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2.
Biosci Trends ; 2020 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-32389939

RESUMO

Our purpose was to explore the status of laparoscopic radical resection of hilar cholangiocarcinoma (LRRHcca) in Mainland China. Studies published before February 2020 were retrieved from CNKI database, Pubmed database and Wanfang database. Search terms included "hilar cholangiocarcinoma", "Klatskin tumor", "laparoscopy", "radical operation". Relevant articles regarding LRRHcca in Mainland China were also retrieved. 13 articles were included in this study, with a total of 189 cases. The operation time was 354 min (weighed average, WA), and the mean intraoperative blood loss was 324 mL (WA). The rate of negative margin (R0 rate) was 95.2%, and the number of lymph nodes received was 9.5 (WA). 2.6% of cases were converted to laparotomy. The incidence of postoperative complications was 21.2%, with 3.2% for those classified as Clavien-Dindo ≥ 3, 12.2% for bile leakage, 1.6% for postoperative abdominal hemorrhage, 1.6% for liver insufficiency, and 1.1% for abdominal infection. In-hospital mortality was 0.5%, with mean postoperative hospital stay of 15 days (WA), and the rate of reoperation was 1.1%. The mean postoperative follow-up time was 16 months (WA), and 1-year overall survival rate was 84.5%. In conclusions, laparoscopic radical hilar cholangiocarcinoma is safe and feasible in experienced hands after careful selection of HCCA cases.

3.
Ophthalmol Retina ; 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32389889

RESUMO

PURPOSE: Hyperreflective foci (HRF) are optical coherence tomography (OCT) biomarkers for progression of non-neovascular age-related macular degeneration (AMD) attributed to anteriorly migrated retinal pigment epithelial (RPE) cells. We examined associations between rod- and cone-mediated vision and HRF plus smaller hyperreflective specks (HRS); we sought a histologic candidate for HRS. DESIGN: cross-sectional study; histologic survey PARTICIPANTS: Patients with normal maculas (n=34), early AMD (N=26), and intermediate AMD (N=41) METHODS: AMD severity was determined via the 9-step Age-Related Eye Disease Study scale. In OCT scans HRF and HRS were manually counted. Vision tests probed cones (best corrected visual acuity (VA), contrast sensitivity), mixed cones and rods (low luminance VA, low luminance deficit, mesopic light sensitivity), or rods (scotopic light sensitivity, rod-mediated dark adaptation (RMDA)). An online AMD histopathology resource was reviewed. MAIN OUTCOME MEASURES: Vision in eyes assessed for HRF and HRS; candidate histology for HRS. RESULTS: In 101 eyes of 101 patients, HRF and HRS were identified in 25 and 95 eyes, respectively, with good intra- and inter-rater reliability. HRF were present but sparse in normal eyes, infrequent in early AMD eyes, and frequent but highly variable among intermediate AMD eyes (number per eye, 0.1±0.2, 0.2±0.5, 1.9 ± 3.4; normal, early, intermediate, respectively). HRS outnumbered HRF in all groups (4.5 ± 3.2; 6.3±5.8; 19.4 ± 22.4). Delayed RMDA was strongly associated with more HRF and HRS (both p<0.0001). HRF were also associated with worse low luminance VA (p=0.0117). HRS were associated with worse contrast sensitivity (p=0.0278), low luminance VA (p=0.0010), low luminance deficit (p=0.0031), and mesopic (p=0.0018) and scotopic sensitivity (p<0.0001). By histology, cone lipofuscin was found in inner segments, and the outer nuclear and Henle fiber layers of 25% of normal aged eyes. CONCLUSIONS: HRF and HRS are markers of cellular activity associated with visual dysfunction, especially delayed RMDA, an AMD risk indicator assessing efficiency of retinoid re-supply. HRS may represent lipofuscin granules translocating inwardly within cone photoreceptors. Visible and quantifiable on SD-OCT, HRF and HRS may serve as structural endpoints in clinical trials targeting AMD stages earlier than atrophy expansion. These results should be confirmed in a larger sample.

4.
Sci Rep ; 10(1): 7973, 2020 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32409649

RESUMO

Aflatoxin B1 (AFB1) contamination in foods is an important health challenge for low-and middle-income countries in subtropical regions. AFB1 has been detected in a variety of foodsin Guangzhou, while the risk of dietary exposure is unknown. This study aimed to assess the probabilistic risk of dietary exposure to AFB1 contamination in food stuffs in Guangzhou by using margin of exposure (MOE) and quantitative liver cancer risk approaches. A total of1854 AFB1-contaminated foodstuffs were sampled in supermarkets, agricultural markets, retail shops, and family workshops from 11 districts of Guangzhou, and AFB1 content was determined by HPLC-fluorescence detector. In total, 9.9% (184/1854) of the test samples had AFB1 concentrations above the limit of detection. Home-made peanut oil had the highest AFB1 concentration, with a mean value of 38.74 ± 47.45 µg kg-1. The average MOE levels of Guangzhou residents ranged from 100 to 1000. The risk of liver cancer was 0.0264 cancers (100,000 population year)-1. The health risks of suburban people were higher than those of urban people, and home-made peanut oil was the main contributorto dietary exposure to AFB1 among suburban residents in Guangzhou. The production of home-made peanut oil should be supervised to reduce the risk of AFB1 exposure.

5.
Sci Adv ; 6(15): eaay6825, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32284997

RESUMO

Two-dimensional nanomaterial-based photothermal therapy (PTT) is currently under intensive investigation as a promising approach toward curative cancer treatment. However, high toxicity, moderate efficacy, and low uniformity in shape remain critical unresolved issues that hamper their clinical application. Thus, there is an urgent need for developing versatile nanomaterials to meet clinical expectations. To achieve this goal, we developed a stable, highly uniform in size, and nontoxic nanomaterials made of tellurium-selenium (TeSe)-based lateral heterojunction. Systemic delivery of TeSe nanoparticles in mice showed highly specific accumulation in tumors relative to other healthy tissues. Upon exposure to light, TeSe nanoparticles nearly completely eradicated lung cancer and hepatocellular carcinoma in preclinical models. Consistent with tumor suppression, PTT altered the tumor microenvironment and induced immense cancer cell apoptosis. Together, our findings demonstrate an exciting and promising PTT-based approach for cancer eradication.

6.
Emerg Infect Dis ; 26(3): 516-522, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32091373

RESUMO

The high incidence of tuberculosis (TB) among prisoners calls for interventions to identify latent tuberculosis infection (LTBI) before disease onset. To identify LTBI prevalence among prisoners and factors associated with it, we conducted a cross-sectional study in Tianjin. We randomly sampled 959 HIV-negative adult prisoners by ward clusters in 5 prisons and determined LTBI by seropositivity using an interferon-γ release assay. The overall rate of LTBI was 52.0% (499/959) in the 5 facilities and ranged from 41.9% (72/172) to 60.9% (106/174). Age (adjusted odds ratio [aOR] 1.7, 95% CI 1.4-2.0 per 10 years), duration of imprisonment (aOR 1.2, 95 CI% 1.1-1.2 per year), previous incarceration (aOR 2.0, 95% CI 1.5-2.7), and facility-specific TB incidence (aOR 1.9, 95% CI 1.3-2.8) were risk factors for LTBI. These findings indicate possible TB transmission within prisons and suggest the necessity for early TB case detection, as well as prophylaxis.

7.
Bioresour Technol ; 303: 122868, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32032936

RESUMO

This study is the first to investigate the changes in the composting process and carbon conversion in a cow manure-straw compost matrix with Bacillus subtilis added at four different levels (0, 0.5%, 1%, and 2% w/w compost), and to explain the mechanism responsible for carbon conversion through microbial functional metabolism. Inoculation with Bacillus subtilis at 2% had the best effect on fermentation among all treatments, but it inhibited the synthesis of total organic carbon and humus. Bacillus subtilis at 0.5% reduced mineralization in the cooling and maturity stages of composting, and enhanced the humification of carbon. The total organic carbon and humic sequence contents were significantly higher with Bacillus subtilis at 0.5% (12.5% and 20.2%, respectively) than Bacillus subtilis at 2% (P < 0.05). Redundancy analysis demonstrated that the pH and microbial functional metabolism were closely related to carbon sequestration during composting.


Assuntos
Compostagem , Animais , Bacillus subtilis , Carbono , Bovinos , Feminino , Esterco , Solo
8.
Ophthalmol Retina ; 4(3): 274-283, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31924545

RESUMO

PURPOSE: To describe the progression and regression of individual subretinal drusenoid deposits (SDDs) and surrounding photoreceptors and retina in patients with age-related macular degeneration (AMD) over a 3.5-year period using multimodal imaging including adaptive optics scanning laser ophthalmoscopy (AOSLO). DESIGN: Longitudinal observational study. PARTICIPANTS: Four patients with intermediate AMD. METHODS: Six eyes of 4 patients with intermediate AMD each were imaged 4 times over 3.5 years. Five eyes of 3 patients showed only SDD and no drusen. Subretinal drusenoid deposit presence and progression were assessed by multimodal imaging and a 3-stage grading system based on spectral-domain (SD) OCT. Morphologic features and the fine structure of individual SDD lesions identified at baseline were examined by AOSLO at follow-up visits. Reflectivity of photoreceptors surrounding SDD were assessed with AOSLO and SD OCT. MAIN OUTCOME MEASURES: Morphologic features, fine structure, and size of individual SDD lesions by AOSLO; photoreceptor integrity surrounding SDD via AOSLO and SD OCT; and retinal layer thicknesses via SD OCT. RESULTS: Individual SDDs followed independent lifecycle trajectories, exhibiting growth, shrinkage, fusion, and disappearance. Alterations in shape, morphologic features, and internal structure were not obviously the result of the presence of invading phagocytes. Of 822 lesions across all stages examined at baseline, 566 (69%) grew, 123 (15%) shrank, 47 (6%) remained of similar size, 86 (11%) disappeared, and 5 (0.6%) reappeared after regression. A return of characteristic photoreceptor reflectivity in AOSLO (punctate) and in SD OCT (prominent ellipsoid zone) was observed after regression of some SDD in 5 eyes of 4 patients. All eyes exhibited thinning of photoreceptor layers, despite intact retinal pigment epithelium (RPE), to approximately 70% of baseline thicknesses, as well as poorly visible or undetectable outer retinal bands. CONCLUSIONS: Adaptive optics scanning laser ophthalmoscopy and SD OCT imaging of individual SDDs over 3.5 years revealed independent trajectories of progression and regression, believed to reflect the activities of local outer retinal cells. Restoration of some photoreceptor reflectivity and intact RPE after SDD regression should be seen in the larger context of outer retinal atrophy, previously suggested as a new form of advanced AMD, and herein replicated.

9.
J Cell Physiol ; 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31951023

RESUMO

Tripartite motif containing 59 (TRIM59) functions as an oncoprotein in various human cancers including ovarian cancer. In this study, we found that TRIM59 gene amplification was prevalent in ovarian cancer tissues, and its amplification was significantly correlated with poorer overall survival. Moreover, knockdown of TRIM59 in SKOV3 and OVCAR3 cells, which had relatively high level of TRIM59, suppressed glucose uptake and lactate production. TRIM59 knockdown also decreased the expression of c-Myc and lactate dehydrogenase A, and the phosphorylation of extracellular signal-regulated kinase (ERK). TRIM59 overexpression in A2780 cells, which expressed low level of TRIM59, showed reverse effects. Notably, treatment with an ERK inhibitor (PD98059) completely abolished the oncogenic effects of TRIM59 overexpression. Interestingly, TRIM59 increased the ubiquitination of MAP kinase phosphatase 3 (MKP3), which may dephosphorylate and inactivate ERK. Ectopic expression of MKP3 inhibited the promoting effects of TRIM59 on glycolysis and the phosphorylation of ERK. TRIM59 protein expression was negatively correlated with MKP3 protein expression in ovarian cancer tissues. Finally, TRIM59 amplification potently affected the anticancer effect of 3-bromopyruvate, an inhibitor of glycolysis, in ovarian cancer cells and patient-derived xenograft. In conclusion, these results suggest that TRIM59 may regulate glycolysis in ovarian cancer via the MKP3/ERK pathway.

10.
Res Vet Sci ; 128: 153-161, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31809972

RESUMO

Hen eggs (HEs) provide valuable nutrients for humans, including proteins, carbohydrates, lipids and vitamins. Recent studies revealed a number of novel egg-derived proteins/peptides (EDPs), and EDPs may play a crucial role in food industry and medical therapy. First, these EDPs were purified from the enzyme-catalyzed hydrolysates of egg proteins and were characterized by biochemical assays such as gel electrophoresis, HPLC, mass spectrometry, proteomic and peptideomic analysis, etc. Second, some EDPs can be used as nontoxic bio-preservatives and functional nutraceuticals for replacing harmful sodium nitrite, inhibiting foodborne pathogens, promoting metal-ion absorption and improving meat-product quality, and these new features will be widely used in the field of food production. Third, novel medical properties of EDPs comprise anti-oxidative, anti-microbial, anti-inflammatory and anti-nociceptive activities, which will benefit prevention of cardiovascular diseases, cancers, diabetic mellitus, immune disorders, etc. In summary, this review gives a real insight into the novel nutritional, biological and medical functions of EDPs, predictably facilitating the applications of EDPs in production of nutritive supplements, functional nutraceuticals and therapeutic medicines.

11.
J Nanosci Nanotechnol ; 20(2): 1232-1237, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383123

RESUMO

CO atmosphere plasma and first H2 then CO plasma were attempted to substitute the traditional "calcination-reduction-carburization" processes for the preparation of metallic cobalt phase with hcp structure. CoRu/SiO2 catalyst precursor was prepared via incipient wetness impregnation. Characterization and catalytic results showed that CO could be decomposed in glow discharge plasma field, a large fraction of carbon species was deposited as elementary substance on the catalyst surface, while some of the carbon reacted with cobalt and formed cobalt carbide, which could transform into Co0 with hcp structure after reduction. The hcp structured cobalt containing catalysts showed higher initial Fischer-Tropsch synthesis activity than both calcined and air plasma treated samples.

12.
Biosci Trends ; 13(6): 488-501, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31875583

RESUMO

The development of Minimally invasive pancreaticoduodenectomy (MIPD) in Chinese mainland has been extremely quick. However, the safety and oncologic outcomes remain controversial. This review evaluates the current status of MIPD in Chinese mainland. A systematic literature search was performed using: Pubmed, Web of Sci, CNKI, Wanfang Data and Sinomed databases to filter all studies published up to and including June 2019 using key words "pancreaticoduodenectomy," or "Whipple operation" combined with "laparoscopy," or "laparoscopic," or "robotic," or "da Vinci," or "minimally invasive," or "hand-assisted". This systematic review included 39 articles that documented 2,653 MIPDs in Chinese mainland. The weighted average operative time was 370.6 min, and the weighted average blood loss was 278.0 mL. The overall morbidity was 31.9%, which Clavien-Dindo ≥ 3 complications accounted for 13.4%. Pancreatic fistula, delayed gastric emptying, bile leak and postoperative hemorrhage were reported in 20.9%, 5.5%, 3.5% and 6.0% of patients respectively. The average lengh of hospital stay was 16.1 days. The overall surgical mortality was 1.7%. The mean number of harvested lymph nodes was 13.5, and the rate of positive margin was 5.3%. Based on Chinese national condition, the operative volume of MIPD in Chinese mainland is the leading position in the world, and compared with some large international meta-analysis, no inferior perioperative and short-term oncological outcomes were observed in MIPD of Chinese mainland. However, research on survival analysis and phased learning curve outcomes is needed urgently before the innovative techniques are widely accepted.

13.
Pediatr Infect Dis J ; 39(1): 70-77, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31725555

RESUMO

BACKGROUND: Evaluation of a pneumococcal conjugate vaccine (PCV) in an animal model provides an initial assessment of the performance of the vaccine prior to evaluation in humans. Cost, availability, study duration, cross-reactivity and applicability to humans are several factors which contribute to animal model selection. PCV15 is an investigational 15-valent PCV which includes capsular polysaccharides from pneumococcal serotypes (ST) 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 22F, 23F and 33F all individually conjugated to cross-reactive material 197 (CRM197). METHODS: Immunogenicity of PCV15 was evaluated in infant rhesus macaques (IRM), adult New Zealand white rabbits (NZWR) and CD1 mice using multiplexed pneumococcal electrochemiluminescent (Pn ECL) assay to measure serotype-specific IgG antibodies, multiplexed opsonophagocytosis assay (MOPA) to measure serotype-specific functional antibody responses and bacterial challenge in mice to evaluate protection against a lethal dose of S. pneumoniae. RESULTS: PCV15 was immunogenic and induced both IgG and functional antibodies to all 15 vaccine serotypes in all animal species evaluated. PCV15 also protected mice from S. pneumoniae serotype 14 intraperitoneal challenge. Opsonophagocytosis assay (OPA) titers measured from sera of human infants vaccinated with PCV15 in a Phase 2 clinical trial showed a good correlation with that observed in IRM (rs=0.69, P=0.006), a medium correlation with that of rabbits (rs=0.49, P=0.06), and no correlation with that of mice (rs=0.04, P=0.89). In contrast, there was no correlation in serum IgG levels between human infants and animal models. CONCLUSIONS: These results demonstrate that PCV15 is immunogenic across multiple animal species, with IRM and human infants showing the best correlation for OPA responses.

14.
J Control Release ; 316: 208-222, 2019 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-31682909

RESUMO

The clinical treatment of melanoma continues to present many challenges including poor prognosis because neither monotherapy nor combination therapies have shown maximal treatment efficacy. In this study, an enzyme-responsive nanoparticle was designed for tumor subtypes with the high expression of heparanase-1, since highly metastatic tumors such as melanoma generally express significant levels of heparanase-1. PTX-DOTAP@alloferon-1-heparin/protamine, an enzyme-responsive nanoparticle, has a particle size of 106.1 ± 1.113 nm and a ζ-potential of -45.1 ± 0.455 mV, which enables enrichment in the tumor site by passive targeting. Subsequently, heparanase-1, which is highly expressed in the extracellular matrix, rapidly recognizes and degrades heparin in the outer layer of the nanoparticle and releases encapsulated alloferon-1 by ion diffusion to activate inhibited NK cells in the tumor microenvironment. The size of the smart nanoparticle will eventually decrease to 59.30 ± 0.783 nm and the ζ-potential will reverse to 25.4 ± 0.257 mV, which is beneficial for deep penetration and tumor cell uptake (due to the high negative charge on the tumor cell surface) of PTX-DOTAP cores. Paclitaxel is released in the cytoplasm, and the tumor cells are arrested in the G2/M phase. The nanoparticle characterization experiment demonstrated that in vivo drug delivery could be completed. In subsequent cell and animal experiments, the experimental data demonstrated the efficient therapeutic effects of the nanoparticle. This study provides an excellent template nanoparticle for the treatment of highly metastatic tumors to enhance future prognosis.

15.
Retina ; 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31599795

RESUMO

PURPOSE: To clarify the role of subretinal drusenoid deposits (SDD; pseudodrusen) in the progression of age-related macular degeneration through high-resolution histology. METHODS: In 33 eyes of 32 donors (early age-related macular degeneration, n = 15; geographic atrophy, n = 9; neovascular age-related macular degeneration, n = 7; unremarkable, n = 2), and 2 eyes of 2 donors with in vivo multimodal imaging including optical coherence tomography, examples of SDD contacting photoreceptors were assessed. RESULTS: Subretinal drusenoid deposits were granular extracellular deposits at the apical retinal pigment epithelium (RPE); the smallest were 4-µm wide. Outer segment (OS) fragments and RPE organelles appeared in some larger deposits. A continuum of photoreceptor degeneration included OS disruption, intrusion into inner segments, and disturbance of neurosensory retina. In a transition to outer retinal atrophy, SDD appeared to shrink, OS disappeared, inner segment shortened, and the outer nuclear layer thinned and became gliotic. Stage 1 SDD on optical coherence tomography correlated with displaced OS. Confluent and disintegrating Stage 2 to 3 SDD on optical coherence tomography and dot pseudodrusen by color fundus photography correlated with confluent deposits and ectopic RPE. CONCLUSION: Subretinal drusenoid deposits may start at the RPE as granular, extracellular deposits. Photoreceptor OS, RPE organelles, and cell bodies may appear in some advanced deposits. A progression to atrophy associated with deposit diminution was confirmed. Findings support a biogenesis hypothesis of outer retinal lipid cycling.

16.
Anal Chim Acta ; 1089: 131-143, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31627810

RESUMO

In this work, the as-prepared V2O5 nanobelts can sensitively quench the fluorescence of nitrogen-doped carbon dots (N-CDs) based on the inner filter effect (IFE). In the presence of ascorbic acid (AA), the fluorescence of N-CDs can recover through the redox reaction between V2O5 nanobelts and AA. Meanwhile, in the presence of both alkaline phosphatase (ALP) and ascorbyl-2-phosphate (AAP), the fluorescence of N-CDs can also restore since AAP can be hydrolyzed into AA by ALP. Under optimum conditions, the linear range for AA is from 0.01 to 2.5 µM with a detection limit of 3 nM and that for ALP is from 0.1 to 30 U/L with a detection limit of 0.04 U/L (S/N = 3). Particularly, the proposed probe could be successfully used to detect AA and ALP in human serum samples. Furthermore, N-CDs can be applied in fluorescence imaging of Human breast cancer cells with satisfactory results.


Assuntos
Fosfatase Alcalina/sangue , Ácido Ascórbico/sangue , Corantes Fluorescentes/química , Pontos Quânticos/química , Compostos de Vanádio/química , Técnicas Biossensoriais/métodos , Carbono/química , Carbono/toxicidade , Linhagem Celular Tumoral , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/toxicidade , Humanos , Limite de Detecção , Microscopia de Fluorescência/métodos , Nitrogênio/química , Nitrogênio/toxicidade , Pontos Quânticos/toxicidade , Espectrometria de Fluorescência/métodos , Compostos de Vanádio/toxicidade
17.
Biosci Trends ; 13(5): 456-460, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31611521

RESUMO

Intraductal papillary mucinous neoplasm (IPMN) of the pancreas is no longer a rarely diagnosed disease, because of the development of medical imaging. With a high incidence of canceration, especially in the main duct type, surgery is strongly recommended. Pancreatoduodenectomy, distal pancreatectomy and central pancreatectomy are applied in those cases. For this potentially malignant disease, function-preserving surgery seems more appropriate. An old female was enrolled in our research, who was diagnosed with IPMN. Diameter of the main pancreatic duct (MPD) was > 5 mm and lesions distributed to the whole pancreas. laparoscopic duodenum and spleen-preserving total pancreatoduodenectomy was carried out, which has not reported previously. We successfully performed laparoscopic duodenum and spleen-preserving total pancreatectomy, without major complications such as severe pancreatic fistula, postoperative bleeding, and delayed ischemia of duodenum and spleen. We consider laparoscopic duodenum and spleen-preserving total pancreatectomy is technically feasible, but a large sample of randomized controlled trials is needed to evaluate its safety, effectiveness and long-term outcome.

18.
Surg Endosc ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31664578

RESUMO

BACKGROUND: Radical resection is the only curative option for patients with hilar cholangiocarcinoma (HCCA) to achieve long-term survival. However, due to the fact that radical resection of HCCA has high technical requirements, the safety and efficacy of laparoscopic resection for HCCA remains controversial. METHOD: From January 2015 to December 2018, 23 cases of HCCA underwent radical resection in our center. Clinical data of those patients were collected and analyzed retrospectively. RESULTS: 14 patients underwent laparoscopic resection and 9 cases received open resection. 2 patients in laparoscopic group were converted to laparotomy. Operation time in laparoscopic group was significantly longer than that in open group (519.4 ± 155.4 min vs 366.7 ± 93.1 min). Estimated blood loss (620.0 ± 681.2 ml vs 821.4 ± 713.8 ml) and incidence of intraoperative blood transfusion (5/9 vs 8/14) did not differ significantly between two groups. Pathological outcomes were comparable between two groups. Length of postoperative hospital stay (23.4 ± 13.4 days vs 17.8 ± 7.1 days), severe postoperative morbidity (3/9 vs 5/14), bile leakage of Grade A or B (5/9 vs 5/14), intra-abdominal bleeding (0/9 vs 1/14), intra-abdominal abscess (1/9 vs 0/14), wound infection (0/9 vs 1/14), pulmonary infection (2/9 vs 0/14), and liver failure (0/9 vs 0/14) did not differ significantly between two groups. One patient in laparoscopic group died (1/14) at 21 postoperative days due to intra-abdominal bleeding, while no 30-day mortality was observed in open group. CONCLUSION: Our results demonstrate that laparoscopic radical resection of HCCA is safe and feasible in experienced hands. Although laparoscopic resection for HCCA, which is still in initial and exploratory stage, fails to show any advantage over open resection in this study, we are optimistic with its wide application in future with the improvement of surgical techniques and experience.

19.
Anal Bioanal Chem ; 411(27): 7137-7146, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31511944

RESUMO

The application of carbon dots as a coreactant for Ru(bpy)32+ (where bpy is 2,2'-bipyridine) electrochemiluminescence (ECL) has been widely studied. However, the high cost of Ru(bpy)32+ and its derivatives has prohibited its widespread use in ECL biosensors. Herein, a novel anodic ECL system based on sulfur-doped graphitic carbon nitride nanosheets (S-g-C3N4 NSs) and nitrogen-doped carbon dots (N-CDs) is presented. In this ECL system, N-CDs serve as a new ECL coreactant that can significantly enhance the anodic ECL signal of S-g-C3N4 NSs (approximately 83 times) under optimal conditions. The possible ECL response mechanism of the S-g-C3N4 NSs/N-CDs system is proposed in detail on the basis of cyclic voltammograms, ECL-time curves, and ECL spectra. Furthermore, the ECL signal of the S-g-C3N4 NSs/N-CDs system was quenched by folic acid (FA), which was chosen as a model analyte to study the potential application of the new ECL system. The ECL intensity decreased linearly with the concentration of FA in the range from 0.05 to 200 µM. The detection limit for FA measurement is 16 nM (signal-to-noise ratio of 3). The proposed new ECL system has many advantages over traditional approaches, such as low toxicity and excellent biocompatibility. Especially, the proposed approach can detect FA in diluted human serum samples with satisfactory recoveries, indicating promising application for bioanalysis. Graphical abstract.


Assuntos
Carbono/química , Técnicas Eletroquímicas/métodos , Eletrodos , Ácido Fólico/sangue , Nanoestruturas/química , Nitrilos/química , Nitrogênio/química , Enxofre/química , Técnicas Biossensoriais , Técnicas Eletroquímicas/instrumentação , Humanos , Limite de Detecção , Luminescência , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
20.
Chem Commun (Camb) ; 55(71): 10559-10562, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31417988

RESUMO

Pure-phase cobalt nanocrystals with well-defined specific exposed facets were synthesized via controllable reduction of their CoO counterparts while retaining the same scale of particle size. Three different catalysts, i.e. hexagonal close-packed (hcp) Co pyramid with (10-11) and rodlike with (10-10) facets, as well as face-centred cubic (fcc) Co octahedron with (111) exposed were obtained and studied for Fischer-Tropsch synthesis (FTS) reaction. No noticeable changes of either the shape or the exposed facets were found under practical FTS reaction conditions. The hcp (10-11) facet exhibits the highest FTS activity and C5+ product selectivity with the lowest apparent activation energy and CH4 selectivity. Theoretical calculations of the energy barrier for CO dissociation and methanation of the reaction intermediate CHx rationalize the experimental results.

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