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1.
Yi Chuan ; 41(8): 736-745, 2019 Aug 20.
Artigo em Chinês | MEDLINE | ID: mdl-31447424

RESUMO

As one of plant cell wall components, pectin is the main anti-nutritional factor in livestock and poultry feeds and has an adverse effect on utilization efficiency of feed energy and nitrogen. Pectinases, which are widely found in microorganisms such as bacteria, yeast and filamentous fungi in nature,can improve feed efficiency by relieving the anti-nutritional effect of pectin through promoting the hydrolysis reaction of feed pectin. To explore the feasibility of expressing microbial-derived pectinase genes in pig cells, we introduced microbial-derived pectinase genes pg5a, pgI, pga3A, and pgaA into porcine PK 15 cells by lipofection for heterogenous expression. Enzymatic activities of the pectinases encoded by these genes were analyzed using the 3,5 dinitrosalicylic acid (DNS) method. Results showed that all four pectinase genes were able to be transcribed into mRNAs in porcine PK 15 cells, but only pg5a and pgI were adapted to the porcine cell expression system. Among them, the maximum activity of pectinase PG5A was 0.95 U/mL, the optimum pH was pH 4.0, and the enzymatic activity was maintained above 46% in the range of pH 4.6 to 6.0. Pectinase PGI obtained the highest enzymatic activity at pH 5.0, which was 0.30 U/mL, and maintained more than 35% of the activity in the range of pH 4.0 to 6.0. The results of digestive protease tolerance test showed that PG5A and PGI were highly resistant to pepsin and trypsin. After treatment with 1 mg/mL pig pepsin for two hours, the residual enzymatic activities of PG5A and PGI were 76% and 71%, respectively. And after two hours treatment with 1 mg/mL of pig trypsin, the remaining enzymatic activities of PG5A and PGI were 44% and 93%, respectively. In summary, pectinase PG5A and PGI can be effectively expressed in pig cells, and have strong tolerance to pig intestinal pH environment and digestive proteases. Therefore, both pg5a and pgI can be used as candidate genes for production of transgenic pigs.


Assuntos
Bactérias/enzimologia , Fungos/enzimologia , Poligalacturonase/biossíntese , Animais , Células Cultivadas , Pectinas , Poligalacturonase/genética , Suínos
2.
Int J Mol Med ; 44(3): 835-846, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31257468

RESUMO

In this study, we focused on several itch­related molecules and receptors in the spinal cord with the goal of clarifying the specific mediators that regulate itch sensation. We investigated the involvement of serotonin receptors, opioid receptors, glia cell markers and chemokines (ligands and receptors) in models of acetone/ether/water (AEW)­ and diphenylcyclopropenone (DCP)­induced chronic itch. Using reverse transcription­quantitative polymerase chain reaction, we examined the expression profiles of these mediators in the lower cervical spinal cord (C5­8) of two models of chronic itch. We found that the gene expression levels of opioid receptor mu 1 (Oprm1), 5­hydroxytryptamine receptor 1A (Htr1a) and 5­hydroxytryptamine receptor 6 (Htr6) were upregulated. Among the chemokines, the expression levels of C­C motif chemokine ligand (Ccl)21, Cxcl3 and Cxcl16 and their receptors, Ccr7, Cxcr2 and Cxcr6, were simultaneously upregulated in the spinal cords of the mice in both models of chronic itch. By contrast, the expression levels of Ccl2, Ccl3, Ccl4 and Ccl22 were downregulated. These findings indicate that multiple mediators, such as chemokines in the spinal cord, are altered and may be central candidates in further research into the mechanisms involved in the development of chronic itch.


Assuntos
Biomarcadores , Medula Cervical/metabolismo , Regulação da Expressão Gênica , Prurido/genética , Animais , Biópsia , Quimiocinas/metabolismo , Doença Crônica , Modelos Animais de Doenças , Perfilação da Expressão Gênica , Imuno-Histoquímica , Masculino , Camundongos , Microglia/metabolismo , Neurônios/metabolismo , Prurido/diagnóstico , Prurido/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores Opioides mu/genética , Receptores Opioides mu/metabolismo , Pele/metabolismo , Pele/patologia
3.
Yi Chuan ; 41(4): 327-336, 2019 Apr 20.
Artigo em Chinês | MEDLINE | ID: mdl-30992254

RESUMO

There are two major pathways, homology-directed repair (HDR) and nonhomologous end joining (NHEJ), involved in double-strand break (DSB) repair. Single-stranded oligodeoxyribonucleotide (ssODN)-mediated homologous recombination repair is commonly used for animal site-directed genome editing, with great scientific and practical value. To improve ssODN-mediated HDR efficiency in the pig genome, we investigated the effect and molecular mechanism of mitogen-activated extracellular signal-regulated kinase (MEK) inhibitor PD0325901 on the HDR efficiency in porcine fetal fibroblasts (PFFs). The results showed that PD0325901 obviously increased the percentage of G2 and S phase cell populations and reduced the cell population ratio in the G1 phase of PFFs, and promoted the expression of HDR repair factor. At the optimal concentration of 250 nmol/L, PD0325901 increased the repair efficiency of ssODN-mediated GFP reporter vector by 58.8% and the directed editing efficiency of PFF DMD and ROSA26 locus by 48.16% and 17.64%, respectively. The results show that MEK inhibitor PD0325901 significantly promotes the efficiency of ssODN-mediated homologous-directed repair in the porcine genome, thus offering a new idea to generate genetically modified pigs more effectively.


Assuntos
Benzamidas/farmacologia , Difenilamina/análogos & derivados , Edição de Genes , Reparo de DNA por Recombinação , Animais , Quebras de DNA de Cadeia Dupla , DNA de Cadeia Simples , Difenilamina/farmacologia , Fibroblastos , MAP Quinase Quinase Quinase 1/antagonistas & inibidores , Oligodesoxirribonucleotídeos , Suínos
4.
Yonsei Med J ; 60(2): 163-173, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30666838

RESUMO

PURPOSE: This study was undertaken to explore how miR-206 represses osteosarcoma (OS) development. MATERIALS AND METHODS: Expression levels of miR-206, PAX3, and MET mRNA were explored in paired OS and adjacent tissue specimens. A patient-derived OS cell line was established. miR-206 overexpression and knockdown were achieved by lentiviral transduction. PAX3 and MET overexpression were achieved by plasmid transfection. Treatment with hepatocyte growth factor (HGF) was utilized to activate c-Met receptor. Associations between miR-206 and PAX3 or MET mRNA in OS cells were verified by AGO2-RNA immunoprecipitation assay and miRNA pulldown assay. OS cell malignancy was evaluated in vitro by cell proliferation, metastasis, and apoptosis assays. PAX3 and MET gene expression in OS cells was assayed by RT-qPCR and Western blot. Activation of PI3K-AKT and MAPK-ERK in OS cells were assayed by evaluating Akt1 Ser473 phosphorylation and total threonine phosphorylation of Erk1/2, respectively. RESULTS: Expression levels of miR-206 were significantly decreased in OS tissue specimens, compared to adjacent counterparts, and were inversely correlated with expression of PAX3 and MET mRNA. miR-206 directly interacted with PAX3 and MET mRNA in OS cells. miR-206 overexpression significantly reduced PAX3 and MET gene expression in OS cells in vitro, resulting in significant decreases in Akt1 and Erk1/2 activation, cell proliferation, and metastasis, as well as increases in cell apoptosis, while miR-206 knockdown showed the opposite effects. The effects of miR-206 overexpression on OS cells were reversed by PAX3 or MET overexpression, but only partially attenuated by HGF treatment. CONCLUSION: miR-206 reduces OS cell malignancy in vitro by targeting PAX3 and MET gene expression.


Assuntos
Epitélio/patologia , Regulação Neoplásica da Expressão Gênica , Mesoderma/patologia , MicroRNAs/metabolismo , Osteossarcoma/genética , Osteossarcoma/patologia , Fator de Transcrição PAX3/metabolismo , Proteínas Proto-Oncogênicas c-met/metabolismo , Apoptose/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Humanos , MicroRNAs/genética , Metástase Neoplásica , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
5.
Yi Chuan ; 40(9): 749-757, 2018 Sep 20.
Artigo em Chinês | MEDLINE | ID: mdl-30369478

RESUMO

Non-homologous end-joining (NHEJ) is the predominant DNA double-strand break (DSB) repair pathway in mammalian cells. It inhibits the efficiency of homologous recombination (HR) by competing for DSB targets. To improve the efficiency of HR in porcine fetal fibroblasts (PFFs), several RNA interference (RNAi) systems were designed to knockdown NHEJ key molecules, such as polynucleotide kinase/phosphatase (PNKP), DNA ligase IV (LIG4) and NHEJ1. The results show that siRNA significantly knocked down LIG4, PNKP and NHEJ1 expression. Suppression of PNKP dramatically increased the efficiency of single-strand annealing (SSA), double-strand DNA (dsDNA) and single-strand DNA (ssODN) mediated homology-directed repair (HDR) by 55.7%, 37.4% and 73.1% after transfected with the SSA-GFP reporter, HDR-GFP system or ssODN-GFP system, respectively; whereas knockdown of LIG4 and NHEJ1 repair factors significantly increased dsDNA or ssODN-mediated HDR efficiency by 37.5% and 76.9%, respectively.


Assuntos
Reparo do DNA por Junção de Extremidades , Recombinação Homóloga , Interferência de RNA , Suínos/genética , Animais , DNA Ligase Dependente de ATP/genética , DNA Ligase Dependente de ATP/metabolismo , DNA de Cadeia Simples/genética , DNA de Cadeia Simples/metabolismo , Feminino , Fibroblastos/metabolismo , Técnicas de Silenciamento de Genes , Masculino , Reparo de DNA por Recombinação , Suínos/embriologia , Suínos/metabolismo
6.
Pain Res Manag ; 2018: 2010129, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30651899

RESUMO

At present, there are many constantly updated guidelines and consensuses on the diagnosis and treatment of osteoarthritis both at home and abroad. The recommendations established using methods of evidence-based medicine has experienced strict research on controlling bias and promoting reproduction rate. As a result, the previous evidence was reevaluated, and a lot of changes were provoked in the diagnosis and treatment concept of osteoarthritis. However, several methods not recommended by foreign guidelines are still in use in the current clinical practice in China. On the one hand, Chinese experts have not reached extensive consensus on whether it is necessary to make changes according to foreign guidelines. On the other hand, almost all the current relevant guidelines are on osteoarthritis, but the lesions around knee joints which, as a whole, bear the largest weight in human body, cannot be ignored. For this purpose, Chinese Association for the Study of Pain (CASP) organized some leading experts to formulate this Chinese Pain Specialist Consensus on the diagnosis and treatment of degenerative knee osteoarthritis (DKOA) in combination with the guidelines in foreign countries and the expert experience of clinical practice in China. The consensus, which includes the definition, pathophysiology, epidemiology, clinical manifestation, diagnostic criteria, and treatments of DKOA, is intended to be used by first-line doctors, including pain physicians to manage patients with DKOA.


Assuntos
Articulação do Joelho/patologia , Osteoartrite do Joelho/diagnóstico , Osteoartrite do Joelho/terapia , Medicina Baseada em Evidências , Humanos , Osteoartrite do Joelho/patologia , Guias de Prática Clínica como Assunto
7.
Yi Chuan ; 39(10): 930-938, 2017 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-29070488

RESUMO

To obtain an ideal transfection efficiency of porcine fetal fibroblasts, fluorescence activated cell sorting (FACS) was used to optimize parameters for transfection of porcine fetal fibroblasts (PFFs) with ECM? 830, NEPA 21 and Nucleofector? 2b in different conditions such as electroporation parameters, plasmid dosages and topological structures. The results show that the optimum poring pulse parameter of NEPA 21 is voltage 200 V, continuous 3 ms, interval 50 ms, 3 times, voltage attenuation range of 10%; and the transfection efficiency of Nucleofector? 2b is highest under U-023 program. Under the optimum conditions, FACS analysis demonstrates that Nucleofector? 2b and ECM? 830 have the highest transfection efficiency when transfecting 10 µg supercoiled plasmids into PFFs, and 8 µg for NEPA 21. Supercoiled plasmids show higher transfection efficiencies than linearized plasmids. Moreover, Nucleofector? 2b has the highest transfection efficiency among the three electroporation instruments. This study paves the way to generate transgenic or gene editing pigs with high efficiency.


Assuntos
Eletroporação , Plasmídeos , Transfecção , Animais , Animais Geneticamente Modificados , Fibroblastos/metabolismo , Suínos
8.
Yi Chuan ; 39(2): 98-109, 2017 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-28242597

RESUMO

The traditional transgenic technologies, such as embryo microinjection, transposon-mediated integration, or lentiviral transfection, usually result in random insertions of the foreign DNA into the host genome, which could have various disadvantages in the establishment of transgenic animals. Therefore, a strategy for site-specific integration of a transgene is needed to generate genetically modified animals with accurate and identical genotypes. However, the efficiency for site-specific integration of transgene is very low, which is mainly caused by two issues. The first one is the low efficiency of inducing double-strand break (DSB) at the target site of host genome in the initial process. The second one is the low efficiency of homologous recombination repair (HDR) between the target site and the donor plasmid carrying homologous arm and foreign genes. HDR is the most common mechanism for site-specific integration of a transgene. DSBs can stimulate DNA repair mainly by two competitive mechanisms, HDR and nonhomologous end joining (NHEJ). Hence, activation of HDR or inhibition of NHEJ can promote the HDR in the integration processes, thereby optimizing a specific targeting of the transgene. In this review, we summarize the recent advances in strategies for improving the site-specific integration of foreign transgene in transgenic technologies.


Assuntos
Reparo de DNA por Recombinação , Transgenes , Animais , Animais Geneticamente Modificados , Quebras de DNA de Cadeia Dupla
9.
Biomed Res Int ; 2016: 5902678, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27672656

RESUMO

Recently, numerous studies indicate that H19 plays a key role in tumorigenesis, but the results have been disputed, especially in the aspects of tumor progression and metastasis. Therefore, we performed this meta-analysis to systematically summarize the relationship between H19 and cancers. We searched PubMed, the Cochrane Library, CNKI, and Chinese Wan Fang to identify eligible studies. Odds ratios and 95% confidence intervals were calculated to assess the effect size. A total of 13 studies were enrolled in this meta-analysis, which was performed by Revman5.3 and Stata11.0 software. Our meta-analysis showed that the expression of H19 was associated with distant metastasis in nongastrointestinal tumors (OR = 3.85, 95% CI = 1.31-11.36, P = 0.01) and, in gastrointestinal tumors (OR = 0.34, 95% CI = 0.15-0.78, P = 0.01), lymph node metastasis (OR = 2.04, 95% CI = 1.19-3.48, P = 0.009). Moreover, in gastric cancer, H19 expression was significantly related to histological grade (OR = 0.50, 95% CI = 0.29-0.86, P = 0.01), TNM stage (OR = 0.19, 95% CI = 0.11-0.33, P < 0.01), and tumor invasion depth (OR = 0.11, 95% CI = 0.04-0.27, P < 0.01). Therefore, H19 could serve as a potential marker for progression and metastasis evaluation of cancers.

10.
Asian Pac J Trop Med ; 8(2): 137-41, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25902028

RESUMO

OBJECTIVE: To evaluate the efficacy of allicin combined with cyclophosphamide on neuroblastoma (NB)-bearing mice and explore the immunological mechanism in it. METHODS: A total of 30 NB-bearing mice were equally randomized into model group, cyclophosphamide group and combined therapy group, 10 nudemice were set as normal saline (NS) group. Cyclophosphamide group and combined therapy group were weekly injected with 60 mg/kg cyclophosphamide for four weeks; besides, combined therapy group was given with allicin (10 mg/kg/d) by gastric perfusion for 4 weeks; model group and NS group were given with the same volume of NS. Serum VEGF content was detected by ELISA pre-treating (0 d) and on the 3rd d, 14th d and 28th d; on 29th d, all mice were sacrificed and the tumor, liver, spleen and thymic tissues were weighted. Tumors were made into paraffin section for detecting tumor cell apoptosis and proliferation by TUNEL and BrdU method, respectively. Survival curves were drawn by Kaplan-Meier method. RESULTS: After treatment, both treatment groups relieved on viscera indexes, VEGF level, T cell subsets distribution and tumor growth and each index of combined therapy group was better than cyclophosphamide group (P<0.05 or 0.01); only combined therapy group could significantly increase the lifetime of NB-bearing mice (µ (2)=5.667, P=0.017). CONCLUSIONS: Allicin can improve T cell subsets distribution and inhibit VEGF expression through its immunomodulatory activity, thereby improve the efficiency on NB in coordination with cyclophosphamide.

11.
Mycoses ; 57(9): 560-4, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24697872

RESUMO

Hyperkeratotic-type tinea pedis is chronic and recalcitrant to topical antifungal agents. Some topical antifungal agents are effective; however, long duration of therapy is required, which often reduce the treatment compliance of patients. To seek for short period therapy of hyperkeratotic type tinea pedis, in this study, we observed the efficacy and safety of treatment of topical terbinafine and 10% urea ointment combined oral terbinafine. Participants with hyperkeratotic type tinea pedis were randomly assigned to two groups. Patients in group I were treated with oral terbinafine for 2 weeks and topical terbinafine and 10% urea ointment for 4 weeks, whereas in group II, only the above topical agents were applied for 12 weeks. Clinical improvement rates and fungal eradication rates were compared between the two groups at 24 weeks after the initiation of treatment. The group I had stopped the topical therapy 8 weeks earlier than group II. There were no significant differences in mycological eradication rates and clinical improvement rates between the two groups, besides, no major side effects were noted in both groups. The short combination therapy with oral terbinafine was effective and safe; it should be a valuable option for patients with hyperkeratotic type tinea pedis.


Assuntos
Antifúngicos/administração & dosagem , Naftalenos/administração & dosagem , Tinha dos Pés/tratamento farmacológico , Ureia/administração & dosagem , Administração Oral , Administração Tópica , Adulto , Idoso , Antifúngicos/efeitos adversos , Quimioterapia Combinada/efeitos adversos , Quimioterapia Combinada/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Naftalenos/efeitos adversos , Pomadas/administração & dosagem , Estudos Prospectivos , Terbinafina , Resultado do Tratamento , Ureia/efeitos adversos , Adulto Jovem
12.
J Pediatr Surg ; 47(5): 1038-42, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22595598

RESUMO

PURPOSE: The purpose of this study is to present the management of idiopathic megaduodenum in children. METHODS: A retrospective analysis of 4 cases of megaduodenum admitted from 2005 to 2011 was performed evaluating clinical features, radiologic data, treatment, pathologic findings, and prognosis. The corresponding literature was reviewed. RESULTS: The diagnosis of nonobstructive megaduodenum was confirmed by upper gastrointestinal contrast study, ultrasonography, and exploratory laparotomy. Treatment consisted of either tapering duodenoplasty with pylorus division and closure of the proximal stump plus Roux-en-Y gastrojejunostomy or tapering duodenoplasty with closure of the proximal stump and end-to-side gastrojejunostomy. On pathologic evaluation, neural and vascular structures appeared normal in all sections. All symptoms, including diarrhea, bloating, vomiting, and nausea, had resolved on follow-up, and all patients experienced rapid weight gain after their operation. CONCLUSIONS: Idiopathic megaduodenum without organic obstruction is a rare clinical condition. Massive dilatation confined to the duodenum was shown by upper gastrointestinal contrast studies and ultrasonography and can also be identified on antenatal ultrasonography. In children with megaduodenum, satisfactory results can be obtained by tapering duodenoplasty with proximal stump closure and gastrojejunostomy with either Roux-en-Y or end-to-side anastomosis.


Assuntos
Duodeno/cirurgia , Doenças Fetais/cirurgia , Derivação Gástrica/métodos , Criança , Pré-Escolar , Duodeno/anormalidades , Feminino , Doenças Fetais/diagnóstico , Doenças Fetais/etiologia , Seguimentos , Humanos , Lactente , Masculino , Estudos Retrospectivos , Resultado do Tratamento , Bexiga Urinária/anormalidades , Bexiga Urinária/cirurgia
13.
Zhonghua Yi Xue Za Zhi ; 92(7): 448-51, 2012 Feb 21.
Artigo em Chinês | MEDLINE | ID: mdl-22490963

RESUMO

OBJECTIVE: To analyze the profiles of pressure pain threshold (PPT) and pressure pain tolerance (PTO) of healthy undergraduates so as to establish the normal reference ranges of PPT and PTO. METHODS: The levels of PPT and PTO were measured at lateral brachioradialis of lateral elbow joint with a pressure algometer in 113 healthy undergraduates. Then the influencing factors of tenderness thresholds were analyzed and the normal reference ranges of PPT and PTO established. RESULTS: The females exhibited a lower PPT than the males (P < 0.01). And the gender differences of PTO were statistically insignificant (P > 0.05). Meanwhile, there was no significant difference in either PPT or PTO between the right and left sides (P > 0.05). The normal reference ranges (kg/cm(2)) of PPT and PTO were obtained at the measuring spot of all subjects. As a result, the ranges of PPT were 1.19 - 4.63 in the males and 0.37 - 3.63 in the females. And the range of PTO was 1.80 - 8.50 in all subjects. CONCLUSION: This survey establishes the normal reference ranges of tenderness thresholds at the specific measuring spot for some specific population. These ranges may serve as a reference for the sensitivity of individual tenderness safely, accurately and simply.


Assuntos
Limiar da Dor , Adulto , Feminino , Humanos , Masculino , Medição da Dor , Valores de Referência , Adulto Jovem
14.
Dalton Trans ; 40(4): 779-81, 2011 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-21152480

RESUMO

Three anionic polyoxometalates contain catalytically active vanadium(IV) centers and peripheral metal sites of Mn(2+), Co(2+) or Ni(2+) cations, which synergistically affect the catalytic selectivities of unprecedented oxidative cyclization of acetylacetone to form two interesting multifunctional furan derivatives.

15.
Dalton Trans ; 39(14): 3396-9, 2010 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-20379532

RESUMO

The reaction of [Na(12)(H(2)O)(38)][WZn{M(H(2)O)}(2)(ZnW(9)O(34))(2)].3H(2)O (M = Mn, Co) and Co(NO(3))(2).6H(2)O in water at 80 degrees C afforded red crystals of [Co(H(2)O)(6)](2){[Co(H(2)O)(4)](2)[Co(H(2)O)(5)](2)WZn[Co(H(2)O)](2)(ZnW(9)O(34))(2)}.10H(2)O (1), which has been characterized by TGA, IR, PXRD, element analysis and single crystal X-ray diffraction. The polyanion [WZn{Co(H(2)O)}(2)(ZnW(9)O(34))(2)](12-) in is double-linked by cobalt(ii) cations to extend into a 1D anionic polymeric chain of {[Co(H(2)O)(4)](2)[Co(H(2)O)(5)](2)WZn[Co(H(2)O)](2)(ZnW(9)O(34))(2)}(4-) with partial free cobalt cations. The redox active cobalt(ii) sites made compound an excellent catalyst for the oxidation of styrene with high styrene conversion (up to 96%) and high benzaldehyde selectivity (up to 99%).


Assuntos
Benzaldeídos/química , Estireno/química , Compostos de Tungstênio/química , Benzaldeídos/síntese química , Catálise , Cobalto/química , Cristalografia por Raios X , Conformação Molecular , Oxirredução
16.
Am J Emerg Med ; 27(8): 935-41, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19857411

RESUMO

The advantage of vasopressin over epinephrine in the treatment of cardiac arrest (CA) is still being debated, and it is not clear whether a high dose of vasopressin is beneficial or detrimental during or after cardiopulmonary resuscitation (CPR) in a rat model of CA. In this study, asphyxial CA was induced in 40 male Sprague-Dawley rats. After 10 minutes of asphyxia, CPR was initiated; and the effects of different doses of vasopressin (low dose, 0.4 U/kg; medium dose, 0.8 U/kg; and high dose, 2.4 U/kg; intravenous; n = 10 in each group) and a saline control (isotonic sodium chloride solution, 1 mL, intravenous) were compared. Outcome measures included the rate of restoration of spontaneous circulation (ROSC) and changes of hemodynamic and respiratory variables after ROSC. The rates of ROSC were 1 of 10 in the saline group and 8 of 10 in each of the 3 vasopressin groups. There were no differences in mean aortic pressure or changes of respiratory function after CPR among the vasopressin groups. However, the heart rate was lower in the high-dose vasopressin group than in the low- and medium-dose groups. These findings indicate that different doses of vasopressin result in a similar outcome of CPR, with no additional benefits afforded by a high dose of vasopressin during or after CPR, in a rat model of asphyxial CA. The mechanism and physiologic significance of the relative bradycardia that occurred in the high-dose vasopressin group are currently unknown and require further investigation.


Assuntos
Asfixia/complicações , Parada Cardíaca/tratamento farmacológico , Vasoconstritores/farmacologia , Vasopressinas/farmacologia , Animais , Reanimação Cardiopulmonar , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Parada Cardíaca/etiologia , Masculino , Ratos , Ratos Sprague-Dawley , Vasoconstritores/administração & dosagem , Vasopressinas/administração & dosagem
17.
Huan Jing Ke Xue ; 30(1): 172-7, 2009 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-19353877

RESUMO

Sorption behavior of phosphorus on sediment samples taken from Inner Mongolia reach of the Yellow River in 6 different geographical sites was determined in laboratory, and the correlation between chemical-physical properties of the sediments and their phosphorus sorption characteristics was analyzed. The maximum sorption capacities were 43.64-85.46 mg/kg. The zero equilibrium P concentration (EPC0) of the sediments ranged from 0.0003 to 0.0198 mg/L, which was positively correlated to the contents of native adsorbed P (NAP). The sediments played a dual role of sink and source of P at different geographical sites. Sediments from Shizuishan, Wulateqianqi and Qingshuihe along the Yellow River were sources of phosphorus, while sediments from Wuhai, Linhe and Baotou cross sections were sinks of phosphorus, at the typical P concentrations of the river water. However, both the sorption and desorption capacities were low at the conditions tested here. The Langmuir sorption constant (k) was positively correlated to the cation exchange capacity (CEC), organic matter and clay mineral content, but negatively correlated to the particle size of the sediments. The maximum sorption capacity of phosphorus was remarkably correlated to the active Fe content. Active Fe played a key role in holding phosphorus on the sediments.


Assuntos
Água Doce/análise , Sedimentos Geológicos/química , Fósforo/análise , Poluentes Químicos da Água/análise , Adsorção , China , Monitoramento Ambiental , Rios , Poluentes Químicos da Água/química
19.
Huan Jing Ke Xue ; 29(8): 2137-42, 2008 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-18839562

RESUMO

The equilibrium phosphate concentration (EPC0) of the Yellow River bed sediments has been measured, which was used to predict whether bed sediments are acting as a source or sink of soluble reactive phosphate (SRP). The modified Langmuir isotherm equation was used to describe phosphate (P) sorption on the Yellow River sediments. The maximum P sorption capacity (PAC) and P-binding energy constant (k) were obtained by the modified Langmuir isotherm model. Native adsorbed exchangeable phosphorus (NAP), the EPC0, and partitioning coefficients (Kp) were subsequently calculated by the corresponding formulae. The influence of pH values and ion strength were evaluated. All the EPC0 s are higher than the P concentration in the overlying water, indicating a potential source of phosphate from the sediments. PAC is linearly related to the contents of TOC of the sediment. The sorption capacity of P increased rapidly with pH below 6.0, and then reached a plateau between pH 6.0 to 9.7, and finally maintained at a slightly higher level from pH 9.7 to 12.0.The adsorption of P by the sediment decreased with the increase in Ca2+ ionic strength.


Assuntos
Água Doce/análise , Sedimentos Geológicos/química , Fosfatos/análise , Poluentes Químicos da Água/análise , Adsorção , China , Monitoramento Ambiental , Água Doce/química , Fosfatos/química , Rios , Estações do Ano , Poluentes Químicos da Água/química
20.
Zhonghua Yi Xue Za Zhi ; 86(29): 2041-3, 2006 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-17064548

RESUMO

OBJECTIVE: To investigate the effects of music on propofol target-controlled infusion (TCI) in patients during gynecological surgery under intravertebral anesthesia. METHODS: One hundred and ten patients scheduled for gynecological surgery under intravertebral anesthesia were randomly assigned to two groups. The music group patients were asked to determine music and its sound volume preoperatively, which were played from 6 min before the surgery to the end. A loading dose of 1 microg/kg fentanyl and 0.5 mg/kg propofol was administered intravenously to all the patients 5 min prior to operation. At the beginning of surgery, the TCI of propofol was initiated at propofol target controlled concentration of 2 microg/ml, which was modulated at a grade of 0.2 microg/ml every 10 min to maintain an observer's assessment of alertness/sedation (OAA/S) score of 3 intraoperatively. Induction time of sedation, intraoperative amount of propofol and patient's satisfaction of sedation were assessed. RESULTS: (1) The music group patients had a significant shorter induction time of sedation (12 min +/- 12 min) than the controls (18 min +/- 12 min) (P < 0.01). (2) The music group patients had a significant less propofol target-controlled concentration (1.6 microg/ml +/- 0.3 microg/ml) and delivers (171 mg +/- 98 mg) than the controls (2.4 microg/ml +/- 0.4 microg/ml and 251 mg +/- 92 mg) (both P < 0.01). (3) The music group patients had a significant higher satisfaction (9.7 +/- 0.6) than the controls (8.1 +/- 1.0) (P < 0.01). CONCLUSION: Music has perfect sedative effects in patients undergoing gynecological surgery under intravertebral anesthesia.


Assuntos
Sedação Consciente/métodos , Procedimentos Cirúrgicos em Ginecologia/métodos , Musicoterapia , Adulto , Anestesia/métodos , Sistemas de Liberação de Medicamentos , Feminino , Humanos , Pessoa de Meia-Idade
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