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1.
Zhongguo Zhong Yao Za Zhi ; 45(3): 491-496, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32237505

RESUMO

Natural indigo, as one of the oldest dyes, is also a pivotal dye utilized in cotton fabrics today. A diversity of plants rich in indigo compounds belong to traditional Chinese herbal medicines. Indigo compounds have a variety of biological and pharmacological activities, including anticonvulsant, antibacterial, antifungal, antiviral and anticancer activities. A substantial progress in indigo biosynthesis has been made lately. This paper summarizes the value of indigo from the aspects of cultural history, biosynthetic pathways and the medicinal activities of its related derivatives involved in the pathways. In addition, the latest research advancements in indigo biosynthetic pathways is demonstrated in this paper, which would lay the theoretical foundation for the exploration and utilization of natural indigo.

2.
Zhongguo Zhen Jiu ; 40(1): 109-11, 2020 Jan 12.
Artigo em Chinês | MEDLINE | ID: mdl-31930910

RESUMO

A mini-infrared moxibustion instrument was developed on the base of carbon fiber heating film. This new type moxibustion instrument integrated the moxibusiton technique of TCM with modern technology. It is composed of a power module, an infrared generator module, a temperature sensor, a display screen and a main control panel. The carbon fiber is adopted as the material for infrared generator, which produces infrared rays in the range of the life light wave (from 8 to 15 µm), characterized as precise control of temperature, small gradient and wide range of temperature adjustment. The users can adjust the temperature and time of moxibustion by themselves. The instrument is small in size, light in weight, easy to carry and charge as well as comfortable and safe in application. It can be fixed directly at the required region without the posture restriction and be used whenever needed. Using PowerLab multichannel physiological recorder, the temperature carve is detected at different setting temperatures. The results show that the temperature is increased rapidly and stable.


Assuntos
Moxibustão , Pontos de Acupuntura , Fibra de Carbono , Temperatura Ambiente
3.
Chemosphere ; 240: 124789, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31561157

RESUMO

Poly- and perfluorinated compounds (PFCs) may induce potential endocrine-disrupting hormonal effects. However, the molecular mechanism of the toxicology of PFCs remains unclear, and the insufficient information is available on the biological activities of PFCs at present. In this study, the cell-based reporter gene assays were used to determine the agonistic activity of PFCs on the human pregnane X receptor (hPXR). The heuristic method combined with best subset modeling (HM-BSM) based on Dragon descriptors and comparative similarity indices analysis (CoMSIA) were employed to build classical quantitative structure-activity relationship (QSAR) and three-dimensional QSAR models, respectively. The applicability domain (AD) of the classical QSAR model was assessed. Both the HM-BSM and CoMSIA approaches demonstrated good robustness, predictive ability, and mechanistic interpretability. The r2 and leave-one-out cross-validation squared correlated coefficient (q2LOO) values were 0.872 and 0.759 for the HM-BSM, and 0.976 and 0.751 for the CoMSIA model, respectively. The hPXR agonistic activity of the PFCs predicted by the built HM-BSM and CoMSIA agreed well with experimental activity, with root mean square error (RMSE) values of 0.0803 and 0.117, respectively, and external validation squared correlated coefficients (q2EXT) of 0.972 and 0.932, respectively. The hPXR agonistic activity of PFCs was related to their molecular polarizability, charge and atomic mass. Hydrogen bonding and hydrophobic interactions constituted the primary intermolecular forces between PFCs and the hPXR. The developed models were used to screen the PFCs with high hPXR agonistic activity.


Assuntos
Fluorcarbonetos/química , Receptor de Pregnano X/química , Simulação por Computador , Heurística , Humanos , Ligações de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Relação Quantitativa Estrutura-Atividade
4.
Curr Med Sci ; 39(6): 1019-1028, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31845236

RESUMO

Gestational hypertension (GH) is a common complication during pregnancy. GH is regarded as a potential public health challenge for pregnant women and infants. Limited evidence has linked ambient air pollution to an increased GH risk. However, most of the studies were conducted in developed countries, with inconsistent results obtained. The present study was performed to explore whether exposure to particulate matters with an aerodynamic diameter < 2.5 (PM2.5) and ozone (O3) was related to elevated odds of GH in a Chinese population. This population-based cohort study involved 38 115 pregnant women in Wuhan, China. All information was collected from the Wuhan Maternal and Child Health Management Information System, using standardized quality control. The daily air pollutant data for PM2.5 and O3 were obtained from the 20 monitoring stations of the Wuhan Environmental Monitoring Center during 2014. The nearest monitor approach was applied to individual exposure assessment of PM2.5 and O3 for each participant. After adjusting for major confounders and other air pollutants, a 10 µg/m3 increase in PM2.5 and O3 concentrations was found to correlate to a 1.14-fold [95% confidence interval (95% CI): 1.09, 1.20] and a 1.05-fold (95% CI: 1.02, 1.07) increase in GH risk, respectively. Additionally, stronger relationships between GH risk and PM2.5 and O3 exposure were observed in women who conceived in winter and summer, respectively. These findings suggest that air pollutants may contribute to the development of GH.

5.
Math Biosci Eng ; 16(5): 4007-4035, 2019 05 06.
Artigo em Inglês | MEDLINE | ID: mdl-31499647

RESUMO

Fire-loaded cementitious material such as concrete experiences a rapid and dramatic pore pressure buildup, resulting in potential explosive spalling-sudden loss of the heated section-which can jeopardize the structure. Pore pressure buildup processes in heated concrete are closely related to the relative permeabilities of concrete to gas and liquid denoted by krg and krl, respectively. While krg has been widely investigated experimentally, krl is conventionally determined by semi-analytical meth-ods such as Mualem's model, the reliability of which has been questioned by indirect experimentation but is not fully understood. In this work, we discuss the potential overestimation of krl by conventional model in consideration of the achievements of previous research. Then, by using different models, the influences of krl on the pore pressure pg are shown and compared through numerical simulations with a well established thermo-hydro-chemical (THC) multifield framework, revealing that the conventional model provides smaller values of pg than other models. Finally, through a comparison with water con-tent results obtained from nuclear magnetic resonance (NMR) tests in publications [1], we prove that some other models produce results that are more agreeable than those of the conventional model, which cannot reproduce the steep increase in the moisture content with depth observed experimentally.

6.
Chem Commun (Camb) ; 55(76): 11394-11397, 2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31482882

RESUMO

A nitrogen-thermal approach via the reaction between transition metal species and N dopants affords us the ability to optimize the tradeoff between the number of exposed transition metal/carbon (exemplified by cobalt in this work) boundaries and the most pronounced interfacial rectifying contact to achieve the highly efficient and selective hydrogenation and dehydrogenation of N-heterocycle compounds in a reversible manner.

7.
J Asian Nat Prod Res ; : 1-14, 2019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-31345059

RESUMO

Xanthohumol (XN) exerts a specific cytotoxicity in B16-F10 melanoma cells with cytoplasmic vacuoles formation. Further investigation showed XN inhibited cell proliferation in a time- and dose-dependent manner along with down-regulation of mitogen-activated protein kinase and up-regulation of the endoplasmic reticulum (ER) stress marker Bip, CHOP and protein ubiquitination, which was relieved by the ER-stress inhibitor 4-PBA. Whereas no early apoptosis characteristics was identified during XN induced cell death.

8.
Am J Chin Med ; 47(3): 595-612, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31122040

RESUMO

Although Astragalus polysaccharide (APS) has been shown to have various pharmacological effects, there have been no studies concerning the inhibitory effects of APS on the radiation-induced bystander effects (RIBE). The aim of this study was to investigate whether APS could suppress RIBE damage by inhibiting cell growth, micronucleus (MN) formation and 53BP1 foci number increased in bone marrow mesenchymal stem cells (BMSCs), named bystander cells, as well as to explore its mechanism. In this study, APS decreased proliferation and colony rate of bystander cells by inducing cell cycle arrest at G1 phase via extrinsic and intrinsic DNA damage. Regarding mechanism, APS inhibited mitogen-activated protein kinase (MAPK) signal pathway by down-regulating the expression of the key proteins, phosphorylated JNK (p-JNK), phosphorylated ERK (p-ERK) but not phosphorylated P38 (p-P38), and down-regulating their downstream function protein and molecule, cyclooxygenase-2 (COX-2) and reactive oxygen species (ROS). Moreover, in bystander cells, APS inhibits expression of transforming growth factor ß receptor II (TGF- ß R II), a cell membrane receptor, resulting in lower ROS production and secretion via TGF- ß R-JNK/ERK-COX-2/ROS not P38 signaling. They gave a hint that the decreased RIBE damage induced by APS treatment involved TGF- ß R-JNK/ERK-COX-2/ROS down-regulation.


Assuntos
Astrágalo (Planta)/química , Efeito Espectador/efeitos dos fármacos , Carbono , Proliferação de Células/efeitos dos fármacos , Fase G1/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos da radiação , Polissacarídeos/farmacologia , Células Cultivadas , Ciclo-Oxigenase 2 , Dano ao DNA , Humanos , Fosforilação , Espécies Reativas de Oxigênio/metabolismo , Fator de Crescimento Transformador beta/metabolismo
9.
Org Lett ; 21(9): 2998-3002, 2019 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-30939024

RESUMO

An asymmetric transfer hydrogenation via dynamic kinetic resolution of a broad range of rac- α-(purin-9-yl)cyclopentones was first developed. A series of cis-ß-(purin-9-yl)cyclopentanols were obtained with up to 97% yield, >20/1 dr, and >99% ee. This also provides an efficient synthetic route to a variety of chiral carbocyclic nucleosides.


Assuntos
Cetonas/química , Nucleosídeos/síntese química , Purinas/química , Catálise , Complexos de Coordenação/química , Hidrogenação , Cinética , Rutênio/química , Estereoisomerismo
10.
Gynecol Oncol ; 150(3): 509-514, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29960711

RESUMO

OBJECTIVE: Recent epidemiological studies have investigated the associations between the use of bisphosphonates and the development of endometrial cancer and ovarian cancer; these studies have shown controversial results. Hence, this meta-analysis was conducted to evaluate the changes in the risks of developing endometrial and ovarian cancers after using bisphosphonates based on current evidence. METHODS: A comprehensive search was performed in the MEDLINE, EMBASE, and Web of Science databases through January 2017. The summary relative risk (RR) estimates for the effects of the use of bisphosphonates on the risks of developing endometrial and ovarian cancers were calculated using a random-effects model. RESULTS: Seven studies were included with a total of 6471 endometrial cancer cases (7 studies with 213,920 participants) and 6783 ovarian cancer cases (4 studies with 105,507 participants). This meta-analysis suggested that any use of bisphosphonates was associated with a significant 27% reduction in the risk of endometrial cancer (RR = 0.73, 95% CI: 0.58-0.93, P = 0.012), but the reduction in the risk of ovarian cancer (RR = 0.81, 95% CI: 0.58-1.14, P = 0.227) was not significant. The protective effects of the use of bisphosphonates against endometrial cancer are mainly found in postmenopausal women (RR = 0.53, 95% CI: 0.34-0.93, P = 0.012) or in those who have taken bisphosphonates for longer than 1 year (RR = 0.57, 95% CI: 0.35-0.93, P = 0.024). CONCLUSION: This meta-analysis suggests that the use of bisphosphonates is associated with a reduction in the risk of endometrial cancer but not ovarian cancer.


Assuntos
Difosfonatos/uso terapêutico , Neoplasias do Endométrio/epidemiologia , Neoplasias Ovarianas/epidemiologia , Feminino , Humanos , Pós-Menopausa , Fatores de Proteção , Fatores de Tempo
11.
J Vet Res ; 62(1): 121-128, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29978137

RESUMO

Introduction: Xylazine, a type of α2-adrenoceptors, is a commonly used drug in veterinary medicine. Xylazine-induced changes in the content of amino acid neurotransmitters - glycine (Gly) and aspartic acid (Asp), in different brain regions and neurons were studied. Material and Methods: Wistar rats were administered 50 mg/kg or 70 mg/kg of xylazine by intraperitoneal injection. In addition, in vitro experiments were conducted, in which neurons were treated with 15 µg/mL, 25 µg/mL, 35µg/mL, and 45 µg/mL of xylazine. Test methods were based on the enzyme-linked immunosorbent assays (ELISA). Results: During anaesthesia, Asp levels in each brain area were significantly lower compared to the control group. Except for the cerebrum, levels of Gly in other brain areas were significantly increased during the anaesthesia period. In vitro, xylazine-related neuron secretion of Gly increased significantly compared to the control group at 60 min and 90 min. Moreover, xylazine caused a significant decrease in the levels of Asp secreted by neurons at 20 min, but gradually returned to the level of the control group. Conclusion: The data showed that during anaesthesia the overall levels of Asp decreased and overall levels of Gly increased. In addition, the inhibitory effect of xylazine on Asp and the promotion of Gly were dose-dependent. Our data showed that different effects of xylazine on excitatory and inhibitory neurotransmitters provided a theoretical basis for the mechanism of xylazine activity in clinical anaesthesia.

12.
Medicine (Baltimore) ; 97(6): e9548, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29419662

RESUMO

OBJECTIVE: The controversy remains as to whether immediate prosthetic breast reconstruction with postmastectomy radiation therapy (PMRT) is associated with acceptable complications and aesthetic outcomes. The aim of this meta-analysis was to provide a pooled analysis of comparative clinical studies that evaluated breast cancer patients who were treated with a mastectomy and an immediate prosthetic breast reconstruction to compare the complications and satisfaction of those who underwent or did not undergo PMRT. METHODS: According to the recommendations of the Cochrane Collaboration, we established a rigorous study protocol. We performed a systematic electronic search of the PubMed and Embase databases to identify articles for inclusion in our meta-analysis. Reconstruction failure, overall complications, capsular contracture, and patient satisfaction were analyzed individually. RESULTS: Fifteen controlled trials were included, comprising 5314 patients (1069 PMRT vs 4245 non-PMRT). Primary outcomes revealed a statistically significant increase in overall complications [odds ratio (OR) 3.45; 95% confidence interval (95% CI) 2.62-4.54; P < .00001], reconstruction failure (OR: 2.59; 95% CI 1.46-4.62; P = .001), and capsular contracture (OR: 5.26, 95% CI: 2.73-10.13, P < .00001) after receiving PMRT. CONCLUSION: Our review found that PMRT for patients who underwent immediate implant-based breast reconstruction led to higher risks of reconstruction failure, overall complications, and capsular contracture. However, it is still the standard adjuvant therapy for mastectomy patients who have opted for immediate implant-based breast reconstruction.


Assuntos
Implante Mamário/métodos , Neoplasias da Mama/radioterapia , Radioterapia Adjuvante/métodos , Neoplasias da Mama/cirurgia , Feminino , Humanos , Mastectomia/métodos , Resultado do Tratamento
13.
J Huazhong Univ Sci Technolog Med Sci ; 37(4): 605-611, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28786063

RESUMO

It is recognized that prenatal care plays an important role in reducing adverse birth. Chinese pregnant women with medical condition were required to seek additional health care based on the recommended at least 5 times health care visits. This study was to estimate the association between prenatal care utilization (PCU) and preterm birth (PTB), and to investigate if medical conditions during pregnancy modified the association. This population-based case control study sampled women with PTB as cases; one control for each case was randomly selected from women with term births. The Electronic Perinatal Health Care Information System (EPHCIS) and a questionnaire were used for data collection. The PCU was measured by a renewed Prenatal Care Utilization (APNCU) index. Logistic regression models were used to estimate odds ratios (OR) and the 95% confidence interval (95% CI). Totally, 2393 women with PTBs and 4263 women with term births were collected. In this study, 695 (10.5%) women experienced inadequate prenatal care, and 5131 (77.1%) received adequate plus prenatal care. Inadequate PCU was associated with PTB (adjusted OR: 1.41, 95% CI: 1.32-1.84); the similar positive association was found between adequate plus PCU and PTB. Among women with medical conditions, these associations still existed; but among women without medical conditions, the association between inadequate PCU and PTB disappeared. Our data suggests that women receiving inappropriate PCU are at an increased risk of having PTB, but it does depend on whether the woman has a medical condition during pregnancy.


Assuntos
Grupo com Ancestrais do Continente Asiático , Nascimento Prematuro/epidemiologia , Cuidado Pré-Natal/estatística & dados numéricos , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Fatores de Risco
14.
Toxicology ; 380: 23-29, 2017 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-28115241

RESUMO

Poly- and perfluorinated compounds (PFCs), which have been detected worldwide in human blood, surface water and house dust, are suspected to induce potential endocrine-disrupting hormonal effects. In this study, cell-based reporter gene assays were used to determine the activity of a variety of PFCs against the human pregnane X receptor (hPXR) to identify the critical structural feature of PFCs related to their hPXR activity. Molecular docking studies combined with site-directed mutagenesis were employed to investigate the mechanism by which PFCs interact with and activate hPXR. We found that all tested PFCs can activate hPXR. The hPXR activity of the PFCs correlates with the carbon chain length and the functional group of the chemicals. Hydrogen bonding was characteristic of the interaction between PFCs and hPXR. We also identified the key residues within the hPXR ligand-binding pocket responsible for PFC-hPXR interaction. The outcome of the present study threw a light on the mechanism by which PFCs activate hPXR. PFCs may pose some potential endocrine-disrupting hormonal effects via activation of hPXR.


Assuntos
Ácidos Alcanossulfônicos/toxicidade , Caprilatos/toxicidade , Disruptores Endócrinos/toxicidade , Fluorcarbonetos/toxicidade , Receptores de Esteroides/metabolismo , Genes Reporter , Células Hep G2 , Humanos , Interações Hidrofóbicas e Hidrofílicas , Simulação de Acoplamento Molecular , Receptor de Pregnano X , Receptores de Esteroides/genética
15.
Huan Jing Ke Xue ; 38(5): 2030-2035, 2017 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965110

RESUMO

Quinoline is one of the common refractory organic pollutants in coking wastewater. An aerobic bacterial strain KDQ3 capable of utilizing quinoline as the sole source of carbon, nitrogen and energy was isolated from activated sludge of a coking wastewater treatment plant. The morphological properties and the 16S rDNA sequence identified KDQ3 as Ochrobactrum sp.. The optimized temperature and initial pH for quinoline degradation were 37℃ and 7.0-8.0, and the degradation kinetics fit with Haldane's model. KDQ3 could degrade 200 mg·L-1 quinoline in the presence of 10.4 mg·L-1 hexavalent chromium. In addition, KDQ3 was able to degrade quinoline in real coking wastewater of aerobic tank and improve the removal of COD, indicating that KDQ3 had the potential of bioaugmentation for removal of quinoline from coking wastewater.


Assuntos
Coque , Ochrobactrum/metabolismo , Quinolinas/isolamento & purificação , Esgotos/microbiologia , Águas Residuárias , Biodegradação Ambiental
16.
J Huazhong Univ Sci Technolog Med Sci ; 36(3): 463-468, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27376821

RESUMO

The human pregnane X receptor (hPXR) plays a critical role in the metabolism, transport and clearance of xenobiotics in the liver and intestine. The hPXR can be activated by a structurally diverse of drugs to initiate clinically relevant drug-drug interactions. In this article, in silico investigation was performed on a structurally diverse set of drugs to identify critical structural features greatly related to their agonist activity towards hPXR. Heuristic method (HM)-Best Subset Modeling (BSM) and HM-Polynomial Neural Networks (PNN) were utilized to develop the linear and non-linear quantitative structure-activity relationship models. The applicability domain (AD) of the models was assessed by Williams plot. Statistically reliable models with good predictive power and explain were achieved (for HM-BSM, r (2)=0.881, q LOO (2) =0.797, q EXT (2) =0.674; for HM-PNN, r (2)=0.882, q LOO (2) =0.856, q EXT (2) =0.655). The developed models indicated that molecular aromatic and electric property, molecular weight and complexity may govern agonist activity of a structurally diverse set of drugs to hPXR.


Assuntos
Modelos Estatísticos , Relação Quantitativa Estrutura-Atividade , Receptores de Esteroides/agonistas , Bibliotecas de Moléculas Pequenas/química , Simulação por Computador , Humanos , Peso Molecular , Receptor de Pregnano X , Receptores de Esteroides/química , Eletricidade Estática
17.
Nan Fang Yi Ke Da Xue Xue Bao ; 36(6): 857-62, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-27320892

RESUMO

OBJECTIVE: To investigate the antitumor effect of lycorine on renal cell carcinoma ACHN cells and explore the possible mechanism. METHODS: We used flow cytometry to examine the effect of lycorine on ACHN cell cycle and apoptosis. The cell proliferation, migration and invasion were assessed with MTS assay, wound healing assay, and Transwell assay, respectively. Colony forming assay was performed, and the mRNA and protein levels of Bax, Bcl-2, survivin, caspase-3, cyclin D1 and CDK4 were measured with qRT-PCR and Western blotting. RESULTS: Lycorine obviously inhibited the proliferation of ACHN cells with an IC(50) of 24.34 µmol/L. Lycorine also induced apoptosis of ACHN cells, caused cell cycle arrest at G(0)/G(1) phase, and suppressed the colony forming ability of the cells in a dose-dependent manner. The migration and invasion of ACHN cells were significantly inhibited by 5 µmol/L lycorine. Lycorine up-regulated the mRNA levels of CDK4, Bax, caspase-3 while down-regulated the levels of survivin, Bcl-2 and Cyclin D1; the protein levels of CDK4 and Bax were increased and cyclin D1, Bcl-2 and surviving expressions were decreased, but caspase-3 expression showed no significant changes following the treatment. CONCLUSION: Lycorine has obvious antitumor effect against ACHN cells, suggesting its value as a new therapeutic agent for renal cell carcinoma.


Assuntos
Alcaloides de Amaryllidaceae/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Apoptose , Carcinoma de Células Renais/patologia , Fenantridinas/farmacologia , Caspase 3/metabolismo , Pontos de Checagem do Ciclo Celular , Linhagem Celular Tumoral/efeitos dos fármacos , Proliferação de Células , Ciclina D1/metabolismo , Quinase 4 Dependente de Ciclina/metabolismo , Humanos , Proteínas Inibidoras de Apoptose/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Survivina , Proteína X Associada a bcl-2/metabolismo
18.
Aesthet Surg J ; 36(6): 712-7, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27178901

RESUMO

BACKGROUND: Despite its increasing usage of facial applications, there is a paucity of objective data regarding calcium hydroxylapatite (CaHA). OBJECTIVES: To systematically evaluate the complications from CaHA injection for facial soft tissue augmentation. METHODS: Published studies on CaHA injection for facial soft tissue enhancement were identified through searches of the PubMed, EMBASE, and Cochrane Controlled Trial databases. Only randomized, controlled trials comparing CaHA injection to either placebo or an active comparator for facial cosmetic use were included. The outcome measures were the count (n) and frequency (%) of each complication, including edema (swelling), erythema (redness), ecchymosis (bruising), pain, pruritus (itching), hematomas, nodules, and extrusions. RESULTS: Four studies on nasolabial fold (NLF) injection of CaHA consisting of two subgroups were included: (i) a CaHA-lidocaine vs CaHA subgroup and (ii) a CaHA vs hyaluronic acid (HA) subgroup. The addition of lidocaine to CaHA therapy displayed no significant effect on edema (RR (95% CI): 1.07 (0.94-1.21), P = .311), erythema (RR (95% CI): 0.91 (0.66-1.24), P = .544), ecchymosis (RR (95% CI): 1.04 (0.71-1.52), P = .843), pain (RR (95% CI): 0.88 (0.58-1.33), P = .553), or pruritus (RR (95% CI): 0.82 (0.45-1.50), P = .515). There was no significant difference between CaHA vs HA for hematomas (RR (95% CI): 0.24 (0.01-4.31), P = .332) or nodules (RR (95% CI): 0.18 (0.01-6.62), P = .353). There was no significant publication bias detected in either subgroup (Begg's test P > 0.05). CONCLUSIONS: These findings support the addition of lidocaine to NLF injection of CaHA and suggest an equivalence between CaHA and HA with respect to hematoma and nodule formation. LEVEL OF EVIDENCE 2: Risk.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Técnicas Cosméticas/efeitos adversos , Durapatita/administração & dosagem , Anestésicos Locais/administração & dosagem , Materiais Biocompatíveis/efeitos adversos , Durapatita/efeitos adversos , Humanos , Injeções , Lidocaína/administração & dosagem , Sulco Nasogeniano , Dor/etiologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Envelhecimento da Pele
19.
Plast Reconstr Surg ; 137(4): 690e-699e, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27018697

RESUMO

BACKGROUND: Silicone rubber and silicone rubber-based materials have been used as medical tissue implants in the field of plastic surgery for many years, but there are still some reports of adverse reactions to long-term implants. Earlier studies have shown that ion implantation could enhance the biocompatibility of biomaterials. However, whether ion implantation has a good effect on silicone rubber is unknown. METHODS: Three types of carbon ion silicone rubber were obtained by implanting three doses of carbon ions. Then, the antibacterial adhesion properties and the in vivo host responses were evaluated. The antibacterial adhesion properties were examined by plate colony counting, fluorescence staining, and scanning electron microscopic observation. The host responses were evaluated by surveying inflammation and fiber capsule formation that developed after subcutaneous implantation in Sprague-Dawley rats for 7, 30, 90, and 180 days. In addition, the possible mechanism by which ion implantation enhanced the biocompatibility of the biomaterial was investigated and discussed. RESULTS: Carbon ion silicone rubber exhibits less bacterial adhesion, less collagen deposition, and thinner and weaker tissue capsules. Immunohistochemical staining results for CD4, tumor necrosis factor-α, α-smooth muscle actin, and elastin showed the possible mechanism enhancing the biocompatibility of silicone rubber. These data indicate that carbon ion silicone rubber exhibits good antibacterial adhesion properties and triggers thinner and weaker tissue capsules. In addition, high surface roughness and high zeta potential may be the main factors that induce the unique biocompatibility of carbon ion silicone rubber. CONCLUSION: Ion implantation should be considered for further investigation and application, and carbon ion silicone rubber could be a better biomaterial to decrease silicone rubber-initiated complications.


Assuntos
Materiais Biocompatíveis , Carbono , Próteses e Implantes , Elastômeros de Silicone , Animais , Aderência Bacteriana , Materiais Biocompatíveis/química , Carbono/química , Feminino , Íons , Próteses e Implantes/microbiologia , Ratos , Ratos Sprague-Dawley , Elastômeros de Silicone/química
20.
J Dermatol ; 43(6): 674-81, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26602869

RESUMO

After skin trauma, regional epidermal cell migration mediates the re-epithelialization of the wound surface, which is an important step for wound healing, yet the underlying molecular regulatory mechanism is unclear. In the current study, HaCaT cells were maintained under different oxygen concentrations (1%, 21%, 40% and 65%). Technologies including immunofluorescence staining, wound scratch, transwell invasion, western blot and low-expression lentiviral vector were utilized to observe the changes in microtubule dynamics and the microtubule-associated protein (MAP)4 expression. MAP4's effect on cell migration under different oxygen concentrations was also studied. The results showed that under hyperoxic (40% and 65%) and hypoxic (1%) conditions, HaCaT cells were able to regulate cell microtubule dynamics by MAP4, thus promoting cell migration. On the other hand, MAP4 silencing through targeted shRNA attenuated HaCaT cell migration under the above oxygen concentrations. These results imply that MAP4 plays an important role in epidermal cell migration under different oxygen concentrations.


Assuntos
Movimento Celular , Proteínas Associadas aos Microtúbulos/fisiologia , Oxigênio/fisiologia , Cicatrização , Linhagem Celular , Células Epidérmicas , Técnicas de Silenciamento de Genes , Humanos
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