Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 9 de 9
Filtrar
Mais filtros










Base de dados
Tipo de estudo
Intervalo de ano de publicação
1.
J Cell Biochem ; 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31452277

RESUMO

MiRNAs were involved in the various biological process through mediating the posttranscriptional gene silencing. The abnormal expression of miRNAs is also involved in various disorders. Our previous study showed that miRNA-27a (miR-27a) was upregulated after ultraviolet B (UVB) irradiation. However, the function of miR-27a in UVB-induced cell damage is still unclear. In this study, we used the miR-27a overexpression and knockdown lentivirus to transfect UVB irradiated HaCaT cell line and observed the influence of miR-27a on UVB irradiated damages in cells. We found that miR-27a removed cyclobutane pyrimidine dimers (CPDs) and decreased the cell apoptosis after UVB radiation. Further studies showed that miR-27a directly decreased the expression and luciferase activity of target genes transactive response DNA-binding protein (TARDBP) and apoptotic protease activating factor-1 (APAF-1). In conclusion, miR-27a can inhibit CPDs, reduce the cell apoptosis and down-regulate its target genes TARDBP and APAF-1 induced by UVB irradiation in HaCaT cells. It is indicated that miR-27a may serve as a target for UVB irradiation protection.

2.
Arch Dermatol Res ; 311(4): 277-285, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30826962

RESUMO

To verify whether PsA-associated HLA alleles proposed in other populations are also related to PsA in Chinese Han population, a study of PsA susceptible alleles in the HLA-A, HLA-B, HLA-C and HLA-DRB1 alleles was presented for Chinese Han population. Genotyping was performed by Illumina Miseq platform (Illumina, USA). 50 subtypes and 77 subtypes of HLA-A, HLA-B, HLA-C and HLA-DRB1 with minor allele frequency (MAF) > 1% were genotyped from two-digit and four-digit resolution analysis in 111 PsA and 207 HCs (healthy controls) collected from Chinese Han population, respectively. Data handling, quality control and association analysis were performed using SPSS 25.0 software. In risk estimate, by mean of Bonferroni correction, a newfound four-digit allele HLA-A*01:01 [P = 5.5 × 10-4, OR 3.35 (1.69-6.66)], four-digit allele HLA-C*06:02 [P = 8.5 × 10-7, OR 3.80 (2.23-6.47)] and six two-digit alleles HLA-A*01 [P = 5.2 × 10-5, OR 3.43 (1.89-6.23)], HLA-B*13 [P = 4.0 × 10-6, OR 2.65 (1.76-4.01)], HLA-B*27 [P = 7.5 × 10-4, OR 5.84 (2.09-16.29)], HLA-B*57 [P = 5.8 × 10-5, OR 20.10 (4.65-86.83)], HLA-C*03 [P = 2.1 × 10-4, OR 0.40 (0.25-0.65)], HLA-C*06 [P = 1.9 × 10-12, OR 4.48 (2.95-6.81)] showed statistical significance by the univariate binary logistic regression analysis. Besides, in the binary logistic regression analysis with multiple variables, when the two alleles HLA-A*01:01 and HLA-C*06:02 were considered as covariates, HLA-A*01:01 [P = 2.7 × 10-3,OR 2.95 (1.46-5.98)] also showed significant association for PsA as risk factor, but may be not the main risk factor [HLA-C*06:02, P = 3.0 × 10-6, OR 3.68 (2.13-6.37)]. When all the above two-digit alleles were included as covariates, HLA-A*01 [P = 4.8 × 10-2, OR 2.00 (1.01-3.94)], HLA-B*13 [P = 4.2 × 10-5, OR 2.62 (1.65-4.16)], HLA-B*27 [P = 1.7 × 10-4, OR 7.62 (2.64-21.96)], HLA-B*57 [P = 2.97 × 10-4, OR 15.90 (3.55-71.18)], HLA-C*06 [P = 6.1 × 10-5, OR 2.70 (1.66-4.40)] showed significant for PsA as risk factors, HLA-C*03 [OR 0.65 (0.39-1.09), P = 0.10] showed no association with PsA. In conclusion, we assessed HLA-A, HLA-B, HLA-C and HLA-DRB1 alleles in PsA cohort of Chinese Han population, found HLA-A*01:01 and HLA-A*01 may be the susceptible genes associated with PsA, and also confirmed the association of four loci with PsA in Chinese Han population. These findings may extend the susceptibility HLA alleles of PsA and help in developing possible genetic markers to predict PsA.


Assuntos
Artrite Psoriásica/genética , Genótipo , Antígeno HLA-A1/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , China , Estudos de Coortes , Feminino , Frequência do Gene , Estudos de Associação Genética , Marcadores Genéticos , Predisposição Genética para Doença , Humanos , Complexo Principal de Histocompatibilidade/genética , Masculino , Pessoa de Meia-Idade , Polimorfismo Genético , Risco , Adulto Jovem
3.
JAMA Dermatol ; 155(3): 327-334, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30698628

RESUMO

Importance: It is necessary to determine whether psoriasis responds to methotrexate in the same manner in patients with and without psoriatic arthritis. Objective: To evaluate the effectiveness and safety of methotrexate in treating patients with psoriasis with and without psoriatic arthritis. Design, Setting, and Participants: In this prospective, single-arm, interventional study, a total of 235 patients with psoriasis, 107 without psoriatic arthritis and 128 with psoriatic arthritis who were receiving methotrexate therapy from April 1, 2015, to December 31, 2017, were recruited from the outpatient department of a hospital at a large Chinese university. There were no significant demographic or clinical differences between the subgroups with the exception of diabetes. Interventions: A 12-week course of low-dosage oral methotrexate (7.5-15 mg weekly). Main Outcomes and Measures: Changes in disease severity, adverse events, blood cell counts, and liver and renal function. Results: A total of 235 patients with psoriasis (166 male [66.0%]; mean [SD] age, 49.6 [15.1] years) received methotrexate treatment for 12 weeks. The 90% reduction from baseline Psoriasis Area Severity Index response was significantly lower in patients with psoriatic arthritis than in patients without psoriatic arthritis at week 8 (4 0f 128 [3.1%] vs 12 of 107 [11.2%]; P = .02) and week 12 (19 of 128 [14.8%] vs 27 of 107 [25.2%]; P = .049). Furthermore, the incidence of adverse events, including dizziness (12 of 128 [9.4%] vs 1 of 107 [0.9%]; P = .007), gastrointestinal symptoms (32 of 128 [25.0%] vs 13 of 107 [12.1%]; P = .01), and hepatoxicity (34 of 128 [26.6%] vs 16 of 107 [15.0%]; P = .04), was significantly higher in patients with psoriatic arthritis than in patients without psoriatic arthritis. Methotrexate-induced elevation of alanine aminotransferase levels was associated with body mass index (mean [SD] body mass index, 26 [4] in patients with [P = .04] vs 26 [4] in those without [P = .005] psoriatic arthritis) and smoking (17 of 34 [50.0%] in patients with [P = .02] vs 9 of 16 [56.3%] in those without [P = .04] psoriatic arthritis). Conclusions and Relevance: In this study, methotrexate was well tolerated and effective in treating psoriasis. It was more effective, with fewer adverse effects, in patients with psoriasis who did not have psoriatic arthritis than in patients who presented with both psoriasis and psoriatic arthritis. Therefore, methotrexate can be recommended as first-line treatment for psoriasis without arthritis.

4.
J Dermatol Sci ; 91(3): 285-291, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29914851

RESUMO

BACKGROUND: Recent studies have revealed that DNA methylation (DNAm) could modulate gene expression in psoriasis (Ps). However, the relationship between whole-genome DNAm and gene expression in Ps has not been studied yet. OBJECTIVES: To better characterize the relationship between DNAm and gene expression, and to identify biological pathways triggered by changes in methylation involved in the pathogenesis of Ps. METHODS: Differentially methylated sites (DMSs) and differentially expressed genes (DEGs) were analysed by comparing 20 involved psoriatic (PP) skin, 20 uninvolved psoriatic (PN) skin and 20 normal (NN) skin biopsies. DEGs in negative correlation with the methylation were entered into further Gene Ontology (GO) and pathway analysis by clusterProfiler package in R program. RESULTS: A total of 290 genes with reverse correlation overlapped in PP vs PN and PP vs NN comparisons. GO categories of reversely-associated genes mainly enriched in T cell activation, type I interferon signaling pathway and defense response to other organism. Pathway analysis revealed superior NOD-like receptor signaling pathway and Measles enriched in the differentially up-regulated transcripts and regulation of lipolysis in adipocytes in the down-regulated transcripts. CONCLUSIONS: Our results provided a comprehensive correlation analysis of transcriptome and methylome in Ps. Increased innate immunity and decreased lipid biosynthesis play important roles in the development of psoriatic skin. This integrated analysis shed light on novel insights into the pathogenic mechanisms involved in Ps.


Assuntos
Metilação de DNA , Epigênese Genética , Perfilação da Expressão Gênica/métodos , Psoríase/genética , Transcriptoma , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China/epidemiologia , Biologia Computacional , Bases de Dados Genéticas , Redes Reguladoras de Genes , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Imunidade Inata/genética , Lipogênese/genética , Fenótipo , Psoríase/etnologia , Psoríase/imunologia , Psoríase/metabolismo , Pele/imunologia , Pele/metabolismo
5.
Environ Sci Pollut Res Int ; 25(17): 16702-16709, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29611121

RESUMO

In the Hebei Plain of North China, 70% of the inhabitants depend on groundwater for drinking water. Little is known regarding high-iodine concentrations in groundwater because there have been few systematic studies on high levels of iodine in groundwater in this region. To help identify the mechanisms that control the spatial distribution of iodine in groundwater, 61 samples of shallow groundwater and 161 samples of deep groundwater were collected along a sample section from Taihang Mountain to the Bo Sea. There were four pockets of high-iodine concentrations along the sample section. As the groundwater depth increased, the ratio of undetected iodine decreased, and the ratio of high-iodine concentrations increased. The high-iodine concentrations in the groundwater reflect the geological and sedimentary settings, and were mainly controlled by pH and Eh. Iodine concentrations were particularly high when the pH was between 7.3 and 8.5, and there was an inflection point at 150 µg/L in the curve of the relationship between iodine concentrations and Eh.


Assuntos
Água Subterrânea , Iodo/química , Poluentes Químicos da Água/análise , China , Monitoramento Ambiental , Poluentes Químicos da Água/química
7.
Environ Sci Pollut Res Int ; 24(27): 21750-21760, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28766146

RESUMO

Dissolved organic matter (DOM), as the most active organic carbon in the soil, has a coherent affinity with heavy metals from inherent and exogenous sources. Although the important roles of DOM in the adsorption of heavy metals in soil have previously been demonstrated, the heterogeneity and variability of the chemical constitution of DOM impede the investigation of its effects on heavy metal adsorption onto soil under natural conditions. Fresh DOM (FDOM) and degraded DOM (DDOM) from sugarcane rind were prepared, and their chemical properties were measured by Fourier-transform infrared spectrometry (FTIR), excitation-emission matrix (EEM) fluorescence spectroscopes, nuclear magnetic resonance (NMR), and molecular weight distribution (MWD). They were also used in batch experiments to evaluate their effects on the adsorption of Cu(II) onto farmland red soil. Based on our results, the chemical structure and composition of DDOM greatly varied; compared with FDOM, the C/O ratio (from 24.0 to 9.6%) and fluorescence index (FI) (from 1.4 to 1.0) decreased, and high molecular weight (>10 kDa) compounds increased from 23.18 to 70.51%, while low molecular weight (<3 kDa) compounds decreased from 56.13 to 12.13%; aromaticity and humification degree were markedly enhanced. The discrepancy of FDOM and DDOM in terms of chemical properties greatly influenced Cu(II) adsorption onto red soil by affecting DOM-Cu(II) complex capacity. The FDOM inhibited the adsorption of Cu(II), while DDOM promoted adsorption, which was significantly influenced by soil pH. Maximum adsorption capacity (Q m) was 0.92 and 5.76 mg g-1 in the presence of FDOM and DDOM, respectively. The adsorption process with DDOM could be better described by the Langmuir model, while that with FDOM was better described by the Freundlich model. The impacts caused by the dynamic changes of the chemical properties of DOM under natural conditions should therefore be considered in the risk assessment and remediation of soils contaminated with heavy metals.


Assuntos
Cobre/química , Compostos Orgânicos/química , Saccharum , Poluentes do Solo/química , Solo/química , Adsorção , Metais Pesados/análise
8.
R Soc Open Sci ; 4(11): 170905, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29291084

RESUMO

In this study, Fe(III)-cross-linked chitosan beads (Fe(III)-CBs) were synthesized and employed to explore the characteristics and primary mechanism of their hexavalent chromium (Cr(VI)) adsorption under low concentration Cr(VI) (less than 20.0 mg l-1) and a pH range from 2.0 to 8.0. Batch tests were conducted to determine the Cr(VI) adsorption capacity and kinetics, and the effects of pH and temperature on the adsorption under low concentration Cr(VI) and a pH range from 2.0 to 8.0. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy were employed to explore the characteristics of Fe(III)-CBs and their Cr(VI) adsorption mechanisms. The results show that, unlike the adsorption of other absorbents, the Cr(VI) adsorption was efficient in a wide pH range from 2.0 to 6.0, and well described by the pseudo-first-order model and the Langmuir-Freundlich isotherm model. The capacity of Cr(VI) adsorption by Fe(III)-CBs was as high as 166.3 mg g-1 under temperature 25°C and pH 6.0. The desorption test was also carried out by 0.1 mol l-1 NaOH solution for Fe(III)-CBs regeneration. It was found that Fe(III)-CBs could be re-used for five adsorption-desorption cycles without significant decrease in Cr(VI) adsorption capacity. Ion exchange was confirmed between functional groups (i.e. amino group) and Cr(VI) anions (i.e. [Formula: see text]). The amino-like functional groups played a key role in Cr(VI) distribution on the Fe(III)-CBs surface; Cr(VI) adsorbed on Fe(III)-CBs was partially reduced to Cr(III) with alcoholic group served as electron donor, and then formed another rate-limiting factor. So, Fe(III)-CBs has a good prospect in purifying low concentration Cr(VI) water with a pH range from 2.0 to 6.0.

9.
Huan Jing Ke Xue ; 33(11): 3768-77, 2012 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-23323405

RESUMO

By collecting water and sediment samples from Yangzonghai Lake and analyzing the total amount and speciation of arsenic, the spatial distribution of arsenic in surface water and sediments was analyzed, the current status of arsenic pollution were estimated, the anthropogenic contribution rate and the arsenic reserve in the lake were calculated respectively. Meanwhile, the sources of arsenic were investigated. The results indicated that the total arsenic content in Yangzonghai Lake was 71.96-101.2 microg x L(-1) in April, 2010, and increased slightly with depth. Dissolved arsenic content was 68.14-96.72 microg x L(-1), with As (III) accounting for 32%. The health risk level of arsenic in the water was 4.77 x 10(-4) - 6.66 x 10(-4) a(-1), posing a considerable threat to the surrounding environment. Arsenic content in sediments lied between 6.05-396.49 mg x kg(-1). In sediments at the depths of 0-2, 2-4, 4-6, 6-8 and 8-10 cm, the average arsenic contents were 155.66, 52.01, 29.78, 19.22 and 17.52 mg x kg(-1) respectively. Arsenic in sediments at 0-2 cm had the highest accumulation degree, with the maximum geoaccumulation index up to 5. At the deeper depths, the accumulation degree of arsenic significantly lowered. The sequence of arsenic average contents of seven forms in sediments in the descending order is residual fraction, humic acids fraction, oxide fraction, strong organic fraction, ion exchange fraction, water soluble fraction and carbonate fraction. With increase of sediments depths, the percentage of bioavailable arsenic decreased, and the percentage of residual fraction arsenic increased rapidly. The anthropogenic contribution rate of arsenic in sediments was the highest at 0-2 cm depth, with average of 81.94%. This rate was much lower at the deeper depths. Currently, the total arsenic reserve in water and sediments of Yangzonghai Lake was 70.65 t, of which 82.68% was contributed by human activities. The phosphate fertilizer plant on the south bank made the greatest contribution to arsenic accumulation in Yangzonghai Lake, followed by golf course on the east bank, thermal power station and hot spring on the north bank.


Assuntos
Arsênico/análise , Sedimentos Geológicos/química , Lagos/análise , Poluentes Químicos da Água/análise , China , Monitoramento Ambiental , Fertilizantes , Resíduos Industriais/análise , Indústrias , Centrais Elétricas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA