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1.
Open Life Sci ; 16(1): 728-736, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34316513

RESUMO

Osteosarcoma is the most common type of primary malignant tumor of the bone, with a high metastatic rate and poor prognosis. Therefore, it is important to further elucidate the molecular mechanisms involved in the development of osteosarcoma and explore new molecular therapeutic targets. Long intergenic nonprotein-coding RNA 707 (LINC00707) is an oncogenic gene in several cancers. In this study, we further clarified its role and regulatory mechanism in osteosarcoma. We found that LINC00707 levels are significantly higher in the osteosarcoma cell lines SW 1353, HOS, U-2 OS, MG-63, and Saos-2 compared to those in human fetal osteoblastic cell line hFOB1.19. LINC00707 silencing suppressed cell proliferation, migration, and invasion of MG-63 and Saos-2 cells. Moreover, LINC00707 can act as a competitive endogenous RNA of miR-338-3p, and miR-338-3p inhibitor and AHSA1 overexpression alleviated the effect of LINC00707 silencing. In conclusion, we demonstrated high expression of LINC00707 in osteosarcoma cell lines and that silencing LINC00707 suppresses cell proliferation, migration, and invasion by targeting the miR-338-3p/AHSA1 axis in MG-63 and Saos-2 cells. These findings suggest that LINC00707 may serve as a potential target for osteosarcoma treatment.

2.
Ann Clin Transl Neurol ; 8(1): 266-270, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33314640

RESUMO

Biallelic mutations in the sorbitol dehydrogenase (SORD) encoding gene were recently identified as a common genetic cause in autosomal-recessive CMT patients. Here, we investigated the clinical, genetic, and electrophysiological characteristics of three CMT patients with biallelic SORD mutations from a Chinese cohort. Two patients harbored c.757delG (p.A253Qfs*27) homozygous mutations, and one patient carried both c.757delG (p.A253Qfs*27) and c.625C>T (p.R209X) compound heterozygous mutations. Interestingly, the two patients homozygous for the c.757delG mutation exhibited positive responses for pinprick test. In conclusion, we confirmed SORD mutations as causative for CMT and further expanded the mutational and phenotypic spectrum of SORD-related CMT.


Assuntos
Doença de Charcot-Marie-Tooth/genética , L-Iditol 2-Desidrogenase/genética , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Feminino , Humanos , Masculino , Mutação , Adulto Jovem
3.
Clin Ophthalmol ; 14: 2185-2193, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32801629

RESUMO

Purpose: To evaluate the effects of the vascular endothelial growth factor inhibitor conbercept (KH902) on corneal neovascularization and wound healing following penetrating keratoplasty in rabbits. Methods: Conbercept was administered to New Zealand white rabbits through topical and subconjunctival routes. Corneal neovascularization and wound healing were examined by slit-lamp photography and histological analyses. The expressions of vascular endothelial growth factor inhibitor, α-smooth muscle actin, and keratocan in the corneal grafts were measured by real-time quantitative polymerase chain reaction (RT-qPCR). Results: The anterior segment photographs demonstrated that corneal neovascularization started in the 2nd week. In the 4th week, histologically, the superficial corneal stroma layer showed disordered arrangement, and there were large numbers of dense inflammatory cells and blood vessels in the stroma layer. Vascular endothelial growth factor in the experimental groups was significantly decreased at all time points compared with the control group (both P = 0.001). Expression of α-smooth muscle actin in corneal grafts demonstrated an increase in time even it was lower in experimental groups, but the difference was not statistically significant (P equaled to 0.507 and 0.723, respectively). There were no significant differences with the expression of keratocan in all groups except that it significantly declined at the 4th week as to the second week in all groups and P values were 0.022, 0.020 and 0.014 in control (C), topical (E1), and subconjunctival (E2) group, respectively. Conclusion: The study found that conbercept inhibited the formation of corneal neovascularization without affecting keratocan-mediated corneal wound healing and there were no significant differences between topical administration of different doses of conbercept on the rabbit corneal neovascularization after penetrating keratoplasty in this study.

4.
Curr Eye Res ; 45(12): 1467-1476, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32631094

RESUMO

Purpose: To provide a detailed review on the therapeutic efficacy of conbercept for the management of ocular vasculopathies. Methods: A comprehensive literature search of various electronic databases was performed. Results: Ocular vasculopathy is one of the major causes of visual impairment and blindness which includes a range of disorders. Vascular endothelial growth factor (VEGF) regulates angiogenesis, enhances vascular permeability, and drives the formation of neovascularization. Anti-VEGF therapy has been shown to prevent vision loss or potentially improve vision in patients with exudative or neovascular retinal disease. The most recent anti-VEGF drug in China is conbercept. In the USA and Europe, bevacizumab is the most recently approved anti-VEGF agent. Conclusions: Conbercept serves as another anti-VEGF option for patients with neovascular AMD and other retinal vascular disorders. There have not been many clinical trials that study conbercept as compared with other currently available anti-VEGF drugs. There is a need for large-scale, well-designed, randomized clinical trials to ensure its long-term safety and efficacy and to determine if it has any advantages over other anti-VEGF agents.


Assuntos
Inibidores da Angiogênese/uso terapêutico , Neovascularização de Coroide/tratamento farmacológico , Neovascularização da Córnea/tratamento farmacológico , Retinopatia Diabética/tratamento farmacológico , Glaucoma Neovascular/tratamento farmacológico , Proteínas Recombinantes de Fusão/uso terapêutico , Oclusão da Veia Retiniana/tratamento farmacológico , Humanos , Injeções Intravítreas , Degeneração Macular/tratamento farmacológico , Miopia Degenerativa/tratamento farmacológico , Fator A de Crescimento do Endotélio Vascular/antagonistas & inibidores
5.
Bioorg Chem ; 99: 103814, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32278208

RESUMO

A new anticancer N-containing heterocyclic scaffold was designed and 30 pyridazino[1,6-b]quinazolinone derivatives were synthesized and characterized. Antiproliferation evaluation in vitro against four human cancer cell lines including SK-OV-3(ovarian cell), CNE-2(nasopharyngeal cell), MGC-803(gastric cell) and NCI-H460(lung cell) indicated that most of them exhibited potent anticancer activity and the IC50 value of the most potent compound lowered to sub-µM. DNA interaction assay indicated that compounds 4e, 4g, 6o, 6p, 8o can intercalate into DNA. Compounds 6 and 8 also demonstrated potent topoisomerase I (topo I) activity. Annexin V- FITC/propidium iodide dual staining assay and cell cycle analysis indicated that 2-(4-bromophenyl)-4-((3-(diethylamino)propyl)amino) -10H-pyridazino [1,6-b]quinazolin- 10-one (8p) could induce arrest cell cycle at G2 phase and apoptosis in MGC-803 cells in a dose-dependent manner. The in vivo antitumor efficiency of compound 8p was also evaluated on MGC-803 xenograft nude mice, and the relative tumor growth inhibition was up to 55.9% at a dose of 20 mg/kg per two days. The results suggested that pyridazino[1,6-b]-quinazolinones might serve as a promising novel scaffold for the development of new antitumor agents.


Assuntos
Antineoplásicos/farmacologia , DNA Topoisomerases Tipo I/metabolismo , DNA/química , Quinazolinonas/farmacologia , Inibidores da Topoisomerase I/farmacologia , Animais , Antineoplásicos/síntese química , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Bovinos , Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Simulação de Acoplamento Molecular , Estrutura Molecular , Quinazolinonas/síntese química , Quinazolinonas/química , Relação Estrutura-Atividade , Inibidores da Topoisomerase I/síntese química , Inibidores da Topoisomerase I/química , Células Tumorais Cultivadas
6.
Eye (Lond) ; 34(9): 1600-1607, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-31784702

RESUMO

BACKGROUND/OBJECTIVES: To evaluate the clinical efficacy of topical tacrolimus 0.1% and cyclosporine 1% on high-risk penetrating keratoplasty (PKP) patients. SUBJECTS/METHODS: A series of 49 high-risk PKP patients (49 eyes), 20 males, 29 females from the age of 4 months to 74 years of age with the mean of 32.5 from 2012 to 2017 were recruited in this study. The patients were randomly divided into two groups by receiving either topical tacrolimus 0.1% or cyclosporine 1% respectively. Twenty five patients were treated with topical tacrolimus 0.1% and 24 patients with topical cyclosporine 1%. The traditional baseline management on these two groups were Tobramycin and Dexamethasone eye drops in the first 3 weeks and then tapered off. Clinical procedures and postoperative follow-up were documented. RESULTS: After 6-54 months follow-up, with the average of 24 months, 11 of 24 high-risk patients (11 eyes) had graft rejection, the rejection rate was 45.8% in topical cyclosporine 1% group. The rejections occurred from 35 days to 20 months after PKP. Three patients had irreversible rejection. On topical tacrolimus 0.1% group, the rejection occurred in four patients (four eyes) with rejection rate of 16%, and no irreversible rejection was observed. The graft rejection episodes were documented between 23 days and 24 months. As compared with the topical cyclosporine 1%, topical tacrolimus 0.1%, a key immunosuppressant, significantly decreased corneal graft rejection rate (p = 0.02). CONCLUSIONS: Topical tacrolimus 01% on high-risk PKP patients significantly prevented corneal graft rejection, and it had less adverse effects and was very safe to high-risk patients as to topical cyclosporine 1%. Further case controlled randomized clinical trial studies are needed to establish the best management option for these high-risk patients.


Assuntos
Ceratoplastia Penetrante , Tacrolimo , Ciclosporina , Feminino , Rejeição de Enxerto/prevenção & controle , Humanos , Imunossupressores , Masculino , Resultado do Tratamento
7.
Curr Med Sci ; 40(6): 1161-1169, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33428145

RESUMO

Estrogen deficiency, which mainly occurs in postmenopausal women, is a primary reason for osteoporosis in clinical diagnosis. However, the molecular regulation of osteoporosis in menopausal females is still not adequately explained in the literature, with the diagnosis and treatment for osteoporosis being limited. Herein, exosomal microRNAs (miRNAs) were used to evaluate their diagnosis and prediction effects in menopausal females with osteoporosis. In this study, 6 menopausal females without osteoporosis and 12 menopausal females with osteoporosis were enrolled. The serum exosomes were isolated, and the miRNA expression was detected by miRNA high-throughput sequencing. Exosomal miRNA effects were analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. The miRNA-targeted genes were evaluated by Targetscan 7.2 and the protein-protein interactions (PPI) by STRING. Hub genes were analyzed by the CytoHubba app of Cytoscape. The results showed that 191 aberrant miRNAs were found in the group of menopausal females with osteoporosis, including 72 upregulated miRNAs and 121 downregulated miRNAs. Aberrant miRNAs were involved in many signaling pathways, such as the Wnt, MAPK, and Hippo pathways. Based on PPI network analysis, FBXL3, FBXL13, COPS2, UBE2D3, DCUN1D1, DCUN1D4, CUL3, FBXO22, ASB6, and COMMD2 were the 10 most notable genes in the PPI network. In conclusion, aberrant serum exosomal miRNAs were associated with an altered risk of osteoporosis in menopausal females and may act as potential biomarkers for the prediction of risk of osteoporosis in menopausal females.


Assuntos
Biomarcadores/sangue , Exossomos/genética , Perfilação da Expressão Gênica/métodos , Menopausa/genética , MicroRNAs/genética , Osteoporose/diagnóstico , Estudos de Casos e Controles , Diagnóstico Precoce , Feminino , Regulação da Expressão Gênica , Redes Reguladoras de Genes , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Menopausa/sangue , Pessoa de Meia-Idade , Osteoporose/sangue , Osteoporose/genética , Mapas de Interação de Proteínas , Análise de Sequência de RNA
8.
Onco Targets Ther ; 11: 6957-6967, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30410353

RESUMO

Background: Basal cell carcinoma (BCC) is a frequent malignant tumor of skin cancers with high morbidity. The objective of this study was to identify critical genes and pathways related to the carcinogenesis of BCC and gain more insights into the underlying molecular mechanisms of BCC. Materials and methods: The gene expression profiles of GSE7553 and GSE103439 were downloaded from the Gene Expression Omnibus database with 19 tumors and 6 normal skin tissues. Differentially expressed genes (DEGs) were screened between BCC samples and normal tissues, followed by gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Subsequently, protein-protein interaction (PPI) network was constructed for these DEGs, and module analysis was performed. Results: A total of 313 DEGs were obtained. Among them, 222 genes were upregulated and 91 genes were downregulated. Enrichment analysis indicated that the upregulated genes were significantly enriched in cell cycle and mitosis, while the downregulated genes were mainly associated with unsaturated fatty acid metabolic process and cell differentiation. In addition, TOP2A, CDK1, and CCNB1 were identified as the top three hub genes ranked by degrees in the PPI network. Meanwhile, three subnetworks were derived, which indicated that these DEGs were significantly enriched in pathways, including "cell cycle", "extracellular matrix-receptor interaction", "basal cell carcinoma", and "hedgehog signaling pathway". Conclusions: The novel critical DEGs and pathways identified in this study may serve pivotal roles in the carcinogenesis of BCC and indicate more molecular targets for the treatment of BCC.

9.
Biochemistry (Mosc) ; 81(6): 591-9, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27301287

RESUMO

Cadherin is an epidermal growth factor and laminin-G seven-pass G-type receptor 1 (CELSR1) is a key component of the noncanonical Wnt/planar cell polarity (PCP) pathway that critically regulates endothelial cell proliferation and angiogenesis. In this study, we examined the biological significance of CELSR1 in endothelial cell migration and angiogenesis. For this, we applied both gain-of-function and loss-of-function approaches. To increase the endogenous expression of CELSR1, we used the transcription activator-like effector (TALE) technology and constructed an artificial TALE-VP64 activator. To knock down the expression of CELSR1, we generated lentivirus containing short hairpin RNA sequences targeting different regions of CELSR1 mRNA. Following up- or down-regulation of CELSR1 in human aortic endothelial cells (HAEC), we assessed in vitro cell proliferation by MTT assay, migration by scratch and transwell migration assays, and angiogenesis by tube formation analysis. We found that CELSR1 was endogenously expressed in human umbilical vein endothelial cells (HUVEC) and HAEC. When focusing on HAEC, we found that upregulating CELSR1 expression significantly promoted cell growth, while knocking down CELSR1 inhibited the growth (p < 0.05). Using both scratch and transwell migration assays, we observed a positive correlation between CELSR1 expression and cell migratory capability. In addition, CELSR1 upregulation led to higher levels of tube formation in HAEC, while downregulating CELSR1 expression decreased tube formation (p < 0.05). Mechanistically, CELSR1-regulated migration and tube formation was mediated through disheveled segment polarity protein 3 (Dvl3). In conclusion, CELSR1 plays an important role in regulating multiple phenotypes of endothelial cells, including proliferation, migration, and formation of capillary-like structures.


Assuntos
Caderinas/metabolismo , Células Endoteliais/citologia , Neovascularização Fisiológica/genética , Caderinas/antagonistas & inibidores , Caderinas/genética , Linhagem Celular , Movimento Celular/genética , Proliferação de Células , Células Endoteliais/metabolismo , Células Endoteliais da Veia Umbilical Humana , Humanos , Plasmídeos/genética , Plasmídeos/metabolismo , Interferência de RNA , RNA Interferente Pequeno/metabolismo
10.
Sci Rep ; 6: 24721, 2016 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-27097866

RESUMO

Newcastle disease virus (NDV) can replicate and trigger autophagy in human tumor cells. Our previous study confirmed the critical role of autophagy in NDV infection. Here we studied the role of NDV structural proteins in the induction of autophagy through endoplasmic reticulum (ER) stress-related unfolded protein response (UPR) pathways. Ectopic expression of the NDV nucleocapsid protein (NP) or phosphoprotein (P) was sufficient to induce autophagy. NP or P expression also altered ER homeostasis. The PERK and ATF6 pathways, but not the XBP1 pathway, all of which are components of the UPR, were activated in both NDV-infected and NP or P-transfected cells. Knockdown of PERK or ATF6 inhibited NDV-induced autophagy and reduced the extent of NDV replication. Collectively, these data suggest not only roles for the NDV NP and P proteins in autophagy, but also offer new insights into the mechanisms of NDV-induced autophagy through activation of the ER stress-related UPR pathway.


Assuntos
Autofagia , Estresse do Retículo Endoplasmático , Vírus da Doença de Newcastle/fisiologia , Nucleoproteínas/metabolismo , Fosfoproteínas/metabolismo , Resposta a Proteínas não Dobradas , Proteínas Virais/metabolismo , Fator 6 Ativador da Transcrição/metabolismo , Autofagia/genética , Linhagem Celular Tumoral , Expressão Gênica , Humanos , Proteínas do Nucleocapsídeo , Nucleoproteínas/genética , Fagossomos/metabolismo , Fosfoproteínas/genética , Splicing de RNA , RNA Mensageiro/genética , RNA Interferente Pequeno/genética , Transdução de Sinais , Transfecção , Proteínas Virais/genética , Replicação Viral , Proteína 1 de Ligação a X-Box/genética , eIF-2 Quinase/metabolismo
11.
J Cell Sci ; 128(21): 3977-89, 2015 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-26395397

RESUMO

Eukaryotic initiation factor 6 (eIF6) is a pivotal regulator of ribosomal function, participating in translational control. Previously our data suggested that eIF6 acts as a key binding protein of P311 (a hypertrophic scar-related protein; also known as NREP). However, a comprehensive investigation of its functional role and the underlying mechanisms in modulation of myofibroblast (a key effector of hypertrophic scar formation) differentiation remains unclear. Here, we identified that eIF6 is a novel regulator of transforming growth factor-ß1 (TGF-ß1) expression at transcription level, which plays a key role in myofibroblast differentiation. Mechanistically, this effect is associated with eIF6 altering the occupancy of the TGF-ß1 promoter by H2A.Z (Swiss-Prot P0C0S6) and Sp1. Accordingly, modulation of eIF6 expression in myofibroblasts significantly affects their differentiation via the TGF-ß/Smad signaling pathway, which was verified in vivo by the observation that heterozygote eIF6(+/-) mice exhibited enhanced TGF-ß1 production coupled with increased α-smooth muscle actin (α-SMA)(+) myofibroblasts after skin injury. Overall, our data reveal a novel transcriptional regulatory mechanism of eIF6 that acts on facilitating Sp1 recruitment to TGF-ß1 promoter via H2A.Z depletion and thus results in increased TGF-ß1 transcription, which contributes to myofibroblast differentiation.


Assuntos
Diferenciação Celular/genética , Miofibroblastos/citologia , Miofibroblastos/metabolismo , Fatores de Iniciação de Peptídeos/metabolismo , Fator de Transcrição Sp1/metabolismo , Fator de Crescimento Transformador beta1/genética , Animais , Diferenciação Celular/fisiologia , Células Cultivadas , Camundongos , Camundongos Mutantes , Fatores de Iniciação de Peptídeos/genética , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Fator de Transcrição Sp1/genética
12.
Shanghai Kou Qiang Yi Xue ; 24(1): 76-82, 2015 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-25858374

RESUMO

PURPOSE: In this study, micro-implants were used in 15 adult patients with mild and moderate bimaxillary arch protrusion or crowding. Cephalometric analysis was used to analyze hard and soft-tissues change before and after treatment, with the aim to investigate the effects of treatment on adult bimaxillary arch protrusion with micro-implant anchorage. METHODS: Fifteen adult patients with mild and moderate bimaxillary arch protrusion were selected in this study. Micro-implants were inserted into the zygomaticoalveolar ridge of maxilla and the external oblique line of mandible. A NiTi coil spring was attached to the micro-implant to drag the whole upper and lower dentition for distal movement. Cephalometrics were taken before and after treatment, and the changes of soft and hard-tissue profile were studied. SPSS13.0 software package was used to analyze the data. RESULTS: (1)Sixty micro-implants remained stable.(2)SNA, SNB had no significant changes (P>0.05), and the relationship between the maxilla and the mandible did not change significantly. U1/NA, U1-NA, L1/NB, L1-NB and U1/L1 changes in hard tissue had significant difference in cephalometric measurement (P<0.05). The upper and lower anterior teeth were more retrusive, and the tipping of incisor decreased significantly.(3)Cephalometric analysis showed that lateral appearance improved and soft tissue cephalometric-related measurements such as Cm-Sn-UL,LL-B'-Pos increased significantly (P<0.01). (4)Molars and incisors acquired distal movement. CONCLUSIONS: Micro-implant can provide not only excellent skeletal anchorage but also a novel way to distalize the whole dentition efficiently.


Assuntos
Cefalometria , Procedimentos de Ancoragem Ortodôntica , Ortodontia Corretiva , Sobremordida , Adulto , Humanos , Incisivo , Má Oclusão , Mandíbula , Maxila , Dente Molar
13.
Gene ; 550(1): 148-53, 2014 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-25106856

RESUMO

Fecundity improvement is one of the most important objectives for goat breeders as it greatly increases production efficiency. To investigate the genes associated with litter sizes in the Anhui White goat (AWG), gene expression differences in the ovaries of uniparous and multiparous AWG were assessed using the RNA-Seq (Quantification) method. This analysis generated 6,027,714 and 5,884,062 clean reads in uniparous and multiparous libraries, respectively. A total of 2201 differentially expressed genes (DEGs) were thereby identified (FDR≤0.001, |log2Ratio|≥1). There were 1583 up-regulated and 618 down-regulated genes in the multiparous samples compared with the uniparous samples. A large number of these DEGs were related to the terms cellular process, cell & cell part and binding. Twelve genes which may be associated with the high prolificacy of AWG were identified using a bioinformatic screen. In addition, pathway analysis revealed that these DEGs were significantly enriched in 11 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including ECM-receptor interactions, focal adhesion, and regulation of the actin cytoskeleton among others. This suggested a role for these pathways in the prolificacy of AWG. These results provide a list of new candidate genes for goat prolificacy.


Assuntos
Perfilação da Expressão Gênica/veterinária , Cabras/genética , Ovário/metabolismo , Análise de Sequência de RNA/métodos , Animais , Feminino , Ontologia Genética , Redes Reguladoras de Genes , Modelos Genéticos , Paridade , Gravidez , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transdução de Sinais/genética
14.
BMC Genomics ; 15: 339, 2014 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-24886377

RESUMO

BACKGROUND: Superior kidding rate is an important economic trait in production of meat goat, and ovulation rate is the precondition of kidding rate. MicroRNAs (miRNAs) play critical roles in almost all ovarian biological processes, including folliculogenesis, follicle development, follicle atresia, luteal development and regression. To find out the different ovarian activity and follicle recruitment with miRNA-mediated posttranscriptional regulation, the small RNAs expressed pattern in the ovarian tissues of multiple and uniparous Anhui White goats during follicular phase was analyzed using Solexa sequencing data. RESULTS: 1008 miRNAs co-expressed, 309 and 433 miRNAs specifically expressed in the ovaries of multiple and uniparous goats during follicular phase were identified. The 10 most highly expressed miRNAs in the multiple library were also the highest expressed in the uniparous library, and there were no significantly different between each other. The highest specific expressed miRNA in the multiple library was miR-29c, and the one in the uniparous library was miR-6406. 35 novel miRNAs were predicted in total. GO annotation and KEGG Pathway analyses were implemented on target genes of all miRNA in two libraries. RT-PCR was applied to detect the expression level of 5 randomly selected miRNAs in multiple and uniparous hircine ovaries, and the results were consistent with the Solexa sequencing data. CONCLUSIONS: In the present study, the different expression of miRNAs in the ovaries of multiple and uniparous goats during follicular phase were characterized and investigated using deep sequencing technology. The result will help to further understand the role of miRNAs in kidding rate regulation and also may help to identify miRNAs which could be potentially used to increase hircine ovulation rate and kidding rate in the future.


Assuntos
Fase Folicular , Cabras/genética , MicroRNAs/metabolismo , Ovário/metabolismo , Animais , Feminino , MicroRNAs/genética , Reação em Cadeia da Polimerase , Processamento Pós-Transcricional do RNA
15.
Zhonghua Yi Xue Za Zhi ; 93(33): 2664-6, 2013 Sep 03.
Artigo em Chinês | MEDLINE | ID: mdl-24360049

RESUMO

OBJECTIVE: To explore the clinical efficacy of endoscopic repairing pediatric blowout fracture of orbital floor with autologous bone fragment. METHODS: A total of 12 cases with blowout fractures of orbital floor between March 2010 and June 2012 at Third Hospital of HeBei Medical University were reviewed. They underwent nasal endoscopic reconstruction of autologous bone fragment in situ. Medicinal biomaterial glue was used for fixation. A 6-month follow-up was performed and the therapeutic efficacies of anatomic and functional recovery were evaluated. RESULTS: No further vision loss or infection occurred postoperatively. Diplopia disappeared over a period of 3 days to 6 months. At 3 months post-operation, diplopia vanished completely (n = 9), and peripheral vision diplopia persisted (n = 3). Only 1 case had still peripheral vision diplopia at 6 months. Enophthalmos: ≥ 2 mm (n = 1), 1 mm (n = 3) for one week after surgery and only 1 mm (n = 1) at one month follow-up. Passive traction test was negative in all cases and computed tomograph (CT) revealed no bone redislocation postoperatively. CONCLUSION: Endoscopic repair of pediatric blowout fracture of orbital floor with autologous bone fragment in situ is both effective and safe.


Assuntos
Transplante Ósseo/métodos , Endoscopia/métodos , Órbita/lesões , Fraturas Orbitárias/cirurgia , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Cavidade Nasal/cirurgia , Estudos Retrospectivos , Transplante Autólogo
16.
Headache ; 53(10): 1595-601, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24021092

RESUMO

OBJECTIVE: Recent genome-wide association studies (GWAS) have identified 3 loci in or near PRDM16 (1p36.32, rs2651899), LRP1 (12q13.3, rs11172113) and TRPM8 (2q37.1, rs10166942) in the population-based Women's Genome Health Study (WGHS) of migraine, and 2 loci in or near TRPM8 and LRP1 were repeated in European GWAS study. To evaluate whether the same variants are related to migraine in Chinese population, we investigated migraine with aura (MA) and migraine without aura (MO) patients of Chinese Han ethnicity in mainland China. METHODS: A case-control study in a cohort of 207 migraine cases and 205 ethnically matched controls was conducted by using the dual-color fluorescence resonance energy transfer (FRET) probes analysis. RESULTS: The genotypes of all polymorphisms in 2 groups followed the Hardy-Weinberg equilibrium. We found significant differences in allele distribution of rs2651899 variant in PRDM16 between MO patients and control subjects (P = .049, OR = 1.335, 95%CI 1.001-1.782), and there were no difference between MA patients and controls in the frequency of genotype and allele. Also, no significant differences in genotypic and allelic distributions between MA or MO patients and controls were observed in the polymorphisms of rs10166942 of TRPM8 and rs11172113 of LRP1, and there was no significant difference comparing male with female in all loci. CONCLUSION: Our data suggested that rs2651899 variant in PRDM16 plays a potential role in Chinese MO migraine susceptibility, and gender may not play a role.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Proteínas de Ligação a DNA/genética , Predisposição Genética para Doença/genética , Variação Genética/genética , Transtornos de Enxaqueca/diagnóstico , Transtornos de Enxaqueca/genética , Fatores de Transcrição/genética , Adulto , Grupo com Ancestrais do Continente Asiático/etnologia , Estudos de Casos e Controles , Estudos de Coortes , Feminino , Seguimentos , Predisposição Genética para Doença/etnologia , Humanos , Masculino , Pessoa de Meia-Idade , Transtornos de Enxaqueca/etnologia , Fatores de Risco , Adulto Jovem
17.
Zhonghua Shao Shang Za Zhi ; 29(3): 267-71, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24059952

RESUMO

OBJECTIVE: To study the effects of antisense p38α mitogen-activated protein kinase (hereinafter referred to as p38α) on myocardial cells exposed to hypoxia and burn serum. METHODS: Thirty adult SD rats were inflicted with 40% TBSA full-thickness burn on the back to obtain burn serum. The myocardial cells were isolated from 80 neonatal SD rats and cultured, then they were divided into 4 groups according to the random number table: normal control group (N, ordinary culture without any treatment), hypoxia+burn serum group (HB, exposed to hypoxia after being treated with 10% burn rat serum), hypoxia+burn serum+infection group (HBI, exposed to hypoxia and 10% burn rat serum after being infected with antisense p38α gene-carrying adenovirus), hypoxia+burn serum+empty vector infection group (exposed to hypoxia and 10% burn rat serum after being infected with adenovirus empty vector). At post hypoxia hour (PHH) 1, 3, 6, and 12, mRNA and protein expression levels of p38α in the latter 3 groups were determined by RT-PCR and Western blotting, cell viability was determined by methylthianolyldiphenyl-tetrazolium bromide assay, and lactate dehydrogenase (LDH) activity was assayed at the same time point. At PHH 1, 6, and 12, apoptosis rate of myocardial cells was assessed by annexin V staining method. The indexes of group N were determined with the methods mentioned-above. Three wells were set at each time point in each group. Data were processed with one-way analysis of variance and LSD- t test. RESULTS: (1) At PHH 1, 3, and 6, the p38α mRNA level was higher in group HB than in group N and group HBI (with t values from 2.725 to 4.375, P values all below 0.05). (2) At PHH 1, 3, and 6, the p38α protein level was higher in group HB than those in group N and group HBI (with t values from 5.351 to 7.981, P values all below 0.01). (3) At PHH 3, 6, and 12, the cell viability in group HB (0.115 ± 0.007, 0.104 ± 0.006, 0.094 ± 0.005) was lower than that in group N (0.141 ± 0.014) and group HBI (0.136 ± 0.009, 0.124 ± 0.010, 0.112 ± 0.007, with t values from 2.357 to 6.812, P values all below 0.05). (4) The LDH activity was up-regulated in group HB as compared with that in group N and group HBI at each time point (with t values from 22.753 to 201.273, P values all below 0.01). (5) At PHH 1, 6, and 12, the apoptosis rate of myocardial cells in group HB [(5.4 ± 0.7)%, (8.7 ± 1.1)%, (13.6 ± 1.7)%] was higher than that of group N [(3.1 ± 0.3)%] and group HBI [(4.3 ± 0.5)%, (5.1 ± 0.7)%, (7.2 ± 0.9)%, with t values from 2.345 to 9.700, P < 0.05 or P < 0.01]. CONCLUSIONS: Antisense p38α can protect the myocardial cells from the injury of hypoxia and burn serum.


Assuntos
Elementos Antissenso (Genética)/genética , Proteína Quinase 14 Ativada por Mitógeno/genética , Miócitos Cardíacos/metabolismo , Animais , Apoptose , Hipóxia Celular , Células Cultivadas , Feminino , Masculino , Proteína Quinase 14 Ativada por Mitógeno/metabolismo , Miócitos Cardíacos/patologia , Ratos , Ratos Sprague-Dawley , Soro , Transfecção
18.
Shanghai Kou Qiang Yi Xue ; 22(3): 252-9, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-23852051

RESUMO

PURPOSE: To demonstrate the histological changes of the maxilla-facial sutures caused by retractive forces of a zygomatic implant anchorage to the maxilla of rhesus monkeys. METHODS: Four young male rhesuses were employed, 3 in the experimental group (1 was been distracted for 1.5 months and 2 for 3 months), and 1 as control. HE staining and vital fluorescent were used to observe histological changes in the circum-maxillary sutures (i.e. zygomaticomaxillary suture, transverse palatine suture, frontomaxillary suture and pterygopalatine suture) and the posterior sutures (i.e. zygomaticotemporal suture and sphenozygomatic suture, sphenozygomatic suture) of zygoma bone caused by retractive forces of zygomatic implant to the maxilla of rhesus monkeys. RESULTS: Obvious reactions of compressive stress and bone resorption were observed around the 4 circum-maxillary sutures. There were 3 patterns of sutural reactions, indicating the direction of sutural stress influenced by sutural position and morphology. Reactions of interdigitate wedging were observed in the transverse palatine suture and frontomaxillary suture, showing a bony collision that the tip of bony process and the hollow were resorbed markedly. Phasic reactions were found in the pterygopalatine suture reaction, representing a bony sliding that one side of a bony surface was deposited while the opposite side was resorptive. Both reactions were observed in the zygomaticomaxillary suture. Tensile stresses were observed in the 3 sutures around anchoraged zygoma bone (i.e. zygomaticotemporal suture, sphenozygomatic suture and sphenozygomatic suture) with similar three-pattern reactions. CONCLUSIONS: Histologically, significant changes differing from physiological remodeling are observed in the circum-maxillary sutures and posterior sutures of zygoma bone, but no destructive changes are found. These findings demonstrate that bone resorptions are major reactions caused in the 4 circum-maxillary sutures. Different sutures respond slight histological differences. The maxillary complex is retracted distally with some rotation. Tensile stress occured in the posterior sutures around zygoma bone with a zygomatic implant anchorage, which is different from the effect with a headgear. Supported by Science and Technology Plan Project of Yunnan Province (2007C0029R).


Assuntos
Suturas Cranianas , Implantes Dentários , Maxila , Animais , Face , Macaca mulatta , Masculino , Zigoma
19.
Neurosci Lett ; 549: 78-81, 2013 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-23811028

RESUMO

A number of genes have been implicated in the pathogenesis of migraine, a common neurological disorder also in China. However, data on association of genetic variations with migraine susceptibility among Chinese, which might be different from people of other ethnic background, are still scarce. We have therefore investigated the association of polymorphisms in four genes, MTHFR C677T, ACE I/D, MAOA T941G and TNF-ß G252A, which are considered to be with risk of migraine. A case-control study including a cohort of 151 migraine cases and 137 ethnically matched controls was conducted. The genotypes of each polymorphism followed the Hardy-Weinberg equilibrium in the two groups. Genotypic distribution of MTHFR C677T was significantly different with higher frequency of allele T in the migraine cohort as compared with that in controls (OR=1.686, 95%CI: 1.175-2.420, P=0.004). No difference was found between migraine with aura (MA) patients and controls, but T allele frequency was significantly higher in migraine without aura (MO) than in controls (OR=1.744, 95% CI: 1.202-2.532, P=0.003). No difference in genotypic and allelic distributions was observed between migraine patients and controls for the other polymorphisms, including ACE I/D, MAOA T941G, and TNF-ß G252A. Our data suggested that MTHFR C677T polymorphism plays a role in Chinese migraine susceptibility, especially in MO.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Predisposição Genética para Doença , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Transtornos de Enxaqueca/genética , Adulto , Alelos , Estudos de Casos e Controles , China , Feminino , Frequência do Gene , Estudos de Associação Genética , Genótipo , Humanos , Linfotoxina-alfa/genética , Masculino , Pessoa de Meia-Idade , Monoaminoxidase/genética , Polimorfismo de Nucleotídeo Único
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