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1.
J Alzheimers Dis ; 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31594229

RESUMO

Recent studies found that poor oral hygiene was associated with increased risk of dementia, and the number of oral bacteria significantly increased in the brain tissues of patients with Alzheimer's disease (AD), suggesting that the oral microbiota may play an important role in the pathogenesis of AD. However, the actual composition of oral bacteria communities in patients with AD and whether these oral bacteria are associated with disease severity remain largely unknown. Also, the APOEɛ4 polymorphism is a strong risk factor for sporadic AD, and it would be pertinent to see if the bacterial flora was different in those patients who were APOEɛ4 positive. A total of 78 subjects were recruited in this study, including 39 patients with AD and 39 healthy controls. Saliva was collected from each subject. 16S ribosomal RNA (16S rRNA) sequencing was conducted to analyze the salivary microbiota, and Sanger sequencing was performed to analyze the APOE genotype. There was a significantly lower richness and diversity of saliva microbiota detected in AD patients than healthy controls. The relative abundance of Moraxella, Leptotrichia, and Sphaerochaeta in the saliva of AD patients greatly increased, whereas that of Rothia was significantly reduced. Compared with APOEɛ4 (-) patients, the level of Abiotrophia and Desulfomicrobium was comparatively abundant, while Actinobacillus and Actinomyces decreased significantly in patients carrying the APOEɛ4. No bacteria were found to be associated with the severity of AD. This is the first study to analyze the salivary microorganisms in patients with AD, and we discovered that the composition of salivary microbiome was altered in AD, providing further support for the role of the oral microbiome in AD development.

2.
Int J Mol Med ; 2019 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-31573042

RESUMO

Diallyl disulfide (DADS) is one of the primary components of garlic and it exhibits a broad range of biological activities. In the present study, the effects of DADS on lipid metabolism and its potential role in the modulation of the gut microbiome were determined. Hematoxylin and eosin and oil­red O staining were used to assess the liver and intestinal tissues of mice treated with DADS. The expression of lipid metabolism­associated genes was measured using reverse transcription­quantitative PCR (RT­qPCR). The effects of DADS on the gut microbiome were measured using 16S recombinant (r)DNA gene analysis. The results revealed that the serum non­esterified free fatty acids, high density lipoprotein­cholesterol, low density lipoprotein­cholesterol, serum total cholesterol, liver triglyceride and total cholesterol levels of the mice fed with a low­dose of DADS was significantly higher when compared with the control. Hematoxylin and eosin and oil­red O staining demonstrated that DADS induced fatty liver in mice. The results of the RT­qPCR revealed that the expression levels of a number of lipid metabolism­associated genes were altered in the livers of mice treated with DADS. The 16S rDNA gene analysis demonstrated that the mice fed on a normal diet treated with a low­dose of DADS had decreased levels of bacteria from the Bacteroidetes phyla and increased levels of bacteria from the Firmicutes phyla. The Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed the top 20 pathways enriched in the low­dose DADS group of mice fed with a normal diet. In the present study, low­dose DADS induced fatty liver and altered the gut microbiota, similar to the phenotype induced by a high fat diet, by regulating the expression of lipid metabolism associated genes.

3.
Medicine (Baltimore) ; 98(39): e17167, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31574823

RESUMO

BACKGROUND: The association between plasma interleukin-6 (IL-6) levels and the development of febrile seizures (FS) has been reported in multiple previous studies, which showed significantly higher serum IL-6 levels in FS patients than in control patients. However, the mechanism underlying this association remains unclear. One previous study indicated an increased frequency of the -174 GG and -597 GG genotypes in FS patients. Although IL-6 gene polymorphisms may be associated with FS risk, this association remains a matter of debate. OBJECTIVE: Considering the lack of meta-analyses addressing the possible association between IL-6 gene polymorphisms and the risk of FS, we aimed to perform a meta-analysis to determine the association of IL-6 gene polymorphisms (-572, -174, -597) with the risk of FS. METHODS: We conducted a systematic literature search in the PubMed, EMBASE, and WANFANG databases to collect eligible articles. The associations of IL-6 gene polymorphisms with FS risk were evaluated by calculating the pooled odds ratios and 95% confidence intervals. The dominant, recessive, heterozygous, homozygous, and allele genetic models were used to calculate the combined ORs. RESULTS: Our meta-analysis showed that IL-6 (-572, -174, -597) polymorphisms were significantly associated with susceptibility to FS. CONCLUSION: This study provided knowledge regarding the association of IL-6 (572, 174, 597) polymorphisms with susceptibility to FS. The T allele and TT genotype may be associated with an increased risk for FS.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31587466

RESUMO

Electroplating technique has been studied for centuries not only in laboratory but also in industry, satisfying requirements for machinery, electronics, automobile, aviation and other fields. Lithium metal anode is the Holy Grail electrode for its high energy density. But the recyclability of lithium metal batteries still remains quite challenging to be addressed. Considering the essence for both conventional electroplating and lithium plating is the metal cations reduction, we believe some mature industrial knowledge for electroplating technique can be applied to revisit the electroplating process for lithium metal anodes due to the same electrochemical principles. In conventional electroplating, some strategies like using additives, modifying substrates, applying pulse current and agitating electrolyte have been explored to suppress dendrite growth. These methods are also demonstrated effectively in lithium metal anode. Inspired by that, we revisit the fundamental electroplating theory for lithium metal anodes in this Minireview, mainly drawing attention to the theory of electroplating thermodynamics and kinetics. Analysis of essential difference between traditional electroplating and plating/stripping of lithium metal anodes is also presented. We hope the basic electroplating theory can help to expedite the lithium metal batteries implemented into market and the study of lithium plating/stripping can further enrich the knowledge of electroplating technique.

5.
J Vis Exp ; (150)2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31475963

RESUMO

Flavonols are a major subclass of flavonoids with a variety of biological and pharmacological activities. Here, we provide a method for the in vitro enzymatic synthesis of a flavonol. In this method, Atf3h and Atfls1, two key genes in the biosynthetic pathway of the flavonols, are cloned and overexpressed in Escherichia coli. The recombinant enzymes are purified via an affinity column and then a bienzymatic cascade is established in a specific synthetic buffer. Two flavonols are synthesized in this system as examples and determined by TLC and HPLC/LC/MS analyses. The method displays obvious advantages in the derivation of flavonols over other approaches. It is time- and labor-saving and highly cost-effective. The reaction is easy to be accurately controlled and thus scaled up for mass production. The target product can be purified easily due to the simple components in the system. However, this system is usually restricted to the production of a flavonol from a flavanone.

6.
Opt Express ; 27(14): 19852-19863, 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31503741

RESUMO

Surface plasmon resonance (SPR) enables strong field confinement, opening thereby new avenues for device miniaturization and reducing energy consumption. Plasmonic devices with electrical tunability attract tremendous interest for various applications. Most of the current researches achieved SPR modulation with relatively large driving voltages, or by other relatively low-speed tuning approaches, such as thermo-optic, magneto-optic, acousto-optic etc. In this paper, we propose and demonstrate an efficiently electrical SPR modulation based on lithium niobate (LN) with gold nanolayer (~81 nm) via electron-plasmon interaction. Efficient intensity modulation and wavelength shift (in visible band) of ~5.7 dB/V and ~36.3 nm/V are respectively obtained with low DC current. More importantly, modulation phenomenon of field distribution dependent is also observed and experimentally unveiled. Further performance is analyzed in terms of AC modulation and polarization characteristics. This key achievement opens up opportunities for applications such as optical interconnection, electric field sensing, electrically plasmonic modulation, etc.

7.
J Diabetes Investig ; 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31529619

RESUMO

AIMS/INTRODUCTION: Asprosin is a novel secreted adipokine that is induced by fasting and promotes hepatic glucose release. In healthy humans, circulating asprosin shows circadian oscillation with an acute drop coinciding with the onset of eating. The present study investigated whether this circadian oscillation still exists in patients with type 2 diabetes mellitus. MATERIALS AND METHODS: We recruited 60 patients with type 2 diabetes mellitus and 60 individuals with normal glucose tolerance (NGT). All participants completed a 75-g oral glucose tolerance test. Fasting and 2-h postprandial serum asprosin concentrations were measured by the enzyme-linked immunosorbent assay method. Partial correlation coefficients were calculated to analyze the relationships between serum asprosin level and parameters of glucose metabolism. Multiple logistic regression analysis was used to determine the association of serum asprosin level with diabetes. RESULTS: Both fasting and postprandial asprosin levels were significantly higher in patients with type 2 diabetes mellitus. The postprandial asprosin level was apparently lower than fasting asprosin level in individuals with NGT. The fasting asprosin level closely correlated with type 2 diabetes mellitus after multiple adjustment (odds ratio 2.329, P = 0.023). Asprosin correlated negatively with change in blood glucose (r = -0.502, P < 0.001) and change in C-peptide (r = -0.467, P < 0.001) in individuals with NGT, but not in type 2 diabetes mellitus patients. CONCLUSIONS: Serum asprosin level decreased coinciding with the onset of the oral glucose tolerance test in individuals with NGT, whereas this circadian oscillation was disturbed in type 2 diabetes mellitus patients. The impaired response of asprosin to glucose fluctuation in type 2 diabetes mellitus patients might be one of the reasons for the onset of type 2 diabetes mellitus.

9.
ACS Appl Mater Interfaces ; 11(34): 30756-30762, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31386339

RESUMO

Rhombohedral GeTe can be approximated as the directional distortion of the cubic GeTe along [111]. Such a symmetry-breaking of the crystal structure results in an opposite arrangement in energy of the L and Σ valence bands, and a split of them into 3L+1Z and 6Σ+6η, respectively. This enables a manipulation of the overall band degeneracy for thermoelectric enhancements through a precise control of the degree of crystal structure deviating from a cubic structure for the alignment of the split bands. Here, we show the effect of AgBiSe2-alloying on the crystal structure as well as thermoelectric transport properties of rhombohedral GeTe. AgBiSe2-alloying is found to not only finely manipulate the crystal structure for band convergence and thereby an increased band degeneracy, but also flatten the valence band for an increased band effective mass. Both of them result in an increased density of state effective mass and therefore an enhanced Seebeck coefficient along with a decreased mobility. Moreover, a remarkably reduced lattice thermal conductivity of ∼0.4 W/m-K is obtained due to the introduced additional point defect phonon scattering and bond softening by the alloying. With the help of Bi-doping at the Ge site for further optimizing the carrier concentration, thermoelectric figure of merit, zT, of ∼1.7 and average zTave of ∼0.9 are achieved in 5% AgBiSe2-alloyed rhombohedral GeTe, which demonstrates this material as a promising candidate for low-temperature thermoelectric applications.

10.
Theranostics ; 9(16): 4663-4677, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31367248

RESUMO

Background: Microtissues constructed with hydrogels promote cell expansion and specific differentiation by mimicking the microarchitecture of native tissues. However, the suboptimal mechanical property and osteogenic activity of microtissues fabricated by natural polymers need further improvement for bone reconstruction application. Core-shell designed structures are composed of an inner core part and an outer part shell, combining the characteristics of different materials, which improve the mechanical property of microtissues. Methods: A micro-stencil array chip was used to fabricate an open porous core-shell micro-scaffold consisting of gelatin as shell and demineralized bone matrix particles modified with bone morphogenetic protein-2 (BMP-2) as core. Single gelatin micro-scaffold was fabricated as a control. Rat bone marrow mesenchymal stem cells (BMSCs) were seeded on the micro-scaffolds, after which they were dynamic cultured and osteo-induced in mini-capsule bioreactors to fabricate microtissues. The physical characteristics, biocompatibility, osteo-inducing and controlled release ability of the core-shell microtissue were evaluated in vitro respectively. Then microtissues were tested in vivo via ectopic implantation and orthotopic bone implantation in rat model. Results: The Young's modulus of core-shell micro-scaffold was nearly triple that of gelatin micro-scaffold, which means the core-shell micro-scaffolds have better mechanical property. BMSCs rapidly proliferated and retained the highest viability on core-shell microtissues. The improved osteogenic potential of core-shell microtissues was evidenced by the increased calcification based on von kossa staining and osteo-relative gene expression. At 3months after transplantation, core-shell microtissue group formed the highest number of mineralized tissues in rat ectopic subcutaneous model, and displayed the largest amount of new bony tissue deposition in rat orthotopic cranial defect. Conclusion: The novel core-shell microtissue construction strategy developed may become a promising cell delivery platform for bone regeneration.

11.
ACS Appl Mater Interfaces ; 11(35): 32441-32448, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31385690

RESUMO

Conductive hydrogels have shown great potential applications in a wide variety of fields, including artificial intelligence devices and biomedical engineering. However, it still remains a great challenge to develop a facile and cost-effective approach to achieve a conductive hydrogel with favorable qualities. Herein, we have changed the traditional ingredient of poly(vinyl alcohol) (PVA) hydrogel by the addition of phytic acid (PA), which could yield a conductive hydrogel through one freeze-thaw cycle. The PVA-PA hydrogel holds several virtues including a large stretchability (about 1100% strain), excellent conductivity (1.34 kΩ cm), and high optical transparence (about 95%). By assembling the PVA-PA hydrogel into a wearable strain sensor, the gel-based sensor has shown good performance for the real-time monitoring of human daily activities and health conditions. Moreover, one formula of the PVA-PA sol ink could rapidly convert to the gel state just by being injected on a flexible substrate under an ice-bath, which would satisfy the demand of casual writing circuits. This one-step preparation method of the PVA-PA hydrogel may open an innovative avenue for the fabrication of easy-molding and functional hydrogels with only two components under mild ambient conditions.

12.
Acta Biochim Biophys Sin (Shanghai) ; 51(9): 890-899, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31384916

RESUMO

Chlorpyrifos (CPF) is a widely used insecticide in pest control, and it can affect aquatic animals by contaminating the water. In this study, larval zebrafish were exposed to CPF at concentrations of 30, 100 and 300 µg/l for 7 days. In the CPF-treated group, lipid droplet accumulation was reduced in larval zebrafish. The levels of triglyceride (TG), total cholesterol (TC), and pyruvate were also decreased after CPF exposure. Cellular apoptosis were significantly increased in the heart tissue after CPF exposure compared with the control. Transcription changes in cardiovascular genes were also observed. Through transcriptome analysis, we found that the transcription of 465 genes changed significantly, with 398 upregulated and 67 downregulated in the CPF-treated group, indicating that CPF exposure altered the transcription of genes. Among these altered genes, a number of genes were closely related to the glucose and lipid metabolism pathways. Furthermore, we also confirmed that the transcription of genes related to fatty acid synthesis, TC synthesis, and lipogenesis were significantly decreased in larval zebrafish after exposure to CPF. These results indicated that CPF exposure induced lipid metabolism disorders associated with cardiovascular toxicity in larval zebrafish.

13.
Chem Commun (Camb) ; 55(65): 9653-9656, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31342021

RESUMO

The fast and accurate real-time monitoring of hydrogen peroxide (H2O2) secreted from living cells plays a critical role in clinical diagnosis and management. Herein, we report low-cost and self-supported MoS2 nanosheet arrays for non-enzymatic eletrochemical H2O2 detection. Under the optimal test conditions, such MoS2 electrodes exhibit extremely promising electrocatalytic performance with a low detection limit of 1.0 µM (S/N = 3) and an excellent sensitivity of 5.3 mA mM-1 cm-2. Furthermore, the detection of the trace amount of H2O2 secreted from live A549 cancer cells was successfully performed with this biosensor.


Assuntos
Dissulfetos/química , Peróxido de Hidrogênio/análise , Molibdênio/química , Nanoestruturas/química , Células A549 , Técnicas Biossensoriais/métodos , Carbono/química , Dissulfetos/síntese química , Técnicas Eletroquímicas/métodos , Humanos , Limite de Detecção
14.
Adv Mater ; 31(36): e1903387, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31276253

RESUMO

Advancing thermoelectric n-type Mg3 Sb2 alloys requires both high carrier concentration offered by effective doping and high carrier mobility enabled by large grains. Existing research usually involves chalcogen doping on the anion sites, and the resultant carrier concentration reaches ≈3 × 1019 cm-3 or below. This is much lower than the optimum theoretically predicted, which suggets that further improvements will be possible once a highly efficient dopant is found. Yttrium, a trivalent dopant, is shown to enable carrier concentrations up to and above ≈1 × 1020 cm-3 when it is doped on the cation site. Such carrier concentration allows for in-depth understand of the electronic transport properties over a broad range of carrier concentrations, based on a single parabolic band approximation. As well as reasonably high carrier mobility in coarse-grain materials sintered by hot deforming and fusing of large pieces of ingots synthesized by melting, higher thermoelectric performance than earlier experimentally reported for n-type Mg3 Sb2 is found. In particular, the thermoelectric figure of merit, zT, is even higher than that of any known n-type thermoelectric, including Bi2 Te3 alloys, within 300-500 K. This might pave the way for Mg3 Sb2 alloys to become a realistic material for n-type thermoelectrics for sustainable applications.

15.
Int J Mol Med ; 44(3): 847-856, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31257467

RESUMO

Oxidized low­density lipoprotein (ox­LDL)­mediated endothelial cell injury has an important role in the vascular complications of type 2 diabetes. Astragaloside IV (ASV) is an active component of Radix Astragali, which has been demonstrated to exert protective effects against endothelial damage. The present study explored whether microRNAs (miRNAs) are involved in mediating the protective effects of ASV on ox­LDL­induced damage in human umbilical vein endothelial cells (HUVECs). RNA sequencing and reverse transcription­quantitative PCR analyses revealed that ox­LDL treatment significantly downregulated miR­140­3p expression in HUVECs. miR­140­3p overexpression promoted cell proliferation and inhibited apoptosis in ox­LDL­induced HUVECs. However, inhibition of miR­140­3p expression could reverse the effects of ASV on ox­LDL­induced HUVECs and reactivate ASV­inhibited PI3K/Akt signaling in ox­LDL­induced HUVECs. In addition, Krüppel­like factor 4 (KLF4) was identified as a target of miR­140­3p in ox­LDL­treated HUVECs. Subsequent experiments revealed that KLF4 overexpression partially counteracted the protective effects of miR­140­3p or ASV treatment in ox­LDL­induced HUVECs. Taken together, the current findings demonstrated that the protective effects of ASV on HUVECs were dependent on miR­140­3p upregulation and subsequent inhibition of KLF4 expression, which in turn suppressed the PI3K/Akt signaling pathway. The present results shed light to the molecular mechanism by which ASV alleviated ox­LDL­induced endothelial cell damage.

16.
Bioorg Chem ; 90: 103101, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31291611

RESUMO

In this study, forty-nine kinds of traditional Chinese medicines (TCMs) were evaluated for their inhibitory activities against human carboxylesterase 2 (HCE 2) using a human liver microsome (HLM) system. Swertia bimaculata showed significant inhibition on HCE 2 at 10 µg/mL among forty-nine kinds of TCMs. The extract of Swertia bimaculata was separated by preparative HPLC to afford demethylbellidifolin (1) identified by MS, 1H NMR, and 13C NMR spectra. Demethylbellidifolin (1) was assayed for its inhibitory HCE 2 effect by HCE 2-mediated DDAB hydrolysis, and its potential IC50 value was 3.12 ±â€¯0.64 µM. Demethylbellidifolin (1) was assigned as a mixed-type competitive inhibitor with the inhibiton constant Ki value of 6.87 µM by Lineweaver-Burk and slope plots. Living cell imaging was conducted to corroborate its inhibitory HCE 2 activity. Molecular docking indicated potential interactions of demethylbellidifolin (1) with HCE 2 through two hydrogen bonds of the C-3 and C-5 hydroxy groups with amino acid residues Glu227 and Ser228 in the catalytic cavity, respectively.

17.
Medicine (Baltimore) ; 98(26): e16203, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31261566

RESUMO

RATIONALE: Essential thrombocytosis (ET) is a myeloproliferative neoplasm characterized by clonal proliferation of the megakaryocytic lineage within the bone marrow and phenotypically by an elevated platelet count in peripheral blood. Common vascular complications include thrombosis, microvascular disturbances, and hemorrhage. ET with recurrent spontaneous abortion as the primary symptom is rare. PATIENT CONCERNS: A 30-year-old pregnant woman (gestational age: 8 weeks) with a history of recurrent spontaneous abortion in the mid trimester was admitted to our hospital for further management. DIAGNOSIS: The diagnosis of ET was made based on the platelet count, bone marrow biopsy, and molecular biology testing. INTERVENTIONS: The patient was treated with interferon, heparin, and aspirin. OUTCOMES: The infant was delivered by cesarean section without complication at 28 weeks gestation due to placental abruption. The child remained healthy with no developmental abnormalities during follow-up for 2 years. LESSONS: Recurrent spontaneous abortion in the mid trimester might be associated with ET. Thus, a detailed investigation including blood routine examination to identify an abnormal platelet count is warranted for pregnant patients with such a history in order to facilitate timely treatment.


Assuntos
Aborto Espontâneo/diagnóstico , Trombocitemia Essencial/diagnóstico , Aborto Espontâneo/genética , Aborto Espontâneo/patologia , Adulto , Medula Óssea/patologia , Cesárea , Diagnóstico Diferencial , Feminino , Humanos , Gravidez , Primeiro Trimestre da Gravidez , Trombocitemia Essencial/genética , Trombocitemia Essencial/patologia , Trombocitemia Essencial/terapia
18.
Medicine (Baltimore) ; 98(26): e16209, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31261571

RESUMO

It is not clear whether age has any influence on the outcomes for sperm used for assisted reproductive technology in cryptozoospermic men. We evaluated intracytoplasmic sperm injection (ICSI) outcomes using ejaculated or testicular sperm in men with cryptozoospermia from different paternal age ranges.We conducted a retrospective observational study of 35 men with cryptozoospermia who underwent ICSI from 2010 to 2018. They were classified into 2 groups based on male age, namely < 35 years and ≥ 35 years. Each group was further divided into 2 subgroups according to the origin of sperm (ejaculated or testicular).In the <35 years group, the normal fertilization and high-quality embryo rates for ejaculated sperm were significantly higher than with testicular sperm (74.7% vs. 62.4%, P = .02; 50.5% vs. 36.6%, P = .03, respectively). However, in the ≥35 years group, the high-quality embryo and clinical pregnancy rates were significantly lower in the ejaculated sperm subgroup than in the testicular sperm subgroup (26.2% vs. 63%, P = .002; 12.5% vs. 71.4%, P = .04, respectively).This study indicates that ICSI should be performed as soon as possible for men with cryptozoospermia. When the paternal age ≥35 years, testicular sperm should be used for ICSI, as this offers better high-quality embryo and clinical pregnancy rates.


Assuntos
Oligospermia , Idade Paterna , Injeções de Esperma Intracitoplásmicas , Adulto , Ejaculação , Feminino , Humanos , Masculino , Gravidez , Taxa de Gravidez , Estudos Retrospectivos , Recuperação Espermática , Testículo
19.
Cell Rep ; 28(4): 966-978.e4, 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31340157

RESUMO

Platelet-derived growth factor receptor (PDGFR) signaling is involved in proliferation and survival in a wide array of cell types. The role of PDGFR signaling in heart regeneration is still unknown. We find that PDGFR-ß signaling decreases in myocardium with age and that conditional activation PDGFR-ß in cardiomyocytes promotes heart regeneration. Employing RNA sequencing, we show that the enhancer of zeste homolog 2 (Ezh2) can be upregulated by PDGFR-ß signaling in primary cardiomyocytes. Conditional knockout of Ezh2 blocks cardiomyocyte proliferation and H3K27me3 modification during neonatal heart regeneration with Ink4a/Arf upregulation, even in mice with myocyte-specific conditional activation of PDGFR-ß. We also show that PDGFR-ß controls EZH2 expression via the phosphatidylinositol 3-kinase (PI3K)/p-Akt pathway in cardiomyocytes. Gene therapy with adeno-associated virus serotype 9 (AAV9) encoding activated PDGFR-ß enhances adult heart regeneration and systolic function. Our data demonstrate that the PDGFR-ß/EZH2 pathway is critical for promoting cardiomyocyte proliferation and heart regeneration, providing a potential target for cardiac repair.

20.
Medicine (Baltimore) ; 98(24): e16037, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31192962

RESUMO

BACKGROUND: The association between Methylenetetrahydrofolate Reductase (MTHFR) polymorphisms and lung cancer risk in Chinese people has been widely explored; however, the results remain controversial. Thus, we conducted a meta-analysis to investigate the association between MTHFR gene polymorphisms and susceptibility to lung cancer in Chinese people. OBJECTIVE: We performed an updated meta-analysis to investigate the association between MTHFR gene polymorphisms and susceptibility to lung cancer in Chinese people. METHODS: PubMed, EMBASE, WANFANG database, and CNKI were searched to collect eligible articles. The associations of MTHFR gene polymorphism with lung cancer risk were evaluated by calculating the pooled odds ratios (ORs) and the 95% confidence interval (CI). The dominant, recessive, heterozygous, homozygous, and allelic genetic models were used to calculate the combined ORs. RESULTS: A total of 16 eligible studies were identified in the present meta-analysis. Evidence from the pooled results indicated a significant association between the MTHFR C677T polymorphism and lung cancer susceptibility in Chinese people under the dominant, recessive, homozygous and allelic genetic models (T vs C: OR = 1.252, 95% CI, 1.090-1.437; TT vs CC: OR = 1.741, 95% CI, 1.252-2.420. (TT + CT) vs CC: OR = 1.227, 95% CI, 1.030-1.426. TT vs (CT + CC): OR = 1.606, 95% CI, 1.207-2.137). CONCLUSION: The present updated meta-analysis demonstrated that the MTHFR C677T polymorphism was significantly associated with susceptibility to lung cancer in Chinese people. Additional case-control studies with large sample sizes are needed to validate our findings.


Assuntos
Predisposição Genética para Doença , Neoplasias Pulmonares/genética , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Polimorfismo de Nucleotídeo Único , Grupo com Ancestrais do Continente Asiático/genética , China , Humanos , Neoplasias Pulmonares/epidemiologia
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