Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.485
Filtrar
1.
Molecules ; 26(5)2021 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-33806510

RESUMO

It has been reported that monoamine neurotransmitters can be produced by gut microbiota, and that several related metabolites of amino acids in these pathways are associated with nervous system (NVS) diseases. Herein, we focused on three pathways, namely, phenylalanine (Phe), tryptophan (Trp), and glutamic acid (Glu), and established an underivatized liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of nineteen monoamine neurotransmitters and related metabolites in the gut microbiota. The neurotransmitters and related metabolites included Phe, tyrosine (Tyr), l-dopa (Dopa), dopamine (DA), 3-methoxytyramine, Trp, hydroxytryptophan, 5-hydroxytryptamine (5-HT), 5-hydroxyindole-3-acetic acid (5-HIAA), kynurenine (KN), kynurenic acid (KYNA), melatonin, tryptamine (TA), indole-3-lactic acid (ILA), indole-3-acetic acid (IAA), indolyl-3-propionic acid (IPA), Glu, gamma-aminobutyric acid (GABA), and acetylcholine (Ach). A fluoro-phenyl bonded column was used for separation, and the mobile phase consisted of methanol:acetonitrile (1:1) and water, with 0.2% formic acid in both phases. The compounds exhibited symmetric peak shapes and sufficient sensitivity under a total analysis time of 8.5 min. The method was fully validated with acceptable linearity, accuracy, precision, matrix effect, extraction recovery, and stability. The results showed that neurotransmitters, such as Dopa, DA, 5-HT, GABA, and Ach, were present in the gut microbiota. The metabolic pathway of Trp was disordered under depression, with lower levels of 5-HT, 5-HIAA, KN, KYNA, TA, ILA, IAA, IPA, and Glu, and a higher ratio of KYNA/KN. In addition, some first-line NVS drugs, such as sertraline, imipramine, and chlorpromazine, showed regulatory potential on these pathways in the gut microbiota.

2.
Dev Sci ; 2021 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-33817920

RESUMO

To explore the neural substrates of executive function (EF), we conducted an activation likelihood estimation meta-analysis of 408 functional magnetic resonance imaging studies (9639 participants, 7587 activation foci, 518 experimental contrasts) covering three fundamental EF subcomponents: inhibition, switching, and working memory. Our results found that activation common to all three EF subcomponents converged in the multiple-demand network across adolescence and adulthood. The function of EF with the multiple-demand network involved, especially for the prefrontal cortex and the parietal regions, could not be mature until adulthood. In adolescents, only working memory could be separable from common EF, whereas in adults, the three EF subcomponents could be separable from common EF. However, findings of switching in adolescents should be treated with substantial caution and may be exploratory due to limited data available on switching tasks. For task materials, inhibition and working memory showed both domain generality and domain specificity, undergirded by the multiple-demand network, as well as different brain regions in response to verbal and nonverbal task materials, respectively. In contrast, switching showed only domain generality with no activation specialized for either verbal or nonverbal task materials. These findings, taken together, support and contribute to the unitary-diverse nature of EF such that EF should be interpreted in an integrative model that relies on the integration of the EF construct, development, and task materials.

3.
Genes (Basel) ; 12(4)2021 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-33801713

RESUMO

The three structurally related orphan G protein-coupled receptors, GRP3, GPR6, and GPR12, are reported to be constitutively active and likely involved in the regulation of many physiological/pathological processes, such as neuronal outgrowth and oocyte meiotic arrest in mammals. However, the information regarding these orphan receptors in nonmammalian vertebrates is extremely limited. Here, we reported the structure, constitutive activity, and tissue expression of these receptors in two representative avian models: chickens and ducks. The cloned duck GPR3 and duck/chicken GPR6 and GPR12 are intron-less and encode receptors that show high amino acid (a.a.) sequence identities (66-88%) with their respective mammalian orthologs. Interestingly, a novel GPR12-like receptor (named GPR12L) sharing 66% a.a. identity to that in vertebrates was reported in the present study. Using dual-luciferase reporter assay and Western blot, we demonstrated that GPR3, GPR6, GPR12, and GPR12L are constitutively active and capable of stimulating the cAMP/PKA signaling pathway without ligand stimulation in birds (and zebrafish), indicating their conserved signaling property across vertebrates. RNA-seq data/qRT-PCR assays revealed that GPR6 and GPR12L expression is mainly restricted to the chicken brain, while GPR12 is highly expressed in chicken ovarian granulosa cells (GCs) and oocytes of 6 mm growing follicles and its expression in cultured GCs is upregulated by progesterone. Taken together, our data reveal the structure, function, and expression of GPR3, GPR6, GPR12, and GPR12L in birds, thus providing the first piece of evidence that GPR12 expression is upregulated by gonadal steroid (i.e., progesterone) in vertebrates.

4.
Artigo em Inglês | MEDLINE | ID: mdl-33835918

RESUMO

Transductive zero-shot learning (TZSL) extends conventional ZSL by leveraging (unlabeled) unseen images for model training. A typical method for ZSL involves learning embedding weights from the feature space to the semantic space. However, the learned weights in most existing methods are dominated by seen images, and can thus not be adapted to unseen images very well. In this paper, to align the (embedding) weights for better knowledge transfer between seen/unseen classes, we propose the virtual mainstay alignment network (VMAN), which is tailored for the transductive ZSL task. Specifically, VMAN is casted as a tied encoder-decoder net, thus only one linear mapping weights need to be learned. To explicitly learn the weights in VMAN, for the first time in ZSL, we propose to generate virtual mainstay (VM) samples for each seen class, which serve as new training data and can prevent the weights from being shifted to seen images, to some extent. Moreover, a weighted reconstruction scheme is proposed and incorporated into the model training phase, in both the semantic/feature spaces. In this way, the manifold relationships of the VM samples are well preserved. To further align the weights to adapt to more unseen images, a novel instance-category matching regularization is proposed for model re-training. VMAN is thus modeled as a nested minimization problem and is solved by a Taylor approximate optimization paradigm. In comprehensive evaluations on four benchmark datasets, VMAN achieves superior performances under the (Generalized) TZSL setting.

5.
Biomaterials ; 272: 120770, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33798957

RESUMO

Three-dimensional in vitro tumor models provide more physiologically relevant responses to drugs than 2D models, but the lack of proper evaluation indices and the laborious quantitation of tumor behavior in 3D have limited the use of 3D tumor models in large-scale preclinical drug screening. Here we propose two indices of 3D tumor invasiveness-the excess perimeter index (EPI) and the multiscale entropy index (MSEI)-and combine these indices with a new convolutional neural network-based algorithm for tumor spheroid boundary detection. This new algorithm for 3D tumor boundary detection and invasiveness analysis is more accurate than any other existing algorithms. We apply this spheroid monitoring and AI-based recognition technique ("SMART") to evaluating the invasiveness of tumor spheroids grown from tumor cell lines and from primary tumor cells in 3D culture.

6.
J Colloid Interface Sci ; 594: 1-8, 2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33744729

RESUMO

This study aims to prepare an organic-inorganic reticular polymer electrolyte. Isocyanate acts as a bridge that connects fumed silica and PEO molecular chains. The PEO-TDI-SiO2 solid polymer electrolytes developed can significantly have improved ionic conductivity of 0.12 mS cm-1 at ambient temperature. This is because the TDI-SiO2 nanoparticles inhibits polymer crystallization which provides more continuous Li-ion transport pathways. Tests at 60 °C indicate that the cross-linked structure of covalent TSI bonded to PEO effectively enlarges the electrochemical window of the polymer electrolyte to 5.6 V. Also, the PTSI electrolyte membrane has a higher Li+ transference number of 0.33 compared to the PEO-LiTFSI electrolytes. It is worth noting that the assembled LiFePO4|PTSI8%|Li cells deliver outstanding rate performance and stable cycling performance. All these considerable merits of PTSI membrane demonstrate that PTSI is a promising candidate that can be used as solid polymer electrolytes for the next-generation Li-ion batteries.

7.
Theranostics ; 11(9): 4155-4170, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33754054

RESUMO

Background: Anti-PD-1-based immunotherapy has emerged as a promising therapy for several cancers. However, it only benefits a small subset of colorectal cancer (CRC) patients. Mounting data supports the pivotal role of gut microbiota in shaping immune system. Pectin, a widely consumed soluble fiber, has been reported to ameliorate the imbalance of gut microbiota. Therefore, we aimed to explore the effect and the underlying mechanisms of pectin in improving anti-PD-1 mAb efficacy. Methods: The C57BL/6 mice were treated with a broad-spectrum antibiotic (ATB) cocktail to depleted endogenous gut microbiota and subsequently humanized with feces from healthy controls or newly diagnosed CRC patients. The antitumor efficacies of anti-PD-1 mAb combined with or without pectin were assessed using these mice. Flow cytometry and immunohistochemistry (IHC) were conducted to investigate the tumor immune microenvironment after treatment. The gut microbiota profiles and short-chain fatty acids (SCFAs) levels were determined by 16S ribosomal RNA (16S rRNA) gene sequencing and gas chromatography-mass spectrometry (GC-MS), respectively. The effect of gut microbiota on anti-PD-1 mAb efficacy after pectin supplement was further tested by fecal microbiota transplantation (FMT). Results: The anti-PD-1 mAb efficacy was largely impaired in the mice humanized with feces from newly diagnosed CRC patients compared to those from healthy controls. However, pectin significantly enhanced the anti-PD-1 mAb efficacy in the tumor-bearing mice humanized with CRC patient gut microbiota. Flow cytometry and IHC analysis revealed increased T cell infiltration and activation in the tumor microenvironment of mice treated with anti-PD-1 mAb plus pectin. In vivo depletion of CD8+ T cells diminished the anti-tumor effect of anti-PD-1 mAb combined with pectin. 16S rRNA gene sequencing showed that pectin significantly increased gut microbial diversity and beneficially regulated microbial composition. In addition, we identified unique bacterial modules that were significantly enriched in the anti-PD-1 mAb + pectin group, which composed of butyrate-producing bacteria indicative of good response to immunotherapy. Meanwhile, GC-MS showed that pectin altered the level of SCFA butyrate. Furthermore, butyrate, a main product of dietary fiber in gut microbial fermentation, was found to be sufficient to promote T cells infiltration and thus enhance the efficacy of anti-PD-1 mAb. In addition, FMT demonstrated the effects of pectin were dependent on gut microbiota. Importantly, the beneficial effects of pectin were confirmed in the mice humanized with gut microbiota from patient with resistance to anti-PD-1 mAb. Conclusion: Pectin facilitated the anti-PD-1 mAb efficacy in CRC via regulating the T cell infiltration in the tumor microenvironment, which was potentially mediated by the metabolite butyrate.

8.
Brief Bioinform ; 22(2): 1297-1308, 2021 03 22.
Artigo em Inglês | MEDLINE | ID: mdl-33757279

RESUMO

The life-threatening coronaviruses MERS-CoV, SARS-CoV-1 and SARS-CoV-2 (SARS-CoV-1/2) have caused and will continue to cause enormous morbidity and mortality to humans. Virus-encoded noncoding RNAs are poorly understood in coronaviruses. Data mining of viral-infection-related RNA-sequencing data has resulted in the identification of 28 754, 720 and 3437 circRNAs encoded by MERS-CoV, SARS-CoV-1 and SARS-CoV-2, respectively. MERS-CoV exhibits much more prominent ability to encode circRNAs in all genomic regions than those of SARS-CoV-1/2. Viral circRNAs typically exhibit low expression levels. Moreover, majority of the viral circRNAs exhibit expressions only in the late stage of viral infection. Analysis of the competitive interactions of viral circRNAs, human miRNAs and mRNAs in MERS-CoV infections reveals that viral circRNAs up-regulated genes related to mRNA splicing and processing in the early stage of viral infection, and regulated genes involved in diverse functions including cancer, metabolism, autophagy, viral infection in the late stage of viral infection. Similar analysis in SARS-CoV-2 infections reveals that its viral circRNAs down-regulated genes associated with metabolic processes of cholesterol, alcohol, fatty acid and up-regulated genes associated with cellular responses to oxidative stress in the late stage of viral infection. A few genes regulated by viral circRNAs from both MERS-CoV and SARS-CoV-2 were enriched in several biological processes such as response to reactive oxygen and centrosome localization. This study provides the first glimpse into viral circRNAs in three deadly coronaviruses and would serve as a valuable resource for further studies of circRNAs in coronaviruses.

9.
Med Image Anal ; 70: 102030, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33752167

RESUMO

Investigation of image reconstruction from data collected over a limited-angular range in X-ray CT remains a topic of active research because it may yield insight into the development of imaging workflow of practical significance. This reconstruction problem is well-known to be challenging, however, because it is highly ill-conditioned. In the work, we investigate optimization-based image reconstruction from data acquired over a limited-angular range that is considerably smaller than the angular range in short-scan CT. We first formulate the reconstruction problem as a convex optimization program with directional total-variation (TV) constraints applied to the image, and then develop an iterative algorithm, referred to as the directional-TV (DTV) algorithm for image reconstruction through solving the optimization program. We use the DTV algorithm to reconstruct images from data collected over a variety of limited-angular ranges for breast and bar phantoms of clinical- and industrial-application relevance. The study demonstrates that the DTV algorithm accurately recovers the phantoms from data generated over a significantly reduced angular range, and that it considerably diminishes artifacts observed otherwise in reconstructions of existing algorithms. We have also obtained empirical conditions on minimal-angular ranges sufficient for numerically accurate image reconstruction with the DTV algorithm.

10.
Eur J Med Chem ; 217: 113314, 2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33765606

RESUMO

Polo-like kinases (PLKs) play important roles in regulating multiple aspects of cell cycle and cell proliferation. In many cancer types, PLK family members are often dysregulated, which can lead to uncontrolled cell proliferation and aberrant cell division and has been shown to associate with poor prognosis of cancers. The key roles of PLK kinases in cancers lead to an enhanced interest in them as promising targets for anticancer drug development. In consideration of PLK inhibitors and some other anticancer agents, such as BRD4, EEF2K and Aurora inhibitors, exert synergy effects in cancer cells, dual-targeting of PLK and other cancer-related targets is regarded as an rational and potent strategy to enhance the effectiveness of single-targeting therapy for cancer treatment. This review introduces the PLK family members at first and then focuses on the recent advances of single-target PLK inhibitors and summarizes the corresponding SARs of them. Moreover, we discuss the synergisms between PLK and other anti-tumor targets, and sum up the current dual-target agents based on them.

11.
Org Lett ; 23(7): 2726-2730, 2021 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-33760616

RESUMO

We report a sequential catalytic asymmetric transfer hydrogenation-carboxylative cyclization for the facile construction of chiral 4-fluoroalkyl 2-oxazolidinones with high enantioselectivity. The resulting 2-oxazolidinones can be easily elaborated to synthetic useful chiral ß-fluoroalkyl ß-amino alcohols. This research also represents a rare example of catalytic asymmetric sequential reactions using CO2 as a C1 synthon as well as carboxylative cyclization of α-fluoroalkyl propargylamines.

12.
IEEE Trans Cybern ; PP2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33750731

RESUMO

In convolutional neural networks (CNNs), generating noise for the intermediate feature is a hot research topic in improving generalization. The existing methods usually regularize the CNNs by producing multiplicative noise (regularization weights), called multiplicative regularization (Multi-Reg). However, Multi-Reg methods usually focus on improving generalization but fail to jointly consider optimization, leading to unstable learning with slow convergence. Moreover, Multi-Reg methods are not flexible enough since the regularization weights are generated from a definite manual-design distribution. Besides, most popular methods are not universal enough, because these methods are only designed for the residual networks. In this article, we, for the first time, experimentally and theoretically explore the nature of generating noise in the intermediate features for popular CNNs. We demonstrate that injecting noise in the feature space can be transformed to generating noise in the input space, and these methods regularize the networks in a Mini-batch in Mini-batch (MiM) sampling manner. Based on these observations, this article further discovers that generating multiplicative noise can easily degenerate the optimization due to its high dependence on the intermediate feature. Based on these studies, we propose a novel additional regularization (Addi-Reg) method, which can adaptively produce additional noise with low dependence on intermediate feature in CNNs by employing a series of mechanisms. Particularly, these well-designed mechanisms can stabilize the learning process in training, and our Addi-Reg method can pertinently learn the noise distributions for every layer in CNNs. Extensive experiments demonstrate that the proposed Addi-Reg method is more flexible and universal, and meanwhile achieves better generalization performance with faster convergence against the state-of-the-art Multi-Reg methods.

13.
J Neurotrauma ; 2021 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-33787364

RESUMO

Exosomes play an important role in intercellular communication by delivering microRNAs to recipient cells. Previous studies have demonstrated that multipotent mesenchymal stromal cell (MSC)-derived exosomes improve functional recovery after experimental traumatic brain injury (TBI). This study was performed to determine efficacy of miR-17-92 cluster-enriched exosomes (Exo-17-92) harvested from human bone marrow MSCs transfected with a miR-17-92 cluster plasmid in enhancing tissue and neurological recovery compared to exosomes derived from MSCs transfected with an empty plasmid vector (Exo-empty) for treatment of traumatic brain injury (TBI). Adult male rats were subjected to a unilateral moderate cortical contusion. Animals received a single intravenous injection of miR-17-92 cluster-enriched exosomes (100 µg/rat, approximately 3.75x1011 particles, Exo-17-92) or control exosomes (100 µg/rat, Exo-empty) or Vehicle (phosphate-buffered solution) 1 day after injury. A battery of neurological functional tests were performed weekly after TBI for 5 weeks. Spatial learning and memory were measured on days 31-35 after TBI using the Morris water maze test. All animals were sacrificed 5 weeks after injury. Their brains were processed for histopathological and immunohistochemical analyses of lesion volume, cell loss, angiogenesis, neurogenesis and neuroinflammation. Compared to the vehicle, both Exo-17-92 and Exo-empty treatments significantly improved sensorimotor and cognitive function, reduced neuroinflammation and hippocampal neuronal cell loss, promoted angiogenesis and neurogenesis without altering the lesion volume. Moreover, Exo-17-92 treatment exhibited a significantly more robust therapeutic effect on improvement in functional recovery by reducing neuroinflammation and cell loss, enhancing angiogenesis and neurogenesis than did Exo-empty treatment. Exosomes enriched with miR-17-92 cluster have a significantly better effect on improving functional recovery after TBI compared to Exo-empty, likely by reducing neuroinflammation and enhancing endogenous angiogenesis and neurogenesis. Engineering specific miRNA in exosomes may provide a novel therapeutic strategy for treatment of unilateral moderate cortical contusion TBI.

14.
Cell Res ; 2021 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-33731853

RESUMO

Neutralizing monoclonal antibodies (nAbs) to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represent promising candidates for clinical intervention against coronavirus disease 2019 (COVID-19). We isolated a large number of nAbs from SARS-CoV-2-infected individuals capable of disrupting proper interaction between the receptor binding domain (RBD) of the viral spike (S) protein and the receptor angiotensin converting enzyme 2 (ACE2). However, the structural basis for their potent neutralizing activity remains unclear. Here, we report cryo-EM structures of the ten most potent nAbs in their native full-length IgG-form or in both IgG-form and Fab-form bound to the trimeric S protein of SARS-CoV-2. The bivalent binding of the full-length IgG is found to associate with more RBDs in the "up" conformation than the monovalent binding of Fab, perhaps contributing to the enhanced neutralizing activity of IgG and triggering more shedding of the S1 subunit from the S protein. Comparison of a large number of nAbs identified common and unique structural features associated with their potent neutralizing activities. This work provides a structural basis for further understanding the mechanism of nAbs, especially through revealing the bivalent binding and its correlation with more potent neutralization and the shedding of S1 subunit.

15.
Cyberpsychol Behav Soc Netw ; 24(3): 173-181, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33646043

RESUMO

With the introduction of deepfake technology, which enables digital face-swapping between two individuals, young women are no longer passive viewers of attractive celebrities, but are able to become part of the perfect images. This study used the ZAO app as the apparatus to investigate the impact of viewing the self-celebrity deepfaked videos (SCDV) on young female users' appearance self-evaluation (i.e., body image and state appearance self-esteem). A sample of 128 young women 18-31 years of age was randomly assigned to view either 10 SCDV or 10 purely celebrity videos (PCV). All videos were sourced from the ZAO app. Results showed that participants in the SCDV condition perceived themselves as more physically attractive, experienced greater satisfaction with their own facial features, and reported marginally higher state appearance self-esteem than those in the PCV condition, whereas body shape satisfaction did not differ between the conditions. In addition, SCDV exposure increased perceived overall physical attractiveness and facial features satisfaction, but did not impact body shape satisfaction, whereas PCV exposure decreased facial features satisfaction, but did not impact perceived overall physical attractiveness or body shape satisfaction. "Attractive possible self" (APS) perception positively mediated the effect of SCDV exposure on perceived overall physical attractiveness, facial features satisfaction, body shape satisfaction, and state appearance self-esteem, while state appearance comparison only negatively mediated the effect of SCDV exposure on facial features satisfaction. This study reveals the potential of deepfake technology as an intervention technique for body image disturbances.

16.
J Agric Food Chem ; 69(10): 3154-3164, 2021 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-33666433

RESUMO

Methyl jasmonate (MeJA), a volatile organic compound, is a principal flowery aromatic compound in tea. During the processing of black tea, MeJA is produced by jasmonic acid carboxyl methyltransferase (JMT) of the jasmonic acid (JA) substrate, forming a specific floral fragrance. CsJMT was cloned from tea leaves; the three-dimensional structure of CsJMT was predicted. Enzyme activity was identified, and protein purification was investigated. Site-directed deletions revealed that N-10, S-22, and Q-25 residues in the beginning amino acids played a key functional role in enzyme activity. The expression patterns of CsJMT in tea organs differed; the highest expression of CsJMT was observed in the fermentation process of black tea. These results aid in further understanding the synthesis of MeJA during black tea processing, which is crucial for improving black tea quality using specific fragrances and could be applied to the aromatic compound regulation and tea breeding improvement in further studies.

17.
J Pharm Pharmacol ; 2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33724397

RESUMO

OBJECTIVES: Acute lung injury (ALI) is the major complication of sepsis, and no effective treatment is available now. Recently, rosmarinic acid (RA), a water-soluble polyphenolic phytochemical, exerts a potential role on ALI with anti-inflammation, and antioxidant properties. However, there is still no evidence on its protective effect on cell apoptosis in sepsis. Here, we investigated the protective effect of RA in septic-associated mortality and lung injury based on apoptosis. METHODS: Male C57BL/6 mice were administered with lipopolysaccharide (LPS) (15 mg/kg, ip) to establish ALI mice model. Preteatment of RA (20 or 40 mg/kg, ip) was performed once daily for five consecutive days. The mortality was monitored for seven days after injection of LPS. KEY FINDINGS: RA (40 mg/kg) significantly decreased mortality and alleviated septic-associated lung injury. Meanwhile, RA significantly reversed LPS induced decrease in serum T-aoc level and superoxide dismutase (SOD) activity, and increase in malondialdehyde (MDA) activity. Furthermore, RA pretreatment significantly inhibited lung cell apoptosis, as well as decreased p53 level in sepsis mice. Finally, the LPS induced activation of GRP78/IRE1α/JNK pathway was suppressed by RA pretreatment. CONCLUSIONS: These findings indicated that RA could be beneficial to septic-associated lung injury through anti-apoptosis effect.

18.
Nanomaterials (Basel) ; 11(2)2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33670815

RESUMO

A novel nano-ß-FeOOH/Fe3O4/biochar composite with enhanced photocatalytic performance and superparamagnetism was successfully fabricated via an environmentally friendly one-step method. The structural properties of the prepared composite were characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectroscopy, X-ray photoelectron spectroscopy, and a vibrating sample magnetometer. The XPS spectrum of the as-prepared composites confirmed the presence of Fe-O-C bonds between ß-FeOOH and biochar, which could be conducive to transfer photo-generated electrons. UV-vis spectroscopy confirmed the existence of an electron-hole connection between ß-FeOOH and biochar, which promoted the rapid interface transfer of photogenerated electrons from ß-FeOOH to biochar. These novel structures could enhance the response of biochar to accelerate the photoelectrons under visible light for more free radicals. Electron spin resonance analysis and free radical quenching experiments showed that •OH was the primary active species in the photodegradation process of methyl orange by nano-ß-FeOOH/Fe3O4/biochar. In the synergistic photocatalytic system, ß-FeOOH/Fe3O4/biochar exhibited excellent catalytic activity for the degradation of azo dye (methyl orange), which is 2.03 times higher than that of the original biochar, while the surface area decreased from 1424.82 to 790.66 m2·g-1. Furthermore, ß-FeOOH/Fe3O4/biochar maintained a stable structure and at least 98% catalytic activity after reuse, and it was easy to separate due to its superparamagnetism. This work highlights the enhanced photocatalytic performance of ß-FeOOH/Fe3O4/biochar material, which can be used in azo dye wastewater treatment.

19.
Sci Rep ; 11(1): 5731, 2021 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-33707604

RESUMO

Physical non-communicable diseases (NCDs) and mental health disorders are a rapidly increasing health burden in low-and middle-income countries. This study aims to examine the relationships between mental health disorders and cascade of care in managing four common physical NCDs (hypertension, diabetes, dyslipidemia, chronic kidney disease) in China. We utilized two waves of nationally-representative China Health and Retirement Longitudinal Study (CHARLS 2011, 2015) of older adult population aged 45 and above. A series of unadjusted and adjusted mixed-effect logistic regression was applied to evaluate the association between presence of mental health disorder and physical chronic disease awareness, treatment, and control. We found that the odds of dyslipidemia (AOR 1.81, 95% CI 1.36-2.39) and kidney disease awareness (AOR 2.88, 95% CI 2.12-3.92) were higher for individuals with mental chronic conditions, compared to those without mental chronic conditions. The odds of having hypertension treatment was higher for subjects with mental health disorder, compared to those without (AOR 1.32, 95% CI 1.02-1.70). The odds of having physical chronic conditions controlled was not significantly associated with having mental chronic conditions (P > 0.05). These results indicated that adults with mental health disorder have a greater likelihood of awareness of having dyslipidemia and kidney disease, and receiving treatment for hypertension. Strategies to address the growing burden of physical-mental NCDs in China should include efforts to improve management of patients with comorbid health condition and improve access to continual high-quality treatment after the first diagnosis.

20.
Nucleic Acids Res ; 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33693821

RESUMO

RNA-protein interactions play key roles in epigenetic, transcriptional and posttranscriptional regulation. To reveal the regulatory mechanisms of these interactions, global investigation of RNA-binding proteins (RBPs) and monitor their changes under various physiological conditions are needed. Herein, we developed a psoralen probe (PP)-based method for RNA tagging and ribonucleic-protein complex (RNP) enrichment. Isolation of both coding and noncoding RNAs and mapping of 2986 RBPs including 782 unknown candidate RBPs from HeLa cells was achieved by PP enrichment, RNA-sequencing and mass spectrometry analysis. The dynamics study of RNPs by PP enrichment after the inhibition of RNA synthesis provides the first large-scale distribution profile of RBPs bound to RNAs with different decay rates. Furthermore, the remarkably greater decreases in the abundance of the RBPs obtained by PP-enrichment than by global proteome profiling suggest that PP enrichment after transcription inhibition offers a valuable way for large-scale evaluation of the candidate RBPs.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...