Clay-based aerogel combined with CuS for solar-driven interfacial steam generation and desalination.

Solar-driven water purification is a promising technology that can effectively utilize solar energy for seawater desalination. However, poor materials are unable to meet the dense energy of solar steam generation in natural sunlight for real-time practical applications. Therefore, the demand for energy density can be increased by using improved semiconductor aerogel materials. Here, we report a simple chemical method to obtain a CuS@ATP/PS composite aerogel (named CuAP), which was made of attapulgite (ATP) and CuS loaded onto it using an N-template to give it good photothermal characteristics (CuS@ATP), and then cross-link it with potato starch (PS). The evaporation rate of CuAP-15 aerogel in pure water at 1 kW m-2 solar radiation is 1.57 kg m-2 h-1. Meanwhile, CuAP-15 aerogel showed excellent salt resistance with an evaporation rate of 1.35 kg m-2 h-1 in 20 wt% NaCl solution. And also exhibited excellent cycling durability in cycling stability tests. More importantly, the freshwater yield can reach 6.54 kg m-2 under natural light irradiation for 11 h. Therefore, CuAP aerogel has a great prospect of application in the field of seawater desalination in the future.

Smart antioxidant function enhancing (SAFE) nucleic acid therapy for ROS-related chronic diseases and comorbidities.

Reactive oxygen species (ROS)-mediated oxidative stress exacerbates chronic diseases such as organ damage and neurodegenerative disorders. The Keap1-Nrf2-ARE pathway is a widely distributed endogenous antioxidant system. However, ROS under redox homeostasis regulates a wide range of life activities. Therefore, smart scavenging of excess ROS under pathological conditions is essential to treat chronic diseases safely. This study reports a smart antioxidant function enhancement (SAFE) strategy. On-demand release of nucleic acid drugs in a pathological ROS environment smartly activates the endogenous antioxidant system, thereby smartly alleviating oxidative stress in an exogenous antioxidant-independent manner. Through structural modulation and ligand modification, we develop SAFE nanoparticles based on nanohybrid complexes (SAFE-complex) adapted to brain delivery of nucleic acid drugs. SAFE-complex with homogeneous monodisperse structure efficiently treat ROS-related neurodegenerative diseases while protecting the major organ from oxidative stress damage. Moreover, SAFE-complex can stabilize storage in the form of freeze-dried powder. These data indicate that SAFE nanoparticles hold promise for treating ROS-related chronic diseases and comorbidities through rational transformation.

Qualitative and quantitative studies of phthalates in extra virgin olive oil (EVOO) by surface-enhanced Raman spectroscopy (SERS) combined with long short term memory (LSTM) neural network.

Phthalates are commonly used plasticizers in the plastics industry, and have received extensive attention due to their reproductive toxicity. Since phthalates are lipophilic solutions, phthalates can easily migrate from packaging to edible oils. This study synthesized stable and sensitive Gold Nanostars as SERS substrates to conduct qualitative and quantitative analysis of two common phthalates, dibutyl phthalate and di(2-ethylhexyl) phthalate. Two ethanol standard solutions and actual oil solutions of phthalates at different concentrations (10, 5, 1, 0.5, 0.1, 0.02 mg/kg) were prepared. After dimension reduction, LSTM achieved the accuracy of 98% for pure EVOO and EVOO adulterated with different types of phthalates. In terms of quantification, LSTM demonstrates great predictive performance with Rp2 greater than 0.97 and the ratio of performance to deviation greater than 5. These results have certain guiding significance for the analysis of plasticizers in edible oil.

Full-thickness osteochondral defect repair using a biodegradable bilayered scaffold of porous zinc and chondroitin sulfate hydrogel.

The regeneration of osteochondral tissue necessitates the re-establishment of a gradient owing to the unique characteristics and healing potential of the chondral and osseous phases. As the self-healing capacity of hyaline cartilage is limited, timely mechanical support during neo-cartilage formation is crucial to achieving optimal repair efficacy. In this study, we devised a biodegradable bilayered scaffold, comprising chondroitin sulfate (CS) hydrogel to regenerate chondral tissue and a porous pure zinc (Zn) scaffold for regeneration of the underlying bone as mechanical support for the cartilage layer. The photocured CS hydrogel possessed a compressive strength of 82 kPa, while the porous pure Zn scaffold exhibited a yield strength of 11 MPa and a stiffness of 0.8 GPa. Such mechanical properties are similar to values reported for cancellous bone. In vitro biological experiments demonstrated that the bilayered scaffold displayed favorable cytocompatibility and promoted chondrogenic and osteogenic differentiation of bone marrow stem cells. Upon implantation, the scaffold facilitated the simultaneous regeneration of bone and cartilage tissue in a porcine model, resulting in (i) a smoother cartilage surface, (ii) more hyaline-like cartilage, and (iii) a superior integration into the adjacent host tissue. Our bilayered scaffold exhibits significant potential for clinical application in osteochondral regeneration.

Comparison of Raman spectral characteristics and quantitative methods between 13CH4 and 12CH4 from 25 to 400 °C and 50 to 400 bar.

In recent years, increasing attention has been given to quantifying the isotopic compositions of gases by Raman spectroscopy. However, related research on the carbon isotopes of CH4 is still lacking. In this study, the Raman spectral characteristics of 13CH4 and 12CH4 in the pure CH4 system and in the CH4-H2O system are comprehensively studied at temperatures ranging from 25 to 400 °C and pressures ranging from 50 to 400 bar. For the pure CH4 system, the peak positions of the symmetric stretching band (ν1) of 13CH4 and 12CH4 both shift to a higher wavenumber with increasing temperature and decreasing pressure, and their full width at half maximum (FWHM) values can reach 4.7 cm-1 and 5.5 cm-1, respectively, with increasing temperature and density. The peak position of the ν1 band of 13CH4 is always 4.6-5.1 cm-1 lower than that of 12CH4 under the same conditions, indicating that 13CH4 and 12CH4 can be distinguished by peak position and FWHM in the Raman spectra. Although the calculated Raman shifts (ν0) at zero-density for 13CH4 and 12CH4 are different, the unified equation for determining the density of CH4-rich fluid inclusions is still applicable, as long as to bracket the sample measurement with measurements of reference standards. For the CH4-H2O system, the peak position of the ν1 band of dissolved 13CH4 is approximately 2.5 cm-1 lower than that of 12CH4, while their FWHM values can be more than 12.2 cm-1, causing more difficulty in distinguishing them in aqueous solutions. In addition, Raman quantitative calibration models of 12CH4 and 13CH4 in the CH4-H2O system are established. The difference in the Raman scattering cross section (RSCS) of dissolved 12CH4 and 13CH4, rather than changes in the molar density or RSCS of water is the main reason leading to the difference in the Raman quantitative calibration models of CH4 concentrations between the 12CH4-H2O system and the 13CH4-H2O system. Our work provides a good reference for the in situ identification and quantification of the carbon isotopic composition of CH4 in high-temperature and high-pressure environments.

Structural, extraction and safety aspects of novel alternative proteins from different sources.

With rapid population growth and continued environmental degradation, it is no longer sustainable to rely on conventional proteins to meet human requirements. This has prompted the search for novel alternative protein sources of greater sustainability. Currently, proteins of non-conventional origin have been developed, with such alternative protein sources including plants, insects, algae, and even bacteria and fungi. Most of these protein sources have a high protein content, along with a balanced amino acid composition, and are regarded as healthy and nutritious sources of protein. While these novel alternative proteins have excellent nutritional, research on their structure are still at a preliminary stage, particularly so for insects, algae, bacteria, and fungi. Therefore, this review provides a comprehensive overview of promising novel alternative proteins developed in recent years with a focus on their nutrition, sustainability, classification, and structure. In addition, methods of extraction and potential safety factors for these proteins are summarized.

A highly stretchable, adhesive, and antibacterial hydrogel with chitosan and tobramycin as dynamic cross-linkers for treating the infected diabetic wound.

Diabetic wounds pose a significant challenge due to their susceptibility to bacterial infection in a high-glucose environment, which impedes the wound healing process. To address this issue, there is a pressing need to develop suitable hydrogels that can promote the regeneration of diabetic wounds in clinical practice. In this study, we designed and fabricated a highly stretchable, adhesive, transparent, and antibacterial hydrogel through a one-pot radical polymerization of N-[Tris (hydroxymethyl) methyl] acrylamide (THMA) and acrylic acid (AA), and with chitosan and the antibiotic tobramycin as the dynamic physical crosslinkers. The copolymer contains a large number of carboxyl and hydroxyl groups, which can form an interpenetrating network structure with chitosan and tobramycin through multiple dynamic non-covalent bonds. This hydrogel exhibited over 1600 % elongation through an energy dissipation mechanism and strong adhesion to various surfaces without any chemical reaction. In vivo, studies conducted on a staphylococcus aureus-infected full-thickness diabetic skin wound model demonstrated that the hydrogel loaded with tobramycin as one of the crosslinkers had a long-lasting antibacterial activity and effectively accelerated wound healing. Therefore, the antibiotic-loaded adhesive hydrogel we proposed holds great promise as a treatment for bacteria-infected diabetic wounds.

Carbon dots/Ruthenium(III) nanocomposites for FRET fluorescence detection and removal of mercury (II) via assembling into nanofibers.

The determination and removal of mercury(II) (Hg2+) are essential for human health and environmental ecosystems. Herein, an ingenious carbon dots (CDs)-based Förster resonance energy transfer (FRET) system (N, S-CDs/Ru) was fabricated employing CDs and Ru3+ units as energy-transfer doner/acceptor pairs for visual detection and efficient removal of Hg2+. The treatment of Hg2+ induced a remarkable linear enhancement of the ratiometric fluorescence (F613 nm/F478 nm) with a detection limit (LOD) of 95 nM, along with continuous fluorescence color variations from blue to red. Given that the fluorescence color recognition and processing realized the real-time and rapid quantitation of Hg2+ by paper-based smartphone sensing platform. The mechanistic study revealed that the N/S/O-rich surface of the system enabled the Hg2+-triggered self-assembly from dots to nanofibers, combing with the active FRET process. Also, the efficient removal of Hg2+ with a removal efficiency of ∼98 % and an adsorption capacity of ∼372 mg/g was obtained. Furthermore, it was found that N, S-CDs/Ru loaded commercialized SiO2 or SBA-15 could facilitate the removal of Hg2+ with a removal efficiency over 99 % and an adsorption capacity up to ∼562 mg/g. This study provides a potential strategy for environmental monitoring and remediation.

Drivers and impacts of decreasing concentrations of atmospheric volatile organic compounds (VOCs) in Beijing during 2016-2020.

China has implemented various policies and measures for controlling air pollutants. However, our knowledge of the long-term trends in ambient volatile organic compounds (VOCs) after the implementation of these action plans in China remains limited. To address this, we conducted a five-year analysis (2016-2020) of VOC compositions and concentrations in Beijing. The annual VOC concentration decreased from 44.0 ± 28.8 to 26.2 ± 16.4 ppbv, with alkanes being the most prevalent group. The annual average concentrations of alkenes, alkynes, and aromatics have experienced a significant decrease of over 50 %. Seasonal variations indicated higher VOC concentrations in winter and autumn, with more significant reductions observed in winter and autumn. The impact of meteorological conditions caused variations in VOC reductions during the Chinese Spring Festival. Satellite-based measurements of formaldehyde (HCHO) columns confirmed the reduction of VOC emissions during the Coronavirus (COVID-19) lockdown. The normalized annual average VOC concentration decreased by 2.9ppbv yr-1 from 2016 to 2020, and emission reduction contributed to 58.8 % of VOC reduction from 2016 to 2020 after meteorological normalization, indicating the effectiveness of implemented control measures. Based on receptor model, vehicle emissions and industrial sources were identified as the largest contributors to VOC concentrations. Vehicle emissions, liquefied petroleum gas/natural gas (LPG/NG) use, and coal combustion were major drivers of VOC reduction. Potential source region analysis revealed that air masses transported from northwestern and southern regions significantly contributed to VOC concentrations in Beijing. The range of source regions shrunk in both northwestern and southern regions with the reduction in VOC concentrations. The annual variations of ozone formation potential indicated a significant decrease in VOC reactivities through emission control. These results could provide insights into future emission control and coordinated efforts to improve PM2.5 and ozone levels in China.

Raman spectroscopy combined with multiple one-dimensional deep learning models for simultaneous quantification of multiple components in blended olive oil.

Blended vegetable oils are highly prized by consumers for their comprehensive nutritional profile. Therefore, there is an urgent need for a rapid and accurate method to identify the true content of blended oils. This study combined Raman spectroscopy with three deep learning models (CNN-LSTM, improved AlexNet, and ResNet) to simultaneously quantify extra virgin olive oil (EVOO), soybean oil, and sunflower oil in olive blended oil. The results demonstrate that all three deep learning models exhibited superior predictive ability compared to traditional chemometric methods. Specifically, the CNN-LSTM model achieved a coefficient of determination (R2p) of over 0.995 for each oil in the quantitative analysis of three-component blended oils, with a mean square error of prediction (RMSEP) of less than 2%. This study presents a novel approach for the simultaneous quantitative analysis of multi-component blended oils, providing a rapid and accurate method for the identification of falsely labeled blended oils.

The 2022 report of synergetic roadmap on carbon neutrality and clean air for China: Accelerating transition in key sectors.

China is now confronting the intertwined challenges of air pollution and climate change. Given the high synergies between air pollution abatement and climate change mitigation, the Chinese government is actively promoting synergetic control of these two issues. The Synergetic Roadmap project was launched in 2021 to track and analyze the progress of synergetic control in China by developing and monitoring key indicators. The Synergetic Roadmap 2022 report is the first annual update, featuring 20 indicators across five aspects: synergetic governance system and practices, progress in structural transition, air pollution and associated weather-climate interactions, sources, sinks, and mitigation pathway of atmospheric composition, and health impacts and benefits of coordinated control. Compared to the comprehensive review presented in the 2021 report, the Synergetic Roadmap 2022 report places particular emphasis on progress in 2021 with highlights on actions in key sectors and the relevant milestones. These milestones include the proportion of non-fossil power generation capacity surpassing coal-fired capacity for the first time, a decline in the production of crude steel and cement after years of growth, and the surging penetration of electric vehicles. Additionally, in 2022, China issued the first national policy that synergizes abatements of pollution and carbon emissions, marking a new era for China's pollution-carbon co-control. These changes highlight China's efforts to reshape its energy, economic, and transportation structures to meet the demand for synergetic control and sustainable development. Consequently, the country has witnessed a slowdown in carbon emission growth, improved air quality, and increased health benefits in recent years.

Calcium-based biomaterials: Unveiling features and expanding applications in osteosarcoma treatment.

Calcium, an indispensable element in bone tissues, plays a crucial role in various cellular processes involved in cancer progression. Its ubiquitous yet spatially distinct distribution in the body presents an opportunity to target calcium homeostasis as a novel strategies for cancer treatment, with specific advantages in osteosarcoma therapy. In this comprehensive review, we retrospect the calcium biology intersected with cancer progression, highlight the unveiling features of calcium-based biomaterials in regulating both bone homeostasis and cancer development. We also provide an overview of recent breakthroughs in cancer therapy that leverage calcium biomaterials, showcasing their potential to serve as versatile, customizable platforms for osteosarcoma treatment and as reservoirs for supporting bone reconstruction.

Biosynthesis of gallic acid fabricated tellurium nanoparticles (GA-Te NPs) for enhanced antibacterial, antioxidant, and cytotoxicity applications.

The development of antibiotic resistance and the onset of diverse forms of cancer necessitate the utilization of innovative multifunctional biocompatible materials. The synthesis of metal and metalloid nanoparticles through eco-friendly means demonstrates promising potential in therapeutic and diagnostic domains. Among these materials, Tellurium (Te) exhibits exceptional characteristics and finds application in numerous fields; nevertheless, its usage in biological applications has been somewhat limited, primarily due to its inherent toxicity. Furthermore, nanomaterials developed from Te have not garnered adequate research attention. Conversely, nanomaterials fashioned using biomolecules augment their biological efficacy and applicability. Therefore, the present work focuses on synthesizing the tellurium nanoparticles (Te NPs) using the antioxidant molecule gallic acid (GA) and evaluating their biological activity and toxicity for the first time. The study evidenced that GA-Te NPs are spherical and monodispersed, with an average size of 19.74 ± 5.3 nm. XRD analysis confirmed a hexagonal crystalline structure for GA-Te NPs, and FTIR analysis evidenced the capping of GA on Te NPs. GA-Te NPs (MIC: 1.56 µg/mL) strongly reduce the growth and biofilm formation of S. aureus, E. coli, and S. enterica. Additionally, GA-Te NPs at a concentration of 50 µg/mL cause a significant level of toxicity in BT474 breast cancer cells but not in NIH3T3 cells. Unexpectedly, GA-Te NPs at concentrations <250 µg/mL do not cause hemolysis in red blood cells (RBC) Besides, the way of utilizing the lower concentrations of therapeutics could result in ecological safety. Therefore, the study concludes that GA-Te NPs could be used as potential multifunctional agents.

The amelioration and improvement effects of modified biochar derived from Spartina alterniflora on coastal wetland soil and Suaeda salsa growth.

Exotic species Spartina alterniflora (S. alterniflora) are widely invaded in the coastal zones of China and threaten the native ecosystem functions. In this study, phosphorus-magnesium modified BC (P-Mg modified BC) included PA-Mg-BC and DAP-Mg-BC derived from S. alterniflora were successfully prepared by co-pyrolysis of biomass and diammonium phosphate (DAP) or phosphoric acid (PA) and magnesium oxide (MgO). The preparation process markedly improved the surface morphologies, P loading amount, and P-containing functional groups of modified BC. The characterization results indicated that stable and low-solubility Mg-P complex formed on the surface of PA-Mg-BC and DAP-Mg-BC, which delayed the rapid release of P. Moreover, the MgO improved the buffering capacity of PA-Mg-BC and DAP-Mg-BC to competitive anions (SO42- and CO32-) during P release. Meanwhile, pot experiment showed that the suitable applications of PA-Mg-BC and DAP-Mg-BC could improve soil quality and fertility by enhancing SOC, DOC, TN, TP and AP contents, as well as ß-glucosidase activities. The amended soil pH and salinity compared to the original soil also declined through precipitation and acid-base neutralization. In addition, P-Mg modified BC could improve bacterial community structure and promote the growth and biomass of Suaeda salsa (S. salsa). This study could provide a feasible method for realizing ecological restoration of coastal wetland and resource utilization of S. alterniflora.

The environmental risk assessment of constructed wetlands filled with iron and manganese ores in typical antibiotic treatment.

Constructed wetlands (CWs) is considered as an efficient and environmentally friendly technology for advanced wastewater treatment to eliminate organic pollutants such as sulfamethoxazole (SMX) and trimethoprim (TMP). Iron (Fe) and manganese (Mn) ores have attracted more and more attention as CWs substrates in treating SMX and TMP, but the potentially negative environmental effects of wetland effluents, ore contaminants leached from the substrates and the risk of transmission of antibiotic resistance genes (ARGs) are still not clear. Three CW groups with different substrates (river sand (C-CW), Fe ore (Fe-CW), and Mn ore (Mn-CW)) were set up to evaluate the average removal rates and environmental risk in treating wastewater containing SMX and TMP. The results showed that the average removal rates of SMX and TMP by Fe-CW and Mn-CW were significantly higher than that of C-CW by 12.46%, 6.59% and 38.93%,15.39% respectively (p < 0.05), suggesting that both Fe and Mn ores facilitated the removal of antibiotics. However, the least abundance of ARGs was found in the layer of Fe ore at the middle layer (ML) in Fe-CW among all CWs, which suggested that Fe ore could reduce the risk of ARGs transmission. Although the environmental risk of Fe-CW and Mn-CW effluent was low, Fe-CW effluent inhibited the growth of Chlorella in both 48h and 72h experiments, while Mn-CW effluent showed an inhibitory effect in 48h and then promoted the growth in 72h. Meanwhile, these findings offer valuable insights for wetland health assessment and substrate selection for CWs.

Arbuscular mycorrhizal symbiosis alleviates arsenic phytotoxicity in flooded Iris tectorum Maxim. dependent on arsenic exposure levels.

Arsenic (As) pollution in wetlands has emerged as a serious global concern, posing potential threat to the growth of wetland plants. Arbuscular mycorrhizal fungi (AMF) can alleviate As phytotoxicity to host plants, but their ecological functions in wetland plants under flooding conditions remain largely unknown. Thus, a pot experiment was conducted using Rhizophagus irregularis and Iris tectorum Maxim. exposed to light (15 and 30 mg/kg As) and high (75 and 100 mg/kg As) levels of As, to investigate the intrinsic mechanisms underlying the effects of mycorrhizal inoculation on plant As tolerance under flooding conditions. The mycorrhizal colonization rates ranged from 31.47 ± 3.92 % to 60.69 ± 5.58 %, which were higher than the colonization rate (29.55 ± 13.60%) before flooding. AMF significantly increased biomass of I. tectorum under light As levels, together with increased phosphorus (P) and As uptake. Moreover, expression of arsenate reductase gene RiarsC and a trace of dimethylarsenic (1.87 mg/kg in shoots) were detected in mycorrhizal plants, suggesting As transformation and detoxification by AMF exposed to light levels of As. However, under high As levels, AMF inhibited As translocation from roots to shoots, and facilitated the formation of iron plaque. The immobilized As concentrations in iron plaque of mycorrhizal plants were respectively 1133.68 ± 179.17 mg/kg and 869.11 ± 248.90 mg/kg at 75 and 100 mg/kg As addition level, both significantly higher than that in non-inoculated plants. Irrespective of As exposure levels, mycorrhizal symbiosis decreased soil As bioavailability. Overall, the study provides insights into the alleviation of As phytotoxicity in natural wetland plants through mycorrhizal symbiosis, and potentially indicates function diversity of AMF under flooding conditions and As stress, supporting the subsequent phytoremediation and restoration of As-contaminated wetlands.

Single-cell RNA sequencing atlas of peripheral blood mononuclear cells from subjects with coronary artery disease.

BACKGROUND: The landscape of specific peripheral circulating immune cell subsets at the single-cell level in the occurrence and development of coronary artery disease (CAD) remains poorly understood. METHODS: We conducted single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) from subjects with CAD (n = 3), and controls (n = 3), as well as downstream analysis including cell- and gene-level approaches. This explored the characteristics of peripheral circulating immune cells between CADs and controls by means of Uniform manifold approximation and projection (UMAP), Monocle3 package, CellPhoneDB, and single-cell regulatory network inference and clustering (SCENIC). PBMCs were used as clinical samples for validating our findings by qRT-PCR. RESULTS: We identified 33 cell clusters among 67,447 cells, including monocytes, T cells, B cells, NK cells, and platelets. The significant difference in the abundance of the 33 clusters of cell type between CADs group and controls group was not found. The JUN was shared in cluster 0, 11,13, and 24 from differential expression genes analysis and SCENIC analysis in monocyte clusters between CAD and controls. Besides, JUN was validated to be significantly upregulated in the CAD group (p = 0.018) and may act as a potential diagnostic biomarker and independent predictor of CAD. CONCLUSIONS: Our study offered a detailed profiling of single-cell RNA sequencing of PBMCs from subjects with CADs and controls. These data provide a line of evidence that the JUN signaling pathway may be a potential diagnostic and therapeutic molecule target for CAD.

First Natural Yeast Strain Trichosporon asahii HZ10 with Robust Flavonoid Productivity and Its Potential Biosynthetic Pathway.

Flavonoids are generally thought to be essential plant natural products with diverse bioactivities and pharmacological effects. Conventional approaches for the industrial production of flavonoids through plant extraction and chemical synthesis face serious economic and environmental challenges. Searching for natural robust flavonoid-producing microorganisms satisfying green and sustainable development is one of the good alternatives. Here, a natural yeast, Trichosporon asahii HZ10, isolated from raw honeycombs, was found to accumulate 146.41 mg/L total flavonoids intracellularly. Also, T. asahii HZ10 represents a broad flavonoid metabolic profiling, covering 40 flavonoids, among which nearly half were silibinin, daidzein, and irigenin trimethyl ether, especially silibinin occupying 21.07% of the total flavonoids. This is the first flavonoid-producing natural yeast strain worldwide. Furthermore, T. asahii HZ10-derived flavonoids represent favorable antioxidant activities. Interestingly, genome mining and transcriptome analysis clearly showed that T. asahii HZ10 possibly evolves a novel flavonoid synthesis pathway for the most crucial step of flavonoid skeleton synthesis, which is different from that in plants and filamentous fungi. Therefore, our results not only enrich the diversity of the natural flavonoid biosynthesis pathway but also pave an alternative way to promote the development of a synthetic biology strategy for the microbial production of flavonoids.

Assembly and Characterization of the Mitochondrial Genome of Fallopia aubertii (L. Henry) Holub.

BACKGROUND: Fallopia aubertii (L. Henry) Holub is a perennial semi-shrub with both ornamental and medicinal value. The mitochondrial genomes of plants contain valuable genetic traits that can be utilized for the exploitation of genetic resources. The parsing of F. aubertii mitochondrial genome can provide insight into the role of mitochondria in plant growth and development, metabolism regulation, evolution, and response to environmental stress. METHODS: In this study, we sequenced the mitochondrial genome of F. aubertii using the Illumina NovaSeq 6000 platform and Nanopore platform. We conducted a comprehensive analysis of the mitochondrial genome of F. aubertii, which involved examining various aspects such as gene composition, repetitive sequences, RNA editing sites, phylogeny, and organelle genome homology. To achieve this, we employed several bioinformatics methods including sequence alignment analysis, repetitive sequence analysis, phylogeny analysis, and more. RESULTS: The mitochondrial genome of F. aubertii has 64 genes, including 34 protein-coding genes (PCGs), three rRNAs, and 27 tRNAs. There were 77 short tandem repeat sequences detected in the mitochondrial genome, five tandem repeat sequences identified by Tandem Repeats Finder (TRF), and 50 scattered repeat sequences observed, including 22 forward repeat sequences and 28 palindrome repeat sequences. A total of 367 RNA coding sites were predicted in PCGs, with the highest number (33) found within ccmB. Ka/Ks values estimated for mitochondrial genes of F. aubertii and three closely related species representing Caryophyllales were less than 1 for most of the genes. The maximum likelihood evolutionary tree showed that F. aubertii and Nepenthes ×ventrata are most closely related. CONCLUSIONS: In this study, we obtained basic information on the mitochondrial genome of F. aubertii and this study investigated repeat sequences and homologous segments, predicted RNA editing sites, and utilized the Ka/Ks ratio to estimate the selection pressure on mitochondrial genes of F. aubertii. We also discussed the systematic evolutionary position of F. aubertii based on mitochondrial genome sequences. Our study revealed variations in the sequence and structure of mitochondrial genomes in Caryophyllales. These findings are of great significance for identifying and improving valuable plant traits and serve as a reference for future molecular studies of F. aubertii.