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1.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(7): 749-754, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-32669173

RESUMO

OBJECTIVE: To study the clinical features of children with adenovirus pneumonia and hemophagocytic lymphohistiocytosis (HLH). METHODS: A retrospective analysis was performed on the mediacal data of 7 children with adenovirus pneumonia and HLH from March to September, 2019. RESULTS: The age of these children ranged from 11 months to 5 years, and among these children, 5 were aged <2 years and 5 were boys. None of these children had underlying diseases. All children were hospitalized due to persistent high fever and cough, and the peak temperature of fever was 39°C to 41°C. With disease progression, 7 children developed hepatomegaly and 6 developed splenomegaly. Routine blood test results showed reductions in two or three lineages of blood cells, with increases in serum ferritin (SF), C-reactive protein (CRP), procalcitonin (PCT), and lactate dehydrogenase (LDH). Phagocytosis of blood cells was observed in 6 children. Radiological examination of lungs showed pneumonia changes. All 7 children were diagnosed with human adenovirus type 7 infection based on pathogenic metagenome detection. No abnormality was found by HLH gene detection and the children were diagnosed with secondary HLH. All children received intravenous immunoglobulin. Among these children, 4 received dexamethasone and etoposide chemotherapy, 3 received dexamethasone alone, and 4 received plasma exchange. Of the 7 children, 2 died and 5 were recovered. Compared with those who survived, the children who died had significantly greater reductions in the three lineages of blood cells and significantly greater increases in serum levels of CRP, PCT, SF, and LDH. CONCLUSIONS: The children with adenovirus pneumonia and HLH have main clinical features of persistent high fever, progressive reductions in two or three lineages of peripheral blood cells, and involvement of other organ systems, including hepatosplenomegaly. Significant increases in serum levels of CRP, PCT, SF, and LDH may suggest a poor prognosis.


Assuntos
Linfo-Histiocitose Hemofagocítica , Adenoviridae , Pré-Escolar , Etoposídeo , Feminino , Humanos , Imunoglobulinas Intravenosas , Lactente , Masculino , Estudos Retrospectivos
2.
Huan Jing Ke Xue ; 41(5): 2367-2372, 2020 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608855

RESUMO

An integrated process uses an anaerobic baffled reactor combined with a fully mixed reactor (ABR-CSTR) as a test carrier for low-carbon, high-ammonia nitrogen (NH4+-N ≥ 200 mg·L-1) wastewater under continuous flow operating conditions; the normal anaerobic sludge in different compartments is subjected to domestication and cultivation to realize denitrifying phosphorus removal, partial nitritation, and anaerobic ammonium oxidation, thereby achieving the coupling effect of the three. Partial nitritation was successfully achieved in the A4 (CSTR) section by the strategy of limited oxygen (dissolved oxygen DO=0.8 mg·L-1) and intermittent aeration (exposure ratio=30 min:30 min) after 30 days. Subsequently, a strategy of shortening the hydraulic retention time (HRT) was adopted to achieve a stable operation of partial nitritation, and a stable influent substrate of NO2--N/NH4+-N 1.0-1.1 was provided for anaerobic ammonium oxidation. The anaerobic ammonium oxidation function was achieved after 154 days in the A5 and A6 compartments. The removal rates of NH4+-N and NO2--N were 94% and 97%, respectively, and the NO3--N concentration in the effluent was stable at 22 mg·L-1. The denitrifying phosphorus removal function was successfully achieved in the A1-A3 compartments by using NOx--N in the reflux as an electron acceptor. The removal rate of PO43--P was 77%. The integrated process was successfully coupled through 175 days, achieving simultaneous removal of C, N, and P.

3.
Reprod Biol Endocrinol ; 18(1): 42, 2020 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-32398082

RESUMO

BACKGROUND: Follicular fluid is an important external environment for the growth and development of oocytes. A thorough identification of specific components in follicular fluid can better the existing understand of intracellular signal transduction and reveal potential biomarkers of oocyte health in women undergoing assisted reproductive therapy. To study on follicular fluid metabolomics components at different ages based on lipid metabolism, we have adopted a new method of SWATH to MRM(the sequential window acquisition of all theoretical fragment-ion spectra to multiple reaction monitor)metabolomics to provide extensive coverage and excellent quantitative data. This was done to investigate the differences in follicular fluid of patients undergoing in vitro fertilization (IVF) and embryo transfer in different age groups and to further explore the relationship between follicular fluid, age and reproductive function. METHOD: A combination of Ultra-high-performance liquid chromatography and high resolution mass spectrometry techniques were used to analyze the follicular fluid of 230 patients enrolled for the IVF cycle. The patients were of different ages grouped into two groups:the younger and older patients.The obtained multidimensional chromatographic data were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The charge ratios and mass numbers enabled for the identification of different fragments in the samples. Matching information obtained through database search and the fragment information obtained by fragment ion scan structurally identified substances in the samples. This was used to determine the differential compounds. RESULTS: The quality of oocytes decline with age,and the lipid composition in follicular fluid also changes,The lipid metabolism that changes with age may be related to the quality of oocytes.The main differences were in lipid metabolites. Some were up-regulated: Arachidonate, LysoPC(16:1), LysoPC(20:4) and LysoPC(20:3) while others were down-regulated: LysoPC(18:3) and LysoPC(18:1). CONCLUSIONS: Metabolomic analysis of follicular fluid revealed that with the increase in age, several differential metabolites are at play. Among these metabolites, lipid metabolism undergoes significant changes that affect the development of oocytes thus causing reduced fertility in older women. These differential metabolites related to follicular development may provide possible detection and treatment targets for promoting oocyte health, and provide scientific basis for understanding the environment of oocyte development.

4.
Sci Total Environ ; 730: 138952, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32388374

RESUMO

It was difficult to obtain a stable and efficient biological nutrient removal for high-strength wastewater treatment, the possibility of exploiting innovative CANDPR process, integrating biofilm-based completely autotrophic nitrogen removal over nitrite (CANON) with denitrifying phosphorus removal (DPR) was evaluated to resolve the difficulty. Results revealed that the excellent NH4+-N, PO43--P and COD removal efficiencies of 96%, 96% and 91%, were achieved respectively under a high nitrogen loading rate (0.79 kg·m-3·d-1) without adding organic matters during 320 days operation. Promoting NOx--N recirculation demonstrated as an efficient strategy for further nutrient depletion, facilitating the enhanced NO3--N removal to 100% with the considerably high P-uptake performance. Batch tests confirmed that denitrifying phosphorus accumulating organisms (DPAOs) using NO3--N as electron acceptors accounting for 68% in total PAOs. Dechloromonas was identified as dominating genus in DPR, while Nitrosomonas (1.31%), Candidatus_Kuenenia (5.53%) and Candidatus_Brocadia (1.77%) contributed to the desirable nitrogen removal, indicating that cooperative consortia of DPAOs, AOB and AnAOB were harvested during long-term operation. The CANDPR process was verified to be energy-saving and treatment-reliable for renovating of existing plants.


Assuntos
Biofilmes , Microbiota , Reatores Biológicos , Desnitrificação , Nitrogênio , Nutrientes , Fósforo , Esgotos , Eliminação de Resíduos Líquidos , Águas Residuárias
5.
Chin Med J (Engl) ; 133(10): 1211-1220, 2020 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-32433053

RESUMO

BACKGROUND: Pancreatic cancer (PC) is a highly deadly malignancy with few effective therapies. We aimed to unmask the role that long non-coding RNA small nucleolar RNA host gene 6 (SNHG6) plays in PC cells by targeting far upstream element binding protein 1 (FUBP1) via microRNA-26a-5p (miR-26a-5p). METHODS: SNHG6 expression was predicted by bioinformatics, followed by verification via reverse transcription quantitative polymerase chain reaction. Then, the interactions among SNHG6, miR-26a-5p, and FUBP1 were detected through online software analysis, dual luciferase reporter assay and RNA pull-down. After that, cells were treated with different small interfering RNAs and/or mimic to determine the interactions among SNHG6, miR-26a-5p, and FUBP1 and their roles in PC cells. Finally, the role of SNHG6 in tumor growth in vivo was evaluated by measuring the growth and weight of transplanted tumors in nude mice. A t-test, one-way and two-way analysis of variance were used for data analysis. RESULTS: Compared with that in normal tissues, SNHG6 was highly expressed in PC tissues (1.00 ±â€Š0.05 vs. 1.56 ±â€Š0.06, t = 16.03, P < 0.001). Compared with that in human pancreatic duct epithelial cells (HPDE6-C7), SNHG6 showed the highest expression in PANC-1 cells (1.00 ±â€Š0.06 vs. 3.87 ±â€Š0.13, t = 34.72, P < 0.001) and the lowest expression in human pancreatic cancer cells (MIAPaCa-2) (1.00 ±â€Š0.06 vs. 1.41 ±â€Š0.07, t = 7.70, P = 0.0015). Compared with the levels in the si-negative control group, SNHG6 (0.97 ±â€Š0.05 vs. 0.21 ±â€Š0.06, t = 16.85, P < 0.001), N-cadherin (0.74 ±â€Š0.05 vs. 0.41 ±â€Š0.04, t = 8.93, P < 0.001), Vimentin (0.55 ±â€Š0.04 vs. 0.25 ±â€Š0.03, t = 10.39, P < 0.001), and ß-catenin (0.62 ±â€Š0.05 vs. 0.32 ±â€Š0.03, t = 8.91, P < 0.001) were decreased, while E-cadherin (0.65 ±â€Š0.06 vs. 1.36 ±â€Š0.07, t = 13.34, P < 0.001) was increased after SNHG6 knockdown or miR-26a-5p overexpression, accompanied by inhibited cell proliferation, migration, and invasion. SNHG6 overexpression exerted the opposite effects. SNHG6 upregulated FUBP1 expression by sponging miR-26a-5p. Silencing SNHG6 blocked the growth of PC in vivo. CONCLUSION: Silencing SNHG6 might ameliorate PC through inhibition of FUBP1 by sponging miR-26a-5p, thus providing further supporting evidence for its use in PC treatment.

7.
Sci Total Environ ; 715: 137023, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32041004

RESUMO

It was difficult to obtain a stable and efficient short-cut denitrification and phosphorus removal process for domestic sewage treatment, therefore, the possibility of using granulation technology of nitritation sludge and intermittent aeration was evaluated to resolve the difficulty. Results showed that the nitrogen and phosphorus removal efficiencies of the system were 72% and 93%, respectively, under the condition of mode 3 (a period of 10 min with aeration 8 min and stop 2 min) with aeration energy consumption decreasing 20%. Microbial community revealed that Rhodocyclales was the dominant short-cut denitrifying phosphorus removing bacteria in the process, closely related to the performance of short-cut denitrifying phosphorus removal. Furthermore, Thauera and Denitratisoma belonging to the Rhodocyclales play a significant role of short-cut denitrifying phosphorus removal in the process. In addition, Aeromonas improved the performance of short-cut denitrifying phosphorus removal. Finally, nutrient removal efficiency was improved 8% in intermittent aeration mode 3 based on short-cut denitrifying phosphorus removal.


Assuntos
Desnitrificação , Reatores Biológicos , Estudos de Viabilidade , Nitrificação , Nitrogênio , Fósforo , Esgotos , Eliminação de Resíduos Líquidos
8.
Sci Rep ; 10(1): 3494, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32103100

RESUMO

Surface incident solar radiation (Rs) of reanalysis products is widely used in ecological conservation, agricultural production, civil engineering and various solar energy applications. It is of great importance to have a good knowledge of the uncertainty of reanalysis Rs products. In this study, we evaluated the Rs estimates from two representative global reanalysis (ERA-Interim and MERRA-2) using quality- controlled surface measurements from China Meteorological Administration (CMA) and Multi-layer Simulation and Data Assimilation Center of the Tibetan Plateau (DAM) from 2000 to 2009. Error causes are further analyzed in combination radiation products from the Earth's Radiant Energy System (CERES) EBAF through time series estimation, hotspot selection and Geodetector methods. Both the ERA-Interim and MERRA-2 products overestimate the Rs in China, and the MERRA-2 overestimation is more pronounced. The errors of the ERA-Interim are greater in spring and winter, while that of the MERRA-2 are almost the same in all seasons. As more quality-controlled measurements were used for validation, the conclusions seem more reliable, thereby providing scientific reference for rational use of these datasets. It was also found that the main causes of errors are the cloud coverage in the southeast coastal area, aerosol optical depth (AOD) and water vapor content in the Sichuan Basin, and cloud coverage and AOD in the northeast and middle east of China.

9.
Environ Sci Technol ; 54(7): 4495-4503, 2020 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-32108484

RESUMO

Solar ultraviolet-B (UVB) radiation reaching the earth's surface is increasing due to stratospheric ozone depletion. How the elevated UVB affects the trophic interactions is critical for predicting the ecosystem functioning under this global-scale stressor. Usually, inducible defenses in phytoplankton stabilize community dynamics within aquatic environments. To assess the effects of elevated UVB on induced defense, we examined the changes in antigrazer colony formation in Scenedesmus obliquus under environmentally relevant UVB. S. obliquus exposed to Daphnia infochemicals consistently formed multicelled colonies, traits confirmed to be adaptive under predation risk. However, the suppressed photochemical activity and the metabolic cost from colony formation resulted in the severer reductions in algal growth by UVB under predation risk. The transcriptions of key enzyme-encoding genes, regulating the precursor synthesis during polysaccharide production, were also inhibited by UVB. Combination of the reduced production of daughter cells and the ability of daughter cells to remain attached, the antigrazing colony formation was interrupted, leading to the dominant morphs of algal population shifting from larger-sized colonies to smaller ones at raised UVB. The present study revealed that elevated UVB will not only reduce the phytoplankton growth but also increase their vulnerability to predation, probably leading to potential shifts in plankton food webs.


Assuntos
Scenedesmus , Animais , Carboidratos , Daphnia , Ecossistema , Raios Ultravioleta
10.
Pharmacol Res ; 152: 104623, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31899315

RESUMO

Gastric cancer remains the second most common tumor in China. Modified-Bu-zhong-yi-qi decoction (mBYD) as an adjuvant therapy for gastric cancer patients after chemotherapy could significantly prolong the survival time of patients. However, the potential anticancer mechanism for mBYD has not been well characterized. Here, we conducted a comprehensive study of mBYD on a gastric cancer xenograft model with MFC cells in 615 mice and patients. Our results showed that the survival times of the 5-FU + mBYD and mBYD groups were significantly longer than that of the control group. Moreover, the 5-FU + mBYD group had a longer survival time than the 5-FU group. Flow cytometry revealed that the value of CD4+/CD8+ in the mBYD group increased and that the proportions of CD8+PD-1+ T cells and PD-1+Treg cells were decreased when compared to the control group. Compared with the 5-FU group, CD8+PD-1+ T cells and Treg cells were both decreased when 5-FU was combined with mBYD. Further analysis showed that mBYD inhibited PD-L1 expression by the PI3K/AKT pathway in gastric cancer. An in vitro study also showed that mBYD directly promoted the proliferation, activation and cytotoxicity of T lymphocytes. Meanwhile, mBYD reduced the upregulation of CD8+PD-1+ T cells induced by chemotherapy in patients with gastric cancer. In conclusion, mBYD could modulate peripheral immunity and suppress the immune escape of tumors, which may be a promising therapy for gastric cancer.

11.
Pharm Dev Technol ; 25(1): 68-75, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31544585

RESUMO

To improve the aqueous solubility and the oral bioavailability of a poorly water-soluble biologically active pentacyclic triterpenoid, ursolic acid (UA), ursolic acid-phospholipid complex (UA-PC) was prepared using solvent-assisted grinding method which is green and simple. The phospholipid complex was characterized by differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), scanning electron microscope (SEM), and transmission electron microscope (TEM), which confirmed the formation of the phospholipid complex. Specifically, compared with free UA, the formulation demonstrated over 276-fold higher aqueous solubility of UA and exhibited faster dissolution rate and higher cumulative dissolution percentages. Finally, the oral bioavailability of the prepared UA-PC was evaluated using Sprague-Dawley (SD) rats. Compared with free UA, the UA-PC exhibited considerable enhancement in the bioavailability with an increase in Cmax (183.80 vs 68.26 µg/l) and AUC 0-24 h (878.0 vs 212.1 µg·h/l), which was consistent with the in vitro results. This enhancement was attributed to the improvement of solubility and dissolution in vitro. Therefore, the method of solvent-assisted grinding appears to be an efficient approach for the preparation of UA-PC, and the prepared UA-PC showed a promising potential to overcome the limitation of poor oral bioavailability associated with low water solubility.


Assuntos
Fosfolipídeos/química , Solubilidade/efeitos dos fármacos , Solventes/química , Triterpenos/química , Administração Oral , Animais , Disponibilidade Biológica , Varredura Diferencial de Calorimetria/métodos , Masculino , Microscopia Eletrônica de Varredura/métodos , Ratos , Ratos Sprague-Dawley , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Triterpenos/farmacocinética , Água/química , Difração de Raios X/métodos
12.
Chemosphere ; 245: 125572, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31846786

RESUMO

In order to promote the application of anaerobic ammonium oxidation (Anammox) for municipal wastewater treatment, single and two-stage Anammox processes were started up for real low-strength wastewater treatment under similar conditions for the comparison. Results showed that the anaerobic baffled reactor (ABR)-Nitritation-Anammox and the ABR-Completely Autotrophic Nitrogen removal Over Nitrite (CANON) process took 75 days and 101 days to start up with a total nitrogen removal rate of 86-92% and 81-87% under steady state, respectively. The 16 S rRNA sequencing analysis revealed that the phylum of Proteobacteria dominated in CANON system and Anammox system with the relative abundance of 35.39% and 15.27%, respectively. Phylogenetic analysis showed that Anammox species, related to Ca. Brocadia Sinica JPN1 and Ca. Kuenenia stuttgartiensis, dominated in these two systems, respectively. The nitrogen removal performance of two-stage process was 5% higher than that of single stage process, while the start-up period and dominated Anammox species were different.


Assuntos
Eliminação de Resíduos Líquidos/métodos , Compostos de Amônio , Anaerobiose , Bactérias/genética , Reatores Biológicos/microbiologia , Nitritos , Nitrogênio , Oxirredução , Filogenia , Águas Residuárias/microbiologia
13.
Hum Brain Mapp ; 41(1): 67-79, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31517428

RESUMO

An increasing number of studies in patients with generalized tonic-clonic seizures (GTCS) have reported the alteration of functional connectivity (FC) in many brain networks. However, little is known about the underlying temporal variability of FC in large-scale brain functional networks in patients. Recently, dynamic FC could provide novel insight into the physiological mechanisms in the brain. Here, we recruited 63 GTCS and 65 age- and sex-matched healthy controls. Dynamic FC approaches were used to evaluate alterations in the temporal variability of FC in patients at the region- and network-levels. In addition, two kinds of brain templates (>102 and > 103 regions) and two kinds of temporal variability FC approaches were adopted to verify the stability of the results. Patients showed increased FC variability in regions of the default mode network (DMN), ventral attention network (VAN) and motor-related areas. The DAN, VAN, and DMN illustrated enhanced FC variability at the within-network level. In addition, increased FC variabilities between networks were found between the DMN and cognition-related networks, including the VAN, dorsal attention network and frontal-parietal network in GTCS. Meanwhile, the alterations in FC variability were relatively consistent across different methods and templates. Therefore, the consistent alteration of FC variability would reflect a dynamic restructuring of the large-scale brain networks in patients with GTCS. Overly frequent information communication among cognition-related networks, especially in the DMN, might play a role in the epileptic activity and/or cognitive dysfunction in patients.

14.
J Am Chem Soc ; 141(51): 20249-20260, 2019 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-31793304

RESUMO

Novel actinide cluster fullerenes, U2C2@Ih(7)-C80 and U2C2@D3h(5)-C78, were synthesized and fully characterized by mass spectrometry, single-crystal X-ray crystallography, UV-vis-NIR, nuclear magnetic resonance spectroscopy (NMR), X-ray absorption spectroscopy (XAS), Raman spectroscopy, IR spectroscopy, as well as density functional and multireference wave function calculations. The encapsulated U2C2 is the first example of a uranium carbide cluster featuring two U centers bridged by a C≡C unit. The U-C bond distances in these U2C2 clusters are in the range between 2.130 and 2.421 Å. While the U2C2 cluster in U2C2@C80 adopts a butterfly-shaped geometry with a U-C2-U dihedral angle of 112.7° and a U-U distance of 3.855 Å, the U-U distance in U2C2@C78 is 4.164 Å and the resulting U-C2-U dihedral angle is increased to 149.1°. The combined experimental and quantum-chemical results suggest that the formal U oxidation state is +4 in the U2C2 cluster, and each U center transfers three electrons to the C2n cage and one electron to C2. Different from the strong U═C covalent bonding reported for U2C@C80, the U-C bonds in U2C2 are less covalent and predominantly ionic. The C-C triple bond is somewhat weaker than in HCCH, and the C-C π bonds undergo donation bonding with the U centers. This work demonstrates that the combination of the unique encapsulation effect of fullerene cages and the variable oxidation states of actinide elements can lead to the stabilization of novel actinide clusters, which are not accessible by conventional synthetic methods.

15.
Nat Commun ; 10(1): 5552, 2019 12 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804474

RESUMO

Adding small nanoparticles (NPs) into polymer melt can lead to a non-Einstein-like decrease in viscosity. However, the underlying mechanism remains a long-standing unsolved puzzle. Here, for an all-polymer nanocomposite formed by linear polystyrene (PS) chains and PS single-chain nanoparticles (SCNPs), we perform large-scale molecular dynamics simulations and experimental rheology measurements. We show that with a fixed (small) loading of the SCNP, viscosity reduction (VR) effect can be largely amplified with an increase in matrix chain length [Formula: see text], and that the system with longer polymer chains will have a larger VR. We demonstrate that such [Formula: see text]-dependent VR can be attributed to the friction reduction experienced by polymer segment blobs which have similar size and interact directly with these SCNPs. A theoretical model is proposed based on the tube model. We demonstrate that it can well describe the friction reduction experienced by melt polymers and the VR effect in these composite systems.

16.
J Vet Sci ; 20(6): e74, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31775200

RESUMO

As an important zoonotic pathogen, Staphylococcus aureus has led to serious mastitis and endometritis in infected dairy cows. In this study, a total of 164 strains of S. aureus were isolated from dairy cows in Xinjiang Province, China, and subjected to assays to determine drug susceptibility and biofilm (BF) formation ability. Enterotoxin-related genes were detected, and the transcription levels of genes related to BF formation were determined by using reverse transcription-quantitative polymerase chain reaction. Moreover, the pathogenicity of isolates with different BF formation abilities was determined by measuring their hemolysis activity, half lethal dose (LD50) and organ bacterial load. The results showed that 86.0% of S. aureus isolates could form BF. Among them, 42.1% of the strains had weak BF formation ability, and most strains with a strong BF formation ability were ica gene carriers. The S. aureus isolates displayed multidrug resistance and their drug resistance was positively correlated with their BF formation ability. Moreover, 96.3% of the S. aureus isolates carried enterotoxin genes. Among them, the detection rates of the novel enterotoxin genes were higher than those of conventional enterotoxin genes. Furthermore, isolates with a strong BF formation ability had higher LD50 but lower hemolysis ability and organ bacterial load than those of the isolates with weak or no BF ability. However, isolates without BF ability produced more severe pathological changes than those of isolates with strong BF formation ability. These findings suggest that higher BF ability and presence of novel enterotoxin genes are important characteristics of S. aureus isolates from dairy cows in Xinjiang Province, China, and such isolates may pose potential threats to food safety.


Assuntos
Biofilmes , Doenças dos Bovinos/microbiologia , Farmacorresistência Bacteriana/genética , Enterotoxinas/química , Infecções Estafilocócicas/veterinária , Staphylococcus aureus/fisiologia , Staphylococcus aureus/patogenicidade , Animais , Antibacterianos/farmacologia , Bovinos , China , Indústria de Laticínios , Feminino , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/genética , Virulência
17.
Adv Sci (Weinh) ; 6(20): 1901352, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31637168

RESUMO

A new class of single-molecule magnets (SMMs) based on Dy-oxide clusterfullerenes is synthesized. Three isomers of Dy2O@C82 with C s(6), C 3v(8), and C 2v(9) cage symmetries are characterized by single-crystal X-ray diffraction, which shows that the endohedral Dy-(µ2-O)-Dy cluster has bent shape with very short Dy-O bonds. Dy2O@C82 isomers show SMM behavior with broad magnetic hysteresis, but the temperature and magnetization relaxation depend strongly on the fullerene cage. The short Dy-O distances and the large negative charge of the oxide ion in Dy2O@C82 result in the very strong magnetic anisotropy of Dy ions. Their magnetic moments are aligned along the Dy-O bonds and are antiferromagnetically (AFM) coupled. At low temperatures, relaxation of magnetization in Dy2O@C82 proceeds via the ferromagnetically (FM)-coupled excited state, giving Arrhenius behavior with the effective barriers equal to the AFM-FM energy difference. The AFM-FM energy differences of 5.4-12.9 cm-1 in Dy2O@C82 are considerably larger than in SMMs with {Dy2O2} bridges, and the Dy∙∙∙Dy exchange coupling in Dy2O@C82 is the strongest among all dinuclear Dy SMMs with diamagnetic bridges. Dy-oxide clusterfullerenes provide a playground for the further tuning of molecular magnetism via variation of the size and shape of the fullerene cage.

18.
Chem Commun (Camb) ; 55(76): 11462-11465, 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31490473

RESUMO

We report here a single-benzene based fluorescent framework, amino-terephthalonitrile, denoted SB-Fluor. This scaffold displays versatile emission wavelength tunability via structure modification, covering the full visible light spectrum, both in the solution and solid state. Moreover, one molecule, SBF3, exhibits polymorphism-dependent reversible mechanochromic luminescence.

19.
ACS Nano ; 13(9): 10279-10293, 2019 09 24.
Artigo em Inglês | MEDLINE | ID: mdl-31483606

RESUMO

Diabetic wound healing and angiogenesis remain a worldwide challenge for both clinic and research. The use of adipose stromal cell derived exosomes delivered by bioactive dressing provides a potential strategy for repairing diabetic wounds with less scar formation and fast healing. In this study, we fabricated an injectable adhesive thermosensitive multifunctional polysaccharide-based dressing (FEP) with sustained pH-responsive exosome release for promoting angiogenesis and diabetic wound healing. The FEP dressing possessed multifunctional properties including efficient antibacterial activity/multidrug-resistant bacteria, fast hemostatic ability, self-healing behavior, and tissue-adhesive and good UV-shielding performance. FEP@exosomes (FEP@exo) can significantly enhance the proliferation, migration, and tube formation of endothelial cells in vitro. In vivo results from a diabetic full-thickness cutaneous wound model showed that FEP@exo dressing accelerated the wound healing by stimulating the angiogenesis process of the wound tissue. The enhanced cell proliferation, granulation tissue formation, collagen deposition, remodeling, and re-epithelialization probably lead to the fast healing with less scar tissue formation and skin appendage regeneration. This study showed that combining bioactive molecules into multifunctional dressing should have great potential in achieving satisfactory healing in diabetic and other vascular-impaired related wounds.

20.
Life Sci ; 235: 116829, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31484042

RESUMO

BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is a severe liver disease, which influences the health of people worldwide. However, the specific mechanism of the disease remains unknown, and effective treatments are still lacking. It was reported that Nuclear enriched abundant transcript 1 (NEAT1) obviously was up-regulated in NAFLD model. But the role and underlying mechanism of NEAT1 in NAFLD is unclear. METHODS: HepG2 cells were treated by free fatty acids (FFA) and C57BL/6J mice were treated by high-fat diet to establish NAFLD in vitro and in vivo models, respectively. Cell transfection was applied to regulate the expression of NEAT1, ROCK1, and miR-146a-5p. Western blotting and qRT-PCR were used for measuring expression of protein and mRNA level, respectively. Dual luciferase assay was used to detect the target relationship. Oil Red O staining was used to measure the lipid accumulation. HE staining was used for observing pathological feature of liver tissues. RESULTS: High levels of NEAT1 and ROCK1, and low level of miR-146a-5p were identified in NAFLD models. NEAT1 could target miR-146a-5p to promote ROCK1 expression. Knockdown of NEAT1, overexpression of miR-146a-5p and knockdown of ROCK1 inhibited lipid accumulation through activating AMPK pathway. CONCLUSION: NEAT1 may regulate NAFLD through miR-146a-5p targeting ROCK1, and further affect AMPK/SREBP pathway. This study may provide a new thought for the treatment of NAFLD.


Assuntos
Metabolismo dos Lipídeos , MicroRNAs/metabolismo , Hepatopatia Gordurosa não Alcoólica/metabolismo , RNA Longo não Codificante/metabolismo , Quinases Associadas a rho/metabolismo , Animais , Linhagem Celular Tumoral , Dieta Hiperlipídica , Regulação para Baixo , Ácidos Graxos não Esterificados/farmacologia , Técnicas de Silenciamento de Genes , Humanos , Masculino , Camundongos , Transdução de Sinais , Transfecção , Regulação para Cima
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