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1.
EMBO J ; : e101515, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31617603

RESUMO

The phytohormone auxin controls plant growth and development via TIR1-dependent protein degradation of canonical AUX/IAA proteins, which normally repress the activity of auxin response transcription factors (ARFs). IAA33 is a non-canonical AUX/IAA protein lacking a TIR1-binding domain, and its role in auxin signaling and plant development is not well understood. Here, we show that IAA33 maintains root distal stem cell identity and negatively regulates auxin signaling by interacting with ARF10 and ARF16. IAA33 competes with the canonical AUX/IAA repressor IAA5 for binding to ARF10/16 to protect them from IAA5-mediated inhibition. In contrast to auxin-dependent degradation of canonical AUX/IAA proteins, auxin stabilizes IAA33 protein via MITOGEN-ACTIVATED PROTEIN KINASE 14 (MPK14) and does not affect IAA33 gene expression. Taken together, this study provides insight into the molecular functions of non-canonical AUX/IAA proteins in auxin signaling transduction.

2.
J Exp Bot ; 70(21): 6163-6179, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31598687

RESUMO

Pentatricopeptide repeat (PPR) proteins are one of the largest protein families, which consists of >400 members in most species. However, the molecular functions of many PPR proteins are still uncharacterized. Here, we isolated a maize mutant, defective kernel 40 (dek40). Positional cloning, and genetic and molecular analyses revealed that DEK40 encodes a new E+ subgroup PPR protein that is localized in the mitochondrion. DEK40 recognizes and directly binds to cox3, nad2, and nad5 transcripts and functions in their processing. In the dek40 mutant, abolishment of the C-to-U editing of cox3-314, nad2-26, and nad5-1916 leads to accumulated reactive oxygen species and promoted programmed cell death in endosperm cells due to the dysfunction of mitochondrial complexes I and IV. Furthermore, RNA sequencing analysis showed that gene expression in some pathways, such as glutathione metabolism and starch biosynthesis, was altered in the dek40 mutant compared with the wild-type control, which might be involved in abnormal development of the maize mutant kernels. Thus, our results provide solid evidence on the molecular mechanism underlying RNA editing by DEK40, and extend our understanding of PPR-E+ type protein in editing functions and kernel development in maize.

3.
Plant Biotechnol J ; 2019 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-31350929

RESUMO

Tillering is a significant agronomic trait in wheat which shapes plant architecture and yield. Strigolactones (SLs) function in inhibiting axillary bud outgrowth. The roles of SLs in the regulation of bud outgrowth have been described in model plant species, including rice and Arabidopsis. However, the role of SLs genes in wheat remains elusive due to the size and complexity of the wheat genomes. In this study, TaD27 genes in wheat, orthologs of rice D27 encoding an enzyme involved in SLs biosynthesis, were identified. TaD27-RNAi wheat plants had more tillers, and TaD27-B-OE wheat plants had fewer tillers. Germination bioassay of Orobanche confirmed the SLs was deficient in TaD27-RNAi and excessive in TaD27-B-OE wheat plants. Moreover, application of exogenous GR24 or TIS108 could mediate the axillary bud outgrowth of TaD27-RNAi and TaD27-B-OE in the hydroponic culture, suggesting that TaD27-B plays critical roles in regulating wheat tiller number by participating in SLs biosynthesis. Unlike rice D27, plant height was not affected in the transgenic wheat plants. Transcription and gene coexpression network analysis showed that a number of genes are involved in the SLs signalling pathway and axillary bud development. Our results indicate that TaD27-B is a key factor in the regulation of tiller number in wheat.

4.
Trends Plant Sci ; 24(9): 802-809, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31257155

RESUMO

The inflorescence architecture of grass crops affects the number of kernels and final grain yield. Great progress has been made in genetic analysis of rice inflorescence development in the past decades. However, the advances in wheat largely lag behind those in rice due to the repetitive and polyploid genomes of wheat. In view of the similar branching patterns and developmental characteristics between rice and wheat, the studies on inflorescence architecture in rice will facilitate related studies in wheat in the future. Here, we review the developmental regulation of inflorescences in rice and wheat and highlight several pathways that potentially regulate the inflorescence architecture of wheat.

5.
Plant Biotechnol J ; 2019 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-31199059

RESUMO

Heterosis, or hybrid vigour, is a predominant phenomenon in plant genetics, serving as the basis of crop hybrid breeding, but the causative loci and genes underlying heterosis remain unclear in many crops. Here, we present a large-scale genetic analysis using 5360 offsprings from three elite maize hybrids, which identifies 628 loci underlying 19 yield-related traits with relatively high mapping resolutions. Heterotic pattern investigations of the 628 loci show that numerous loci, mostly with complete-incomplete dominance (the major one) or overdominance effects (the secondary one) for heterozygous genotypes and nearly equal proportion of advantageous alleles from both parental lines, are the major causes of strong heterosis in these hybrids. Follow-up studies for 17 heterotic loci in an independent experiment using 2225 F2 individuals suggest most heterotic effects are roughly stable between environments with a small variation. Candidate gene analysis for one major heterotic locus (ub3) in maize implies that there may exist some common genes contributing to crop heterosis. These results provide a community resource for genetics studies in maize and new implications for heterosis in plants.

6.
J Integr Plant Biol ; 2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31119902

RESUMO

Mitochondria, the main energy transducers in plant cells, require the proper assembly of respiratory chain complexes I-V for their function. The NADH dehydrogenase 4 (nad4) gene encodes mitochondrial respiratory chain complex I subunit IV, but the mechanism underlying nad4 transcript splicing is unclear. Here, we report that the P-type pentatricopeptide repeat (PPR) protein DEFECTIVE KERNEL 43 (DEK43) is responsible for cis-splicing of the nad4 transcript in maize. We demonstrate that DEK43 localizes to both the nucleus and mitochondria. The mutation of Dek43 resulted in embryo-lethal and light-colored defective kernels. Among the 22 mitochondrial group II introns, the splicing efficiency of nad4 introns 1 and 3 was reduced by up to 50% compared to the wild type. The levels of complex I and supercomplex I+III2 were also reduced in dek43. Furthermore, in-gel NADH dehydrogenase assays indicated that the activities of these complexes were significantly reduced in dek43. Further, the mitochondrial ultrastructure was altered in the mutant. Together, our findings indicate that DEK43, a dual-localized PPR protein, plays an important role in maintaining mitochondrial function and maize kernel development.

7.
Zhonghua Nan Ke Xue ; 24(6): 558-561, 2018 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-30173464

RESUMO

Nanos2, a member of the Nanos2 gene family, is a specific gene in male germ cells and encodes an evolutionarily conserved RNA binding protein expressed in male primordial germ cells (PGCs) during the embryonic period as well as in the spermatogonial stem cells (SSCs) of the testis. In the embryonic period, Nanos2 promotes the development of male PGCs and inhibits them from meiosis. In the process of spermatogenesis, Nanos2 suppresses the differentiation of SSCs in the testis and maintains the stability of the SSC pool. The knockout of Nanos2 may cause the disappearance of germ cells and sterility in male mice while its overexpression in the testis may lead to accumulation of SSCs in seminiferous tubules. Besides, Nanos2 is involved in the degradation of specific RNAs and possibly associated with some diseases of the male reproductive system. This review focuses on the recent progress in the studies of Nanos2 in the male reproductive system.


Assuntos
Diferenciação Celular , Proteínas de Ligação a RNA/genética , Espermatozoides , Animais , Técnicas de Inativação de Genes , Masculino , Meiose , Camundongos , RNA/metabolismo , Proteínas de Ligação a RNA/metabolismo , Espermatogênese/fisiologia , Espermatogônias , Testículo/citologia
8.
Zhonghua Nan Ke Xue ; 24(3): 268-271, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-30161315

RESUMO

Premature ejaculation (PE), as one of the most common male sexual dysfunctions, has a serious negative impact on the sexual satisfaction of the patients and their sexual partners. Lifelong PE is a most common type and a current focus of research as well. The etiology and pathogenesis of this disease are not yet clear and genetic factors are considered to be closely related to lifelong PE. Studies show that the 5-hydroxytryptamine transporter (5-HTT) gene plays an important role in the development and progression of lifelong premature ejaculation and the 5-HTT-linked polymorphic region (5-HTTLPR) has attracted much attention in recent years. This article presents an overview on the correlation between 5-HTTLPR and lifelong PE.


Assuntos
Polimorfismo Genético , Ejaculação Precoce/genética , Proteínas da Membrana Plasmática de Transporte de Serotonina/genética , Adulto , Ejaculação , Humanos , Masculino
9.
Basic Clin Pharmacol Toxicol ; 123(6): 714-720, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29972887

RESUMO

Oxidative stress and inflammatory responses are closely implicated in the progression of renal interstitial fibrosis, thereby leading to chronic kidney disease. Cryptotanshinone (CTS) is a natural compound involved in antioxidant and anti-inflammatory activities. We evaluated the effects of CTS on inflammation and oxidative stress in obstructed kidneys. Mice received gastric gavage of CTS from 7 days before unilateral ureteral obstruction operation to 1 week after surgery. Administration of CTS at 50 and 100 mg/kg/day significantly decreased collagen production, as shown by Masson staining. Immunohistochemistry staining and RT-PCR confirmed that CTS reduced extracellular matrix proteins, such as fibronectin and collagen-1, in the obstructed kidneys in a dose-dependent manner. Furthermore, immunohistochemistry staining indicated that CTS inhibited infiltration of the macrophage (CD68-positive) and lymphocyte (CD3-positive) cells, which were associated with the suppression of the nuclear factor-κB signalling activation. CTS increased superoxide dismutase, catalase and glutathione while decreased malondialdehyde production. More importantly, CTS activated Nrf-2 and HO-1 in the obstructed kidneys for 7 days. CTS could protect renal interstitial fibrosis by ameliorating inflammation and oxidative stress, which might be through the regulation of NF-κB and Nrf-2/HO-1 signalling pathways.


Assuntos
Anti-Inflamatórios/uso terapêutico , Antioxidantes/uso terapêutico , Inflamação/tratamento farmacológico , Fator 2 Relacionado a NF-E2/efeitos dos fármacos , NF-kappa B/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Fenantrenos/uso terapêutico , Obstrução Ureteral/tratamento farmacológico , Animais , Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Western Blotting , Rim/patologia , Camundongos , Camundongos Endogâmicos C57BL , Fenantrenos/farmacologia , Reação em Cadeia da Polimerase em Tempo Real , Obstrução Ureteral/complicações , Obstrução Ureteral/patologia
10.
Trends Plant Sci ; 23(8): 660-666, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29880405

RESUMO

Pluripotent stem cells (PSCs) are self-renewable cells with the potential to differentiate into all the cell types within an organism. PSCs exist transiently in early-stage mammalian embryos during ontogeny and are maintained in apical meristems of higher plants throughout postembryonic development. Through proper in vitro culture, somatic cells of both mammals and plants can be reprogrammed to generate induced PSCs (iPSCs). Recent studies have deciphered mechanisms underlying pluripotency gene activation and cell fate transition during plant iPSC generation. Here, we compare these mechanisms with those of their animal counterparts in the hope that this may trigger mutual learning of researchers from both fields, leading to advances and independent breakthroughs in this important area.


Assuntos
Fenômenos Fisiológicos Vegetais , Plantas/genética , Células-Tronco Pluripotentes/fisiologia , Animais , Diferenciação Celular , Células-Tronco Pluripotentes Induzidas/fisiologia , Meristema/genética , Meristema/fisiologia
11.
Plant J ; 95(1): 150-167, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29752751

RESUMO

Plant meristem activity depends on accurate execution of transcriptional networks required for establishing optimum functioning of stem cell niches. An Arabidopsis mutant card1-1 (constitutive auxin response with DR5:GFP) that encodes a truncated RPB1 (RNA Polymerase II's largest subunit) with shortened C-terminal domain (CTD) was identified. Phosphorylation of the CTD repeats of RPB1 is coupled to transcription in eukaryotes. Here we uncover that the truncated CTD of RPB1 disturbed cell cycling and enlarged the size of shoot and root meristem. The defects in patterning of root stem cell niche in card1-1 indicates that intact CTD of RPB1 is necessary for fine-tuning the specific expression of genes responsible for cell-fate determination. The gene-edited plants with different CTD length of RPB1, created by CRISPR-CAS9 technology, confirmed that both the full length and the DK-rich tail of RPB1's CTD play roles in the accurate transcription of CYCB1;1 encoding a cell-cycle marker protein in root meristem and hence participate in maintaining root meristem size. Our experiment proves that the intact RPB1 CTD is necessary for stem cell niche maintenance, which is mediated by transcriptional regulation of cell cycling genes.


Assuntos
Proteínas de Arabidopsis/fisiologia , Arabidopsis/fisiologia , Ciclo Celular/fisiologia , RNA Polimerases Dirigidas por DNA/fisiologia , Nicho de Células-Tronco/fisiologia , Proteína 9 Associada à CRISPR , Sistemas CRISPR-Cas , Edição de Genes , Regulação da Expressão Gênica de Plantas , Meristema/metabolismo , Raízes de Plantas/metabolismo , Brotos de Planta/metabolismo , Plantas Geneticamente Modificadas
12.
Plant Physiol ; 177(2): 819-832, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29720555

RESUMO

Auxin has been shown to enhance root growth inhibition under aluminum (Al) stress in Arabidopsis (Arabidopsis thaliana). However, in maize (Zea mays), auxin may play a negative role in the Al-induced inhibition of root growth. In this study, we identified mutants deficient in the maize auxin efflux carrier P-glycoprotein (ZmPGP1) after ethyl methanesulfonate mutagenesis and used them to elucidate the contribution of ZmPGP1 to Al-induced root growth inhibition. Root growth in the zmpgp1 mutant, which forms shortened roots and is hyposensitive to auxin, was less inhibited by Al stress than that in the inbred line B73. In the zmpgp1 mutants, the root tips displayed higher auxin accumulation and enhanced auxin signaling under Al stress, which was also consistent with the increased expression of auxin-responsive genes. Based on the behavior of the auxin-responsive marker transgene, DR5rev:RFP, we concluded that Al stress reduced the level of auxin in the root tip, which contrasts with the tendency of Al stress-induced Arabidopsis plants to accumulate more auxin in their root tips. In addition, Al stress induced the expression of ZmPGP1 Therefore, in maize, Al stress is associated with reduced auxin accumulation in root tips, a process that is regulated by ZmPGP1 and thus causes inhibition of root growth. This study provides further evidence about the role of auxin and auxin polar transport in Al-induced root growth regulation in maize.


Assuntos
Alumínio/toxicidade , Ácidos Indolacéticos/metabolismo , Proteínas de Plantas/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Zea mays/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Meristema/efeitos dos fármacos , Meristema/genética , Meristema/metabolismo , Mutação , Ácidos Naftalenoacéticos/farmacologia , Proteínas de Plantas/genética , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Plantas Geneticamente Modificadas , Transdução de Sinais , Zea mays/genética , Zea mays/crescimento & desenvolvimento , Zea mays/metabolismo
13.
J Agric Food Chem ; 66(13): 3465-3476, 2018 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-29522338

RESUMO

To study the mechanism of anthocyanin-biosynthesis regulation, we examined light-regulated gene expression involved in anthocyanin biosynthesis in purple grains of wheat. Ten kinds of anthocyanins were identified from a purple-grained wheat cultivar by HPLC-ESI-MS/MS analysis. Libraries constructed from the total RNA of purple grains under light (L) or dark (D) conditions for 15 or 20 days were sequenced. In total, 1874 differentially expressed genes (DEGs) were identified in L20 vs L15, 1432 DEGs were identified in D20 vs D15, 862 DEGs were identified in D15 vs L15, and 1786 DEGs were identified in D20 vs L20. DEG functional enrichments suggested that light-signal transduction is critical to anthocyanin biosynthesis. The 911 DEGs referred to as light-regulated DEGs (LDEGs) involved a number of genes in anthocyanin biosynthesis, transcription regulation, sugar- and calcium-signaling pathways, and hormone metabolism. These findings laid the foundation for future studies on the regulatory mechanisms of anthocyanin biosynthesis in purple grains of wheat.


Assuntos
Antocianinas/biossíntese , Proteínas de Plantas/genética , Triticum/genética , Regulação da Expressão Gênica de Plantas/efeitos da radiação , Luz , Proteínas de Plantas/metabolismo , Sementes/genética , Sementes/metabolismo , Sementes/efeitos da radiação , Transcriptoma/efeitos da radiação , Triticum/metabolismo , Triticum/efeitos da radiação
14.
New Phytol ; 218(4): 1334-1339, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29574802

RESUMO

Contents Summary 1334 I. Introduction 1334 II. Regeneration-initial cell: the origin of regeneration 1335 III. Acquiring regeneration competency: the essential intermediate step for hormone-induced regeneration 1335 IV. Hormonal induction of stem cell regulators: the program for de novo establishment of apical meristems 1337 V. Conclusions and perspectives 1337 Acknowledgements 1338 Author contributions 1338 References 1338 SUMMARY: High cellular plasticity confers remarkable regeneration capacity to plants. Based on the activity of stem cells and their regulators, higher plants are capable of regenerating new individuals. De novo organogenesis exemplifies the regeneration of the whole plant body and is exploited widely in agriculture and biotechnology. In this Tansley insight article, we summarize recent advances that facilitate our understanding of the molecular mechanisms underlying de novo organogenesis. According to our current knowledge, this process can be divided into three steps, including activation of regeneration-initial cells, acquisition of competency and de novo establishment of apical meristems. The functions of stem cells and their regulators are critical to de novo organogenesis, whereas auxin and cytokinin act as triggers and linkers between different steps.


Assuntos
Organogênese , Células Vegetais/metabolismo , Células-Tronco/citologia , Meristema/efeitos dos fármacos , Meristema/crescimento & desenvolvimento , Organogênese/efeitos dos fármacos , Células Vegetais/efeitos dos fármacos , Reguladores de Crescimento de Planta/farmacologia , Regeneração/efeitos dos fármacos , Células-Tronco/efeitos dos fármacos
15.
Plant Cell Physiol ; 59(4): 756-764, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29186581

RESUMO

Plants are known for their capacity to regenerate organs, such as shoot, root and floral organs. Recently, a number of studies contributed to understanding the mechanisms of shoot and root regeneration. However, the mechanisms underlying floral organ regeneration are largely unknown. In this study, we established a carpel regeneration system in which two types of carpels were induced by exogenous cytokinin. For type I, all the floral organs in the regenerated inflorescence were transformed into carpels. For type II, carpels were generated directly from callus. The transcript level of AGAMOUS (AG), the carpel identity gene, was up-regulated during carpel induction. The expression signals of AG were detected in the initiating carpel primordia and regenerating carpels, and co-localized with those of two Type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), ARR1 and ARR10. Repression of either AG or type-B ARRs reduced carpel regeneration. Binding analyses showed that ARR1 and ARR10 directly bound to transcriptional regulatory regions of AG and positively regulated its expression. In addition, the expression of type-B ARRs overlapped with that of AG in the floral primordia in planta. Defects in type-B ARRs reduced the number of carpels. The results indicate that type-B ARRs control carpel regeneration through activating AG expression. Our results provide new information for understanding the mechanism of carpel formation.


Assuntos
Proteína AGAMOUS de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Proteínas de Ligação a DNA/metabolismo , Flores/fisiologia , Regulação da Expressão Gênica de Plantas , Regeneração , Fatores de Transcrição/metabolismo , Proteína AGAMOUS de Arabidopsis/metabolismo , Reprogramação Celular/efeitos dos fármacos , Citocininas/farmacologia , Flores/genética , Regeneração/efeitos dos fármacos
16.
Asian J Androl ; 20(3): 300-305, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29226878

RESUMO

This study aims to validate our hypothesis that acid-sensing ion channels (ASICs) may contribute to the symptom of pain in patients with chronic prostatitis (CP). We first established a CP rat model, then isolated the L5-S2 spinal dorsal horn neurons for further studies. ASIC1a was knocked down and its effects on the expression of neurogenic inflammation-related factors in the dorsal horn neurons of rat spinal cord were evaluated. The effect of ASIC1a on the Ca2+ ion concentration in the dorsal horn neurons of rat spinal cord was measured by the intracellular calcium ([Ca2+]i) intensity. The effect of ASIC1a on the p38/mitogen-activated protein kinase (MAPK) signaling pathway was also determined. ASIC1a was significantly upregulated in the CP rat model as compared with control rats. Acid-induced ASIC1a expression increased [Ca2+]i intensity in the dorsal horn neurons of rat spinal cord. ASIC1a also increased the levels of neurogenic inflammation-related factors and p-p38 expression in the acid-treated dorsal horn neurons. Notably, ASIC1a knockdown significantly decreased the expression of pro-inflammatory cytokines. Furthermore, the levels of p-p38 and pro-inflammatory cytokines in acid-treated dorsal horn neurons were significantly decreased in the presence of PcTx-1, BAPTA-AM, or SB203580. Our results showed that ASIC1a may contribute to the symptom of pain in patients with CP, at least partially, by regulating the p38/MAPK signaling pathway.


Assuntos
Canais Iônicos Sensíveis a Ácido/genética , Cálcio/metabolismo , Sistema de Sinalização das MAP Quinases/genética , Dor/genética , Células do Corno Posterior/metabolismo , Prostatite/complicações , Bloqueadores do Canal Iônico Sensível a Ácido/farmacologia , Animais , Quelantes/farmacologia , Doença Crônica , Citocinas/efeitos dos fármacos , Citocinas/metabolismo , Modelos Animais de Doenças , Ácido Egtázico/análogos & derivados , Ácido Egtázico/farmacologia , Técnicas de Silenciamento de Genes , Imidazóis/farmacologia , Inflamação/genética , Inflamação/metabolismo , Masculino , Dor/etiologia , Peptídeos/farmacologia , Fosforilação/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Piridinas/farmacologia , Ratos , Venenos de Aranha/farmacologia , Regulação para Cima , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
17.
Plant Physiol ; 176(3): 2231-2250, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-28724620

RESUMO

Plant regeneration is fundamental to basic research and agricultural applications. The regeneration capacity of plants varies largely in different genotypes, but the reason for this variation remains elusive. Here, we identified a novel thioredoxin DCC1 in determining the capacity of shoot regeneration among Arabidopsis (Arabidopsis thaliana) natural variation. Loss of function of DCC1 resulted in inhibited shoot regeneration. DCC1 was expressed mainly in the inner tissues of the callus and encoded a functional thioredoxin that was localized in the mitochondria. DCC1 protein interacted directly with CARBONIC ANHYDRASE2 (CA2), which is an essential subunit of the respiratory chain NADH dehydrogenase complex (Complex I). DCC1 regulated Complex I activity via redox modification of CA2 protein. Mutation of DCC1 or CA2 led to reduced Complex I activity and triggered mitochondrial reactive oxygen species (ROS) production. The increased ROS level regulated shoot regeneration by repressing expression of the genes involved in multiple pathways. Furthermore, linkage disequilibrium analysis indicated that DCC1 was a major determinant of the natural variation in shoot regeneration among Arabidopsis ecotypes. Thus, our study uncovers a novel regulatory mechanism by which thioredoxin-dependent redox modification regulates de novo shoot initiation via the modulation of ROS homeostasis and provides new insights into improving the capacity of plant regeneration.


Assuntos
Arabidopsis/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Tiorredoxinas/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Anidrases Carbônicas/genética , Anidrases Carbônicas/metabolismo , Complexo I de Transporte de Elétrons/genética , Complexo I de Transporte de Elétrons/metabolismo , Homeostase , Mitocôndrias/genética , Mitocôndrias/metabolismo , Mutação , Brotos de Planta/fisiologia , Plantas Geneticamente Modificadas , Polimorfismo de Nucleotídeo Único , Regeneração/fisiologia , Tiorredoxinas/genética
18.
Asian J Androl ; 20(1): 19-23, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-28361812

RESUMO

The premature ejaculation diagnostic tool (PEDT) is a brief diagnostic measure to assess premature ejaculation (PE). However, there is insufficient evidence regarding its validity in the new evidence-based-defined PE. This study was performed to evaluate the validity of PEDT and its association with IIEF-15 in different types of evidence-based-defined PE. From June 2015 to January 2016, a total of 260 men complaining of PE and defined as lifelong PE (LPE)/acquired PE (APE) according to the evidence-based definition from Andrology Clinic of the First Affiliated Hospital of Anhui Medical University, along with 104 male healthy controls without PE from a medical examination center, were enrolled in this study. All individuals completed questionnaires including demographics, medical and sexual history, as well as PEDT and IIEF-15. After statistical analysis, it was found that men with PE reported higher PEDT scores (14.28 ± 3.05) and lower IIEF-15 (41.26 ± 8.20) than men without PE (PEDT: 5.32 ± 3.42, IIEF-15: 52.66 ± 6.86, P < 0.001 for both). It was suggested that a score of ≥9 indicated PE in both LPE and APE by sensitivity and specificity analyses (sensitivity: 0.875, 0.913; specificity: 0.865, 0.865, respectively). In addition, IIEF-15 were higher in men with LPE (42.64 ± 8.11) than APE (39.43 ± 7.84, P < 0.001). After adjusting for age, IIEF-15 was negatively related to PEDT in men with LPE (adjust r = -0.225, P < 0.001) and APE (adjust r = -0.378, P < 0.001). In this study, we concluded that PEDT was valid in the diagnosis of evidenced-based-defined PE. Furthermore, IIEF-15 was negatively related to PEDT in men with different types of PE.


Assuntos
Disfunção Erétil/diagnóstico , Ejaculação Precoce/diagnóstico , Adulto , Envelhecimento , Grupo com Ancestrais do Continente Asiático , Medicina Baseada em Evidências , Feminino , Humanos , Masculino , Valores de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Fatores Socioeconômicos , Inquéritos e Questionários , Adulto Jovem
20.
New Phytol ; 217(1): 219-232, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28960381

RESUMO

DNA methylation plays a critical role in diverse biological processes of plants. Arabidopsis DNA METHYLTRANSFERASE1 (MET1) represses shoot regeneration by inhibiting WUSCHEL (WUS) expression, which is essential for shoot initiation. However, the upstream signals regulating MET1 expression during this process are unclear. We analyzed the signals regulating MET1 expression using a number of established strategies, such as genetic analysis, confocal microscopy, quantitative real-time PCR and chromatin immunoprecipitation. MET1 expression patterns underwent dynamic changes with the initiation of WUS during shoot regeneration. The cell cycle regulator E2FA was characterized as an upstream factor directly promoting MET1 expression. Moreover, cytokinin promoted MET1 expression partially by enhancing CYCD3 expression. Our findings reveal that MET1-mediated shoot regeneration is regulated by the cytokinin-induced cell cycle, and provide new insights into the regulation of DNA methylation in shoot regeneration.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimologia , Citocininas/metabolismo , DNA (Citosina-5-)-Metiltransferases/metabolismo , Reguladores de Crescimento de Planta/metabolismo , Arabidopsis/genética , Arabidopsis/fisiologia , Proteínas de Arabidopsis/genética , Ciclo Celular , Ciclinas/genética , Ciclinas/metabolismo , DNA (Citosina-5-)-Metiltransferases/genética , Metilação de DNA , Fatores de Transcrição E2F/genética , Fatores de Transcrição E2F/metabolismo , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Brotos de Planta/enzimologia , Brotos de Planta/genética , Brotos de Planta/fisiologia , Regeneração
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