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1.
J Colloid Interface Sci ; 605: 231-240, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34329976

RESUMO

Flexible electrode plays a key role in flexible energy storage devices. The SnTe/C nanofibers membrane (SnTe/CNFM) with excellent mechanical flexibility has been successfully synthesized for the first time through electrospinning, and it demonstrates outstanding electrochemical performance as free-standing anode for lithium/sodium-ion batteries. The SnTe/CNFM electrode delivers a discharge capacity of 526.7 mAh g-1 at 1000 mA g-1 after 1000 cycles in lithium-ion half-cells and a discharge capacity of 236.5 mAh g-1 at 500 mA g-1 after 80 cycles in lithium-ion full-cells with a LiFePO4 cathode. Not only that, it shows a discharge capacity of 182.7 mAh g-1 at 200 mA g-1 after 200 cycles in sodium-ion half-cells and a high discharge capacity of 207.0 mAh g-1 at 500 mA g-1 after 50 cycles in sodium-ion full-cells with a Na0.44MnO2 cathode. Moreover, the prepared SnTe/CNFM exhibits good mechanical flexibility. The SnTe/CNFM can still return to its original state without any breakage after bending, curling, folding and kneading. These results indicate that SnTe/CNFM is expected to become one of the promising free-standing anodes for lithium/sodium-ion batteries.

2.
Dev Comp Immunol ; 127: 104309, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34748796

RESUMO

Insect C-type lectins (CTLs) play crucial roles in modulating the humoral and cellular immune responses. In the domesticated silkworm Bombyx mori L., BmCTL10 gene encodes an immulectin containing two carbohydrate recognition domains (CRDs). The phylogenetic analysis showed that BmCTL10 didn't cluster with other immulectin homologs in B. mori. BmCTL10 was mainly expressed in second to fifth instar larvae, wandering stage larvae, prepupa, and adults. In naïve fifth instar larvae, BmCTL10 was predominantly expressed in the fat body and epidermis. In second instar larvae, the topical application of Beauveria bassiana by immersion caused down-regulation of BmCTL10. The intra-hemocoel injection of E. coli, S. aureus, B. bassiana, and 20-hydroxyecdysone in fifth instar larvae caused tissue and time-specific inductions. The recombinant protein (rBmCTL10) can bind to larval hemocytes and various pathogen-associated molecular patterns to enhance hemocyte-mediated nodulation, phagocytosis, and encapsulation. rBmCTL10 caused significant upregulation of most antimicrobial peptides and nitric oxide synthase 1 in hemocytes in vivo. Yeast two-hybrid demonstrated that integrin ß3 and ß4 subunits can interact with BmCTL10. Furthermore, only CRD2 can interact with the ß3, while both CRD1 and CRD2 can interact with the ß4. Taken together, this study showed that BmCTL10 has multiple functions in the innate immune responses of B. mori and two integrin ß subunits are their potential receptors.

3.
Sci Total Environ ; 806(Pt 1): 150370, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34562760

RESUMO

Predicting the migration behavior of volatile organic compounds (VOCs) vapor is essential for the remediation of subsurface contamination such as soil vapor extraction. Previous analytical prediction models of VOCs migration are mostly limited to constant-concentration nonpoint sources in homogeneous soil. Thus, this study presents a novel analytical model for two-dimensional transport of VOCs vapor subjected to multiple time-dependent point sources involving transient diffusion, sorption and degradation in layered unsaturated soils. Two representative time-dependent sources, i.e., an instantaneous source and a finite pulse source, are used to describe the short-term and long-term leakage. Results reveal that soil heterogeneity can cause pollution accumulation, especially in low-diffusivity capillary fringe. The assumption of an equivalent plane source from multiple point sources would significantly overestimate the vapor concentration and the contaminated range. The previous single point source model is no longer inapplicable when the relative distance and/or the release interval between sources is small, giving a strong interaction between multiple sources. Moreover, a faster vapor degradation rate or a higher groundwater level will reduce the area of vapor plume linearly. Hence, close attention should be paid to the time-variation characteristics of multiple sources, the vapor degradation and the groundwater level fluctuation in practice to facilitate soil remediation. The proposed model is a promising tool for addressing the above issue.


Assuntos
Água Subterrânea , Poluentes do Solo , Compostos Orgânicos Voláteis , Poluição Ambiental , Gases , Solo , Poluentes do Solo/análise
4.
J Colloid Interface Sci ; 607(Pt 2): 1353-1361, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34583040

RESUMO

Edge sites and interlayer space of NiFe layered double hydroxides (LDHs) play an important role in water oxidation. However, the combined effect of interlayer expansion and partial exfoliation on the catalytic activity is yet to be investigated. Herein, scalable synthesis of partially exfoliated citrate-intercalated NiFe LDHs with tunable interlayer space have been achieved. The effect of citrate concentration on the phase, morphology, surface elemental composition, electronic states of surface metals, and electrochemical properties are comprehensively studied. The unique structure results in improved intrinsic catalytic activity and abundant active edge sites for oxygen evolution reaction. The optimal NiFe LDHs show an overpotential of 225 mV at 10 mA cm-2, which is much smaller than that (∼305 mV) of the single-layer NiFe LDH nanosheets reported in the literature. The high catalytic activity can be mainly attributed to the combined effect between the enlarged interlayer space and the partial exfoliation/nanosheet thickness. That is, the interlayer space is related to the reaction kinetics/mechanism, while the degree of exfoliation affects the magnitude of the current density at a certain potential.

5.
Anal Chim Acta ; 1189: 339224, 2022 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-34815036

RESUMO

Psoralen ultraviolet A (PUVA) therapy has thrived as a promising treatment for psoriasis. However, overdose of PUVA treatment will cause side-effects, such as melanoma formation. And these side-effects are often ignored during PUVA therapy. Hence, in situ monitoring therapeutic response of PUVA therapy is important to minimize side-effects. Aberrant expression of tyrosinase (TYR) has been proved to be associated with melanoma, indicating that TYR is a potential target for evaluation of PUVA therapy. Herein, we reported a strategy for in situ monitoring TYR activity during PUVA therapy by using a cell-array chip-based SERS platform. The cell-array chip was used to simulate cell survival environment for cell culture. Capture of single cells and living cell analysis were realized in the isolated microchambers. An enzyme-induced core-shell self-assembly substrate was used to evaluate TYR activity in living cells during PUVA therapy. The gold nanoparticle modified with a SERS reporter, 4-mercaptobenzonitrile (4-MBN), was used as the core. In the presence of oxygen and TYR, hydroxylation of l-tyrosine occurred, leading to the reduction of silver ion on the surface of gold cores. The growth of silver shells was accompanied by the increased SERS intensity of the reporter, which is related directly to TYR activity. The detection limit for TYR activity is 0.45 U/mL. Upregulation of TYR activity was successfully monitored after PUVA therapy. Notably, real-time and in situ information of therapeutic response can be obtained through monitoring PUVA therapy by using a cell-array chip-based SERS platform, which has great potential to guide the clinical application of PUVA therapy.


Assuntos
Ouro , Nanopartículas Metálicas , Terapia PUVA , Prata , Análise Espectral Raman
6.
Food Chem ; 370: 131276, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34662790

RESUMO

This study presented an effective and sensitive SERS substrate for rapid detection of thiabendazole (TBZ) in fruit samples. A core-shell gold/silver nanorod (Au@Ag NRs) has been synthesized as a bimetallic SERS-active substrate. The obtained substrate showed an excellent SERS effect because of the tunable plasmon resonance of Au NRs, the significantly enhanced effect of silver, and the bimetallic synergistic effect of Au@Ag NRs. Under optimal conditions, the substrate was used to detect TBZ in fresh apple juice and peach juice with limits of detection of 0.032 and 0.034 ppm respectively. In addition, the recovery rate was within a satisfactory range of 95-101%, indicating that the Au@Ag NRs substrate could be a SERS detection platform for fruit pesticides residues with great development potential.


Assuntos
Nanopartículas Metálicas , Nanotubos , Sucos de Frutas e Vegetais , Ouro , Análise Espectral Raman , Tiabendazol/análise
7.
Chemosphere ; 286(Pt 3): 131963, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34426263

RESUMO

BACKGROUND: Exposure to air pollution has been linked with altered immune function in adults, but little is known about its effects on early life. This study aimed to investigate the effects of exposure to air pollution during prenatal and postnatal windows on cell-mediated immune function in preschoolers. METHODS: Pre-school aged children (2.9 ± 0.5 y old, n = 391) were recruited from a mother-child cohort study in Wuhan, China. We used a spatial-temporal land use regression (LUR) model to estimate exposures of particulate matter with aerodynamic diameters ≤2.5 µm (PM2.5) and ≤10 µm (PM10), and nitrogen dioxide (NO2) during the specific trimesters of pregnancy and the first two postnatal years. We measured peripheral blood T lymphocyte subsets and plasma cytokines as indicators of cellular immune function. We used multiple informant models to examine the associations of prenatal and postnatal exposures to air pollution with cell-mediated immune function. RESULTS: Prenatal exposures to PM2.5, PM10, and NO2 during early pregnancy were negatively associated with %CD3+ and %CD3+CD8+ cells, and during late pregnancy were positively associated with %CD3+ cells. Postnatal exposures to these air pollutants during 1-y or 2-y childhood were positively associated with IL-4, IL-5, IL-6, and TNF-α. We also observed that the associations of prenatal or postnatal air pollution exposures with cellular immune responses varied by child's sex. CONCLUSIONS: Our results suggest that exposure to air pollution during different critical windows of early life may differentially alter cellular immune responses, and these effects appear to be sex-specific.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Poluentes Atmosféricos/toxicidade , Poluição do Ar/efeitos adversos , Poluição do Ar/análise , Criança , Pré-Escolar , Estudos de Coortes , Exposição Ambiental/efeitos adversos , Exposição Ambiental/análise , Feminino , Humanos , Imunidade Celular , Masculino , Dióxido de Nitrogênio/análise , Dióxido de Nitrogênio/toxicidade , Material Particulado/análise , Material Particulado/toxicidade , Gravidez
8.
J Hazard Mater ; 423(Pt B): 127106, 2022 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-34536848

RESUMO

Developing P-efficient plants helps improve P uptake from soils with low-available P and reduce environmental damage by P runoff. Here, we investigated a novel root-specific phytase PvPHY1 from As-hyperaccumulator Pteris vittata, which can efficiently utilize phytate, a recalcitrant organic phosphorus in soil. Unlike other plants, expression of PvPHY1 in P. vittata was greater in the roots than the fronds. A pure phytase with considerable activity was obtained via prokaryotic expression. Expressing PvPHY1 in tobacco (PvPHY1-Ex) enhanced its growth (2.8 to 3.5-3.9 g per plant) and increased its P accumulation by 10-50% under low- and adequate-P conditions. Further, PvPHY1-Ex tobacco showed 25-32% lower intracellular phytate and 30-56% higher inorganic P in the roots, likely due to phytase-mediated hydrolysis of phytate. Decrease of phytate levels up-regulated phosphate transporter genes (NbPht1;1, NbPht1;2 and NbPht1;6), leading to greater P and As uptake. However, As translocation to the shoots was low, probably due to competition from increased inorganic P via phytate hydrolysis. As such, PvPHY1 facilitated P uptake from soils and phytate hydrolysis in plants, thereby promoting tobacco growth. Overall, PvPHY1 from P. vittata helps better understand the novel phytase to increase soil P utilization efficiency, thereby reducing P fertilizer requirements for crop production.

9.
J Ethnopharmacol ; 283: 114670, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34653522

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Fritillariae thunbergii Miq (FTM)exhibit versatile biological activities including the significant antitussive and expectorant activities. As a herbal medicine, the therapeutic effects of FTM may be expressed by multi-components which have complicated integration effects on multi-targets. With the time going, the different processing methods of FTM has been changed a lot. Thus,the study described the effect of processing methods to FTM and its quality. MATERIAL AND METHOD: Studies were undertaken by using UHPLC-LTQ Orbitrap MS and pharmacodynamic models. All reagents were involved of analytical grade. While a HPLC-ELSD's method has been developed and validated, a certified Quality System is conformed to ICH requirements. The experimental animals followed the animal welfare guidelines. AIM OF THE STUDY: We aimed to found the differences after the different processing methods of FTM, and to demonstrate the changes could be selected as quality control indicators, and established a method for simultaneous determination of these for quality control. RESULTS: we have previously found two new steroidal alkaloids: zhebeininoside and imperialine-3-ß-D-glucoside from the different processing methods of FTM, which is the difference between the different processing methods of FTM, mainly on the steroidal alkaloids. The activity analysis of zhebeininoside, imperialine-3-ß-D-glucoside, verticine and verticinone showed that the mouse model of cough expectorant has antitussive effect. The positive drug selected was dextromethorphan syrup. The positive group showed biological activity, but the blank group showed nothing. The model group showed illness which means that the model was effective. There are two ways of the mechanism of action of the expectorant action which can make sputum thin, reduce its viscosity, and be easy to cough up, or can accelerate the movement of mucous cilia in the respiratory tract and promote the discharge of sputum. In our study, the content of phenol red was significantly reduced in the administration group. CONCLUSIONS: To sum up, our results suggest that zhebeininoside and other three components cloud be selected as quality control indicators, and a method for simultaneous determination of zhebeininoside and other three components was established for quality control.

10.
J Ethnopharmacol ; 283: 114747, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34656667

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The Plant Xanthoceras sorbifolium Bunge (X. sorbifolia) has a long history of medicinal use as a traditional Chinese herbal medicine to deal with sterilizing, killing sperm, stabilizing capillary, hemostasis, lowering cholesterol, rheumatism, and pediatric enuresis. Additionally, X. sorbifolia is an oil crop for the production of edible oil due to the health-promotion effect. In recent years, X. sorbifolia has attracted worldwide attention as an important economic crop with low investment and high-income potential. AIM OF THE REVIEW: This review aims to provide a comprehensive appraisal of X. sorbifolia, including the traditional uses, nutrients, phytochemical data, biological activities, and current applications. The natural compounds of X. sorbifolia and potential utilization in pharmacology are highlighted. The aim of this review is to inspire the research enthusiasm to X. sorbifolia and promote the comprehensive utilization of X. sorbifolia. MATERIALS AND METHODS: The research information of X. sorbifolia was collected via Elsevier, American Chemical Society (ACS), PubMed, Web of Science, China National Knowledge Infrastructure (CNKI), Baidu scholar, and Google scholar. Additionally, some information was collected from Ph.D. and Master's dissertations, as well as local books. RESULTS: The identification of approximately 195 major phytochemical compounds from different parts of X. sorbifolia is presented in this review, including triterpenoids, flavonoids, phenolic acids, coumarins, lignans, meroterpenoids, monoterpene, alkaloids, and sterol. Among them, triterpenoids, flavonoids, and phenolic acids are the major compounds. Extracts from X. sorbifolia exhibited a wide range of biological activities, such as antioxidant, antibacterial, anti-tumor, anti-neuroinflammatory, anti-adipogenesis, anti-obesity, anti-HIV, gastroprotective, immunoregulatory, and anti-inflammatory activities. CONCLUSIONS: Modern pharmacological studies have been well supported and clarified the traditional medicinal uses of X. sorbifolia, which brought a promising prospect for the pharmaceutical value of this plant. However, the related mechanisms between the structure and pharmacological effects were seldom reported. Also, at present, effective and in-depth research on X. sorbifolia is still relatively lacking. Moreover, there is little research on toxicological experiments. Further clinical trials should also be performed to accelerate the drug research and development.

12.
J Cancer Res Ther ; 17(5): 1269-1274, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34850777

RESUMO

Objectives: The objective of the study was to assess the clinical efficacy of computed tomography (CT)-guided cryoablation as a means to treat adrenal metastasis (AM) secondary to lung cancer. Materials and Methods: This study was a single-center retrospective study that analyzed 39 consecutive patients with AM secondary to lung cancer who underwent CT-guided cryoablation in our center. The rates of complete ablation, local recurrence, local recurrence-free survival (RFS), and overall survival (OS) were analyzed. Results: The rates of primary and secondary complete ablation were 94.9% and 100%, respectively, and none of the patients suffered from a hypertensive crisis associated with the treatment. Over the follow-up period, 20.5% of the patients experienced local recurrence, and the median RFS duration was 26 months. The cumulative 1-, 3-, and 5-year local RFS rates in this study were 84.6%, 51.3%, and 5.9%, respectively. Extra-adrenal gland metastases were detected in five patients. Over the course of follow-up, 26 patients died. The mean OS duration was 34 months with cumulative 1-, 3-, and 5-year OS rates of 89.7%, 53.4%, and 8.3%, respectively. Advanced age (P = 0.001), primary adenocarcinoma (P = 0.006), other primary lung cancers (P = 0.038), and primary Stage III lung cancers (P = 0.007) were all found to be independent predictive factors of poor OS in these patients. Conclusion: CT-guided cryoablation can be safely and effectively used to control AM secondary to lung cancer, and patients with AM secondary to lung squamous cell carcinoma may be best suited for this form of treatment.

13.
Brief Bioinform ; 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34727569

RESUMO

Predicting the response of a cancer cell line to a therapeutic drug is an important topic in modern oncology that can help personalized treatment for cancers. Although numerous machine learning methods have been developed for cancer drug response (CDR) prediction, integrating diverse information about cancer cell lines, drugs and their known responses still remains a great challenge. In this paper, we propose a graph neural network method with contrastive learning for CDR prediction. GraphCDR constructs a graph neural network based on multi-omics profiles of cancer cell lines, the chemical structure of drugs and known cancer cell line-drug responses for CDR prediction, while a contrastive learning task is presented as a regularizer within a multi-task learning paradigm to enhance the generalization ability. In the computational experiments, GraphCDR outperforms state-of-the-art methods under different experimental configurations, and the ablation study reveals the key components of GraphCDR: biological features, known cancer cell line-drug responses and contrastive learning are important for the high-accuracy CDR prediction. The experimental analyses imply the predictive power of GraphCDR and its potential value in guiding anti-cancer drug selection.

15.
Int Ophthalmol ; 2021 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-34743256

RESUMO

PURPOSE: To introduce and evaluate a modified therapeutic strategy for idiopathic macular holes (IMH). METHODS: A retrospective review of patients with diagnosis of IMH from July 2016 to January 2020 at Peking Union Medical College Hospital. These patients were managed strictly according to our therapeutic strategy. Their comprehensive clinical data were collected and analyzed. RESULTS: 209 eyes suffering stage II to IV IMH were identified. For stage II IMH, the spontaneous closure rate was 8.9%, the initial success rate of intravitreal injections (IVI) of expansile gas and pars plana vitrectomy (PPV) + internal limiting membrane peeling (ILMP) + air tamponade was 84.2% and 100%, respectively. The initial success rate of PPV + ILMP + air tamponade for stage III and stage IV IMH was 89.8% and 86.4%, respectively. Following our intervention strategy, stage II IMH achieved a final IMH closure rate of 100%, stage III of 99% and stage IV of 97%. The final best corrected visual acuity was significantly improved (P < 0.05). Sitting position air-fluid (A-F) exchange alone successfully induced IMH closure in 7/19 eyes that did not achieve IMH closure by initial PPV. For three refractory cases that failed additional PPV + ILM stuffing, intraoperative OCT assisted PPV + sub-retinal BSS injection successfully induced the IMH closure. As the remaining three unclosed IMH cases were dry and stable, no more interventions were conducted. CONCLUSION: The general IMH closure rate based on our therapeutic strategy was satisfactory with a favorable prognosis. IVI expansile gas and sitting position A-F exchange were effective and highly cost-effective under certain circumstances.

16.
Autophagy ; : 1-14, 2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34747299

RESUMO

Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging negatively stranded enveloped RNA bunyavirus that causes SFTS with a high case fatality rate of up to 30%. Macroautophagy/autophagy is an evolutionarily conserved process involved in the maintenance of host homeostasis, which exhibits anti-viral or pro-viral responses in reaction to different viral challenges. However, the interaction between the bunyavirus SFTSV and the autophagic process is still largely unclear. By establishing various autophagy-deficient cell lines, we found that SFTSV triggered RB1CC1/FIP200-BECN1-ATG5-dependent classical autophagy flux. SFTSV nucleoprotein induced BECN1-dependent autophagy by disrupting the BECN1-BCL2 association. Importantly, SFTSV utilized autophagy for the viral life cycle, which not only assembled in autophagosomes derived from the ERGIC and Golgi complex, but also utilized autophagic vesicles for exocytosis. Taken together, our results suggest a novel virus-autophagy interaction model in which bunyavirus SFTSV induces classical autophagy flux for viral assembly and egress processes, suggesting that autophagy inhibition may be a novel therapy for treating or releasing SFTS.

17.
J Diabetes Res ; 2021: 4027380, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34746318

RESUMO

Aims: Phthalates, which are recognized environmental endocrine-disrupting chemicals, are associated with thyroid hormone disruption. We aimed to evaluate the relationship of phthalate metabolites with thyroid function and thyroid homeostasis parameters in type 2 diabetes and to explore whether thyroid autoimmunity status and metformin, the most common antidiabetic drug, may influence such associations. Methods: Concurrent urine and blood samples were collected from 639 participants with type 2 diabetes in the METAL (Environmental Pollutant Exposure and Metabolic Diseases in Shanghai) study. We measured urinary concentrations of thirteen phthalate metabolites along with serum levels of thyroid-stimulating hormone (TSH), total T4 and T3, free T4 (FT4) and T3 (FT3), thyroid peroxidase antibody (TPOAb), and thyroglobulin antibody (TgAb). Four parameters of thyroid homeostasis, including the sum activity of step-up deiodinases (SPINA-GD), thyroid secretory capacity (SPINA-GT), Jostel's TSH index (TSHI), and thyrotroph thyroid hormone resistance index (TTSI), were also calculated. Results: Among all participants, after full adjustment, multivariable regression analysis showed that some urine phthalate metabolites were negatively associated with TSH, TSHI, and TTSI levels and positively associated with FT3, T3, SPINA-GD, and SPINA-GT levels. None of the urine phthalate metabolites exhibited a significant association with thyroid autoimmunity. The associations of phthalate metabolites with thyroid function and thyroid homeostasis parameters differed based on thyroid autoantibody and metformin treatment status. Conclusions: Urinary phthalate metabolites may be associated with thyroid function and thyroid homeostasis parameters among participants with type 2 diabetes. Furthermore, our present study suggested that thyroid autoantibody status and metformin treatment status are potential mediators of such associations.

18.
AIDS Res Ther ; 18(1): 82, 2021 11 02.
Artigo em Inglês | MEDLINE | ID: mdl-34727929

RESUMO

BACKGROUND: To evaluate the effect of reconstruction and repair, using a mini-plate and bone graft for HIV -positive patients with giant cell tumor of long bone. METHODS: We conducted a retrospective analysis of 12 HIV positive patients with giant cell tumor of long bone. A non-HIV-positive cohort of patients, matched for age, sex, and disease type, was selected as the control group. From June 2012 to August 2020, curettage by ultrasonic scalpel was performed in all patients, combined with min- plate and bone graft treatment. All patients were followed- up for 18 to 60 months. Limb function was evaluated, using the MSTS93 scoring system, and any examples of postoperative recurrence, distant metastasis, complications, MSTS93 score, and fracture prognosis were recorded. RESULTS: The mean age of HIV group was 43.5 years. The ratio of men to women was 11: 1. In all cases the histopathological diagnosis was clear, except the patients with primary malignant giant cell tumor of bone, including five, three, two, and two cases in the proximal tibia, distal femur, distal tibia, and talus, respectively. Following their surgery, all patients were followed up with an average of 31.24 ± 11.84 months. No local recurrence or pulmonary metastases were observed. Post-surgery, all the 12 patients showed good bone morphologic repair and reconstruction, good bone healing, good joint function, and no pathological fractures around their lesion. In the HIV group, one case of giant cell tumor in the proximal tibia showed mild articular surface collapse and mild valgus deformity of the knee joint but retained good joint function. The MSTS scores of excellent or good in the two groups comprised 83.3%, thus, there was no significant difference between them (P > 0.05). Compared with preoperatively, the MSTS scores in the HIV group were significantly improved, ranging from 7 to 11 points preoperatively to 24 to 27 points postoperatively; this difference was statistically significant (P < 0.05). CONCLUSION: Reconstruction and repair, using a mini-plate and bone graft for HIV -positive patients with giant cell tumor of long bone can achieve satisfactory results. The mini- plate requires little space and is flexible during reconstruction and fixation, significantly reducing complications such as surgical site infection, as well as preserving joint function and avoiding amputation; therefore, it is a safe and effective treatment method.


Assuntos
Neoplasias Ósseas , Tumores de Células Gigantes , Infecções por HIV , Adulto , Neoplasias Ósseas/cirurgia , Feminino , Humanos , Masculino , Recidiva Local de Neoplasia , Estudos Retrospectivos , Resultado do Tratamento
19.
BMC Public Health ; 21(1): 2022, 2021 11 06.
Artigo em Inglês | MEDLINE | ID: mdl-34742268

RESUMO

BACKGROUND: Prevention and control (P&C) of Corona Virus Disease 2019 (COVID-19) is still a critical task in most countries and regions. However, there are many single evaluation indexes to assess the quality of COVID-19 P&C. It is necessary to synthesize the single evaluation indexes reasonably to obtain the overall evaluation results. METHODS: This study was divided into three steps. Step 1: In February 2020, the improved Delphi method was used to establish the quality evaluation indexes system for COVID-19 P&C. Step 2: in March 2020, the CRITIC method was used to adjust the Order Relation Analysis (G1) method to obtain the subjective and objective (S&O) combination weights. The comprehensive evaluation value was obtained using the weighted Efficacy Coefficient (EC) method, weighted TOPSIS method, weighted rank-sum ratio (RSR) method, and weighted Grey Relationship Analysis (GRA) method. Finally, the linear normalization method was used to synthesize the evaluation values of different evaluation methods. Step 3: From April 2020 to May 2021, this evaluation method was used to monitor and assess COVID-19 P&C quality in critical departments prospectively. The results were reported to the departments monthly. RESULT: A quality evaluation indexes system for COVID-19 P&C was established. Kendall's consistency test shows that the four evaluation method had good consistency (χ2 = 43.429, P<0.001, Kendall's consistency coefficient = 0.835). The Spearman correlation test showed that the correlation between the combined evaluation results and the original method was statistically significant(P < 0.001). According to the Mann-Kendall test, from March 2020 to May 2021, the mean value of COVID-19 P&C quality in all critical departments showed an upward trend (P < 0.01). CONCLUSIONS: The combined comprehensive evaluation method based on the S&O combined weight was more scientific and comprehensive than the single weighting and evaluation methods. In addition, monitoring and feedback of COVID-19 P&C quality were helpful for the improvement of P&C quality.


Assuntos
COVID-19 , Hospitais Gerais , Serviços de Saúde , Humanos , Estudos Prospectivos , SARS-CoV-2
20.
J Asian Nat Prod Res ; : 1-10, 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34762536

RESUMO

Two new prenylated xanthones, mangoxanthones A-B (1-2), together with four known compounds 3-6, were isolated from the ethanol extract of the pericarp of Garcinia mangostana. The structures of these compounds have been elucidated based on spectroscopic analysis. The analysis results of chiral HPLC revealed compounds 1 and 2 were scalemic mixtures respectively. All isolated compounds were biologically evaluated for their α-glucosidase and α-amylase inhibitory effects using in-vitro assays. Compound 1 showed moderate inhibitory activities against α-glucosidase and α-amylase with IC50 of 29.06 ± 1.86 and 22.74 ± 2.07 µM, respectively. Molecular docking predicted the binding sites of compound 1 to α-glucosidase and α-amylase. A preliminary structure-activity relationship was discussed.

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