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1.
Inflammation ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31489527

RESUMO

Gut microbiota-derived metabolite trimethylamine N-oxide (TMAO) has recently been shown to promote inflammation in peripheral tissues and the central nervous system (CNS), contributing to the pathogenesis of various human diseases. Here, we examined whether the presence of high levels of circulating TMAO would influence central and peripheral inflammation and inflammatory hyperalgesia in a carrageenan (CG)-induced rat model of inflammation. Rats were treated with vehicle or TMAO in drinking water. After 2 weeks of treatment, rats received intraplantar injection of saline or CG into the hind paw. Acute nociception was unaltered in TMAO-treated rats that had elevated plasma TMAO. Following CG injection, TMAO-treated rats were significantly more sensitive to thermal and mechanical stimulation of the inflamed paw and displayed greater paw edema. Molecular studies revealed that CG injection induced increases in recruitment of neutrophils/macrophages in the paw and activation of microglia in the spinal cord, along with increased activation of nuclear factor (NF)-kB and production of proinflammatory mediators in both vehicle-treated rats and TMAO-treated rats. However, the increases in the above parameters were more pronounced in TMAO-treated rats. Moreover, TMAO treatment decreased protein levels of anti-inflammatory mediator regulator of G protein signaling (RGS)-10 in both saline-injected rats and CG-injected rats. These findings suggest that the presence of high levels of circulating TMAO downregulates anti-inflammatory mediator RGS10 in both peripheral tissues and the CNS, which may increase the susceptibility to inflammatory challenge-induced NF-kB activity, leading to greater increase in production of inflammatory mediators and consequent exacerbation of peripheral inflammation and inflammatory hyperalgesia.

2.
Small ; : e1903496, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31489786

RESUMO

Recently, Bi-doped Cs2 Ag0.6 Na0.4 InCl6 lead-free double perovskites demonstrating efficient warm-white emission have been reported. To enable the solution processing and enrich the application fields of this promising material, here a colloidal synthesis of Cs2 Ag1- x Nax In1- y Biy Cl6 nanocrystals is further developed. Different from its bulk states, the emission color temperatures of the nanocrystal can be tuned from 9759.7 to 4429.2 K by Na+ and Bi3+ incorporation. Furthermore, the newly developed nanocrystals can break the wavefunction symmetry of the self-trapped excitons by partial replacement of Ag+ ions with Na+ ions and consequently allow radiative recombination. Assisted with Bi3+ ions doping and ligand passivation, the photoluminescence quantum yield of the Cs2 Ag0.17 Na0.83 In0.88 Bi0.12 Cl6 nanocrystals is further promoted to 64%, which is the highest value for lead-free perovskite nanocrystals at present. The new colloidal nanocrystals with tunable color temperature and efficient photoluminescence are expected to greatly advance the research progress of lead-free perovskites in single-emitter-based white emitting materials and devices.

3.
Anim Biotechnol ; : 1-8, 2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31516062

RESUMO

Resistin (RETN) is a hormone secreted by adipocytes, which plays an important role in glucose and lipid metabolism. The aim of this study is to clone and obtain the full length open reading frame (ORF) of goat RETN gene sequence, and to reveal its molecular and expression characteristics. Simultaneously, we explore its effect on the differentiation of intramuscular preadipocytes in goat. The full length ORF sequence of goat RETN gene was cloned by RT-PCR technique, and bioinformatics analysis was performed though relevant biological softwares. In this study, the expression of RETN mRNA in goat tissues and intramuscular preadipocytes during differentiation was detected by qPCR technique. Furthermore, RNA interference was used to explore the effects of RETN on intramuscular preadipocytes differentiation in goat. The results showed that the cloned goat RETN gene sequence was 428 bp in length, of which the ORF was 330 bp, encoding 109 amino acids. Sequence analysis revealed that it had 12 phosphorylation sites and an O-glycosylation site, and its protein contained a signal peptide sequence. Also, the RETN gene is expressed in goat various analyzed tissues, and the results showed that the expression of RETN gene in lung tissue was higher than that in other analyzed tissues of goat (p < .01). Moreover, the expression level of RETN gene in the goat's intramuscular preadipocytes decreased first and then increased, and reached the highest on the fifth day, which was significantly higher than that of undifferentiated intramuscular preadipocytes (p < .001). After transfecting intramuscular preadipocyte with siRNA, we found that the mRNA level of RETN was significantly down-regulated by 70% and 87% (p < .01). Oil red O staining results showed that the interference of RETN gene can promote the differentiation of intramuscular preadipocytes. After knockdown of RETN with siRNA, the PPARγ, AP2, C/EBPα, C/EBPß and SREBP1 genes were significantly up-regulated (p < .01). Thus, it can be inferred that RETN inhibits the differentiation of goat intramuscular preadipocytes, probably through regulating the expression of C/EBPα, C/EBPß, PPARγ, AP2 and SREBP-1 genes.

4.
Hum Brain Mapp ; 2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31517428

RESUMO

An increasing number of studies in patients with generalized tonic-clonic seizures (GTCS) have reported the alteration of functional connectivity (FC) in many brain networks. However, little is known about the underlying temporal variability of FC in large-scale brain functional networks in patients. Recently, dynamic FC could provide novel insight into the physiological mechanisms in the brain. Here, we recruited 63 GTCS and 65 age- and sex-matched healthy controls. Dynamic FC approaches were used to evaluate alterations in the temporal variability of FC in patients at the region- and network-levels. In addition, two kinds of brain templates (>102 and > 103 regions) and two kinds of temporal variability FC approaches were adopted to verify the stability of the results. Patients showed increased FC variability in regions of the default mode network (DMN), ventral attention network (VAN) and motor-related areas. The DAN, VAN, and DMN illustrated enhanced FC variability at the within-network level. In addition, increased FC variabilities between networks were found between the DMN and cognition-related networks, including the VAN, dorsal attention network and frontal-parietal network in GTCS. Meanwhile, the alterations in FC variability were relatively consistent across different methods and templates. Therefore, the consistent alteration of FC variability would reflect a dynamic restructuring of the large-scale brain networks in patients with GTCS. Overly frequent information communication among cognition-related networks, especially in the DMN, might play a role in the epileptic activity and/or cognitive dysfunction in patients.

5.
Math Biosci Eng ; 16(5): 3936-3946, 2019 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-31499643

RESUMO

In this paper, the SEIR model is generalized by introducing an asymptomatic class to quantify the influence of wearing N95 facemasks in reducing the spread of influenza H1N1. What's more, we explain the control reproduction number Rc according to the biological meaning reasonably. Without any intervention, the basic reproduction number R0=1.83 and there will be a large outbreak of infectious diseases. If N95 facemasks are 50% effective in reducing susceptibility and infectivity, 50% of population wear them on the first day, the basic reproduction number will be decreased from 1.83 to 1.17 and the final size reduced from 73% to 2%.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31502887

RESUMO

Background: Osteoarthritis (OA) is the most prevailing osteoarticular disease, which typically involves chronic cartilage degeneration and synovitis. The latest research shows that circular RNAs (circRNAs) play a role in the development of a variety of diseases, including osteoarthrosis. Purposes: The aim of this study was to explore the expression of circRNAs in OA chondrocytes and predict biomarkers for diagnosis. Materials and Methods: The circRNA expression profile was analyzed through use of the Gene Spring software V13.0; differentially expressed circRNAs were screened over a process of OA. We validated the microarray data by quantitative real-time polymerase chain reaction analyses of OA chondrocytes and chondrocytes from normal controls. TargetScan software and miRanda software were used to predict network analysis of circRNA-miRNA interactions in cartilages. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analyses were applied to predict the functions of differentially expressed circRNAs. Results: Overall 1380 circRNAs were differentially expressed in OA chondrocytes in contrast to the normal articular cartilages (fold-change ≥2, p ≤ 0.05), consisting of 215 upregulated and 1165 downregulated circRNAs. After analyzing the differentially expressed circRNA genes, the top 20 enriched GO entries and the KEGG pathways were annotated. Furthermore, hsa_circrna_0032131 was identified for further analysis. The circRNA-miRNA network was constructed to represent the 10 most likely target genes associated with the validated circRNA. Conclusions: Our research suggests that some of the differentially expressed circRNAs in OA chondrocytes compared to normal chondrocytes are etiologically associated with the pathological process of OA. It was found that hsa_circRNA_0032131 likely participates in the initiation and progression of OA and has potential as a diagnostic marker. Clinical Relevance: To analyze the difference of circRNA expression profiles between OA and normal controls and explore biomarkers for diagnosis.

7.
Radiat Oncol ; 14(1): 161, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31481064

RESUMO

BACKGROUND: The prognosis of N categories for patients with non-surgical esophageal carcinoma based on the number of metastatic lymph nodes is controversial. The present study analyzes prognostic implications of the number, extent, and size of metastatic lymph nodes for patients with esophageal squamous cell carcinoma (ESCC) treated with definitive (chemo-)radiotherapy to provide more information on treatment strategy. METHODS: We reviewed 357 ESCC patients treated with definitive radiotherapy between January 2013 and March 2016 retrospectively. We assessed potential associations between the involved extent (N0, 1 region, 2 regions, and 3 regions), number (N0, 1-2, 3-6, and ≥ 7), and size (N0, ≤2 cm, and > 2 cm) of metastatic lymph nodes and overall survival. Multivariate analyses of the clinicopathological factors were performed using the Cox proportional hazard model. RESULTS: 5-year survival rates were 43.6% for patients in the N0 group and 29.3% in the N+ group (p = 0.001). Kaplan-Meier analyses for all cases revealed that there were significant differences in survival based on the extent (the OS rates at 3 years were 53.3% for patients in the N0 group, 45.7% in the 1 region-involved group, 28.0% in the 2 regions-involved group, and 13.3% in the 3 regions-involved group, P < 0.001), number (the OS rates at 3 years were 49.0% for patients in the 1-2 LNs group, 27.8% in the 3-6 LNs group, 0 in the ≥7LNs group, P < 0.001), and size (the OS rates at 3 years were 41.6% for patients in the LNs ≤2 cm group and 20.7% in the LNs > 2 cm group, P = 0.001) of metastatic LNs. One hundred seventy-two patients (48.2%) had experienced GTV failure, 157 (43.1%) had distant failure, 49 (13.7%) had out-of-GTV nodal failure, and 70 patients (19.6%) had no evidence of disease at the last follow-up. Nodal status correlated statistically with GTV failure. Patients with LN metastases in the abdominal region had worse survival rates than those with metastases in the other regions. The extent and number of metastatic LNs, T category, Primary tumor location, and chemotherapy were independent prognostic factors of OS in multivariate analyses. CONCLUSIONS: For patients with ESCC who received definitive (chemo-)radiotherapy, the number, extent, and size of metastatic LNs were prognostic factors, particularly of the T2/3 disease. Patients with LN metastases in the abdominal region had worse survival.

8.
J Cell Mol Med ; 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31430030

RESUMO

Depression is the most frequent psychiatric disorder in the world. Recent evidence has shown that stress-induced GABAergic dysfunction in the nucleus accumbens (NAc) contributed to the pathophysiology of depression. However, the molecular mechanisms underlying these pathological changes remain unclear. In this study, mice were constantly treated with the chronic unpredictable mild stress (CUMS) till showing depression-like behaviours expression. GABA synthesis, release and uptake in the NAc tissue were assessed by analysing the expression level of genes and proteins of Gad-1, VGAT and GAT-3 by qRT-PCR and Western blotting. The miRNA/mRNA network regulating GABA was constructed based on the bioinformatics prediction software and further validated by dual-luciferase reporter assay in vitro and qRT-PCR in vivo, respectively. Our results showed that the expression level of GAT-3, Gad-1 and VGAT mRNA and protein significantly decreased in the NAc tissue from CUMS-induced depression-like mice than that of control mice. However, miRNA-144-3p, miRNA-879-5p, miR-15b-5p and miRNA-582-5p that directly down-regulated the expression of Gad-1, VGAT and GAT-3 were increased. In the mRNA/miRNA regulatory GABA network, Gad-1 and VGAT were directly regulated by binding seed sequence of miR-144-3p, and miR-15b-5p, miR-879-5p could be served negative post-regulators by binding to the different sites of VGAT 3'-UTR. Chronic stress causes the impaired GABA synthesis, release and uptake by up-regulating miRNAs and down-regulating mRNAs and proteins, which may reveal the molecular mechanisms for the decreased GABA concentrations in the NAc tissue of CUMS-induced depression.

9.
Chemosphere ; 235: 900-907, 2019 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-31299703

RESUMO

Effluent organic matter (EfOM), which is composed of background natural organic matter (NOM), soluble microbial degradation products, and trace amounts of organic pollutants, can play an important role in the photodegradation of emerging pollutants in the effluent. In this study, the impact of organic pollutants, using fenofibrate acid (FNFA) as a representative, on the photodegradation of emerging contaminants, using bezafibrate (BZF) as a representative, in effluents was investigated. It is found that BZF undergo fast degradation in the presence of FNFA although BZF is recalcitrant to degradation under simulated sunlight irradiation. The promotional effect of FNFA is due to the generation of singlet oxygen (1O2) and hydrated electrons (e-aq). Based on the structures of the identified intermediates, 1O2 initiated oxidation and e-aq initiated reduction reactions were the main photodegradation pathways of BZF in the effluents. The toxicity of the main photodegradation intermediates for BZF and FNFA was higher than that of the parent compounds, and the acute toxicity increased during simulated sunlight irradiation. The results demonstrated that trace amounts of organic compounds in EfOM can play an important role in sensitizing the photodegradation of some emerging pollutants in the effluent.

10.
Water Res ; 162: 180-189, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31272043

RESUMO

2-Methylisoborneol (2-MIB) is one of the most common taste and odor (T&O) compounds in waterbodies and causes complaints from drinking water consumers. This is a case study of two water treatment plants taking the raw water from Lake Tai, the third largest lake in China. The relationships between the 2-MIB concentration and algae density, light change and nutrients of the raw water for the most recent four years were comprehensively investigated. The yearly variation pattern of the 2-MIB concentration in the raw water is closely related to the algae cell density. Both values reached peaks in July or August with concentrations as high as ∼500 ng/L and ∼1000 × 104 count/L, respectively. For the diurnal 2-MIB variation, the concentrations increase from 5 to 6 am, reach a peak value at ∼12 p.m., and then gradually decrease and achieve the lowest value at night. These results further confirm that 2-MIB is highly related to algae cell activity (e.g., photosynthesis). In addition, 2-MIB exists both inside and outside algae cells (i.e., intracellular and extracellular or bound and dissolved 2-MIB, respectively), and the percentage of the extracellular/dissolved portion was as high as 60% during the study period. Conventional water treatment processes (usually referrings to coagulation sedimentation and sand filtration, CSF) in WTPs have extremely unstable 2-MIB removal efficiencies (from -20% to >95%), which is mainly related to the existing forms of 2-MIB. The intracellular/bound portion can be effectively removed by CSF, especially sand filtration, while advanced water treatment processes are required to remove the dissolved 2-MIB. An ozone-activated carbon process is recommended, and the 2-MIB removal rate can reach 100%. This research has great theoretical and engineering value for treating water containing T&O compounds.

11.
J Physiol Sci ; 69(5): 779-790, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31286450

RESUMO

Intermittent hyperbaric oxygen exposure (IE-HBO) can protect the body against oxygen toxicity, but the underlying mechanisms are not very clear. Peroxiredoxin 6 (Prdx6) is a special endogenous antioxidative protein. We explored if the protective effects of IE-HBO are related to Prdx6. Mice were exposed to 280 kPa O2 for 60 min, followed by 30-min exposure to 20% O2/N2 mixture with equal pressure, repeated for six cycles. The Prdx6 protein level and non-selenium glutathione peroxidase (NSGPx) activity in the brain and lungs were then measured and the injury degree of lung and the oxidation level of brain and lung were evaluated. On this basis, the relationship between Prdx6 and IE-HBO's protection was explored. Generally, both IE-HBO and continuous exposure to HBO (CE-HBO) could increase the protein and mRNA levels of Prdx6, and such increases were more significant 24 h after cessation of exposure; moreover, the Prdx6 level of IE-HBO was higher than that of CE-HBO in both brain and lung, also more significantly 24 h after cessation of exposure. In addition, IE-HBO exposure could more effectively potentiate the activity of NSGPx and increase GSH content in brain and lung tissues. At the same time, it could reduce oxidation products in these tissues. IE-HBO could also provide protection for the lungs against injuries resulting from prolonged HBO exposure. These data showed that IE-HBO can potentiate the production and the activity of Prdx6 and consequently mitigate oxidative damages in brain and lungs. The influences of IE-HBO on Prdx6 may form an important basis for its protection against oxygen toxicity.

12.
Biomed Res Int ; 2019: 1580982, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31341888

RESUMO

Cardiac hypertrophy mainly predicts heart failure and is highly linked with sudden loss of lives. MicroRNAs (miRNAs) play essential roles in the development of cardiac hypertrophy through binding to corresponding mRNA targets. In this study, in order to investigate the roles of two mature forms of miRNA-195, miR-195-3p, and miR-195-5p, in vitro and in vivo models of cardiac hypertrophy were established by applying angiotensin II (Ang II) to H9c2 cardiomyocytes and infusing chronic Ang II to mice, respectively. We found that miR-195-5p was evidently equally upregulated in the in vitro and in vivo studies of cardiac hypertrophy induced by Ang II. High expressed miR-195-5p could adequately promote hypertrophy, whereas the suppression of miR-195-5p prevented hypertrophy of H9c2 cardiomyocytes under Ang II treatment. Furthermore, the luciferase reporter system demonstrated that MFN2 and FBWX7 were target genes of miR-195-5p, which negatively regulated the expression of these two genes in H9c2 cells. By contrast, in both models, expression of miR-195-3p was only slightly changed without statistical significance. In addition, we observed a trend towards decreased expression of hypertrophic markers by overexpressing miR-195-3p in AngII-treated H9c2 cardiomyocytes in vitro. Taken together, our study indicates that miR-195-5p promotes cardiac hypertrophy via targeting MFN2 and FBXW7 and may provide promising therapeutic strategies for interfering cardiac hypertrophy.

13.
Nat Commun ; 10(1): 3378, 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31358750

RESUMO

Steroidal C19-hydroxylation is pivotal to the synthesis of naturally occurring bioactive C19-OH steroids and 19-norsteroidal pharmaceuticals. However, realizing this transformation is proved to be challenging through either chemical or biological synthesis. Herein, we report a highly efficient method to synthesize 19-OH-cortexolone in 80% efficiency at the multi-gram scale. The obtained C19-OH-cortexolone can be readily transformed to various synthetically useful intermediates including the industrially valuable 19-OH-androstenedione, which can serve as a basis for synthesis of C19-functionalized steroids as well as 19-nor steroidal drugs. Using this biocatalytic C19-hydroxylation method, the unified synthesis of six C19-hydroxylated pregnanes is achieved in just 4 to 9 steps. In addition, the structure of sclerosteroid B is revised on the basis of our synthesis.

14.
Bioresour Technol ; 290: 121751, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31301571

RESUMO

The removal of antibiotics from wastewater has attracted much attention. In this research, an intimately coupled autarkical electrosorption (ES)-microbial fuel cell (MFC) system was developed for real-time removal of the antibiotic oxytetracycline (OTC) from wastewater. The removal efficiency was founded to be up to 98.8% at an OTC concentration of 2 mg/L with 3 g/L sodium acetate (NaAC) as co-substrate and 3 MFCs as power supply. The removal efficiencies increased in the ES unit and decreased in the MFC unit with increasing treating time. The adsorption of OTC on activated carbon fibers (ACFs) in the ES unit proceeds via chemical adsorption resulting from electrostatic attraction and cation exchange. The OTC degradation pathways in the MFC unit were proposed by identifying the intermediates with HPLC-MS/MS. The ACFs in the ES unit were proven to be recyclable and the coupled ES-MFC system is applicable for the removal of antibiotics from wastewater.

16.
Curr Top Med Chem ; 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31284863

RESUMO

The cannabinoid receptor 1 (CBR1) is involved in a variety of physiological pathways and has long been considered a golden target for therapeutic manipulation. A large body of evidence in both animal and human studies suggests that CB1R antagonism is highly effective for the treatment of obesity, metabolic disorders and drug addiction. However, the first-in-class CB1R antagonist/inverse agonist, rimonabant, though demonstrating effectiveness for obesity treatment and smoking cessation, displays serious psychiatric side effects, including anxiety, depression and even suicidal ideation, resulting in its eventual withdrawal from the European market. Several strategies are currently being pursued to circumvent the mechanisms leading to these side effects by developing neutral antagonists, peripherally restricted ligands, and allosteric modulators. In this review, we describe the progress in the development of therapeutics targeting the cannabinoid receptor 1 in the last two decades.

17.
Mol Hum Reprod ; 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31323679

RESUMO

Fibrotic tissue may contribute to the origin of some endometriosis-related symptoms, such as chronic pelvic pain and infertility. Alterations in the H19/miR-216a-5p/ACTA2 pathway may mediate the regulation of eutopic endometrial stromal cell (euESC) invasion and migration and may represent a potential mechanism underlying fibrous tissue formation or fibrosis in women with endometriosis. In this study, we aimed to determine the expression of H19 and ACTA2 in endometrial tissues of women with endometriosis. Two groups of 23 infertile women with endometriosis and 23 matched infertile women without endometriosis were investigated. Primary cultured cells of endometrial tissues were analysed using RT-PCR and western blotting (WB) to determine expression of H19 and ACTA2. EdU, CCK8, and Transwell assays were used to study the functions of H19 and ACTA2. HEK 293 cells were used for luciferase assays to study miR-216a-5p binding sites with H19 and ACTA2. We found that H19 and ACTA2 levels were significantly higher in endometriosis euESCs than in control euESCs (P < 0.05) and were positively correlated in endometriosis euESCs. Luciferase assays indicated that H19 regulates ACTA2 expression via competition for inhibitory miR-216a-5p binding sites. Our results indicate that alterations in the estrogen/H19/miR-216a-5p/ACTA2 pathway regulated endometriosis euESC invasion and migration. Downregulation of H19 or ACTA2 inhibited endometriosis euESC invasion and migration, however estrogen promoted endometriosis euESC invasion and migration via H19. The main limitation of our study was that experiments were conducted in vitro and further in-vivo studies are required in the future. However, our study showed that primary cultured cells represented endometriosis cells more clearly than cell lines.

18.
J Stroke Cerebrovasc Dis ; 28(9): 2543-2554, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31296475

RESUMO

BACKGROUND: Today there exists few intervention researches on cerebro-vascular function in populations at high risk of stroke in China. METHODS: Patients more than 40 years old, with at least 1 of stroke risk factors were recruited from outpatient department in 3 hospitals. A quasi-experimental design was performed by assigning participants into 3 groups: comprehensive intervention group, health education group, and control group. Participants in the control group received no intervention but were informed of risk factors of stroke. For health education group, a health education class was performed. Except to the health education program, participants in the comprehensive intervention group received an additional health life and behavior guidance. RESULTS: After the intervention, the Cerebro-Vascular Function Scores (CVFS) had significant differences among 3 groups (F = 5.252, P < 0.05). There was a significant increase in CVFS compared to the control group (P = 0.003, 95%CI: 1.552-8.493). Significantly changes in obesity were observed in comprehensive intervention group before and after the intervention (χ2 = 9.0747, P = 0.0026). The results of logistic regression showed that comprehensive intervention group had a significant decrease in prevalence of obesity (OR = 0.482, 95% CI: 0.242-0.961) compared to the control group. CONCLUSION: Health education on stroke in a high-risk population combined with guidance on proper health life and behavior can be effective in preventing stroke.


Assuntos
Circulação Cerebrovascular , Comportamentos Relacionados com a Saúde , Educação de Pacientes como Assunto/métodos , Serviços Preventivos de Saúde/métodos , Comportamento de Redução do Risco , Acidente Vascular Cerebral/prevenção & controle , Adulto , China/epidemiologia , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Estilo de Vida Saudável , Humanos , Masculino , Pessoa de Meia-Idade , Obesidade/epidemiologia , Obesidade/prevenção & controle , Prevalência , Fatores de Proteção , Medição de Risco , Fatores de Risco , Acidente Vascular Cerebral/epidemiologia , Acidente Vascular Cerebral/fisiopatologia , Acidente Vascular Cerebral/psicologia , Fatores de Tempo , Resultado do Tratamento
19.
Gene ; 719: 144007, 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31357024

RESUMO

Congenital bilateral absence of vas deferens (CBAVD), a frequent cause of obstructive azoospermia and male infertility in Chinese, is mainly due to mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. This study aim to explore the promoter region of CFTR gene in CBAVD patients and study the mutations by functional analysis, and to discuss the significance of mutation testing in this area. We performed screening analysis on 65 CBAVD patients and 50 controls to detect mutations in the CFTR gene, and studied the functions of promoter mutations using reporter gene constructs, transient transfection techniques and subsequent assessment of transcriptional activity and expression levels. Mutations c.-195C>A and c.-34C>T in the promoter region of the CFTR gene were detected in 4 of our Chinese CBAVD patients, one of which was novel (c.-195C>A) and located in the conservative area, as well as the binding site of SP1 transcription factor through the prediction of bioinformatics analysis. By reverse transcription qPCR assay and luciferase assay, we validated it as a functional disease-causing variant that down-regulates the CFTR gene expression, and this effect was related to the amount of transcription factors. This study was the first to explore the promoter region of the CFTR gene in Chinese, and we believe that mutations in this region are associated with Chinese CBAVD patients. We also suggest a systematic strategy for genotyping Chinese CBAVD couples, which should help in developing reproductive counseling.

20.
Anal Chim Acta ; 1076: 1-17, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31203952

RESUMO

Boronate affinity materials, as widely used sorbents for the enrichment of cis-diol-containing molecules, have been rapidly developed and increasingly utilized for various applications in recent years. cis-Diol-containing molecules, including saccharides, nucleosides, catecholamines, glycans and glycoproteins/glycopeptides, are major targets in the frontiers of many research areas, such as environmental analysis, the food industry and bioanalysis. As the analysis of these molecules usually suffers from the low abundance of targets and the high abundance of interference, selective enrichment is a fundamental step of sample preparation before analysis. In this review, we survey recent achievements of boronate affinity materials and their applications in sample preparation. We mainly focus on the fundamental considerations of materials as well as important applications in the past 3 years. Particularly, the effects of the substrate structure on the performance of boronate affinity materials, such as binding capacity, affinity, selectivity and working pH, will be discussed. Furthermore, the applications in sample preparation will also be introduced, with a main emphasis on what merits can be provided by boronate affinity materials to overcome the challenges in sample preparation.

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