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1.
Chin J Physiol ; 64(2): 80-87, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33938818

RESUMO

Ca2+-sensing receptors (CaSR), activated by elevated concentrations of extracellular Ca2+, have been known to regulate functions of thyroid cells, neurons, and endothelial cells (EC). In this report, we studied CaSR-mediated Ca2+ influx in mouse cerebral microvascular EC (bEND.3 cells). Cytosolic free Ca2+ concentration and Mn2+ influx were measured by fura-2 microfluorometry. High (3 mM) Ca2+ (CaSR agonist), 3 mM spermine (CaSR agonist), and 10 µM cinacalcet (positive allosteric modulator of CaSR) all triggered Ca2+ influx; however, spermine, unlike high Ca2+ and cinacalcet, did not promote Mn2+ influx and its response was poorly sensitive to SKF 96365, a TRP channel blocker. Consistently, 2-aminoethoxydiphenyl borate and ruthenium red (two other general TRP channel blockers) suppressed Ca2+ influx triggered by cinacalcet and high Ca2+ but not by spermine. Ca2+ influx triggered by high Ca2+, spermine, and cinacalcet was similarly suppressed by A784168, a potent and selective TRPV1 antagonist. Our results suggest that CaSR activation triggered Ca2+ influx via TRPV1 channels; intriguingly, pharmacological, and permeability properties of such Ca2+ influx depended on the stimulating ligands.

2.
J Agric Food Chem ; 69(15): 4423-4437, 2021 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-33835816

RESUMO

Anthocyanins have been reported to possess antidiabetic effects. Recent studies indicate acylated anthocyanins have better stability and antioxidative activity compared to their nonacylated counterparts. This study compared the effects of nonacylated and acylated anthocyanins on hepatic gene expression and metabolic profile in diabetic rats, using full-length transcriptomics and 1H NMR metabolomics. Zucker diabetic fatty (ZDF) rats were fed with nonacylated anthocyanin extract from bilberries (NAAB) or acylated anthocyanin extract from purple potatoes (AAPP) at daily doses of 25 and 50 mg/kg body weight for 8 weeks. Both anthocyanin extracts restored the levels of multiple metabolites (glucose, lactate, alanine, and pyruvate) and expression of genes (G6pac, Pck1, Pklr, and Gck) involved in glycolysis and gluconeogenesis. AAPP decreased the hepatic glutamine level. NAAB regulated the expression of Mgat4a, Gstm6, and Lpl, whereas AAPP modified the expression of Mgat4a, Jun, Fos, and Egr1. This study indicated different effects of AAPP and NAAB on the hepatic transcriptomic and metabolic profiles of diabetic rats.

3.
Nutrients ; 13(5)2021 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-33925556

RESUMO

Our knowledge related to human milk proteins is still limited. The present study determined the changes in multiple human milk proteins during the first six months of lactation, investigated the influencing factors of milk proteins, and explored the impact of milk proteins on infant growth. A total of 105 lactating women and their full-term infants from China were prospectively surveyed in this research. Milk samples were collected at 1-5 days, 8-14 days, 1 month, and 6 months postpartum. Concentrations of total protein and α-lactalbumin were measured in all milk samples, and concentrations of lactoferrin, osteopontin, total casein, ß-casein, αs-1 casein, and κ-casein were measured in milk from 51 individuals using ultra performance liquid chromatography coupled with mass spectrometry. The concentration of measured proteins in the milk decreased during the first six months of postpartum (p-trend < 0.001). Maternal age, mode of delivery, maternal education, and income impacted the longitudinal changes in milk proteins (p-interaction < 0.05). Concentrations of αs-1 casein in milk were inversely associated with the weight-for-age Z-scores of the infants (1 m: r -0.29, p 0.038; 6 m: r -0.33, p 0.020). In conclusion, the concentration of proteins in milk decreased over the first six months postpartum, potentially influenced by maternal demographic and delivery factors. Milk protein composition may influence infant weights.

4.
Exp Physiol ; 2021 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-33866642

RESUMO

NEW FINDINGS: Previous studies have suggested WML is a brain disease characterized by altered brain structural and functional connectivity. However, findings have shown an inconsistent pattern. One of the reasons may be due to the potential subtypes of WML manifestation and related brain structural abnormalities. We examined whether there are distinct cortical thickness changes in patients with WML subtypes. Our results suggested that WML-VCIND and WML-VaD patients showed distinct pathophysiology in cortical thickness. These neural correlates of WML should be considered in future treatment. ABSTRACT: Background : The effect of cortical thickness on white matter lesions (WML) in patients with distinct vascular cognitive impairments is relatively unknown. This study investigated the correlation between cortical thickness and vascular cognitive manifestations. METHODS: WML patients and healthy controls (HCs) from Beijing Tiantan Hospital between 2014 and 2018 were included. The patients were further divided into two subgroups, namely WML with non-dementia vascular cognitive impairment (WML-VCIND) and WML with vascular dementia (WML-VaD) group according to Clinical Dementia Rating scale (CDR) and the Beijing version of Montreal Cognitive Assessment (MoCA). Changes in cortical thickness were calculated using FreeSurfer. Pearson's correlation analysis was conducted to explore the relationship between cognitive manifestations and cortical thickness in WML patients. RESULTS: 45 WML patients and 23 HCs were recruited. The WML group exhibited significant difference in cortical thickness compared to the control group. Significantly decreased cortical thickness in the middle and superior frontal gyri, middle temporal gyrus, angular gyrus, and insula was found in the WML-VaD versus WML-VCIND subgroup. Cortical thickness deficits of the left caudal middle frontal gyrus (r = 0.451, P = 0.002), left rostral middle frontal gyrus (r = 0.514, P < 0.001), left superior frontal gyrus (r = 0.410, P = 0.006), right middle temporal gyrus (r = 0.440, P = 0.003), right pars triangularis (r = 0.462, P = 0.002), right superior frontal gyrus (r = 0.434, P = 0.004) and right insula (r = 0.499, P = 0.001) were positively correlated with the MoCA score in WML patients. CONCLUSION: The specific pattern of cortical thickness deficits in the WML-VaD subgroup revealed the pathophysiology of WML, which should be considered in future treatment of WML. This article is protected by copyright. All rights reserved.

5.
Rev Cardiovasc Med ; 22(1): 239-245, 2021 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-33792268

RESUMO

The burden of cardiovascular disease is predicted to escalate in developing countries. The aim of this study is to assess the characteristics, management strategies and outcomes of the patients with acute coronary syndrome (ACS) who were admitted to hospitals under the chest pain center mode in southwest P. R. China. Adults hospitalized with a diagnosis of ACS were enrolled in the retrospective, observational registry between January 2017 and June 2019 at 11 hospitals in Chengdu, P. R. China. The collected data included the patients' baseline characteristics, clinical management and in-hospital outcomes. After Statistical analysis, (1) A total of 2857 patients with ACS, among which 1482 have ST-segment elevation myocardial infarction (STEMI), 681 have non-STEMI (NSTEMI) and 694 have unstable angina (UA) were enrolled in the study. (2) 61.3% of the ACS patients received reperfusion therapy. More patients with STEMI underwent percutaneous coronary intervention (PCI) compared with NSTEMI/UA patients (80.6% vs. 38.8%, P < 0.001), while thrombolytics were administered in only 1.8% of STEMI patients. (3) The median time from symptoms to hospital was 190 min (IQR 94-468) in STEMI, 283 min (IQR 112-1084) in NSTEMI and 337 min (IQR 97-2220) in UA (P < 0.001), and the door-to-balloon time for primary PCI (pPCI) was 85 min (IQR 55-121) in STEMI. (4) The in-hospital outcomes for STEMI patients included death (8.1%) and acute heart failure (22.6%), while the outcomes for those with NSTEMI and UA were better: death (4.0% and 0.9%, P < 0.001) and acute heart failure (15.3% and 9.9%, P < 0.001). (5) Antiplatelet drugs, lipid-lowering drugs, ß-blockers and angiotensin-converting enzyme inhibitors (ACEI) /angiotensin receptor blockers (ARB) were used in about 98.3%, 95.0%, 67.7% and 54.3% of the ACS patients, respectively. Therefore, the management capacity in Chengdu has relatively increased compared with previous studies, but important gaps still exist compared with developed countries, especially regarding the management of the NSTEMI/UA patients.

6.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 33(3): 318-323, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33834973

RESUMO

OBJECTIVE: To assess the age-related differences in the management strategies and outcomes of patients with acute coronary syndrome (ACS) under the chest pain center model. METHODS: Clinical data of 2 833 patients with ACS were enrolled in the retrospective observational registry between January 2017 and June 2019 at 11 hospitals with chest pain centers in Chengdu. The patients were divided into four groups according to their ages: < 55 years old group (n = 569), 55-64 years old group (n = 556), 65-74 years old group (n = 804), ≥ 75 years old group (n = 904). The collected data included the patients' demographic characteristics, cardiovascular risk factors, medical history, symptoms and signs of onset, experimental examination, types of ACS and the time from the symptom to the hospital (S-to-D), etc., and the clinical characteristics, management strategies, all-cause mortality in the hospital, and the incidence of major adverse cardiovascular and cerebrovascular events (MACCE) within 1 year after discharge were compared. The primary end point was the clinical outcome of ACS patients in different age groups, including all-cause deaths in the hospital and the incidence of MACCE within 1 year after discharge. The secondary end point was the proportion of ACS patients underwent percutaneous coronary intervention (PCI) in different age groups. Multivariate Logistic regression was used to analyze the risk factors of all-cause deaths in ACS patients. Kaplan-Meier curve was used to express the incidence of MACCE within 1 year after discharge in different age groups. Multivariate Cox regression was used to analyze the factors affecting the incidence of MACCE within 1 year after discharge of ACS patients. RESULTS: As age increased, the proportion of male patients gradually decreased, and the percentages of male patients aged < 55 years old, 55-64 years old, 65-74 years old, and ≥ 75 years old were 87.2% (496/569), 77.0% (428/556), 66.4% (534/804), and 60.1% (543/904), respectively; and ACS patients combined with hypertension, diabetes, coronary heart disease, and stroke history were more common [the percentages of patients with hypertension aged < 55 years old, 55-64 years old, 65-74 years old, ≥ 75 years old were 41.3% (235/569), 52.2% (290/556), 59.7% (480/804), and 66.9% (605/904); the percentages of diabetes were 18.6% (106/569), 25.5% (142/556), 27.0% (217/804), and 28.2% (255/904); the percentages of coronary heart disease were 10.1% (57/564), 13.9% (77/555), 17.6% (141/803), and 23.7% (213/899); the percentages of stroke were 0.7% (4/564), 4.0% (22/552), 4.5% (36/801), and 8.6% (77/894)]. But the percentages of patients with a history of active smoking, typical chest pain/chest tightness and dyslipidemia were significantly reduced [the percentages of smoking history were 60.2% (340/565), 48.0% (266/554), 33.7% (270/801), and 21.7% (195/899), typical chest pain/chest tightness were 96.9% (536/553), 96.4% (516/535), 91.8% (716/780), 90.2% (776/860); the percentages of dyslipidemia were 11.2% (63/565), 9.2% (51/553), 5.7% (46/802), and 4.9% (44/896)], the time of S-to-D was significantly prolonged [minutes: 176.0 (73.5, 557.0), 194.5 (89.3, 682.3), 221.0 (98.8, 940.5), and 270.0 (115.0, 867.0)], hemoglobin (Hb) level was significantly reduced (g/L: 145.44±17.43, 135.95±19.25, 129.75±19.03, 122.19±20.55), and the incidence of non-ST-segment elevation myocardial infarction (NSTEMI) increased significantly [18.6% (106/569), 20.5% (114/556), 26.6% (214/804), 26.5% (240/904)], and the differences were statistically significant (all P < 0.05). The proportion of Killip grade III-IV were the highest in patients aged ≥ 75 years old, 9.0% and 12.6%, respectively. Compared with the groups aged < 55 years old, 55-64 years old, and 65-74 years old, the proportion of patients aged ≥ 75 years old who underwent PCI was the lowest, and the all-cause mortality in the hospital and the incidence of 1-year MACCE of patients underwent PCI were significantly lower than those of patients underwent conservative treatment [6.0% (28/463) vs. 10.4% (45/434), 14.6% (43/294) vs. 24.3 % (55/226), both P < 0.05]. As age increased, the hospital all-cause mortality and the 1-year MACCE incidence increased (all-cause mortality rates in < 55 years old, 55-64 years old, 65-74 years old, ≥ 75 years old groups were 0.9%, 2.2%, 5.5%, 8.3%, and the 1-year MACCE incidences were 5.0%, 6.7%, 13.9%, 18.7%, both P < 0.01). The multivariate Logistic regression analysis showed that age, cardiogenic shock, ST-segment elevation myocardial infarction (STEMI), the number of vascular disease and underwent PCI were the independent risk factors of all-cause mortality [the odds ratio (OR) and 95% confidence interval (95%CI) were 1.644 (1.356-1.993), 11.794 (7.469-18.621), 2.449 (1.419-4.227), 1.334 (1.096-1.624), 0.391 (0.247-0.619), all P < 0.001]. Cox regression analysis showed that age, STEMI, the number of vascular disease and underwent PCI were independent risk factors of the occurrence of MACCE within 1 year after discharge [hazard ratio (HR) and 95%CI were 1.354 (1.205-1.521), 1.387 (1.003-1.916), 1.314 (1.155-1.495), 0.547 (0.402-0.745), all P < 0.05]. CONCLUSIONS: In the chest pain center model, compared with other age of ACS patients, the proportion of NSTEMI in elderly patients group aged ≥ 75 years old was higher, the proportion of PCI was lower, and the clinical outcome was worse. However, the prognosis of elderly patients receiving PCI treatment was better than the patients receiving conservative treatment.


Assuntos
Síndrome Coronariana Aguda , Intervenção Coronária Percutânea , Idoso , Dor no Peito/epidemiologia , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Clínicas de Dor , Estudos Retrospectivos , Fatores de Risco , Resultado do Tratamento
7.
Nutrients ; 13(2)2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33671493

RESUMO

A few studies suggested high stereo-specifically numbered (sn)-2 palmitate in a formula might favor the gut Bifidobacteria of infants. The initial colonization and subsequent development of gut microbiota in early life might be associated with development and later life functions of the central nervous system via the microbiota-gut-brain axis, such as children with autism. This study aims to assess the hypothesized effect of increasing the amount of palmitic acid esterified in the sn-2 position in infant formula on neurodevelopment in healthy full-term infants and to explore the association of this effect with the altered gut Bifidobacteria. One hundred and ninety-nine infants were enrolled in this cluster randomized clinical trial: 66 breast-fed (BF group) and 133 formula-fed infants who were clustered and randomly assigned to receive formula containing high sn-2 palmitate (sn-2 group, n = 66) or low sn-2 palmitate (control group, n = 67), where 46.3% and 10.3% of the palmitic acid (PA) was sn-2-palmitate, respectively. Infants' neurodevelopmental outcomes were measured by the Ages and Stages Questionnaire, third edition (ASQ-3). Stool samples were collected for the analysis of Bifidobacteria (Trial registration number: ChiCTR1800014479). At week 16, the risk of scoring close to the threshold for fine motor skills (reference: scoring above the typical development threshold) was significantly lower in the sn-2 group than the control group after adjustment for the maternal education level (p = 0.036) but did not differ significantly versus the BF group (p = 0.513). At week 16 and week 24, the sn-2 group (week 16: 15.7% and week 24: 15.6%) had a significantly higher relative abundance of fecal Bifidobacteria than the control group (week 16: 6.6%, p = 0.001 and week 24:11.2%, p = 0.028) and did not differ from the BF group (week 16: 14.4%, p = 0.674 and week 24: 14.9%, p = 0.749). At week 16, a higher relative abundance of Bifidobacteria was associated with the decreased odds of only one domain scoring close to the threshold in the formula-fed infants group (odds ratio (OR), 95% confidence interval (CI): 0.947 (0.901-0.996)). Elevating the sn-2 palmitate level in the formula improved infants' development of fine motor skills, and the beneficial effects of high sn-2 palmitate on infant neurodevelopment was associated with the increased gut Bifidobacteria level.

8.
Mater Sci Eng C Mater Biol Appl ; 122: 111939, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33641927

RESUMO

Strontium loaded titania nanotube arrays (NTSr), as well as titania nanotube arrays (NT), have been regarded as effective coatings to promote bone regeneration on titanium implants, but an understanding of the full extent of early processes affected by such surface modifications is absent. To address this limitation, we performed RNA sequencing (RNA-seq) of Sprague-Dawley rat bone marrow mesenchymal stem cells (rBMMSCs) cultured on unmodified titanium sheets (Con), NT and NTSr specimens. By pairwise comparisons we found that NT and NTSr shared a majority of differentially expressed genes. The Gene Ontology (GO) analysis revealed that NT and NTSr up-regulated a bunch of genes that are annotated to the cytoskeleton. The results were supported by immunofluorescent, transmission electron microscopy (TEM) and western blotting assays. By inhibiting the cytoskeleton through pharmacological agents, the activities of alkaline phosphatase (ALP) on NT and NTSr were also suppressed. Informed by these results, we concluded that NT and NTSr specimens reorganized the cytoskeleton of cultured cells that may play a crucial role in osteogenic lineage commitment.

9.
Technol Cancer Res Treat ; 20: 1533033821995281, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33632085

RESUMO

OBJECTIVE: We aimed to investigate the diagnostic value of the vaginal microecology, serum miR-18a, and programmed death ligand-1 (PD-L1) for human papillomavirus (HPV)-positive cervical cancer. METHODS: Eighty-four patients with HPV-positive cervical cancer were assigned to the observation group, 107 HPV-positive patients without cervical cancer were assigned to the positive group, and 191 healthy women were assigned to the control group. Vaginal microecology and serum levels of miR-18a and PD-L1 on the surface of CD4+ and CD8+ T cells were compared among the 3 groups. The observation group was further divided into subgroups according to patients' characteristics for comparison. The diagnostic value of miR-18a and PD-L1 for HPV-positive cervical cancer was investigated. RESULTS: Women in the control group had better vaginal microecology and lower levels of miR-18a and PD-L1 than those in the observation and the positive groups (all P < 0.05). Compared with the positive group, the observation group had similar vaginal microecology (all P > 0.05) but higher levels of miR-18a and PD-L1 (all P < 0.05). Moreover, the patients at stage III had higher levels of miR-18a and PD-L1 than those at stage I and II (all P < 0.05). The values of area under the curve for miR-18a and PD-L1 in the diagnosis of HPV-positive cervical cancer were over 0.8 (all P < 0.001). CONCLUSION: Patients with HPV-positive cervical cancer have vaginal microbial dysbiosis and high serum levels of miR-18a and PD-L1. miR-18a and PD-L1 have diagnostic value for identifying HPV-positive cervical cancer.

10.
Neurol Res ; 43(4): 336-341, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33443462

RESUMO

Background The aims of this study were to determine the relationships between changes inlatency and amplitude of the P300 event-related potential component and cognitive impairmentsin patients after a transient ischemic attack (TIA) or a minor stroke and to assess thesuitability of the P300 for screening for cognitive impairments.Material and Method Sixty-five TIA or minor stroke patients diagnosed at the NeurologyDepartment of Beijing Tiantan Hospital, Capital Medical University from June 2015 toDecember 2016 and 30 healthy people evaluated in the same period were included. Allpatients were examing neuropsychological scales and event-related potentials within7 ± 3 days of onset of the disease. The TIA/minor stroke group was divided into normal cognition group(NC) and cognitive impairment group. The cognitive impairment group was further divided into vascular cognitive impairment with no dementia(VCIND) group and vascular dementia (VD) group to analyze the relationship between P300 latency.Results The P300 latency at each recording electrode was longer in the NC and VCIND groups than healthy control group (P < 0.001), the P300 latency of VCIND group longer than NC group (P < 0.001). When the P300-Fz latency cut-off value was 358.6 ms,the sensitivity for diagnosing cognitive impairment in patients after TIA/minor stroke was 0.875 and the specificity was 0.765.Conclusions The P300 latency delay can be used to detect cognitive impairments in patients after TIA/minor stroke and the P300-Fz latency is more sensitive for diagnosing cognitiveimpairments in TIA/minor stroke patients.

11.
Neurol Res ; 43(5): 406-411, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33455562

RESUMO

Objective: This study aims to discover whether insulin resistance is an independent predictor for antiplatelet drug resistance in patients with ischemic cerebrovascular disease.Methods: This study used a prospective cohort method. Patients diagnosed as minor ischemic stroke or transient ischemic attack (TIA) were enrolled successively. All patients have been administrated aspirin and/or clopidogrel and were tested for fasting glucose and insulin and platelet aggregation inhibition tests which was detected by light transmission aggregometry (LTA). The maximum platelet aggregation rate (AA) of ≥20% or the maximum platelet aggregation rate (ADP) of ≥50% was defined as antiplatelet drug resistance. Multivariable logistic regression was performed to estimate the association between HOMA-IR and antiplatelet drug resistance.Results: This study recruited successively 237 patients with mild-to-moderate ischemic stroke or TIA in Beijing Tiantan Hospital from 2018 to 2019. Of them 60 cases were recognized as having insulin resistance. There are 46 patients in insulin resistance group (76.7%) developed antiplatelet drug resistance, which was significantly more frequent than patients without insulin resistance (35%, P < 0.0001). Insulin resistance was an independent risk factor for antiplatelet drug resistance in patients with recent ischemic stroke/TIA after adjusted for confounding factors (Odds Ratio 5.281; 95%CI, 2.15 to 13.01, P < 0.0001).Conclusions: Insulin resistance was an independent predictor for development of antiplatelet drug resistance in patients with recent minor ischemic stroke or TIA. More attention should be paid to recognize these patients and antithrombotic effect should be monitored when antiplatelet drugs were applied to these patients.

12.
Life Sci ; 269: 119064, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33460665

RESUMO

AIMS: Previous studies have uncovered the function of receptor-interacting protein kinase 1 (RIPK1) to mediate both cell survival and death. Moreover, RIPK1 modulates apoptosis and necroptosis depending on its activity, phosphorylation or ubiquitylation status. Many studies have explained the role or mechanism of RIPK1 in necroptosis. However, the role of RIPK1 has not been elucidated fully in human esophageal squamous cell carcinoma (ESCC) cells. MATERIALS AND METHODS: The protein and mRNA expression levels of RIPK1 in a panel of ESCC cell lines by Western blot and real-time quantitative reverse transcription PCR (qRT-PCR) were analyzed. MTS assay was used to examine cellular proliferation, flow cytometric analysis to detect apoptosis, mitochondrial membrane potential and reactive oxygen species production. ESCC cells with either inhibitor or overexpressed RIPK1were analyzed to determine cell proliferation, colony formation and apoptosis. Flow cytometry and western blotting assays were used to explore the underlying mechanism. KEY FINDINGS: In our study, RIPK1 expression was found to contribute significantly to cisplatin-induced apoptosis in the human ESCC cells. The reduced RIPK1 expression promoted cells proliferation and overexpressed RIPK1 facilitated cell apoptosis. Mechanistic investigations have revealed that the inhibition of proliferation for RIPK1 in ESCC cells was regulated via activation of c-Jun NH2-terminal kinase signaling. Additionally, damages were observed in the mitochondrial membrane, depletion of ATP and increased generation in reactive oxygen species. SIGNIFICANCE: Our findings verified the evidence that RIPK1 can promote cell death in ESCC cells, with potential implications for activating c-Jun NH2-terminal kinase pathway as a novel approach to the disease.


Assuntos
Apoptose , Cisplatino/farmacologia , Neoplasias Esofágicas/patologia , Carcinoma de Células Escamosas do Esôfago/patologia , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Proteína Serina-Treonina Quinases de Interação com Receptores/metabolismo , Antineoplásicos/farmacologia , Proliferação de Células , Neoplasias Esofágicas/tratamento farmacológico , Neoplasias Esofágicas/metabolismo , Carcinoma de Células Escamosas do Esôfago/tratamento farmacológico , Carcinoma de Células Escamosas do Esôfago/metabolismo , Humanos , Proteína Serina-Treonina Quinases de Interação com Receptores/genética , Células Tumorais Cultivadas
13.
BMC Surg ; 21(1): 57, 2021 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-33485329

RESUMO

BACKGROUND: To determine the standard remnant liver volume (SRLV) threshold to avoid postoperative hepatic insufficiency inpatients in different stages of hepatic fibrosis who undergo right hemi-hepatectomy. METHODS: Data for 85 patients at our single medical center were analysed prospectively to examine whether the following factors differed significantly between those who experienced postoperative hepatic insufficiency and those who did not: height, prothrombin time, remnant liver volume, SRLV or hepatic fibrosis stage. RESULTS: Logistic regression showed SRLV and hepatic fibrosis stage to be independent risk factors for postoperative hepatic insufficiency. The threshold SRLV for predicting insufficiency was 203.2 ml/m2 across all patients [area under receiver operating characteristic curve (AUC) 0.778, sensitivity 66.67%, specificity 83.64%, p<0.0001), 193.8 ml/m2 for patients with severe hepatic fibrosis (AUC 0.938, sensitivity 91.30%, specificity 85.71%, p<0.0001), and 224.3 ml/m2 for patients with cirrhosis (AUC 0.888, sensitivity 100%, specificity 64.29%, p<0.0001). CONCLUSIONS: Right hemi-hepatectomy may be safer in Chinese patients when the standard remnant liver volume is more than 203.2 ml/m2 in the absence of hepatic fibrosis or cirrhosis, 193.8 ml/m2 in the presence of severe hepatic fibrosis or 224.3 ml/m2 in the presence of cirrhosis.


Assuntos
Carcinoma Hepatocelular , Hepatectomia/efeitos adversos , Cirrose Hepática , Neoplasias Hepáticas , Fígado , Adulto , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/patologia , Carcinoma Hepatocelular/cirurgia , Simulação por Computador , Feminino , Hepatectomia/métodos , Insuficiência Hepática/etiologia , Insuficiência Hepática/prevenção & controle , Humanos , Imageamento Tridimensional , Fígado/diagnóstico por imagem , Fígado/patologia , Fígado/cirurgia , Cirrose Hepática/complicações , Cirrose Hepática/diagnóstico por imagem , Cirrose Hepática/patologia , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/patologia , Neoplasias Hepáticas/cirurgia , Masculino , Pessoa de Meia-Idade , Tamanho do Órgão , Estudos Prospectivos , Padrões de Referência , Valores de Referência , Fatores de Risco , Tomografia Computadorizada por Raios X
14.
Int J Clin Exp Pathol ; 13(11): 2870-2888, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33284868

RESUMO

OBJECTIVE: To study the ganglioside intake of lactating mothers and its effect on the breast milk and infants. METHODS: The related information of mothers and infants was obtained by questionnaire survey, including the recipe, family information, and so on. The content of gangliosides in the mothers' food and breast milk was tested by HPLC-MS. The intake of gangliosides for infants was recorded and calculated. Then the dynamic changes of the content of gangliosides in breast milk and the impact on the development of infants were evaluated. RESULTS: GD3 was rich in milk and dairy products. The average intake of gangliosides for lactating mothers was 6.33 mg/day, of which GM3 was 3.02 mg/day and GD3 was 1.51 mg/day. The main food sources of gangliosides were meat (46.6%), eggs (26.6%), and dairy products (18.9%). The average content of gangliosides in breast milk was 9.58 mg/L. The content in 0-7 days after delivery (15.95 mg/L) was the highest, and then gradually decreased with time, getting the lowest in 6 months after delivery (6.47 mg/L). GM3 and GD3 were the two main types in breast milk. The average milk intake of infants under 6 months gradually increased from 570 mL to 1367 mL, and the daily intake of gangliosides was relatively stable, with a median of 6.4 mg. There was no significant relationship between the intake of gangliosides and physical development in infants. CONCLUSION: This study is the first to report the dietary ganglioside intake of Chinese city mothers. This study is also the first to indirectly infer the demand of infant ganglioside by detecting the components of breast milk. It will accumulate basic data for improving the diet of Chinese mothers and the recommended amount of infant nutrients.

15.
Anim Biotechnol ; : 1-9, 2020 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-33302802

RESUMO

To investigate the effect of sea buckthorn puree consumption on reducing blood lipid and other risk factors of CVD. A total of 111 patients with hypercholesteromia who were treated with 90 ml sea buckthorn puree or placebo for 90 days were enrolled in this trial. Physical examination and analysis of lipid markers, hsCRP concentrations and cell adhesion protein concentrations with fasting blood samples were performed at 0-day, 45-day and 90-day after treatment. We found that the sea buckthorn puree did not affect the levels of serum total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) and triglyceride (TG). However, the level of HDL-C was decreased by sea buckthorn puree in a short period (first 6 weeks), which was increased in the last 6 weeks (p < 0.05). Compared with placebo, diastolic blood pressure (DBP) was decreased after taking sea buckthorn puree (p > 0.05). There was moderately decreased in hsCRP concentration in sea buckthorn group. Nevertheless, there was no correlation between changes in ICAM-1 and VCAM-1 concentration. In conclusion, long term consumption of sea buckthorn puree has anti-inflammatory and anti-hypertensive effects on hypercholesterolemia in hypercholesterolemic patients. However, it did not translate into an effect on the concentration of lipid markers in the circulation.

16.
World J Gastroenterol ; 26(43): 6810-6821, 2020 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-33268963

RESUMO

BACKGROUND: The complications acute lung injury and acute kidney injury caused by severe inflammation are the main reasons of high mortality of severe acute pancreatitis (SAP). These two complications can both lead to water metabolism and acid-base balance disorders, which could act as additional critical factors affecting the disease trend. Aquaporins (AQPs), which can regulate the transmembrane water transport, have been proved to participate in the pathophysiological process of SAP and the associated complications, such as acute lung injury and acute kidney injury. Thus, exploring herbs that can effectively regulate the expression of AQP in SAP could benefit the prognosis of this disease. AIM: To determine whether Yue-Bi-Tang (YBT) can regulate the water metabolism in rats with severe acute pancreatitis via regulating the expression of aquaporins. METHODS: Sprague-Dawley rats were randomly divided into three groups, sham operation group (SOG), model group (MG), and treatment group (TG). SAP was induced with 3.5% sodium taurocholate in the MG and TG. Rats in the TG were administered with YBT while SOG and MG rats were given the same volume of saline. Blood and tissue samples were harvested to detect serum inflammatory cytokines, histopathological changes, malondialdehyde and superoxide dismutase in the lung, and protein and mRNA expression of kidney injury molecule-1, α-smooth muscle actin, and vimentin in the kidney, and AQP1 and 4 in the lung, pancreas, and kidney. RESULTS: The serum interleukin-10, tumor necrosis factor α, and creatinine levels were higher in the MG than in the SOG. Tumor necrosis factor α level in the TG was lower than that in the MG. Malondialdehyde level in lung tissues was higher than in the SOG. The pathological scores and edema scores of the pancreas, lung, and kidney tissues in the MG were all higher than those in the SOG and TG. The protein expression of AQP4 in lung tissues and AQP1 in kidney tissues in the MG were higher than those in the SOG and TG. The expression of vimentin was significantly higher in the MG than in the SOG. The expression of AQP1 mRNA in the lung and kidney, and AQP4 mRNA in the kidney was up-regulated in the MG compared to the SOG. CONCLUSION: YBT might regulate water metabolism to reduce lung and kidney edema of SAP rats via decreasing AQP expression, and alleviate the tissue inflammatory injury.

17.
J Diabetes Investig ; 2020 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-33249775

RESUMO

AIMS: The predictive value of admission hyperglycemia in the long-term prognosis of AMI patients is still controversial. We aim to investigate this value based on the diabetic status. METHODS: We performed a multicenter, retrospective study on 1,288 AMI patients enrolled in 11 hospitals between March 2014 and June 2019 in Chengdu. The patients were classified into diabetics and non-diabetics groups, each was further divided into: hyperglycemia and non-hyperglycemia subgroups, according to the optimal cut-off value of the blood glucose to predict all-cause mortality during follow-up. The endpoints were all-cause death and major adverse cardiovascular and cerebrovascular events (MACCE), including all-cause death, non-fatal myocardial infarction, vessel revascularization and non-fatal stroke. RESULTS: In the follow-up of 15 months, we observed 210 (16.3%), 6 (0.5%), 57 (4.4%) and 34 (2.6%) cases of death, non-fatal MI, revascularization and non-fatal stroke, respectively. The optimal cut-off values of admission blood glucose for diabetics and non-diabetics to predict all-cause mortality during follow-up were 14.80 mmol/L and 6.77 mmol/L, respectively. We divided diabetics (n=331) into hyperglycemia (n=92) and non-hyperglycemia (n=239), and non-diabetics (n=897) into hyperglycemia (n=425) and non-hyperglycemia (n=472). The cumulative rates of all-cause death and MACCE of the patients in each hyperglycemia group was higher than that in the corresponding non-hyperglycemia group (p-value < 0.001). In non-diabetics, admission hyperglycemia was an independent predictor of all-cause mortality and MACCE. CONCLUSION: Admission hyperglycemia was an independent predictor for long-term prognosis in non-diabetic AMI patients.

18.
Neurol Sci ; 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33179196

RESUMO

OBJECTIVE: Diabetes mellitus (DM) had been discovered as an independent risk factor for high on-treatment platelet reactivity (HPR) in patients with ischemic stroke. However, studies on the relationship between prediabetes and the occurrence of HPR remain scarce. This study is aimed at clarifying the association between prediabetes and HPR among patients with recent ischemic stroke or transient ischemic stroke (TIA). METHODS: Patients with ischemic stroke or TIA within 90 days after onset were recruited consecutively. All patients were divided into three groups: DM, prediabetes, and normal glucose tolerance according to fasting glucose, HbA1c, or OGTT. Three months later, all patients were performed platelet aggregation inhibition test and screened for high on-treatment platelet reactivity. The effect of prediabetes on HPR was analyzed in a multivariable logistic regression model. RESULTS: This study recruited 237 patients with ischemic stroke or TIA, including 57 cases with prediabetes, 108 with DM, and 72 with normal glucose tolerance. Aspirin and/or clopidogrel resistance was discovered in 28 cases among prediabetes group, which was significantly more frequent than normal glucose tolerance group(49.1% versus 33.3%, P = 0.046). After adjusting for confounding factors, prediabetes was found as an independent risk factor for high on-treatment platelet reactivity (HPR) among patients with recent ischemic stroke or TIA (odds ratio 2.92; 95% CI, 1.29 to 6.63, P = 0.01). CONCLUSIONS: Prediabetes was an independent risk factor for high on-treatment platelet reactivity in patients with recent ischemic cerebrovascular disease. Patients with prediabetes should be highlighted for the efficacy test of antiplatelet drugs.

19.
Biomed Mater ; 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33036006

RESUMO

Micro/nano-topography (MNT) promotes osteogenic differentiation of stem cells, but the topographical signaling transduction mechanisms remain unclear. We have confirmed that MNT as a stressor triggers endoplasmic reticulum (ER) stress and activates unfolded protein response (UPR) in rBMMSCs, and such topography-induced ER stress improves osteogenic differentiation. In order to reveal the influence of nanotube dimension on ER stress, MNTs containing vertically oriented TiO2 nanotubes with defined diameters between 30 and 100 nm were fabricated on pure titanium (Ti) foils, then ER stress and osteogenic differentiation of cells was systematically investigated. After cultivating for 12 h, cells on MNTs showed gross distortion of rough ER morphology that containing the electron-dense material through the transmission electron microscopy (TEM) examination, and the expansion of the ER lumen became more pronounced as the dimension of nanotubes increased. Additionally, PCR and western blotting showed that the ER stress-related gene the ER chaperone 78 kDa glucose-regulated protein or immunoglobulin binding protein (GRP78/BiP) was up-regulated dependent on the nanotube dimension, which was in line with the osteogenic induction capabilities of MNTs. Our findings further revealed the mechanism for topographical cues modulating osteogenic differentiation of cells, which may provide an innovated criterion of the optimal design of implant surface topography.

20.
Polymers (Basel) ; 12(10)2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33066012

RESUMO

Considerable attention has been devoted to the in-situ deposition of zinc oxide (ZnO) nanowires (ZnO-NWs) on the surface of organic supports, due to their very wide applications in superhydrophobicity, UV shielding, and nanogenerators. However, the poor interfacial bond strength between ZnO-NWs and its support limits their applications. Herein, we developed a facile process to grow robust ZnO-NWs on a polyethylene terephthalate (PET) fabric surface through simultaneous radiation-induced graft polymerization, hydrothermal processing, and in-situ nano-packaging; the obtained materials were denoted as PDMS@ZnO-NWs@PET. The introduction of an adhesion and stress relief layer greatly improved the attachment of the ZnO-NWs to the support, especially when the material was subjected to extreme environment conditions of external friction forces, strong acidic or alkaline solutions, UV-irradiation and even washing with detergent for a long time. The PDMS@ZnO-NWs@PET material exhibited excellent UV resistance, superhydrophobicity, and durability. The ZnO-NWs retained on the fabric surface even after 30 cycles of accelerated washing. Therefore, this process can be widely applied as a universal approach to overcome the challenges associated with growing inorganic nanowires on polymeric support surfaces.

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