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1.
J Nanosci Nanotechnol ; 20(2): 1013-1019, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383099

RESUMO

Nanomaterial-based flexible strain sensors have developed rapidly in recent years. Here, we propose a flexible strain sensor based on polydimethylsiloxane with carbon nanotubes (CNTs) and graphene quantum dots (GQDs). Different weight ratios of CNTs and GQDs were used as the sensitive units of the strain sensors. After analyzing the results of current-voltage curves and the strain effects of the sensors, we concluded that the introduction of GQDs played an important role in improving the sensitivity of the sensors. The gauge factor of the as-prepared strain sensors ranges from 0 to 841.42.

2.
J Pharm Biomed Anal ; 177: 112848, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31479998

RESUMO

Metabolic syndrome (MetS) is an important risk factor for type 2 diabetes, cardiovascular diseases and all-cause morbidity and mortality. Biomarkers can provide insight into the mechanism, facilitate early detection, and monitor progression of MetS and its response to therapeutic interventions. To identify potential biomarkers, we applied a non-targeted and targeted lipidomics method to characterize plasma metabolic profile in MetS patients. Metabolic profiling was performed on a non-target set (40 cases and 40 controls) on UHPLC-Q-TOF/MS and target set (80 MetS patients and 80 healthy controls) on UHPLC-Q-orbitrap MS. Using comprehensive screening and validation workflow, we identified a panel of three metabolites including PC(18:1/P-16:0), PC(o-22:3/22:3), PC(P-18:1/16:1). Our results indicated that the identified biomarkers may improve the risk prediction and provide a novel tool for monitoring of the progression of disease and response to treatment in MetS patients.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31483922

RESUMO

Metronidazole, a widely used drug for the treatment of infections with anaerobic and facultative anaerobic bacteria and protozoa, can frequently cause metronidazole-induced cutaneous adverse reactions (McADRs). The aim of the present study was to investigate the association between human leukocyte antigen (HLA) alleles and McADRs in a Chinese Han population. The frequency of HLA-B*24:02 carriers among the McADR patients was 73.3%, which was significantly higher than that of the population controls (32.16%, OR=5.80, 95% CI=[1.80-18.72], Pc=0.004) and of the metronidazole-tolerant patients (26.67%, OR=7.56, 95% CI=[2.02-28.35], Pc=0.004). Molecular docking showed that metronidazole and one of its major metabolites had the potential to bind in the HLA groove and that there was a relatively stable binding state of the HLA-B*24:02-metronidazole/the metabolite complex. The CDR3 repertoires of both T cell receptor (TCR)Vα and Vß of the patients showed a significantly skewed or an oligoclonal distribution. The TCRVß CDR3 of the patients shared a similar motif, 'CASSxxxxxxQxF'. The current study demonstrated that both the HLA-A*24:02 allele and TCR are involved in the pathogenesis of McADRs. This article is protected by copyright. All rights reserved.

4.
Genes (Basel) ; 10(9)2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31484412

RESUMO

Long non-coding RNAs (lncRNAs) are a class of RNAs with the length exceeding 200 base pairs (bps), which do not encode proteins, nevertheless, lncRNAs have many vital biological functions. A large number of novel transcripts were discovered as a result of the development of high-throughput sequencing technology. Under this circumstance, computational methods for lncRNA prediction are in great demand. In this paper, we consider global sequence features and propose a stacked ensemble learning-based method to predict lncRNAs from transcripts, abbreviated as PredLnc-GFStack. We extract the critical features from the candidate feature list using the genetic algorithm (GA) and then employ the stacked ensemble learning method to construct PredLnc-GFStack model. Computational experimental results show that PredLnc-GFStack outperforms several state-of-the-art methods for lncRNA prediction. Furthermore, PredLnc-GFStack demonstrates an outstanding ability for cross-species ncRNA prediction.

5.
Mol Med Rep ; 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31485634

RESUMO

Renal cell carcinoma (RCC) is the most common kidney malignancy, responsible for ~80% of all cases in adults. The pathogenesis of RCC is complex, involving alterations at both the genetic and epigenetic levels. Numerous signaling pathways, such as PI3K/Akt/mTOR and Wnt­ß­catenin have been demonstrated to be associated with the tumorigenesis and development of RCC. Long non­coding RNAs (lncRNAs) are functional RNA molecules involved in the initiation and progression of cancer, and investigating the effects of lncRNA could facilitate the development of novel treatments. The lncRNA regulator of reprogramming (ROR) is aberrantly expressed in a variety of tumors. However, its underlying mechanisms remain largely unknown. In the present study, ROR was found to be upregulated and microRNA (miR)­206 was found to be downregulated in RCC tissues and cells. Furthermore, the knockdown of ROR inhibited the proliferation, migration and invasion of RCC cells. It was found that ROR binds to miR­206, and that ROR­induced cell proliferation and metastasis were reversed by the overexpression of miR­206. In addition, the levels of miR­206 and ROR were negatively correlated in RCC tissues. Furthermore, the overexpression of miR­206 notably suppressed the proliferation, migration and invasion of RCC cells, and these effects were enhanced by the knockdown of vascular endothelial growth factor (VEGF); cell growth and metastasis induced by miR­206 inhibitors could be reversed by the knockdown of VEGF. In addition, the expression levels of miR­206 and VEGF were inversely correlated in RCC samples. In summary, the results of the present study revealed that ROR was upregulated in RCC tissues, which promoted tumor progression by regulating the miR­206/VEGF axis. The present findings provided a novel insight into the potential functions of ROR in RCC, and the ROR/miR­206/VEGF pathway may be a promising therapeutic target for the treatment of patients with RCC.

6.
Org Biomol Chem ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31486452

RESUMO

We report the first asymmetric iminium ion catalysis-enabled cascade cycloaddition reaction of bifunctional chromone-oxindole synthons and α,ß-unsaturated aldehydes. This allowed one quaternary and four tertiary contiguous stereogenic centers to be constructed in a single operation. A range of spirooxindole-hexahydroxanthone molecules are obtained with up to 62% yield, >20 : 1 dr and >99% ee. This reaction has not only provided a new approach for constructing hexahydroxanthone-fused scaffolds by utilizing asymmetric iminium ion catalysis, but also advanced the chemistry of diversity-oriented synthesis based on bifunctional chromone synthons.

7.
Orthop Surg ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31486589

RESUMO

Os acromiale is a developmental defect which results from the lack of an osseous union between the ossification centers of the acromion, leading to the fibrocartilaginous tissue connection. The prevalence of os acromiale is 1% to 15%, and is quite common in the African American population. Os acromiale in adults is easily diagnosed by symptoms and X-ray, particularly on the axillary view; however, the differential diagnosis of adolescents may require MRI or SPECT-CT. Generally, nonoperative therapy for symptomatic os acromiale should be started, including physiotherapy, nonsteroidal anti-inflammatory drugs, and injections. Surgical treatment is indicated after failed conservative treatment. In symptomatic patients with fixable acromiale, the tension band technique should be used to make the anterior aspect of the acromion elevated from the humerus head. In patients with small fragments which are unsuitable for reattachment, excision might be the best therapeutic option and lead to good outcomes. Whether using internal fixation or resection, the arthroscopic technique results in a better outcome and fewer complications, especially in older patients or athletes with overhead movement, because of the high incidence of shoulder impingement or rotator cuff tears which can be treated concurrently.

8.
Chin Med J (Engl) ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31490264

RESUMO

BACKGROUND: MicroRNAs (miRNAs) have played important roles in the regulation of gene expression in many cancers, but their roles in esophageal squamous cell carcinoma (ESCC) are still unclear. The aim of this study was to determine the potential ESCC-specific key miRNAs from a large sample dataset in The Cancer Genome Atlas (TCGA). METHODS: Integrative bioinformatics analysis was used to identify key ESCC-specific miRNAs related to the ESCC patients' tumor histological grade and lymphatic metastasis from TCGA. Next, these key miRNA potential gene regulatory functions and relationships with ESCC patients' clinical characteristics and overall survival were analyzed. Finally, three key miRNAs were selected randomly and quantificational real-time polymerase chain reaction (qRT-PCR) was used to validate in 51 newly diagnosed ESCC patients' tissues samples (collected from Nov. 2017 to Feb. 2019, in Wuwei, China) whether the bioinformatics analyses results were reliable and valid. Two-tailed Student's t test, Pearson chi-squared test and Kaplan-Meier survival analysis were used in this study. RESULTS: Thirty-five ESCC-specific miRNAs from TCGA database were investigated (fold-change > 2.0, P < 0.05), and 28 participated in the miRNAs-mRNAs co-expression network construction, while 17 were related with ESCC patients' tumor histological grade, TNM stage, and lymphatic metastasis (P < 0.05). Meanwhile, there were six miRNAs (including miR-200b-3p, miR-31-5p, miR-15b-5p, miR-141-3p, miR-135b-5p, and miR-195-5p) those were correlated with overall survival of ESCC patient (log-rank, P < 0.05). MiR-135b-5p, miR-15b-5p, and miR-195-5p were selected for verification of the expression levels in 51 ESCC patients' tissue samples by using qRT-PCR. We found that the fold-changes between qRT-PCR and TCGA were completely consistent. The results also suggested that miR-135b-5p, miR-15b-5p, and miR-195-5p were significantly correlated with tumor differentiation degrees (P < 0.05), miR-195-5p was significantly correlated with tumor TNM stage (P < 0.05), and miR-135b-5p was significantly correlated with lymph-node metastasis (P < 0.05). MiR-135b-5p, miR-15b-5p, and miR-195-5p expression levels, ESCC patient clinical features association analysis results and the aforementioned TCGA bioinformatics analyses were similar. CONCLUSION: This study identified key ESCC-related miRNAs. The key miRNAs are worthy of further investigation as potential novel biomarkers for diagnosis, classification, and prognosis of ESCC.

10.
J Ovarian Res ; 12(1): 79, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31470880

RESUMO

OBJECTIVE: To explore the risk factors for the recurrence of endometrioma and the risk factors for the recurrence of endometriosis-related pain after long-term follow-up. METHODS: This study retrospectively analyzed 358 women with endometriomas who had a minimum of 5-years follow up after laparoscopic endometrioma excision, which was performed at Peking Union Medical College Hospital from January 2009 to April 2013. All women were divided into recurrence group and nonrecurrence group. Analysis was performed with regard to preoperative history, laboratory analysis, findings during surgery, and symptoms during follow-up, including improvement and recurrence. RESULTS: The cumulative incidence rates of recurrence from 5 to 10 years after surgery were 15.4, 16.8, 19.3, 22.5, 22.5, and 22.5%, respectively. Significant differences were found between two groups in terms of age at surgery (RR: 0.764, 95% CI: 0.615-0.949, p = 0.015), duration of dysmenorrhea (RR: 1.120, 95% CI: 1.054-1.190, p < 0.001), presence of adenomyosis (RR: 1.629, 95% CI: 1.008-2.630, p = 0.046), CA125 level (RR: 1.856, 95% CI: 1.072-3.214, p = 0.021) and severity of dysmenorrhea. The severity of dysmenorrhea (RR: 1.711, 95% CI: 1.175-2.493, p = 0.005) and postoperative pregnancy (RR: 0.649, 95% CI: 0.460-0.914, p = 0.013) were significantly correlated with endometrioma recurrence in the multivariate analysis. No significant associations were found between the recurrence rate and gravida, parity, body mass index, infertility, leiomyoma presence, the size of ovarian endometrioma, the presence of deep infiltrating endometriosis, disease stage or postoperative medication. CONCLUSIONS: The severity of dysmenorrhea and postoperative pregnancy were independent risk factors for the recurrence of ovarian endometriomas after surgery during the long-time follow up.

11.
Anal Chim Acta ; 1082: 30-36, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31472710

RESUMO

The determination accuracy of alloying elements in high alloy steel is generally poor in laser-induced breakdown spectroscopy (LIBS) due to their matrix effect. To solve this problem, an image quantitative analysis (IQA) method was proposed and verified by determining nickel (Ni) in 17 stainless steel samples in this work. The results showed that the coefficient of determination (R2) was increased from 0.9833 of a conventional spectrum quantitative analysis (SQA) method to 0.9996 of the IQA method, and the average relative error of cross-validation (ARECV) and root mean squared error of cross-validation (RMSECV) were decreased from 56.80% and 1.0818 wt% to 15.93% and 0.9866 wt%, respectively. Besides, the determinations of chromium (Cr) and silicon (Si) demonstrated the generalization ability of the IQA. This study provides an effective approach to improving the quantitative performance of LIBS through the combination of image processing and computer vision technology.

12.
Environ Sci Technol ; 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31403792

RESUMO

Photochemical weathering leads to degradation of microplastics and releases chemical additives, polymeric fragments, and/or byproducts. This study evaluated the release kinetics of organotin compounds (OTCs) from three different sized (10-300 µm) polyvinyl chloride (PVC) microplastics under UV- and visible light irradiation. Four OTCs, dimethyltin (DMT), monomethyltin (MMT), dibutyltin (DBT), and monobutyltin (MBT), were found to release from PVC particles after 24 h leaching in darkness ranging from 2 to 20 µg·g-PVC-1. Under UV/visible light irradiation, only DMT and DBT were detectable, whereas MMT and MBT were not detected due to rapid photodegradation. The total tin concentrations (including organic and inorganic tins) in the aqueous phase monotonically increased under light exposure. By contrast, they reached plateaus after 24 h in darkness, confirming the photodegradation of OTCs. A release kinetics model was established and correctly interpreted the microplastics size effect on the OTC release process. Finally, the impacts of salinity and dissolved organic matter (DOM) were investigated. The release and photodegradation of OTCs were both inhibited at high salinity conditions, probably due to the enhanced readsorption of OTCs on PVC microplastics and the formation of halogen radicals that were less reactive toward neutral OTCs. The presence of DOM, however, increased OTCs release probably because the excited state triplet DOM (3DOM*) formed and reacted with OTCs from PVC microplastics.

13.
J Infect ; 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31442461

RESUMO

OBJECTIVES: Microbiological diagnosis is essential during clinical management of focal infections. Metagenomic next generation sequencing (mNGS) has been reported as a promising diagnostic tool in infectious diseases. However, little is known about the clinical utility of mNGS in focal infections. METHODS: We conducted a single-center retrospective study to investigate impact of mNGS on focal infection diagnosis and compared it with conventional methods, including culture, pathological examination, Xpert MTB/RIF, etc. 98 suspected focal infections cases were enrolled, and medical records were reviewed to determine their rates of detection, time-to-identification, and clinical outcomes. RESULTS: mNGS showed a satisfying diagnostic positive percent agreement of 86.30% (95% CI: 75.79%-92.88%) in a variety of tissues, compared to 45.21% (95% CI: 33.68%-57.24%) for culture and 57.53% (95% CI: 45.43%-68.84%)f for conventional methods (p<0.0125), and detected an extra 34 pathogenic microorganisms. Time requirement for pathogen identification using mNGS ranges from 31 hours to 55 hours, which showed an advantage over culture. (82.36 hours; 95%CI: 65.83,98.89; P<0.05) CONCLUSIONS: mNGS showed promising potential in pathogenic diagnosis during focal infections and might enable clinicians to make more timely and targeted therapeutic decisions.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31440476

RESUMO

The presence of carbapenem-producing Klebsiella pneumoniae (CP-Kp) is a serious threat to the control of nosocomial infections. Plasmid-mediated horizontal transfer of the resistance gene makes it difficult to control hospital-acquired CP- Kp infections. Nine CP- Kp strains were isolated during an outbreak in the intensive care unit of Shanghai Huashan hospital in east China. We conducted a retrospective study to identify the origin and route of transmission of this CP-Kp outbreak. Whole-genome sequencing (WGS) analysis was performed on 9 clinical isolates obtained from 8 patients, and the results were compared to clinical and epidemiological records. All isolates were ST11 CP-Kp. Single-nucleotide polymorphisms and the presence and structure of plasmids indicated that this CP-Kp outbreak had different origins. These 9 isolates were partitioned into two clades according to genetic distance. Four plasmids, CP002474.1, CP006799.1, CP018455.1, and CP025459.1, were detected among the 9 isolates. The plasmid phylogeny and antibiotic resistance (AR) gene profile results were consistent with the sequencing results. We found that two clades of CP-Kp were responsible for this nosocomial outbreak and demonstrated the transmission route from two index patients. Plasmid carriage and phylogeny are a useful tool for identifying clades involved in disease transmission.

15.
Medicine (Baltimore) ; 98(33): e16778, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31415381

RESUMO

BACKGROUND: Tenofovir disoproxil fumarate (TDF) has been widely recommended as a first-line antiviral agent to treat chronic hepatitis B (CHB). Qingzhong and Viread, formulations of TDF commercialized by Jiangsu Chia-tai Tianqing Pharmaceutical Co Ltd and GlaxoSmithKline, respectively, have both been approved by the State Food and Drug Administration, China. This study analyzed the efficacy and safety of these 2 TDF agents in Chinese patients with CHB. METHODS: In this multicenter, randomized, double-blind, double-dummy, noninferiority phase 3 clinical trial (ClinicalTrials.gov identifier: NCT02287857), 330 Chinese patients with CHB [hepatitis B envelope antigen-positive (HBeAg) (+): 232] were randomly assigned to receive Qingzhong (group A: 161 patients) or Viread (group B: 169 patients) 300 mg once daily for 48 weeks. Subsequently, all patients were administered Qingzhong 300 mg once daily from week 49 to week 240. The primary end point was the degree of decline of plasma hepatitis B virus (HBV) DNA levels at week 48 and the secondary endpoints were viral suppression, normalization of alanine aminotransferase (ALT) levels, hepatitis B surface antigen (HBsAg)/HBeAg loss or seroconversion, and virological breakthrough. RESULTS: Among patients with CHB who were HBeAg (+), the mean HBV DNA titer decreased similarly between the groups at week 48. The percentages of patients who achieved undetectable HBV DNA were similar between the groups (85.11% and 82.35% in groups A and B, respectively) and similar losses of HBeAg and HBeAg seroconversion rates were achieved. Moreover, for patients with CHB who were HBeAg (-), reductions in HBV DNA were similar. Among all patients with CHB, the rates of normalization of ALT and the loss of HBsAg were similar. The overall incidence of adverse events was comparable between the groups. CONCLUSION: In conclusion, the 48-week administration of Qingzhong showed noninferior efficacy and safety profiles compared to Viread in Chinese patients with CHB.


Assuntos
Antivirais/uso terapêutico , Hepatite B Crônica/tratamento farmacológico , Tenofovir/uso terapêutico , Adolescente , Adulto , Idoso , Antivirais/administração & dosagem , China , Método Duplo-Cego , Esquema de Medicação , Composição de Medicamentos , Feminino , Antígenos E da Hepatite B/sangue , Hepatite B Crônica/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Tenofovir/administração & dosagem , Resultado do Tratamento , Adulto Jovem
16.
Vaccine ; 37(38): 5681-5687, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31421931

RESUMO

BACKGROUND: News coverage supportive of human papillomavirus (HPV) vaccination is widespread on the Internet. Meanwhile, large numbers of online comments questioning HPV vaccine safety, attacking vaccine manufacturers or providing personal experience of side effects may reduce the persuasion effect. This study explored how and under what conditions such comments influence the behavioral intention of subsequent youth female viewers or readers. METHODS: We recruited a random sample of 239 Chinese undergraduate female students from different, non-medicine-related disciplines and conducted a 2 (valence of online comments: positive vs. negative) × 2 (presentation of comments: narrative vs. descriptive) between-subjects factorial design study. After carrying two ANOVAs to examine the effects of experimental conditions, we established a simple mediation and a moderated mediation analysis to test hypothesis. RESULTS: Perceived risk of HPV emerged as a significant mediator in the moderated mediation model. Particularly under the negative condition, the narrative (vs. descriptive) comments increased behavioral intention to receive the vaccine (B = -0.20, SE = 0.10, 95% CI = -0.40 to -0.01) while they increased participants' perceived risk of HPV (B = -0.14, SE = 0.05, 95% CI = -0.27 to -0.06). However, under the positive condition, there was no significant difference in either risk perception or behavioral intention between the effects of narrative and descriptive comments on participants. CONCLUSIONS: Online comments do influence participants' perceived risk of HPV and vaccination intention. Perceived risk of HPV fully mediates the effect of comments on vaccination intention. More importantly, the indirect effect of narrative (vs. descriptive) comments on behavioral intention through risk perception is moderated by the valence of comments, and significant only under the negative comments condition.

17.
J Clin Lab Anal ; : e23002, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31420917

RESUMO

BACKGROUND: At present, gastric cancer (GC) is a serious threat to human life and health. Non-coding circular RNAs (circRNAs) have been found abnormal expression in multiple tumors. However, circRNAs remain largely unknown in tumor progression. In the present study, we mainly examined the expression, function, and molecular mechanisms of a new circRNAs (hsa_circ_101882) in GC. MATERIALS AND METHODS: The expression of hsa_circ_101882 in GC tissue, corresponding adjacent normal tissues, and GC cell lines was examined by RT-PCR. The function of hsa_circ_101882 in GC was evaluated by MTT assay, cell migration, and invasion assay, colony formation assay, and flow cytometric assay. The effect of hsa_circ_101882 on epithelial-to-mesenchymal transition (EMT)-related gene expression was detected by RT-PCR and Western blot. RESULTS: Hsa_circ_101882 expression levels were significantly increased in GC tissue and GC cell lines. Functionally, low expression of hsa_circ_101882 revealed anti-tumor effects via inhibiting cell growth, migration, and invasion and promoting cell apoptosis. Mechanically, the dysregulated expression of hsa_circ_101882 affects EMT signaling pathway, which was examined by detecting E-cadherin, N-cadherin, vimentin, and Snail expression levels. CONCLUSIONS: Therefore, our research reveals that hsa_circ_101882 is considered a metastasis promoter by activating EMT and may serve as a critical oncogene and potential new biomarker in GC.

18.
Cell Mol Biol Lett ; 24: 52, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31428167

RESUMO

Background: Hepatic stellate cell (HSC) activation is a central event during hepatic fibrosis. Aerobic glycolysis is one of its metabolic hallmarks. Blocking glycolysis is a novel therapeutic option for liver fibrosis. This study investigated the effects of costunolide, a natural product demonstrated to have hepatoprotective effects, on HSC activation and glycolysis. Methods: Primary HSCs were isolated from rats and cultured through 5 to 6 passages. Cell viability, activation markers, and glycolytic metabolism were examined in primary HSCs using various cellular and molecular approaches. Results: At 30 µM, costunolide reduced the viability of HSCs and inhibited the expression of α-smooth muscle actin and collagen I, two key markers of HSC activation. It also decreased glucose uptake and consumption, and reduced the intracellular levels of lactate in HSCs. At 10 mM, the glycolysis inhibitor 2-DG had a similar impact to costunolide at 30 µM: it significantly downregulated the expression of HSC activation markers. The combination of the two compounds produced more remarkable effects. Furthermore, costunolide repressed the expression and activity of hexokinase 2 (HK2), a pivotal rate-limiting enzyme that regulates glycolysis. However, overexpression of HK2 via plasmid transfection significantly reversed the costunolide-mediated downregulation of activation markers in HSCs, indicating that suppression of HK2 was required for costunolide to inhibit glycolysis-associated HSC activation. Conclusions: Our results show that costunolide can suppress HSC activation, and this is associated with inhibition of HK2, which blocks aerobic glycolysis. This suggests that costunolide is an antifibrotic candidate with potential for further development.

19.
BMJ Open ; 9(8): e026445, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31434764

RESUMO

OBJECTIVES: The objective of this meta-analysis was to assess whether depression in percutaneous coronary intervention (PCI) patients is associated with higher risk of adverse outcomes. DESIGN: Systematic review and meta-analysis. METHODS: EMBASE, PubMed, CINAHL and PsycINFO were searched as data sources. We selected prospective cohort studies evaluating the relationship between depression and any adverse medical outcome, including all-cause mortality, cardiac mortality and non-fatal events, from inception to 28 February 2019. Two reviewers independently extracted information and calculated the risk of cardiovascular events in patients with preoperative or postoperative depression compared with non-depressed patients. RESULTS: Eight studies (n=3297) met our inclusion criteria. Most studies found a positive association between depression and adverse cardiovascular outcomes. Meta-analysis yielded an aggregate risk ratio of 1.57 (95% CI 1.28 to 1.92, p<0.0001) for the magnitude of the relation between depression and adverse outcomes. CONCLUSIONS: Our systematic review and meta-analysis suggests that depression is associated with an increased risk of worse clinical outcome or mortality in patients undergoing PCI. Assessment time and length of follow-up do not have a significant effect on this conclusion.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31425118

RESUMO

Powered prostheses are effective for helping amputees walk in a single environment, but these devices are inconvenient to use in complex environments. In order to help amputees walk in complex environments, prostheses need to understand the motion intent of amputees. Recently, researchers have found that vision sensors can be utilized to classify environments and predict the motion intent of amputees. Although previous studies have been able to classify environments accurately in offline analysis, the corresponding time delay has not been considered. To increase the accuracy and decrease the time delay of environmental classification, the present paper proposes a new decision fusion method. In this method, the sequential decisions of environmental classification are fused by constructing a hidden Markov model and designing a transition probability matrix. The developed method is evaluated by inviting five able-bodied subjects and three amputees to perform indoor and outdoor walking experiments. The results indicate that the proposed method can classify environments with accuracy improvements of 1.01% (indoor) and 2.48% (outdoor) over the previous voting method when a delay of only one frame is incorporated. The present method also achieves higher classification accuracy than with the methods of recurrent neural network (RNN), long-short term memory (LSTM), and gated recurrent unit (GRU). When achieving the same classification accuracy, the method of the present paper can decrease the time delay by 67 ms (indoor) and 733 ms (outdoor) in comparison to the previous voting method. Besides classifying environments, the proposed decision fusion method may be able to optimize the sequential predictions of the human motion intent.

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