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1.
Cancer Med ; 9(2): 615-625, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31793749

RESUMO

BACKGROUND: Models for estimation of survival rates of patients with intracranial grade II/III ependymoma (EPN) are scarce. Considering the heterogeneity in prognostic factors between pediatric and adult patients, we aimed to develop age-specific nomograms for predicting 3-, 5-, and 8-year survival for these patients. METHODS: A total of 1390 cases (667 children; 723 adults) of intracranial grade II/III EPNs diagnosed between 1988 and 2015 were extracted from the Surveillance, Epidemiology, and End Results (SEER) database for our study. Univariable and multivariable Cox analyses were employed to identify independent prognostic predictors. Age-specific nomograms were developed based on the results of multivariate Cox analyses. We also evaluated the performance of these predictive models by concordance index, calibration curves, time-dependent receiver operating characteristic curves, and decision curve analyses. RESULTS: Considerable heterogeneity in prognostic factors was highlighted between pediatric and adult patients. Age, sex, tumor grade, surgery treatment and radiotherapy were identified as significant predictors of overall survival for children, and age, tumor grade, tumor size, surgery treatment, and marital status for adult. Based on these factors, age-specific nomogram models were established and internally validated. These models exhibited favorable discrimination and calibration characteristics. Nomogram-based risk classification systems were also constructed to facilitate risk stratification in EPNs for optimization of clinical management. CONCLUSIONS: We developed the first nomograms and corresponding risk classification systems for predicting survival in patients with intracranial grade II/III EPN. These easily used tools can assist oncologists in making accurate survival evaluation.

2.
Front Oncol ; 9: 1310, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31824866

RESUMO

A mutation in the isocitrate dehydrogenase 1 (IDH1) gene is the most common mutation in diffuse lower-grade gliomas (LGGs), and it is significantly related to the prognosis of LGGs. We aimed to explore the influence of the IDH1 mutation on the immune microenvironment and develop an IDH1-associated immune prognostic signature (IPS) for predicting prognosis in LGGs. IDH1 mutation status and RNA expression were investigated in two different public cohorts. To develop an IPS, LASSO Cox analysis was conducted for immune-related genes that were differentially expressed between IDH1wt and IDH1mut LGG patients. Then, we systematically analyzed the influence of the IPS on the immune microenvironment. A total of 41 immune prognostic genes were identified based on the IDH1 mutation status. A four-gene IPS was established and LGG patients were effectively stratified into low- and high-risk groups in both the training and validation sets. Stratification analysis and multivariate Cox analysis revealed that the IPS was an independent prognostic factor. We also found that high-risk LGG patients had higher levels of infiltrating B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages and dendritic cells, and expressed higher levels of CTLA-4, PD-1 and TIM-3. Moreover, a novel nomogram model was established to estimate the overall survival in LGG patients. The current study provides novel insights into the LGG immune microenvironment and potential immunotherapies. The proposed IPS is a clinically promising biomarker that can be used to classify LGG patients into subgroups with distinct outcomes and immunophenotypes, with the potential to facilitate individualized management and improve prognosis.

3.
Molecules ; 24(8)2019 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-31003503

RESUMO

Despite low fertility and content of organic carbon in albic black soil, grains are grown in this type of soil in the northeast plain of China in order to find ways to improve the soil's fertility and crop production. We carried out pot experiments of maize applied with one of three different treatments of fulvic acids (FA) derived from different parent materials: Plant-derived solid (PDSF), mineral-derived liquid (MDLF), and plant-derived liquid (PDLF) applied at respective rates of 2.5, 5, and 5 g kg-1 as well as a control applied at 0 g kg-1. The results showed that soil organic carbon and light fraction C was greater by 29% to 21% and 38% to 21%, respectively, among the treatments compared to that of the control. Similarly, available N content was significantly greater in the PDLF treatment, and P content was also significantly greater in the PDSF treatment. In contrast, available K and extractable Mg contents were lower, as well as organic-inorganic degree complexes and organic-inorganic composites in the PDSF, MDLF, and PDLF treatments compared with those of the control. Further results showed that MDLF and PDLF Fulvic acids (FA) accelerated plant growth, while PDSF limited plant growth. Our study provides empirical evidence that addition of fulvic acid from MDLF and PDLF had more positive effects on soil properties and plant growth than fulvic acid from PDSF. This investigation suggests that application of fulvic acid in liquid form can improve nutrient availability and affect other important chemical, biological, and physical properties of soils.


Assuntos
Benzopiranos/análise , Solo/química , Cálcio/análise , Carbono/análise , China , Magnésio/análise , Compostos Orgânicos/análise , Desenvolvimento Vegetal , Plantas/química
4.
Heart Vessels ; 34(6): 1052-1063, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30604190

RESUMO

It has been demonstrated that S1P receptors affect heart ischaemia-reperfusion (IR) induced injury. However, whether S1P receptors affect IR-induced cardiac death has not been investigated. The aim of this paper is to demonstrate the role of S1P receptors in IR-induced cardiac death. Healthy adult male Sprague-Dawley rats were assigned to the following groups: non-operation control group, sham operation group, IR group, IR group pretreated with DMSO, IR group pretreated with S1P3 agonist, IR group pretreated with an antagonist of S1P3, IR group pretreated with S1P2 and S1P3 antagonists, IR group pretreated with heptanol and antagonists of S1P2/3, and IR group pretreated with Gap26 and antagonists of S1P2/3 (heptanol acts as a Cx43 uncoupler and the mimic peptide Gap26 as Cx43 blocker). The groups with S1P2 or S1P3 agonist application before reperfusion were used to assess whether these can be used for therapy of IR. The haemodynamics, electrocardiograms (ECG), infarction area, and mortality rates were recorded. Immunohistological connexin 43 (Cx43) expression in the heart was detected in each group. Blocking S1P2/3 receptors with specific antagonists resulted in an increment of IR-induced mortality, increased infarction size, redistribution of Cx43 expression, as well as affecting the heart function. The infarction size, heart function, and mortality were totally or partially restored in the S1P2, S1P3 agonist-pretreated IR group, and the heptanol/Gap26-treated S1P2/3-blocked IR group. The S1P receptor S1P2/3 and Cx43 are involved in the IR-induced cardiac death.


Assuntos
Morte Súbita Cardíaca/prevenção & controle , Isquemia Miocárdica/patologia , Traumatismo por Reperfusão Miocárdica/patologia , Peptídeos/farmacologia , Receptores de Lisoesfingolipídeo/metabolismo , Animais , Conexina 43/antagonistas & inibidores , Conexina 43/metabolismo , Morte Súbita Cardíaca/etiologia , Heptanol/farmacologia , Masculino , Ratos , Ratos Sprague-Dawley , Receptores de Lisoesfingolipídeo/agonistas , Receptores de Lisoesfingolipídeo/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos
5.
World Neurosurg ; 118: e871-e879, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30031180

RESUMO

BACKGROUND: Pineoblastomas are rare, malignant embryonal tumors that have a relatively higher incidence and a poorer prognosis in children. Owing to the rarity of these tumors, there is a paucity of data on associated prognostic factors. We used the Surveillance, Epidemiology, and End Results (SEER) database to evaluate prognostic factors for pineoblastomas with the aim of improving tumor management. METHODS: Data from all pediatric patients (age ≤17 years) diagnosed with pineoblastoma between 1990 and 2013 were extracted from the SEER-18 registry database. Survival was described with Kaplan-Meier curves. The Cox proportional hazards model was used for both univariate and multivariate analyses. A nomogram was established for predicting 1-, 3-, and 5-year overall survival (OS) in patients with pineoblastoma. RESULTS: Age >5 years (P = 0.004) and radiotherapy treatment (P = 0.000) were associated with better rates of survival. Gross total resection (P = 0.054) also was correlated with better prognosis, whereas tumor size >30 mm in maximum diameter (P = 0.025) was associated with poorer outcome. A nomogram was established based on the results of the Cox model and was validated by a concordance index (C-index) of 0.767 (95% confidence interval, 0.698-0.836) and calibration plots. CONCLUSIONS: Our results show that the impact of tumor extension is not defined. OS is better in older children treated by radiotherapy, and gross total resection also appears to result in increased survival. A nomogram was built to predict 1-, 3-, and 5-year OS for these patients.


Assuntos
Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/terapia , Glândula Pineal/patologia , Pinealoma/diagnóstico , Pinealoma/terapia , Programa de SEER/tendências , Adolescente , Neoplasias Encefálicas/mortalidade , Criança , Pré-Escolar , Estudos de Coortes , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pinealoma/mortalidade , Prognóstico , Taxa de Sobrevida/tendências , Estados Unidos/epidemiologia
6.
Sci Rep ; 8(1): 2283, 2018 02 02.
Artigo em Inglês | MEDLINE | ID: mdl-29396517

RESUMO

ACS patients with renal dysfunction tend to have a poorer prognosis than those with normal renal function. This retrospective cohort study was performed using The Second Drug-Eluting Stent Impact on Revascularization Registry, a retrospective registry, to evaluate the time-dependent relative risk of revascularization strategies in ACS patients with renal dysfunction. The study demonstrated that the short-term MACCE rate was lower after PCI than CABG. However, there was no significant difference in long-term MACCE rate. Subgroup analyses based on the degree of renal dysfunction resulted in similar findings. The revascularization strategy was identified as a time-dependent covariate by the time-dependent Cox model, and the regression coefficient was '-1.124 + 0.344 × ln (time + 1)'. For the entire object group and the separate subgroups, PCI was initially associated with a lower hazard for MACCE than CABG after revascularization, then the hazard ratio increases with time. In conclusion, the hazard ratio for MACCE in PCI relative to CABG is time-dependent. PCI tends to have a lower risk for MACCE than CABG in the short-term, then the hazard ratio increases with time.


Assuntos
Síndrome Coronariana Aguda/cirurgia , Transtornos Cerebrovasculares/epidemiologia , Ponte de Artéria Coronária/efeitos adversos , Intervenção Coronária Percutânea/efeitos adversos , Insuficiência Renal/complicações , Idoso , Ponte de Artéria Coronária/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Intervenção Coronária Percutânea/métodos , Estudos Retrospectivos , Resultado do Tratamento
7.
Adv Mater ; 30(12): e1706738, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29411908

RESUMO

Recently, the quest for new highly stretchable transparent tactile sensors with large-scale integration and rapid response time continues to be a great impetus to research efforts to expand the promising applications in human-machine interactions, artificial electronic skins, and smart wearable equipment. Here, a self-powered, highly stretchable, and transparent triboelectric tactile sensor with patterned Ag-nanofiber electrodes for detecting and spatially mapping trajectory profiles is reported. The Ag-nanofiber electrodes demonstrate high transparency (>70%), low sheet resistance (1.68-11.1 Ω â–¡-1 ), excellent stretchability, and stability (>100% strain). Based on the electrode patterning and device design, an 8 × 8 triboelectric sensor matrix is fabricated, which works well under high strain owing to the effect of the electrostatic induction. Using cross-locating technology, the device can execute more rapid tactile mapping, with a response time of 70 ms. In addition, the object being detected can be made from any commonly used materials or can even be human hands, indicating that this device has widespread potential in tactile sensing and touchpad technology applications.

10.
Adv Mater ; 29(26)2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28466974

RESUMO

Pressure sensors that can both directly visualize and record applied pressure/stress are essential for e-skin and medical/health monitoring. Here, using a WO3 -film electrochromic device (ECD) array (10 × 10 pixels) and a ZnO-nanowire-matrix pressure sensor (ZPS), a pressure visualization and recording (PVR) system with a spatial resolution of 500 µm is developed. The distribution of external pressures can be recorded through the piezotronic effect from the ZPS and directly expressed by color changes in the ECD. Applying a local pressure can generate piezoelectric polarization charges at the two ends of the ZnO nanowires, which leads to the tuning of the current to be transported through the system and thus the color of the WO3 film. The coloration and bleaching process in the ECD component show good cyclic stability, and over 85% of the color contrast is maintained after 300 cycles. In this PVR system, the applied pressure can be recorded without the assistance of a computer because of the color memory effect of the WO3 material. Such systems are promising for applications in human-electronic interfaces, military applications, and smart robots.

11.
J Forensic Leg Med ; 46: 30-36, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28119211

RESUMO

Samples containing unbalanced DNA mixtures from individuals often occur in forensic DNA examination and clinical detection. Because of the PCR amplification bias, the minor contributor DNA is often masked by the major contributor DNA when using traditional STR or SNP typing techniques. Here we propose a method based in allele-specific Insertion/Deletion (INDEL) genotyping to detect DNA mixtures in forensic samples. Fourteen INDELs were surveyed in the Chinese Han population of Shanxi Province. The INDELs were amplified using two separate primer-specific reactions by real-time PCR. The difference Ct value of the 2 reactions (D-value) were used for determination of the single source DNA. INDELs types and further confirmed by electrophoresis separation. The minor allele frequency (MAF) was above 0.2 in 10 INDELs. The detection limit was 0.3125 ng-1.25 ng template DNA for real-time PCR in all 14 INDEL markers. For single source 10 ng DNA, the average D-value was 0.31 ± 0.14 for LS type, 6.96 ± 1.05 for LL type and 7.20 ± 1.09 for SS type. For the series of simulated DNA mixture, the Ct value varied between the ranges of single source DNA, depending on their INDEL typing and mixture ratios. This method can detect the specific allele of the minor DNA contributor as little as 1:50 in rs397782455 and rs397696936; 1:100 in rs397832665, rs397822382 and rs397897230; the detection limit of the minor DNA contributor was as little as 1:500-1:1000 in the rest INDEL markers, a much higher sensitivity compared with traditional STR typing. The D-value variation depended on the alternation of dilution ratio and INDEL types. When the dilution was 1:1000, the maximum and minimum D-values were 8.84 ± 0.11 in rs397897230 and 4.27 ± 0.19 in rs397897239 for LL and SS type mixture, the maximum and minimum D-values were 9.32 ± 0.54 in rs397897230 and 4.38 ± 0.26 in rs 397897239 for LL(SS) and LS type mixture, separately. Any D-value between 0.86 and 5.11 in the 14 INDELs indicated the presence of mixture. The separate amplification strategy based on real-time PCR provides a promising and convenient method for detection of unbalanced DNA mixture for Chinese Han population.


Assuntos
DNA/genética , Mutação INDEL , China , Impressões Digitais de DNA , Primers do DNA , Eletroforese , Grupos Étnicos/genética , Frequência do Gene , Genótipo , Humanos , Polimorfismo de Nucleotídeo Único , Reação em Cadeia da Polimerase em Tempo Real
12.
J Matern Fetal Neonatal Med ; 28(1): 100-5, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24588341

RESUMO

OBJECTIVE: The aim of this study is to assess the relationship between IL1RN*2 variants and preterm delivery (PTD) risk. METHODS: Eligible studies were searched in Embase and PubMed databases from inception to November 2013. Two investigators identified relevant studies and extracted data of maternal and fetal genotype independently. Based on the evidence of functional studies, we used the dominant model to all compared studies. RESULTS: To maternal genotype, 269 PTDs and 688 controls were included in meta-analysis. The overall combined odds ratio for the IL1RN*2 variant and PTD was 1.91 (95% CI, 1.41-2.58). To fetal genotype, five studies of 322 PTDs and 858 term controls were included. The result for fetal genotype analysis showed increased risk of PTD, but not significantly (OR 1.33, 95% CI 0.99-1.78). Subgroup analysis indicated that both maternal and fetal carriage of IL1RN*2 increased the risk of PTD only in studies including preterm premature rupture of membranes (PPROM), with a pooled OR 2.02 (95% CI 1.44-2.85) and 1.42 (1.02-1.99), respectively. CONCLUSIONS: This meta-analysis suggests that maternal carriage of IL1RN*2 were associated with increased risk in PTD. PPROM may be an important confounding factor that should be taken into consideration for study of IL1RN polymorphism and PTD.


Assuntos
Proteína Antagonista do Receptor de Interleucina 1/genética , Nascimento Prematuro/genética , Feminino , Ruptura Prematura de Membranas Fetais/genética , Genótipo , Humanos , Fenótipo , Polimorfismo Genético , Gravidez , Fatores de Risco
13.
Fa Yi Xue Za Zhi ; 30(4): 253-6, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25434085

RESUMO

OBJECTIVE: To test cathepsin L as a biomarker of myocardial ischemia by examination of cathepsin L expression in plasma after myocardial ischemia and ischemia-reperfusion in rats. METHODS: The rat models were established and divided in acute myocardial ischemia model (myocardial ischemia 30 min, 1 h, 2 h groups), ischemia-reperfusion model (ischemia-reperfusion group), and isoflurane-pretreated ischemia-reperfusion model (isoflurane-pretreated group), respectively. Normal control group and sham-operated group were established as contrast. The contents of cathepsin L in plasma were examined by ELISA and myocardial infarction areas were measured after TTC staining. RESULTS: No statistical significant changes were found among the experimental groups compared with the normal control group and sham-operated group (P>0.05). The cathepsin L from the ischemia-reperfusion group increased to 2.37 times compared with the normal control group (P<0.05). The cathepsin L and myocardium infarction size of isoflurane-pretreated group decreased compared with the ischemia-reperfusion group (P<0.05). CONCLUSION: The cathepsin L in plasma is not a promising biomarker of acute myocardial ischemia. Isoflurane preconditioning can reduce the cathepsin L in plasma caused by ischemia-reperfusion injury.


Assuntos
Biomarcadores/sangue , Catepsina L/análise , Infarto do Miocárdio/metabolismo , Traumatismo por Reperfusão Miocárdica/metabolismo , Animais , Isoflurano , Isquemia Miocárdica , Miocárdio , Ratos
14.
Sheng Li Xue Bao ; 66(2): 169-74, 2014 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-24777407

RESUMO

Sphingosine-1-phosphate (S1P) has been demonstrated to be a mediator and marker of heart diseases. We hypothesized that the expression of S1P receptors is involved in the S1P-mediated cardioprotection in vivo and may serve as a biomarker of ischemic heart disease. In vivo models of myocardial ischemia (MI) and ischemia-reperfusion (IR) were established by ligation of the left anterior descending artery (LAD) of rat heart, the mRNA expressions of S1PR1-3 were detected using real time PCR at different time intervals after ischemia (LAD for 15 min, 30 min, and 1 h) and IR. The results showed that mRNA expression of S1PR3, but not S1PR1 and S1PR2, increased greatly after IR. No statistical difference was found in any of the three S1P receptors after MI within 1 h. Regarding the studies of lipid concentration changes in myocardiopathy, we conclude that S1P receptors are not early response biomarkers for MI. There are different mechanisms when S1P plays a protection role in heart during MI and IR. The cooperation of lipid content and S1P receptor expression appears to form a regulation network during MI and IR.


Assuntos
Traumatismo por Reperfusão Miocárdica/fisiopatologia , Receptores de Lisoesfingolipídeo/fisiologia , Animais , Lisofosfolipídeos/fisiologia , Ratos , Esfingosina/análogos & derivados , Esfingosina/fisiologia
15.
Zhongguo Dang Dai Er Ke Za Zhi ; 16(2): 147-51, 2014 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-24568907

RESUMO

OBJECTIVE: To investigate the effects of Huangqi injection on the infection factors in children with acute lymphoblastic leukemia (ALL) during remission induction chemotherapy. METHODS: Ninety-one children with ALL were divided into treatment (n=47) and control groups (n=44) by a randomized double-blind method. During remission induction chemotherapy, the treatment group was given Huangqi injection (0.5 mL/kg·d) for 35 days, while an equal volume of normal saline was used instead in the control group; the other supportive care was the same for the two groups. After remission induction chemotherapy, the incidence of infection, duration of infection, white blood cell and neutrophil counts, site of infection, and positive rate of pathogenic bacteria in secretion were compared between the two groups. RESULTS: Four cases in the treatment group dropped out of the study due to allergic reaction. After remission induction chemotherapy, compared with the control group, the treatment group had a significantly lower incidence of infection (P<0.05), a shorter duration of infection at any site (P<0.05), a higher neutrophil count after chemotherapy (P<0.05), and lower incidence rates of respiratory tract infection, urinary tract infection, blood infection, and skin and soft tissue infections (P<0.05). Gram-negative bacteria were the main pathogens. Among the infected children, the positive rate of pathogenic bacteria in secretion was significantly lower in the treatment group than in the control group (P<0.05). CONCLUSIONS: Huangqi injection may reduce bone marrow suppression caused by chemotherapy drugs and increase neutrophil count during remission induction chemotherapy to reduce the incidence and duration of infection in children with ALL.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Adolescente , Astrágalo (Planta)/efeitos adversos , Criança , Pré-Escolar , Método Duplo-Cego , Medicamentos de Ervas Chinesas/efeitos adversos , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Humanos , Quimioterapia de Indução/efeitos adversos , Lactente , Injeções , Masculino , Neutrófilos/imunologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia
16.
IEEE Trans Inf Technol Biomed ; 11(3): 332-7, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17521083

RESUMO

We are developing an automatic captioning system for teleconsultation video teleconferencing (TC-VTC) in telemedicine, based on large vocabulary conversational speech recognition. In TC-VTC, doctors' speech contains a large number of infrequently used medical terms in spontaneous styles. Due to insufficiency of data, we adopted mixture language modeling, with models trained from several datasets of medical and nonmedical domains. This paper proposes novel modeling and estimation methods for the mixture language model (LM). Component LMs are trained from individual datasets, with class n-gram LMs trained from in-domain datasets and word n-gram LMs trained from out-of-domain datasets, and they are interpolated into a mixture LM. For class LMs, semantic categories are used for class definition on medical terms, names, and digits. The interpolation weights of a mixture LM are estimated by a greedy algorithm of forward weight adjustment (FWA). The proposed mixing of in-domain class LMs and out-of-domain word LMs, the semantic definitions of word classes, as well as the weight-estimation algorithm of FWA are effective on the TC-VTC task. As compared with using mixtures of word LMs with weights estimated by the conventional expectation-maximization algorithm, the proposed methods led to a 21% reduction of perplexity on test sets of five doctors, which translated into improvements of captioning accuracy.


Assuntos
Documentação/métodos , Linguagem , Processamento de Linguagem Natural , Consulta Remota/métodos , Interface para o Reconhecimento da Fala , Gravação em Vídeo/métodos , Videoconferência , Inteligência Artificial , Reconhecimento Automatizado de Padrão/métodos , Vocabulário Controlado
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