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1.
Curr Genet ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040734

RESUMO

Serine/arginine (SR) proteins play significant roles in pre-mRNA splicing in eukaryotes. To investigate how gene expression influences fungal development and pathogenicity in Fusarium graminearum, a causal agent of Fusarium head blight (FHB) of wheat and barley, our previous study identified a SR protein FgSrp1 in F. graminearum, and showed that it is important for conidiation, plant infection and pre-mRNA processing. In this study, we identified another SR protein FgSrp2 in F. graminearum, which is orthologous to Schizosaccharomyces pombe Srp2. Our data showed that, whereas yeast Srp2 is essential for growth, deletion of FgSRP2 resulted in only slight defects in vegetative growth and perithecia melanization. FgSrp2 localized to the nucleus and both its N- and C-terminal regions were important for the localization to the nucleus. FgSrp2 interacted with FgSrp1 to form a complex in vivo. Double deletion of FgSRP1 and FgSRP2 revealed that they had overlapping functions in vegetative growth and sexual reproduction. RNA-seq analysis revealed that, although deletion of FgSRP2 alone had minimal effects, deletion of both FgSRP1 and FgSRP2 caused significant changes in gene transcription and RNA splicing. Overall, our results indicated that FgSrp2 regulates vegetative growth, sexual reproduction and pre-mRNA processing by interacting with FgSrp1.

2.
Mol Plant Pathol ; 21(3): 330-348, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31916390

RESUMO

The basal ascomycetes in genus Taphrina have strict host specificity and coevolution with their host plants, making them appealing models for studying the genomic basis of ecological divergence and host adaption. We therefore performed genome sequencing and comparative genomics of different Taphrina species with distinct host ranges to reveal their evolution. We identified frequent chromosomal rearrangements and highly dynamic lineage-specific (LS) genomic regions in Taphrina genomes. The LS regions occur at the flanking regions of chromosomal breakpoints, and are greatly enriched for DNA repeats, non-core genes, and in planta up-regulated genes. Furthermore, we identified hundreds of candidate secreted effector proteins (CSEPs) that are commonly organized in gene clusters that form distinct AT-rich isochore-like regions. Nearly half of the CSEPs constitute two novel superfamilies with modular structures unique to Taphrina. These CSEPs are commonly up-regulated during infection, enriched in the LS regions, evolved faster, and underwent extensive gene gain and loss in different species. In addition to displaying signatures of positive selection, functional characterization of selected CSEP genes confirmed their roles in suppression of plant defence responses. Overall, our results showed that extensive chromosomal rearrangements and rapidly evolving CSEP superfamilies play important roles in speciation and host adaptation in the early-branching ascomycetous fungi.

3.
Medicine (Baltimore) ; 98(44): e17793, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689855

RESUMO

RATIONALE: According to the literature reports and clinical studies on alopecia areata (AA) from 2008 to 2018, most clinical treatments have been oral drugs and external ointments. At present, systemic immunosuppressive therapy has been widely used in AA, but there are various side effects such as elevated liver enzymes, gastrointestinal discomfort, poor drug compliance, and repeated illness. We present a case report describing a traditional medicine treatment for AA that uses an ethnic therapy of Zhuang medicine, a kind of Traditional Chinese Medicine, namely, medicated thread moxibustion. PATIENT CONCERNS: A 36-year-old man endured AA after going through a family misfortune. Half a year ago, his father passed away suddenly. Since then, he suffered continuous anguish, alcoholism and hair loss, especially in the past 2 months. A coin-shaped area of hair loss began to appear at the top of his head and gradually expanded to the surrounding region. DIAGNOSES: A diagnosis of AA was made in the dermatology department of a local hospital. INTERVENTIONS: The patient was treated with the medicated thread moxibustion method of Traditional Zhuang Medicine at the Kuihua (special points of Zhuang medicine), Zusanli (ST 36), Xuehai (SP 10), Baihui (DU 20), and Taichong (LR 3) points every other day for 4 weeks. OUTCOMES: The area of hair loss showed slight improvement after 1 week of treatment. Only just a sprinkling of wooly hairs, whose color and thickness were similar to those of fine facial hairs, began to emerge sporadically from the follicles; they could be seen only in a bright light. When the patient saw the obvious curative effect, we continued the treatment for 2 weeks with the patient's consent. Three weeks later, the patchy AA area was covered with small cotton-like hairs of different lengths and uneven colors. LESSONS: The medicated thread moxibustion method of Zhuang medicine can be an effective alternative treatment in patients with AA.


Assuntos
Alopecia em Áreas/terapia , Medicina Tradicional Chinesa/métodos , Moxibustão/métodos , Adulto , Humanos , Masculino , Resultado do Tratamento
4.
Front Microbiol ; 10: 1419, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31293550

RESUMO

Controlling aflatoxigenic Aspergillus flavus and aflatoxins (AFs) in grains and food during storage is a great challenge to humans worldwide. Alcaligenes faecalis N1-4 isolated from tea rhizosphere soil can produce abundant antifungal volatiles, and greatly inhibited the growth of A. flavus in un-contacted face-to-face dual culture testing. Gas chromatography tandem mass spectrometry revealed that dimethyl disulfide (DMDS) and methyl isovalerate (MI) were two abundant compounds in the volatile profiles of N1-4. DMDS was found to have the highest relative abundance (69.90%, to the total peak area) in N1-4, which prevented the conidia germination and mycelial growth of A. flavus at 50 and 100 µL/L, respectively. The effective concentration for MI against A. flavus is 200 µL/L. Additionally, Real-time quantitative PCR analysis proved that the expression of 12 important genes in aflatoxin biosynthesis pathway was reduced by these volatiles, and eight genes were down regulated by 4.39 to 32.25-folds compared to control treatment with significant differences. And the A. flavus infection and AFs contamination in groundnut, maize, rice and soybean of high water activity were completely inhibited by volatiles from N1-4 in storage. Scanning electron microscope further proved that A. flavus conidia inoculated on peanuts surface were severely damaged by volatiles from N1-4. Furthermore, strain N1-4 showed broad and antifungal activity to other six important plant pathogens including Fusarium graminearum, F. equiseti, Alternaria alternata, Botrytis cinerea, Aspergillus niger, and Colletotrichum graminicola. Thus, A. faecalis N1-4 and volatile DMDS and MI may have potential to be used as biocontrol agents to control A. flavus and AFs during storage.

5.
Sci Total Environ ; 686: 788-796, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31195286

RESUMO

In China groundwater contamination has become a serious problem. The assessment and remediation of contaminated sites are greatly important. Moreover, only few studies deal with the influence of metal speciation on human health risk assessment. This paper proposed a comprehensive assessment methodology combined human health risk assessment and groundwater vulnerability assessment for contaminated area, exploring a more reasonable model for the Cd morphology simulation, and utilizing accurate method to calculate the average daily dose by the weight analysis. PHREEQC and Visual MINTEQ were applied and compared to the morphology simulation, the modified of average daily dose and non-modified of average daily dose was investigated, carcinogenic and non-carcinogenic risks were calculated subsequently. The groundwater vulnerability was assessed by the DRASTIC model, the DRASTIC index as the indicator of groundwater vulnerability. The morphology simulation results showed 20 and 13 metal species which were simulated by PHREEQC and Visual MINTEQ, respectively. Cd2+ and CdCl+ were the primary species of Cd in groundwater. The modified average daily dose showed less than the average daily dose in results. The carcinogenic risks showed the species of Cd2+ and CdCl+ were simulated by PHREEQC which were harmful to human health. The DRASTIC index was ranged from 109 to 134 in the studied area, which showed that the area was susceptible to pollution. This method provided a more effective risk assessment model and supplied a fundamental advice for government policy-making and site remediation.

6.
Chin J Integr Med ; 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-31111424

RESUMO

OBJECTIVE: To observe the imbalance of anatomical and functional innervation factors of sympathetic nerves, nerve growth factor (NGF) and leukemia inhibitory factor (LIF), in salt-sensitive hypertensive heart failure rats and to explore the effects of treatment with Guizhi Decoction () on sympathetic remodeling by inhibiting cholinergic transdifferentiation. METHODS: SS-13BN and Dahl salt-sensitive (DS) rats were divided into 3 groups: SS-13BN group (control group, n=9), DS group (model group, n=9) and GS group (Guizhi Decoction, n=9). After 10 weeks of a high-salt diet, the GS group rats were given Guizhi Decoction and other two groups were given saline at an equal volume as a vehicle. After 4 weeks' intragastric administration, rats were executed to detect the relevant indicators. Echocardiography and plasma n-terminal pro-B type natriuretic peptide (NT-proBNP) levels were used to assess cardiac function. Noradrenaline (NA) levels in the plasma and myocardium were detected to evaluate the sympathetic function. NGF and LIF expression were detected in the myocardium by Western blot or quantitative real-time PCR. Double immunofluorescence or Western blot was used to detect tyrosine hydroxylase (TH), choline acetyltransferase (CHAT) and growth associated protein 43 (GAP43) in order to reflect anatomical and functional changes of sympathetic nerves. RESULTS: DS group had anatomical and functional deterioration of sympathetic nerves in the decompensation period of heart failure compared with SS-13BN group. Compared with the DS group, Guizhi Decoction significantly decreased the expression of LIF mRNA/protein (P<0.01), increased the expression of NGF (P<0.05 or P<0.01), enhanced the levels of TH+/GAP43+ and TH+/CHAT+ positive nerve fibers (P<0.01), and improved the protein expression of TH and GAP43 in left ventricle, but had no effect on CHAT (P>0.05). Guizhi Decoction inhibited inflammatory infiltration and collagen deposition of myocardial injury, increased the content of myocardial NA (P<0.05), reduced the plasma NA level (P<0.01), improved cardiac function (P<0.01), and improved weight and blood pressure to some extent (P<0.05), compared with DS group. CONCLUSIONS: Guizhi Decoction could inhibit cholinergic transdifferentiation of sympathetic nerves, improve the anatomical and functional denervation of sympathetic nerves, and delay the progression of decompensated heart failure. The mechanism may be associated with the correction of the imbalance of NGF and LIF.

7.
Ecotoxicol Environ Saf ; 180: 95-105, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31078021

RESUMO

Assessing human health risk using spatiotemporal migration and geochemical evolution concurrently in an area where the groundwater is contaminated with heavy metals can provide more instructive information to protect specific potential negative impacts on human health. In this research, we established a model of long-term assessment of human health risk for metal contaminated groundwater by coupling two models: the geochemical (based on the law of chemical mass balance) model and the hydrodynamics module. The hydrodynamics module is used to initially identify the total temporal concentration of various elements, and the chemical mass balance module is used to gain the concentration and ionic activity of various toxic elements according to the range of environmental pH. Effective concentrations calculated using activity weight (based on speciation and ionic activity) were introduced into the formula for risk analysis. The results of the study show that, with the exploitation and recharge of groundwater, the non-carcinogenic and carcinogenic health risks cannot be reduced to acceptable levels until 18 and 22 years, respectively. The calculated risk values of using the coupling model are lower than that of statistics or single hydrokinetics. The sensitivity analysis results show that this model is reliable. The recharge, pH and the permeability coefficient are defined as the most sensitive factors.


Assuntos
Exposição Ambiental/análise , Água Subterrânea/química , Metais Pesados/análise , Modelos Teóricos , Poluentes Químicos da Água/análise , Monitoramento Ambiental , Humanos , Hidrodinâmica , Medição de Risco
8.
J Hazard Mater ; 369: 770-779, 2019 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-30851517

RESUMO

In this study, MnO2/MWCNT hybrids, prepared using a solvothermal method, were coated onto Ni foam and then used as a cathode for tetrabromobisphenol A (TBBPA) degradation. The reaction was confirmed to exhibit the pseudo first-order kinetics. Compared with the original Ni foam cathode, the fabricated electrode exhibited higher catalytic activity, attributed to its strong cross-linking and ability to produce catalytic free radicals. Radical scavenger experiments revealed that O2- and OH were involved in the decomposition of TBBPA. The effects of current density, pH, catalyst dosage, and initial TBBPA concentration on removal efficiency were further studied. An optimal removal rate of 98.3% was achieved while the rate constant reached values up to 0.07293 min-1 and the debromination rate was more than 75.4% within 60 min. The electrode showed high catalytic performance and low catalyst loss after 10 cycles, indicating its excellent stability and reusability. The probable mechanism and pathway of TBBPA degradation were suggested based on the analysis of intermediate products. It could be inferred that the decomposition of TBBPA involved CC bond breaks (oxidation) and debromination (reduction). The MnO2/MWCNT-Ni foam could be a promising cathode material for electrochemical degradation of halogenated organic compounds.

10.
Ecotoxicol Environ Saf ; 171: 211-221, 2019 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-30611039

RESUMO

Enriched and bio-refractory soil heavy metals (SHMs) originate from the underground mineral, which supplies energy and materials for the development of economy and industry. Investigating soil metal contents and their adverse health impacts is the principal concern associated metal contaminated industrial areas, including both current assessments and future projections. In this research, we create a novel spatiotemporal model of SHMs prediction and risk characterization for future by citing a rigorous theory of industrial economics, and time series of activity intensity changes of various pollution sources are forecasted. The dynamic change of source contributions is quantitatively resolved and the mean SHMs concentrations are estimated by classical formulas for heavy metal accumulation. Human health risk in the future is described in a manner of time series. The results of the case study show that contribution rates of the five sources of the six metals change continuously over time. Pb, Cd and As assume the highest growth rates (400%, 500% and 165%), while Zn, Ni, Cr possesses relatively lower growth (< 130%), compared to their corresponding background values. Health risk of local sensitive population (children) is estimated at exceeding threshold in 2022 (non-carcinogenic) and 2012 (carcinogenic), and the upward trend will continue. Traffic emission, agriculture and household garbage are identified as major risky sources in the coming decades at the studied area, and improvement measures are recommended. Although a degree of uncertainties exists, the overall tendency is a conservative bias for chemical risk. Additionally, this paper is the first to explore a methodology of predicting future SHMs and associated human health risk, based on industrial economics and temporal source apportionment.


Assuntos
Monitoramento Ambiental/métodos , Indústrias , Metais Pesados/análise , Modelos Teóricos , Poluentes do Solo/análise , Solo/química , Agricultura , Criança , China , Monitoramento Ambiental/economia , Previsões , Humanos , Indústrias/economia , Medição de Risco
11.
Environ Pollut ; 244: 93-101, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30321715

RESUMO

In this study, a multi-component catalyst, ß-cyclodextrin (ß-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal. The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated. Compared with Fe3O4@ß-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components. The introduction of rGO in the composites was beneficial to the generation of •OH, and the role of ß-CD might enhance the utilization of •OH. A possible three-element catalytic schematic diagram was described. The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated. The removal of BPA and TOC retained 78.2 ±â€¯2.4% and 52.9 ±â€¯2.5% after five cycles, indicating its excellent stability and reusability. Furthermore, a probable reaction pathway of BPA removal was suggested by analyzing the intermediate products. All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.


Assuntos
Compostos Benzidrílicos/química , Catálise , Recuperação e Remediação Ambiental/métodos , Óxido Ferroso-Férrico/química , Grafite/química , Fenóis/química , Fotólise , beta-Ciclodextrinas/química , Peróxido de Hidrogênio/química , Hidroxilamina/química
12.
Shock ; 51(6): 770-779, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30562304

RESUMO

Long noncoding RNAs (lncRNAs), once thought to be transcriptional noise, have been recently shown to regulate a variety of biological processes. However, their roles in the inflammatory response are largely unexplored. In this study, we performed high-throughput sequencing to identify the profiles of mRNA and lncRNA transcriptomes in response to lipopolysaccharide (LPS) stimulation, followed by a comprehensive bioinformatics analysis. We found a total of 325 lncRNAs and 1,187 mRNAs to be significantly dysregulated in RAW264.7 cells stimulated with LPS (fold change >4.0 or <0.25, false discovery rate <0.01). Further validation with qRT-PCR demonstrated that Cd40 and Traf1 mRNAs were significantly upregulated, whereas Slc43a2 and Ccnd1 were downregulated in RAW264.7 cells treated with LPS. Gene ontology (GO) analysis indicated that the altered mRNAs and lncRNAs were mainly involved in the immune response, inflammation response, chemokine receptor binding, protein binding, and regulation of cytokine production. KEGG pathway analysis showed that altered lncRNAs and mRNAs were significantly enriched in immune- and inflammation-related signaling pathways, such as Herpes simplex infection, cytokine-cytokine receptor interaction, and TNF, PI3K-Akt, MAPK, NF-κB, and JAK-STAT signaling pathways. lncRNA-mRNA network analysis showed that the coexpression network profile for mRNAs and lncRNAs from the immune category consisted of 93 network nodes and 145 connections among 70 differentially expressed mRNAs and 23 dysregulated lncRNAs, suggesting that lncRNAs play an important role in the regulation of functional mRNA expression in LPS-induced inflammation.

13.
J Hazard Mater ; 393: 120448, 2019 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32155519

RESUMO

Herein, a novel three-dimensional (3D) heterogeneous Electro-Fenton (EF) system with improved gas diffusion electrode (GDE) as cathode and magnetic nitrogen doped/reduced graphene oxide (Fe3O4/N-rGO) as catalytic particle electrodes (CPEs) was built for Bisphenol A (BPA) removal. The Fe3O4/N-rGO served as both particle electrodes and heterogeneous catalyst. The study concluded that BPA could be effectively removed via this hybrid system. The synergistic effect between the 3D electrode and EF system was discussed by comparing the performance of different functional particle electrodes. The 3D electrode system exhibited a larger specific surface area electrode, which improved the mass transfer of pollutants to electrode, and also accelerated the regeneration of FeⅡ due to faster electron transfer, thereby enhancing the efficiency of EF catalysis. The EF process promotes the regeneration rate of particle electrodes and thus accelerates the 3D electrode reaction course. The parameters affecting degradation behavior of BPA were optimized. As a result, optimal removal rate of BPA and TOC was 93% and 60.5%, respectively within 90 min. The CPEs showed high catalytic performance (86.5% for BPA and 50.3% for TOC) and low catalyst loss (less than 9.5%) after 5 cycles, indicating its excellent stability and reusability. The possible mechanism of 3D heterogeneous EF was investigated by comparing the catalytic activity and •OH production capacity of homogeneous EF and Fenton-like. Built on the analysis of intermediates, a possible decomposition pathway of BPA was proposed.

14.
Ecotoxicol Environ Saf ; 166: 488-497, 2018 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-30312948

RESUMO

An improved method was proposed which integrates the distribution of metal speciation simulated by chemical equilibrium model, different exposure models and average daily intake dose modified by analytic hierarchy process for human health risk assessment of metal species (MS). With the rapid development of economic and urbanization, the metals pollution had become more serious in industrial areas. Adverse effects of soil contaminants on human health in typical industrial area should be assessed to evaluate the risks of soils in these areas. The method was applied to study nickel (Ni) species health risks in soil of industrial areas. The pH possessed significant impact to determine distribution/existence and solubility of Ni species, followed by DOC. The non-carcinogenic risk (HQ) of Ni species were less than 1 in each sampling points, except Ni2+. In addition, the carcinogenic risk (CR) of different Ni species were less than 10-6, except for FANi and Ni2+.


Assuntos
Monitoramento Ambiental/métodos , Poluição Ambiental/análise , Indústrias , Metais Pesados/análise , Medição de Risco/métodos , Poluentes do Solo/análise , Solo/química , China , Humanos , Urbanização
15.
Int J Syst Evol Microbiol ; 68(11): 3494-3499, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30222096

RESUMO

A Gram-stain-negative bacterium, strain BH-SD19T, that was isolated from a marine sediment sample collected from the Bohai Sea, was subjected to a polyphasic taxonomic study. Cells of BH-SD19T are non-flagellated, non-gliding, oval-shaped rods, 0.5-1.0 µm wide and 1.0-2.0 µm long. BH-SD19T is strictly aerobic, and oxidase- and catalase-positive. Growth occurs at 15-40 °C (optimum 35 °C), at pH 6.0-8.5 (optimum 7.0-7.5) and with 1-10 % (w/v) NaCl (optimum 2 %). The predominant fatty acids are C19 : 0cyclo ω8c (46.5 %), C16 : 0 (20.3 %) and C18 : 1ω7c and/or C18 : 1ω6c (10.6 %). The major respiratory quinone is Q-10. The major polar lipids are phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content is 64.0 mol%. BH-SD19T shows the highest 16S rRNA sequence similarity to Pontibaca methylaminivorans (95.2 %) and strains of species of the genus Roseovarius(93.4-95.2 %). Sequence similarity values between BH-SD19T and other phylogenetically related species are all below 95.0 %. Phylogenetic trees based on 16S rRNA gene sequences indicate that BH-SD19T forms a distinct lineage and does not join any known genera in the trees. Phenotypic, chemotaxonomic and phylogenetic data indicate that BH-SD19T represents a novel genus and species in the family Rhodobacteraceae, for which the name Pelagivirga sediminicola gen. nov., sp. nov. is proposed. The type strain is BH-SD19T (=CCTCC AB 2017074T=KCTC 62202T).


Assuntos
Sedimentos Geológicos/microbiologia , Filogenia , Rhodobacteraceae/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Oceanos e Mares , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodobacteraceae/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
16.
J Hazard Mater ; 360: 223-232, 2018 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-30119017

RESUMO

In this research, a novel efficiency MnO2@Nano hollow carbon sphere (MnO2@NHCS) nanocomposite was prepared by one-pot hydrothermal reaction with KMnO4 solution. The adsorption and oxidization performance of MnO2@NHCS were assessed by degradation of bisphenol A (BPA) at different conditions. The effect of dosage of MnO2@NHCS, pH, initial concentration of BPA, temperature and humic acid were investigated systematically. Moreover, the characterizations of MnO2@NHCS were measured by a series of techniques, such as XRD, FESEM, HRTEM, TGA and XPS. Notably, hollow structure of nano carbon sphere was still retained with uniform MnO2 nanosheets covered. The results show that the removal rate of BPA was 95.3% within 10 min and BPA can be almost decomposed in 30 min under the optimal conditions. Additionally, the MnO2@NHCS remained stable and had a high regeneration efficiency (more than 85%) after 3 cycles (360 min). The reaction intermediates/products of oxidation of BPA were analyzed and the possible degradation pathways of BPA were proposed. These research results demonstrate that the MnO2@NHCS is a fleet and efficient material for BPA degradation in aqueous environment.

17.
Int J Oral Sci ; 10(3): 27, 2018 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-30111835

RESUMO

The interplay between mechanoresponses and a broad range of fundamental biological processes, such as cell cycle progression, growth and differentiation, has been extensively investigated. However, metabolic regulation in mechanobiology remains largely unexplored. Here, we identified glucose transporter 1 (GLUT1)-the primary glucose transporter in various cells-as a novel mechanosensitive gene in orthodontic tooth movement (OTM). Using an in vivo rat OTM model, we demonstrated the specific induction of Glut1 proteins on the compressive side of a physically strained periodontal ligament. This transcriptional activation could be recapitulated in in vitro cultured human periodontal ligament cells (PDLCs), showing a time- and dose-dependent mechanoresponse. Importantly, application of GLUT1 specific inhibitor WZB117 greatly suppressed the efficiency of orthodontic tooth movement in a mouse OTM model, and this reduction was associated with a decline in osteoclastic activities. A mechanistic study suggested that GLUT1 inhibition affected the receptor activator for nuclear factor-κ B Ligand (RANKL)/osteoprotegerin (OPG) system by impairing compressive force-mediated RANKL upregulation. Consistently, pretreatment of PDLCs with WZB117 severely impeded the osteoclastic differentiation of co-cultured RAW264.7 cells. Further biochemical analysis indicated mutual regulation between GLUT1 and the MEK/ERK cascade to relay potential communication between glucose uptake and mechanical stress response. Together, these cross-species experiments revealed the transcriptional activation of GLUT1 as a novel and conserved linkage between metabolism and bone remodelling.


Assuntos
Transportador de Glucose Tipo 1/genética , Técnicas de Movimentação Dentária , Ativação Transcricional , Animais , Fenômenos Biomecânicos , Western Blotting , Remodelação Óssea/efeitos dos fármacos , Células Cultivadas , Transportador de Glucose Tipo 1/antagonistas & inibidores , Humanos , Hidroxibenzoatos/farmacologia , Imuno-Histoquímica , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Osteoprotegerina/metabolismo , Ligamento Periodontal/citologia , Ligante RANK/metabolismo , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase Reversa
18.
Mol Microbiol ; 109(4): 494-508, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29923654

RESUMO

Deletion of Prp4, the only kinase among spliceosome components, is not lethal in Fusarium graminearum but Fgprp4 mutants have severe growth defects and produced spontaneous suppressors. To identify novel suppressor mutations of Fgprp4, we sequenced the genome of suppressor S37 that was normal in growth but only partially recovered for intron splicing and identified a tandem duplication of 9-aa in the tri-snRNP component FgSNU66. Among the 19 additional suppressor strains found to have mutations in FgSNU66 (out of 260 screened), five had the same 9-aa duplication event with S37 and another five had the R477H/C mutation. The rest had nonsense or G-to-D mutations in the C-terminal 27-aa (CT27) region of FgSnu66, which is absent in its yeast ortholog. Truncation of this C-terminal region reduced the interaction of FgSnu66 with FgHub1 but increased its interaction with FgPrp8 and FgPrp6. Five phosphorylation sites were identified in FgSnu66 by phosphoproteomic analysis and the T418A-S420A-S422A mutation was shown to reduce virulence. Overall, our results showed that mutations in FgSNU66 can suppress deletion of Fgprp4, which has not been reported in other organisms, and the C-terminal tail of FgSnu66 plays a role in its interaction with key tri-snRNP components during spliceosome activation.


Assuntos
Proteínas Fúngicas/genética , Fusarium/crescimento & desenvolvimento , Fusarium/genética , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Ribonucleoproteínas Nucleares Pequenas/genética , Ribonucleoproteínas Nucleares Pequenas/metabolismo , Supressão Genética/genética , Sequência de Aminoácidos , Sequência de Bases , Proteínas Fúngicas/metabolismo , Deleção de Genes , Genoma Fúngico/genética , Processamento de RNA/genética
19.
Environ Pollut ; 241: 887-899, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29920467

RESUMO

A method was proposed to evaluate comprehensive effects of pHs and total metal concentration (TMC) variation for metal speciation human health risk in groundwater. The method used for the health assessment considered comprehensive and mutative effects caused by oral ingestion of groundwater based on human health risk assessment model and MINTEQ simulation. The results demonstrated that the dissolution rate of Ni2+ was affected by pH and Ni total concentration (total-Ni). With the increase of pH, the Ni2+ dissolved rate was smaller in the higher total-Ni at same pH. Ni2+ was dominant components contributed to health risk in groundwater. With the increase of pH in various total-Ni, HINi keep constant at first, and then decreased gradually. The HINi values of Ni speciation above acceptable level only in high total-Ni with alkaline conditions. The obtained results to verify that metals speciation were determined in health risk, and variation factors (pH and metal total concentration) played important role in risk estimation. These results provide basic information of heavy metal pollution control as well as remediation management.


Assuntos
Monitoramento Ambiental/métodos , Água Subterrânea/química , Metais Pesados/análise , Poluição Ambiental , Humanos , Medição de Risco/métodos
20.
J Hazard Mater ; 354: 170-179, 2018 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-29751173

RESUMO

In China, potential heavy metal hazard around abandoned lead-acid battery plant (ALBP) area has been a great concern but without detailed report. The distribution and sources of heavy metals in soils and so by risk assessment associated with ALBP are conducted in this contribution, based on geographies and statistics. Pb and Zn are quantitively identified to be still emitted from ALBP soil, and Cd as well As are from agricultural activity. We investigate vertical metal distribution, and fortunately find that metals migrate within limit of 40 cm below topsoil, which is higher than groundwater table. The visualized stable depths are Zn 40 cm, Pb, As 20 cm, and Cd 40 cm. The mapped pollution load index (PLI) suggests a high pollution level exists in ALBP soil. The estimation of potential ecological risk index (PERI) indicates a light ecological risk in studied area, while As and Cd mainly from agricultural activity possess 54% of total Eri. Health risk index (THI) is 0.178 for children, indicating non-cancer risks may be ignored in observed area. Though calculated risk is temporarily affordable, soil remediation and reduction of agricultural chemical reagents are recommended for preventing potential cumulative risk from further bioconcentration of heavy metals.

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