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1.
Environ Sci Technol ; 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36688915

RESUMO

Vivianite (Fe3(PO4)2·8H2O) crystallization has attracted increasing attention as a promising approach for removing and recovering P from wastewaters. However, FeII is susceptible to oxygen with its oxidation inevitably influencing the crystallization of vivianite. In this study, the profile of vivianite crystallization in the presence of dissolved oxygen (DO) was investigated at pHs 5-7 in a continuous stirred-tank reactor. It is found that the influence of DO on vivianite crystallization was highly pH-related. At pH 5, the low rate of FeII oxidation at all of the investigated DO of 0-5 mg/L and the low degree of vivianite supersaturation resulted in slow crystallization with the product being highly crystalline vivianite, but the P removal efficiency was only 30-40%. The removal of P from the solution was substantially more effective (to >90%) in the DO-removed reactors at pH 6 and 7, whereas the efficiencies of P removal and especially recovery decreased by 10-20% when FeII oxidation became more severe at DO concentrations >2.5 mg/L (except at pH 6 with 2.5 mg/L DO). The elevated degree of vivianite supersaturation and enhanced rate and extent of FeII oxidation at the higher pHs led to decreases in the size and homogeneity of the products. At the same pH, amorphous ferric oxyhydroxide (AFO)─the product of FeII oxidation and FeIII hydrolysis─interferes with vivianite crystallization with the induction of aggregation of crystal fines by AFO, leading to increases in the size of the obtained solids.

2.
Accid Anal Prev ; 180: 106910, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36525717

RESUMO

Unsignalized intersection collision has been one of the most dangerous accidents in the world. How to identify road hazards and predict the potential intersection collision ahead are challenging problems in traffic safety. This paper studies the feasibility of EEG metrics to forecast road hazards and presents an improved neural network model to predict intersection collision based on EEG metrics and driving behavior. It is demonstrated that EEG metrics show significant differences between collision and non-collision cases. It indicates that EEG metrics can serve as effective indicators to predict the collision probability. The drivers with higher relative power in fast frequency band (alpha and beta), lower relative power in slow frequency band (delta and theta) are more likely to have conflicts. The prediction using three machine learning models (Multi-layer perceptron (MLP), Logistic regression (LR) and Random forest (RF)) based on three input datasets (only EEG metrics, only driving behavior and combined EEG metrics with driving behavior) are compared. The results show that for single time point prediction, MLP model has the highest accuracy among three machine learning models. The model solely based on EEG metrics datasets has higher accuracy than driving behavior as well as combined datasets. However, for multi-time point prediction, the accuracy of MLP is only 73.9%, worse than LR and RF. We improved the MLP model by adding attention mechanism layer and using random forest model to select important features. As a consequence, the accuracy is greatly improved and reaches 88%. This study demonstrates the importance and feasibility of EEG signals to identify unsafe drivers ahead. The improved neural network model can be helpful to reduce intersection accidents and improve traffic safety.


Assuntos
Acidentes de Trânsito , Condução de Veículo , Humanos , Acidentes de Trânsito/prevenção & controle , Redes Neurais de Computação , Aprendizado de Máquina , Eletroencefalografia
3.
Front Med (Lausanne) ; 9: 955125, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36425108

RESUMO

Objectives: We sought to evaluate the prognostic value of blood routine parameters and biochemical parameters, especially inflammation-related biomarkers, and establish an inflammation-related prognostic model in Chinese patients with idiopathic pulmonary fibrosis (IPF). Material/methods: Patients diagnosed as IPF at Beijing Chaoyang Hospital and aged 40 years and older were consecutively enrolled from June 2000 to March 2015, and finally, a total of 377 patients were enrolled in the derivation cohort. The follow-up ended in December 2016. We used Cox proportional hazard model to calculate the hazard ratio (HR) and establish the prognostic model. The discrimination and calibration of the prognostic model were evaluated in an independent validation cohort enrolled from China-Japan Friendship Hospital between January 2015 and December 2019. Results: Multivariate analysis revealed that patients with elevated monocyte-to-red blood cell count ratio (MRR) and monocyte counts showed increased risk of mortality. The clinical-physiological-biomarker (CPB) index and CPB stage we established in this study were a significant predictor, and the C-index for CPB index and CPB stage in the validation cohort was 0.635 (95% CI: 0.558-0.712) and 0.619 (95% CI: 0.544-0.694), respectively. Patients in CPB stage III had the poorest survival. Conclusion: We developed and validated a new inflammation-related prognostic model (CPB index and CPB stage) which was integration of age, gender, FVC (%, predicted), DLCO (%, predicted), Charlson Comorbidity Index, and blood monocyte counts. This prediction model exhibited strong ability in predicting mortality in Chinese patients with IPF.

4.
Nucleic Acids Res ; 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36318250

RESUMO

DNA methylation, as the most intensively studied epigenetic mark, regulates gene expression in numerous biological processes including development, aging, and disease. With the rapid accumulation of whole-genome bisulfite sequencing data, integrating, archiving, analyzing, and visualizing those data becomes critical. Since its first publication in 2015, MethBank has been continuously updated to include more DNA methylomes across more diverse species. Here, we present MethBank 4.0 (https://ngdc.cncb.ac.cn/methbank/), which reports an increase of 309% in data volume, with 1449 single-base resolution methylomes of 23 species, covering 236 tissues/cell lines and 15 biological contexts. Value-added information, such as more rigorous quality evaluation, more standardized metadata, and comprehensive downstream annotations have been integrated in the new version. Moreover, expert-curated knowledge modules of featured differentially methylated genes associated with biological contexts and methylation analysis tools have been incorporated as new components of MethBank. In addition, MethBank 4.0 is equipped with a series of new web interfaces to browse, search, and visualize DNA methylation profiles and related information. With all these improvements, we believe the updated MethBank 4.0 will serve as a fundamental resource to provide a wide range of data services for the global research community.

5.
Clin Respir J ; 2022 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-36437511

RESUMO

INTRODUCTION: The GAP model was widely used as a simple risk "screening" method for patients with idiopathic pulmonary fibrosis (IPF). OBJECTIVES: We sought to validate the GAP model in Chinese patients with IPF to evaluate whether it can accurately predict the risk for mortality. METHODS: A total of 212 patients with IPF diagnosed at China-Japan Friendship Hospital from 2015 to 2019 were enrolled. The latest follow-up ended in September 2022. Cumulative mortality of each GAP stage was calculated and compared based on Fine-Gray models for survival, and lung transplantation was treated as a competing risk. The performance of the model was evaluated in terms of both discrimination and calibration. RESULTS: The cumulative mortality in patients with GAP stage III was significantly higher than that in those with GAP stage I or II (Gray's test p < 0.0001). The Harrell c-index for the GAP calculator was 0.736 (95% CI: 0.667-0.864). The discrimination for the GAP staging system were similar with that for the GAP calculator. The GAP model overestimated the mortality rate at 1- and 2-year in patients classified as GAP stage I (6.90% vs. 1.77% for 1-year, 14.20% vs. 6.78% for 2-year). CONCLUSIONS: Our findings indicated that the GAP model overestimated the mortality rate in mild group.

6.
J Opt Soc Am A Opt Image Sci Vis ; 39(5): 782-792, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-36215437

RESUMO

We present anl omnidirectional 3D autostereoscopic aerial display with continuous parallax. Integral photography (IP) combined with polyhedron-shaped aerial imaging plates (AIPs) is utilized to achieve an extended view angle of 3D aerial images. With optical theoretical analysis and an aerial in situ rotation design, a 3D aerial display with an enlarged viewing angle is realized. In particular, the proposed 3D aerial display can realize any assigned angle within 360 deg. We also optimize the aerial display with artifact image removal and floating image brightness analysis. Experiments are performed to prove the 3D aerial display with full-motion parallax, continuous viewpoints, and multiplayer interaction. The proposed system is an attractive prospect of non-contact interaction and multi-person collaboration.

7.
Biosensors (Basel) ; 12(9)2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36140097

RESUMO

In this paper, we report a point-of-care (POCT) testing strip based on a porous membrane structure for whole blood separation and colorimetric analysis without external supporting equipment. Conventional blood tests rely on large instruments for blood pretreatment and separation to improve measurement accuracy. Cellulose acetate (CA) membranes with different pore diameters and structures were prepared via a non-solvent method for the separation of whole blood. Among them, CA@PEG-2000 membranes with nano-pores on the surface and micro-pores in the interior facilitated the capture of blood cells on the surface, as well as the free diffusion of plasma through the porous interior structure. The fluid flow of blood in the asymmetric porous structure can be theoretically estimated using the Lucas-Washburn equation. Compared with the conventional paper strips and other porous membranes, the CA@PEG-2000 membrane with an immobilized sensing layer exhibited efficient blood separation, a short response time (less than 2 min), an ultralow dosage volume (5 µL), and high sensitivity. The fabricated blood separation membranes can be further used for the detection of various biomarkers in whole blood, providing additional options for rapid quantitative POCT tests.


Assuntos
Colorimetria , Plasma , Biomarcadores/análise , Plasma/química , Testes Imediatos , Porosidade
8.
Water Res ; 225: 119120, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-36126426

RESUMO

The triplet states of dissolved organic matter (3DOM*) have been well known to oxidize various organic contaminants, but evidence of their reducing properties are largely scarce. In this work, chlorine dioxide (ClO2) as a single-electron oxidant was used as a probe to evaluate the reduction property of 3DOM*. The reduction of ClO2 to chlorite was observed in the solutions of model photosensitizers (i.e., 4-carboxybenzophenone, benzophenone, acetophenone, 3-methoxyacetophenone, naphthalene, and xanthone) during UV irradiation with the presence of ClO2, though they are resistant to ClO2 oxidation in the dark. The reducing property of the triplet states of photosensitizers was verified and their second-order reaction rate constants with ClO2 were determined to be in the range of 1.45(± 0.03)× 109 - 2.18(± 0.06) × 109 M-1 s-1 at pH 7.0. The quenching tests excluded the role of other reactive species (e.g., HO•, O(3P), Cl•, ClO• and HOCl/OCl-, O2•- and eaq-) in ClO2 reduction to chlorite when using model photosensitizers and DOM isolates. Chlorite formation was 48.1-90.4% and 4812.8-7721.8% higher during UV irradiation with the presence of ClO2 and DOM than those without UV irradiation or without DOM present, respectively. The enhancement was attributed to the enhanced electron donating capacity (chlorite precursors) of DOM upon UV irradiation and also to 3DOM* acting as an electron donor reducing ClO2 to chlorite. This study highlighted the important role of 3DOM* as a reductant.


Assuntos
Compostos Clorados , Purificação da Água , Xantonas , Fármacos Fotossensibilizantes , Substâncias Redutoras , Compostos Clorados/química , Óxidos/química , Oxirredução , Oxidantes , Benzofenonas , Naftalenos , Acetofenonas , Cloro/química
9.
Anal Chim Acta ; 1226: 340263, 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36068062

RESUMO

The upregulation of glucose transporter (GLUT) is a typical pathological marker in numerous cancer types and a potential target for anti-cancer drug therapy. We developed a cell membrane-based glucose sensor for real-time monitoring of GLUT transport kinetics. By combining hydrogel layers and liposomes, a planar cell membrane was constructed over the electrode, preventing pore leakage and allowing for highly sensitive and selective measurements. Based on this continuous monitoring technique, we investigated the effect of GLUT1-specific inhibitors such as Cytorelaxation B and BAY-876. We also measured the affinity of different hexoses to GLUT1 using a normalized response time comparison based on the cell membrane sensor. Experimental results were consistent with the molecular docking simulation, indicating that the sensor can be adapted to measure the glucose transport kinetics in different pharmacological conditions. This work demonstrated that cell membrane transport channels could maintain their transmembrane function in-vitro, and it has potential application in evaluating drug-receptor interaction.


Assuntos
Proteínas Facilitadoras de Transporte de Glucose , Proteínas de Transporte de Monossacarídeos , Transporte Biológico , Membrana Celular/metabolismo , Glucose/metabolismo , Proteínas Facilitadoras de Transporte de Glucose/metabolismo , Transportador de Glucose Tipo 1/metabolismo , Cinética , Simulação de Acoplamento Molecular
10.
Microsc Res Tech ; 2022 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-36056697

RESUMO

In the current study, the effect of trivalent cerium (Ce3+ ) on the production of reactive oxygen species (ROS) was investigated in the root of Arabidopsis thaliana by an in vitro study. The roots of A. thaliana were exposed with 0, 1, and 5 µmol/L Ce3+ for 12 h in vitro. It was found that the level of H2 O2 , O2 .- , and ·OH was enhanced by 5 µmol/L Ce3+ , but reduced by 1 µmol/L Ce3+ . The activities of peroxidase (POD), catalase (CAT), and superoxidase dismutase (SOD) were enhanced by 1 µmol/L Ce3+ , but reduced by 5 µmol/L Ce3+ . Moreover, we used a laser-scanning confocal microscopy to detect the changes of ROS in the root cells of A. thaliana by using a fluorochrome 2',7'-dichlorofluorescein diacetate (H2 DCF-DA). It showed that the level of ROS was declined in the root cells treated by 1 µmol/L Ce3+ , but the oscillation of ROS was found in the root cells treated with 5 µmol/L Ce3+ . In addition, REEs affect the uptake of mineral elements, which may be related to the oxidative stress in the cells of roots. In all, the data of our study indicated that the appropriate concentration of Ce3+ exhibited an anti-oxidation property and improved the defense system in the root cells of A. thaliana.

11.
Annu Int Conf IEEE Eng Med Biol Soc ; 2022: 198-202, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-36086447

RESUMO

According to the 2016 World Health Organization(WHO) Classification scheme for gliomas, isocitrate dehydrogenase(IDH) is a very important basis for diagnosis. There is a strong relationship between IDH mutation status and glioma prognosis. Therefore, it is important to predict the IDH mutation status for preoperatively treating glioma. In the past decade, there has been an increase in the use of machine learning, particularly deep learning, for medical diagnosis. To date, many methods using either deep learning or radiomics have been proposed for predicting glioma IDH mutation status. In this study, we proposed an intra- and inter-modality fusion model, which first fuses both Magnetic Resonance Imaging-based (MRI-based) radiomics with deep learning features in each modality (intra-modality fusion) and then the prediction results from each modality are fused by using an inter-modality regression model, to improve the IDH status prediction accuracy. The effectiveness of the proposed method is validated via our private glioma data set from the First Affiliated Hospital of Zhengzhou University (FHZU) in Zhengzhou, China. Our proposed method is superior to current state-of-the-art methods with an accuracy of 0.77, precision of 0.77, recall of 0.77, and F1 score of 0.77, thereby exhibiting an 8% increase in accuracy to predict the IDH mutation status for glioma treatment.


Assuntos
Neoplasias Encefálicas , Glioma , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/patologia , Glioma/diagnóstico por imagem , Glioma/genética , Glioma/patologia , Humanos , Isocitrato Desidrogenase/genética , Imageamento por Ressonância Magnética/métodos , Mutação
12.
BMC Pulm Med ; 22(1): 297, 2022 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-35918677

RESUMO

BACKGROUND: Recent years, idiopathic pulmonary fibrosis (IPF) is thought to be a disease of alveoli as well as small airways. This study aimed to demonstrate the clinical feature, predictor, and prognosis of small airway dysfunction (SAD) in Chinese patients with IPF. METHODS: We enrolled 416 patients with IPF who hospitalized in Beijing Chao-Yang Hospital from 2000 to 2014 in this study, and the follow-up ended at December 2016. We collected demographic information, clinical examination results, spirometry results, HRCT results, and blood gas results during the study. Logistic regression analysis was used to identify the predictor for SAD. The COX proportional hazard model was used to analysis the prognosis effect of SAD. RESULTS: Among all the participants, 165 (39.66%) patients had SAD. FEV1 (% predicted) and FEV3/FVC were significantly associated with SAD in patients with IPF. IPF patients with lower FEV1 (% predicted, OR 30.04, 95% CI 9.61-93.90) and FEV3/FVC (OR 77.76, 95% CI 15.44-391.63) had increased risk for SAD. Patients with SAD were associated with significantly increased risk of mortality in patients with IPF (HR 1.73, 95% CI 1.02-2.92), as well as in IPF patients without other pulmonary comorbidities (COPD, emphysema, and asthma). CONCLUSIONS: Spirometry-defined SAD was like 40% in patients with IPF. Lower FEV1 (% predicted) and FEV3/FVC were main predictors for SAD. IPF patients with SAD showed poorer prognosis.


Assuntos
Fibrose Pulmonar Idiopática , Enfisema Pulmonar , China , Humanos , Pulmão/diagnóstico por imagem , Modelos de Riscos Proporcionais , Estudos Retrospectivos
13.
ACS Appl Mater Interfaces ; 14(35): 39681-39700, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36006298

RESUMO

Wearable electronics with development trends such as miniaturization, multifunction, and smart integration have become an important part of the Internet of Things (IoT) and have penetrated various sectors of modern society. To meet the increasing demands of wearable electronics in terms of deformability and conformability, many efforts have been devoted to overcoming the nonstretchable and poor conformal properties of traditional functional materials and endowing devices with outstanding mechanical properties. One of the promising approaches is composite engineering in which traditional functional materials are incorporated into the various polymer matrices to develop different kinds of functional composites and construct different functions of stretchable electronics. Herein, we focus on the approach of composite engineering and the polymer matrix of silicone rubber (SR), and we summarize the state-of-the-art details of silicone rubber-based conductive composites (SRCCs), including a summary of their conductivity mechanisms and synthesis methods and SRCC applications for stretchable electronics. For conductivity mechanisms, two conductivity mechanisms of SRCC are emphasized: percolation theory and the quantum tunneling mechanism. For synthesis methods of SRCCs, four typical approaches to synthesize different kinds of SRCCs are investigated: mixing/blending, infiltration, ion implantation, and in situ formation. For SRCC applications, different functions of stretchable electronics based on SRCCs for interconnecting, sensing, powering, actuating, and transmitting are summarized, including stretchable interconnects, sensors, nanogenerators, antennas, and transistors. These functions reveal the feasibility of constructing a stretchable all-in-one self-powered microsystem based on SRCC-based stretchable electronics. As a prospect, this microsystem is expected to integrate the functional sensing modulus, the energy harvesting modulus, and the process and response modulus together to sense and respond to environmental stimulations and human physiological signals.

14.
Water Res ; 223: 118967, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35973248

RESUMO

Dissolved organic matter (DOM), ubiquitous in natural waters, is known to inhibit the degradation of micropollutants in the advanced oxidation processes such as the UV/peroxydisulfate process. However, the quantitative understanding of the inhibitory pathways is missing. In this study, guanosine, aniline and catechol belonging to amines, purines and phenols were first investigated due to their resistance to UV irradiation at 254 nm and similar reactivity with SO4•- and HO•, respectively. The presence of 0.5 mgC L-1 Suwannee River NOM (SRNOM) inhibited their degradation rates by 72.9%, 54.5%, and 32.4%, respectively, despite their similar degradation rates in the absence of SRNOM. The results highlight the importance of reverse reduction of oxidation intermediates to the parent compound by antioxidant moieties in SRNOM besides the inner filtering and radical scavenging effects. The three inhibitory pathways were quantified for 34 common micropollutants. In the presence of 0.5 mgC L-1 SRNOM, inner filtering effect was found to contribute less than 2.8% of the inhibitory percentages (IP). Radical scavenging effects contribute between 10.7% and 38.9% and compounds having lower reactivity with SO4•- (< 4.0 × 109 M-1 s-1) tended to be inhibited more strongly. The IP of reverse reduction effects of SRNOM varied significantly from none up to 70.8%. It was linearly related with a micropollutant's reduction potential. Purines and amines generally exhibited more pronounced reverse reduction inhibition than phenols. The results of this study provide guidance on improving the elimination efficiency of micropollutants.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Compostos de Anilina , Antioxidantes , Catecóis , Matéria Orgânica Dissolvida , Guanosina , Cinética , Oxirredução , Fenóis , Purinas , Raios Ultravioleta , Poluentes Químicos da Água/análise , Purificação da Água/métodos
15.
iScience ; 25(8): 104680, 2022 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-35942087

RESUMO

Hadal ocean biosphere, that is, the deepest part of the world's oceans, harbors a unique microbial community, suggesting a potential uncovered co-occurring virioplankton assemblage. Herein, we reveal the unique virioplankton assemblages of the Challenger Deep, comprising 95,813 non-redundant viral contigs from the surface to the hadal zone. Almost all of the dominant viral contigs in the hadal zone were unclassified, potentially related to Alteromonadales and Oceanospirillales. 2,586 viral auxiliary metabolic genes from 132 different KEGG orthologous groups were mainly related to the carbon, nitrogen, sulfur, and arsenic metabolism. Lysogenic viral production and integrase genes were augmented in the hadal zone, suggesting the prevalence of viral lysogenic life strategy. Abundant rve genes in the hadal zone, which function as transposase in the caudoviruses, further suggest the prevalence of viral-mediated horizontal gene transfer. This study provides fundamental insights into the virioplankton assemblages of the hadal zone, reinforcing the necessity of incorporating virioplankton into the hadal biogeochemical cycles.

16.
Anal Chim Acta ; 1221: 340168, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35934387

RESUMO

Targeting the long-term monitoring of biological carbohydrate metabolism, we developed a one-step screen-printing method to fabricate electrochemical sensors using an enzyme microparticle hybrid ink. Most enzymes have low stability in high temperatures and organic solvents, making conventional enzyme modification a bottom-up procedure to be performed after electrode fabrication, resulting in inactivation and detachment in long-term work. Enzyme-loaded microparticles prepared by manganese carbonate co-precipitation had higher stability than free enzymes, which could to be mixed directly with carbon paste for direct screen-printing. Due to the co-printing immobilization and the local hydration environment in enzyme particles, the prepared electrodes exhibited higher long-term operational stability than the conventional multi-step cross-linking method. In the sensing applications, we prepared microparticles loaded with single enzyme (glucose oxidase) and dual enzymes (ß-galactosidase and glucose oxidase) for glucose and lactose monitoring, respectively. Both electrodes can accurately measure the consumption of the corresponding carbohydrates throughout the cell or bacterial culture period thus providing a sensing platform for bio-metabolic monitoring and drug screening.


Assuntos
Técnicas Biossensoriais , Glucose Oxidase , Técnicas Biossensoriais/métodos , Eletrodos , Glucose , Glucose Oxidase/metabolismo , Tinta
17.
IEEE J Biomed Health Inform ; 26(11): 5450-5460, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35947566

RESUMO

The pattern recognition (PR) based on surface electromyography (sEMG) could improve the quality of daily life of amputees. However, the lack of robustness and adaptability hinders its practical application. To realize the long-term reliability and user adaptability simultaneously, a novel multi-task dual-stream supervised domain adaptation (MDSDA) network based on convolutional neural network (CNN) was proposed. A long-term multi-subject sEMG signal acquisition was conducted to validate the performance of MDSDA, recruiting 12 able-bodied subjects. A total of thirty gestures were used for the acquisition, including one set of static gestures and two sets of dynamic gestures. The long-term multi-subject sEMG dataset is publicly available at the website. Four train-test estimations were designed to evaluate the robustness and adaptability of MDSDA. The results showed that MDSDA outperformed CNN and fune-tuning. Furthermore, we studied the divisibility between static and dynamic gestures that performed similar actions. The outcomes demonstrated that there existed high separability between them. This may be helpful to reduce the signal collection burden. Experimental results proved MDSDA has the potential to provide a robust and generalized PR system for the clinic applications.


Assuntos
Gestos , Reconhecimento Automatizado de Padrão , Humanos , Eletromiografia/métodos , Reprodutibilidade dos Testes , Redes Neurais de Computação , Mãos , Algoritmos
18.
Cancer Med ; 2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36028993

RESUMO

BACKGROUNDS: We aimed to test whether the prediction of presurgical metabolic syndrome for postsurgical survival outcomes of gastric cancer hinges upon cigarette smoking status. METHODS: This study is a part of the ongoing Fujian prospective investigation of cancer (FIESTA) study. Patients with gastric cancer received radical resection of primary gastric cancer between January 2000 and December 2010, with the latest follow-up ended in December 2015. The 1:1 propensity score matching analysis was adopted to balance confounders between smokers and never-smokers. Effect-size estimates are expressed as hazard ratio (HR) with 95% confidence interval (CI). Model performance was evaluated using the Hosmer and Lemeshow test and 10-fold cross-validated area under the receiver operating characteristic curve (AUROC). Statistical analyses were completed with SAS software (v9.4). RESULTS: Total 2779 patients with gastric cancer were analyzed, including 2223 smokers and 556 never-smokers. Median follow-up time was 45.6 months. Cigarette smoking was not associated with postsurgical survival differences. Presurgical metabolic syndrome complication was significantly associated with increased gastric cancer-specific mortality in smokers (HR [95% CI]: 2.73 [1.53-4.89], p < 0.001), but not in never-smokers. Relative excess risk due to interaction was estimated to be 2.43 (95% CI: 0.40-4.45). After constructing a risk assessment score, one unit increment was associated with 10% reduced risk of gastric cancer-specific mortality (HR [95% CI]: 0.90 [0.88-0.91], p < 0.001), with 10-fold cross-validated AUROC being 0.82 (95% CI: 0.74-0.92). CONCLUSIONS: Our findings showed that the prediction of presurgical metabolic syndrome for gastric cancer-specific mortality was more evident in smokers. Practically, this study provides evidence base for future personalized prediction and helped risk-stratify gastric cancer patients who might experience serious postsurgical consequences.

19.
J Cell Physiol ; 237(9): 3661-3670, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-35853150

RESUMO

AZD1208, a pan-inhibitor that can effectively inhibit PIM kinase, is used for the treatment of advanced solid tumors and malignant lymphomas. Numerous studies have proved its curative effects while its potential cellular toxicity on reproduction was still little known. In this study, we investigated the toxic effects of AZD1208 on mouse oocytes. The results showed that AZD1208 treatment did not affect meiotic resumption, but postponed oocyte maturation as indicated by delayed first polar body extrusion. Further mechanistic study showed that AZD1208 treatment delayed spindle assembly. In addition, we found that oocytes treated with AZD1208 showed mitochondrial dysfunction. Abnormal mitochondrial clusters with decreased mitochondrial membrane potential were observed in oocytes during incubation in vitro. Moreover, increased oxidative stress was observed by testing the level of reactive oxygen species. In summary, our results suggest that AZD1208 treatment influences oocyte meiotic progression by causing mitochondrial dysfunctions and subsequent delayed spindle assembly.


Assuntos
Compostos de Bifenilo , Oócitos , Animais , Compostos de Bifenilo/farmacologia , Meiose , Camundongos , Mitocôndrias , Oócitos/metabolismo , Tiazolidinas/metabolismo
20.
Phytochem Anal ; 33(6): 879-885, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35668032

RESUMO

OBJECTIVES: Acanthopanax senticosus (Rupr. & Maxim.) Harms is a medicinal and edible plant which is clinically used for the recovery and treatment of cardiovascular and central diseases. As a characteristic active pharmaceutical ingredient of Acanthopanax senticosus, eleutheroside E is the core of the therapeutic effect. Organic solvent extraction has low selectivity, low extraction rate, difficulty in separation and purification and safety risks. The purpose of this study was to extract the effective component of Acanthopanax senticosus with a new green solvent. METHODS: In this article, two kinds of deep eutectic solvents (DESs) (DES-1 and DES-2) were synthesised by heating and stirring methods. Eleutheroside E was extracted by ultrasonic extraction with two kinds of DES as extractants and quantitatively analysed by Orbitrap-tandem mass spectrometry (MS/MS). RESULTS: The main results showed that the initial polarity of the DES was similar to that of 60 to 80% ethanol and hydrogen bond donors were the main factors affecting the polarity of DES. In the test, the viscosity of DES was higher than that of ethanol, and even the addition of a small amount of water (10%) caused intermolecular hydrogen bond disruption and redistribution of the solvent, resulting in a significant decrease in solvent viscosity. The solvents in the test group were stable after standing at 5°C in the dark for 100 days. The extraction rate of eleutheroside E by DES solvent was 5-6 times higher than that by ethanol. DES-1 and DES-2 can efficiently extract eleutheroside E. CONCLUSION: This study developed a new method for the application of the green extraction of eleutheroside E with certain practical significance.


Assuntos
Eleutherococcus , Eleutherococcus/química , Etanol , Glucosídeos , Lignanas , Solventes , Espectrometria de Massas em Tandem , Tecnologia
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