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1.
World J Surg Oncol ; 19(1): 296, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34635107

RESUMO

PURPOSE: To investigate the lymph node metastasis pattern and significance of dissection of the left gastric artery lymph nodes in radical en bloc esophagectomy for esophageal squamous carcinomas based on the lymphatic drainage pathway revealed by carbon nanoparticle labeling. MATERIALS AND METHODS: Patients who underwent en bloc esophagectomy endoscopically were retrospectively enrolled. Carbon nanoparticles were injected in the submucosa of upper thoracic esophagus to label the relevant draining lymph nodes. The clinical data, lymph nodes dissected, surgical technique, and complications were analyzed. RESULTS: En bloc esophagectomy was successful in all 179 patients. Metastases to the left gastric artery lymph nodes were positive in 42 patients (23.5%) but negative in 137 (76.5%). The left gastric lymph nodes were labeled, whereas no celiac lymph nodes were labeled by carbon nanoparticles. A total of 4652 lymph nodes were resected, with 26 lymph nodes per patient. Seventy-three patients had lymph node metastasis (73/179). Seventeen patients had metastasis to the recurrent laryngeal nerve lymph nodes (9.5%). The metastasis rate of the lower thoracic esophageal cancer to the left gastric artery lymph nodes was 37.0%, significantly greater than that at the middle (15.4%) or upper (6.7%) thoracic segment. The lymph node metastasis rate was significantly (P < 0.05) increased with the length of the cancerous lesion, infiltration depth, and poor differentiation. Univariate analysis revealed that the metastasis rate to the left gastric artery lymph nodes was significantly (P < 0.05) associated with paraesophageal lymph node metastasis, para-cardial lymph metastasis, and TNM classification. Multivariate analysis indicated that cancer location (odds ratio 8.32, 95% confidence interval 2.12-32.24) was significantly (P < 0.05) associated with metastasis to the left gastric artery lymph nodes, with the cancer at the middle and lower thoracic segments significantly more than in the upper thoracic segment. CONCLUSION: Certain patterns exist in lymph node metastasis of esophageal cancer, and in radical esophagectomy of esophageal cancers, dissection of the left gastric artery lymph nodes is necessary to prevent possible residual or metastasis of esophageal squamous carcinomas based on the lymphatic drainage pathway of esophageal carcinomas demonstrated by carbon nanoparticle labeling.


Assuntos
Neoplasias Esofágicas , Esofagectomia , Dissecação , Neoplasias Esofágicas/cirurgia , Artéria Gástrica , Humanos , Excisão de Linfonodo , Linfonodos/cirurgia , Metástase Linfática , Prognóstico , Estudos Retrospectivos
2.
Eur J Med Genet ; 64(12): 104362, 2021 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-34637946

RESUMO

Autosomal recessive deafness-102 (DFNB102), a new profound prelingual non-syndromic hearing loss, is caused by mutations in the EPS8 gene. To date, only three such consanguineous families with three different homozygous variants in EPS8 have been reported. Here, we report the fourth case from a non-consanguineous Chinese family, an 11-month-old male infant presented with congenital profound non-syndromic hearing loss. Trio whole-exome sequencing initially identified the patient with a novel seemingly homozygous splicing variant NM_004447.5: c.1435-2A > T in intron 14 of the EPS8 gene and was inherited from his father; further CNVs analysis identified a novel 65.9 kb intragenic deletion and was inherited from his mother. The deletion is covering intron 14 that could account for the apparent homozygosity of the patient. In vitro splicing assay showed the variant c.1435-2A > T creates a new donor site at position c.1443, which is predicted to produce a stop codon after 14 additional amino acids (p.His479Cysfs*14). Furthermore, quantitative allele-specific expression assay showed that relative EPS8 gene expression in the patient significantly decreased (0-fold for the wild-type transcript and 0.25-0.27-fold for the mutant transcript) compared to the control (P < 0.05), indicating the pathogenicity of the identified variants. Overall, our study provides additional evidence that EPS8 is a causative gene for DFNB102 and highlights the clinical utility of simultaneous analysis of CNVs and SNVs to avoid potential errors in the diagnosis and interpretation of patients with apparent homozygosity.

3.
Acad Radiol ; 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34649778

RESUMO

RATIONALE AND OBJECTIVES: Combined immune and anti-angiogenic treatment has shown promising results for unresectable hepatocellular carcinoma (HCC), but with a high risk of early progression. In this study, we aimed to investigate whether pre-treatment magnetic resonance imaging (MRI) features and MRI-based nomogram could predict the risk of disease progression of unresectable HCC after first-line lenvatinib/anti-PD-1 antibody therapy. MATERIALS AND METHODS: Thirty-seven HCC participants with qualified pre-treatment contrast-enhanced MRI were enrolled. All patients received combined lenvatinib and anti-PD-1 antibody treatment. Progression free survival rate was analyzed using the Kaplan-Meier method. Potential clinical-radiological risk factors for progression were analyzed using the log-rank tests and Cox regression model. The performance of MRI-based nomogram was evaluated based on C-index, calibration, and decision curve analyses. RESULTS: The 6-month and 12-month cumulative progression free survival rates were 59.5% (95% confidence interval (CI), 43.6%-75.4%) and 48.0% (95% CI, 31.7%-64.3%). On multivariate analysis, no or incomplete tumor capsule (hazard ratio (HR) = 15.215 [95% CI 2.707-85.529], p = 0.002), heterogeneous signal on T2-weighted imaging (HR = 28.179 [95% CI 2.437-325.838]; p = 0.008) and arterial contrast-to-noise ratio ≤95.45 (HR = 5.113 [95% CI 1.538-17.00]; p = 0.008) were independent risk factors for disease progression. Satisfactory predictive performance of the nomogram incorporating the three independent imaging features was obtained with a C-index value of 0.880 (95% CI 0.824-0.937), and the combined nomogram had more favorable clinical prediction performance than any single feature. CONCLUSION: MRI features can be considered effective predictors of disease progression for unresectable HCC with first-line lenvatinib plus anti-PD-1 antibody therapy, and the combined MRI-based nomogram achieved a superior prognostic model, which may help to identify appropriate candidates for the therapy.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34487492

RESUMO

We put forward a focusing formula to describe the guided waves periodical focusing phenomenon, which, apart from being a fundamental problem in the guided waves propagation in the pipes, has essential applications in the field of nondestructive evaluation. Due to the partial circumferential loads or non-axisymmetric defects, the guided waves are not only in the zeroth-order axisymmetric forms but also in the higher-order non-axisymmetric forms. When multiple orders of the same mode exist simultaneously, the angular profile is adopted to describe the circumferential energy distribution of the superposed wave field. However, the angular profile varies in the propagation process. In our finding, this variation is periodic, meaning that the circumferential energy will repeat the process of dispersing and focusing. Thus, we put forward a focusing formula to describe the phenomenon. The proposed formula indicates that the angular profile varies periodically with the ratio of propagation distance to wave number, and this period is only related to the pipe radius. Thus, three factors, including propagation distance, excitation frequency, and pipe radius, will affect the angular profile. Moreover, we established an experimental system to verify this phenomenon, based on which we have designed three groups of experiments to investigate these three factors. The experimental results are in good agreement with the theoretical predictions.

5.
J Environ Sci (China) ; 108: 96-106, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34465441

RESUMO

Hydrodechlorination is a promising technology for the remediation of water body contaminated with trichloroethylene (TCE). In this work, the liquid-phase hydrogenation of TCE by Raney Ni (R-Ni) and Pd/C under an open system have been studied, in which nascent H2 (Nas-H2) generated in situ from the cathode acted as a hydrogen source. Experimental results showed that TCE was completely eliminate from the solution through the synergistic effects of hydrodechlorination and air flotation due to the formation of continuous micro/nano-sized Nas-H2 bubbles from the cathode. Furthermore, the effects of inorganic anions and organic solvents on R-Ni and Pd/C hydrogenation activity were investigated, respectively. The results showed that NO3- and acetonitrile can form a competitive reaction with TCE; Sulfur with lone-pair electrons will cause irreversible poisoning to these two catalysts, and have a stronger inhibitory effect on Pd/C. This work helps to realize the separation of volatile halogenated compounds from water environment and provides certain data support for the choice of catalyst in the actual liquid-phase hydrogenation system.


Assuntos
Tricloroetileno , Catálise , Hidrogenação , Solventes , Enxofre
6.
Magn Reson Imaging ; 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34506910

RESUMO

PURPOSE: To assess the clinical potential of a set of new diffusion parameters (D, ß, and µ) derived from fractional order calculus (FROC) diffusion model in predicting microvascular invasion (MVI) of hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Between January 2019 to November 2020, a total of 63 patients with HCC were enrolled in this study. Diffusion-weighted images were acquired by using ten b-values (0-2000 s/mm2). The FROC model parameters including diffusion coefficient (D), fractional order parameter (ß), a microstructural quantity (µ) together with a conventional apparent diffusion coefficient (ADC) were calculated. Intraclass coefficients were calculated for assessing the agreement of parameters quantified by two radiologists. The differences of these values between the MVI-positive and MVI-negative HCC groups were compared by using independent sample t-test or the Mann-Whitney U test. Then the parameters showing significant differences between subgroups, including the ß and D, were integrated to develop a comprehensive predictive model via binary logistic regression. The diagnostic performance was evaluated by receiver operating characteristic (ROC) analysis. RESULTS: Among all the studied diffusion parameters, significant differences were found in D, ß, and ADC between the MVI-positive and MVI-negative groups. MVI-positive HCCs showed significantly higher ß values (0.65 ± 0.17 vs. 0.51 ± 0.13, P = 0.001), along with lower D values (0.84 ± 0.11 µm2/ms vs. 1.03 ± 0.13 µm2/ms, P < 0.001) and lower ADC values (1.38 ± 0.46 µm2/ms vs. 2.09 ± 0.70 µm2/ms, P < 0.001) than those of MVI-negative HCCs. According to the ROC analysis, the combination of D and ß demonstrated the largest area under the ROC curve (0.920) compared with individual parameters (D: 0.912; ß: 0.733; and ADC: 0.831) for differentiating MVI-positive from MVI-negative HCCs. CONCLUSIONS: The FROC parameters can be used as noninvasive quantitative imaging markers for preoperatively predicting the MVI status of HCCs.

7.
Sci Total Environ ; 805: 150336, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34537699

RESUMO

In materials science, "green" synthesis has gotten a lot of interest as a reliable, long-lasting, and ecofriendly way to make a variety of materials/nanomaterials, including metal/metal oxide nanomaterials. To accommodate various biological materials, green synthesis of metallic nanoparticles has been used (e.g., bacteria, fungi, algae, and plant extracts). In this work, Shewanella oneidensis MR-1 was used to biosynthesize palladium nanoparticles (bioPd) under aerobic conditions for the Cr(VI) bio-reduction. The size and distribution of bio-Pd are controlled by adjusting the ratio of microbial biomass and palladium precursors. The high cell: Pd ratio has the smallest average particle size of 6.33 ± 1.69 nm. And it has the lowest electrocatalytic potential (-0.132 V) for the oxidation of formic acid, which is 0.158 V lower than commercial Pd/C (5%). Our results revealed that the small size and uniformly distributed extracellular bio-Pd could achieve completely catalytic reduction of 200 mg/L Cr(VI) solution within 10 min, while the commercial Pd/C (5%) need at least 45 min. The bio-Pd materials maintain a high reduction during five cycles. Microorganisms play an important role in the whole process, which can fully disperse palladium nanoparticles, completely reduce Cr(VI), and effectively adsorb Cr(III). This work expands our understanding and provides a reference for the design and development of efficient and green bio-Pd catalysts for environmental pollution control under simple and mild conditions.

8.
J Interferon Cytokine Res ; 41(9): 319-328, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34543127

RESUMO

Although high-mobility group box-1 (HMGB1) is related to the persistent intestinal inflammation in the development of necrotizing enterocolitis (NEC), the role of HMGB1 in the regulation of the intestinal microcirculation in NEC is not well understood. Therefore, we investigated the mechanism(s) by which HMGB1 regulates the generation of the following vasodilatory signals during the development of NEC: endothelial nitric oxide synthase (eNOS) and nitric oxide (NO). Experimental NEC was induced in full-term C57BL/6 mouse pups through the formula gavage and hypoxia technique. The blockade of HMGB1 was achieved with a subcutaneous injection of anti-HMGB1 antibody. Intestinal tissues and blood samples were collected at predetermined time points for the assessment of intestinal microcirculation, lipid peroxidation levels, and evaluation of eNOS activation. We found elevations in HMGB1 expression as early as 12 h after induction of NEC stress, which preceded intestinal injury. Treatment of mouse pups with HMGB1 neutralizing antibody attenuated the intestinal microvascular features and symptoms of NEC, but this improvement was not found in the eNOS knockout mice, suggesting that HMGB1 inhibition increased intestinal microcirculatory perfusion in an eNOS-dependent manner. Moreover, HMGB1 inhibition rescued NO production and eliminated O2•- production in experimental NEC mice through eNOS activation. These data indicate that excessive HMGB1 signaling is associated with the pathogenesis of NEC, suggesting that HMGB1 inhibition might be a promising strategy for NEC treatment.

9.
Environ Pollut ; : 118229, 2021 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-34582922

RESUMO

Polychlorinated biphenyls (PCBs) are persistent organic pollutants in environments, and they can negatively affect aquatic animal health. After 7 days of PCBs exposure, the activities of catalase, phenoloxidase, and superoxide dismutase and the total hemocyte count in the haemolymph were significantly decreased and the reactive oxygen species (ROS) content and phagocytic rate of hemocytes were significantly increased in mud crab Scylla paramamosain. Additionally, serum lysozyme, glutathione, glutathione-S-transferase, and glutathione peroxidase activities were significantly down-regulated in mud crab after PCBs exposure. The survival rate of crab hemocytes significantly declined as the PCBs concentration increased, indicating that PCBs had a cytotoxic effect on hemocytes. Exposure to increasing concentrations of PCBs also increased the degree of DNA damage in crab hemocytes. After PCBs exposure, the expression levels of P53 and caspase-3 in hemocytes were significantly up-regulated, which suggests that apoptosis was occurring. The apoptosis rate of hemocytes was up-regulated as the PCBs concentration increased, indicating that apoptosis was induced by the PCBs-activated caspase-3 pathway. These data suggest that exposure to PCBs hampered the immune response of mud crabs, most likely by (1) inducing ROS, causing DNA damage, and reducing the viability of hemocytes, (2) reducing the activities of antioxidant enzymes, and (3) inducing phagocytosis and apoptosis of hemocytes. And the final result of PCBs-induced immunotoxicity to mud crabs is the reduced bacterial disease resistance and survival rate of crabs under Vibrio alginolyticus challenge.

10.
Fish Shellfish Immunol ; 118: 205-212, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34517138

RESUMO

In recent years, the use of natural products with immune-stimulating and antimicrobial properties has attracted increasing attention in aquaculture researches. In our study, the effect of diet supplemented with quercetin, a flavonoid commonly found in some types of plants substance on the innate immune response and disease resistance in crayfish (Procambarus clarkii) against white spot syndrome virus (WSSV) is reported. It was found that dietary 40 mg/kg quercetin significantly reduced the mortality of crayfish and WSSV copy number after WSSV challenge. Dietary quercetin increased catalase (CAT), and lysozyme (LZM) activity in crayfish. Dietary quercetin increased the expression of NF-κB, anti-lipopolysaccharide factor (ALF) and toll-like receptor (TLR) genes in crayfish. The apoptosis rate of hemocyte was increased by quercetin supplement in crayfish. Our results suggest that dietary quercetin may affect the innate immunity of crayfish and protect crayfish from WSSV infection.

11.
Acad Radiol ; 2021 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-34429260

RESUMO

RATIONALE AND OBJECTIVES: Accurately staging liver fibrosis is of great clinical significance. We aimed to evaluate the clinical potential of the non-Gaussian fractional order calculus (FROC) diffusion model in staging liver fibrosis. MATERIALS AND METHODS: A total of 82 patients with chronic hepatitis B (CHB) were included in this prospective study. Diffusion weighted imaging (DWI)-derived parameters including the diffusion coefficient (D), fractional order parameter (ß) and microstructural quantity (µ) sourced from FROC-DWI, and apparent diffusion coefficient (ADC) derived from mono-exponential DWI, as well as the aspartate aminotransferase-to-platelet ratio index (APRI) and fibrosis-4 (FIB-4) were calculated. Their correlations with fibrosis stages and the diagnostic efficacy in predicting liver fibrosis were assessed and compared. RESULTS: D (r = -0.667), ß (r = -0.671), µ (r = -0.481), and ADC (r = -0.665) displayed significant correlations with fibrosis stages (p < 0.001). D, ß and ADC (p < 0.01) were independently associated with fibrosis; and compared to inflammatory activity, fibrosis was the independent factor significantly correlated with D, ß and ADC (p < 0.001). There were no significant differences between the area under curves of D, ß, µ or their combinations and ADC for predicting different fibrosis stages (p > 0.05). The diagnostic performance of the combined index with four diffusion metrics was better than D, ß, µ or ADC used alone (p < 0.05) as well as APRI or FIB-4 (p < 0.01) in fibrosis staging. CONCLUSION: FROC-DWI was valuable in staging liver fibrosis in patients with CHB, but there were no significant differences between the FROC-DWI parameters and the classical ADC. However, the combined DWI-derived index including D, ß, µ and ADC offered the best diagnostic efficacy and may serve as a reliable tool for fibrosis evaluation, superior to APRI and FIB-4.

12.
Artigo em Inglês | MEDLINE | ID: mdl-34339372

RESUMO

This paper investigates adaptive streaming of one or multiple tiled 360 videos from a multi-antenna base station (BS) to one or multiple single-antenna users, respectively, in a multi-carrier wireless system. We aim to maximize the video quality while keeping rebuffering time small via encoding rate adaptation at each group of pictures (GOP) and transmission adaptation at each (transmission) slot. To capture the impact of field-of-view (FoV) prediction, we consider three cases of FoV viewing probability distributions, i.e., perfect, imperfect, and unknown FoV viewing probability distributions, and use the average total utility, worst average total utility, and worst total utility as the respective performance metrics. In the single-user scenario, we optimize the encoding rates of the tiles, encoding rates of the FoVs, and transmission beamforming vectors for all subcarriers to maximize the total utility in each case. In the multi-user scenario, we adopt rate splitting with successive decoding and optimize the encoding rates of the tiles, encoding rates of the FoVs, rates of the common and private messages, and transmission beamforming vectors for all subcarriers to maximize the total utility in each case. Then, we separate the challenging optimization problem into multiple tractable problems in each scenario. In the single-user scenario, we obtain a globally optimal solution of each problem using transformation techniques and the Karush-Kuhn-Tucker (KKT) conditions. In the multi-user scenario, we obtain a KKT point of each problem using the concave-convex procedure (CCCP). Finally, numerical results demonstrate that the proposed solutions achieve notable gains in quality, quality variation, and rebuffering time over existing schemes in all three cases. To the best of our knowledge, this is the first work revealing the impact of FoV prediction on the performance of adaptive streaming of tiled 360 videos.

13.
J Int Med Res ; 49(7): 3000605211029521, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34334005

RESUMO

OBJECTIVE: This study aimed to explore the potential molecular mechanism of allergic rhinitis (AR) and identify gene signatures by analyzing microarray data using bioinformatics methods. METHODS: The dataset GSE19187 was used to screen differentially expressed genes (DEGs) between samples from patients with AR and healthy controls. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were applied for the DEGs. Subsequently, a protein-protein interaction (PPI) network was constructed to identify hub genes. GSE44037 and GSE43523 datasets were screened to validate critical genes. RESULTS: A total of 156 DEGs were identified. GO analysis verified that the DEGs were enriched in antigen processing and presentation, the immune response, and antigen binding. KEGG analysis demonstrated that the DEGs were enriched in Staphylococcus aureus infection, rheumatoid arthritis, and allograft rejection. PPI network and module analysis predicted seven hub genes, of which six (CD44, HLA-DPA1, HLA-DRB1, HLA-DRB5, MUC5B, and CD274) were identified in the validation dataset. CONCLUSIONS: Our findings suggest that hub genes play important roles in the development of AR.


Assuntos
Biologia Computacional , Rinite Alérgica , Perfilação da Expressão Gênica , Ontologia Genética , Humanos , Mapas de Interação de Proteínas , Rinite Alérgica/genética
14.
Lung Cancer ; 159: 111-116, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34325317

RESUMO

OBJECTIVE: The association between the morphological characteristics and survival outcome of lung cancer associated with cystic airspaces (LCCAs) is unclear due to rarity of this disease. The current study attempted to compare the survival outcome between LCCAs and non-LCCAs and investigate the correlation between imaging features and prognosis of LCCA. METHOD: Of 10,835 patients diagnosed with non-small cell lung carcinoma (NSCLC) between January 2015 and December 2016, 123 patients with LCCA were included. The non-LCCA group comprised 3136 patients with primary solitary adenocarcinoma or squamous cell lung cancer. Propensity score matching (PSM) was performed for age, sex, tumor size, tumor stage, and lymph node involvement in a 1:1 ratio between the LCCAs and non-LCCAs, and the correlation between radiological features and recurrence-free survival (RFS) was analyzed. RESULT: The computed tomography (CT) lesion size was found to be higher in all LCCA subtypes, particularly in Type III (a cystic airspace with a mural nodule) and Type IV (mixed) LCCAs (3.09 and 3.65 cm, respectively), than in non-LCCAs (2 cm) after PSM. Three-year RFS in the LCCA group was higher than in the non-LCCA group (Type I- IV LCCAs: 100%, 84%, 77% and 83%, respectively vs. non-LCCAs: 77%). However, statistically significant difference was only found in comparison between LCCA Type I (thin-walled) and non-LCCA groups (P = 0.026). Type III lung cancer exhibited the worst survival among all four LCCA subtypes. CONCLUSIONS: The CT lesion size and pathologic tumor size varied significantly across LCCAs. Type I LCCAs exhibited better survival than non-LCCAs, whereas Type III LCCAs exhibited the worst survival rate among the four LCCA subtypes.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Carcinoma Pulmonar de Células não Pequenas/complicações , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Carcinoma Pulmonar de Células não Pequenas/patologia , Humanos , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/patologia , Estadiamento de Neoplasias , Prognóstico , Pontuação de Propensão , Estudos Retrospectivos
15.
ACS Appl Mater Interfaces ; 13(29): 34829-34842, 2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34264633

RESUMO

To develop multifunctional protein imprinted materials, a cobalt-iron double ion-BSA directional chelation-assisted thermo-sensitive surface-imprinted hollow nanocage (Co-Fe@CBMA-MIPs) with excellent specificity is developed on the surface of ZIF-67@Co-Fe in this study by synergizing the advantages of surface imprinting, metal ion chelation, anti-protein adsorption segments, and thermo-sensitive components. Beyond previous research, well-designed multifunctional protein-imprinted materials possess high binding capacity, fast adsorption kinetics, and outstanding selectivity. When the adsorption is carried out at 32 °C, the adsorption capacity of Co-Fe@CBMA-MIPs for BSA reaches 520.35 mg/g within 50 min. The imprinting factor is 8.55. The selectivity factors of Co-Fe@CBMA-MIPs for HSA, Bhb, OVA, and Lyz are 3.72, 6.09, 4.10, and 8.41, respectively. More significantly, Co-Fe@CBMA-MIPs could specifically recognize BSA from mixed proteins and actual samples and exhibit excellent repeated use stability. Based on the above advantages, the development of this research provides an effective means to improve the recognition specificity of molecularly imprinted polymers.

16.
Water Res ; 201: 117223, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34146760

RESUMO

Manganese contamination is ubiquitous in ground water. Water eutrophication also exaggerates manganese release and contamination in surface water. However, conventional manganese(II) removal process through sand filter is low-efficiency and long-term ripening. Manganese exceeding standard is still a bottleneck issue for drinking water plants. To provide a quick-setup and low-cost means, we invented an accelerated catalytic oxidation filtration process through porous zeolite filter with dynamically coating of manganese oxide nanocatalysts. In dynamic filtration process, the addition of chlorine less than redox stoichiometric consumption can efficiently remove dissolved manganese(II) from contaminated tap water, ground water and Songhua river water. Characterization results showed that a continuous manganese(III)/(IV) oxide nanosheet catalyst was dynamically in situ-growing and assembled into 3D porous superstructure in the reactive Zeolite@MnOx(s) filter. Active Mn(III) species on the edges of MnOx(s) nanosheets were dynamically generated and transferred into stable Mn(IV) species on the layer-structured surface. The cycling transformation of manganese(III)/(IV) species was responsible for the accelerated catalytic oxidation of dissolved manganese(II) by chlorine. Without process changes in drinking water plant, the porous Zeolite@MnOx(s) media could be feasibly integrated onto the existing sand filtration tanks for emergence handling of manganese(II) contamination. This novel reactive Zeolite@MnOx(s) filter with higher hydraulic conductivity provides a high-efficiency, scalable and low-cost technique for the manganese(II) removal from various of water environments.


Assuntos
Manganês , Zeolitas , Cloro , Compostos de Manganês , Oxirredução , Óxidos
17.
Brain Behav ; 11(7): e02186, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34096190

RESUMO

OBJECTIVES: Bipolar disorder (BD) is a common chronic mental illness. The circadian clock disorder shows a significant correlation with the pathogenesis, phenotype and recurrence of BD. We aim to evaluate non-invasive methods that can comprehensively assess the circadian rhythmicity in BD patients. METHODS: We non-invasively collected salivary samples and oral epithelial cells from recruited subjects. Then the levels of cortisol and melatonin in saliva were measured and the circadian clock gene expressions (PER2 and BMAL1) of epithelial cells were analyzed. Due to the disease characteristics of the manic patients who were difficult to cooperate with the protocol, only one patient at manic episode was recruited. Besides, 11 patients at the depressive episode, 15 healthy controls and four patients at recovery stage were recruited. RESULTS: Our results exhibited that the peak phase of cortisol level mainly manifested around 8:00 a.m., and the maximal melatonin level reached around 5:00 a.m. The phase of cortisol in patients with depression did not change significantly, but the level of cortisol decreased significantly, while the phase of melatonin level moved forward about 2.5 hr. Furthermore, the levels and phases of cortisol and melatonin in recovery patients tended to be similar to those of healthy controls. CONCLUSIONS: With detailed parameter analysis, the combined detection of melatonin and cortisol can better judge the biological clock disorder of bipolar patients. The circadian rhythms of patients at the recovery stage tend to be normal. The clock gene expression examination needs strict quality control and more investigations before being applied to assess human circadian rhythms.


Assuntos
Transtorno Bipolar , Melatonina , Ritmo Circadiano , Humanos , Hidrocortisona , Saliva
18.
Clinics (Sao Paulo) ; 76: e2081, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33978071

RESUMO

OBJECTIVES: Extracellular vesicle microRNAs (EV-miRNAs) have been demonstrated to be reliable candidate biomarkers for clinical applications. However, the clinical application potential of serum EV-miR-215-5p for gastric cancer (GC) remains poorly understood. The goal of our study was to determine the efficacy of serum EV-miR-215-5p in predicting the prognosis of GC. METHODS: Blood samples were collected from 118 patients with GC, 60 patients with benign gastric disease and BGD and 70 healthy controls. The relative levels of serum EV-miR-215-5p were measured using quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Compared to patients with BGD and normal controls, GC patients exhibited remarkably higher serum EV-miR-215-5p level, especially those with early tumor recurrence (ETR). Receiver operating characteristic (ROC) curve analysis showed that serum EV-miR-215-5p was able to distinguish GC patients from BGD patients or healthy controls and GC patients with ETR from those without ETR. In addition, increased serum EV-miR-215-5p levels were notably correlated with invasive depth, TNM stage, and lymph node metastasis. Moreover, serum EV-miR-215-5p levels were greatly decreased after surgical treatment, but increased at the time of ETR. Survival analysis showed that patients with higher serum EV-miR-215-5p had shorter survival. Furthermore, serum EV-miR-215-5p was an independent risk factor for GC. CONCLUSIONS: Serum EV-miR-215-5p might be a novel biomarker for predicting ETR and prognosis of GC.


Assuntos
Vesículas Extracelulares , MicroRNAs , Neoplasias Gástricas , Biomarcadores Tumorais , Humanos , Recidiva Local de Neoplasia , Prognóstico , Neoplasias Gástricas/genética
19.
Int J Pediatr Otorhinolaryngol ; 145: 110715, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33892339

RESUMO

BACKGROUND: Biallelic mutations in LOXHD1 have been identified as the cause of DFNB77 (deafness, autosomal recessive 77). It is a new progressive, severe-to-profound, and late-onset nonsyndromic sensorineural hearing loss (NSHL), and is highly heterogeneous genetically and phenotypically. This study aimed to provide an additional three cases of DFNB77. METHODS: We presented three unrelated children diagnosed with prelingual mild-to-severe NSHL, and their audiograms showed mild hearing loss at 250 Hz before downsloping to a moderate-to-severe degree. Trio whole-exome sequencing (WES) was conducted to identify the pathogenic variants. Additionally, we reviewed the literature to further analyze the relationships between the genotype and audiology phenotype of LOXHD1. RESULTS: Six novel possible pathogenic LOXHD1 variants were identified, including three missense, one nonsense, and two splicing variants. The literature review showed that 68.5% of patients with DFNB77 onset before five years old; Most variants (62%) were associated with a down-sloping audiogram of mild-to-moderate hearing loss at low frequencies (200Hz, 500Hz), particularly variants in the protein domain of PLAT 9. We found that compared with homozygous LOXHD1 variants, individuals with heterozygous compound variants had a significantly milder phenotype, especially individuals carrying one missense and one splicing or bi-allelic missense variants (P < 0.05). Audiometric analysis at different ages showed that the hearing loss degree was aggravated at all frequencies by increasing age. CONCLUSIONS: We report three children with prelingual NSHL carrying six novel LOXHD1 variants. Furthermore, our work indicates that DFNB77 may be milder than previously reported and recommends considering the genotype combination and mutation location of LOXHD1 and race-specificity in DFNB77 molecular diagnoses and management.


Assuntos
Proteínas de Transporte , Surdez , Perda Auditiva Neurossensorial , Perda Auditiva , Proteínas de Transporte/genética , Criança , Pré-Escolar , Estudos de Associação Genética , Perda Auditiva Neurossensorial/diagnóstico , Perda Auditiva Neurossensorial/genética , Humanos , Mutação , Linhagem , Fenótipo
20.
Phytopathology ; 2021 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-33900117

RESUMO

Extensive efforts have been devoted to discover new bio-fungicides of high efficiency for control of Fusarium oxysporum f. sp. cubense race 4 (Foc4), a catastrophic soil-borne phytopathogen causing banana fusarium wilt worldwide. In this paper, aureoverticillactam (YY3) was verified to possess a potent antifungal activity against Foc4 for the first time, with the EC50 values of 20.80 µg/ml against hyphal growth, and 12.62 µg/ml against spore germination. To provide insight into its action mechanism, the cellular ultrastructures of Foc4 was observed with YY3 treatment and the results revealed that YY3 led to cell wall thinning, mitochondrial deformities, apoptotic degradation of the subcellular fractions and entocyte leakage. Consistent with these variations, the enhanced permeability of cell membrane and mitochondrial membrane also occurred after YY3 treatment. At the enzymatic level, the activity of mitochondrial complex III, as well as the ATP synthase, was significantly suppressed by YY3 at a concentration above 12.50 µg/ml. Moreover, YY3 elevated the cytosolic Ca2+ level to promote the mitochondrial ROS production. The cell apoptosis also occurred as expected. At the transcriptome level, key genes involved in phosphatidylinositol signaling pathway were significantly impacted, with the expression level of Plc1 elevated by approximately 4 folds. The expression levels of two apoptotic genes, casA1 and casA2, were also significantly elevated by YY3. Of note, the activation of phospholipase C was observed by YY3 treatment in Foc4. These findings indicate that YY3 exerts its antifungal activity via activating the phospholipase C-calcium-dependent ROS signaling pathway, which makes it a great potential bio-fungicide.

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