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1.
New Phytol ; 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35538666

RESUMO

Plant architecture is a key determinant of crop productivity and adaptation. The highly conserved microRNA319 (miR319) family functions in various biological processes, but little is known about how miR319 regulates plant architecture in wheat (Triticum aestivum). Here, we determined that the miR319/TaGAMYB3 module controls plant architecture and grain yield in common wheat. Repressing tae-miR319 using short tandem target mimics resulted in favorable plant architecture traits, including increased plant height, reduced tiller number, enlarged spikes and flag leaves, and thicker culms, as well as enhanced grain yield in field plot tests. Overexpressing tae-miR319 had the opposite effects on plant architecture and grain yield. Although both TaPCF8 and TaGAMYB3 were identified as miR319 target genes, genetic complementation assays demonstrated that only miR319-resistant TaGAMYB3 (rTaGAMYB3) abolished tae-miR319-mediated growth inhibition of flag leaves and spikes. TaGAMYB3 functions as a transcriptional activator of downstream genes including TaPSKR1, TaXTH23, TaMADS5, and TaMADS51 by binding to their promoters. Furthermore, TaGAMYB3 physically interacts with TaBA1, an important regulator of spike development, to additively activate the transcription of downstream genes such as TaMADS5. Our findings provide insight into how the miR319/TaGAMYB3 module regulates plant architecture and improves grain yield in common wheat.

2.
Data Brief ; 42: 108152, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35496493

RESUMO

This paper presents the data of chemical composition of the particles from OH oxidation reaction of 1,3,5-trimethylbenzene (1,3,5-TMB). The particle-phase compositions are measured on-line by using a vacuum ultraviolet (VUV) photoionization aerosol mass spectrometer. The assignments of the major peaks of photoionization mass spectrum, as well as their molecular structures, are presented. The optimized structures of the reactants, intermediates and transition states involved in the reaction of the bicyclic peroxy radical with HO2 are shown. The reaction routes of the OH-initiated oxidation of the deuterated 1,3,5-TMB sample are also calculated and displayed for comparison. The data presented here is related to the paper "Direct observation of the particle-phase bicyclic products from OH-initiated oxidation of 1,3,5-trimethylbenzene under NOx-free conditions" by Lin et al. (2022).

3.
Front Oncol ; 12: 857714, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35433464

RESUMO

Located on chromosome 11q13.4, miR-139-5p has been confirmed by several studies as a possible attractive biomarker for cancer, including breast cancer, but its mechanism of correlation in different molecular subtypes of breast cancer has not been reported. In this study, comprehensive bioinformatics analysis was used to evaluate the expression of miR-139-5p in different molecular subtypes of breast cancer (luminal A, luminal B, HER2-enriched, and basal-like). The target genes of miR-139-5p were predicted by using an online database TargetScan and miRDB, and three key genes, FBN2, MEX3A, and TPD52, were screened in combination with differentially expressed genes in different molecular subtypes of breast cancer. The expression of the three genes was verified separately, and the genes were analyzed for pathway and functional enrichment. Bone marrow mesenchymal stem cells (BMSC) are another kind of highly plastic cell population existing in bone marrow besides hematopoietic stem cells. BMSC can affect the proliferation and migration of cancer cells, promote the metastasis and development of cancer, and regulate the tumor microenvironment by secreting exosome mirnas, thus affecting the malignant biological behavior of tumor cells. Finally, human bone marrow mesenchymal stem cells exosomes were obtained by ultracentrifugation, and the morphology of exosomes was observed by transmission electron microscopy. The expression of miR-139-5p in normal breast cells MCF-10A, human breast cancer cell line MDA-MB-231 cells, and BMSCs-derived exosomes were compared; the exosomes and MDA-MB-231 cells were co-cultured to observe their effects on the proliferation of the MDA-MB-231 cells. Human bone marrow mesenchymal stem cell-derived exosomes inhibited the growth of breast cancer cells and promoted the expression of FBN2, MEX3A, and TPD52 by transporting miR-139-5p.

4.
Sci Total Environ ; 835: 155443, 2022 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-35469866

RESUMO

The Mollisol region of Northeast China has a large soil organic carbon (SOC) storage which is important for maintaining soil fertility. SOC is susceptible to various environmental factors; however, the responses of SOC content to environmental factors in different soil layers of cropland remain unclear, particularly in deep soil layers. In this study, we collected 138 soil samples from the surface, subsurface, and subsoil layers among 46 sample sites with monocropping maize and intensive conventional tillage in this region. We assessed the relative importance and effect paths of 12 environmental factors (including geography, climate, and soil properties) on SOC content in different layers using redundancy analysis (RDA), structural equation model (SEM), and variation partitioning analysis (VPA). The VPA results showed that SOC content was mainly affected by climatic factors that explained 68% and 57% for the surface and subsurface layers, respectively. However, SOC content in the subsoil layer was greatly affected by soil properties that explained 27%. Furthermore, the SEMs results suggested that geographical factors indirectly affected SOC content by influencing the climatic factors. Mean annual temperature was the most important factor affecting SOC content directly or indirectly, and its negative effects significantly diminished with soil depth, as it explained 63%, 52%, and 17% of the variation in SOC content for the surface, subsurface and subsoil layers, respectively. In addition, the effects of soil water-holding capacity on SOC content also decreased with soil depth, whereas pH and clay content showed a contrasting pattern. This implies that pH and clay content play important roles in the sequestration of SOC in deep soil layers. Moreover, the organic C content within >53 µm aggregates was more sensitive to environmental factors. This study can be useful for forecasting SOC dynamics and establishing reasonable C management strategies under climate change conditions.

5.
Opt Express ; 30(9): 15238-15249, 2022 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-35473250

RESUMO

We report the development of an optical-feedback cavity-enhanced absorption spectroscopy (OF-CEAS) instrument for OH detection at 2.8 µm using a DFB diode laser. Two different approaches, symmetry analysis and wavelength modulation, were performed to achieve laser frequency locking to the cavity mode. Compared with the symmetry analysis method, the wavelength modulation method continuously locked the laser frequency to the cavity mode and eliminated decoupling the laser from the cavity mode. A detection sensitivity of 1.7×10-9 cm-1 was achieved in a 25 s sampling time and was about 3 times better than that of the symmetry analysis method. The corresponding OH detection limit was ∼ 2×108 molecule/cm3. Further improvement can be achieved by using higher reflectivity mirrors and other high-sensitivity approaches, such as frequency modulation spectroscopy and Faraday rotation spectroscopy.

6.
Mol Med Rep ; 25(5)2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35302170

RESUMO

Circular RNA (circRNA) molecules are noncoding RNAs with unique circular covalently closed structures that contribute to gene expression regulation, protein translation and act as microRNA sponges. circRNAs also have important roles in human disease, particularly tumorigenesis and antitumor processes. Methylation is an epigenetic modification that regulates the expression and roles of DNA and coding RNA and their interactions, as well as of noncoding RNA molecules. Previous studies have focused on the effects of methylation modification on circRNA expression, transport, stability, translation and degradation of circRNAs, as well as how circRNA methylation occurs and the influence of circRNAs on methylation modification processes. circRNA and methylation can also regulate disease pathogenesis via these interactions. In the present study, we define the relationship between circRNAs and methylation, as well as the functions and mechanisms of their interactions during disease progression.


Assuntos
MicroRNAs , RNA Circular , Carcinogênese , Regulação da Expressão Gênica , Humanos , Metilação , MicroRNAs/genética , MicroRNAs/metabolismo , RNA Circular/genética
7.
Anal Chem ; 94(7): 3368-3375, 2022 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-35143171

RESUMO

Accurate and sensitive measurements of NO2 play an extremely important role in atmospheric studies. Increasingly, studies require NO2 measurements with parts per trillion by volume (pptv-level) detection limits. Other desirable instrument attributes include ease of use, long-term stability, and low maintenance. In this work, we report the development of an amplitude-modulated multimode-diode-laser-based cavity-enhanced absorption spectroscopy (AM-CEAS) system operating at 406 nm that uses phase-sensitive detection for extremely sensitive NO2 detection. The laser was TTL-modulated at 35 kHz. The mirror reflectivity was determined to be 99.985% based on the ring-down time measurement. The cavity base length was 47.5 cm, giving an effective absorption pathlength of ∼3.26 km. AM-CEAS achieved a 1σ detection precision of 35 pptv in a 1 s data acquisition time (4.98 × 10-10 cm-1), over 4 times lower than that attained using a ring-down approach and the same optical system. The AM-CEAS precision improved to 8 pptv over a data acquisition time of 30 s (1.14 × 10-10 cm-1). The AM-CEAS method with the multimode diode laser integrates the advantages of high light injection efficiency like on-axis alignment cavity ring-down spectroscopy, low cavity-mode noise like off-axis alignment CEAS, and narrow-bandwidth high-sensitivity weak signal detection of modulation spectroscopy, providing a powerful, straightforward, and general method for ultrasensitive absorption and extinction measurements.


Assuntos
Lasers , Dióxido de Nitrogênio , Luz , Análise Espectral/métodos
8.
Front Cell Dev Biol ; 10: 817877, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35198560

RESUMO

Mesenchymal stem cell (MSC) transplantation has been widely used as a potential treatment for a variety of diseases. However, the contradiction between the low survival rate of transplanted cells and the beneficial therapeutic effects has affected its clinical use. Lysosomes as organelles at the center of cellular recycling and metabolic signaling, play essential roles in MSC homeostasis. In the first part of this review, we summarize the role of lysosomal acidification dysfunction in MSC senescence. In the second part, we summarize some of the potential strategies targeting lysosomal proteins to enhance the therapeutic effect of MSCs.

9.
Phys Chem Chem Phys ; 24(4): 2015-2021, 2022 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-35018921

RESUMO

We present a vacuum ultraviolet (VUV) photoionization study of the gas-phase sulfuric acid (H2SO4) molecule in the 11-14 eV energy range by using the method of synchrotron radiation-based double imaging photoelectron photoion coincidence (i2PEPICO) spectroscopy complemented with accurate theoretical calculations. The slow photoelectron spectrum (SPES) of H2SO4 has been acquired and the three electronic states of H2SO4+, X2A, A2A and B2A have been populated and assigned. The adiabatic ionization energy of the H2SO4 molecule towards the X2A cationic ground state is measured at 11.684 ± 0.006 eV, in accordance with high-level calculated findings. With increasing photon energies, the H2SO4+ cation dissociates into HSO3+ and OH fragments and their adiabatic appearance energy is measured at 13.498 ± 0.007 eV. Then, the enthalpies of formation for the species involved in the photoionization and dissociative photoionization have been determined through a thermochemical cycle.

10.
Huan Jing Ke Xue ; 43(1): 230-238, 2022 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-34989507

RESUMO

Based on the flow direction of the Fuhe River into Baiyangdian Lake, the impacted area of the Fuhe River was divided into 6 subareas, and sediments from 48 sites were collected in November 2020. The characteristics and risks of sediment nutrients and heavy metal pollution in these six subareas were investigated. The results showed that the average ω(TN), ω(TP), and ω(TOC) were 1841 mg·kg-1, 769 mg·kg-1, and 1.77%, respectively. The major heavy metals were Cd, Cu, Zn, Hg, and Pb, which were 3.73, 1.50, 1.42, 1.31, and 1.31 times the soil background values for Hebei Province, respectively. The TP and heavy metal (Cd, Cu, Zn, Hg, and Pb) content showed a decreasing trend from the Fuhe River estuary to the downstream Zaolinzhuang, whereas the TN and TOC content showed no marked trends. TN, TP, TOC, and heavy metals (Cd, Cu, Zn, Hg, and Pb) were enriched in surface sediments (0-10 cm). The TP content in the surface sediments (0-10 cm) of the Fuhe River estuary, Fuhe River estuary-Nanliuzhuang, and Nanliuzhuang subareas were heavily polluted; the Wangjiazhai and Guangdian subareas were moderately polluted; and the Zaolinzhuang subarea was slightly polluted. Cd and Hg were the major contributors to heavy metal pollution, which were at considerable risk and moderate risk levels, respectively. The heavy metals in surface sediments (0-10 cm) of the Fuhe River estuary, Fuhe River estuary-Nanliuzhuang, and Nanliuzhuang subareas were at a considerable risk level, and the sediments below 30 cm presented a low risk level. The leaching concentrations of heavy metals in sediments from the subarea of severe ecological risk level were far less than the identification standard values of leaching toxicity, suggesting that the sediments can be treated as general waste after dredging.


Assuntos
Metais Pesados , Poluentes Químicos da Água , China , Monitoramento Ambiental , Sedimentos Geológicos , Lagos , Metais Pesados/análise , Nutrientes , Medição de Risco , Poluentes Químicos da Água/análise
11.
Int J Gen Med ; 15: 645-660, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35082515

RESUMO

BACKGROUND: Testicular cancer severely affects male health, so finding effective diagnosis and prognostic indicators and exploring its pathogenesis are very important. PURPOSE: This study aims to explore the hub genes that play important roles in the occurrence and development of testicular germ cell tumor (TGCT). METHODS: Data were obtained from Gene Expression Omnibus datasets (GSE3218 and GSE1818) and verified in The Cancer Genome Atlas database and the Genotype-Tissue Expression database and the Human Protein Atlas database. A protein-protein interaction network was constructed to obtain hub genes. GEO2R, R software and packages were used to analyze differentially expressed genes (DEGs), receiver operating characteristic curve assessment, Cox regression analysis, Kaplan-Meier survival curve assessment, Gene Ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis, the relationship with clinicopathological information, gene set enrichment analysis, the correlation with immune cells' infiltration, and the expression in pan-cancers of the hub genes. RESULTS: PLK4, TRIP13, TPR, KIF18A, CDKN3, HMMR, PBK, PTTG1, CKS2, SYCP1, HSPA2, and MKI67 were selected as the hub genes. mRNA of PLK4, TRIP13, CDKN3, SYCP1, HSPA2, and MKI67 had high diagnostic values, and higher expression of CDKN3 and HSPA2 mRNA were poor prognostic factors for progression-free interval of TGCT. The hub genes involved organelle division and cell cycle, chromosome and centromeric region, heat shock protein binding, and more. Downregulated TPR and PLK4 were selected as research targets for continued study, and they may participate in multiple signaling pathways. The expression of TPR and PLK4 correlated with the infiltration of a variety of immune cells and differed in pan-cancers. CONCLUSION: The mRNA levels of multiple hub genes have high diagnostic and prognostic values for TGCT. TPR and PLK4 may play a role in the occurrence and development of TGCT through cancer-related signaling pathways.

12.
Sci Total Environ ; 809: 151136, 2022 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-34695472

RESUMO

Organic carbon (OC) emitted from biomass burning (BB) plays an important role in the global radiation budget. In this work, primary OC emitted from wood pyrolysis and combustion under nitrogen (N2) and air conditions in a tube furnace was investigated. The absorption spectra, chemical functional groups, and molecular compositions of OC were analyzed using UV-Vis-NIR spectrophotometer, Fourier transform infrared spectroscopy (FTIR), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), respectively. The light absorption properties showed that the mass absorption efficiency at 365 nm (MAE365) of methanol-soluble OC (MSOC) is 3.1-3.8 times higher than that of water-soluble OC (WSOC). Moreover, the MAE365 values derived from the N2 pyrolysis atmosphere are higher than that from the air atmosphere for both MSOC and WSOC. These results indicated that OC extracted by methanol has higher light absorption, especially for the OC emitted from the N2 pyrolysis atmosphere. Although the FTIR spectra showed identical functional groups for the OC from the air and N2 conditions, molecular compositions from the FT-ICR MS analysis presented significant differences. The molecular weight (MW), double bonds equivalent (DBE), DBE/C, and modified aromaticity index (AImod) of extracted OC showed higher values in MSOC than those in WSOC, and higher values under the N2 atmosphere than those under the air atmosphere. In addition, MAE365 showed positive correlations with MW (r = 0.94), DBE (r = 0.88), DBE/C (r = 0.96), and AImod (r = 0.97), whereas negative correlations with H/C (r = -0.97), O/C (r = -0.90), N/C (r = -0.88), and S/C (r = -0.93). These results indicated that molecules with larger MW and a high level of unsaturation and aromaticity present higher light absorption, while molecules with high elemental ratios of H/C, O/C, N/C, and S/C are adverse to light absorption.


Assuntos
Poluentes Atmosféricos , Carbono , Aerossóis/análise , Poluentes Atmosféricos/análise , Carbono/análise , Monitoramento Ambiental , Metanol , Material Particulado/análise , Pirólise , Água , Madeira/química
13.
Environ Sci Pollut Res Int ; 29(11): 16716-16726, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34655384

RESUMO

Baiyangdian Lake (BYD), a large shallow lake in North China, has complex water landscape patterns that are underlies spatial variations in water quality. In this study, we collected 61 water samples from three water landscapes (reed littoral zones, fish ponds, and open water) and analyzed them for water quality parameters, such as dissolved organic carbon (DOC), total nitrogen (TN), and total phosphorus (TP). Water landscape distribution (determined using remote sensing imagery) was then used to assess correlations between water quality parameters and water landscape proportion in differently scaled buffer zones. There was substantial variation across all subareas, with TN and TP concentrations ranging from 0.90 to 4.10 mg/L and 0.06 to 0.18 mg/L, respectively, in class IV of water quality as a whole. Spatial variations in water quality were mainly caused by water landscape distribution and external nutrient inputs. There were negative correlations between DOC, TN, and TP concentrations and the area proportion of reed littoral zones in the 300 and 500 m buffers. In contrast, DOC, TN, and TP concentrations were significantly positively correlated with the area proportion of fish ponds in the 100 m buffer. Furthermore, compared with reed littoral zones, a lower ratio of nitrogen to phosphorus and a higher proportion of dissolved organic nitrogen and tyrosine-like proteins were found in fish ponds. These effects were mainly attributed to the development of internal sediment loadings due to nutrient exchange across the sediment-water interface. Therefore, dredging-based sediment removal from fish ponds should be considered to suppress internal phosphorus loading and accelerate recovery of the BYD ecosystem.


Assuntos
Lagos , Poluentes Químicos da Água , China , Ecossistema , Monitoramento Ambiental , Nitrogênio/análise , Fósforo/análise , Poluentes Químicos da Água/análise , Qualidade da Água
14.
Neurocrit Care ; 36(1): 21-29, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34403122

RESUMO

BACKGROUND: Cerebral autoregulation plays an important role in safeguarding adequate cerebral perfusion and reducing the risk of secondary brain injury, which is highly important for patients in the neurological intensive care unit (neuro-ICU). Although the consensus white paper suggests that a minimum of 5 min of data are needed for assessing dynamic cerebral autoregulation with transfer function analysis (TFA), it remains unknown if the length of these data is valid for patients in the neuro-ICU, of whom are notably different than the general populations. We aimed to investigate the effect of data length using transcranial Doppler ultrasound combined with invasive blood pressure measurement for the assessment of dynamic cerebral autoregulation in patients in the neuro-ICU. METHODS: Twenty patients with various clinical conditions (severe acute encephalitis, ischemic stroke, subarachnoid hemorrhage, brain injury, cerebrovascular intervention operation, cerebral hemorrhage, intracranial space-occupying lesion, and toxic encephalopathy) were recruited for this study. Continuous invasive blood pressure, with a pressure catheter placed at the radial artery, and bilateral continuous cerebral blood flow velocity with transcranial Doppler ultrasound were simultaneously recorded for a length of 10 min for each patient. TFA was applied to derive phase shift, gain, and coherence function at all frequency bands from the first 2, 3, 4, 5, 6, 7, 8, 9, and 10 min of the 10-min recordings in each patient on both hemispheres. The variability in the autoregulatory parameters in each hemisphere was investigated by repeated measures analysis of variance. RESULTS: Forty-one recordings (82 hemispheres) were included in the study. According to the critical values of coherence provided by the Cerebral Autoregulation Research Network white paper, acceptable rates for the data were 100% with a length ≥ 7 min. The final analysis included 68 hemispheres. The effects of data length on trends in phase shift in the very low frequency (VLF) band (F1.801,120.669 = 6.321, P = 0.003), in the LF band (F1.274,85.343 = 4.290, P = 0.032), and in the HF band (F1.391,93.189 = 3.868, P = 0.039) were significant for 3-7 min, for 4-7 min, and for 5-8 min, respectively. Effects were also significant on the gain in the VLF band (F1.927,129.134 = 3.215, P = 0.045) for 2-8 min and on the coherence function in all frequency bands (VLF F2.846,190.671 = 90.247, P < 0.001, LF F2.515,168.492 = 55.770, P < 0.001, HF F2.411, 161.542 = 33.833, P < 0.001) for 2-10 min. CONCLUSIONS: Considering the acceptable rates for the data and the variation in the TFA variables (phase shift and gain), we recommend recording data for a minimum length of 7 min for TFA in patients in the neuro-ICU.


Assuntos
Circulação Cerebrovascular , Ultrassonografia Doppler Transcraniana , Velocidade do Fluxo Sanguíneo/fisiologia , Pressão Sanguínea/fisiologia , Circulação Cerebrovascular/fisiologia , Homeostase/fisiologia , Humanos , Unidades de Terapia Intensiva
15.
J Hazard Mater ; 424(Pt A): 127346, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-34601409

RESUMO

Sludge pyrolysis carbonization has shown potential to convert sludge biomass into multifunctional carbon materials. However, ecological risks of dissolved organic matters (DOMs) with obscure molecular characteristics retaining in sludge-based carbons (SBCs) have received little attention. This study investigated the impact of pyrolysis temperatures on the molecular conversion and biotoxicity effects of DOMs in SBCs. The results revealed that DOMs in SBCs300-400 were mainly derived from depolymerization of biopolymers and the polycondensation and cyclization of small intermediate molecules, which mainly consisted of aromatic CHON compounds with 1-3 N atoms, featuring high unsaturation and molecular weights. High-temperature pyrolysis (500-800 °C) promoted the decomposition and ring-opening of aromatic CHON compounds into saturated aliphatic CHO compounds with 2-4 O atoms in SBCs500-800. Noteworthily, SBCs300-400-derived DOMs showed relatively strong biotoxicity on the growth and development of wild-type zebrafish embryos, pakchoi seeds, and Vibrio qinghaiensis Q67, which was significantly related to aromatic amines, phenols, and heterocyclic-N compounds in DOMs of SBCs300-400. SBCs500-800-derived DOMs were mainly straight-chain fatty acids and showed no observable acute biotoxicity. This study highlights the negative impact of DOMs in SBCs on the ecological environment, and provides the theoretical basis for controlling toxic byproducts in sludge pyrolysis process.


Assuntos
Pirólise , Esgotos , Animais , Carbono , Temperatura , Peixe-Zebra
16.
Psychophysiology ; 59(1): e13949, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34587299

RESUMO

The incidence of depression is increasing, especially in the young adult population. Impaired cognitive function is one of the characteristics of depression, which may be related to impaired cerebral autoregulation (CA). We investigated the characteristics of CA in young adults with mild depression, as well as its validity for identifying patients with depression. Patients (aged 18-35 years) with Hamilton Depression Rating Scale (HAMD) scores ranging from 8 to 17 and a first episode of mild depression were enrolled in this study. Healthy volunteers were recruited as controls. Noninvasive continuous arterial blood pressure and bilateral middle cerebral artery blood flow velocity were simultaneously recorded from each subject. Transfer function analysis was applied to derive phase difference, gain, coherence and rate of recovery for the assessment of CA. Forty-three patients and 43 healthy controls were enrolled. Phase difference values were significantly compromised in young adults with mild depression and were negatively correlated with HAMD scores. Rate of recovery values estimated from depressed patients was significantly lower. The validity in identifying patients with depression was favorable for the phase difference. The cutoff phase difference value was 29.66. Our findings suggest that dynamic CA was impaired in young patients with mild depression and negatively correlated with HAMD scores. CA represented by phase difference can be used as an objective auxiliary examination of depression, and has clinical diagnostic value for the early identification of patients with depression.


Assuntos
Velocidade do Fluxo Sanguíneo/fisiologia , Depressão/psicologia , Homeostase/fisiologia , Artéria Cerebral Média/fisiopatologia , Adulto , Pressão Sanguínea/fisiologia , Escalas de Graduação Psiquiátrica Breve , Feminino , Humanos , Masculino , Estudos Prospectivos , Ultrassonografia Doppler Transcraniana
17.
Sci Total Environ ; 805: 150353, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34818788

RESUMO

Recovering high value-added resources from waste activated sludge (WAS) is a potential way for the sustainable wastewater treatment. In this study, hydrothermal treatment at 180 °C was used to simultaneously improve sludge dewaterability and recover sludge organic matters (SOMs). The recovered SOMs were subsequently employed as precursors to prepare nitrogen-doped porous carbon nanosheets via a facile stepwise synthesis method. The as-prepared optimal carbon (AP-SOM800) was characterized with an ultrahigh specific surface area (3473 m2/g), appropriate porosity (1.77 cm3/g), and abundant heteroatoms (1.47% N and 7.44% O). AP-SOM800 exhibited a high specific capacitance (409 F/g at 0.25 A/g), low resistance (0.52 Ω), and superior cyclic stability (only 9.09% loss after 10,000 cycles) in 6 M KOH aqueous electrolyte. Furthermore, AP-SOM800 demonstrated an extraordinary adsorption capacity (1528 mg/g for methyl orange (MO) and 1265 mg/g for tetracycline (TC)) that can be maintained (˃ 1200 mg/g) over a wide range of pH conditions. Specifically, 80.97% of MO and 66.67% of TC were rapidly absorbed through AP-SOM800 within 10 min, and 90.27% of MO and 81.24% of TC were eventually removed from wastewater after 60 min. The adsorption processes fit closely with the pseudo-second-order kinetic (R2 > 0.999) and Langmuir models (R2 > 0.914), revealing that the adsorption processes were dominated by a monolayer chemical adsorption reaction. This study suggests that high value-added materials can be obtained from the WAS through improving and extending the traditional sludge treatment processes, which will enrich the technical options available for future sustainable sludge treatment and disposal.


Assuntos
Esgotos , Purificação da Água , Adsorção , Carbono , Nitrogênio , Águas Residuárias
18.
Talanta ; 238(Pt 2): 123062, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34801915

RESUMO

We present a vacuum ultraviolet (VUV) lamp-based photoionization time-of-flight (TOF) mass spectrometer coupled with a capillary inlet and an aerodynamic lens to online analyze the chemical compositions of the gas- and particulate-phase of cigarette smoke of a heated tobacco product (HTP). Both phase compositions of the fresh cigarette smoke, without dilution and pretreatment, are softly photoionized and their mass spectra are measured with a time resolution of 1 s. It is shown that the gas-phase compositions with low mass are volatile organic compounds (VOCs), and the particulate-phase compositions are also clearly identified and cover the full mass range of the mass spectrometer. The time- or puff-by-puff resolved dynamic data are obtained for each species and provide abundant information to unravel the chemistry of the HTP smoke. In addition, the present results show that besides thermal vaporization, a couple of chemical reactions including pyrolysis and degradation have also occurred in the HTP smoking process, although its operation temperature is less than 350 °C. Even if not done here, this study paves the way to analyze the gas- and particulate-phase chemical compositions of a complex system in real time, like the cigarette smoke presented here, by using advanced soft ionization mass spectrometry.


Assuntos
Fumaça , Produtos do Tabaco , Espectrometria de Massas , Fumaça/análise , Fumar , Tabaco , Vácuo
19.
Phys Rev Lett ; 127(18): 180502, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34767431

RESUMO

We report phase-programmable Gaussian boson sampling (GBS) which produces up to 113 photon detection events out of a 144-mode photonic circuit. A new high-brightness and scalable quantum light source is developed, exploring the idea of stimulated emission of squeezed photons, which has simultaneously near-unity purity and efficiency. This GBS is programmable by tuning the phase of the input squeezed states. The obtained samples are efficiently validated by inferring from computationally friendly subsystems, which rules out hypotheses including distinguishable photons and thermal states. We show that our GBS experiment passes a nonclassicality test based on inequality constraints, and we reveal nontrivial genuine high-order correlations in the GBS samples, which are evidence of robustness against possible classical simulation schemes. This photonic quantum computer, Jiuzhang 2.0, yields a Hilbert space dimension up to ∼10^{43}, and a sampling rate ∼10^{24} faster than using brute-force simulation on classical supercomputers.

20.
Aging (Albany NY) ; 13(21): 24171-24191, 2021 11 05.
Artigo em Inglês | MEDLINE | ID: mdl-34740994

RESUMO

Genomic instability (GIN) is pivotal in regulating tumor drug resistance, which blocked the treatment of triple negative breast cancer (TNBC). Although recent studies implied that non-coding RNA (ncRNA)-mediated autophagy abolishment promoted tumorigenesis by up-regulation of GIN, autophagy was known as a risk factor in tumor drug resistance. However, previous study also pointed that up-regulation of autophagy promoted GIN. Therefore, the relationship between autophagy and GIN is not clear, and more work is needed. And, if an ncRNA is identified to be a co-regulator of autophagy and GIN, it will be a potential therapy target of chemotherapy resistance in TNBC. In our study, we recognized both autophagy-GIN-associated microRNA (mi-26a-5p) by big data analysis, which was prognosis-correlated in breast cancer. Next, we identified the up-stream regulators (long non-coding RNA, lncRNA) and down-stream targets of miR-26a-5p by bioinformatics analysis (online public databases). Finally, we established lncRNA OTUD6B-AS1/miR-26a-5p/MTDH signaling pathway, and verified their functions by cytological, molecular biological and zoological experiments. In general, our study found (1) miR-26a-5p was a protective factor of breast cancer, while OTUD6B-AS1 and MTDH were risk factors; (2) OTUD6B-AS1 was the up-stream regulator of miR-26a-5p verified by luciferase; (3) up-regulation of miR-26a-5p and down-regulation of MTDH promoted cellular cytotoxicity of paclitaxel (PTX) in vitro and in vivo. (4) down-regulation of miR-26a-5p, overexpression of MTDH and OTUD6B-AS1 promoted autophagy and DNA damage; (5) up-regulation of OTUD6B-AS1 and MTDH inhibited DNA damage response (DDR) by inhibiting the phosphorylated activation of RAD51, ATR and ATM.


Assuntos
Resistencia a Medicamentos Antineoplásicos/genética , MicroRNAs/genética , Paclitaxel/farmacologia , RNA Longo não Codificante/genética , Neoplasias de Mama Triplo Negativas , Animais , Antineoplásicos/farmacologia , Autofagia/genética , Linhagem Celular Tumoral , Feminino , Instabilidade Genômica/genética , Humanos , Proteínas de Membrana/genética , Camundongos Nus , Proteínas de Ligação a RNA/genética , Neoplasias de Mama Triplo Negativas/genética , Neoplasias de Mama Triplo Negativas/metabolismo
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