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1.
Adv Mater ; : e2209768, 2023 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-36738144

RESUMO

Molecular solar thermal (MOST) materials, which can efficiently capture solar energy and release it as heat on demand, are promising candidates for future personal thermal management (PTM) applications, preferably in the form of fabrics. However, developing MOST fabrics with high energy storage capacity and stable working performance remains a significant challenge because of low energy density of the molecular materials and their leakage from the fabric. Here, we report an efficient and robust MOST fabric for PTM using azopyrazole-containing microcapsules with a deep-UV filter shell. The MOST fabric, which could co-harvest solar and thermal energy, achieved efficient photo-charging and photo-discharging (>90% photoconversion), a high energy density of 2.5 kJ m-2 , and long-term storage sustainability at month scale. Moreover, it could undergo multiple cycles of washing, rubbing, and recharging without significant loss of energy storage capacity. This MOST microcapsule strategy was easily used for the scalable production of a MOST fabric for solar thermal moxibustion. This achievement offers a promising route for the application of wearable MOST materials with high energy storage performance and robustness in PTM. This article is protected by copyright. All rights reserved.

2.
J Pharm Pharmacol ; 2023 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-36734364

RESUMO

OBJECTIVES: Ginsenoside Rg1 (Rg1) has been well-documented to be effective against ischemic/reperfusion (I/R) injury. However, whether it has therapeutic effect on delayed neuronal death is still unclear. The aim of this study is to investigate the effect of Rg1 on delayed neuronal death and elucidate its underlying mechanism. METHODS: Delayed neuronal death model was prepared by global cerebral ischemia-reperfusion in rats, Rg1 was intravenously administered once a day. Nissl and Fluoro Jade B staining were carried out to evaluate the effect of Rg1 on delayed neuronal death. Western blot and qPCR were used to investigate the levels of HBXIP and Survivin. HBXIP/Survivin complex was observed by co-immunoprecipitation. AAV-CMV-shRNA (HBXIP) was used to observe the role of HBXIP on delayed neuronal death improved by Rg1. KEY FINDINGS: Rg1 attenuated delayed neuronal death at the dose of 20 mg/kg, which also improved the mRNA and protein levels of HBXIP, as well as Survivin. Moreover, administration of Rg1 promoted the formation of HBXIP/Survivin complex, which contributed to the reduction of caspases signaling pathway. Knockdown of HBXIP abolished the alleviation of DND and inhibition of caspase cascade induced by Rg1. CONCLUSIONS: Rg1 alleviated delayed neuronal death by promoting anti-apoptosis effect by HBXIP/Survivin complex.

3.
Sci Adv ; 9(5): eadd6995, 2023 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-36724291

RESUMO

One of the major obstacles to treating pancreatic ductal adenocarcinoma (PDAC) is its immunoresistant microenvironment. The functional importance and molecular mechanisms of Schwann cells in PDAC remains largely elusive. We characterized the gene signature of tumor-associated nonmyelinating Schwann cells (TASc) in PDAC and indicated that the abundance of TASc was correlated with immune suppressive tumor microenvironment and the unfavorable outcome of patients with PDAC. Depletion of pancreatic-specific TASc promoted the tumorigenesis of PDAC tumors. TASc-expressed long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) was triggered by the tumor cell-produced interleukin-6. Mechanistically, PVT1 modulated RAF proto-oncogene serine/threonine protein kinase-mediated phosphorylation of tryptophan 2,3-dioxygenase in TASc, facilitating its enzymatic activities in catalysis of tryptophan to kynurenine. Depletion of TASc-expressed PVT1 suppressed PDAC tumor growth. Furthermore, depletion of TASc using a small-molecule inhibitor effectively sensitized PDAC to immunotherapy, signifying the important roles of TASc in PDAC immune resistance.


Assuntos
Carcinoma Ductal Pancreático , Neoplasias Pancreáticas , RNA Longo não Codificante , Humanos , Cinurenina/genética , Cinurenina/metabolismo , Proliferação de Células/genética , Linhagem Celular Tumoral , Neoplasias Pancreáticas/patologia , Carcinoma Ductal Pancreático/metabolismo , Regulação Neoplásica da Expressão Gênica , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Microambiente Tumoral/genética
4.
Zhongguo Zhong Yao Za Zhi ; 48(1): 96-104, 2023 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-36725262

RESUMO

By various chromatographic techniques and extensive spectroscopic methods, 17 abietane diterpenoids were isolated from the dichloromethane fraction of the 95% ethanol cold-soak extracts of the seeds of Pseudolarix amabilis, namely pseudoamaol A(1), 12α-hydroxyabietic acid(2), 12-methoxy-7,13-abietadien-18-oic acid(3), 13-hydroxy-8,11,13-podocarpatrien-18-oic acid(4), 15-hydroxy-7,13-abietadien-12-on-18-oic acid(5), 8(14)-podocarpen-13-on-18-oic acid(6), holophyllin K(7), metaglyptin B(8), 7α-hydroxydehydroabietinsaure-methylester(9), 7-oxodehydroabietic acid(10), 15-hydroxy-7-oxodehydroabietinsaure-methy-lester(11), 15-methoxydidehydroabietic acid(12), 7-oxo-15-hydroxy-dehydroabietic acid(13), 15-hydroxydehydroabietic acid(14), 8,11,13-abietatriene-15,18-diol(15), 8,11,13-abietatriene-15-hydroxy-18-succinic acid(16), and 7ß-hydroxydehydroabie-tic acid(17). Compound 1 was a new compound. The isolated compounds were evaluated for their antitumor activities(HepG2, SH-SY5Y, K562), and compounds 8 and 17 showed potential cytotoxic activity against K562 cells, with IC_(50) values of 26.77 and 37.35 µmol·L~(-1), respectively.


Assuntos
Antineoplásicos , Diterpenos , Neuroblastoma , Humanos , Estrutura Molecular , Diterpenos/farmacologia , Diterpenos/química
5.
Appl Microbiol Biotechnol ; 107(4): 1477-1490, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36692514

RESUMO

Nitrogen (N) and phosphorus (P) are two major pollutants present in aquaculture wastewater, and their concentrations often do not meet discharge standards. In the present study, the N and P removal efficiency of nutrient-deficient cells (S group) was significantly higher than that of photoautotrophic cells (P group) and heterotrophic cells (H group). After incubation with wastewater, the N and P content of S group cells was significantly increased and reached a level similar to that of the P group and H group cells after 6 days of treatment. Additionally, in the S group cells, the content of total fatty acids (TFAs), which can be used to supply energy and organic carbon for N and P absorption, significantly decreased. In addition, the protein and nucleic acid contents of the S group cells also significantly increased, which revealed the biosynthetic flow of assimilated N and P. Comparative transcriptome analysis showed that compared with the P group and H group, the N metabolism, ribosome, RNA polymerase, and fatty acid degradation pathways were significantly upregulated in the S group cells, and the fatty acid biosynthesis pathway was significantly downregulated, which was in agreement with the biochemical results. In summary, our study showed that N- and P-starved heterotrophic cells are ideal for use in wastewater N and P removal processes. Keypoints • The N and P removal efficiencies of the S group were higher than P and H groups • Fatty acids were degraded to supply energy and carbon for N and P absorption • N metabolism and fatty acid degradation pathways were upregulated in the S group.

6.
Sci Total Environ ; 865: 161245, 2023 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-36587661

RESUMO

The phyllosphere is a fluctuant micro-environment habitat that harbors diverse microbial communities that have the potential to influence plant growth through their effect on host fitness. However, we know little about the driving factors of phyllosphere microbial functional traits, e.g., genes related to nutrient cycling and microbial community structure under anthropic disturbance. Here, we characterized phyllosphere microbial communities and the abundance of genes related to nutrient cycling from diverse plant species between urban and natural habitats. We measured leaf functional traits to investigate the potential drivers of the phyllosphere microbial profile. Results indicated that phyllosphere microbial communities differed significantly between urban and natural habitats, and that this variation was dependent upon plant species. Host plant species had a greater influence on the abundance of genes involved in nutrient cycling in the phyllosphere than habitat. In addition, phyllosphere microbial diversity and functional gene abundance were significantly correlated. Furthermore, host leaf functional traits (e.g., specific leaf area and nutrient content) were potential driving factors of both phyllosphere microbial community structure and the abundance of genes involved in nutrient cycling. These findings contribute to a better understanding of the phyllosphere microbiome and its biotic and abiotic controlling factors, which improves our understanding of plant-microbe interactions and their ecosystem functions under anthropic disturbance.


Assuntos
Microbiota , Plantas , Nutrientes , Folhas de Planta/química
7.
Artigo em Inglês | MEDLINE | ID: mdl-36690570

RESUMO

Transition metal (TM) sulfides are promising catalysts for water splitting in alkaline media due to their high intrinsic activities and similar TM-S electronic structure with hydrogenase. In this work, the nanoporous FeCoNbS electrocatalyst with nanosheet morphology is synthesized through dealloying AlFeCoNb alloy followed by the steam sulphurization. The introduction of S element improves the electronic structure, further increases the active sites, regulates the mass transfer and enhances the intrinsic activity. The Nb introduction improves the electron transfer ability of the catalyst. The synergistic effect of Fe, Co and Nb improves the intrinsic activity of the active site. The FeCoNbS catalyst exhibits good catalytic performance for both hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) in alkaline solution. The overpotentials at 10 mA cm-2 of HER and OER are 83 and 241 mV, respectively. The Tafel slopes of HER and OER are 101.2 and 35.5 mV dec-1, respectively. The FeCoNbS can serve as overall water splitting electrode with the decomposition voltage of 1.61 V at 10 mA cm-2.

8.
Animals (Basel) ; 13(2)2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36670855

RESUMO

BACKGROUND: Reptiles are asymptomatic carriers of Salmonella spp. Reptile-associated Salmonella infections have been noticed as a significant contributor to overall human salmonellosis. However, it remains unclear regarding the prevalence of reptile-associated Salmonella in China. METHODS: Fecal and gastrointestinal mucosal samples were taken from 104 snakes, 21 lizards, and 52 chelonians and cultured on selective medium. The positive clones were validated and annotated by biochemical screening and multiplex PCR verification. In addition, the antibiotic resistance of identified Salmonella isolates was detected and followed by cytotoxic activity detection on human colon cells via co-culturation. RESULTS: The overall prevalence of Salmonella in reptiles was 25.99%, with rates of 30.77%, 47.62%, and 7.69% in snakes, lizards, and chelonians, respectively. Further, all isolates showed variable drug-resistant activity to 18 antibiotics, of which 14 strains (30.43%) were resistant to more than eight kinds of antibiotics. More than half of isolated Salmonella strains were more toxic to host cells than the standard strain, SL1344. Whole genome sequencing (WGS) results showed that all lizard-associated strains belong to 4 serovar types, and 7 of them fall into the highly pathogenic serovars "Carmel" and "Pomona." CONCLUSIONS: Our results highlight the potential threat of zoonotic salmonellosis from captive reptiles in the Beijing area of China.

9.
Transp Res Part A Policy Pract ; 169: 103586, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36685313

RESUMO

The spread of COVID-19 results in a significant drop in traffic levels worldwide. Quantifying the impact of multiple COVID-19 outbreaks on traffic systems is critical to developing differentiated policies in the future. This paper proposes a novel COVID-19 multiple outbreak analysis method (NCMOA), dividing the impact scope and degree under multiple COVID-19 disturbances, and using the recovery rate and accumulated loss to quantify the impacts on air passenger flow. A case study based on Chinese national air traffic flow is executed, and the recovery patterns and the differentiated disturbances are analyzed. Results show that air passenger flow recovers with a similar pattern after the first outbreak, and subsequent outbreaks cause local effects and cannot affect the overall recovery pattern. Further, the heterogeneous influence factors and trends on the epi-centers (EC) and the nation are analyzed. In addition, the methods and results of this paper quantify the impact of COVID-19 on air passenger flow at a more detailed level under multiple disturbances. They could provide a basis for differentiated policy formulation of airlines and government in the future.

11.
Int Immunopharmacol ; 116: 109781, 2023 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-36720195

RESUMO

Chemokines, as small molecular proteins, play a crucial role in the immune and inflammatory responses after stroke. A large amount of evidence showed chemokines and their receptors were increasingly recognized as potential targets for stroke treatment, which were involved in the processing of neovascularization, neurogenesis, and neural network reconstruction. In this review, we summarized the characteristics of chemokine alterations throughout the post-stroke nerve repair phase to gain insight into the pathological mechanisms of chemokines and find effective therapeutic targets for stroke.

12.
Mater Horiz ; 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36637149

RESUMO

As a promising environment-friendly alternative to current vapor-compression refrigeration, solid-state refrigeration based on the barocaloric effect has been attracting worldwide attention. Generally, both phases in which a barocaloric effect occurs are present at ambient pressure. Here, instead, we demonstrate that KPF6 exhibits a colossal barocaloric effect due to the creation of a high-pressure rhombohedral phase. The phase diagram is constructed based on pressure-dependent calorimetric, Raman scattering, and neutron diffraction measurements. The present study is expected to provide an alternative routine to colossal barocaloric effects through the creation of a high-pressure phase.

13.
Int Immunopharmacol ; 115: 109684, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36630752

RESUMO

OBJECTIVES: Osteosarcoma is highly aggressive and prone to metastasis, with a poor prognosis. Increasing evidence identified anoikis has a critical effect in tumor metastasis and invasion. However, the prognostic value of anoikis-related genes (ANRGs) in osteosarcoma and their role in the immune landscape of osteosarcoma remain unclear. METHODS: The RNA sequencing and clinical data of patients with osteosarcoma were extracted from the TARGET and GEO databases, and ANRGs were identified from the GeneCards database. Unsupervised clustering analysis was employed to identify anoikis-related patterns. The ESTIMATE, TIMER and ssGSEA algorithms were used to assess the immune microenvironment of different subtypes. A prognostic signature based on the identified ANRGs was constructed via univariate, LASSO and multivariate Cox regression analyses. KEGG, GO and GSEA were used for functional enrichment of genes associated with different risk subtypes. qPCR, WB and IHC were used to validate the expression of candidate genes. RESULTS: Two anoikis-related patterns with distinct clinical features and immune statuses were identified based on prognosis-related ANRGs. Cluster 2 had more active immunogenicity and a better prognosis than Cluster 1. Subsequently, we developed and validated an anoikis prognostic signature demonstrating excellent predictive ability for the prognosis of osteosarcoma. Anoikis risk score was positively associated with osteosarcoma metastasis and was identified as an independent prognostic marker. Additionally, a nomogram was established to predict the 3- and 5-year survival probability of patients with osteosarcoma. Functional enrichment analysis revealed that immune dysregulation was correlated with poor prognosis. Besides, patients in the low-risk group had higher infiltration levels of immune cells and more active immune function than patients in the high-risk group. Drug sensitivity analysis revealed several chemotherapeutic agents for the treatment of different subtypes of osteosarcoma. CONCLUSION: Our study demonstrated the role of ANRGs in osteosarcoma progression, providing insights into clinical decision making in osteosarcoma.

14.
Adv Healthc Mater ; : e2202663, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36653312

RESUMO

Ferroptosis, characterized by the accumulation of reactive oxygen species and lipid peroxides, has emerged as an attractive strategy to reverse drug resistance. Of particular interest is the ferroptosis-apoptosis combination therapy for cancer treatment. Herein, a nanoplatform is reported for effective co-delivery of the anticancer drug sorafenib (S) and the ferroptosis inducer hemin (H), toward synergistic ferroptosis-apoptosis therapy of advanced hepatocellular carcinoma (HCC) as a proof-of-concept study. Liposome is an excellent delivery system; however, it is not sufficiently responsive to the acidic tumor microenvironment (TME) for tumor-targeted drug delivery. The pH-sensitive vesicles are therefore developed (SH-AD-L) by incorporating amphiphilic dendrimers (AD) into liposomes for controlled and pH-stimulated release of sorafenib and hemin in the acidic TME, thanks to the protonation of numerous amine functionalities in AD. Importantly, SH-AD-L not only blocked glutathione synthesis to disrupt the antioxidant system, but also increased intracellular Fe2+ and •OH concentrations to amplify oxidative stress, both of which contribute to enhanced ferroptosis. Remarkably, high levels of •OH also augmented sorafenib-mediated apoptosis in tumor cells. This study demonstrates the efficacy of ferroptosis-apoptosis combination therapy, as well as the promise of the AD-doped TME-responsive vesicles for drug delivery in combination therapy to treat advanced HCC.

15.
PLoS One ; 18(1): e0280905, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36706149

RESUMO

Matrine, an alkaloid derived from herbal medicine, has a wide range of biological activities, including antibacterial. Matrine was toxic to multiple cells at high concentrations. Bovine mammary epithelial cells (MAC-T) could be used as model cells for cow breast. Matrine was a feasible option to replace antibiotics in the prevention or treatment of mastitis against the background of prohibiting antibiotics, but the safe concentration of matrine on MAC-T cells and the mechanism of action for matrine at different concentrations were still unclear. In this study, different concentrations of matrine (0.5, 1, 1.5, 2, 2.5 and 3 mg/mL) were used to treat MAC-T cells for various time periods (4, 8, 12, 16 and 24 h) and measure their lactic dehydrogenase (LDH). And then the optimal doses (2 mg/mL) were chosen to detect the apoptosis at various time periods by flow cytometry and transcriptome analysis was performed between the control and 2 mg/mL matrine-treated MAC-T cells for 8 hours. The results showed that matrine was not cytotoxic at 0.5 mg/mL, but it was cytotoxic at 1~3 mg/mL. In addition, matrine induced apoptosis in MAC-T cells at 2 mg/mL and the proportion of apoptosis cells increases with time by flow cytometry. RNA-seq analysis identified 1645 DEGs, 676 of which were expressed up-regulated and 969 were expressed down-regulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated the following pathways were linked to matrine-induced toxicity and apoptosis, including cytokine-cytokine receptor interaction pathway, viral protein interaction with cytokine and cytokine receptor, P53 and PPAR pathway. We found 7 DEGs associated with matrine toxicity and apoptosis. This study would provide a basis for the safety of matrine in the prevention or treatment of mastitis.


Assuntos
Antineoplásicos , Transcriptoma , Feminino , Animais , Bovinos , Linfócitos T , Apoptose , Antineoplásicos/farmacologia , Citocinas/farmacologia , Quinolizinas/farmacologia , Quinolizinas/uso terapêutico
16.
Int J Disaster Risk Reduct ; 85: 103517, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36593901

RESUMO

Since the outbreak of COVID-19 in China in late 2019, government administrators have implemented traffic restriction policies to prevent the spread of COVID-19. However, highway traffic volumes obtained from ETC data in some provinces did not return to the levels of previous years after the end of the traffic restriction policy, suggesting that traffic restriction policy may have long-term effects. This paper proposed a method that analyzes traffic restriction policies' long-term and short-term impact on highway traffic volume under COVID-19. This method first analyzes the long-term and short-term impacts of traffic restriction policies on the highway traffic volume using the Prophet model combined with the concept of traffic volume loss. It further investigates the relationship between COVID-19 cases and the long-term and short-term impacts of the traffic restriction policy using Granger causality and the impulse response function of the Bayesian vector autoregressive (BVAR) model. The results showed that during the COVID-19 pandemic, highway traffic in Zhejiang Province decreased by about 95.5%, and the short-term impact of COVID-19 cases was most pronounced on the second day. However, the long-term effects were relatively small when the traffic restriction policy ended and was verified by data from other provinces. These results will provide decision support for traffic management and provide recommendations for future traffic impact assessments in the event of similar epidemics.

17.
Zhongguo Gu Shang ; 36(1): 43-7, 2023 Jan 25.
Artigo em Chinês | MEDLINE | ID: mdl-36653005

RESUMO

OBJECTIVE: To investigate the clinical efficacy and safety of percutaneous foraminal endoscopy in the treatment of lumbar lateral recess stenosis in elderly. METHODS: The clinical data of 31 elderly patients with lumbar lateral recess stenosis treated by percutaneous foraminal endoscopic decompression from March 2018 to August 2019 were retrospectively analyzed. Including 16 males and 15 females, aged from 65 to 81 years with an average of (71.13±5.20) years, the course of disease ranged from 3 months to 7 years with an average of (14.36±6.52) months. Visual analogue scale (VAS) and Oswestry disability index (ODI) were used to assess clinical symptom and functional status before operation and 1, 6, 12 months after operation. At the final follow-up, the modified Macnab standard was used to evaluate clinical efficacy. RESULTS: All patients were completed the operation successfully. The operation time was from 75 to 120 min with an average of (97.84±11.22 ) min. All 31 patients were followed up from 12 to 28 months with an average of (17.29±5.56) months. Postoperative lumbago-leg pain VAS and ODI were significantly improved at 1, 6, and 12 months(P<0.01). At the final follow-up, according to the modified Macnab standard to evaluate the effect, 23 got excellent results, 5 good, 3 fair. One patient had severe adhesions between peripheral tissues and nerve root, and postoperative sensory abnormalities in the lower extremities were treated conservatively with traditional Chinese medicine and neurotrophic drugs, which recovered at 2 weeks after surgery. No complications such as nerve root injury and infection occurred. CONCLUSION: The intervertebral foraminal endoscopy technique, which is performed under local anesthesia for a short period of operation, ensures adequate decompression while minimizing complications, and is a safe and effective surgical procedure for elderly patients with lumbar lateral recess stenosis.


Assuntos
Estenose Espinal , Masculino , Feminino , Humanos , Idoso , Lactente , Constrição Patológica/cirurgia , Estenose Espinal/cirurgia , Descompressão Cirúrgica/métodos , Estudos Retrospectivos , Vértebras Lombares/cirurgia , Endoscopia/métodos , Resultado do Tratamento
18.
Nat Commun ; 14(1): 22, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36596772

RESUMO

Constructing high-quality haplotype-resolved genome assemblies has substantially improved the ability to detect and characterize genetic variants. A targeted approach providing readily access to the rich information from haplotype-resolved genome assemblies will be appealing to groups of basic researchers and medical scientists focused on specific genomic regions. Here, using the 4.5 megabase, notoriously difficult-to-assemble major histocompatibility complex (MHC) region as an example, we demonstrated an approach to construct haplotype-resolved assembly of the targeted genomic region with the CRISPR-based enrichment. Compared to the results from haplotype-resolved genome assembly, our targeted approach achieved comparable completeness and accuracy with reduced computing complexity, sequencing cost, as well as the amount of starting materials. Moreover, using the targeted assembled personal MHC haplotypes as the reference both improves the quantification accuracy for sequencing data and enables allele-specific functional genomics analyses of the MHC region. Given its highly efficient use of resources, our approach can greatly facilitate population genetic studies of targeted regions, and may pave a new way to elucidate the molecular mechanisms in disease etiology.


Assuntos
Genoma Humano , Genômica , Humanos , Haplótipos/genética , Análise de Sequência de DNA/métodos , Genoma Humano/genética , Genômica/métodos , Complexo Principal de Histocompatibilidade/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos
19.
Med Biol Eng Comput ; 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36598674

RESUMO

Dynamic contrast-enhanced MRI (DCE-MRI) is routinely included in the prostate MRI protocol for a long time; its role has been questioned. It provides rich spatial and temporal information. However, the contained information cannot be fully extracted in radiologists' visual evaluation. More sophisticated computer algorithms are needed to extract the higher-order information. The purpose of this study was to apply a new deep learning algorithm, the bi-directional convolutional long short-term memory (CLSTM) network, and the radiomics analysis for differential diagnosis of PCa and benign prostatic hyperplasia (BPH). To systematically investigate the optimal amount of peritumoral tissue for improving diagnosis, a total of 9 ROIs were delineated by using 3 different methods. The results showed that bi-directional CLSTM with ± 20% region growing peritumoral ROI achieved the mean AUC of 0.89, better than the mean AUC of 0.84 by using the tumor alone without any peritumoral tissue (p = 0.25, not significant). For all 9 ROIs, deep learning had higher AUC than radiomics, but only reaching the significant difference for ± 20% region growing peritumoral ROI (0.89 vs. 0.79, p = 0.04). In conclusion, the kinetic information extracted from DCE-MRI using bi-directional CLSTM may provide helpful supplementary information for diagnosis of PCa.

20.
Comput Methods Programs Biomed ; 228: 107249, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36423486

RESUMO

BACKGROUND AND OBJECTIVE: The Chinese medical question answer matching (cMedQAM) task is the essential branch of the medical question answering system. Its goal is to accurately choose the correct response from a pool of candidate answers. The relatively effective methods are deep neural network-based and attention-based to obtain rich question-and-answer representations. However, those methods overlook the crucial characteristics of Chinese characters: glyphs and pinyin. Furthermore, they lose the local semantic information of the phrase by generating attention information using only relevant medical keywords. To address this challenge, we propose the multi-scale context-aware interaction approach based on multi-granularity embedding (MAGE) in this paper. METHODS: We adapted ChineseBERT, which integrates Chinese characters glyphs and pinyin information into the language model and fine-tunes the medical corpus. It solves the common phenomenon of homonyms in Chinese. Moreover, we proposed a context-aware interactive module to correctly align question and answer sequences and infer semantic relationships. Finally, we utilized the multi-view fusion method to combine local semantic features and attention representation. RESULTS: We conducted validation experiments on the three publicly available datasets, namely cMedQA V1.0, cMedQA V2.0, and cEpilepsyQA. The proposed multi-scale context-aware interaction approach based on the multi-granularity embedding method is validated by top-1 accuracy. On cMedQA V1.0, cMedQA V2.0, and cEpilepsyQA, the top-1 accuracy on the test dataset was improved by 74.1%, 82.7%, and 60.9%, respectively. Experimental results on the three datasets demonstrate that our MAGE achieves superior performance over state-of-the-art methods for the Chinese medical question answer matching tasks. CONCLUSIONS: The experiment results indicate that the proposed model can improve the accuracy of the Chinese medical question answer matching task. Therefore, it may be considered a potential intelligent assistant tool for the future Chinese medical answer question system.


Assuntos
Idioma , Humanos
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