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1.
Aging Cell ; : e13102, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32074399

RESUMO

In female mammals, the size of the initially established primordial follicle (PF) pool within the ovaries determines the reproductive lifespan of females. Interestingly, the establishment of the PF pool is accompanied by a remarkable programmed oocyte loss for unclear reasons. Although apoptosis and autophagy are involved in the process of oocyte loss, the underlying mechanisms require substantial study. Here, we identify a new role of lysine-specific demethylase 1 (LSD1) in controlling the fate of oocytes in perinatal mice through regulating the level of autophagy. Our results show that the relatively higher level of LSD1 in fetal ovaries sharply reduces from 18.5 postcoitus (dpc). Meanwhile, the level of autophagy increases while oocytes are initiating programmed death. Specific disruption of LSD1 resulted in significantly increased autophagy and obviously decreased oocyte number compared with the control. Conversely, the oocyte number is remarkably increased by the overexpression of Lsd1 in ovaries. We further demonstrated that LSD1 exerts its role by regulating the transcription of p62 and affecting autophagy level through its H3K4me2 demethylase activity. Finally, in physiological conditions, a decrease in LSD1 level leads to an increased level of autophagy in the oocyte when a large number of oocytes are being lost. Collectively, LSD1 may be one of indispensible epigenetic molecules who protects oocytes against preterm death through repressing the autophagy level in a time-specific manner. And epigenetic modulation contributes to programmed oocyte death by regulating autophagy in mice.

2.
Water Environ Res ; 92(2): 302-309, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31560145

RESUMO

A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons. In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin. The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 . The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 . Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges. Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution. When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively. The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants. PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment. The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 . The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.


Assuntos
Caulim , Purificação da Água , Floculação , Glucanos , Água
3.
Nat Chem ; 11(12): 1085-1090, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31758160

RESUMO

Air pollution by nitrogen oxides, NOx, is a major problem, and new capture and abatement technologies are urgently required. Here, we report a metal-organic framework (Manchester Framework Material 520 (MFM-520)) that can efficiently confine dimers of NO2, which results in a high adsorption capacity of 4.2 mmol g-1 (298 K, 0.01 bar) with full reversibility and no loss of capacity over 125 cycles. Treatment of NO2@MFM-520 with water in air leads to a quantitative conversion of the captured NO2 into HNO3, an important feedstock for fertilizer production, and fully regenerates MFM-520. The confinement of N2O4 inside nanopores was established at a molecular level, and the dynamic breakthrough experiments using both dry and humid NO2 gas streams verify the excellent stability and selectivity of MFM-520 and confirm its potential for precious-metal-free deNOx technologies.

4.
Biosci Rep ; 39(11)2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31696224

RESUMO

OBJECTIVES: We aimed to meta-analyze the results of published randomized controlled trials to test the hypothesis that low vitamin D supplement is associated with an increased risk of cancer incidence and mortality. METHODS: Randomized controlled trials that explored the association between vitamin D supplement and cancer incidence or mortality as primary outcomes were identified through searching the PubMed and EMBASE. Literature search and data extraction were performed independently and in duplicate. RESULTS: Ten randomized controlled trials pooled in 81362 participants. The incidence rate of cancer was 9.16% (3716 cases) and 9.29% (3799 cases) in vitamin D intervention group and placebo group, respectively, resulting in a nonsignificant relative risk (RR) (95% confidence interval (95% CI)) of 0.99 (0.94-1.03) (P=0.532). The mortality rate of cancer was 2.11% (821 cases) and 2.43% (942 cases) in vitamin D intervention group and placebo group, respectively, resulting in a significant reduction in risk (RR = 0.87, 95% CI: 0.79-0.95, P=0.003). There was no observable heterogeneity or publication bias. Subgroup analyses revealed that history of cancer, extra use of vitamin D and calcium supplement were potential sources of heterogeneity. CONCLUSIONS: Our findings support a beneficial effect of vitamin D supplement on lowering cancer mortality, especially in subpopulations with no history of cancer, extra use of vitamin D, or calcium supplement.

5.
FASEB J ; 33(12): 14703-14716, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31693862

RESUMO

In female mammals, the majority of primordial follicles (PFs) are physiologically quiescent, and only a few of them are activated and enter the growing follicle pool. Specific molecules, such as mammalian target of rapamycin (mTOR) and the serine/threonine kinase Akt (AKT), have been proven to be important for PF activation. However, how the transcription of these genes is regulated is not clear. Although activators of mTOR or AKT have been successfully used to rescue the fertility of patients with premature ovarian insufficiency, the low efficacy and unclear safety profile of these drugs hinder their clinical use in the in vitro activation (IVA) of PFs. Here, sirtuin 1 (SIRT1), an NAD-dependent deacetylase, was demonstrated to activate mouse PFs independent of its deacetylase activity. SIRT1 was prominently expressed in pregranulosa cells (pGCs) and oocytes, and its expression was increased during PF activation. PF activation was achieved by either up-regulating SIRT1 with a specific activator or overexpressing SIRT1. Moreover, SIRT1 knockdown in oocytes or pGCs could significantly suppress PF activation. Further studies demonstrated that SIRT1 enhanced both Akt1 and mTOR expression by acting more as a transcription cofactor, directly binding to the respective gene promoters, than as a deacetylase. Importantly, we explored the potential clinical applications of targeting SIRT1 in IVA via short-term treatment of cultured ovaries from mice and human ovarian tissues to activate PFs by applying the SIRT1 activator resveratrol. RSV-induced IVA could be a candidate strategy to develop more efficient procedures for future clinical treatment of infertility.-Zhang, T., Du, X., Zhao, L., He, M., Lin, L., Guo, C., Zhang, X., Han, J., Yan, H., Huang, K., Sun, G., Yan, L., Zhou, B., Xia, G., Qin, Y., Wang, C. SIRT1 facilitates primordial follicle recruitment independent of deacetylase activity through directly modulating Akt1 and mTOR transcription.

6.
Nat Commun ; 10(1): 5117, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31712651

RESUMO

The integration of luminescence and chirality in easy-scalable metal-organic frameworks gives rise to the development of advanced luminescent sensors. To date, the synthesis of chiral metal-organic frameworks is poorly predictable and their chirality primarily originates from components that constitute the frameworks. By contrast, the introduction of chirality into the pores of metal-organic frameworks has not been explored to the best of our knowledge. Here, we demonstrate that chirality can be introduced into an anionic Zn-based metal-organic framework via simple cation exchange, yielding dual luminescent centers comprised of the ligand and Tb3+ ions, accompanied by a chiral center in the pores. This bifunctional material shows enantioselectivity luminescent sensing for a mixture of stereoisomers, demonstrated for Cinchonine and Cinchonidine epimers and amino alcohol enantiomers, from which the quantitative determination of the stereoisomeric excess has been obtained. This study paves a pathway for the design of multifunctional metal-organic framework systems as a useful method for rapid sensing of chiral molecules.

7.
Inorg Chem ; 58(20): 14145-14150, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31566954

RESUMO

We report a comparative study of the binding of I2 (iodine) in a pair of redox-active metal-organic framework (MOF) materials, MFM-300(VIII) and its oxidized, deprotonated analogue, MFM-300(VIV). Adsorption of I2 in MFM-300(VIII) triggers a host-to-guest charge-transfer, accompanied by a partial (∼30%) oxidation of the VIII centers in the host framework and formation of I3- species residing in the MOF channels. Importantly, this charge-transfer induces a significant enhancement in the electrical conductivity (Δσ = 700000) of I2@MFM-300(VIII/IV) in comparison to MFM-300(VIII). In contrast, no host-guest charge-transfer or apparent change in the conductivity was observed upon adsorption of I2 in MFM-300(VIV). High-resolution synchrotron X-ray diffraction of I2@MFM-300(VIII/IV) confirms the first example of self-aggregation of adsorbed iodine species (I2 and I3-) into infinite helical chains within a MOF.

8.
J Biomed Inform ; 100: 103319, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31655272

RESUMO

To provide natural simulated objects and intuitive user interaction in medical education and training, we propose a naked eye 3D display and interaction system. The current 3D rendering algorithms for naked eye 3D displays are not suitable for medical use, due to the requirements of displaying and interacting with high quality medical images and simulating soft tissues. Because the traditional 3D rendering procedure and vertex indexing in collision detection require substantial computing power when using a naked eye 3D display, the current method cannot achieve fluent displays and interactions. Thus, we develop a novel octree-based 3D rendering and interaction algorithm for high quality medical models to improve the rendering rate and obtain smooth human machine interactions when using the naked eye 3D display device. We also valuate the soft-body phantom simulation of the naked eye 3D display device by combining the traditional 3D rendering algorithm with the elastic 3D simulation to simulate deformable tissues. We integrate an incremental interaction method and a Kalman filter-based hand tracking method to achieve a larger user interaction range and robust hand tracking. We used the proposed system to perform human-computer interactions with rigid phantoms and soft-body phantoms. The experimental results showed that the proposed rendering algorithm for rigid phantoms could achieve higher rendering performance (50 FPS) than the traditional rendering algorithm (9.8 FPS). The user experiments showed that the 3D simulation system equipped with the enhanced rendering algorithm could achieve fluent interactions when using the naked eye 3D display, thus promoting education experiences and reducing task completion times.

9.
Nat Mater ; 18(12): 1358-1365, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31611671

RESUMO

Emissions of SO2 from flue gas and marine transport have detrimental impacts on the environment and human health, but SO2 is also an important industrial feedstock if it can be recovered, stored and transported efficiently. Here we report the exceptional adsorption and separation of SO2 in a porous material, [Cu2(L)] (H4L = 4',4‴-(pyridine-3,5-diyl)bis([1,1'-biphenyl]-3,5-dicarboxylic acid)), MFM-170. MFM-170 exhibits fully reversible SO2 uptake of 17.5 mmol g-1 at 298 K and 1.0 bar, and the SO2 binding domains for trapped molecules within MFM-170 have been determined. We report the reversible coordination of SO2 to open Cu(II) sites, which contributes to excellent adsorption thermodynamics and selectivities for SO2 binding and facile regeneration of MFM-170 after desorption. MFM-170 is stable to water, acid and base and shows great promise for the dynamic separation of SO2 from simulated flue gas mixtures, as confirmed by breakthrough experiments.

10.
Opt Express ; 27(15): 20421-20434, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31510136

RESUMO

We propose a novel full-parallax autostereoscopic display based on a lenticular tracking method to achieve separation between the viewing angle and image resolution and to improve these two parameters simultaneously. The proposed method enables the viewing angle to be independent of the image resolution and has the potential to solve the long-term trade-off problem in integral photography. By employing the lenticular lens array instead of the micro-lens array in integral photography with viewing tracking, the proposed method shows a high-image resolution and wide viewing angle 3D display with full parallax. A real-time tracking and rendering algorithm for the display method is also proposed in this study. The experimental results, compared with those of the conventional integral photography display and the tracking-based integral photography display, demonstrate the feasibility of this lenticular tracking display technology and its advantages in display resolution and viewing angle, suggesting its potential in practical three-dimensional applications.

11.
Water Res ; 165: 115023, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31472336

RESUMO

Purine and pyrimidines are present an important pool of dissolved organic nitrogen in aqueous medias and also precursors of disinfection byproducts. The degradation kinetics of cytosine and adenine-model pyrimidine and purine compounds-were investigated along with their transformation pathways leading to the formation of disinfection byproducts during two typical multi-barrier disinfection processes: UV irradiation and UV/chlorine pretreatment followed by post-chlorination. UV irradiation followed by post-chlorination enhanced the degradation of cytosine and adenine (by 17.1 and 26.1%, respectively), but it also generated more byproduct precursors compared to chlorination alone. The presence of reactive species in the UV/chlorine treatment greatly enhanced cytosine and adenine degradation (by 61.8 and 123.0%) but generated even more disinfection byproducts. Compared to 24 h chlorination, the concentrations of byproducts increased by up to 361.6% for cytosine and 85.1% for adenine with longer UV/chlorine treatment (from 2 to 30 min). Thirty minutes of combined UV/chlorine treatment decreased the total organic chlorine produced from cytosine by 34.4% (from 233.8 to 153.3 µg Cl L-1) but it increased byproduct generation by 68.3% compared with 24 h of simple chlorination. The TOCl from adenine increased by 50.0% (from 9.2 to 18.4 µg Cl L-1) but byproduct generation was 11.0% less after 30 min of UV/chlorine pretreatment followed by 24 h of chlorination. The intermediates generated were analyzed in detail and multiple transformation pathways leading to byproduct formation are proposed.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Cloro , Desinfecção , Halogenação , Purinas , Pirimidinas , Raios Ultravioleta
12.
Chemistry ; 25(62): 14117-14122, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31414507

RESUMO

The design of electrode materials with rational core/shell structures is promising for improving the electrochemical properties of supercapacitors. Hence, hierarchical FeCo2 S4 @FeNi2 S4 core/shell nanostructures on Ni foam were fabricated by a simple hydrothermal method. Owing to their structure and synergistic effect, they deliver an excellent specific capacitance of 2393 F g-1 at 1 A g-1 and long cycle lifespan as positive electrode materials. An asymmetric supercapacitor device with FeCo2 S4 @FeNi2 S4 as positive electrode and graphene as negative electrode exhibited a specific capacitance of 133.2 F g-1 at 1 A g-1 and a high energy density of 47.37 W h kg-1 at a power density of 800 W kg-1 . Moreover, the device showed remarkable cycling stability with 87.0 % specific-capacitance retention after 5000 cycles at 2 A g-1 . These results demonstrate that the hierarchical FeCo2 S4 @FeNi2 S4 core/shell structures have great potential in the field of electrochemical energy storage.

13.
Virus Genes ; 55(6): 834-842, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31420829

RESUMO

A novel Vibrio phage, P23, belonging to the family Siphoviridae was isolated from the surface water of the Yellow Sea, China. The complete genome of this phage was determined. A one-step growth curve showed that the latent period was approximately 30 min, the burst size was 24 PFU/cell, and the rise period was 20 min. The phage is host specific and is stable over a range of pH (5-10) and temperatures (4-65 °C). Transmission electron microscopy showed that phage P23 can be categorized into the Siphoviridae family, with an icosahedral head of 60 nm and a long noncontractile tail of 144 nm. The genome consisted of a linear, double-stranded 40.063 kb DNA molecule with 42.5% G+C content and 72 putative open reading frames (ORFs) without tRNA. The predicted ORFs were classified into six functional groups, including DNA replication, regulation and nucleotide metabolism, transcription, phage packaging, phage structure, lysis, and hypothetical proteins. The Vibrio phage P23 genome is a new marine Siphoviridae-family phage genome that provides basic information for further molecular research on interaction mechanisms between bacteriophages and their hosts.

14.
ACS Appl Mater Interfaces ; 11(31): 27982-27988, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31313899

RESUMO

Iodine detection is crucial for nuclear waste clean-up and first responder activities. For ease of use and durability of response, robust active materials that enable the direct electrical detection of I2 are needed. Herein, a large reversible electrical response is demonstrated as I2 is controllably and repeatedly adsorbed and desorbed from a series of metal-organic frameworks (MOFs) MFM-300(X), each possessing a different metal center (X = Al, Fe, In, or Sc) bridged by biphenyl-3,3',5,5'-tetracarboxylate linkers. Impedance spectroscopy is used to evaluate how the different metal centers influence the electrical response upon cycling of I2 gas, ranging from 10× to 106× decrease in resistance upon I2 adsorption in air. This large variation in electrical response is attributed not only to the differing structural characteristics of the MOFs but also to the differing MOF morphologies and how this influences the degree of reversibility of I2 adsorption. Interestingly, MFM-300(Al) and MFM-300(In) displayed the largest changes in resistance (up to 106×) yet lost much of their adsorption capacity after five I2 adsorption cycles in air. On the other hand, MFM-300(Fe) and MFM-300(Sc) revealed more moderate changes in resistance (10-100×), maintaining most of their original adsorption capacity after five cycles. This work demonstrates how changes in MOFs can profoundly affect the magnitude and reversibility of the electrical response of sensor materials. Tuning both the intrinsic (resistivity and adsorption capacity) and extrinsic (surface area and particle morphology) properties is necessary to develop highly reversible, large signal-generating MOF materials for direct electrical readout for I2 sensing.

15.
J Cancer ; 10(8): 1794-1799, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31205535

RESUMO

Background and Objectives: Emerging evidence indicates that hypertension is a potential risk and prognostic factor for cancer at many sites. Currently, no data are available on optimal blood pressure target in patients with resectable digestive tract cancer. Here, we did an exploratory analysis in 6865 patients from the FIESTA cohort to identify optimal blood pressure at baseline that can better predict digestive tract cancer-specific mortality risk postoperatively. Methods and Results: Patients were enrolled between January 2000 and December 2010, with follow-up ending in December 2015. All patients received no preoperative and postoperative chemotherapy or radiotherapy. Data were analyzed using Stata software and R language. Optimal cutting points were determined using survival tree analysis. After a median follow-up of 44.9 months, there were 2808 non-survivors and 4057 survivors. Per 10 mm Hg increment, baseline systolic blood pressure (SBP), diastolic blood pressure (DBP), pulse pressure and mean arterial pressure were associated with the significant risk of digestive tract cancer-specific mortality, even after adjusting for confounding factors (adjusted hazard ratio: 1.06, 1.08, 1.06 and 1.09, 95% confidence interval: 1.04-1.08, 1.04-1.12, 1.03-1.09 and 1.05-1.12, P<0.001, <0.001, <0.001 and <0.001, respectively). Patients with baseline SBP of 176 mm Hg or above and DBP of 100 mm Hg or above had poor survival outcomes (median survival time: 39.6 and 37.1 months, respectively). Conclusions: We provide evidence for the use of elevated blood pressure (SBP/DBP ≥176/100 mm Hg) before surgery as a powerful harbinger to predict the survival outcomes of digestive tract cancer patients postoperatively.

16.
Front Oncol ; 9: 427, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31192131

RESUMO

Objectives: We sought to determine the optimal cutting points for two inflammatory biomarkers, neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR), to assess their prognostic value in patients with postoperative digestive tract cancers overall and by cancer sites, and further to construct an inflammation-related index based on the two biomarkers and assess its predictive performance. Methods: Total 6,865 assessable patients with digestive tract cancers who underwent tumor resection were consecutively enrolled from Fujian Cancer Hospital between January 2000 and December 2010, including 2535/3012/1318 patients with esophageal/gastric/colorectal cancer. The latest follow-up (median: 44.9 months) ended in December 2015. Optimal cutting points were determined using survival tree analysis overall and by cancer sites. Results: Among all study patients, the optimal cutting points were 2.07 and 168.50 to define high and low NLR and PLR, respectively. High NLR (hazard ratio [HR]: 1.48, 95% confidence interval [CI]: 1.37-1.61) and high PLR (HR: 1.41, 95% CI: 1.29-1.53) were associated with a significantly increased risk for the mortality of digestive tract cancers as a whole. By cancer sites, effect-size estimates were comparable and statistically significant. Elevation over the selected optimal cutting points for both NLR and PLR was associated with 1.69-fold increased risk of cancer-specific mortality compared to patients with simultaneously low NLR and PLR among all study patients, and this association persisted by cancer sites, especially for gastric cancer. Conclusions: Our findings demonstrate that the preoperative integrated NLR and PLR, as an inflammation-related index, is a significant independent predictor for postoperative mortality in Chinese patients with digestive tract cancers both overall and by cancer sites.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31180870

RESUMO

Microarray gene expression data have become a topic of great interest for cancer classification and for further research in the field of bioinformatics. Nonetheless, due to the "large p, small n" paradigm of limited biosamples and high-dimensional data, gene selection is becoming a demanding task, which is aimed at selecting a minimal number of discriminatory genes associated closely with a phenotype. Feature or gene selection is still a challenging problem owing to its nondeterministic polynomial time complexity and thus most of the existing feature selection algorithms utilize heuristic rules. A multilayer recursive feature elimination method based on an embedded integer-coded genetic algorithm, MGRFE, is proposed here, which is aimed at selecting the gene combination with minimal size and maximal information. On the basis of 19 benchmark microarray datasets including multiclass and imbalanced datasets, MGRFE outperforms state-of-the-art feature selection algorithms with better cancer classification accuracy and a smaller selected gene number. MGRFE could be regarded as a promising feature selection method for high-dimensional datasets especially gene expression data. Moreover, the genes selected by MGRFE have close biological relevance to cancer phenotypes. The source code of our proposed algorithm and all the 19 datasets used in this paper are available at https://github.com/Pengeace/MGRFE-GaRFE.

18.
Theranostics ; 9(10): 2827-2842, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31244926

RESUMO

In neurosurgery, the precise diagnosis and treatment of tumor diseases are challenging to realize. Current clinical diagnoses lack fast and accurate intraoperative information. Therefore, the development of new methods and techniques to assist neurosurgeons intraoperatively is necessary. Optical diagnosis is a promising method to provide accurate information about biological tissues in a short time. Therefore, in this study, we proposed a dual-modality optical diagnostic method through point-to-face registration fusion in the optical system. We incorporated quantitative autofluorescence spectroscopy and optical coherence tomography (OCT) and evaluated our methods in an animal model. Methods: A mouse model consisting of 16 nude mice was built by injecting the mouse brains with human glioma cells. Preoperative bioluminescence imaging was used to evaluate the growth states of tumors and locate the tumor sites. Quantitative autofluorescence spectroscopy, which provided local biochemical information with single-point detection, and OCT, which provided relatively global structural information with en face mapping scanning, were combined using the point-to-face registration fusion method to provide precise diagnostic information for identifying the brain tumors. Postoperative pathology was performed to evaluate the sensitivity and specificity of optical diagnosis. Results: Ex vivo quantitative autofluorescence spectroscopy and OCT imaging were first performed in eight mice to acquire the optimal measuring parameters for tumor staging and identification. We then performed in vivo quantitative autofluorescence spectroscopy and OCT imaging. The results showed that tumor staging could be realized through quantitative autofluorescence spectroscopy, and fusion images could be used to precisely identify tumors. The autofluorescence spectral map, OCT en face map, and fused diagnostic map had average sensitivities of 91.7%, 86.1%, and 95.9% and specificities of 93.2%, 96.0%, and 88.7%, respectively, for tumor identification. Conclusion: The dual-modality optical point-to-face registration fusion method and system we proposed could provide both biochemical information and structural information. The in vivo experimental results validated that the sensitivity (95.9%) of the fused map was higher than that of either single diagnostic modality (86.1% or 91.7%). Tumor staging was realized through quantitative autofluorescence spectroscopy. The proposed method will be applicable to future intelligent theranostic systems and improve many clinical neurosurgeries.

19.
Sci Total Environ ; 682: 629-638, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31129545

RESUMO

The degradation of pharmaceuticals and personal care products (PPCPs) by using solar photolysis in the presence of free available chlorine (FAC) was investigated in simulated drinking water. The combination of free available chlorine and sunlight irradiation dramatically accelerated the degradation of all the contaminants tested through the generation of hydroxyl radicals, reactive chlorine species (RCS) and ozone. Contaminants containing electron-donating moieties degraded quickly and were preferentially degraded by RCS and/or HO oxidation. Primidone, ibuprofen and atrazine, which contain electron-withdrawing moieties, were mainly degraded by HO. Trace amounts of O3 contributed greatly to carbamazepine's degradation. Degradation of PPCPs was accelerated in oxygenated solutions. Increasing chlorine concentrations barely enhanced removal of PPCPs bearing electron-withdrawing moieties. Higher pH generally decreased the degradation rate constants along with reduced levels of HO and Cl, but diclofenac, gemfibrozil, caffeine and carbamazepine had peak degradation rate constants at pH 7-8. The cytotoxicity using Chinese hamster ovary (CHO) cell did not show significant enhancement in solar/FAC treated water. Combining chlorination with sunlight may provide a simple and energy-efficient approach for improving the removal of organic contaminants during water treatment.

20.
Clin Respir J ; 13(8): 493-498, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31102566

RESUMO

INTRODUCTION: Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease characterized by variable progression. The prealbumin (PA) is a parameter in a routine blood biochemistry examination. We sought to investigate the prognostic value in IPF patients. OBJECTIVES: To evaluate the prognosis value in patients with IPF. METHODS: Blood biochemistry examination, demographics, pulmonary function data from patients with IPF consulted in Beijing Chao-Yang Hospital and China-Japan Friendship Hospital between July 2012 and December 2016 were collected. Infection, liver and kidney dysfunction and lung transplantation are excluded from the cohort. RESULTS: The result of multivariate Cox analysis showed that PA was significant prognostic indicator of survival along with BMI, FVC, serum albumin protein and serum global protein. The patients with PA concentration <0.2 mg/L had shorter survival compared with those whose PA were normal. Although the survival had no significant difference between the patients with PA concentration < 0.2 mg/L and albumin < 35 g/L and those with PA concentration < 0.2 mg/L, the average survival time of patients with PA concentration < 0.2 mg/L and albumin < 35 g/L were shorter. CONCLUSIONS: Our study indicated that IPF patients with PA concentration < 0.2 mg/L have poorer outcome. Further studies are warranted to indentify PA as a predictor for IPF patients outcomes and explore the role of PA in the pathogenesis of IPF.

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