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1.
Theriogenology ; 144: 164-173, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31972460

RESUMO

Interspecies hybridization exists widely in nature and plays an important role in animal evolution and adaptation. It is commonly recognized that male offspring of interspecies hybrid are often sterile, which presents a crucial way of reproductive isolation. Currently, the mechanisms underlying interspecies hybrid male sterility are not well understood. Cattle-yak, progeny of yak (Bos grunniens) and cattle (Bos taurus) cross, is a unique animal model for investigating hybrid male sterility. Because histone modifications are vital for spermatogenesis, herein, we examined expressions of histone methyltransferases (HMTs) and distributions of histone methylations in the yak and cattle-yak testis. Histological examination of seminiferous tubules revealed that gonocytes and spermatocytes were established normally, however, spermatogenesis was arrested at the meiosis phase began at 10 months after birth in the hybrids. SUV420H1 was the only HMT examined showing a significant enrichment in cattle-yak testes at 3 months. Relative expressions of MLL5, SETDB1 and SUV420H1 were increased while SETDB2 and EZH2 were decreased in cattle-yak testes at 10 months. Relative concentrations of MLL5 and SUV420H1 were again increased while EHMT2 and PRDM9 expressions were decreased at 24 months. Immunofluorescent detection of selected histone methylations in cross-sections of testicular tissues or meiotic chromosomes demonstrated that depletion of H3K4me3 and significant enrichment of H3K27me3 and H4K20me3 were observed in Sertoli cells of cattle-yak. Levels and localizations of H3K4me3, H3K9me1, H3K9me3 and H4K20me3 were strikingly different in meiotic chromosomes of cattle-yak spermatocytes. These results highlighted the potential roles of histone methylations in spermatogenic failure and hybrid male sterility.

2.
Genes (Basel) ; 11(1)2020 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-31940753

RESUMO

Spermatogenesis is a complex cellular-differentiation process that relies on the precise regulation of gene expression in spermatogonia, meiotic, and postmeiotic germ cells. The Ring 1 and YY1 binding protein (Rybp) is a member of the mammalian polycomb-group (PcG) protein family that plays multifunctional roles in development. Previous findings indicate that Rybp may function as an important regulator of meiosis. However, its expression in the testes and function in spermatogenesis have not been examined. In this study, we investigated Rybp expression in postnatal mouse testes using qRT-PCR and immunohistochemistry. We also examined the function of Rybp in spermatogenesis by using a conditional-knockout approach. Results showed that the relative expression of Rybp mRNA was significantly upregulated in the testes of postnatal day (PD) 6 mice. Immunofluorescent staining revealed that Rybp was enriched in the spermatocytes. Surprisingly, a conditional deletion of Rybp in fetal germ cells did not affect the fertility or normal development of spermatogenic cells. Further analysis revealed that Rybp deletion resulted in a decreased expression of meiosis-related genes, but that meiosis progression was normal. Together, these findings suggest that Rybp expression was enriched in spermatocytes, but that it was not required for spermatogenesis.

3.
Exp Appl Acarol ; 75(1): 69-84, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29611070

RESUMO

Isaria cateniannulata and Euseius nicholsi are two important biological control agents currently being used in many areas of China to control a variety of pests. In order to determine the possibility of a concomitant application with the two agents in a biocontrol program involving the two-spotted spider mite, Tetranychus urticae, we quantified the pathogenicity of a strain of I. cateniannulata (08XS-1) against females of both T. urticae and E. nicholsi. We observed the infection process using scanning electron microscopy and fluorescence microscopy to distinguish differences in fungal performance. The female mites were infected by I. cateniannulata at 2 × 107 conidia/ml. The mortality of T. urticae was 100% when treated with submerged conidia and 92% when treated with aerial conidia (spray), and that of E. nicholsi was 4.2 and 6.7%, correspondingly. Following infection with aerial or submerged conidia, mated E. nicholsi females displayed no significant differences between treatments and control, indicating the fungus had no obvious effect on their vitality and fertility. This demonstrates that I. cateniannulata is safe to E. nicholsi when used to control T. urticae. The two types of propagules of I. cateniannulata are readily produced by common culture, and the submerged conidia, because of their substantially higher mortality, are preferable to the aerial conidia. Our results indicate that I. cateniannulata and E. nicholsi are viable candidates to be concomitantly applied in the biocontrol programs of T. urticae.


Assuntos
Cordyceps/fisiologia , Cadeia Alimentar , Ácaros/microbiologia , Controle Biológico de Vetores , Controle de Ácaros e Carrapatos , Animais , Feminino , Especificidade da Espécie , Esporos Fúngicos , Tetranychidae/microbiologia
4.
PLoS One ; 12(5): e0177862, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28542422

RESUMO

Abnormal angiogenesis plays an important role in impaired wound healing and development of chronic wounds in diabetes mellitus. Angelica dahurica radix is a common traditional Chinese medicine with wide spectrum medicinal effects. In this study, we analyzed the potential roles of Angelica dahurica ethanolic extract (ADEE) in correcting impaired angiogenesis and delayed wound healing in diabetes by using streptozotocin-induced diabetic rats. ADEE treatment accelerated diabetic wound healing through inducing angiogenesis and granulation tissue formation. The angiogenic property of ADEE was subsequently verified ex vivo using aortic ring assays. Furthermore, we investigated the in vitro angiogenic activity of ADEE and its underlying mechanisms using human umbilical vein endothelial cells. ADEE treatment induced HUVECs proliferation, migration, and tube formation, which are typical phenomena of angiogenesis, in dose-dependent manners. These effects were associated with activation of angiogenic signal modulators, including extracellular signal-regulated kinase 1/2 (ERK1/2), Akt, endothelial nitric oxide synthase (eNOS) as well as increased NO production, and independent of affecting VEGF expression. ADEE-induced angiogenic events were inhibited by the MEK inhibitor PD98059, the PI3K inhibitor Wortmannin, and the eNOS inhibitor L-NAME. Our findings highlight an angiogenic role of ADEE and its ability to protect against impaired wound healing, which may be developed as a promising therapy for impaired angiogenesis and delayed wound healing in diabetes.


Assuntos
Angelica/metabolismo , Diabetes Mellitus Experimental/patologia , Neovascularização Fisiológica/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Cicatrização/efeitos dos fármacos , Androstadienos/farmacologia , Animais , Aorta/metabolismo , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Ativação Enzimática/efeitos dos fármacos , MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Flavonoides/farmacologia , Células Endoteliais da Veia Umbilical Humana , Humanos , Medicina Tradicional Chinesa , NG-Nitroarginina Metil Éster/farmacologia , Óxido Nítrico/biossíntese , Óxido Nítrico Sintase Tipo III/antagonistas & inibidores , Óxido Nítrico Sintase Tipo III/metabolismo , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Estreptozocina , Wortmanina
5.
Ying Yong Sheng Tai Xue Bao ; 28(5): 1633-1641, 2017 May 18.
Artigo em Chinês | MEDLINE | ID: mdl-29745202

RESUMO

Cadmium (Cd) is a common pollutant not required for life activities, which can have extremely strong toxicity to organisms and affect the growth of crops at a low concentration in soil. To investigate the molecular mechanism of soybean root responding to Cd stress, 7-day old soybean seedlings were stressed by Cd (75 Μmol·L-1) for 0 , 4 , 8, 12 and 48 h. Comparative transcriptome analysis showed 244, 1545, 442 and 1401 of genes responded to the four Cd treatments, respectively, and total 2670 differential expression genes were obtained. GO analysis classified these genes into 56 functional categories and COG analysis classified them into 25 functional categories. KEGG analysis showed that many genes involved in the phenylalanine metabolism, ubiquinone and other terpenoid-quinone biosynthesis and cysteine and methionine metabolism and so on. Further we found that expression of three isoflavones 2'-hydroxylase genes, two isoflavonereductase genes and a chalcone synthase gene were evidently up-regulated in all Cd treatments. The results of RT-PCR analysis of four DEGs were consistent with those of RNA-Seq data, further confirming the reliability of RNA-Seq results.


Assuntos
Cádmio/toxicidade , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Soja , Transcriptoma , Perfilação da Expressão Gênica , Metais Pesados , Reprodutibilidade dos Testes , Soja/metabolismo
6.
J Ethnopharmacol ; 193: 333-344, 2016 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-27558948

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Triptolide is a most important active ingredient extracted from traditional Chinese medicine Tripterygium, which has been widely used to treat glomerulonephritis as well as immune-mediated disorders, likely for its immunosuppressive, anti-proliferative and anti-inflammatory effects. AIM OF THE STUDY: In this study, we have investigated the potential protective effects of triptolide against diabetic cardiomyopathy (DCM) by regulating immune system, attenuating inflammatory response, thus resulting in decreased cardiac fibrosis and improved left ventricle function. MATERIALS AND METHODS: Sprague-Dawley rats were randomly divided into 5 groups: normal group, diabetic group and diabetic rats treated with triptolide (50, 100, or 200µg/kg/day resp) for 8 weeks. Cardiac function was performed by echocardiography and histopathology of the hearts was examined with HE, Masson staining and scanning electron microscopy. Immune regulation mediator, macrophage infiltration, inflammatory response and cardiac fibrosis related cytokines were measured by RT-PCR, Western blot and Immunohistochemistry staining. RESULTS: In the diabetic group, the expressions of TLR4 and NF-κB p65 were both up-regulated, which was associated with increased pro-inflammatory cytokines, coupled with cardiac fibrosis and impaired left ventricular function. Interestingly, pathological structure and function of left ventricle were both significantly improved in the triptolide treated groups. Furthermore, the immune mediator TLR4, downstream activator NF-κB p65, macrophage infiltration (CD68+), pro-inflammatory cytokines (TNF-α, IL-1ß), cell adhesion molecule (VCAM-1) and chemokine (MCP-1) were significantly suppressed when treated with medium and high dosage triptolide compared with the diabetic group. Moreover, cardiac fibrosis pathway including α-SMA, TGF-ß1, vimentin and collagen accumulations were observed significantly decreased in the triptolide treated groups. CONCLUSIONS: Our data demonstrated that the protective effects of triptolide against DCM might attribute to inhibition of TLR4-induced NF-κB/IL-1ß immune pathway, suppression of NF-κB/TNF-α/VCAM-1 inflammatory pathway and down-regulation of TGF-ß1/α-SMA/Vimentin fibrosis pathway.


Assuntos
Doenças Autoimunes/prevenção & controle , Cardiomiopatias/prevenção & controle , Cardiomiopatias Diabéticas/complicações , Diterpenos/farmacologia , Inflamação/prevenção & controle , Fenantrenos/farmacologia , Receptor 4 Toll-Like/fisiologia , Animais , Doenças Autoimunes/complicações , Glicemia/metabolismo , Cardiomiopatias/complicações , Compostos de Epóxi/farmacologia , Fibrose , Testes de Função Cardíaca/efeitos dos fármacos , Imunidade Inata/efeitos dos fármacos , Inflamação/complicações , Masculino , Ratos , Ratos Sprague-Dawley
7.
J Diabetes Res ; 2016: 5639129, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27057551

RESUMO

Impaired wound healing in diabetic patients is a serious complication that often leads to amputation or even death with limited effective treatments. Tuo-Li-Xiao-Du-San (TLXDS), a traditional Chinese medicine formula for refractory wounds, has been prescribed for nearly 400 years in China and shows good efficacy in promoting healing. In this study, we explored the effect of TLXDS on healing of diabetic wounds and investigated underlying mechanisms. Four weeks after intravenous injection of streptozotocin, two full-thickness excisional wounds were created with a 10 mm diameter sterile biopsy punch on the back of rats. The ethanol extract of TLXDS was given once daily by oral gavage. Wound area, histological change, inflammation, angiogenesis, and collagen synthesis were evaluated. TLXDS treatment significantly accelerated healing of diabetic rats and improved the healing quality. These effects were associated with reduced neutrophil infiltration and macrophage accumulation, enhanced angiogenesis, and increased collagen deposition. This study shows that TLXDS improves diabetes-impaired wound healing.


Assuntos
Indutores da Angiogênese/farmacologia , Anti-Inflamatórios/farmacologia , Complicações do Diabetes/tratamento farmacológico , Diabetes Mellitus Experimental/complicações , Medicamentos de Ervas Chinesas/farmacologia , Neovascularização Fisiológica/efeitos dos fármacos , Úlcera Cutânea/tratamento farmacológico , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Animais , Colágeno/biossíntese , Citocinas/metabolismo , Complicações do Diabetes/etiologia , Complicações do Diabetes/metabolismo , Complicações do Diabetes/patologia , Mediadores da Inflamação/metabolismo , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Masculino , Infiltração de Neutrófilos/efeitos dos fármacos , Ratos Sprague-Dawley , Pele/irrigação sanguínea , Pele/metabolismo , Pele/patologia , Úlcera Cutânea/etiologia , Úlcera Cutânea/metabolismo , Úlcera Cutânea/patologia , Fatores de Tempo
8.
Artigo em Inglês | MEDLINE | ID: mdl-26834629

RESUMO

Despite the involvement of subcortical brain structures in the pathogenesis of chronic pain and persistent pain as the defining symptom of knee osteoarthritis (KOA), little attention has been paid to the morphometric measurements of these subcortical nuclei in patients with KOA. The purpose of this study is to explore the potential morphological abnormalities of subcortical brain structures in patients with KOA as compared to the healthy control subjects by using high-resolution MRI. Structural MR data were acquired from 26 patients with KOA and 31 demographically similar healthy individuals. The MR data were analyzed by using FMRIB's integrated registration and segmentation tool. Both volumetric analysis and surface-based shape analysis were performed to characterize the subcortical morphology. The normalized volumes of bilateral caudate nucleus were significantly smaller in the KOA group than in the control group (P = 0.004). There was also a trend toward smaller volume of the hippocampus in KOA as compared to the control group (P = 0.027). Detailed surface analyses further localized these differences with a greater involvement of the left hemisphere (P < 0.05, corrected) for the caudate nucleus. Hemispheric asymmetry (right larger than left) of the caudate nucleus was found in both KOA and control groups. Besides, no significant correlation was found between the structural data and pain intensities. Our results indicated that patients with KOA had statistically significant smaller normalized volumes of bilateral caudate nucleus and a trend toward smaller volume of the hippocampus as compared to the control subjects. Further investigations are necessary to characterize the role of caudate nucleus in the course of chronicity of pain associated with KOA.

9.
J Diabetes Res ; 2015: 390428, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26347890

RESUMO

Tripterygium glycosides tablet (TGT) is a Chinese traditional medicine that has been shown to protect podocytes from injury and reduce the proteinuria. The aim of this study was to assess the effect of TGT on renal tubulointerstitial fibrosis and its potential mechanism in high-fat diet fed and STZ-induced diabetic rats. Rats were randomly divided into normal control rats (NC group), diabetic rats without drug treatment (DM group), and diabetic rats treated with TGT (1, 3, or 6 mg/kg/day, respectively) for 8 weeks. The results showed that 24 h proteinuria and urinary N-acetyl-glucosaminidase (NAG) in diabetic rats were decreased by TGT treatment without affecting blood glucose. Masson's trichrome stains showed that apparent renal tubulointerstitial fibrosis was found in DM group, which was ameliorated by TGT treatment. The expression of α-SMA was significantly decreased, accompanied by increased expression of E-cadherin in TGT-treated rats, but not in untreated DM rats. Further studies showed that TGT administration markedly reduced expression of TLR4, NF-κB, IL-1ß, and MCP-1 in TGT-treated diabetic rats. These results showed that TGT could ameliorate renal tubulointerstitial fibrosis, the mechanism which may be at least partly associated with the amelioration of EMT through suppression of the TLR4/NF-κB pathway.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Glicosídeos/farmacologia , NF-kappa B/metabolismo , Receptor 4 Toll-Like/metabolismo , Tripterygium/química , Acetilglucosaminidase/urina , Actinas/metabolismo , Animais , Glicemia/análise , Caderinas/metabolismo , Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2/sangue , Dieta Hiperlipídica , Relação Dose-Resposta a Droga , Transição Epitelial-Mesenquimal , Fibrose/metabolismo , Inflamação , Rim/metabolismo , Rim/patologia , Túbulos Renais/patologia , Masculino , Músculo Liso/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Estreptozocina/química , Tricomas/química
10.
J Zhejiang Univ Sci B ; 16(8): 672-9, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26238542

RESUMO

Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NaP) buffer solution (pH 5.0), 22 mmol/L 3,3',5,5'-tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H2O2), and 0.5% Tween-20 or 0.3% cetyltrimethyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1-2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (r=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.


Assuntos
Equipamentos Descartáveis , Análise de Alimentos/instrumentação , Contaminação de Alimentos/análise , Lactoperoxidase/análise , Leite/química , Fitas Reagentes , Animais , Cromatografia em Papel/instrumentação , Compostos Cromogênicos/química , Desenho de Equipamento , Análise de Falha de Equipamento , Pasteurização , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
11.
Exp Ther Med ; 10(1): 187-192, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26170933

RESUMO

The pathogenesis of atherosclerosis is multifactorial. The proliferation and migration of vascular smooth muscle cells (VSMCs) are significant in the genesis and development of atherosclerosis plaques, and the degradation of VSMCs plays a crucial role in the process. Mimecan is a member of the Keratan sulfate family of proteoglycans, which are leucine-rich proteoglycans. It has been demonstrated that mimecan is associated with arteriogenesis and atherosclerosis. In the present study, the effect of mimecan on the characteristics of cultured human aortic smooth muscle cells (HASMCs) was investigated. In vitro, human mimecan was stably overexpressed in HASMCs using a lentiviral system. It was observed that the proliferation rate of HASMCs transduced with mimecan was lower compared with that of control cells; overexpression of mimecan induced HASMC apoptosis. To determine the effect of mimecan on HASMC migration, a Transwell cell culture chamber and sterile cloning cylinder assays were used, and it was noted that mimecan enhanced the migration of HASMCs horizontally and vertically. These data indicated that mimecan may be involved in the pathogenesis of atherosclerosis by regulating the proliferation, apoptosis and migration of VSMCs.

12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 35(10): 2867-71, 2015 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-26904834

RESUMO

Castanea mollissima Blume has potential as an non-wood forest trees that have been cultivated for thousands of years in China. In order to elucidate the trace elements of chestnut ovary, the major trace elements of self- and cross-pollination chestnut ovary were determined by inductively coupled plasma mass spectrometry. The results showed that self- and cross-pollination 5-50 d, six trace elements trends showed fluctuations. After cross-pollination 20 d, the content of Ca was up to 6.50 mg x g(-1), while the self-pollination 10 d, the content of Ca reached up to 7.77 mg x g(-1). After cross- and self-pollination pollination 30 d, the content of Mg were highest, 4.19 and 4.69 mg x g(-1), respectively. After cross-pollination 5 d, the content of Zn reached the highest, 0.038 7 mg x g(-1), while self-pollination 10d the content of Zn was 0.039 9 mg x g(-1). After self- and cross-pollination 35 d, the content of Fe were 0.022, 0.019 mg x g(-1), respectively. After cross- and self-pollination 20 d, the content of Cu were 0.056, 0.045 mg x g(-1), respectively. After self-pollination 40d, the content of Mn reaching the highest was 1.204 mg x g(-1), while cross-pollination 30 d, the content of Mn reached its maximum 0.845 mg x g(-1). The results can provide a reference for spraying fertilizer on the ovary development, thereby improving chestnut production.


Assuntos
Polinização , Oligoelementos , China , Feminino , Humanos , Ovário , Espectrofotometria Atômica
13.
Zhongguo Zhong Yao Za Zhi ; 39(4): 733-7, 2014 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-25204156

RESUMO

OBJECTIVE: To investigate the effect of Zige lyophilized powder for injection in improving the acute cerebral microcirculation disturbance in rats. METHOD: Window craniotomy was performed for rats after the drug administration for 14 days. The experimental microcirculation disturbance model was duplicated with high molecule dextran. After the drug administration, the micro-vein diameters of cerebral pla mater of various groups were observed and recorded under the biological microscope. The blood flow volume was monitored by laser Doppler flow-meter. HCT was measured by the electric resistance method. The hemorheological indexes were detected by the auto-hemorheological instrument. RESULT: Zige lyophilized powder for injection (16.40, 32.70, 65.40 mg x kg(-1)) could significantly expand the micro-vein diameter of cerebral pla mater, improve the downward trend of the blood flow volume, and reduce the various hemorheological indexes. CONCLUSION: Zige lyophilized powder for injection shows the effect in improving the cerebral microcirculation.


Assuntos
Isquemia Encefálica/tratamento farmacológico , Circulação Cerebrovascular/efeitos dos fármacos , Medicamentos de Ervas Chinesas/administração & dosagem , Animais , Encéfalo/irrigação sanguínea , Encéfalo/efeitos dos fármacos , Isquemia Encefálica/fisiopatologia , Humanos , Masculino , Pós/administração & dosagem , Ratos , Ratos Sprague-Dawley
14.
Arch Gynecol Obstet ; 290(6): 1179-86, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25037597

RESUMO

PURPOSE: This study investigated proteins differentially expressed in the ovaries of menopausal women in comparison to childbearing women. METHODS: Differential protein expression was screened by difference gel electrophoresis and 2-D SDS-PAGE. Four differentially expressed proteins were excised manually, identified by mass spectrometry and confirmed by immunoblot and immunohistochemistry. RESULTS: The four proteins were identified as serum amyloid P, heat shock protein 27, Glyoxalase I and Ubiquitin carboxy-terminal hydrolase. Serum amyloid P expression was significantly up-regulated in the ovaries of menopausal women by immunoblot analysis (p < 0.05), Glyoxalase I and Ubiquitin carboxy-terminal hydrolase displayed an altered expression pattern, with higher expression in the atretic follicles of menopausal women. Weak Glyoxalase I and Ubiquitin carboxy-terminal hydrolase were observed in the granulosa and theca cells of the follicles of childbearing women. Heat shock protein 27 and serum amyloid P were clearly observed in the atretic follicles of menopausal women, while their expression was restricted to the theca cells and cytoplasm of primordial follicles in the ovaries of childbearing women. All four proteins were predominantly expressed in the atretic follicles of menopausal women. CONCLUSIONS: These data suggest that the identified proteins may play a role in the regulation of follicle atresia in menopausal women, although their functions and mechanism warrant further investigation.


Assuntos
Proteínas de Choque Térmico HSP27/metabolismo , Lactoilglutationa Liase/metabolismo , Menopausa/fisiologia , Ovário/metabolismo , Componente Amiloide P Sérico/metabolismo , Ubiquitina Tiolesterase/metabolismo , Adulto , Feminino , Corantes Fluorescentes , Atresia Folicular , Perfilação da Expressão Gênica/instrumentação , Perfilação da Expressão Gênica/métodos , Humanos , Immunoblotting , Imuno-Histoquímica , Espectrometria de Massas , Folículo Ovariano/metabolismo , Células Tecais/metabolismo , Eletroforese em Gel Diferencial Bidimensional
15.
Cell Immunol ; 289(1-2): 155-61, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24838092

RESUMO

The chemokine CCL21 is a potent chemoattractant for T cells and dendritic cells. IL-15 elicits powerful antitumor immune responses through the stimulation of natural killer cells. We constructed a CCL21/IL-15-expressing adenovirus (Ad-CCL21-IL-15) and evaluated its antitumor effects in vitro and in vivo. We found that the intratumoral injection of Ad-CCL21-IL-15 into murine colon carcinomas significantly inhibited tumor growth. Splenocytes from mice treated with Ad-CCL21-IL-15 developed tumor-specific cytotoxic T cells and were protected from subsequent challenges with tumor cells. This study indicates that providing cancer therapy by combining CCL21 and IL-15 can induce antitumor immune responses and is an effective strategy for cancer immunotherapy.


Assuntos
Quimiocina CCL21/genética , Neoplasias do Colo/terapia , Terapia Genética , Interleucina-15/genética , Linfócitos T Citotóxicos/imunologia , Células 3T3 , Adenoviridae , Animais , Vacinas Anticâncer/administração & dosagem , Vacinas Anticâncer/imunologia , Vacinas Anticâncer/uso terapêutico , Linhagem Celular Tumoral , Movimento Celular/genética , Movimento Celular/imunologia , Células Dendríticas/imunologia , Técnicas de Transferência de Genes , Humanos , Imunoterapia , Camundongos
16.
Chin J Nat Med ; 12(3): 204-12, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24702807

RESUMO

AIM: To explore the therapeutic effects of Morinda officinalis capsules (MOP) on osteoporosis in ovariectomized rats. METHODS: Six-month-old female Sprague-Dawley rats were induced for postmenopausal osteoporosis (PMOP) by bilateral ovariectomy and divided into seven groups as follows: sham-operated group, ovariectomized (OVX) control group, OVX treated with xianlinggubao (XLGB) (270 mg·kg⁻¹·d⁻¹), OVX treated with alendronate sodium (ALN) (3 mg·kg⁻¹·d⁻¹), and OVX treated with Morinda officinalis capsule (MOP) of graded doses (90, 270 and 810 mg·kg⁻¹·d⁻¹) groups. Oral treatments were administered daily on the 4(th) week after ovariectomy and lasted for 12 weeks. The bone mineral density was evaluated by dual-energy X-ray absorptiometry. The tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (AKP), and osteocalcin (OC) levels in the serum and plasma were determined by standard colorimetric and enzyme immunoassays methods. Bone biomechanical properties and morphological parameters were analyzed by three-point bending test and histomorphometry respectively. RESULTS: Morinda officinalis capsules at all doses were able to significantly prevent the OVX-induced loss of bone mass due to diminishing serum AKP and TRAP levels while elevating OC level in the plasma. Morinda officinalis capsules also enhanced the bone strength and prevented the deterioration of trabecular microarchitecture. CONCLUSION: Morinda officinalis capsules possess potent anti-osteoporotic activity in OVX rats which could be an effective treatment for postmenopausal osteoporosis.


Assuntos
Conservadores da Densidade Óssea/uso terapêutico , Densidade Óssea/efeitos dos fármacos , Medicamentos de Ervas Chinesas/uso terapêutico , Morinda , Osteocalcina/sangue , Osteoporose Pós-Menopausa/prevenção & controle , Fitoterapia , Fosfatase Ácida/sangue , Fosfatase Alcalina/sangue , Animais , Conservadores da Densidade Óssea/farmacologia , Cápsulas , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Humanos , Isoenzimas/sangue , Osteoporose Pós-Menopausa/sangue , Osteoporose Pós-Menopausa/metabolismo , Ovariectomia , Ratos , Ratos Sprague-Dawley , Fosfatase Ácida Resistente a Tartarato
17.
Huan Jing Ke Xue ; 34(1): 188-93, 2013 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-23487937

RESUMO

La-N co-doped TiO2 photocatalyst was synthesized by a sol-gel method, which used tetrabutyl titanate (TBOT) as the Ti resource, lanthanum nitrate as the La resource and urea as the N resource. The performance in the photocatalytic oxidation degradation of malachite green was investigated. The results showed that the reaction could be reasonably represented by first order kinetics, and a kinetic model was established; the reaction order of voltage (0.935 8) was higher than that of pH (0.6119) and that of initial concentration (0.335), indicating that voltage has the greatest impact on the reaction. The model data fitted well with experimental data (R2 0.910-0.9961), which indicates that the kinetic model can well describe the process of photocatalytic degradation of malachite green.


Assuntos
Processos Fotoquímicos , Corantes de Rosanilina/isolamento & purificação , Titânio/química , Poluentes Químicos da Água/isolamento & purificação , Catálise , Eletroquímica , Cinética , Corantes de Rosanilina/química , Poluentes Químicos da Água/química
18.
Clin Endocrinol (Oxf) ; 79(2): 267-74, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23170961

RESUMO

OBJECTIVE: Associations between IL2RA and various autoimmune diseases have been reported in Caucasians. We investigated whether genetic polymorphisms at the IL2RA locus were associated with Graves' disease (GD) in the Chinese Han population. DESIGN: We performed a genome-wide association study (GWAS) in 1 536 GD patients and 1 516 controls. The 1000 Genomes Project data were adopted as references for imputation analysis. After forward and conditional logistic regressions, we found that rs11256313 was the major risk variant in the CD25/IL2RA region. Thus, we further genotyped rs11256313 in a replication cohort with 3 694 GD patients and 3 510 controls using ABI 7900HT TaqMan Real-Time PCR System. RESULTS: Nine single nucleotide polymorphisms (SNPs) in the IL2RA block were nominally associated with GD in our GWAS (0·01 < P < 0·05). After imputation analysis, 13 imputed SNPs in the IL2RA block were weakly associated with GD (P ≤ 0·05). Logistic regression analysis suggested that the imputed rs11256313 could represent the IL2RA block (P = 0·003). However, we failed to replicate the association of rs11256313 in a larger cohort (P = 0·145). A subphenotype analysis of rs11256313 on thyroid hormone receptor antibody (TRAb) and gender showed that there was no association in any of the subphenotype groups (P > 0·05). CONCLUSIONS: The results suggested that common genetic polymorphisms at IL2RA do not exert a significant genetic effect on the development of GD in the Chinese Han population. Previously reported associations between CD25/IL2RA and autoimmune diseases including GD in Caucasians again imply that heterogeneity exists in different ethnic populations.


Assuntos
Doença de Graves/genética , Subunidade alfa de Receptor de Interleucina-2/genética , Grupo com Ancestrais do Continente Asiático/genética , China/epidemiologia , Grupos Étnicos/genética , Estudo de Associação Genômica Ampla , Genótipo , Doença de Graves/epidemiologia , Humanos , Polimorfismo de Nucleotídeo Único
19.
Huan Jing Ke Xue ; 33(4): 1267-71, 2012 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-22720576

RESUMO

Malachite green oxidation degradation was kinetically investigated in a photoelectrocatalytic reactor, using Pr-N co-doped TiO2 photocatalyst as the electrode which was prepared by a sol-gel method. The result shows that the initial concentration, pH, voltage and temperature had a significant impact on the oxidation rate. The kinetic equation for malachite green oxidation under the conditions of 10-30 mg x L(-1) of initial concentration, 3-8 of pH, 1-5 V of voltage, 298-338 K of temperature could be described using the first order kinetics, which was fitted well with the experimental data. The lower activation energy of 11.99 kJ x mol(-1) shows the reaction can be initiated easily; The reaction order of pH (1.634 7) is higher than that of voltage (0.850 2) and initial concentration (0.123 8), which indicates that the oxidation rate can be controlled efficiently through adjusted pH.


Assuntos
Corantes de Rosanilina/isolamento & purificação , Titânio/química , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/química , Catálise , Luz , Nitrogênio/química , Processos Fotoquímicos , Praseodímio/química
20.
Mol Cell Biochem ; 360(1-2): 321-8, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21938403

RESUMO

Mimecan, a secretary protein that is expressed in mouse and human pituitary corticotroph cells, is up-regulated by glucocorticoids (GC) in the corticotroph cells via classical glucocorticoid receptor (GR) pathways. In this study, we further explore the GC mechanism for mimecan expression in these cells. Five putative GR response elements (GREs) were identified in ~2 kb of the mimecan promoter by programme analysis. An EMSA assay further indicated that these putative GREs were bound by the GR. Moreover, three proximal GREs are conserved between species. Although luciferase assays showed that the -1474/+43 region of the mimecan promoter achieved the highest expression of mimecan, the -803/+43 mimecan promoter region was sufficient for the GC-mediated expression of mimecan. The mutations of three conserved GREs located in the -1474/+43 mimecan promoter region did not affect mimecan transcription, which suggests that the effects of GC on mimecan are independent of the GREs in the promoter. In addition, cycloheximide, a protein synthesis inhibitor, blocked GC-induced mimecan expression in AtT-20 cells. Taken together, these results suggest that, although there are 3-5 GREs in the mimecan promoter, GC may regulate mimecan transcription indirectly through the synthesis of intermediate proteins and not through the GREs in pituitary corticotroph cells.


Assuntos
Corticotrofos/metabolismo , Glucocorticoides/fisiologia , Peptídeos e Proteínas de Sinalização Intercelular/genética , Transcrição Genética , Animais , Sequência de Bases , Linhagem Celular , Sequência Conservada , Regulação da Expressão Gênica , Glucocorticoides/farmacologia , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Camundongos , Dados de Sequência Molecular , Receptores de Glucocorticoides/metabolismo , Elementos de Resposta
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