Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 278
Filtrar
1.
Food Chem ; 411: 135437, 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36701920

RESUMO

Ferritin proteins are promising nano-carriers for bioactive compound delivery. However, the disassembly properties under acidic/alkaline conditions pose risks of cargo leakage. Herein, genipin-mediated chemical crosslinking method was provided as an alternative and effective strategy to construct robust ferritin nanocarrier through controlled-intramolecular conjugation. As indicated by SDS-/Native- PAGE, the crosslinking degree gradually increased with incubating time prolonging. CD results showed that the cross-linking would decrease α-helix content from 78.4 % to 52.7 % upon 6 h incubation. However, TEM images showed that the genipin-modification has subtle influence on its shell-like structure. Remarkably, the cross-linking can be well controlled by intramolecular subunit-subunit conjugation rather than intermolecular conjugation, giving an excellent monodispersity. Importantly, the covalent cross-linking can tight neighboring subunits and inhibit its disassociation, finally inhibiting the leakage of encapsulated-cargos from ferritin cavity under acidic environments. Such findings suggested that the genipin-mediated cross-linking strategy can fabricate robust nano-carriers for bioactive compound delivery.

2.
Health Inf Sci Syst ; 11(1): 7, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36703901

RESUMO

Purpose: The early detection of organ failure mitigates the risk of post-intensive care syndrome and long-term functional impairment. The aim of this study is to predict organ failure in real-time for critical care patients based on a data-driven and knowledge-driven machine learning method (DKM) and provide explanations for the prediction by incorporating a medical knowledge graph. Methods: The cohort of this study was a subset of the 4,386 adult Intensive Care Unit (ICU) patients from the MIMIC-III dataset collected between 2001 and 2012, and the primary outcome was the Delta Sequential Organ Failure Assessment (SOFA) score. A real-time Delta SOFA score prediction model was developed with two key components: an improved deep learning temporal convolutional network (S-TCN) and a graph-embedding feature extraction method based on a medical knowledge graph. Entities and relations related to organ failure were extracted from the Unified Medical Language System to build the medical knowledge graph, and patient data were mapped onto the graph to extract the embeddings. We measured the performance of our DKM approach with cross-validation to avoid the formation of biased assessments. Results: An area under the receiver operating characteristic curve (AUC) of 0.973, a precision of 0.923, a NPV of 0.989, and an F1 score of 0.927 were achieved using the DKM approach, which significantly outperformed the baseline methods. Additionally, the performance remained stable following external validation on the eICU dataset, which consists of 2,816 admissions (AUC = 0.981, precision = 0.860, NPV = 0.984). Visualization of feature importance for the Delta SOFA score and their relationships on the basic clinical medical (BCM) knowledge graph provided a model explanation. Conclusion: The use of an improved TCN model and a medical knowledge graph led to substantial improvement in prediction accuracy, providing generalizability and an independent explanation for organ failure prediction in critical care patients. These findings show the potential of incorporating prior domain knowledge into machine learning models to inform care and service planning. Supplementary Information: The online version of this article contains supplementary material available 10.1007/s13755-023-00210-5.

3.
Adv Mater ; : e2205047, 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36609920

RESUMO

Artificial neuronal devices are critical building blocks of neuromorphic computing systems and currently the subject of intense research motivated by application needs from new computing technology and more realistic brain emulation. Researchers have proposed a range of device concepts that can mimic neuronal dynamics and functions. Although the switching physics and device structures of these artificial neurons are largely different, their behaviors can be described by several neuron models in a more unified manner. In this paper, the reports of artificial neuronal devices based on emerging volatile switching materials are reviewed from the perspective of the demonstrated neuron models, with a focus on the neuronal functions implemented in these devices and the exploitation of these functions for computational and sensing applications. Furthermore, the neuroscience inspirations and engineering methods to enrich the neuronal dynamics that remain to be implemented in artificial neuronal devices and networks towards realizing the full functionalities of biological neurons are discussed. This article is protected by copyright. All rights reserved.

4.
Biomater Sci ; 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36602031

RESUMO

Oncolytic peptides (OLPs) with membranolytic activity show great potential to combat multidrug-resistant cancer cells. Herein, we report a cationic helical oncolytic polypeptide (OLPP) with potent membranolytic activity for cancer therapy. The OLPP was synthesized by ring-opening polymerization of N-carboxyanhydrides (NCAs) and thiol-ene reaction. The OLPP was resistant to protease, showed high cytotoxicity to a series of cancer cells and caused cancer cell necrosis by quickly lysing cancer cell membrane independent of classic death-related intracellular pathways. Intra-tumoral injection of the OLPP effectively suppressed tumor growth in mice through the direct oncolytic effect. The OLPP represents a potential oncolytic chemotherapeutics for cancer therapy.

5.
Mol Carcinog ; 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36692110

RESUMO

Colorectal cancer (CRC) remains one of the most common malignancies worldwide, and liver metastasis represents a considerable challenge during CRC treatment. Aberrant expression of angiopoietin-like protein 3 (ANGPTL3) has been reported in several human cancer types. However, the function and mechanism of ANGPTL3 in CRC remain unclear. In this study, we first explored ANGPTL3 expression profiles in CRC datasets from ONCOMINE and in local samples from patients with CRC. We then elucidated the function of ANGPTL3 via knockdown and overexpression experiments. Bioinformatic analyses were performed to investigate the biological function and associated molecular mechanisms of ANGPTL3 in CRC oncogenesis and development. Finally, a xenograft model of liver metastasis was used to determine the role of ANGPTL3 in CRC metastasis. Our findings indicated that ANGPTL3 expression was upregulated in human CRC tissues, with high ANGPTL3 expression significantly correlated with poor survival of patients with CRC. ANGPTL3 overexpression promoted the proliferation and migration of CRC cells partially through mitogen-activated protein kinase 14 (MAPK14), while ANGPTL3 silencing had the opposite effect. Moreover, ANGPTL3 downregulation suppressed tumor growth and liver metastasis in xenograft mice. Collectively, the results presented here indicate that ANGPTL3 promotes cell proliferation and liver metastasis partly via MAPK14, suggesting that ANGPTL3 plays a tumor-promoting role in CRC progression and thus may represent a therapeutic target for CRC treatment.

6.
J Chromatogr A ; 1687: 463700, 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36508769

RESUMO

In untargeted liquid chromatography‒mass spectrometry (LC‒MS) metabolomics studies, data preprocessing and metabolic pathway recognition are crucial for screening important pathways that are disturbed by diseases or restored by drugs. Here, we collected high-resolution mass spectrometry data of serum samples from 221 coronary heart disease (CHD) patients under two different chromatographic columns (BEH amide and C18 column) and evaluated the three commonly used software programs (XCMS, Progenesis QI, MarkerView) from four aspects (including signal drift, peak number, metabolite annotation and metabolic pathway enrichment). The results showed that the data preprocessed by the three software programs have different degrees of signal drift, but the StatTarget could improve the data quality to meet the data analysis requirement after correction. In addition, XCMS surpassed other software in detection of real chromatographic peaks and Progenesis QI was the best performer in terms of the number of metabolite annotation. XCMS and Progenesis QI showed different performance in pathway enrichment. However, metabolic pathways based on the combination of XCMS and Progenesis QI had a high coincidence with Progenesis QI. In addition, we also reported that C18 and amide columns were highly complementary and have great potential for cooperation in the context of metabolic pathways. In this study, the effects of different chromatographic columns and software pretreatments on metabolomics data were evaluated based on clinical large cohort samples, which will provide a reference for the metabolomics of clinical samples and guide subsequent mechanistic research.


Assuntos
Metabolômica , Software , Humanos , Espectrometria de Massas/métodos , Metabolômica/métodos , Amidas , Redes e Vias Metabólicas
7.
Sci Total Environ ; 863: 160910, 2023 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-36528096

RESUMO

As a unique nanomachine, DNA Walker can move continuously along a specific orbit to amplify signal. Therefore, based on DNA Walker and endonuclease assisted signal amplification strategy, a novel dual-mode visual electrochemical aptasensor was constructed for the detection of Pb2+. Ceric dioxide@mesoporous carbon (CeO2/CS)@AuNPs not only could improve the conductivity of sensing interface but also could fix the aptamer. DNA Walker moved on the surface of the electrode to realize the pairing with the Ag-γFe2O3/cDNA probe, forming a special base sequence that could be spliced by the Nb.BbvCI. Under the action of endonuclease Nb.BbvCI, the Ag-γFe2O3/cDNA probe was continuously sheared and the amount on the electrode was decreased to amplify the signal. Besides, the nanoenzyme of Ag-γFe2O3 could catalyze 3'3'5'5'-tetramethylbenzidine (TMB) to blue color realizing the visual detection of Pb2+. The sensor has been successfully applied to the visual and accurate rapid detection of Pb2+ in aquatic products. The fabricated method of the sensor open up a new way for visual and accurate the detection of environmental pollutants.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Nanopartículas Metálicas , DNA Complementar , Chumbo , Ouro , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Limite de Detecção , Nióbio , DNA/genética , Endonucleases
8.
Plant Physiol Biochem ; 194: 440-448, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36493591

RESUMO

Taraxacum kok-saghyz Rodin (Tk) is a promising alternative rubber-producing grass. However, low biomass and rubber-producing capability limit its commercial application. As a carbon source transporter in plants, sugar will eventually be exported transporters (SWEETs) have been reported to play pivotal roles in diverse physiological events in the context of carbon assimilate transport and utilization. Theoretically, SWEETs would participate in Tk growth, development and response to environmental cues with relation to the accumulation of rubber and biomass, both of which rely on the input of carbon assimilates. Here, we identified 22 TkSWEETs through homology searching of the Tk genomes and bioinformatics analyses. RNA-seq and qRT-PCR analysis revealed these TkSWEETs to have overlapping yet distinct tissue expression patterns. Two TkSWEET isofroms, TkSWEET1 and TkSWEET12 expressed substantially in the latex, the cytoplasm of rubber-producing laticifers as well as the rubber source. As revealed by the transient expression analysis using Tk mesophyll protoplasts, both TkSWEET1 and TkSWEET12 were located in the plasma membrane. Heterologous expressions of the two TkSWEETs in a yeast mutant revealed that only TkSWEET1 exhibited apparent sugar transport activities, with a preference for monosaccharides. Interestingly, TkSWEET12, the latex-predominant TkSWEET isoform, seemed to have evolved from a tandem duplication event that results in a cluster of six TkSWEET genes with the TkSWEET12 therein, suggesting its specialized roles in the laticifers.


Assuntos
Látex , Taraxacum , Borracha/metabolismo , Taraxacum/genética , Taraxacum/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteínas de Membrana Transportadoras/genética , Saccharomyces cerevisiae/metabolismo , Isoformas de Proteínas/metabolismo , Açúcares/metabolismo , Regulação da Expressão Gênica de Plantas
9.
Proc Natl Acad Sci U S A ; 120(1): e2209339120, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36577068

RESUMO

Cephalotaxines harbor great medical potential, but their natural source, the endemic conifer Cephalotaxus is highly endangered, creating a conflict between biotechnological valorization and preservation of biodiversity. Here, we construct the whole biosynthetic pathway to the 1-phenethylisoquinoline scaffold, as first committed compound for phenylethylisoquinoline alkaloids (PIAs), combining metabolic modeling, and transcriptome mining of Cephalotaxus hainanensis to infer the biosynthesis for PIA precursor. We identify a novel protein, ChPSS, driving the Pictet-Spengler condensation and show that this enzyme represents the branching point where PIA biosynthesis diverges from the concurrent benzylisoquinoline-alkaloids pathway. We also pinpoint ChDBR as crucial step to form 4-hydroxydihydrocinnamaldehyde diverging from lignin biosynthesis. The elucidation of the early PIA pathway represents an important step toward microbe-based production of these pharmaceutically important alkaloids resolving the conflict between biotechnology and preservation of biodiversity.


Assuntos
Alcaloides , Benzilisoquinolinas , Cephalotaxus , Cephalotaxus/genética , Biotecnologia
10.
Food Res Int ; 162(Pt B): 112076, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36461393

RESUMO

Sugar, as an essential component of beverages, not only provides sweetness in beverages but also plays a significant role in their flavor, texture, and preservation. In recent years, global sugar consumption has continued to increase, causing a variety of health concerns. Currently, there is growing awareness of the adverse effects of high-sugar consumption. Since beverages are the primary source of daily sugar intake, sugar reduction in beverages is imperative. In this work, the necessity of sugar reduction in beverages was first introduced. Furthermore, four primary sugar reduction strategies (direct sugar reduction, multi-sensory integration, sweeteners, and sweetness enhancers) employed in the beverage industry were systematically summarized. Each sugar reduction strategy was critically compared, while the current research progresses as well as challenges were discussed. The application of sweeteners is the most effective and widely used strategy for sugar reduction in spite of flavor and health concerns of sweeteners. Meanwhile, multi-sensory integration is also a promising strategy for sugar reduction. In addition, different evaluation methods (chemical, cell-based and sensory methods) for sweetness were overviewed. Given the current challenges of sugar reduction, the prospects of sugar reduction in beverages were also discussed. The present work can provide the current progress for sugar reduction in the beverage industry.


Assuntos
Bebidas , Açúcares , Edulcorantes , Açúcares da Dieta
11.
Front Public Health ; 10: 1046026, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36544796

RESUMO

Objectives: Overweight and obesity are harmful to human health. However, the latest trends of Chinese childhood overweight and obesity prevalence are not available. The aim of this study was to examine the trends from 2000 to 2019 among students in China. Methods: We analyzed data of 66,072 students in the Chinese National Survey on Students' Constitution and Health from 2000 to 2019. Overweight and obesity were defined based on the standard formulated by the International Obesity Task Force (IOTF standard), the World Health Organization (WHO standard), and the Working Group on Obesity in China (WGOC standard), respectively. The χ2-test was used to test the trends of overweight and obesity prevalence and logistic regression was conducted to evaluate the prevalence odds ratios of boys vs. girls and urban vs. rural areas. Results: The prevalence of obesity/overweight and obesity combined was 6.03/23.58% (IOTF standard), 10.56/25.88% (WGOC standard) and 10.75/29.69% (WHO standard) in 2019. From 2000 to 2019, according to the WGOC standard, the prevalence increased from 2.51 to 10.56% for obesity and increased from 9.81 to 25.88% for overweight and obesity combined (P for trend < 0.001). Obesity/overweight and obesity were greater problems in boys than girls and urban than rural areas, but urban-rural differences decreased over time. Conclusion: Overweight and obesity prevalence increased significantly in children and adolescents in China from 2000 to 2019. The prevalence of overweight and obesity in rural areas may contribute to a large percentage of children with overweight and obesity.


Assuntos
Sobrepeso , Obesidade Pediátrica , Masculino , Adolescente , Feminino , Humanos , Criança , Sobrepeso/epidemiologia , Obesidade Pediátrica/epidemiologia , Prevalência , China/epidemiologia , Estudantes
12.
Mikrochim Acta ; 189(12): 460, 2022 11 23.
Artigo em Inglês | MEDLINE | ID: mdl-36416998

RESUMO

With the development of exonuclease, the exonuclease has been used to construct a variety of aptasensor and to realize the signal amplification. Among them, based on silver nanoparticles (Ag NPs) and exonuclease I (Exo I)-assisted cycle signal amplification strategy, we designed a novel high-sensitivity dual-target electrochemical biosensor to detect Pb2+ or Hg2+ in water. In the presence of Hg2+, the Hg2+ was fixed to the aptamer chain by thymine-Hg2+-thymine (T-Hg2+-T), resulting in the decrease of signal. When Pb2+ was present, DNA single strand S2 dissociated and was bound to Pb2+, which automatically triggered Exo I to selectively cut the single chain from the recognition site to achieve the cyclic amplification of the electrochemical signal. The interaction between aptamer and Exo I was investigated by gel electrophoresis. Under the optimum conditions in the scan range -0.20 to 0.60 V, the biosensor had high sensitivity with a linear range of 100 pg/L to 10.0 mg/L, Pb2+ or Hg2+, and the detection limits were 17.0 pg/L (R2 = 0.993) and 12.0 pg/L (R2 = 0.993), respectively. The relative standard deviation (RSD) of the sensor was 0.5-2.6%, and the recovery of spiked standard solutions was between 98.3 and 110%. The cycle amplification strategy supported by this enzyme has promising applications in detection of the two metal ions in various fields.


Assuntos
Mercúrio , Nanopartículas Metálicas , Chumbo , Timina , Prata , Mercúrio/análise , DNA
13.
RSC Adv ; 12(43): 27781-27792, 2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36320242

RESUMO

Qijiao Shengbai Capsule (QSC) is a reputable Miao Nationality medicine used for treating leukopenia, but its chemical composition has not yet been elucidated. We herein present a strategy, by integrating multiple data acquisition, computational data annotation and processing methods to visualize and identify the complicated constituents in QSC based on ultra-high-performance liquid chromatography coupled with traveling wave ion mobility quadrupole time-of-flight mass spectrometry (UPLC-TWIMS-QTOF-MS). The multiple data acquisition modes, including data-independent mass spectrometryEnergy (MSE), data-independent high-definition mass spectrometryEnergy (HDMSE), and fast data-dependent acquisition (fast-DDA), in both positive and negative ion modes, were conducted on a Waters-SYNAPT G2-Si mass spectrometer with an ESI source. An in-house library built by the UNIFI platform could efficiently process the peak annotation of known compounds, whilst different structural types were clustered in the molecular networks for the analogous classification and structural annotation of the unknown ones. Neutral loss, diagnostic ions, feature fragmentation behaviors, and community curation of mass spectrometry data of known compounds helped exploit those similar neighboring nodes of unknown compounds. Moreover, by combination of the predicted CCS values from CCS platform with the experimental CCS values from HDMSE, as well as diagnostic fragment ions, isomer compounds were annotated. By integrating reference compound comparison, a total of 202 constituents, including 94 flavonoids, 12 saponins, 30 phthalides, 38 organic acids, 3 amino acids, 7 alkaloids and 18 others, were unambiguously characterized or tentatively identified in QSC. Among them, 5 potential new compounds were detected and 12 pairs of isomers were comprehensively distinguished. Conclusively, the established multiple acquisition modes, computational data processing and analysis strategy proved to be useful for the in-depth structural identification of QSC.

14.
Food Res Int ; 161: 111842, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36192972

RESUMO

The constantly improved quality of life leads to increasing demand for high-quality food proteins. Thus, the high-efficiency extraction methods of food proteins are highly needed. However, the traditional alkali-solution and acid-isolation method employs a large amount of corrosive acid/alkaline solution, which not only pollutes the environment, but also causes denaturation of proteins. Therefore, developing a novel green extraction method is of great significance. Deep eutectic solvents (DES), characterized by good biodegradability, sustainability and low toxicity, have become a green solvent in the past years. Extraction of food proteins by DES can overcome the shortcomings of corrosive solvents (e.g. NaOH, HCl and H2SO4) used in traditional methods, making it a promising novel green extraction method. In this work, the information on DES was firstly recapped. Furthermore, the latest research progress on the extraction of food proteins by DES was emphatically reviewed. In addition, the challenges and future perspectives for DES-assisted extraction were discussed. Overall, the DES-based methods exhibit high extraction efficiency in extracting animal and plant proteins, amino acids, and enzymes, while the extracted proteins were non-denatured. However, DES-based protein extraction method still faces some challenges, such as high viscosity, poor extraction selectivity and inefficient back-extraction method. The present work can provide a theoretical reference for applying DES in the green extraction of food proteins.


Assuntos
Cáusticos , Solventes Eutéticos Profundos , Aminoácidos , Animais , Proteínas de Plantas , Qualidade de Vida , Hidróxido de Sódio , Solventes/química
15.
Perfusion ; : 2676591221135165, 2022 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-36282873

RESUMO

PURPOSE: To explore the life experiences of patients who have been discharged after undergoing extracorporeal membrane oxygenation (ECMO) support. DESIGN: A qualitative descriptive approach was used. METHODS: Patients who have undergone ECMO support and have been discharged were recruited. Thirteen participants were involved in this study. The data were collected through a semi-structured interview and analyzed using the Colaizzi method. FINDINGS: Four major themes in life experiences were reported by the participants: changes in physical function, changes in psychological state, active adaptation to daily life, and substantial rehabilitation needs. CONCLUSION: Different, continuous, and convenient post-discharge physical and mental interventions, social support, spiritual support, and rehabilitation services should be provided according to the patient's circumstances. We also call on the government to increase the patient reimbursement rate for ECMO treatment. These measures may help to improve the quality of life of patients.

16.
Crit Rev Food Sci Nutr ; : 1-14, 2022 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-36205056

RESUMO

Matrix-associated mycotoxins that bind with macromolecular components through covalent or non-covalent interactions easily occur in various cereals, cereal-based products, and cereal-based feedstuff. They are "masked" by macro-components, causing the underestimation of total exposure risk of mycotoxins. Most of the current reports focus on the free and modified mycotoxins, while the matrix-associated forms are ignored but still can exert toxic effects after ingestion. In this paper, current researches and future prospects of matrix-associated mycotoxins are reviewed. Especially, a focus is set on the transformation of matrix-associated mycotoxins with their free forms during metabolism and food processing. Enzymes, temperature and pH levels during food processing can induce the interconversion of matrix-associated mycotoxins with free mycotoxins. Furthermore, the analytical methods targeted on matrix-associated mycotoxins are discussed. Due to the lack of efficient methods releasing the mycotoxins from matrix, the standard analytical methods has not developed so far. Also, we further analyzed the challenges of matrix-associated mycotoxins about variety, occurrence, toxicity and transformation, exposure assessment, which contributes to establish preventive measures to control their hazards for consumers. Overall, this overview is significant for perfecting risk assessment, as well as developing effective prevention and control actions to matrix-associated mycotoxins.

17.
Front Immunol ; 13: 1043512, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36304472

RESUMO

CUL7, a gene composed of 26 exons associated with cullin 7 protein, is also an E3 ligase that is closely related to cell senescence, apoptosis, and cell transformation and also plays an important role in human cancer. However, there is no systematic pan-cancer analysis has been performed to explore its role in prognosis and immune prediction. In this study, the expression of CUL7 in colon adenocarcinoma (COAD) was investigated to determine its prognosis value. First, based on the Cancer Genome Atlas (TCGA), Genotypic-Tissue Expression Project(GTEx), Cancer Cell Line Encyclopedias(CCLE), and TISIDB database, the potential role of CUL7 in different tumors was explored. Subsequently, the expression of CUL7 in COAD was explored and verified by Immunohistochemistry (IHC). Furthermore, the mutation frequency of CUL7 in COAD was analyzed, and the prognostic value of CUL7 in COAD was discussed. In addition, the nomogram was constructed, and its prognostic value was verified by follow-up data from Jiangmen Central Hospital. Finally, PPI network analysis explored the potential biological function of CUL7 in COAD. The results show that CUL7 is upregulated in most tumors, which is significantly associated with poor survival. At the same time, CUL7 is correlated with the clinical stage and immune landscape of various tumors. In colorectal cancer, CUL7 was overexpressed in tumor tissues by IHC with a mutation frequency of about 4%. CUL7 is an independent prognostic factor for colorectal cancer. The nomogram constructed has effective predictive performance, and external databases proved the prognostic value of CUL7. In addition, PPI network analysis showed that CUL7 was closely related to FBXW8, and further pathway enrichment analysis showed that CUL7 was mainly involved in ubiquitin-mediated proteolysis. Therefore, our study provides a comprehensive understanding of the potential role of CUL7 in different tumors, and CUL7 might be a prognostic marker for COAD.


Assuntos
Adenocarcinoma , Neoplasias do Colo , Humanos , Prognóstico , Neoplasias do Colo/genética , Neoplasias do Colo/patologia , Adenocarcinoma/genética , Adenocarcinoma/patologia , Proteínas Culina/genética , Proteínas Culina/metabolismo , Nomogramas
18.
Biomed Res Int ; 2022: 1913900, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36193301

RESUMO

Objective: To review the features and treatment of parathyroid cancer in our series. Explore the suitable extent of initial surgery and the effect of adjuvant radiotherapy in local recurrence. Methods: Seven cases of parathyroid cancer presented from 2014 to 2021. The presenting features, diagnosis, and treatment are presented. Results: Only two patients had multiple manifestations of hypercalcemia. Marked hypercalcemia, which was revealed to be an average of 13.9 mg/dl (range from 11.8 mg/dl to 15.8 mg/dl), was observed in four patients (57%). The others' serum calcium levels were in the normal range with an average of 9.9 mg/dl (range from 8.6 mg/dl to 10.8 mg/dl). All seven patients had hyperparathyroidism with an average of 733 pg/ml (range from 113 pg/ml to 3193 pg/ml). En bloc resection was performed in two patients with neighboring structure invasion, and four patients with complete tumor capsules underwent tumor resection with limited resection of the thyroid gland. Postoperative adjuvant radiotherapy appeared unsuccessful for local recurrence. Conclusion: High calcium, high PTH, parathyroid occupation by ultrasound, and intraoperative invasion should be considered to have the possibility of parathyroid cancer. Open surgery is recommended and protecting tumor integration is the elementary surgery principle. The initial surgical extent should be decided by the invasion of the tumor. When PC has a local recurrence, the debulking surgery and adjuvant radiotherapy are always fake.


Assuntos
Hipercalcemia , Neoplasias das Paratireoides , Cálcio , Cápsulas , Humanos , Hormônio Paratireóideo , Neoplasias das Paratireoides/diagnóstico , Neoplasias das Paratireoides/cirurgia , Estudos Retrospectivos
19.
J Med Virol ; 2022 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-36258299

RESUMO

With the continuation of the coronavirus disease 2019 pandemic and the emergence of new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants, the control of the spread of the virus remains urgent. Various animals, including cats, ferrets, hamsters, nonhuman primates, minks, tree shrews, fruit bats, and rabbits, are susceptible to SARS-CoV-2 infection naturally or experimentally. Therefore, to avoid animals from becoming mixing vessels of the virus, vaccination of animals should be considered. In the present study, we report the establishment of an efficient and stable system using Newcastle disease virus (NDV) as a vector to express SARS-CoV-2 spike protein/subunit for the rapid generation of vaccines against SARS-CoV-2 in animals. Our data showed that the S and S1 protein was sufficiently expressed in rNDV-S and rNDV-S1-infected cells, respectively. The S protein was incorporated into and displayed on the surface of rNDV-S viral particles. Intramuscular immunization with rNDV-S was found to induce the highest level of binding and neutralizing antibodies, as well as strong S-specific T-cell response in mice. Intranasal immunization with rNDV-S1 provoked a robust T-cell response but barely any detectable antibodies. Overall, the NDV-vectored vaccine candidates were able to induce profound humoral and cellular immunity, which will provide a good system for developing vaccines targeting both T-cell and antibody responses.

20.
Front Cell Infect Microbiol ; 12: 950983, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36093201

RESUMO

Current studies have shown that gut microbiota may be closely related to the severity of coronavirus disease 2019 (COVID-19) by regulating the host immune response. Qing-Fei-Pai-Du decoction (QFPDD) is the recommended drug for clinical treatment of patients with COVID-19 in China, but whether it exerts a therapeutic effect by modulating the immune response through gut microbiota remains unclear. In this study, we evaluated the therapeutic effects of QFPDD in pneumonia model mice and performed 16S rRNA sequencing and serum and lung tissue metabolomic analysis to explore the underlying mechanisms during the treatment. Then, Spearman correlation analysis was performed on gut microbiome, serum metabolome, and immune-inflammation-related indicators. Our results suggest that QFPDD can restore the richness and diversity of gut microbiota, and multiple gut microbiota (including Alistipes, Odoribacter, Staphylococcus, Lachnospiraceae_NK4A136_group Enterorhabdus, and unclassified_f_Lachnospiraceae) are significantly associated with immune-inflammation-related indicators. In addition, various types of lipid metabolism changes were observed in serum and lung tissue metabolome, especially glycerophospholipids and fatty acids. A total of 27 differential metabolites (DMs) were significantly correlated with immune-inflammation-related indicators, including 9 glycerophospholipids, 7 fatty acids, 3 linoleic acid, 2 eicosanoids, 2 amino acids, 2 bile acids, and 2 others. Interestingly, these DMs showed a good correlation with the gut microbiota affected by QFPDD. The above results suggest that QFPDD can improve the immune function and reduce inflammation in pneumonia model mice by remodeling gut microbiota and host metabolism.


Assuntos
Microbiota , Animais , Ácidos Graxos , Glicerofosfolipídeos , Inflamação , Metaboloma , Camundongos , RNA Ribossômico 16S/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...