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1.
J Pharm Pharmacol ; 2020 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-32419149

RESUMO

OBJECTIVES: Eurycoma longifolia Jack (Simaroubaceae) is commonly distributed in the Southeast Asia and Indo China, which has been shown to possess antianxiety, antibacterial, anticancer, antifungal, anti-inflammatory, antimalarial and antioxidant biological activities. 14,15ß-dihydroxyklaineanone is a diterpene isolated from E. longifolia Jack, which is cytotoxic against human lung cancer and human breast cancer cell lines. However, the effects and underlying mechanisms of 14,15ß-dihydroxyklaineanone on hepatocellular carcinoma remain unknown. METHODS: Cell viability assay and colony formation assay were used to measure HepG2 cell proliferation. Flow cytometry was used to analyse cell cycle and apoptosis. Wound-healing assay and transwell assay were used to observe cells migration. RNA sequencing and the enrichment of differentially expressed genes (DEGs) in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were used to find and determine underlying pathways. KEY FINDINGS: We found that 14,15ß-dihydroxyklaineanone inhibited the growth and migration of HepG2 cells but did not induce cell apoptosis. 14,15ß-dihydroxyklaineanone induced S cell cycle arrest by downregulating the expression levels of cyclin A, p-CDK2, cyclin B1, p21, E2F-1 and PCNA. In addition, RNA sequencing showed that 14,15ß-dihydroxyklaineanone regulated MAPK pathway by increasing the expression levels of phosphor-p38. Downregulating of p38 via both p38 inhibitor (SB203580) and p38-siRNA could antagonize the inhibition of cell proliferation and migration and reverse the changes in p-p38, E-cadherin, N-cadherin and PCNA expression induced by 14,15ß-dihydroxyklaineanone treatment. CONCLUSIONS: 14,15ß-dihydroxyklaineanone inhibited cell proliferation and migration through regulating p38 MAPK pathway in HCC cells.

2.
Biochem Pharmacol ; 175: 113849, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32059841

RESUMO

Gestational diabetes mellitus (GDM) is associated with an increased risk of progressing to type 2 DM and cardiovascular disease; however, the pathogenesis is still poorly understood. This study was to investigate roles of thrombin and its receptor protease-activated receptor 1 (PAR-1) and NLRP1 inflammasome in endothelial injury in GDM condition. Umbilical cord and plasma of GDM patients and high glucose (HG) cultured human umbilical vein endothelial cells (HUVECs) were used to examine the pathological changes of these pathways. Meanwhile, ameliorative effects and potential mechanisms of a natural product sarsasapogenin (Sar) were investigated in HUVECs. Thrombin/PAR-1 pathway, advanced glycation endproducts (AGEs) and their receptor (RAGE) axis, and the nucleotide-binding domain and leucine-rich repeat containing protein 1 (NLRP1) inflammasome were activated in GDM condition and HG-cultured HUVECs, accompanied by endothelial injury (decreased cell viability and increased lactate dehydrogenase release). Nevertheless, thrombin inhibition or PAR-1 antagonism caused decreases in AGEs formation and RAGE expression in HG-cultured HUVECs, while AGEs inhibition or RAGE antagonism declined PAR-1 expression not thrombin activity. Furthermore, thrombin inhibition or PAR-1 antagonism restrained NLRP1 inflammasome activation in HG-cultured HUVECs; meanwhile, NLRP1 expression and interleukin 18 levels were remarkably reduced in HG-cultured HUVECs after PAR-1 knockdown. Interestingly, Sar co-treatment could suppress thrombin/PAR-1 pathway, NLRP1 inflammasome, and AGEs/RAGE axis. Together, endothelial damages in GDM were likely due to enhanced interaction between AGEs/RAGE axis and thrombin/PAR-1 pathway, followed by NLRP1 inflammasome activation. Moreover, Sar may act as a protective agent against endothelial injury in chronic HG condition.

3.
Plant Sci ; 291: 110346, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31928677

RESUMO

NAC (NAM, ATAF1/2 and CUC2) proteins are plant-specific transcription factors (TFs) that are important in plant abiotic stress responses. In this study we isolated a NAC gene from Capsicum annuum leaves, designated as CaNAC064. We characterized the amino acid sequence of CaNAC064 and found that it contain conserved domains of the NAC family, including a highly conserved N-terminus domain and a highly variable C-terminus domain. Expression analysis showed that the 40C, 400C, salicylic acid (SA) and abscisic acid (ABA) treatments strongly induced the expression of CaNAC064 through silencing of CaNAC064 in pepper and overexpressing in Arabidopsis. CaNAC064-silenced pepper plants exhibited more serious wilting, higher MDA contents and chilling injury index, lower proline content, and more accumulation of ROS in the leaves after cold stress. The CaNAC064-overexpressing Arabidopsis plants exhibited lower MDA content, chilling injury index and relative electrolyte leakage content as compared to WT plants under cold stress. Transcriptional activation activity analysis indicated that CaNAC064 has transcriptional activation activity in the 691-1071 bp key region. We identified 45 proteins that putatively interact with CaNAC064 using the Yeast Two-Hybrid method. According to the Yeast Two-Hybrid and BIFC results, CaNAC064 interacted with low temperature-induced haplo-proteinase proteins in plant cell. These results suggested that CaNAC064 positively modulates plant cold-tolerance, laying the foundation for future investigations into the role of NACs as regulatory proteins of cold tolerance in plants.

4.
Huan Jing Ke Xue ; 40(10): 4423-4430, 2019 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854809

RESUMO

A series of MgAl hydrotalcite-derived composite oxides were prepared by co-precipitation methods. The effects of calcination temperature, reaction temperature, water vapor volume fraction, and alkali metal addition on the hydrolysis activity of the samples were investigated. The crystal structure, specific surface area, pore structure, and basic position distribution of the composite oxides were characterized using XRD, BET, TPD, and XPS. The results shown that the catalytic activity firstly increased and then decreased with the rise of calcination temperature. Furthermore, the sample that calcined at 650℃ can achieve the best catalytic activity (complete removal of COS lasted for 180 min). Increasing the reaction temperature improved the catalytic activity, which can present excellent catalytic activity and stability at temperatures above 70℃. In addition, the doping of the alkali metal Cs improved the catalytic activity, the complete removal time for COS can be maintained for 480 min.

5.
Int J Mol Sci ; 21(1)2019 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-31861623

RESUMO

Dehydrins play an important role in improving plant resistance to abiotic stresses. In this study, we isolated a dehydrin gene from pepper (Capsicum annuum L.) leaves, designated as CaDHN4. Sub-cellular localization of CaDHN4 was to be found in the nucleus and membrane. To authenticate the function of CaDHN4 in cold- and salt-stress responses and abscisic acid (ABA) sensitivity, we reduced the CaDHN4 expression using virus-induced gene silencing (VIGS), and overexpressed the CaDHN4 in Arabidopsis. We found that silencing of CaDHN4 reduced the growth of pepper seedlings and CaDHN4-silenced plants exhibited more serious wilting, higher electrolyte leakage, and more accumulation of ROS in the leaves compared to pTRV2:00 plants after cold stress, and lower chlorophyll contents and higher electrolyte leakage compared to pTRV2:00 plants under salt stress. However, CaDHN4-overexpressing Arabidopsis plants had higher seed germination rates and post-germination primary root growth, compared to WT plants under salt stress. In response to cold and salt stresses, the CaDHN4-overexpressed Arabidopsis exhibited lower MDA content, and lower relative electrolyte leakage compared to the WT plants. Under ABA treatments, the fresh weight and germination rates of transgenic plants were higher than WT plants. The transgenic Arabidopsis expressing a CaDHN4 promoter displayed a more intense GUS staining than the normal growth conditions under treatment with hormones including ABA, methyl jasmonate (MeJA), and salicylic acid (SA). Our results suggest that CaDHN4 can protect against cold and salt stresses and decrease ABA sensitivity in Arabidopsis.


Assuntos
Ácido Abscísico/farmacologia , Arabidopsis/crescimento & desenvolvimento , Capsicum/metabolismo , Proteínas de Plantas/metabolismo , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Capsicum/genética , Núcleo Celular/metabolismo , Resposta ao Choque Frio , Citoplasma/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Germinação/efeitos dos fármacos , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas/efeitos dos fármacos , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Espécies Reativas de Oxigênio/metabolismo , Estresse Salino
6.
Artigo em Inglês | MEDLINE | ID: mdl-31702523

RESUMO

Due to their unique properties, nanofibers have been widely used in various areas, for example, information industry, pharmaceutical application, environmental industry, textile and clothing, etc. Bubble electrospinning is one of the most important non-needle electrospinning methods for nanofiber fabrication. It usually uses polymers' bubbles for production of nanomaterials by using electrostatic force, flowing air or mechanical force to overcome the surface tension of bubbles. Bubble electrospinning mainly includes the bubble electrospinning and blown bubble electrospinning. A history of the development of bubble electrospinning is briefly introduced in this article. The methods of bubble electrospinning are mainly included single bubble electrospinning, porous bubble electrospinning, blown bubble electrospinning, electrostatic-field-assisted blown bubble spinning and others. These different bubble electrospinning methods are also discussed in this paper.

7.
Environ Toxicol Pharmacol ; 68: 13-18, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30852303

RESUMO

Alternate forms of drug crystals display different physicochemical properties. These include stability, dissolution rate, bioavailability and solubility, which can affect pharmacokinetics and pharmacodynamics. It is therefore important to compare the crystal forms of cedrol to obtain optimal anti-inflammatory and analgesic effects. This study, for the first time, obtained and reports three novel forms (I-III) of cedrol polymorphs. The three forms of cedrol were recrystallized from seven organic solvents by slow cooling or volatilization and identified by thermal analysis, fourier transform infrared spectroscopy, scanning electron microscopy and powder X-ray diffraction analysis. Form I originated from acetone and cyclohexane. Form II was obtained from ethanol, ethyl acetate, acetonitrile and n-hexane. Form III was recrystallized from methanol. The anti-inflammatory and analgesic activities of the three crystalline forms were evaluated by acetic acid induced writhing in mice, the hot plate method, carrageenan induced mouse paw edema models, Xylene-induced mouse ear edema models and cotton pellet-induced mouse granuloma models. Experimental results revealed that the highest performance was achieved from Form I. These findings are of great significance during the early research study of cedrol polymorphs.


Assuntos
Analgésicos/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Edema/tratamento farmacológico , Granuloma/tratamento farmacológico , Dor/tratamento farmacológico , Terpenos/uso terapêutico , Ácido Acético , Animais , Carragenina , Fibra de Algodão , Edema/induzido quimicamente , Feminino , Temperatura Alta , Camundongos , Dor/induzido quimicamente , Xilenos
8.
Biomed Pharmacother ; 111: 1166-1175, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30841430

RESUMO

Diabetic nephropathy (DN) is one of the most common diabetic complications, and alpha-carbonyl aldehydes and their detoxicating enzyme glyoxalase 1 (Glo-1) play vital roles in pathogenesis of diabetic complications. The aim of this study was to evaluate the renoprotective effects of hesperetin against DN in rats, and to investigate mechanisms from the aspect of Nrf2/ARE/Glo-1 pathway. Streptozotocin-induced diabetic rats were treated orally with hesperetin (50 and 150 mg/kg), or nuclear factor erythroid-derived-2-like 2 (Nrf2) inducer tert-butylhydroquinone (tBHQ, 25 mg/kg) for 10 weeks. Then proteinuria, creatinine, urea nitrogen, and uric acid were assayed for renal functions, fibronectin and collagen IV levels by immunohistochemistry, as well as periodic acid-Schiff staining and electron microscope observation, were used to assess renal morphology. Glo-1 activity, protein, and mRNA levels and the classic Nrf2/ARE pathway were investigated. Moreover, advanced glycation endproducts (AGEs) and its receptor RAGE, interleukin-1ß and tumor necrosis factor-α levels were also examined in the kidney. Hesperetin markedly ameliorated the renal functions and structural changes of diabetic rats, accompanied by up-regulation of Glo-1 as well as inhibition of AGEs/RAGE axis and inflammation. Meanwhile, hesperetin caused significant increases in Nrf2 and p-Nrf2 levels, as well as up-regulation of γ-glutamylcysteine synthetase, a well-known target gene of Nrf2/ARE signaling. Our results demonstrated that hesperetin could slow down the pathological process of DN, and Glo-1 enhancement contributed to the beneficial effects, which was obtained by the activation of Nrf2/ARE pathway.


Assuntos
Hidrolases de Éster Carboxílico/metabolismo , Nefropatias Diabéticas/tratamento farmacológico , Hesperidina/farmacologia , Lactoilglutationa Liase/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Transdução de Sinais/efeitos dos fármacos , Animais , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Experimental/metabolismo , Nefropatias Diabéticas/etiologia , Nefropatias Diabéticas/metabolismo , Glutamato-Cisteína Ligase/metabolismo , Produtos Finais de Glicação Avançada/metabolismo , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Rim/efeitos dos fármacos , Rim/metabolismo , Masculino , Substâncias Protetoras/farmacologia , Ratos , Ratos Sprague-Dawley , Estreptozocina/farmacologia , Regulação para Cima/efeitos dos fármacos
9.
Ying Yong Sheng Tai Xue Bao ; 29(7): 2269-2276, 2018 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-30039665

RESUMO

An experiment was conducted to examine the differences of soil microbial diversity across different land use patterns in montane region of eastern Liaoning Province, China. The relationships between soil physicochemical properties and soil microbial diversity in five different land use types, including Quercus mongolic forest, shrubland, Larix gmelinii plantation, Pinus koraiensis plantation, and Zea mays cropland were analyzed by Biolog-Eco method. The results showed that both soil total C and N contents were the highest in the Q. mongolica forest, which were 57.74 and 4.40 g·kg-1, followed by shrubland, but only 17.46 and 1.31 g·kg-1 in the Z. mays cropland, respectively. There were significant differences in microbial utilization rate of different land use types. The carbon utilization capacity by soil microbial communities was following the order of Q. mongolica forest > shrubland > L. gmelinii plantation > P. koraiensis plantation > Z. mays cropland, indicating that soil microbial metabolism and activity in Z. mays cropland were the lowest. The Shannon diversity index (2.997), Simpson diversity index (0.942) and McIntosh diversity index (5.256) of soil microbial community in the Z. mays cropland were significantly lower than those in other ecosystems. The average absorbance value (AWCD) was associated with Simpson diversity index and McIntosh diversity index. Esters, alcohols and amines were the primary carbon sources for the differentiation, which might be due to a joint action of many factors such as litter, soil nutrients, and specific soil microorganisms. The soil nutrient and soil microbial community diversity in forest land after reclamation sharply decreased, causing the loss of soil fertility and productivity. The region should keep the Q. mongolica forest, which could help restore soil fertility.


Assuntos
Agricultura , Ecossistema , Microbiologia do Solo , Carbono , China , Florestas , Solo
10.
Front Microbiol ; 8: 2189, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29163457

RESUMO

The opportunistic pathogen Staphylococcus aureus has become an increasing threat to public health. While the Agr quorum sensing (QS) system is a master regulator of S. aureus virulence, its dysfunction has been frequently reported to promote bacteremia and mortality in clinical infections. Here we show that the Agr system is involved in persister formation in S. aureus. Mutation of either agrCA or agrD but not RNAIII resulted in increased persister formation of stationary phase cultures. RNA-seq analysis showed that in stationary phase AgrCA/AgrD and RNAIII mutants showed consistent up-regulation of virulence associated genes (lip and splE, etc.) and down-regulation of metabolism genes (bioA and nanK, etc.). Meanwhile, though knockout of agrCA or agrD strongly repressed expression of phenol soluble modulin encoding genes psmα1-4, psmß1-2 and phenol soluble modulins (PSM) transporter encoding genes in the pmt operon, mutation of RNAIII enhanced expression of the genes. We further found that knockout of psmα1-4 or psmß1-2 augmented persister formation and that co-overexpression of PSMαs and PSMßs reversed the effects of AgrCA mutation on persister formation. We also detected the effects on persister formation by mutations of metabolism genes (arcA, hutU, narG, nanK, etc.) that are potentially regulated by Agr system. It was found that deletion of the ManNAc kinase encoding gene nanK decreased persister formation. Taken together, these results shed new light on the PSM dependent regulatory role of Agr system in persister formation and may have implications for clinical treatment of MRSA persistent infections.

11.
World J Urol ; 33(12): 2169-75, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25774006

RESUMO

PURPOSE: To report the clinical features of pelvic fracture urethral injury (PFUI) and assess the real effect of factors that are believed to have adverse effects on delayed urethroplasty. METHODS: An observational descriptive study in a single urological center examined 376 male patients diagnosed with PFUI who underwent open urethroplasty from 2009 to 2013. Analyzed factors included patient age at the time of injury, etiology of PFUI, type of emergency treatment, concomitant injuries, length and position of stricture, type of urethroplasty and the outcome of surgery. Univariate and multivariate logistic regression analyses were applied, together with analytical statistic methods such as t test and Chi-square test. RESULTS: The overall success rate of delayed urethroplasty was 80.6 %. Early realignment was associated with reduced stricture length and had beneficial effect on delayed surgery. Concomitant rectum rupture, strictures longer than 1.6 cm and strictures closer than 3 cm to the bladder neck were indicators of poor outcome. Age, type of injury, urethral fistula and bladder rupture were not significant predicators of surgery outcome. Failed direct vision internal urethrotomy and urethroplasty had no significant influence on salvage operation. CONCLUSIONS: The outcome of posterior urethroplasty is affected by multiple factors. Early realignment has beneficial effect; while the length and position of stricture and its distance to bladder neck plays the key role, rectum rupture at the time of injury is also an indicator of poor outcome. The effect of other factors seems insignificant.


Assuntos
Fraturas Ósseas/complicações , Ossos Pélvicos/lesões , Procedimentos Cirúrgicos Reconstrutivos , Centros de Atenção Terciária , Uretra/lesões , Uretra/cirurgia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , China , Fraturas Ósseas/cirurgia , Hospitais com Alto Volume de Atendimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
12.
Huan Jing Ke Xue ; 33(10): 3529-34, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23233984

RESUMO

The cultivation and stability of aerobic granular sludge in a three sequencing airlift internal-loop aerobic granular fluidized beds (R1-R3) under different organic loading rates (OLR) were investigated, where the selective pressure was un-controlled. R1 and R2 were start-up at the COD loading of 7 kg x (m3 x d)(-1) and 3 kg x (m3 x d)(-1) respectively, and R3 was start-up at an increasing COD loading rates of 1.5-3 kg x (m3 x d)(-1). The results showed that the aerobic granules could be formed successfully in all the reactors, however, filamentous bulking happened as the reactor was start-up at an aimed OLR (R1 and R2). It seems the overgrowth of filamentous could be controlled effectively by means of increasing OLR gradually. The granular development characteristics, the physical characteristics and extracellular polymeric substances contents were analyzed especially during the aerobic granules cultivation. Compared with the granules in R1 and R2, aerobic granules formed in R3 presented clearer outer morphology and compact structure, advanced COD removal efficiency and a significant increase in polysaccharides, resulted an enhanced stability.


Assuntos
Reatores Biológicos , Compostos Orgânicos/metabolismo , Esgotos/microbiologia , Eliminação de Resíduos Líquidos/métodos , Aerobiose , Reatores Biológicos/microbiologia , Compostos Orgânicos/isolamento & purificação , Tamanho da Partícula
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