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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 256: 119735, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33819759

RESUMO

Hypochlorite (HClO/ClO-), an important reactive oxygen species (ROS), plays a significant role in the human immune system. Thus, developing a fast and efficient method for detecting ClO- is quite necessary. Herein, we designed and synthesized a fluorescent probe TPB-CN based on twisted intramolecular charge transfer (TICT) and aggregation-induced emission (AIE) characteristics. The probe could respond to ClO- with an ultrafast response velocity (<2 s). The detection limit was calculated to be 6.198 nM. In addition, probe TPB-CN was successfully applied for detecting ClO- in living cells and mouse.

2.
Mol Med Rep ; 23(6)2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33846776

RESUMO

Postoperative recurrence causes a high mortality rate among patients with hepatocellular carcinoma (HCC). The current study aimed to determine the effects of Plasmodium infection on HCC metastasis and recurrence. The antitumor effects of Plasmodium infection were determined using two murine orthotopic HCC models: The non­resection model and the resection model. Tumour tissues derived from tumour­bearing mice treated with or without Plasmodium infection were harvested 15 days post­tumour inoculation. The expression levels of biomarkers related to epithelial­mesenchymal transition (EMT) and molecules associated with CC­chemokine receptor 10 (CCR10)­mediated PI3K/Akt/GSK­3ß/Snail signalling were identified using reverse transcription­quantitative PCR and western blotting. The results demonstrated that Plasmodium infection significantly suppressed the progression, recurrence and metastasis of HCC in the two mouse models. The expression levels of E­cadherin were significantly higher in the Plasmodium­treated group compared with that in the control group, whereas the expression levels of Vimentin and Snail were significantly lower in the Plasmodium­treated group. Furthermore, Plasmodium infection inhibited the activation of Akt and GSK­3ß in the tumour tissues by downregulating the expression levels of CCR10 and subsequently suppressing the accumulation of Snail, which may contribute to the suppression of EMT and the prevention of tumour recurrence and metastasis. In conclusion, the results of the present study demonstrated that Plasmodium infection inhibited the recurrence and metastasis and improved the prognosis of HCC by suppressing CCR10­mediated PI3K/Akt/GSK­3ß/Snail signalling and preventing the EMT. These results may be important for the development of novel therapies for HCC recurrence and metastasis, especially for patients in the perioperative period.

3.
Spectrochim Acta A Mol Biomol Spectrosc ; 258: 119827, 2021 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-33915338

RESUMO

It is great meaningful to develop a fast and efficient method for detecting hypochlorite (ClO-) owing to its importance in the immune system. In this work, we proposed a strategy to construct fluorescent probes for ClO- based on photoinduced electron transfer (PET) mechanism. According to the strategy, we developed four fluorescent probes named TPA-NO2, TPA-2NO2, TPB-NO2 and TPB-2NO2, and studied their detecting performances for hypochlorite. Among them, TPB-NO2 displayed the most obvious fluorescence changes towards ClO- with a rapid response (<90 s). The detection limit was calculated to be 0.36 µM. Moreover, probe TPB-NO2 was successfully used to detect ClO- in living cells and zebrafish. These results demonstrated the feasibility of our strategy and provided a guidance for developing more excellent probes in the future.

4.
Chem Commun (Camb) ; 57(28): 3449-3452, 2021 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-33870345

RESUMO

Current target identification strategies mainly focus on single compounds. However, no practical experimental methodologies have been developed for multi-molecule systems. Herein, we established a cellular target identification technology for a multi-molecule system by preparing 4,4'-dihydroxybenzophenone (DHBP)-bound Fe3O4 nanoparticles (NPs) with photochemically induced crosslinking capacity. DHBP-bound NPs reacted with the chemicals from the medicinal plant extract as a multi-molecule system under ultraviolet radiation by forming carbon-carbon bonds, thus generating extract-crosslinked NPs for capturing target proteins from cell lysates. The technology, which is named the Zhao-Yao (ZY) strategy, may promote the comprehensive interpretation of the pharmacological mechanism of multi-molecule systems via the global identification of cellular targets.

5.
Mar Biotechnol (NY) ; 23(2): 255-275, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33689052

RESUMO

With atmospheric CO2 increasing, a large amount of CO2 is absorbed by oceans and lakes, which changes the carbonate system and affects the survival of aquatic plants, especially microalgae. The main aim of our study was to explore the responses of Chlamydomonas reinhardtii (Chlorophyceae) to elevated CO2 by combined transcriptome and metabolome analysis under three different scenarios: control (CK, 400 ppm), short-term elevated CO2 (ST, 1000 ppm), and long-term elevated CO2 (LT, 1000 ppm). The transcriptomic data showed moderate changes between ST and CK. However, metabolic analysis indicated that fatty acids (FAs) and partial amino acids (AAs) were increased under ST. There was a global downregulation of genes involved in photosynthesis, glycolysis, lipid metabolism, and nitrogen metabolism but increase in the TCA cycle and ß-oxidation under LT. Integrated transcriptome and metabolome analyses demonstrated that the nutritional constituents (FAs, AAs) under LT were poor compared with CK, and most genes and metabolites involved in C and N metabolism were significantly downregulated. However, the growth and photosynthesis of cells under LT increased significantly. Thus, C. reinhardtii could form a specific adaptive evolution to elevated CO2, affecting future biogeochemical cycles.

7.
Zhongguo Zhong Yao Za Zhi ; 46(3): 526-531, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33645016

RESUMO

Mechanism study was performed to explore how Shouhui Tongbian Capsules promotes energy metabolism of gastrointestinal stromal cells. In this study, gastrointestinal stromal cells line GIST-882 was used as the model to explore energy metabolism regulation effects of Shouhui Tongbian Capsules extract(10, 20, 50 and 100 µg·mL~(-1)) by measuring the cell proliferation, ATP level, mitochondrial membrane potential, and mitochondrial isocitrate dehydrogenase activity. Meanwhile, Western blot was used to detect the proteins expression of SCF/c-Kit and CDK2/cyclin A signaling pathways. Our results showed that Shouhui Tongbian Capsules promoted cell proliferation and increased ATP level of gastrointestinal stromal cells. In addition, Shouhui Tongbian Capsules obviously improved mitochondrial structural integrity, and increased mitochondrial membrane potential in GIST-882 cells. Mechanism study revealed that Shouhui Tongbian Capsules increased mitochondrial isocitrate dehydrogenase activity and up-regulated the proteins expression of SCF/c-Kit and CDK2/cyclin A signaling pathways. Collectively, our study indicated that Shouhui Tongbian Capsules promoted the energy metabolism for gastrointestinal stromal cells proliferation by activating mitochondrial isocitrate dehydrogenase to induce ATP production, as well as activating SCF/c-Kit and CDK2/cyclin A signaling pathways.


Assuntos
Tumores do Estroma Gastrointestinal , Cápsulas , Linhagem Celular Tumoral , Metabolismo Energético , Humanos , Proteínas Proto-Oncogênicas c-kit/metabolismo , Células Estromais/metabolismo
8.
Zhongguo Zhong Yao Za Zhi ; 46(4): 894-901, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33645094

RESUMO

To study the molecular mechanism of Mahuang Lianqiao Chixiaodou Decoction in the treatment of eczema by means of network pharmacology and molecular docking. First, the TCMSP database was used to excavate the active ingredient of each drug in Mahuang Lianqiao Chixiaodou Decoction and predict its target, and the Uniprot database was used to standardize the names of target proteins, in order to obtain the disease targets of eczema through GeneCards, OMIM, PharmGkb, DrugBank and other databases. And next, the potential targets on which drug targets and disease targets work together were selected to make a Venn diagram, the Cytoscape 3.6.1 software was used to screen out and construct the "active ingredient-core targets" network. STRING database was used to construct a protein-protein interaction(PPI) network, and the R language was used to perform GO enrichment analysis and KEGG pathway analysis. Finally, the molecular docking verification of main active ingredients and core targets of the drug was performed by AutoDock software. The study showed that 74 active ingredients and 103 targets of Mahuang Lianqiao Chixiaodou Decoction for the treatment of eczema were screened. The main active ingredients included quercetin, luteolin, wogonin, kaempferol, and the main targets included PTGS1, ESR1, PPARG, and MAPK3. In addition, eight key targets, including MAPK8, MAPK3, JUN, MAPK14, TP53, MAPK1, ESR1 and RELA, were calculated by PPI network. GO enrichment analysis involved 2 024 biological processes, 81 cell components, and 140 molecular functions. KEGG pathway enrichment analysis was performed to screen out 158 eczema-related pathways, which mainly acted on AGE-RAGE signaling pathway, IL-17 signaling pathway, virus-related pathways, and the results of molecular docking showed that the main active compounds could respectively bind to representative targets and exhibit a good affinity. The study proved that the treatment of eczema with Mahuang Lianqiao Chixiaodou Decoction involved multiple signaling pathways and biological processes, and the combination of main active ingredients(such as quercetin, luteolin, wogonin, kaempferol) and key targets(such as MAPK8, MAPK3, JUN, MAPK14, TP53, MAPK1, ESR1, RELA) may be one of the important mechanisms of action.


Assuntos
Medicamentos de Ervas Chinesas , Eczema , Ephedra sinica , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Simulação de Acoplamento Molecular , Tecnologia
9.
J Colloid Interface Sci ; 593: 222-230, 2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33744532

RESUMO

Surface engineering of quantum dots (QDs) plays critical roles in tailoring carriers' dynamics of I-III-VI QDs via the interplay of QDs in aggregates or assembly, thus influencing their photocatalytic activities. In this work, an aqueous synthesis and the followed pH tuned oriented assembly method are developed to prepare network-like aggregates, dispersion, or sheet-like assembly of GSH-capped Silver Indium Sulfide (AIS). FTIR, DLS, and HRTEM investigation revealed that surface protonation or deprotonation of QDs occurred at pH < 6 or pH > 12 favors the formation of network-like aggregates with various defects or sheet-like assembly with perfect crystal lattice, respectively, via the surface charge induced interaction among AIS QDs. Further UV-vis, steady and transient PL investigation confirm the narrowed band gaps and the prolonged PL lifetime of the acidic network-like aggregates. As a result, the optimized network-like aggregates (3.0-AIS) exhibits superior photocatalytic H2 evolution (PHE) rates (5.2 mmol·g-1·h-1), about 113 times that of alkaline sheet-like assembly (13.0-AIS) or 2.7 times higher than that of dispersed AIS QDs (AIS-8.0). The formation of defects and their roles in PHE mechanisms are discussed. This work is expected to give some new insight for designing efficient non-cadmium/non-novel metal I-III-VI photocatalysts for boosting PHE.

10.
Am J Rhinol Allergy ; : 1945892421993034, 2021 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-33557582

RESUMO

BACKGROUND: Recently, it has been reported that Toll-like receptor 7 (TLR7) agonists can improve allergic rhinitis (AR) symptoms by up-regulation of Th1 cytokine release and suppression of Th2 cell functions. However, little is known of the expression of TLR7 in basophils of AR. OBJECTIVE: To explore the expression of TLR7 in basophils of AR, and influence of allergens on TLR7 expression. METHODS: The expression levels of TLR7 in basophils of patients with AR were determined by flow cytometry, and the influence of allergens on TLR7 expression was examined by real time (q) PCR. RESULTS: The percentages of TLR7+CCR3+ cells (P < 0.001 and P = 0.011), TLR7+CD123+HLA-DR- cells (P = 0 .016 and P = 0.042) and TLR7+CCR3+CD123+HLA-DR- cells (P = 0.046 and P = 0.035) in blood granulocyte and mononucleated cell populations of the patients with AR were increased, respectively compared with HC subjects. TLR7 MFI on CCR3+ cells (P = 0.050 and P = 0.043), CD123+HLA-DR- cells (P < 0.001 and P = 0.002) and CCR3+CD123+HLA-DR- cells (P < 0.001 and P = 0.003) were enhanced compared with HC subjects. Allergens Der p1 and OVA provoked upregulation of TLR7 expression at both protein and mRNA levels and IL-13 production in KU812 cells. House Dust Mite extract (HDME), Artemisia sieversiana wild allergen extract (ASWE), IL-31, IL-33, IL-37, and TSLP provoked elevation of IL-6 release from KU812 cells following 2 h incubation period. CONCLUSIONS: The percentage of TLR7+ basophils and TLR7 expression intensity in a single basophil are both increased in the blood of patients with AR, indicating that basophils likely contribute to the pathogenesis of AR via TLR7.

11.
Plant Mol Biol ; 105(6): 611-623, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33528753

RESUMO

KEY MESSAGE: We applied an integrative approach using multiple methods to verify cytosine methylation in the chloroplast DNA of the multicellular brown alga Saccharina japonica. Cytosine DNA methylation is a heritable process which plays important roles in regulating development throughout the life cycle of an organism. Although methylation of nuclear DNA has been studied extensively, little is known about the state and role of DNA methylation in chloroplast genomes, especially in marine algae. Here, we have applied an integrated approach encompassing whole-genome bisulfite sequencing, methylated DNA immunoprecipitation, gene co-expression networks and photophysiological analyses to provide evidence for the role of chloroplast DNA methylation in a marine alga, the multicellular brown alga Saccharina japonica. Although the overall methylation level was relatively low in the chloroplast genome of S. japonica, gametophytes exhibited higher methylation levels than sporophytes. Gene-specific bisulfite-cloning sequencing provided additional evidence for the methylation of key photosynthetic genes. Many of them were highly expressed in sporophytes whereas genes involved in transcription, translation and biosynthesis were strongly expressed in gametophytes. Nucleus-encoded photosynthesis genes were co-expressed with their chloroplast-encoded counterparts potentially contributing to the higher photosynthetic performance in sporophytes compared to gametophytes where these co-expression networks were less pronounced. A nucleus-encoded DNA methyltransferase of the DNMT2 family is assumed to be responsible for the methylation of the chloroplast genome because it is predicted to possess a plastid transit peptide.


Assuntos
Metilação de DNA , DNA de Cloroplastos/genética , DNA de Cloroplastos/metabolismo , Feófitas/genética , Feófitas/metabolismo , Núcleo Celular , Cloroplastos/genética , Citosina/metabolismo , Regulação da Expressão Gênica de Plantas , Redes Reguladoras de Genes , Genoma de Cloroplastos , Fotossíntese
12.
Signal Transduct Target Ther ; 6(1): 71, 2021 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-33602894

RESUMO

Mitochondrial fusion/fission dynamics plays a fundamental role in neuroprotection; however, there is still a severe lack of therapeutic targets for this biological process. Here, we found that the naturally derived small molecule echinacoside (ECH) significantly promotes mitochondrial fusion progression. ECH selectively binds to the previously uncharacterized casein kinase 2 (CK2) α' subunit (CK2α') as a direct cellular target, and genetic knockdown of CK2α' abolishes ECH-mediated mitochondrial fusion. Mechanistically, ECH allosterically regulates CK2α' conformation to recruit basic transcription factor 3 (BTF3) to form a binary protein complex. Then, the CK2α'/BTF3 complex facilitates ß-catenin nuclear translocation to activate TCF/LEF transcription factors and stimulate transcription of the mitochondrial fusion gene Mfn2. Strikingly, in a mouse middle cerebral artery occlusion (MCAO) model, ECH administration was found to significantly improve cerebral injuries and behavioral deficits by enhancing Mfn2 expression in wild-type but not CK2α'+/- mice. Taken together, our findings reveal, for the first time, that CK2 is essential for promoting mitochondrial fusion in a Wnt/ß-catenin-dependent manner and suggest that pharmacologically targeting CK2 is a promising therapeutic strategy for ischemic stroke.

13.
Physiol Plant ; 2021 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-33547806

RESUMO

Oxylipins are important oxygenated derivatives of fatty acids that regulate a variety of plant physiological and pathological processes in response to specific external challenges. A large body of evidence has indicated that algae can also produce a surprisingly diverse array of volatile oxylipins, yet little is known about the roles of volatile oxylipins as defense signals in macroalgae. In this study, the kelp Macrocystis pyrifera was treated by the oxylipin messenger 1-octen-3-ol and then a genome-wide gene expression profile and fatty acid spectrum analysis were performed. We found that M. pyrifera responded rapidly to the exposure of the oxylipin messenger 1-octen-3-ol. It regulated the expression levels of genes mainly involved in signal transduction, lipid metabolism, oxidation prevention, cell wall synthesis, photosynthesis, and development. Moreover, 1-octen-3-ol treatments decreased several types of total fatty acid contents and increased free fatty acid contents, especially for the C18 and C20 fatty acids. In addition, it decreased the content of indole-3-acetic acid, abscisic acid, and zeatin and increased the gibberellic acid content. Our findings demonstrated that 1-octen-3-ol is an available inducer for M. pyrifera, which is capable of rapidly upregulating kelp's defense response.

14.
Ecotoxicol Environ Saf ; 210: 111865, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33418154

RESUMO

Cd contamination in rice grains has become a topic of great concern because of the high health risks associated with the long-term consumption of Cd-contaminated rice. Identification of Cd sources in rice grains by scientific methods is important for controlling heavy metal pollution and protecting human health. Here, the Cd concentrations and Cd isotopic compositions of rice plants (root, stem, leaf, and grain) and topsoil, and possible pollution sources (agricultural fertilizers, industrial dust, and automobile exhaust) were analyzed using an instrument of inductively coupled plasma mass spectrometry (ICP-MS) and multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS). The results showed variations in the Cd isotopes of different components of rice plants and the fractionation coefficient of rice grains relative to topsoil (Δ114/110Cdrice grains-topsoil = 0.25‰). The contributions of pollution sources to rice grains were realized by combining the Cd isotopic composition with an isotopic mixing model (Isosource). The analysis showed that all three possible pollution sources contributed to the Cd in the rice grains in the field, the average Cd contribution of industrial dust, agricultural fertilizers and automobile exhaust was 87%, 9%, and 4%, respectively. Our study provides a feasible method for the identification of pollution sources of Cd in rice grains at the field scale and demonstrates that Cd isotopic composition is one of the powerful tools to trace the pollution sources of Cd in crops.


Assuntos
Cádmio/análise , Grão Comestível/química , Oryza , Poluentes do Solo/análise , Cádmio/química , Fracionamento Químico , Poeira , Monitoramento Ambiental , Fertilizantes , Humanos , Indústrias , Isótopos/análise , Emissões de Veículos
15.
ISME J ; 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33452476

RESUMO

Arsenic pollution is a widespread threat to marine life, but the ongoing rise pCO2 levels is predicted to decrease bio-toxicity of arsenic. However, the effects of arsenic toxicity on marine primary producers under elevated pCO2 are not well characterized. Here, we studied the effects of arsenic toxicity in three globally distributed diatom species (Phaeodactylum tricornutum, Thalassiosira pseudonana, and Chaetoceros mulleri) after short-term acclimation (ST, 30 days), medium-term exposure (MT, 750 days), and long-term (LT, 1460 days) selection under ambient (400 µatm) and elevated (1000 and 2000 µatm) pCO2. We found that elevated pCO2 alleviated arsenic toxicity even after short acclimation times but the magnitude of the response decreased after mid and long-term adaptation. When fed with these elevated pCO2 selected diatoms, the scallop Patinopecten yessoensis had significantly lower arsenic content (3.26-52.83%). Transcriptomic and biochemical analysis indicated that the diatoms rapidly developed arsenic detoxification strategies, which included upregulation of transporters associated with shuttling harmful compounds out of the cell to reduce arsenic accumulation, and upregulation of proteins involved in synthesizing glutathione (GSH) to chelate intracellular arsenic to reduce arsenic toxicity. Thus, our results will expand our knowledge to fully understand the ecological risk of trace metal pollution under increasing human activity induced ocean acidification.

16.
J Investig Med ; 2020 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-33372108

RESUMO

To investigate the heterogeneity of decidual stromal cells (DSCs) and their functional alterations during delivery, we conducted single-cell RNA sequencing analysis to characterize the transcriptomic profiles of DSCs before and after labor onset. According to their transcriptomic profiles, DSCs (6382 cells) were clustered into five subgroups with different functions. Similar to stromal cells, cells in cluster 1 were involved in cell substrate adhesion. On the other hand, cells in clusters 2 and 3 were enriched in signal transduction-related genes. Labor onset led to significant alterations in many pathways, including the activator protein 1 pathway (all clusters), as well as in the response to lipopolysaccharide (clusters 1-3). The downregulated genes were involved in coagulation, ATP synthesis, and oxygen homeostasis, possibly reflecting the oxygen and energy balance during delivery. Our findings highlight that peripartum DSCs are heterogeneous and play multiple roles in labor.

17.
Scand J Immunol ; : e13003, 2020 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-33247440

RESUMO

It was reported that the expression of Toll like receptor (TLR) 9 may be related to Th2-type allergic inflammation including allergic rhinitis (AR). However, little is known about the expression of TLR9 in the basophils in AR. In the present study, the expression of TLR9 was examined by flow cytometry analysis, and the expression of TLR9 mRNA in KU812 was determined by quantitative real-time PCR. The results showed that the percentage of TLR9+ CCR3+ cells in blood granulocytes increased by 46% in patients with AR, but not in peripheral blood mononuclear cells (PBMCs). Allergens namely Dermatophagoide allergen extract (DAE) and Platanus pollen allergen extract (PPAE) upregulated the expression of TLR9 in CCR3+ granulocytes by 76% and 84%, respectively. DAE and PPAE also enhanced the proportions of TLR9+ CD123+ HLA-DR- cells and TLR9+ CCR3+ CD123+ HLA-DR- cells in granulocytes and PBMCs of patients with AR. In order to investigate the actions of allergens on basophils, KU812 cells were used. It was observed that all KU812 cells expressed TLR9, and the expression intensity of TLR9 in a single KU812 cell was elevated by CpG. IL-37, IL-31, IL-33, Artemisia sieversiana wild allergen extract (ASWAE), DAE, OVA, and Der p 1 induced an increase in the expression of TLR9 mRNA and IL-6 production in KU812 cells. It was shown that the percentage of TLR9-expressing basophils increased in the blood of ovalbumin (OVA)-sensitised mice. In conclusion, an increased expression of TLR9 and the production of IL-6 in basophils implicate that the contribution of basophils to AR is likely via TLR9.

18.
Sci Total Environ ; : 143357, 2020 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-33198997

RESUMO

Using cheap raw materials and simple biological modification methods to obtain modified materials to remediate heavy metals has potential application prospects. Here, Aspergillus niger was used to modify wollastonite and further explore its fixation capacity and mechanism. The results showed that the growth and metabolism of A. niger promoted the weathering of wollastonite and the synthesis of whewellite, forming a mineral composite through fungal modification (MCF) with excellent fixation properties of heavy metal Pb2+. The maximum adsorption capacity of MCF for Pb2+ (434.78 mg/g) is significantly greater than that of original wollastonite (11.86 mg/g) with a desired lower desorption rate. Additionally, its removal rate of Pb2+ is higher than 75% under varying acidic conditions (1 ≤ pH ≤ 5.5). The results of XRD, FTIR, BET specific surface area, and SEM-EDS showed that MCF has a special organic-inorganic composite structure, which imparts a larger contact area for ions, and efficient removal of Pb2+ through combined physico-chemical adsorption. Batch adsorption results also indicated that the adsorption process by MCF was mainly a spontaneous endothermic reaction in the monolayer. This study provides a new perspective for the bio-modification of wollastonite and its application in the remediation of heavy metal pollution.

20.
J Phycol ; 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33211355

RESUMO

Saccharina japonica is an important large brown alga and a major component of productive beds on the northwest coast of the Pacific Ocean. Abiotic stress response mechanisms are receiving considerable attention because global climate change is increasing their abiotic stress levels. However, our knowledge of how S. japonica broadly respond to stress is limited. In this study, we investigated the S. japonica responsive genes underlying acclimation to diverse stresses of acidification, high light, high temperature, hypersalinity and hyposalinity and identified 408 core genes constantly and differentially expressed in response to all stresses. Our results confrm that stresses had strong effects on genes participated in photosynthesis, amino acid metabolism, carbohydrate metabolism, halogen metabolism and reactive oxygen species defense. These findings will improve our understanding of brown algal response mechanisms linked to environmental stresses and provide a list of candidate genes for improving algal stress tolerance in light of environmental stresses in future studies.

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