Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.080
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-33847109

RESUMO

Nickel-rich Li(NixCoyMn1-x-yO2) (x ≥ 0.6) is considered to be a predominant cathode for next-generation lithium-ion batteries (LIBs) due to its towering specific energy density. Unfortunately, serious structural degradation causes rapid capacity degradation with the increase in nickel content. Herein, a Co and Ti co-modified LiNi0.8Co0.1Mn0.1O2 (NCM-811) cathode ameliorates the reversible capacity together with the rate capability by obviously alleviating the lattice structure degradation and microscopic intergranular cracks. Further studies show that the titanium doping effectively reduces the cation mixing and also stabilizes the crystal structure, while the spinel phase formed at the surface by a cobalt oxide coating is much stable than the layered phase at high voltage, which can alleviate the generation of micro-cracks. After 0.5% Co oxide coating and 1% Ti doping (T1Co0.5-NCM), a superior rate capability (121.75 mA h g-1 at 20 C between 2.7 and 4.5 V) and predominant capacity retention (74.2%) are observed compared with the pristine NCM-811 (59.5%) after 400 cycles between 2.7 and 4.7 V. This work supplies an eminent design of high-voltage and high-rate layered cathode materials and has a huge application prospect in the next generation of high-energy LIBs.

2.
Molecules ; 26(6)2021 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-33804713

RESUMO

Nutmeg is a traditional spice and medicinal plant with a variety of pharmacological activities. However, nutmeg abuse due to its hallucinogenic characteristics and poisoning cases are frequently reported. Our previous metabolomics study proved the hepatotoxicity of nutmeg and demonstrated that high-dose nutmeg can affect the synthesis and secretion of bile acids and cause oxidative stress. In order to further investigate the hepatotoxicity of nutmeg, normal saline, 1 g/kg, 4 g/kg nutmeg were administrated to male Kunming mice by intragastrical gavage for 7 days. Histopathological investigation of liver tissue, proteomics and biochemical analysis were employed to explore the mechanism of liver damage caused by nutmeg. The results showed that a high-dose (4 g/kg) of nutmeg can cause significant increased level of CYP450s and depletion of antioxidants, resulting in obvious oxidative stress damage and lipid metabolism disorders; but this change was not observed in low-dose group (1 g/kg). In addition, the increased level of malondialdehyde and decreased level of glutathione peroxidase were found after nutmeg exposure. Therefore, the present study reasonably speculates that nutmeg exposure may lead to liver injury through oxidative stress and the degree of this damage is related to the exposure dose.

3.
Hepatology ; 2021 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-33829508

RESUMO

BACKGROUND & AIMS: Due to their inherent characteristics, the function of group-2 innate lymphoid cells (ILC2s) varies in a context-dependent manner. ILC2s are involved in certain liver diseases; however, their involvement in hepatocellular carcinoma (HCC) is unknown. In the present study, we assessed the role of an HCC-derived ILC2 population in tumor progression. APPROACH & RESULTS: Through fluorescence activated cell sorting and single-cell RNA sequencing, we discovered that ILC2s were highly enriched in human HCC and correlated significantly with tumor recurrence and worse progression free as well as overall survival in patients. Mass cytometry identified a subset of HCC-derived ILC2s that had lost the expression of killer cell lectin-like receptor subfamily G, member 1 (KLRG1). Distinct from their circulating counterparts, these hepatic ILC2s highly expressed CD69 and an array of tissue resident-related genes. Furthermore, reduction of E-cadherin in tumor cells caused the loss of KLRG1 expression in ILC2s, leading to their increased proliferation and subsequent accumulation in HCC sites. The KLRG1- ILC2 subset showed elevated production of chemotaxis factors, including C-X-C motif chemokine ligand (CXCL)-2 and CXCL8, which in turn recruited neutrophils to form an immunosuppressive microenvironment, leading to tumor progression. Accordingly, restoring KLRG1 in ILC2s, inhibiting CXCL2 in ILC2s, or depleting neutrophils, inhibited tumor progression in a murine HCC model. CONCLUSION: We identified HCC-associated ILC2s as an immune regulatory cell type that promotes tumor development, suggesting that targeting these ILC2s might lead new treatments for HCC.

4.
Artigo em Inglês | MEDLINE | ID: mdl-33902333

RESUMO

Objectives: As part of a pragmatic effectiveness trial of integrative pain management among inpatients with cancer, the authors sought to understand the clinical context and adaptations to implementation of two study interventions, acupuncture and pain counseling (i.e., pain education and coping skills). Design: The larger study uses a 2 × 2 factorial design with inpatients randomized to: (1) usual care (UC), (2) UC with acupuncture, (3) UC with pain counseling, and (4) UC with acupuncture and pain counseling. The study is being conducted in two hospitals (one academic and one public) and three languages (Cantonese, English, and Spanish). The authors conducted a process evaluation by interviewing study interventionists. Analysis included deductive coding to describe context, intervention, implementation, and inductive thematic coding related to intervention delivery. Results: Interviewees included seven acupuncturists and four pain counselors. Qualitative themes covered adaptations and recognizing site-specific differences that affected implementation. Interventionists adhered closely to protocols and made patient-centered adaptations that were then standardized in broader implementation (e.g., including caregivers in pain counseling sessions; working in culturally nuanced ways with non-English-speaking patients). The public hospital included more patients with recent diagnoses and advanced disease, more ethnically and linguistically diverse patients, less continuity of staffing, and shared patient rooms. At the academic medical center, more patients were familiar with integrative therapies and all were located in single rooms. Providing acupuncture to hospital staff was a key strategy to establish trust, experientially explain the intervention, and create camaraderie and staff buy-in. Conclusions: Providing nonpharmacologic interventions for a pragmatic trial requires adapting to a range of clinical factors. Site-specific factors included greater coordination and resources needed for successful implementation in the public hospital. The authors conclude that adaptation to context and individual patient needs can be done without compromising intervention fidelity and that intervention design should apply principles such as centering at the margins to reduce participation barriers for diverse patient populations.

5.
J Ethnopharmacol ; 276: 114106, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33838287

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The Tongmai Yangxin Pill (TMYX) is a patented traditional Chinese medicine originating from two classic prescriptions, Zhigancao Decoction and Shenmai Yin, which composed of 11 Chinese medicinal herbs: Rehmannia glutinosa (Gaertn.) DC., Spatholobus suberectus Dunn, Ophiopogon japonicus (Thunb.) Ker Gawl., Glycyrrhiza uralensis Fisch., Polygonum multiflorum Thunb., Equus asinus L., Schisandra chinensis (Turcz.) Baill., Codonopsis pilosula (Franch.) Nannf., Chinemys reevesii (Gray), Ziziphus jujuba Mill. and Cinnamomum cassia (L.) J.Presl (Committee of the Pharmacopoeia of PR China, 2015). TMYX has marketed in China for the treatment of chest pain, palpitation, angina, irregular heartbeat and coronary heart disease (CHD) for several decades. Previous studies have confirmed that TMYX can treat CHD by reducing inflammation, but the underlying pharmacological mechanism remains unclear. AIM OF THE STUDY: This study aimed to declare the underlying pharmacological mechanism of anti-inflammatory activity of TMYX in the treatment of CHD via clinical trial, microarray study, bioinformatics analysis and the vitro assays. MATERIALS AND METHODS: Eight CHD patients' serum biochemical indices including coagulation function, lipid metabolism, endothelial injury, metalloprotease, adhesion molecule, inflammatory mediator and homocysteine were measured to investigate the reduction of CHD risk by TMYX oral administration (40 pills/time, 2 times/day) for eight weeks. The expression profile chips and Ingenuity Pathway Analysis (IPA) were assessed to reveal the global transcriptional response and predict related functions, diseases and canonical pathways. The in vitro anti-inflammatory actions of TMYX were evaluated using oxidized low-density lipoprotein (100 µg/mL) induced murine RAW264.7 macrophage with an ethanol extract from TMYX (EETMYX) (25-100 µg/mL). RESULTS: TMYX treatment showed reduced levels of apolipoprotein B, endothelin 1, nuclear factor κB (NF-κB) and homocysteine in CHD patients. In contrast, the treatment increased the ratio of apolipoprotein A/apolipoprotein B. EETMYX restored cell morphology and suppressed the lipid deposition of the induced foam cells. EETMYX exerted anti-inflammatory effects by raising the mRNA and protein expression of Estrogen receptor 1 (ESR1), blocking the reduction of IκBa level and the phosphorylation of IKKα/ß, IκBα and NF-κB p65, accompanied by inhibiting MCP-1, TNF-α and IL-6 production, which were consistent with bioinformatics predictions. CONCLUSION: TMYX treatment improved the biochemical indices in CHD patients. EETMYX effectively attenuated macrophage foam cell formation and exhibited anti-inflammatory activity is associated with regulating ESR1 and NF-κB signaling pathway activity.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33893114

RESUMO

To systemically understand the biosynthetic pathways of bioactive substances, including triterpenoids and polysaccharides, in Ganoderma lucidum, the correlation between substrate degradation, carbohydrate and triterpenoid metabolism during growth was analyzed by combining changes in metabolite content and changes in related enzyme expression in G. lucidum over 5 growth phases. Changes in low-polarity triterpenoid content were correlated with changes in glucose and mannitol content in fruiting bodies. Additionally, changes in medium-polarity triterpenoid content were correlated with changes in the lignocellulose content of the substrate and with the glucose, trehalose and mannitol contents of fruiting bodies. Weighted gene coexpression network analysis (WGCNA) indicated that changes in trehalose and polyol content were related to carbohydrate catabolism and polysaccharide synthesis. Changes in triterpenoid content were related to expression of the carbohydrate catabolic enzymes, laccase, cellulase, hemicellulase, and polysaccharide synthase and to the expression of several cytochrome P450 monooxygenases (CYPs). It was concluded that the products of cellulose and hemicellulose degradation participate in polyol, trehalose and polysaccharide synthesis during initial fruiting body formation. These carbohydrates accumulate in the early phase of fruiting body formation and are utilized when the fruiting bodies mature and a large number of spores are ejected. An increase in carbohydrate metabolism provides additional precursors for the synthesis of triterpenoids.ImportanceMost studies of G. lucidum have focused on its medicinal function and on the mechanism of its activity, whereas the physiological metabolism and synthesis of bioactive substances during the growth of this species have been less studied. Therefore, theoretical guidance for cultivation methods to increase the production of bioactive compounds remains lacking. This study integrated changes in the lignocellulose, carbohydrate and triterpenoid contents of G. lucidum with enzyme expression from transcriptomics data using WGCNA. The findings helped us better understand the connections between substrate utilization and the synthesis of polysaccharides and triterpenoids during the cultivation cycle of G. lucidum The results of WGCNA suggest that the synthesis of triterpenoids can be enhanced not only through regulating the expression of enzymes in the triterpenoid pathway, but also through regulating carbohydrate metabolism and substrate degradation. This study provides a potential approach and identifies enzymes that can be targeted to regulate lignocellulose degradation and accelerate the accumulation of bioactive substances by regulating substrate degradation in G. lucidum.

7.
Oncogene ; 40(17): 3136-3151, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33824473

RESUMO

Therapeutic strategies to treat pancreatic ductal adenocarcinoma (PDAC) remain unsatisfying and limited. Therefore, it is imperative to fully determine the mechanisms underlying PDAC progression. In the present study, we report a novel role of regulator of calcineurin 1, isoform 4 (RCAN1.4) in regulating PDAC progression. We demonstrated that RCAN1.4 expression was decreased significantly in PDAC tissues compared with that in para-cancerous tissues, and correlated with poor prognosis of patients with pancreatic cancer. In vitro, stable high expression of RCAN1.4 could suppress the metastasis and proliferation and angiogenesis of pancreatic tumor cells. In addition, interferon alpha inducible protein 27 (IFI27) was identified as having a functional role in RCAN1.4-mediated PDAC migration and invasion, while VEGFA play a vital role in RCAN1.4-mediated PDAC angiogenesis. Analysis of mice with subcutaneously/orthotopic implanted xenograft tumors and liver metastasis model confirmed that RCAN1.4 could modulate the growth, metastasis, and angiogenesis of tumors via IFI27/VEGFA in vivo. In conclusion, our results suggested that RCAN1.4 suppresses the growth, metastasis, and angiogenesis of PDAC, functioning partly via IFI27 and VEGFA. Importantly, our results provided possible diagnostic criteria and therapeutic targets for PDAC.

8.
Int J Obes (Lond) ; 2021 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-33907307

RESUMO

BACKGROUND/OBJECTIVES: Neck circumference, an index of upper airway fat, has been suggested to be an important measure of body-fat distribution with unique associations with health outcomes such as obstructive sleep apnea and metabolic disease. This study aims to study the genetic bases of neck circumference. METHODS: We conducted a multi-ethnic genome-wide association study of neck circumference, adjusted and unadjusted for BMI, in up to 15,090 European Ancestry (EA) and African American (AA) individuals. Because sexually dimorphic associations have been observed for anthropometric traits, we conducted both sex-combined and sex-specific analysis. RESULTS: We identified rs227724 near the Noggin (NOG) gene as a possible quantitative locus for neck circumference in men (N = 8831, P = 1.74 × 10-9) but not in women (P = 0.08). The association was replicated in men (N = 1554, P = 0.045) in an independent dataset. This locus was previously reported to be associated with human height and with self-reported snoring. We also identified rs13087058 on chromosome 3 as a suggestive locus in sex-combined analysis (N = 15090, P = 2.94 × 10-7; replication P =0.049). This locus was also associated with electrocardiogram-assessed PR interval and is a cis-expression quantitative locus for the PDZ Domain-containing ring finger 2 (PDZRN3) gene. Both NOG and PDZRN3 interact with members of transforming growth factor-beta superfamily signaling proteins. CONCLUSIONS: Our study suggests that neck circumference may have unique genetic basis independent of BMI.

9.
Dalton Trans ; 50(13): 4567-4576, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33729233

RESUMO

A hexagonal prismatic Cu6S6 cluster exhibits excellent near-infrared fluorescence properties due to its short Cu-Cu bonds, however, the construction of Cu6S6 cluster-based compounds with extended structures is still a challenge. Here, four new Cu6S6 cluster-based coordination compounds, formulated as Cu3(pymt)3 (1), {(CuCN)2[Cu3(mpymt)3]}n (2), {(CuSCN)[Cu3(mpymt)3]}n (3) and {(CuCN)2[Cu3(dmpymt)3]·CH3CN}n (4) (Hpymt = pyrimidine-2-thiolate, Hmpymt = 4-methyl-pyrimidine-2-thione and Hdmpymt = 4,6-dimethylpyrimidine-2-thione), have been synthesized through the reactions of mercaptopyrimidine derivatives and CuCN or CuSCN under solvo-thermal conditions and characterized by single-crystal X-ray diffraction, powder X-ray diffraction, IR spectroscopy, elemental analysis, and thermal gravimetric analysis. Single-crystal X-ray diffraction analysis reveals that compound 1 is a zero-dimensional Cu6(pymt)6 molecule containing a distorted pseudo-hexagonal prismatic Cu6S6 core. Compounds 2 and 4 with isomorphic frameworks but different organic linkers show a rare three-dimensional framework with nor topology constructed from Cu6(mpymt)6 units and one-dimensional chiral [Cu(CN)]n chains; compared with compound 2, a more hydrophobic one-dimensional channel in compound 4 is observed due to the increase of the methyl groups on the pyrimidine ligand, in which acetonitrile molecules are filled in the channels of compound 4. Compound 3 shows a rare two-dimensional layer constructed from Cu6(mpymt)6 units and one-dimensional puckered (CuSCN)n chains. For the first time, Cu6S6 clusters are connected to one-dimensional inorganic CuCN (or CuSCN) chains through mercaptopyrimidine derivatives to obtain extended arrays in compounds 2-4. The crystals of compounds 1-4 in the solid state all show apparent red light emission. Compound 4 shows sensitive luminescence quenching response to nitrobenzene (NB), and the corresponding quenching constant (Ksv) and detection limit are 2.06 × 103 M-1 and 9.27 ppm, respectively. This study provides a new strategy to construct Cu6S6 cluster-based coordination polymers that have great potential in various applications such as luminescence sensing.

10.
World Neurosurg ; 2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33737256

RESUMO

OBJECTIVE: Facial nerve (FN) function preservation is the primary goal during vestibular schwannoma (VS) resection. Many factors are linked to postoperative FN outcomes. In the present study, we evaluated the association between FN length and VS surgical outcomes. METHODS: We included 70 consecutive patients who had undergone VS microsurgery between October 2019 and November 2020. The clinical data were prospectively obtained from the patients. The relative FN (rFN) length was obtained by subtracting the contralateral FN length from the ipsilateral FN length as measured using DSI Studio software (available at: http://dsi-studio.labsolver.org/). RESULTS: The postoperative FN function was House-Brackmann grade I in 47 of the 70 patients (67.1%), grade II in 10 (14.3%), and grade III in 13 (18.6%). Gross total resection (GTR) was performed in 61 patients (87.1%). A residual tumor was retained to preserve FN function in 9 of the 70 patients (12.9%), and rFN length was measured (mean diameter, 20.8 mm; range, 2.5-51.5]). On multivariate analysis, the rFN length was significantly associated with the extent of tumor resection. The receiver operating characteristic curve indicated that the cutoff value for rFN length to predict for intraoperative near total resection versus GTR was 36.6 mm, with a specificity and sensitivity of 93.4% and 88.9%, respectively. CONCLUSIONS: The rFN length is important for predicting surgical outcomes. An rFN length >36.6 mm might indicate difficulty in achieving GTR with preservation of FN function. Therefore, the rFN length could become an objective indicator for neurosurgeons to predict the difficulty of GTR to preserve FN function.

11.
Nat Commun ; 12(1): 1755, 2021 03 19.
Artigo em Inglês | MEDLINE | ID: mdl-33741935

RESUMO

All-electronic interrogation of biofluid flow velocity by electrical nanosensors incorporated in ultra-low-power or self-sustained systems offers the promise of enabling multifarious emerging research and applications. However, existing nano-based electrical flow sensing technologies remain lacking in precision and stability and are typically only applicable to simple aqueous solutions or liquid/gas dual-phase mixtures, making them unsuitable for monitoring low-flow (~micrometer/second) yet important characteristics of continuous biofluids (such as hemorheological behaviors in microcirculation). Here, we show that monolayer-graphene single microelectrodes harvesting charge from continuous aqueous flow provide an effective flow sensing strategy that delivers key performance metrics orders of magnitude higher than other electrical approaches. In particular, over six-months stability and sub-micrometer/second resolution in real-time quantification of whole-blood flows with multiscale amplitude-temporal characteristics are obtained in a microfluidic chip.


Assuntos
Grafite/química , Microeletrodos , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Animais , Velocidade do Fluxo Sanguíneo , Bovinos , Cobre/química , Dispositivos Lab-On-A-Chip , Fenômenos Físicos , Polimetil Metacrilato/química
12.
Nat Commun ; 12(1): 1758, 2021 03 19.
Artigo em Inglês | MEDLINE | ID: mdl-33741948

RESUMO

The molecular machinery and chromosome structures carrying out meiosis are frequently conserved from yeast to mammals. However, signals initiating meiosis appear divergent: while nutrient restriction induces meiosis in the yeast system, retinoic acid (RA) and its target Stra8 have been shown to be necessary but not sufficient to induce meiotic initiation in mammalian germ cells. Here, we use primary culture of mouse undifferentiated spermatogonia without the support of gonadal somatic cells to show that nutrient restriction in combination with RA is sufficient to induce Stra8- and Spo11-dependent meiotic gene and chromosome programs that recapitulate the transcriptomic and cytologic features of in vivo meiosis. We demonstrate that neither nutrient restriction nor RA alone exerts these effects. Moreover, we identify a distinctive network of 11 nutrient restriction-upregulated transcription factor genes, which are associated with early meiosis in vivo and whose expression does not require RA. Our study proposes a conserved model, in which nutrient restriction induces meiotic initiation by upregulating key transcription factor genes for the meiotic gene program and provides an in vitro platform for meiotic induction that could facilitate research and haploid gamete production.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Endodesoxirribonucleases/metabolismo , Meiose/efeitos dos fármacos , Nutrientes/metabolismo , Espermatogônias/efeitos dos fármacos , Tretinoína/farmacologia , Proteínas Adaptadoras de Transdução de Sinal/genética , Animais , Células Cultivadas , Endodesoxirribonucleases/genética , Expressão Gênica/efeitos dos fármacos , Masculino , Mamíferos/genética , Mamíferos/metabolismo , Meiose/genética , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Camundongos Knockout , Análise de Sequência de RNA/métodos , Análise de Célula Única/métodos , Espermatogônias/citologia , Espermatogônias/metabolismo
13.
Cell Death Dis ; 12(4): 328, 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33771982

RESUMO

Metabolic reprogramming is a new hallmark of cancer but it remains poorly defined in hepatocellular carcinogenesis (HCC). The fatty acid receptor CD36 is associated with both lipid and glucose metabolism in the liver. However, the role of CD36 in metabolic reprogramming in the progression of HCC still remains to be elucidated. In the present study, we found that CD36 is highly expressed in human HCC as compared with non-tumor hepatic tissue. CD36 overexpression promoted the proliferation, migration, invasion, and in vivo tumor growth of HCC cells, whereas silencing CD36 had the opposite effects. By analysis of cell metabolic phenotype, CD36 expression showed a positive association with extracellular acidification rate, a measure of glycolysis, instead of oxygen consumption rate. Further experiments verified that overexpression of CD36 resulted in increased glycolysis flux and lactic acid production. Mechanistically, CD36 induced mTOR-mediated oncogenic glycolysis via activation of Src/PI3K/AKT signaling axis. Pretreatment of HCC cells with PI3K/AKT/mTOR inhibitors largely blocked the tumor-promoting effect of CD36. Our findings suggest that CD36 exerts a stimulatory effect on HCC growth and metastasis, through mediating aerobic glycolysis by the Src/PI3K/AKT/mTOR signaling pathway.

14.
J Hypertens ; 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33783375

RESUMO

OBJECTIVE: Pregnancy-associated plasma protein-A2 (PAPP-A2) is the homolog of PAPP-A in the vertebrate genome and its role in protecting against salt-induced hypertension in salt-sensitive rats has been confirmed. We sought to examine the associations of plasma PAPP-A2 levels and its genetic variants with salt sensitivity, blood pressure (BP) changes and hypertension incidence in humans. METHODS: Eighty participants (18-65 years old) sequentially consuming a usual diet, a 7-day low-salt diet (3.0 g/day) and a 7-day high-salt diet (18 g/day). In addition, we studied participants of the original Baoji Salt-Sensitive Study, recruited from 124 families in Northern China in 2004 who received the same salt intake intervention, and evaluated them for the development of hypertension over 14 years. RESULTS: The plasma PAPPA2 levels significantly decreased with the change from baseline to a low-salt diet and decreased further when converting from the low-salt to high-salt diet. SNP rs12042763 in the PAPP-A2 gene was significantly associated with systolic BP responses to both low-salt and high-salt diet while SNP rs2861813 showed a significant association with the changes in SBP and pulse pressure at 14-year follow-up. Additionally, SNPs rs2294654 and rs718067 demonstrated a significant association with the incidence of hypertension over the 14-year follow-up. Finally, the gene-based analysis found that Pappa2 was significantly associated with longitudinal SBP changes and the incidence of hypertension over the 14-year follow-up. CONCLUSIONS: This study shows that dietary salt intake affects plasma PAPP-A2 levels and that PAPP-A2 may play a role in salt sensitivity, BP progression and development of hypertension in the Chinese populations.

15.
Aesthetic Plast Surg ; 2021 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-33758977

RESUMO

BACKGROUND: Intraoperative symmetrical assessment plays a decisive role in the aesthetic results of reduction mammaplasty, but it depends mostly on the surgeons' experience that may be biased by individual subjective factors. This study was intended to propose an objective method based on a hand-held three-dimensional (3D) scanner to assist intraoperative symmetrical assessment, aiming to achieve better aesthetic results in reduction mammaplasty. METHODS: Sixty patients were enrolled in the study from April 2018 to January 2020. Intraoperative 3D scanning was routinely performed on 29 patients (study group) to assist symmetrical adjustments during breast shaping. 3D surface scanning data of both groups were obtained at 3 months postoperatively to objectively assess breast symmetry. Postoperative symmetry scores in five aspects, including nipple-areolar complex position, inframammary-fold height, breast size, shape, and footprint, were rated by six independent observers based on anonymized photographs to subjectively evaluate pre- and postoperative breast symmetry of the two groups. RESULTS: The bilateral breast volume difference of the study group was significantly smaller than the control group (39.1 vs. 113.3 cm3, p = 0.001), as well as the difference in nipple to inframammary-fold distance (2.79 vs. 7.43 mm, p = 0.01). The observer-reported results showed that breast reduction significantly improved postoperative symmetry in all five aspects compared with preoperative symmetry in the study group (P<0.001). Furthermore, postoperative symmetrical ratings of all five aspects in the study group were statistically better than the control group (P<0.05). CONCLUSIONS: Intraoperative 3D scanning provided a reliable method to assist symmetry adjustments and ensure better postoperative breast symmetry in reduction mammaplasty. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

16.
Small ; 17(12): e2006723, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33656258

RESUMO

The diversity by nano/microstructural material or device constructing can provide the exciting opportunity for sensitivity and selectivity to achieve facile and efficient multianalyte recognition for clinical diagnosis, environment monitoring, etc., in complex system analysis. Colloidal poly(styrene-methyl methacrylate-acrylic acid) (poly(St-MMA-AA)) nanoparticle-assembled photonic crystals (PCs) can achieve manipulative 3D structural colors and approach PC sensor chip for high-efficient multianalysis utilizing simple dye. Focusing on the morphology effects of structural color, a PC microchip is designed and constructed with various geometrical micromorphologies. Based on the angle dependence of colloidal-crystal structural color, the stopband distribution is explored on various morphological PC pixels. Selective fluorescent enhancement is realized for stopband-matched PCs, which approach the successful discrimination of metal ions and complex multianalysis of groundwater. Meanwhile, printed droplet-shaping manipulation can achieve a large-scale structural-color sensor array of chips with designable nano/microstructures via colloidal assembly. It will be the critical puzzle piece between macromorphology and microstructure for the structural-color researches.

17.
Brain Struct Funct ; 226(4): 951-961, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33710409

RESUMO

The impressive functions of the brain rely on an extensive connectivity matrix between specific neurons, the architecture of which is frequently characterized by one brain nucleus/region connecting to multiple targets, either via collaterals of the same projection neuron or several, differentially specified neurons. Delineating the fine architecture of projection neuron subsets in a specific brain region could greatly facilitate its circuit, computational, and functional resolution. Here, we developed multiple fluorescent rabies viruses (RV) to delineate the fine organization of corticothalamic projection neuron subsets in the primary visual cortex (V1). By simultaneously retrograde labeling multiple distinct subsets of corticothalamic projection neurons in V1 from their target nuclei in thalamus (dLGN, LP, LD), we observed that V1-dLGN corticothalamic projection neurons were densely concentrated in layer VI, except for several sparsely scattered neurons in layer V, while V1-LP and V1-LD corticothalamic projection neurons were localized to both layers V and VI. Meanwhile, we observed a fraction of V1 corticothalamic projection neurons targeting two thalamic nuclei, which was further confirmed by fMOST whole-brain imaging. The multiple fluorescent RV tracing tools can be extensively applied to resolve the architecture of projection neuron subsets in certain brain regions, with a strong potential to delineate the computational and functional organization of these brain regions.

18.
Sci Total Environ ; 778: 146245, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33711589

RESUMO

Condensable particulate matter (CPM) is a special kind of primary particulate matter and is in a gaseous state before discharge. After discharge, it rapidly forms liquid or solid particles through atmospheric dilution and cooling, which are harmful to the environment and human health. However, current research on controlling CPM is lacking. Therefore, the adsorption effects of activated carbons (ACs) on CPM at different temperatures were studied using EPA Method 202. Results showed that the removal efficiency range of CPM at 90 °C by ACs could reach 19%-22%. The removal efficiency of the inorganic fraction was higher than that of the organic fraction. ACs had obvious adsorption effects on Cl-, NH4+, and Hg in CPM but had marginal adsorption effects on SO42+, NO3-, and other metal elements in CPM. ACs had prominent adsorption effects on extremely toxic aromatic compounds in CPM. At a flue gas temperature of 35-170 °C, the efficiency of CPM removal through AC adsorption could increase with decreasing flue gas temperature, and this effect was more obvious during the adsorption of inorganic fractions. In addition, the efficiency of CPM removal through condensation and adsorption could reach up to 51% at 35 °C when flue gas at 130 °C was used as the initial flue gas.

19.
Zhongguo Gu Shang ; 34(1): 40-4, 2021 Jan 25.
Artigo em Chinês | MEDLINE | ID: mdl-33666018

RESUMO

OBJECTIVE: To investigate the biomechanical affect of percutaneous transforaminal endoscopic discectomy(PTED) on adjacent segments with different degrees of degeneration and related risk of adjacent segment diseases (ASD) caused by this operation. METHODS: A healthy male adult volunteer was selected, and the lumbosacral vertebra image data was obtained by CT scan, and the external contour of the bone structure was reconstructed. On this basis, the external contour of the bone structure was fitted by using the smooth curve in 3D-CAD software, and the complete three-dimensional finite element modelof the non degenerate L3-S1 segment and the degenerative models of the L3-L4 and L5-S1 segment were drawn forward. In L4, L5 segment simulating PTED surgery through the removal of right part of articular process and nucleus pulposus and anulus fibrosus. After PTED was simulated in the L4-L5 segment and the risk of ASD has been evaluated by six changes of biomechanical indicators in flexion, extension, left and right lateral bending, left and right axial rotation conditions. RESULTS: In the finite element model without adjacent segmental disc degeneration, the annulus fibrosus von Mises stress and intradiscal pressure of the PTED model showed only a slight increase under most stress conditions, and a slight decrease in a few conditions, and there was no significant change trend before and after surgery. In the original degenerated adjacent segment disc model, the biomechanical indicators related to disc degeneration in the pre- and post-PTED model showed significant deterioration, leading to an increased risk of potential adjacent spondylopathy. CONCLUSION: PTED surgery will not lead to the significant deterioration of postoperative biomechanical environment of non-degeneration adjacent intervertebral discs, and the original degeneration of adjacent intervertebral discs is a important risk factor for ASD.


Assuntos
Discotomia Percutânea , Degeneração do Disco Intervertebral , Disco Intervertebral , Adulto , Fenômenos Biomecânicos , Análise de Elementos Finitos , Humanos , Disco Intervertebral/cirurgia , Degeneração do Disco Intervertebral/cirurgia , Vértebras Lombares/cirurgia , Masculino , Amplitude de Movimento Articular
20.
ChemSusChem ; 14(8): 1847-1852, 2021 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-33733591

RESUMO

Electrochemical conversion of CO2 into value-added fuels and feedstocks attracts worldwide attention to mitigate energy and environmental problems. However, pursuing highly efficient electrocatalyst is still a challenge. In this study, cuprous oxide (Cu2 O) modified by cucurbit[6]urils (Q[6]), a kind of rigid macromolecule, is found to act as an efficient supramolecular inorganic nanocomposite catalyst for the electrochemical CO2 reduction reaction (CO2 RR) to C1 fuels. This catalyst affords a high total faradaic efficiency (FECO+formate ) of 93.96 % at a potential of -0.7 V vs. reversible hydrogen electrode and over 85 % from -0.6 to -0.9 V in 0.5 M KHCO3 , which is higher than that of pure Cu2 O (39.89 %). The enhancements in selectivity and activity for CO2 RR could significantly benefit from the strong CO2 adsorption capacity and hydrophobic nature of the cavity of Q[6], which simultaneously trap gaseous reactants near the catalyst to tune the local environment and limit the diffusion of water molecules. This study provides a strategy to adjust catalytic environments through supramolecular engineering.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...