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1.
J Tradit Chin Med ; 40(4): 674-682, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32744035

RESUMO

OBJECTIVE: To evaluate the effects of Qizhukangxian granules (QG) on idiopathic pulmonary fibrosis (IPF). METHODS: This is a randomized, double blind, placebo-controlled and multicenter clinical pilot trial. Six medical centers in Tianjin, China, participated in the study. A total of 120 IPF patients were enrolled and randomized into two groups, with 60 patients in each group. The treatment group was treated with QG, while the control group received a Qizhukangxian placebo. The pharmacological treatment lasted for 48 weeks from the enrollment date. The indexes of patients were recorded on the admission day and at the end of the 24th and 48th weeks. Data were analyzed to study the effects of QG; forced vital capacity, change in forced vital capacity and maximal 6-min walk test (6MWT) distance were the primary endpoints. Secondary endpoints were percentage of patients with episodes of acute exacerbation of IPF, pulmonary function, changes in pulse oxygen saturation during the 6MWT, dyspnea score, St. George's respiratory questionnaire score, arterial blood gas analyses and the total Traditional Chinese Medicine symptom pattern score. RESULTS: After 24 weeks of treatment, QG showed greater efficacy than the placebo in certain parameters, including the dyspnea score, Traditional Chinese Medicine symptom pattern score and some indicators in the St. George's respiratory questionnaire score. Analysis of the indexes obtained from all patients at the end of the 48th week showed that the therapeutic effects in the treatment group were significantly better than those in the control group because remarkable differences were observed in most of the primary and secondary endpoints between the two groups, except for the maximal distance of the 6MWT and arterial blood gas analyses. No adverse reaction was observed in either group during the 48-week trial treatment period. CONCLUSION: QG could effectively treat IPF patients by ameliorating pulmonary function, improving the quality of life and lowering the percentage of acute exacerbations.

2.
BMC Musculoskelet Disord ; 21(1): 408, 2020 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-32600299

RESUMO

BACKGROUND: Fixing a tibial eminence fracture with a tri-pulley is a new technique. The purpose of this study was to present the early clinical outcome of arthroscopic tri-pulley suture fixation for tibial eminence fractures in children. METHODS: Twenty-one pediatric patients with type II or type III anterior tibial eminence fractures were included in this retrospective study. All Patients underwent surgical fixation by tri-pulley technology and were followed up for at least 24 months. They were evaluated preoperatively and postoperatively by physical, X-ray, and computed tomography (CT) examination and subjectively with the International Knee Documentation Committee (IKDC), and Lysholm questionnaires. RESULTS: The patients included 12 males and 9 females; mean age, 12.5 years (range, 8 ~ 16 years). They were followed-up for a median of 27 months (range, 24 ~ 39 months). We did not find post-operative instability in any of the patients by physical examination. The KT-2000 difference of both knees decreased from 9.3 ± 1.2 mm preoperatively to 2.6 ± 0.8 mm 24 months postoperatively (P < 0.001); the IKDC subjective knee evaluation score improved from 43.1 ± 13.2 preoperatively to 83.8 ± 6.3 postoperatively (P < 0.001); and Lysholm improved from 48.3 ± 6.21 to 87.1 ± 9.8 (P < 0.001). No unhealed fractures or epiphyseal damage were reported in the postoperative X-ray and CT. CONCLUSIONS: Arthroscopic tri-pulley fixation technology may provide a suitable technique for repair of tibial eminence fractures in skeletally immature patients. LEVEL OF EVIDENCE: Case series; Level of evidence IV.

3.
Inorg Chem ; 2020 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-32614177

RESUMO

Sr5Ga8O3S14, which is a novel nonlinear optical oxysulfide, was discovered via solid-state reaction. The compound crystallizes in noncentrosymmetric space group P21212 (No. 18) of the orthorhombic system. The crystal structure is derived from the melilite structure and composed of anionic [Formula: see text] layers intercalated by Sr2+ cations. The anionic layers are constructed by [GaOS3]5- and [GaS4]5- tetrahedra via either corner sharing or edge sharing, while typical melilite only features edge-sharing mode. Because of the existence of electronegative O atoms, it possesses a large band gap (3.9 eV) with a wide transparent region of 0.3-13.4 µm. It exhibits second-harmonic-generation response, which is 0.8 times that of the reference AgGaS2 (AGS) in the particle size range of 20-41 µm under laser irradiation at a wavelength of 2.09 µm laser. Density functional theory calculations indicate that the band edges are mainly composed of the Ga-S bond orbitals, with little participation of O 2p near the valence band maximum.

4.
Gen Comp Endocrinol ; 298: 113551, 2020 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-32687936

RESUMO

The yellowtail clownfish (Amphiprion clarkii) is a hermaphrodite fish, whose sex differentiation and gonad development are closely related to its social status. The kisspeptin/KissR system is regarded as a key factor mediating social stress on reproductive regulation. In order to understand the effects of social rank stress on the yellowtail clownfish gonadal differentiation, full-length cDNAs of two paralogous genes encoding kisspeptin (kiss1 and kiss2) and KissR (kissr2 and kissr3) were cloned and characterized. The results of real-time PCR showed that kiss1 was primarily expressed in the hypothalamus, and kiss2/kissr2 were abundantly expressed in the liver, while kissr3 was almost exclusively concentrated in the cerebellum and pituitary. Moreover, both Kiss1-10 and Kiss2-10 peptides could initiate downstream signaling pathways by interacting with cognate receptors expressed in eukaryotic cells. Among the three social status groups, the mRNA levels of kiss2 in the hypothalamus and pituitary as well as kissr2 in the pituitary were significantly higher in subordinate individuals (nonbreeders) than dominate individuals (females and males); while the mRNA levels of kissr3 in the hypothalamus and gonad were low in subordinate individuals. Furthermore, the plasma estradiol (E2) and testosterone (T) levels were higher in subordinate than dominate individuals. This study shows that kiss2 is involved in the regulation of social stress on the gonad development in the yellowtail clownfish, but not kiss1.

5.
Nat Biomed Eng ; 2020 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-32632226

RESUMO

Patients with kidney failure commonly require dialysis to remove nitrogenous wastes and to reduce burden to the kidney. Here, we show that a bacterial cocktail orally delivered in animals with kidney injury can metabolize blood nitrogenous waste products before they diffuse through the intestinal mucosal barrier. The microbial cocktail consists of three strains of bacteria isolated from faecal microbiota that metabolize urea and creatinine into amino acids, and is encapsulated in calcium alginate microspheres coated with a polydopamine layer that is selectively permeable to small-molecule nitrogenous wastes. In murine models of acute kidney injury and chronic kidney failure, and in porcine kidney failure models, the encapsulated microbial cocktail significantly reduced urea and creatinine concentrations in blood, and did not lead to any adverse effects.

6.
BMC Plant Biol ; 20(1): 351, 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32713350

RESUMO

BACKGROUND: N6-Methyladenosine (m6A) is the most widespread RNA modification that plays roles in the regulation of genes and genome stability. YT521-B homology (YTH) domain-containing RNA-binding proteins are important RNA binding proteins that affect the fate of m6A-containing RNA by binding m6A. Little is known about the YTH genes in common wheat (Triticum aestivum L.), one of the most important crops for humans. RESULTS: A total of 39 TaYTH genes were identified in common wheat, which are comprised of 13 homologous triads, and could be mapped in 18 out of the 21 chromosomes. A phylogenetic analysis revealed that the TaYTHs could be divided into two groups: YTHDF (TaDF) and YTHDC (TaDC). The TaYTHs in the same group share similar motif distributions and domain organizations, which indicates functional similarity between the closely related TaYTHs. The TaDF proteins share only one domain, which is the YTH domain. In contrast, the TaDCs possess three C3H1-type zinc finger repeats at their N-termini in addition to their central YTH domain. In TaDFs, the predicated aromatic cage pocket that binds the methylysine residue of m6A is composed of tryptophan, tryptophan, and tryptophan (WWW). In contrast, the aromatic cage pocket in the TaDCs is composed of tryptophan, tryptophan, and tyrosine (WWY). In addition to the general aspartic acid or asparagine residue used to form a hydrogen bond with N1 of m6A, histidine might be utilized in some TaDFb proteins. An analysis of the expression using both online RNA-Seq data and quantitative real-time PCR verification revealed that the TaDFa and TaDFb genes are highly expressed in various tissues/organs compared with that of TaDFcs and TaDCs. In addition, the expression of the TaYTH genes is changed in response to various abiotic stresses. CONCLUSIONS: In this study, we identified 39 TaYTH genes from common wheat. The phylogenetic structure, chromosome distribution, and patterns of expression of these genes and their protein structures were analyzed. Our results provide a foundation for the functional analysis of TaYTHs in the future.

7.
Artigo em Inglês | MEDLINE | ID: mdl-32632695

RESUMO

In the context of the rapid development of the Belt and Road (B&R) Initiative, the continuous transfer of Sino-US trade to the B&R countries is an important means to mitigate the threat of Sino-US trade, and the environmental impact of this transfer should be considered, so as to provide a scientific basis for China's policy formulation about achieving this possible trade transfer with minimized environmental impacts. This study proposes a multiregional input-output model and analyzes the impact on carbon dioxide (CO2) emissions of transferring the Sino-US trade to the B&R countries for two types of scenarios. The results show the following: (1) A transfer of either the import trade or the export trade increases global and Chinese CO2 emissions by 81.76 Mt and 24.84 Mt, respectively. When both the import trade and export trade are transferred, the increases in CO2 emissions are only 0.22% and 0.26%, respectively. (2) Globally, the changes in international trade-embodied CO2 emissions are responsible for most of the global emission changes, especially the CO2 emissions exported from Russia, India, and many Southeast Asian countries to China. (3) Different from the impact on global emissions, the increases in Chinese domestic production-based CO2 emissions influence China's total CO2 emissions. Due to the imported CO2 emissions, the consumption-based CO2 emissions are affected to a greater degree and increase by 70.30 Mt, accounting for only 0.86% of the CO2 emissions in 2015. Finally, some policy implications are proposed.

8.
Artigo em Inglês | MEDLINE | ID: mdl-32648640

RESUMO

Multi-component MOFs contain multiple sets of unique and hierarchical pores, with different functions for different applications, distributed in their inter-linked domains. Herein, we report the construction of a class of precisely aligned flexible-on-rigid hybrid-phase MOFs with unique rods-on-octahedron morphology. We demonstrated that hybrid-phase MOFs can be constructed based on two prerequisites: the partially matched topology at the interface of the two frameworks, and the structural flexibility of MOFs with acs topology, which can compensate for the differences in lattice parameters. Furthermore, we achieved domain selective loading of multiple guest molecules into the hybrid-phase MOF, as observed by scanning transmission electron microscopy-energy dispersive X-ray spectrometry elemental mapping. Most importantly, we successfully applied the constructed hybrid-phase MOF to develop a dual-drug delivery system with controllable loading ratio and release kinetics.

9.
Yi Chuan ; 42(7): 641-656, 2020 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-32694104

RESUMO

Gene-editing technology can artificially modify genetic material of targeted loci by precise insertion, deletion, or replacement in the genomic DNA. In recent years, with the developments of zinc-finger endonuclease (ZFN), transcription activator-like effector nuclease (TALEN), clustered regularly interspaced short palindromic repeats/CRISPR- associated protein 9 (CRISPR/Cas9) technologies, such precise modifications of the animal genomes have become possible. Although gene-editing tools, such as CRISPR/Cas9, can efficiently generate double-strand breaks (DSBs) in mammalian cells, the homology-directed repair (HDR) mediated knock-in (KI) efficiency is extremely low. In this review, we briefly describe the current development of gene-editing tools and summarize the recent strategies to enhance the CRISPR/Cas9- mediated KI efficiency, which will provide a reference for the generation of human disease models, research on gene therapy and livestock genetic improvement.

10.
ACS Nano ; 2020 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-32658459

RESUMO

Metal carbonyl complexes can readily liberate carbon monoxide (CO) in response to activation stimulus. However, applicability of metal carbonyl complexes is limited because they are unstable under natural ambient conditions of moisture and oxygen. Reported here is the rational design of iron carbonyl complex delivery nanosystem for the improvement of cancer therapy. We demonstrated that iron pentacarbonyl (Fe(CO)5) can be encapsulated into the cavity of Au nanocage under an oxygen free atmosphere and then controllably form iron oxide on the surface of Au nanocage under aerobic condition. The formation of iron oxide efficiently avoids the leakage and oxidation of the caged Fe(CO)5. The resulting nanomaterial exhibits excellent safety, biocompatibility and stability, which can be specifically activated under near-infrared (NIR) irradiation within the tumor environment to generate CO and iron. The released CO causes the damage of mitochondria and subsequent initiation of autophagy. More importantly, during autophagy, the nanomaterial that contains iron and iron oxide can accumulate into the autolysosome and result into the destruction of autolysosome. The produced CO and iron show excellent synergistic effects in cancer cells.

11.
Anal Chem ; 2020 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-32600030

RESUMO

Acute myeloid leukemia (AML) is a heterogeneous disease, and there are critical interests in detecting multiple biomarkers as a single biomarker detection cannot reflect the exact phase of the disease. Exosomes derived from different types of AML cells contain respective combinations of cluster of differentiation (CD) markers that may be used to guide the molecular typing of AML in the clinic. Here, aiming to build more precise molecular typing of AML, we demonstrate multiplex immuno-PCR (mI-PCR) assay for simultaneous detection of multiple surface CDs on exosomes of AML via capillary electrophoresis with laser-induced fluorescence (CE-LIF). This method comprises of four steps: (1) chemical attachment of reporter DNA sequence to the specific detection antibodies, (2) binding of the detection antibodies to their targets on the exosomes, (3) DNA amplification of the reporter DNA, and (4) capillary electrophoresis analysis of the PCR products. With the method, we first realized simultaneous detection of five target CD molecules (CD9, CD34, c-Kit/CD117, CD123, and FLT-3/CD135) on leukemia cell-derived exosomes with high detection sensitivity. The limit of detection (LOD) and limit of quantification (LOQ) are 2.41 ± 0.04 particles/µL and 8.02 ± 0.16 particles/µL, respectively, for leukemia cell-derived exosomes. This mI-PCR is found sensitive enough to detect picogram (10-12) levels of protein concentrations with high recovery (95%) in spiked serum sample experiments. We thus anticipate that the proposed method is promising in sensitive detection of multitargets to assist in the precise molecular typing of many complex diseases.

12.
Water Res ; 183: 116046, 2020 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-32629180

RESUMO

We recently reported (Sobhani et al., 2020) that when a confocal Raman microscope imaged a nanoplastic with the diameter of 100 nm, the imaging lateral size was 300-400 nm, due to the diffraction limit of the laser spot. In this study, we examine the lateral intensity distribution of the Raman signal emitted by nanoplastics (diameters ranging ∼30-600 nm) within the excitation laser spot. We find that the Raman emission intensity, similar to the excitation power density distributed within a laser spot, also follows a lateral Gaussian distribution. To image and visualise individual nanoplastics, we (i) decrease the mapping pixel size, in a hope to generate an image with high-resolution and simultaneously to pick up items from the "blind point". We can then either (ii) offset the colour to intentionally image only the high-intensity portion of the Raman signal (emitted from the centre of the laser spot), to localise the exact position of the nanoplastic; or (iii) categorise the imaged nanoplastics to different groups via their Raman intensity, to simultaneously and separately visualise large nanoplastics/strong Raman signals, medium nanoplastics and small nanoplastics, in an effort to avoid the shielding and overlooking of weak signals. We (iv) also cross-check multi-images simultaneously mapped at two or three characteristic peaks via either a logic-OR or a logic-AND algorithm. Thus the imaging uncertainty can be significantly reduced from a statistical point of view.

13.
Biomed Res Int ; 2020: 5849123, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32596333

RESUMO

Members of genus Sphingopyxis are frequently found in diverse eco-environments worldwide and have been traditionally considered to play vital roles in the degradation of aromatic compounds. Over recent decades, many aromatic-degrading Sphingopyxis strains have been isolated and recorded, but little is known about their genetic nature related to aromatic compounds biodegradation. In this study, bacterial genomes of 19 Sphingopyxis strains were used for comparative analyses. Phylogeny showed an ambiguous relatedness between bacterial strains and their habitat specificity, while clustering based on Cluster of Orthologous Groups suggested the potential link of functional profile with substrate-specific traits. Pan-genome analysis revealed that 19 individuals were predicted to share 1,066 orthologous genes, indicating a high genetic homogeneity among Sphingopyxis strains. Notably, KEGG Automatic Annotation Server results suggested that most genes pertaining aromatic compounds biodegradation were predicted to be involved in benzoate, phenylalanine, and aminobenzoate metabolism. Among them, ß-ketoadipate biodegradation might be the main pathway in Sphingopyxis strains. Further inspection showed that a number of mobile genetic elements varied in Sphingopyxis genomes, and plasmid-mediated gene transfer coupled with prophage- and transposon-mediated rearrangements might play prominent roles in the evolution of bacterial genomes. Collectively, our findings presented that Sphingopyxis isolates might be the promising candidates for biodegradation of aromatic compounds in pollution sites.

14.
Chem Commun (Camb) ; 2020 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-32597434

RESUMO

New paracelsian-like beryllophosphates M4LiBe4P7O24 and their tetrahedra-substituted phases M4Li(Li3P)P7O24 (MLiP2O6, M = Cs, Rb) were designed and synthesized. Interestingly, two types of phases undergo a structural transformation, resulting from the radius and valence charge number of tetrahedral cations. Four crystals demonstrate potential deep-UV NLO properties such as short absorption edges (<190 nm) and phase-matching SHG response.

15.
J Diabetes Investig ; 2020 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-32593190

RESUMO

AIMS/INTRODUCTION: Chronic inflammation is an underlying feature of type 2 diabetes mellitus. Hypovitaminosis D is associated with type 2 diabetes mellitus, but whether it contributes to chronic inflammation is unclear. We examined the effects of vitamin D on various immune markers to evaluate its contribution to systemic inflammation in type 2 diabetes mellitus. MATERIALS AND METHODS: We retrospectively analyzed data from type 2 diabetes mellitus patients, people with prediabetes and control patients without diabetes (n = 9,746). Demographic and clinical variables were evaluated using descriptive statistics and generalized linear regression. A stratified analysis based on total serum vitamin D was also carried out. RESULTS: Neutrophil count was a significant predictor of 1,5-anhydroglucitol and glycated hemoglobin (HbA1c) in patients with prediabetes (1,5-anhydroglucitol: ß = -0.719, P < 0.001 and HbA1c: ß = -0.006, P = 0.002) and patients with diabetes (1,5-anhydroglucitol: ß = 0.207, P = 0.004 and HbA1c: ß = -0.067, P = 0.010). Lymphocyte count was a significant predictor of HbA1c in patients without diabetes (ß = 0.056, P < 0.001) and patients with prediabetes (ß = 0.038, P < 0.001). The neutrophil-to-lymphocyte ratio (NLR) was a significant predictor of HbA1c in patients without diabetes (ß = -0.001, P = 0.032). No immune markers differed significantly based on vitamin D level among patients without diabetes (P> 0.05 for all). Among patients with prediabetes, those who were vitamin D-deficient had the highest NLR (P = 0.040). Among patients with diabetes, those who were vitamin D-deficient had the highest neutrophil count (P = 0.001), lowest lymphocyte count (P = 0.016) and highest NLR (P < 0.001). CONCLUSIONS: The NLR is strongly influenced by serum vitamin D level. Given the high prevalence of hypovitaminosis D and elevated NLR among chronic disease patients and the elderly, our results suggest that clinical interpretation of NLR as a predictive marker of type 2 diabetes mellitus-related inflammation should consider vitamin D level, age and pre-existing morbidity.

16.
Sci China Life Sci ; 63(8): 1099-1102, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32557290
18.
Inorg Chem ; 2020 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-32558567

RESUMO

The development of new responsive smart materials has been highly desirable in the recent decade due to growing demand in our daily life, and extended viologen-based coordination polymers are regarded as proper and promising candidates for stimuli-responsive study. A tri(pyridinyl)pyridine viologen-based Kagome dual (kgd) topological coordination polymer, [Mn3Cl4(tpptb)2]·Cl2·(H2O)2, (tpptb = N,N',N″-tri(3-carboxybenzyl)-2,4,6-tri(pyridinium-4-yl)pyridine; 1) has been solvothermally synthesized, which can selectively respond to soft X-ray Al-Kα (λ = 8.357 Å) irradiation but not to UV light and hard X-rays of λ < 1.5418 Å at room temperature. Appealingly, 1 is very sensitive and convenient for the visual detection of various volatile amine vapors, especially ethylamine vapors at a low concentration of 100 ppm, and the vapochromic sample can be recovered after exposure in the air at room temperature. The sequence of amines in vapochromism could be rationalized by combined consideration of vapor pressure, the molecular size, and electron-donor ability of various amine molecules as well as the void spaces of 1. In addition, 1 exhibits an obvious hydrochromic transformation upon heating in the air and an anhydrous atmosphere. Combined XPS and EPR confirmed that these physical and chemical stimuli can cause electron transfer from electron-rich groups to quaternary nitrogen atoms of the ligand to generate charge-separated radicals, leading to soft X-ray-induced photochromic and selective vapochromic behavior of 1. Such behavior indicates that it will become a convenient, recyclable, and practical multifunctional material for chemical and environmental sensing. These results provide an effective avenue for the rational design and synthesis of multifunctional chromic materials for potential use in sensing devices.

19.
Sci Total Environ ; 741: 140445, 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32599407

RESUMO

The outbreak of coronavirus infectious disease-2019 (COVID-19) pneumonia raises the concerns of effective deactivation of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in medical wastewater by disinfectants. In this study, we evaluated the presence of SARS-CoV-2 viral RNA in septic tanks of Wuchang Cabin Hospital and found a striking high level of (0.5-18.7) × 103 copies/L after disinfection with sodium hypochlorite. Embedded viruses in stool particles might be released in septic tanks, behaving as a secondary source of SARS-CoV-2 and potentially contributing to its spread through drainage pipelines. Current recommended disinfection strategy (free chlorine ≥0.5 mg/L after at least 30 min suggested by World Health Organization; free chlorine above 6.5 mg/L after 1.5-h contact by China Centers for Disease Control and Prevention) needs to be reevaluated to completely remove SARS-CoV-2 viral RNA in non-centralized disinfection system and effectively deactivate SARS-CoV-2. The effluents showed negative results for SARS-CoV-2 viral RNA when overdosed with sodium hypochlorite but had high a level of disinfection by-product residuals, possessing significant ecological risks.

20.
Int J Syst Evol Microbiol ; 70(6): 3859-3864, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32501195

RESUMO

A novel bacterial strain, designated ysch24T, was isolated from a forest soil sample collected from the Cat Tien National Park, southern Vietnam. Cells were Gram-stain-negative, aerobic, gliding, filamentous or rod-shaped. The results of 16S rRNA gene analyses revealed that strain ysch24T belongs to the genus Chitinophaga, and was most closely related to Chitinophaga silvisoli GDMCC 1.1411T (97.4 %), followed by Chitinophaga oryziterrae JCM 16595T (97.3 %) and Chitinophaga sancti NBRC 15057T (96.9 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain ysch24T and closely related type strains were 72.0-74.0 % and 19.1-19.4 %, respectively. Major fatty acids were iso-C15 : 0, C16 : 1 ω5c and iso-C17 : 0 3-OH and the predominant respiratory quinone was menaquinone 7. Polar lipids consisted of phosphatidylethanolamine, four unidentified aminophospholipids, two unidentified phospholipids and four unidentified lipids. The genomic DNA G+C content was 45.6 mol%. The study clearly showed that strain ysch24T should represent a novel species of the genus Chitinophaga, for which the name Chitinophaga tropicalis sp. nov. is proposed. The type strain is ysch24T (=GDMCC 1.1355T=KACC 21527T).


Assuntos
Bacteroidetes/classificação , Florestas , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vietnã , Vitamina K 2/análogos & derivados , Vitamina K 2/química
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