketamine or magnesium sulfate of the relative risk of cerebral complications in noncardiac surgery. Selection of these pharmacological agents was based on their known neuroprotective abilities. PATIENTS AND METHODS: By searching Medline, EMBASE and Cochrane databases, we identified 4 suitable publications that collectively enrolled 1358 patients (intent-to-treat population), of which 679 patients were treated preoperatively with statins (404 patients on atorvastatin and 275 on rosuvastatin) and 679 patients with preoperative placebo. The reported cerebral outcome was stroke, assessed either within 30 days (4 publications) or 6 months (2 publications) after surgery. RESULTS: Episodes of stroke within 30 days and 6 months postoperatively were observed in several publications, enabling aggregate analyses. No modulation by statins of the relative risk of stroke at 30 days was observed (risk ratio 1.59, 95% confidence interval 0.08-30.97; p = 0.76). At 6 months, statins showed an insignificant trend toward neuroprotection (risk ratio 0.33, 95% confidence interval 0.05-2.10; p = 0.24). CONCLUSIONS: The available clinical data are still scarce. Our analyses indicate no protective effects by statins against perioperative stroke but some favorable trends toward delayed stroke. Further randomized trials are needed to unequivocally assess the neuroprotective potential of current pharmacological agents in non-cardiac surgery.


Assuntos
Inibidores de Hidroximetilglutaril-CoA Redutases/farmacologia , Ketamina/farmacologia , Lidocaína/farmacologia , Sulfato de Magnésio/farmacologia , Fármacos Neuroprotetores/farmacologia , Complicações Pós-Operatórias/prevenção & controle , Acidente Vascular Cerebral/prevenção & controle , Humanos , Período Perioperatório
9.
Zhonghua Wai Ke Za Zhi ; 56(4): 284-288, 2018 Apr 01.
Artigo em Chinês | MEDLINE | ID: mdl-29562414

RESUMO

Objective: To evaluate the incidence of postoperative venous thromboembolism (VTE) after thoracic surgery and its characteristic. Methods: This was a single-center, prospective cohort study. Patients undergoing major thoracic surgeries between July 2016 and March 2017 at Department of Thoracic Surgery, Beijing Chaoyang Hospital Affiliated to Capital Medical University were enrolled in this study. Besides the routine examination, all patients were screened for deep venous thrombosis (DVT) by using noninvasive duplex lower-extremity ultrasonography after surgery. CT pulmonary angiography (CTPA) was carried out if patients had one of the following conditions including typical symptoms of PE, high Caprini score (>9 points) or new diagnosed postoperative DVT. Caprini risk assessment model was used to detect high risk patients. No patients received any prophylaxis of VTE before surgery. Further data was analyzed for identifying the incidence of postoperative VTE. The t-test, χ2 test or Wilcoxon rank-sum test was used to analyze the quantitative data and classification data, respectively. Results: Totally 345 patients who undergoing major thoracic surgery were enrolled in this study including 145 benign diseases and 200 malignant diseases.There were 207 male and 138 female, aging from 15 to 85 years. Surgery procedures included 285 lung surgeries, 27 esophagectomies, 22 mediastinal surgeries and 11 other procedures. The overall incidence of VTE was 13.9% (48 of 345) after major thoracic surgery including 39 patients with newly diagnosed DVT (81.2%), 1 patient with PE (2.1%) and 8 patients with DVT+ PE (16.7%). The median time of VTE detected was 4.5 days postoperative. There were 89.6% (43/48) VTE cases diagnosed in 1 week. The incidence of VTE was 9.0% in patients with benign diseases, while 17.5% in malignant diseases (χ2=5.112, P<0.05). The incidence of VTE in patients with pulmonary diseases was 12.6%, among that, in patients with lung cancer and benign lung diseases was 16.4% and 7.5 % (χ2=4.946, P<0.05), respectively. Regarding to Caprini risk assessment model, the incidence of VTE in low risk patients, moderate risk patients (Caprini score 5 to 8 points)and high risk patients(≥9 points)were 0(0/77), 15.2%(33/217) and 29.4%(15/51), respectively(Z=-12.166, P<0.05). In patients with lung cancer, 98.2% of patients were moderate risk or high risk; only 3 cases scored low risk. The incidence of VTE in moderate risk and high risk patients was 13.4%(18/134) and 32.1%(9/28), respectively, while it was 0(0/3) in low risk patients. Conclusions: The overall incidence of VTE after major thoracic surgeries is 13.9%, and the incidence of VTE after lung cancer surgeries was 16.4%. Most of the VTE cases occurr within one week after the surgery. Caprini risk assessment model can identify high risk patients effectively.


Assuntos
Procedimentos Cirúrgicos Torácicos , Tromboembolia Venosa , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Embolia Pulmonar , Medição de Risco , Fatores de Risco , Procedimentos Cirúrgicos Torácicos/efeitos adversos , Tromboembolia Venosa/etiologia , Trombose Venosa , Adulto Jovem
10.
Insect Mol Biol ; 27(3): 365-372, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29484744

RESUMO

Identifying the Single Nucleotide Polymorphisms (SNPs) with functions in insect fecundity promises to provide novel insight into genetic mechanisms of adaptation and to aid in effective control of insect populations. We previously identified several SNPs within the vitellogenin (Vg) promoter region between a high-fecundity population (HFP) and a low-fecundity population (LFP) of the brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae). Here, we found that an A-to-T (HFP allele to LFP allele) transversion at nucleotide -953 upstream of Vg in a Nilaparvata lugens GATA-1 (NlGATA-1) binding motif is associated with the level of Vg transcription. We also characterized NlGATA-1, containing a double CX2 CX17 CX2 C zinc finger, which has been implicated in the activation of Vg gene expression. Knockdown of the NlGATA-1 gene results in a reduced basal level of expression of the Vg gene and fewer offspring of N. lugens in vivo, whereas overexpression of NlGATA-1 in cells increased Vg promoter activity. Moreover, upon cotransfection with NlGATA-1 expression vector, the luciferase activities of Vg reporter vectors with the A allele were significantly higher than those with the T allele. These findings support a mechanism in which a SNP within the promoter of Vg is associated with the level of Vg transcription by altering the binding activity of NlGATA-1 and subsequently affecting fecundity in N. lugens.


Assuntos
Fator de Transcrição GATA1/genética , Regulação da Expressão Gênica/genética , Hemípteros/fisiologia , Proteínas de Insetos/genética , Polimorfismo de Nucleotídeo Único , Vitelogeninas/genética , Animais , Sequência de Bases , Fertilidade/genética , Fator de Transcrição GATA1/metabolismo , Hemípteros/genética , Hemípteros/metabolismo , Proteínas de Insetos/metabolismo , Vitelogeninas/metabolismo
11.
Bull Entomol Res ; 108(5): 667-673, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29223179

RESUMO

Some fire ants of the genus Solenopsis have become invasive species in the southern United States displacing native species by competition. Although the displacement pattern seems clear, the mechanisms underlying competitive advantage remain unclear. The ability of ant workers to produce relatively larger amount of alarm pheromone may correspond to relative greater fitness among sympatric fire ant species. Here we report on quantitative intra-specific (i.e. inter-caste) and inter-specific differences of alarm pheromone component, 2-ethyl-3,6-dimethylpyrazine (2E36DMP), for several fire ant species. The alarm pheromone component was extracted by soaking ants in hexane for 48 h and subsequently quantified by gas chromatography-mass spectrometry at single ion monitoring mode. Solenopsis invicta workers had more 2E36DMP than male or female alates by relative weight; individual workers, however, contained significantly less pyrazine. We thus believe that alarm pheromones may serve additional roles in alates. Workers of Solenopsis richteri, S. invicta, and hybrid (S. richteri × S. invicta) had significantly more 2E36DMP than a native fire ant species, Solenopsis geminata. The hybrid fire ant had significantly less 2E36DMP than the two parent species, S. richteri and S. invicta. It seems likely that higher alarm pheromone content may have favored invasion success of exotic fire ants over native species. We discuss the potential role of inter-specific variation in pyrazine content for the relationship between the observed shifts in the spatial distributions of the three exotic fire ant species in southern United States and the displacement of native fire ant species.


Assuntos
Formigas/química , Feromônios/química , Animais , Formigas/metabolismo , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Espécies Introduzidas , Masculino , Feromônios/metabolismo , Especificidade da Espécie
12.
Sci Rep ; 7(1): 16243, 2017 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-29176552

RESUMO

The response of the open two-band system to external fields would in general be different from that of a strictly isolated one. In this paper, we systematically study the Hall conductance of a two-band model under the influence of its environment by treating the system and its environment on equal footing. In order to clarify some well-established conclusions about the Hall conductance, we do not use the rotating wave approximation (RWA) in obtaining an effective Hamiltonian. Specifically, we first derive the ground state of the whole system (the system plus the environment) beyond the RWA, then calculate an analytical expression for Hall conductance of this open system in the ground state. We apply the expression to two examples, including a magnetic semiconductor with Rashba-type spin-orbit coupling and an electron gas on a square two-dimensional lattice. The calculations show that the transition points of topological phase are robust against the environment. Our results suggest a way to the controlling of the whole system response, which has potential applications for condensed matter physics and quantum statistical mechanics.

13.
Eur Rev Med Pharmacol Sci ; 21(4): 848-854, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28272696

RESUMO

OBJECTIVE: To investigate the relationship between the expression levels of microRNA-208b and early diagnosis of the acute myocardial infarction. MATERIALS AND METHODS: Literature search were performed within PubMed, Medline, Embase, and CNKI databases, according to inclusion and exclusion criteria, and other relevant literatures about included studies were searched to get more information about this study. Two staff members extracted data from the included studies for meta-analysis. RESULTS: This study included 6 documents, involving a total of 826 patients with acute myocardial infarction and 429 controls. Meta-analysis results: sensitivity 82%, specificity 83%, positive likelihood ratio of 5.0, negative likelihood ratio 0.21, diagnostic odds ratio area was 23, area under operating characteristic curve was 0.88 (95% CI: 0.85-0.91). CONCLUSIONS: microRNA-208b expression was increased in the blood of patients with acute myocardial infarction, and may be used for the early diagnosis of acute myocardial infarction molecular biomarkers.


Assuntos
MicroRNAs/sangue , Infarto do Miocárdio/sangue , Biomarcadores/sangue , Humanos , Infarto do Miocárdio/diagnóstico , Razão de Chances , Sensibilidade e Especificidade
14.
Genet Mol Res ; 15(1): 15018232, 2016 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-27051040

RESUMO

We performed an exploratory study by analyzing the correlation of 46, XY disorders of sex development (46, XY DSD) with androgen receptor (AR) and steroid 5α-reductase-2 (SRD5A2) gene mutations and a safety analysis of dihydrotestosterone (DHT) gel treatment for pediatric micropenis. We collected samples from 76 pediatric patients with 46, XY DSD and 50 healthy adult men with normal fertility as the control group. The pediatric patients were treated with DHT gel (0.1-0.3 mg/kg/day) for three to six months. The extended penis length, testicular volume, and multiple blood parameters were collected before treatment and one, three, and six months after treatment. Of the 76 cases with 46, XY DSD, 31.58% had hypospadias with micropenis and 6.58% had male pseudohermaphroditism. Through AR gene screening, it was found that 14 patients had AR point mutations and 22 patients had SRD5A2 mutations. After treatment with DHT, the penis length of the patients significantly improved after one, three, and six months of treatment, with longer treatment times resulting in greater improvement. Before treatment with DHT, the average serum DHT value of patients with 46, XY DSD was 24.29 pg/mL. After one, three, and six months of treatment, this value increased to 430.71, 328.9, and 323.6 pg/mL, respectively. We conclude that for pediatric patients who have male hermaphroditism or hypospadias with micropenis, AR and SRD5A2 gene mutation detection should be performed. Local application of DHT gel can promote penis growth effectively without systemic adverse reactions.


Assuntos
3-Oxo-5-alfa-Esteroide 4-Desidrogenase/genética , Transtorno 46,XY do Desenvolvimento Sexual/metabolismo , Hipospadia/metabolismo , Proteínas de Membrana/genética , Mutação , Receptores Androgênicos/genética , Adulto , Criança , China , Di-Hidrotestosterona/sangue , Di-Hidrotestosterona/uso terapêutico , Transtorno 46,XY do Desenvolvimento Sexual/complicações , Transtorno 46,XY do Desenvolvimento Sexual/genética , Testes Genéticos , Doenças dos Genitais Masculinos/sangue , Doenças dos Genitais Masculinos/tratamento farmacológico , Doenças dos Genitais Masculinos/etiologia , Humanos , Hipospadia/etiologia , Hipospadia/genética , Masculino , Pênis/anormalidades
15.
Genet Mol Res ; 14(2): 3474-80, 2015 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-25966114

RESUMO

We investigated the association between rs751141 polymorphisms in the EPHX2 gene and essential hypertension in Uygur, Kazakh, and Han subjects in Xinjiang, China. A total of 302 essential hypertensive patients in Uygur, 267 in Kazakh, and 368 in Han, as well as 323 normotensive controls in Uygur, 284 in Kazakh, and 348 in Han were enrolled in this study. The TaqMan assay was used to detect the rs751141 G/A gene polymorphism in EPHX2. The rs751141 G/A genotype frequencies for the GA+AA genotypes were 40.2% in essential hypertensive subjects and 52.0% in control subjects in the Han population. The frequencies were significantly different between the 2 Han groups (P < 0.01). The rs751141G/A gene polymorphism showed no significant difference between essential hypertensive patients and normotensive controls in Kazakh and Uygur (all P > 0.05). Essential hypertension in Xinjiang was associated with the rs751141 G/A allele gene polymorphism in EPHX2 in Han subjects but not in Kazakh and Uygur subjects. The rs751141 allele gene polymorphism may be an independent protective factor against essential hypertension in the Han population.


Assuntos
Epóxido Hidrolases/genética , Hipertensão/genética , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China , Hipertensão Essencial , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Hipertensão/etnologia , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único
16.
Lett Appl Microbiol ; 59(6): 580-6, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25163827

RESUMO

UNLABELLED: Clostridium sporogenes ATCC 3584 is an obligate anaerobe that has been reported to possess excellent tumour-targeting capacity. Here, we use Cl. sporogenes as a vector to deliver IL-12, a potent antitumour cytokine that bears numerous antitumour properties but that has limited clinical applications due to its strong toxicity when delivered systemically. In this study, Cl. sporogenes was genetically engineered to secrete murine IL-12, and its antitumour efficacy and toxicity were investigated in a murine EMT6 mammary carcinoma model. After intravenous injection, Cl. sporogenes was able to selectively settle and reproduce in the tumours without encroaching on normal tissues, resulting in a clear delay of tumour growth and a 14·3% cure rate. Importantly, the mice showed no obvious toxicity-associated side effects, such as diarrhoea and weight loss, during the treatment process. The significant antitumour efficacy and low toxicity of this treatment may be explained by the selective tumour-targeting properties of Cl. sporogenes and by the sustained release of IL-12 accompanying bacterial proliferation. This moderate local IL-12 concentration would not induce the severe response in the entire body, that is inevitable when IL-12 is administered directly. SIGNIFICANCE AND IMPACT OF THE STUDY: Interleukin-12 (IL-12) is a potent antitumour cytokine, but it is toxic when administrated systemically. This study demonstrates that murine IL-12 can be systemically delivered to hypoxic sites in solid tumours by Clostridium sporogenes, producing a clear delay in tumour growth and a 14·3% cure rate in a mouse tumour model. Importantly, there is no obvious toxicity associated with IL-12 during the treatment process. This result may be accounted for by the excellent tumour-targeting capacity of Cl. sporogenes, targeting IL-12 directly to the tumour site instead of to the entire body.


Assuntos
Clostridium/genética , Interleucina-12/metabolismo , Neoplasias Mamárias Experimentais/microbiologia , Neoplasias Mamárias Experimentais/terapia , Animais , Hipóxia Celular , Clostridium/crescimento & desenvolvimento , Clostridium/metabolismo , Feminino , Terapia Genética , Vetores Genéticos , Interferon gama/biossíntese , Interferon gama/sangue , Interleucina-12/genética , Neoplasias Mamárias Experimentais/imunologia , Camundongos Endogâmicos BALB C , Proteínas Recombinantes/metabolismo
17.
Genet Mol Res ; 12(3): 3256-62, 2013 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-24065666

RESUMO

Many mitochondrial DNA sequences are used to estimate phylogenetic relationships among animal taxa and perform molecular phylogenetic evolution analysis. With the continuous development of sequencing technology, numerous mitochondrial sequences have been released in public databases, especially complete mitochondrial DNA sequences. Using multiple sequences is better than using single sequences for phylogenetic analysis of animals because multiple sequences have sufficient information for evolutionary process reconstruction. Therefore, we performed phylogenetic analyses of 14 species of Felidae based on complete mitochondrial genome sequences, with Canis familiaris as an outgroup, using neighbor joining, maximum likelihood, maximum parsimony, and Bayesian inference methods. The consensus phylogenetic trees supported the monophyly of Felidae, and the family could be divided into 2 subfamilies, Felinae and Pantherinae. The genus Panthera and species tigris were also studied in detail. Meanwhile, the divergence of this family was estimated by phylogenetic analysis using the Bayesian method with a relaxed molecular clock, and the results shown were consistent with previous studies. In summary, the evolution of Felidae was reconstructed by phylogenetic analysis based on mitochondrial genome sequences. The described method may be broadly applicable for phylogenetic analyses of anima taxa.


Assuntos
Felidae/genética , Genoma Mitocondrial , Filogenia , Animais , DNA Mitocondrial/genética , Cães , Evolução Molecular , Mitocôndrias/genética , Análise de Sequência de DNA
18.
Genet Mol Res ; 11(1): 628-35, 2012 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-22535398

RESUMO

Mitochondrial DNA sequences can be used to estimate phylogenetic relationships among animal taxa and for molecular phylogenetic evolution analysis. With the development of sequencing technology, more and more mitochondrial sequences have been made available in public databases, including whole mitochondrial DNA sequences. These data have been used for phylogenetic analysis of animal species, and for studies of evolutionary processes. We made phylogenetic analyses of 19 species of Cervidae, with Bos taurus as the outgroup. We used neighbor joining, maximum likelihood, maximum parsimony, and Bayesian inference methods on whole mitochondrial genome sequences. The consensus phylogenetic trees supported monophyly of the family Cervidae; it was divided into two subfamilies, Plesiometacarpalia and Telemetacarpalia, and four tribes, Cervinae, Muntiacinae, Hydropotinae, and Odocoileinae. The divergence times in these families were estimated by phylogenetic analysis using the Bayesian method with a relaxed molecular clock method; the results were consistent with those of previous studies. We concluded that the evolutionary structure of the family Cervidae can be reconstructed by phylogenetic analysis based on whole mitochondrial genomes; this method could be used broadly in phylogenetic evolutionary analysis of animal taxa.


Assuntos
Cervos/classificação , Cervos/genética , Evolução Molecular , Genoma Mitocondrial , Filogenia , Animais
19.
J Med Entomol ; 47(6): 1141-5, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21175065

RESUMO

The house fly, Musca domestica L. (Diptera: Muscidae), is involved in phoretic movement of pathogenic agents, but it has a very efficient defense mechanism against infection. It is believed that antimicrobial peptides play a significant role in the defense system of the house fly. Here, we isolated a peptide from the immunized house fly pupae, measured its molecular mass (3987.6 Da) by matrix-assisted laser desorption ionization/time of flight-mass spectrometry, and determined its amino acid sequence by using the Procise Protein Sequencing System (Applied Biosystems, Foster City, CA). The peptide was confirmed as a member of the insect defensin family. It displayed high activity against gram-positive bacteria but lower activity against gram-negative bacteria and fungi. Reverse transcription-polymerase chain reaction showed that the house fly defensin gene was constitutively expressed in naive pupae and strongly up-regulated after immunization. House fly defensin is an amphiphilic peptide with a structure similar to that of the CSalphabeta scaffold of insect defensin A from the flesh fly, Phormia terraenovae Robineau-Desvoidy. To our knowledge, this is the first isolated and characterized house fly antimicrobial peptide, and our work may provide useful information for developing pharmacologically active antimicrobial agents.


Assuntos
Peptídeos Catiônicos Antimicrobianos/metabolismo , Peptídeos Catiônicos Antimicrobianos/farmacologia , Dípteros/imunologia , Dípteros/metabolismo , Animais , Peptídeos Catiônicos Antimicrobianos/isolamento & purificação , Bactérias/efeitos dos fármacos , Fungos/efeitos dos fármacos
20.
Theor Appl Genet ; 121(5): 951-9, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20526576

RESUMO

A major limiting factor for high productivity of maize (Zea mays L.) in dense planting is light penetration through the canopy. Plant architecture with a narrower leaf angle (LA) and an optimum leaf orientation value (LOV) is desirable to increase light capture for photosynthesis and production per unit area. However, the genetic control of the plant architecture traits remains poorly understood in maize. In this study, QTL for LA, LOV, and related traits were mapped using a set of 229 F(2:3) families derived from the cross between compact and expanded inbred lines, evaluated in three environments. Twenty-five QTL were detected in total. Three of the QTL explained 37.4% and five of the QTL explained 53.9% of the phenotypic variance for LA and LOV, respectively. Two key genome regions controlling leaf angle and leaf orientation were identified. qLA1 and qLOV1 at nearest marker umc2226 on chromosome 1.02 accounted for 20.4 and 23.2% of the phenotypic variance, respectively; qLA5 and qLOV5 at nearest bnlg1287 on chromosome 5 accounted for 9.7 and 9.8% of the phenotypic variance, respectively. These QTL could provide useful information for marker-assisted selection in improving performance of plant architecture with regard to leaf angle and orientation.


Assuntos
Mapeamento Cromossômico , Folhas de Planta/anatomia & histologia , Folhas de Planta/genética , Locos de Características Quantitativas/genética , Zea mays/anatomia & histologia , Zea mays/genética , Agricultura , Cruzamentos Genéticos , Ligação Genética , Repetições de Microssatélites/genética , Característica Quantitativa Herdável
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