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1.
Artigo em Inglês | MEDLINE | ID: mdl-32167894

RESUMO

In this paper, we proposed a new end-to-end model, termed as dual-discriminator conditional generative adversarial network (DDcGAN), for fusing infrared and visible images of different resolutions. Our method establishes an adversarial game between a generator and two discriminators. The generator aims to generate a real-like fused image based on a specifically designed content loss to fool the two discriminators, while the two discriminators aim to distinguish the structure differences between the fused image and two source images, respectively, in addition to the content loss. Consequently, the fused image is forced to simultaneously keep the thermal radiation in the infrared image and the texture details in the visible image. Moreover, to fuse source images of different resolutions, e.g., a low-resolution infrared image and a high-resolution visible image, our DDcGAN constrains the downsampled fused image to have similar property with the infrared image. This can avoid causing thermal radiation information blurring or visible texture detail loss, which typically happens in traditional methods. In addition, we also apply our DDcGAN to fusing multi-modality medical images of different resolutions, e.g., a low-resolution positron emission tomography image and a high-resolution magnetic resonance image. The qualitative and quantitative experiments on publicly available datasets demonstrate the superiority of our DDcGAN over the state-of-the-art, in terms of both visual effect and quantitative metrics.

2.
Ying Yong Sheng Tai Xue Bao ; 31(1): 25-34, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31957377

RESUMO

To examine the effects of management measures on carbon and nitrogen contents, as well as their distribution and structural characteristics of different soil fractions in Moso bamboo plantations, we compared three types of the bamboo forests (undisturbed, extensively managed, and intensively managed) and the control secondary broadleaved evergreen forest using the methods of physical fractionation, chemical and biological analysis and Fourier-transform infrared spectroscopy (FTIR). The results showed that soil total organic carbon (TOC) and total nitrogen (TN) content, as well as free particulate organic carbon and nitrogen, soluble organic carbon and nitrogen (DOC, DON), and mineral-associated organic carbon and nitrogen in the undisturbed and extensively managed Moso bamboo plantations were significantly increased compared with that in the control. The distribution ratio of free particulate organic carbon and nitrogen in the undisturbed Moso bamboo plantation significantly increased, with mineral-associated organic carbon being the largest reservoir of soil organic carbon (67.6%). Intensive management resulted in the decrease of soil organic carbon, total nitrogen storage, and the contents of each component, but significantly increased DOC/TOC, the ratio of microbial biomass nitrogen to TN as well as the ratio of microbial biomass carbon to TOC (microbial quotient). Management measures significantly affected the chemical structure of SOC. Compared with the control, the relative intensities of phenolic and alcoholic-OH, aliphatic methyl and methylene, aromatic C=C, and carbonyl C=O absorption were higher in the SOC of undisturbed and extensively managed Moso bamboo plantations, and soil hydrophobicity was significantly increased. Results from correlation analysis showed that soil hydrophobicity and the content of aliphatic and aromatic groups were negatively correlated with microbial quotient and positively correlated with TOC and TN content. In conclusion, the increased inputs of organic matter residues (such as litter and roots) could contribute to the relative accumulation of chemical resistance compounds with reduced human disturbance, which significantly enhanced chemical stability of soil organic carbon. Soil clay minerals played a key role in protecting soil organic carbon through the formation of mineral-organic compounds, which facilitate the stability of soil carbon storage and the long-term preservation of soil carbon.


Assuntos
Carbono , Nitrogênio , China , Florestas , Humanos , Poaceae , Solo
3.
Artigo em Inglês | MEDLINE | ID: mdl-31976898

RESUMO

Audio information has been bypassed by most of current visual attention prediction studies. However, sound could have influence on visual attention and such influence has been widely investigated and proofed by many psychological studies. In this paper, we propose a novel multi-modal saliency (MMS) model for videos containing scenes with high audio-visual correspondence. In such scenes, humans tend to be attracted by the sound sources and it is also possible to localize the sound sources via cross-modal analysis. Specifically, we first detect the spatial and temporal saliency maps from the visual modality by using a novel free energy principle. Then we propose to detect the audio saliency map from both audio and visual modalities by localizing the moving-sounding objects using cross-modal kernel canonical correlation analysis, which is first of its kind in the literature. Finally we propose a new two-stage adaptive audiovisual saliency fusion method to integrate the spatial, temporal and audio saliency maps to our audio-visual saliency map. The proposed MMS model has captured the influence of audio, which is not considered in the latest deep learning based saliency models. To take advantages of both deep saliency modeling and audio-visual saliency modeling, we propose to combine deep saliency models and the MMS model via a later fusion, and we find that an average of 5% performance gain is obtained. Experimental results on audio-visual attention databases show that the introduced models incorporating audio cues have significant superiority over state-of-the-art image and video saliency models which utilize a single visual modality.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31879145

RESUMO

OBJECTIVES: Thoracic aortic dissection (TAD) has a high mortality rate. Intermittent hypoxia (IH) triggers both harmful and beneficial effects in numerous physiological systems. The effects of IH on TAD development were explored in a mouse model. METHODS: ß-Aminopropionitrile monofumarate (BAPN) was used to induce TAD in C57BL/6 mice. Three week old male mice were treated with 1 g/kg/day BAPN in drinking water for four weeks and simultaneously subjected to IH (n = 30) (21%-5% O2, 90 s/cycle, 10 h/day, IH + BAPN group) or normoxia (n = 30) (21% O2, 24 h/day, BAPN group). Human VSMCs (HUASMCs) exposed to IH (30 min, 5% O2)/re-oxygenation (30 min, 21% O2) cycles with a maximum of 60 min/cycle to detect the effect of IH on HIF-1α and LOX via HIF-1α-siRNA. RESULTS: It was found that BAPN administration significantly increased the lumen size and wall thickness of aortas compared with the normal group, but was significantly reversed by IH exposure. Additionally, IH exposure significantly increased the survival rate of BAPN induced TAD (70% vs. 40%). Furthermore, IH exposure reduced BAPN induced elastin breaks and apoptosis of vascular smooth muscle cells. IH exposure also reversed BAPN induced upregulation of inflammation and extracellular matrix (ECM) degradation. Real time polymerase chain reaction (RT-PCR) confirmed that IH inhibited inflammation and ECM degradation related genes interleukin (IL)-1ß, IL-6, cathepsin S (Cat S), and matrix metalloproteinase 9 (MMP-9), but upregulated the ECM synthesis related genes lysyl oxidase (LOX) and collagen type I alpha2 (Col1a2) compared with the BAPN group. In vitro results suggest that IH promotes the expression of LOX via HIF-1α. CONCLUSIONS: The results suggest that IH alleviates BAPN induced TAD in C57BL/6 mice.

5.
Anal Chem ; 91(24): 15866-15872, 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31756075

RESUMO

The combination of gold nanoclusters (AuNCs) and nanomaterial-based quencher creates an innovative method for sensors design. In this work, we report a fluorescent sensing platform for sensitive detection of butyrylcholinesterase (BChE). The fluorescence of AuNCs can be quenched by iron oxyhydroxide (FeOOH) nanomaterials. In the presence of BChE and acetylthiocholine (ATCh), nano-FeOOH can be effectively decomposed by the enzymatic hydrolysate (thiocholine), leading to the recovery of AuNCs fluorescence. The Au/FeOOH exhibits the highest fluorescence quenching efficiency compared with other transition metal oxyhydroxide-based sensing systems, e.g., Au/CoOOH and Au/NiOOH. The corresponding fluorescence recovery efficiency is also the best for Au/FeOOH. The large surface area of nanomaterials and thin nanostructure provide a favorable platform for the reaction of enzymatic hydrolysate and eventually improve the high sensitivity of the probe. A linear detection range for BChE is achieved within 5-100 ng mL-1 along with a detection limit of 4 ng mL-1. By taking advantage of the high sensitivity, the Au/FeOOH was successfully used for BChE quantification in 2 µL of finger blood.

6.
Angew Chem Int Ed Engl ; 58(51): 18394-18398, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31628706

RESUMO

An exceptionally strong solvation effect of dimethyl sulfoxide (DMSO) on I2 is identified by the largest shift observed so far of the I2 Raman peak with respect to I2 vapor and by elongated I-I bond lengths in first-principles molecular-dynamics simulations. This effect together with strong binding by an RuO2 surface to I2 is found to invert the direction of the reaction I- +I2 ⇌I3 - to the left-hand side. Inspired by this finding, we prepared a Li-O2 battery with the Li/DMSO+LiI/RuO2 structure. The synergic action of DMSO and RuO2 on I2 is found to suppress the shuttle effect of the redox mediator (RM) by anchoring I2 molecules, the oxidation product of the RM. Significantly enhanced stability is demonstrated over 100 cycles at charging voltage below 3.65 V.

7.
Mol Med Rep ; 20(5): 4226-4234, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31545471

RESUMO

Damage to the blood­brain barrier (BBB) resulting from systemic inflammation caused by surgical trauma is associated with cognitive dysfunction, and individuals with hyperlipidemia are more sensitive to such impairment. The present study was designed to ascertain whether dexmedetomidine (Dex) treatment could reduce the incidence of cognitive dysfunction following surgery in a hyperlipidemia model. Hyperlipidemia was induced in Sprague­Dawley rats (male, 6­7 months old) by consuming a high­fat diet, and rats were divided into three groups (n=10 each) and underwent: exploratory laparotomy to introduce surgical trauma (surgery group), laparotomy and Dex treatment (surgery+Dex group), or sham surgery (sham group). Learning, memory and exploration behavior were assessed using the Morris water maze. Concentrations of tumor necrosis factor (TNF)­α and interleukin (IL)­1ß, were determined by enzyme­linked immunosorbent assay. BBB permeability was assessed by Evans blue staining. Relative major facilitator superfamily domain­containing protein 2 (Mfsd2a) mRNA expression was determined by quantitative PCR. In the Morris water maze test, the time and distance ratio for the surgery group was significantly lower than those of the sham and surgery+Dex groups (P<0.05). In addition, the TNF­α concentrations in the sham and surgery+Dex groups were lower than that in the surgery group (P<0.05 on days 1 and 3). Evans Blue staining was increased in the surgery group on day 1 (P<0.01). Mfsd2a mRNA expression was higher in the sham and surgery+Dex groups compared with that noted in the surgery group (P<0.05). In conclusion, Dex treatment decreased the incidence of cognitive dysfunction following surgical trauma in a hyperlipidemia rat model. We demonstrated that Dex stabilized BBB integrity through increased Mfsd2a gene expression.


Assuntos
Barreira Hematoencefálica/metabolismo , Dieta Hiperlipídica , Hiperlipidemias/complicações , Proteínas de Membrana Transportadoras/metabolismo , /metabolismo , Transdução de Sinais , Animais , Dexmedetomidina/farmacologia , Modelos Animais de Doenças , Regulação da Expressão Gênica/efeitos dos fármacos , Hiperlipidemias/dietoterapia , Proteínas de Membrana Transportadoras/genética , Permeabilidade , RNA Mensageiro/genética , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos
8.
Math Biosci Eng ; 16(5): 5687-5696, 2019 06 19.
Artigo em Inglês | MEDLINE | ID: mdl-31499732

RESUMO

Background: The current standard approach to the treatment of patients with non-small-cell lung cancer (NSCLC) harboring epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI)-sensitizing mutations has been the treatment with a first-generation EGFR-TKIs. While, with resistance developed against first-generation EGFR-TKIs, second/third-generation TKIs have attracted all the attention, and replaced first-generation EGFR- TKIs upon disease progression due to the greater efficacy and more favorable tolerability. In the past few years, this strategy has been challenged by clinical evidence when next-generation EGFR-TKIs are used in patients with advanced NSCLC. Objective: In this study, we performed a meta- analysis to investigate the efficacy of next-generation TKIs comparison with first-generation TKIs in the treatment of NSCLC. Methods: The multiple databases including Pubmed, Embase, Cochrane library databases were adopted to search for the relevant studies, and full-text articles involving to comparison of next-generation TKIs and first-generation TKIs were reviewed. After rigorous reviewing on quality, the data was extracted from eligible randomized controlled trial (RCT). Meta-analysis Revman 5.3 software was used to analyze the combined pooled ORs with the corresponding 95% confidence interval using fixed- or random-effects models according to the heterogeneity. Results: A total of 5 randomized controlled trials were included in this analysis. The group of next-generation TKIs did achieved benefit in progression-free survival (PFS) (OR = 0.58, 95%CI = 0.45-0.75, P<0.0001), overall survival (OS) (OR = 0.76, 95%CI = 0.65-0.90, P = 0.001) as well with the objective response rate (ORR) (OR = 1.27, 95%CI = 1.01-1.61, P = 0.04), respectively. In the results of subgroup analysis of PFS with EGFR mutations, there is also significant differences with exon 19 deletion (OR = 0.56, 95%CI = 0.41-0.77, P = 0.0003) and exon 21 (L858R) mutation (OR = 0.60, 95%CI = 0.49-0.75, P<=0.00001). While, the treatment-related severe adverse event (SAE) between the next-generation TKIs and first-generation TKIs did not have statistical significance (OR = 1.48, 95%CI = 0.62-3.55, P = 0.38). Conclusion: The next-generation TKIs significantly improved efficacy outcomes in the treatment of EGFR mutation-positive advanced NSCLC compared with the first-generation TKIs, with a manageable safety profile. These results are potentially important for clinical decision making for these patients.

9.
Nat Commun ; 10(1): 3543, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31391469

RESUMO

As soluble catalysts, redox mediators can reduce the high charging overpotential of lithium-oxygen batteries by providing sufficient liquid-solid interface for lithium peroxide decomposition. However, the redox mediators usually introduce undesirable reactions. In particular, the so-called "shuttle effect" leads to the loss of both the redox mediators and electrical energy efficiency. In this study, an organic compound, triethylsulfonium iodide, is found to act bifunctionally as both a redox mediator and a solid electrolyte interphase-forming agent for lithium-oxygen batteries. During charging, the organic iodide exhibits comparable lithium peroxide-oxidizing capability with inorganic iodides. Meanwhile, it in situ generates an interfacial layer on lithium anode via reductive ethyl detaching and the subsequent oxidation. This layer prevents the lithium anode from reacting with the redox mediators and allows efficient lithium-ion transfer leading to dendrite-free lithium anode. Significantly improved cycling performance has been achieved by the bifunctional organic iodide redox mediator.

10.
J Cancer ; 10(10): 2299-2311, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31258733

RESUMO

Background: Lymphocytes were reported to play a significant part in host anticancer immune responses and influence tumour prognosis. Few studies have focused on the prognostic values of aspartate aminotransferase (AST) to lymphocyte ratio (ALRI), aspartate aminotransferase to platelet count ratio index (APRI) and systemic immune-inflammation index (SII) in hepatocellular carcinoma (HCC) treated with palliative treatments. Methods: Five hundred and ninety-eight HCC patients treated with palliative therapies were retrospectively analysed. We randomly assigned patients into the training cohort (429 patients) and the validation cohort I (169 patients). Receiver operating characteristic (ROC) curves were used to identify the best cut-off values for the ALRI, APRI and SII in the training cohort and the values were further validated in the validation cohort I. Correlations between ALRI and other clinicopathological factors were also analysed. A prognostic nomogram including ALRI was established. We validated the prognostic value of the ALRI, SII and APRI with two independent cohorts, the validation cohort II of 82 HCC patients treated with TACE and the validation cohort III of 150 HCC patients treated with curative resection. In the training cohort and all the validation cohorts, univariate analyses by the method of Kaplan-Meier and multivariate analysis by Cox proportional hazards regression model were carried out to identify the independent prognostic factors. Results: The threshold values of ALRI, APRI and SII were 86.3, 1.37 and 376.4 respectively identified by ROC curve analysis in the training cohort. Correlation analysis showed that ALRI>86.3 was greatly associated with higher rates of Child-Pugh B&C, portal vein tumor thrombosis (PVTT) and ascites (P < 0.05). Correspondingly, ALRI level of HCC patients with Child-Pugh B&C, PVTT and ascites was evidently higher than that of HCC patients with Child-Pugh A, without PVTT and without ascites (P < 0.001). In the training cohort and the validation cohort I, II, III, the OS of patients with ALRI >86.3 was obviously shorter than patients with ALRI ≤86.3 (P <0.001). We identified ALRI as an independent prognostic factor by univariate and multivariate analyses both in training Cohort (HR=1.481, P=0.004), validation cohort I (HR=1.511, P=0.032), validation cohort II (HR=3.166, P=0.005) and validation cohort III (HR=3.921, P=0.010). The SII was identified as an independent prognostic factor in training cohort (HR=1.356, P=0.020) and the validation cohort II (HR=2.678, P=0.002). The prognostic nomogram including ALRI was the best in predicting 3-month, 6-month, 1-year, 2-year survival And OS among TNM, ALRI, ALRI-TNM and nomogram. Conclusions: The ALRI was a novel independent prognostic index for the HCC patients treated with palliative treatments.

11.
Curr Med Sci ; 39(4): 546-550, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31346989

RESUMO

In this study, we used plasma factor V activity and parameters of the thrombin generation test to discuss their diagnostic and prognostic value for disseminated intravascular coagulation (DIC) in patients with hematological malignancies. A total of 164 patients who were diagnosed with hematological malignancies in the Department of Hematology, Union Hospital, between Apr. 2014 and Dec. 2014 were enrolled in this study. There were 131 patients in the study group and 33 patients in the control group in terms of the laboratory results for DIC. The patients in the study group were divided into a DIC subgroup (n=59) and a non-DIC subgroup (n=72) based on the International Society of Thrombosis and Hemostasis (ISTH) Integral System, and they were divided into four subgroups [score ≤3 (n=35), score=4 (n=37), score=5 (n=47), and score ≥6 (n=12)] according to ISTH scores. Using 28-day mortality as the endpoint, the patients in the study group were divided into a survival subgroup (n=111) and a non-survival subgroup (n=20). The results showed that the plasma factor V activity was significantly weaker, and lag time and time to peak were significantly shorter in the study group than in the control group (P<0.01). The factor V activity, peak and endogenous thrombin potential (ETP) were significantly decreased in the DIC subgroup as compared with those in the non-DIC subgroup (P<0.01). Among factor V activity, lag time, peak, ETP, and ttPeak, only the factor V activity was significantly decreased in the non-survival subgroup compared with the survival subgroup (P<0.01). With the increase in ISTH score, the ETP and peak decreased gradually. The binary logistic regression analysis revealed that PLT, D-dimer, factor V activity and ETP had linear relationship with DIC diagnosed by ISTH Integral System. Using DIC diagnosed by ISTH Integral System as the endpoint, the area under curve (AUC) of factor V activity was found to be similar to that of blood platelet count (PLT) and prothrombin time (PT). In conclusion, factor V activity, ETP and peak had diagnostic value for DIC in patients with hematological malignancies, and only factor V activity had limited prognostic value.


Assuntos
Coagulação Intravascular Disseminada/sangue , Fator V/metabolismo , Neoplasias Hematológicas/sangue , Prognóstico , Adulto , Testes de Coagulação Sanguínea , Coagulação Intravascular Disseminada/patologia , Feminino , Neoplasias Hematológicas/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Contagem de Plaquetas , Curva ROC , Trombina/metabolismo , Trombose/sangue , Trombose/genética , Trombose/patologia
12.
Analyst ; 144(14): 4175-4179, 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31237576

RESUMO

In this work, we report a facile, sensitive, selective, and reproducible DNA impedimetric sensor device. We demonstrate that, combined with exonuclease III, the easily prepared electrochemically reduced graphene oxide (rGO) could be a desirable platform to amplify signals in electrochemical impedance spectroscopy for ultrasensitive DNA detection. Guided by enzyme assisted target recycling, efficient interfacial tuning can be obtained, from the situation with high impedance caused by single-stranded DNA probes directly adsorbed onto rGO to the one with low impedance due to the continuous desorption of target-probe DNA hybrids and the consequent digestion of DNA probes. Just a few DNA targets can specifically trigger the enzymatic digestion of a large number of DNA probes. It is the excellent electrical conductivity of rGO that further enlarges the changes of electron transfer resistance after the removal of DNA probes. As a result of synergistically combining both enzymatic and electrical amplification, the enlarged changes of impedimetric signals can be measured to sensitively report DNA targets. The specificity has been guaranteed by the intrinsic recognition of hybrids through both rGO and exonuclease III. A limit of detection as low as 10 aM target DNA in the matrix of cell culture medium, as well as a wide linear range and good discrimination of mismatched sequences even at the one-base level, suggests its great application prospect in biosensing and biomedical analysis. It also has other advantages including easy operation, low cost, and convenient regeneration, with more competitive performance in developing impedimetric biosensors.


Assuntos
DNA/análise , Técnicas Biossensoriais/métodos , DNA/química , DNA/genética , Sondas de DNA/genética , Espectroscopia Dielétrica/métodos , Exodesoxirribonucleases/química , Grafite/química , Limite de Detecção , Técnicas de Amplificação de Ácido Nucleico/métodos , Hibridização de Ácido Nucleico
13.
Curr Med Sci ; 39(1): 94-98, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30868497

RESUMO

Using anesthetic gel may not sufficiently exclude pain perception during and after cystoscopy in male patients. To evaluate the analgesic efficacy and safety of intramuscular parecoxib (40 mg) for outpatient-based rigid cystoscopy, we performed a prospective, randomized and controlled study. Consecutive male patients requiring diagnostic cystoscopy in our hospital were divided into group A (1% tetracaine gel, n=50) and group B (parecoxib, n=51) at random. Patients received intramuscular injections of either 2 mL sterile saline in group A or 40 mg parecoxib in group B 30 min before the procedure. Tetracaine gel was injected into the urethra 3 min before the procedure in group A, with patients receiving plain lubricant gel in group B at the same time. Cystoscopy-associated pain levels were evaluated using the Visual Analog Score (VAS) during the procedure. Post-procedure urethral pain and complications were recorded and analyzed. The results showed that male patients experienced significantly less pain in group B than in group A (2.70±1.36 vs. 3.56±1.74, P=0.008). The percentage of patients with dysuria pain was not significantly different between the two groups. In addition, 24 h after cystoscopy, the patients with no previous experience of cystoscopy were more likely to declare urethral pain (59.2% vs. 33.3%, P=0.012, relative risk=1.78). No difference was observed in analgesic-related complications between the two groups. We conclude that intramuscular injection of 40 mg parecoxib may improve comfort for male patients undergoing rigid cystoscopy.


Assuntos
Inibidores de Ciclo-Oxigenase 2/administração & dosagem , Cistoscopia/efeitos adversos , Isoxazóis/administração & dosagem , Dor/tratamento farmacológico , Tetracaína/administração & dosagem , Instituições de Assistência Ambulatorial , Inibidores de Ciclo-Oxigenase 2/uso terapêutico , Cistoscopia/instrumentação , Método Duplo-Cego , Humanos , Injeções Intramusculares , Isoxazóis/uso terapêutico , Masculino , Pessoa de Meia-Idade , Dor/etiologia , Manejo da Dor , Medição da Dor , Estudos Prospectivos , Tetracaína/uso terapêutico , Resultado do Tratamento
14.
Cell Death Dis ; 10(3): 159, 2019 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-30770793

RESUMO

Notwithstanding the researches on biomarkers and targeted therapies in renal cell carcinomas (RCC) have made progress in the last decades, the application of the biomarkers and targeted therapy agents for RCC in clinic are restricted because of their limitation or side effects. Liver X receptors (LXRs) and the NLRP3 inflammasome have been the research hotspots in recent years. In our study, we integrated bioinformatics analysis, molecular biology experiments and biological function experiments to study the roles of LXRα and the NLRP3 inflammasome in RCC. The study demonstrated that the elevated LXRα expression is correlated with a poor prognosis in RCC. Furthermore, our study revealed the expression levels and roles of the NLRP3 inflammasome in RCC for the first time. This research demonstrated that LXRα could promote the metastasis of RCC cells by suppressing the expression of the NLRP3 inflammasome. In Brief, LXRα had the possibility to be a novel diagnostic and prognostic biomarker and therapeutic target in renal cell cancer and LXRα could regulate the metastasis of renal cell cancer via NLRP3 inflammamsome.

15.
J Zhejiang Univ Sci B ; 20(2): 180-192, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30666850

RESUMO

To understand the effects of Lactobacillus rhamnosus GG (ATCC 53103) on intestinal barrier function in pre-weaning piglets under normal conditions, twenty-four newborn littermate piglets were randomly divided into two groups. Piglets in the control group were orally administered with 2 mL 0.1 g/mL sterilized skim milk while the treatment group was administered the same volume of sterilized skim milk with the addition of viable L. rhamnosus at the 1st, 3rd, and 5th days after birth. The feeding trial was conducted for 25 d. Results showed that piglets in the L. rhamnosus group exhibited increased weaning weight and average daily weight gain, whereas diarrhea incidence was decreased. The bacterial abundance and composition of cecal contents, especially Firmicutes, Bacteroidetes, and Fusobacteria, were altered by probiotic treatment. In addition, L. rhamnosus increased the jejunal permeability and promoted the immunologic barrier through regulating antimicrobial peptides, cytokines, and chemokines via Toll-like receptors. Our findings indicate that oral administration of L. rhamnosus GG to newborn piglets is beneficial for intestinal health of pre-weaning piglets by improving the biological, physical, and immunologic barriers of intestinal mucosa.


Assuntos
Mucosa Intestinal/imunologia , Lactobacillus rhamnosus , Probióticos/administração & dosagem , Administração Oral , Animais , Animais Recém-Nascidos , Citocinas/genética , Feminino , Microbioma Gastrointestinal , Imunidade Inata , Masculino , Transdução de Sinais , Suínos , Desmame
16.
J Cancer Res Ther ; 14(Supplement): S1035-S1040, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30539842

RESUMO

Aim: Norcantharidin (NCTD) has been used as a clinical antineoplastic drug in China for several years, and diamminedichloroplatinum is a valuable clinical cancer chemotherapy agent. Here, we tried to investigate the effects of NCTD plus diamminedichloroplatinum on hepatic carcinoma in murine. Materials and Methods: In vivo and in vitro investigations on anticancer effects of the two drugs were individually made. Result: In vitro, the combination of the two drugs resulted in apparent apoptosis and cell proliferation inhibitions of H22 cancer cells. Meanwhile, their coadministration in vivo produced significant suppressions of tumor growth and cancerometastasis. Further, CD31 immunohistochemistry and matrigel tube formation assay demonstrated that angiogenesis was inhibited by NCTD plus diamminedichloroplatinum in vivo and in vitro, respectively. Conclusion: Based on the findings, we concluded that NCTD plus diamminedichloroplatinum may have an additive anticancer efficacy because the two drugs work in different ways, and thus, their combination had inhibited cancer cell proliferations and tumor angiogenesis more effectively than either of the compounds alone.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Carcinoma Hepatocelular/tratamento farmacológico , Cisplatino/farmacologia , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Pulmonares/tratamento farmacológico , Animais , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Apoptose/efeitos dos fármacos , Compostos Bicíclicos Heterocíclicos com Pontes/uso terapêutico , Carcinoma Hepatocelular/secundário , Linhagem Celular Tumoral/transplante , Proliferação de Células/efeitos dos fármacos , Cisplatino/uso terapêutico , Modelos Animais de Doenças , Sinergismo Farmacológico , Feminino , Humanos , Neoplasias Hepáticas/patologia , Neoplasias Pulmonares/secundário , Camundongos , Camundongos Endogâmicos BALB C , Neovascularização Patológica/tratamento farmacológico , Neovascularização Patológica/patologia , Resultado do Tratamento
17.
J Cancer ; 9(19): 3467-3478, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30310503

RESUMO

Background: We have previously reported the prognostic value of the albumin-to-alkaline phosphatase ratio (AAPR) for advanced hepatocellular carcinoma (HCC) patients who are not receiving any standard anticancer therapy. However, the prognostic value of the AAPR for HCC patients treated with trans-catheter arterial chemoembolization therapy (TACE) was not investigated. Methods: We retrospectively analysed 372 HCC patients treated with TACE (the training cohort) and applied receiver operating characteristic curves (ROC curves) to identify the best cut-off value for the AAPR in this cohort. Then, univariate analyses by the Kaplan-Meier method and multivariate analysis by a Cox proportional hazards regression model were conducted. Both comparisons of the ROC curves and the likelihood ratio test (LRT) were employed to evaluate the abilities of different factors in predicting the survival of patients in this cohort. Finally, the prognostic value of the AAPR was validated in two cohorts: one included 202 HCC patients treated with supportive care (validation cohort I), and the other included 82 HCC patients treated with TACE (validation cohort II). Results: We identified 0.439 as the best cut-off value of the AAPR by ROC curve analysis. An AAPR > 0.439 was significantly correlated with a lower frequency of Child-Pugh grade B, portal vein tumour thrombus (PVTT), T3-4 and lymph node metastasis (P < 0.05). The median overall survival (OS) of the patients with an AAPR > 0.439 was significantly longer than that of those with an AAPR ≤ 0.439 (58.4 m vs 17.8 m, respectively, P < 0.001). The AAPR was identified as an independent prognostic factor after univariate and multivariate analyses (HR = 0.636, P = 0.003). The independent prognostic value of the AAPR was also confirmed in validation cohorts I and II. Additionally, we substituted the AAPR for the Child-Pugh grade in the CLIP system and integrated the AAPR into the TNM system. We found that the area under the curve (AUC) of the AAPR-CLIP system was significantly larger than that of the CLIP and the TNM when predicting 3-month, 6-month, 1-year and 2-year survival (P < 0.05). There was no significant difference between the AUCs for the AAPR-CLIP and the AAPR-TNM. The LRT suggested that both AAPR-CLIP and AAPR-TNM had significantly larger χ2 values and smaller AIC values than that of their corresponding primary system (P < 0.05). Conclusions: The AAPR was an independent prognostic index for the HCC patients treated with TACE. Both AAPR-CLIP and AAPR-TNM outperformed their corresponding primary system in predicting OS in the current study.

18.
BMJ Open ; 8(9): e021768, 2018 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-30269064

RESUMO

OBJECTIVE: Type 2 diabetes mellitus is increasing in young adults, and greater adiposity is considered a major risk factor. However, whether there is an association between obesity and diabetes and how this might be impacted by age is not clear. Therefore, we investigated the association between body mass index (BMI) and diabetes across a wide range of age groups (20-30, 30-40, 40-50, 50-60, 60-70 and ≥70 years old). DESIGN: We performed a retrospective cohort study using healthy screening programme data. SETTING: A total of 211 833 adult Chinese persons >20 years old across 32 sites and 11 cities in China (Shanghai, Beijing, Nanjing, Suzhou, Shenzhen, Changzhou, Chengdu, Guangzhou, Hefei, Wuhan, Nantong) were selected for the study; these persons were free of diabetes at baseline. PRIMARY AND SECONDARY OUTCOME MEASURES: Fasting plasma glucose levels were measured and information regarding the history of diabetes was collected at each visit. Diabetes was diagnosed as fasting plasma glucose ≥7.00 mmol/L and/or self-reported diabetes. Patients were censored at the date of diagnosis or the final visit, whichever came first. RESULTS: With a median follow-up of 3.1 years, 4174 of the 211 833 participants developed diabetes, with an age-adjusted incidence rate of 7.35 per 1000 persons. The risk of incident diabetes increased proportionally with increasing baseline BMI values, with a 23% increased risk of incident diabetes with each kg/m2 increase in BMI (95% CI 1.22 to 1.24). Across all age groups, there was a linear association between BMI and the risk of incident diabetes, although there was a stronger association between BMI and incident diabetes in the younger age groups (age×BMI interaction, p<0.0001). CONCLUSIONS: An increased BMI is also independently associated with a higher risk of developing diabetes in young adults and the effects of BMI on incident diabetes were accentuated in younger adults.


Assuntos
Índice de Massa Corporal , Diabetes Mellitus Tipo 2/epidemiologia , Obesidade/epidemiologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Glicemia/metabolismo , China/epidemiologia , Diabetes Mellitus Tipo 2/diagnóstico , Jejum , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Adulto Jovem
19.
Curr Med Sci ; 38(2): 379-385, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30074200

RESUMO

This study aimed to explore the mechanism of a novel mutation (p.Lys38Glu) in apolipoprotein H (APOH) gene causing hereditary beta2-glycoprotein I (ß2GPI) deficiency and thrombosis in a proband with thrombophilia. The plasma level of ß2GPI was measured by ELISA and Western blotting, and anti-ß2GPI antibody by ELISA. Lupus anticoagulant (LA) was assayed using the dilute Russell viper venom time. Deficiency of the major natural anticoagulants including protein C (PC), protein S (PS), antithrombin (AT) and thrombomodulin (TM) was excluded from the proband. A mutation analysis was performed by amplification and sequencing of the APOH gene. Wild type and mutant (c.112A>G) APOH expression plasmids were constructed and transfected into HEK293T cells. The results showed that the thrombin generation capacity of the proband was higher than that of the other family members. Missense mutation p.Lys38Glu in APOH gene and LA coexisted in the proband. The mutation led to ß2GPI deficiency and thrombosis by impairing the protein production and inhibiting the platelet aggregation. It was concluded that the recurrent thrombosis of the proband is associated with the coexistence of p.Lys38Glu mutation in APOH gene and LA in plasma.


Assuntos
Mutação/genética , Trombofilia/etiologia , Trombofilia/genética , beta 2-Glicoproteína I/deficiência , beta 2-Glicoproteína I/genética , Sequência de Aminoácidos , Sequência de Bases , Biologia Computacional , Demografia , Família , Feminino , Células HEK293 , Humanos , Masculino , Linhagem , Agregação Plaquetária , Ligação Proteica , Proteínas Recombinantes/metabolismo , Ressonância de Plasmônio de Superfície , Trombina/metabolismo , Trombofilia/sangue , Adulto Jovem , beta 2-Glicoproteína I/sangue , beta 2-Glicoproteína I/química
20.
Cell Death Dis ; 9(9): 843, 2018 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-30143606

RESUMO

Following publication of the article, the authors reported that Huixiong Xu had left the department, and requested that the author list be amended accordingly.

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