Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 68
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Hazard Mater ; 382: 121091, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31472465

RESUMO

In this study, we proposed to apply an integrated process which is comprised of in situ ozonation, ceramic membrane filtration (CMF) and biologically active carbon (BAC) filtration to wastewater reclamation for indirect potable reuse purpose. A pilot-scale (20 m3/d) experiment had been run for ten months to validate the prospect of the process in terms of treatment performance and operational stability. Results showed that the in situ O3 + CMF + BAC process performed well in pollutant removal, with chemical oxygen demand, ammonia, nitrate nitrogen, total phosphorus and turbidity levels in the treated water being 5.1 ±â€¯0.9, 0.05 ±â€¯0.01, 10.5 ±â€¯0.8, <0.06 mg/L, and <0.10 NTU, respectively. Most detected trace organic compounds were degraded by>96%. This study demonstrated that synergistic effects existed in the in situ O3 + CMF + BAC process. Compared to pre-ozonation, in situ ozonation in the membrane tank was more effective in controlling membrane fouling (maintaining operational stability) and in degrading organic pollutants, which could be attributed to the higher residual ozone concentration in the tank. Because of the removal of particulate matter by CMF, water head loss of the BAC filter increased slowly and prolonged the backwashing interval to 30 days. BAC filtration was also effective in removing ammonia and N-nitrosodimethylamine from the ozonated water.

2.
Oncol Rep ; 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31524278

RESUMO

Colorectal cancer (CRC), a commonly occurring malignant tumor in the gastrointestinal tract, is the third leading cause of cancer­related deaths worldwide. FK506­binding proteins (FKBPs) comprise an immunophilin family that are involved in the carcinogenesis, progression and chemoresistance of cancers, including CRC. FKBP3 (also known as FKBP25) is a nuclear protein that is a member of the FKBP family and is correlated with the activity of histone deacetylase 2 (HDAC2). However, the role of FKBP3 and HDAC2 in oxaliplatin resistance in CRC and the potential molecular mechanisms are still poorly understood. In the present study, the expression of FKBP3, HDAC2 and related­genes was detected by real­time PCR and western blot analysis. Furthermore, cell apoptosis was detected by flow cytometry (FCM). We found high expression of FKBP3 and HDAC2 in CRC tissues. In vitro, primary CRC cells with high expression of FKBP3 and HDAC2 were insensitive to oxaliplatin. Downregulation of FKBP3 significantly increased the sensitivity of primary CRC cells to oxaliplatin, reduced expression of HDAC2, permeability glycoprotein (P­gp) and phosphorylated AKT (p­AKT), and increased expression of phosphatase and tensin homolog (PTEN) and cleaved caspase­3. Accordingly, upregulation of FKBP3 had the opposite effect. Furthermore, downregulation of HDAC2 significantly counteracted FKBP3­induced oxaliplatin resistance in CRC cells. Our data revealed that oxaliplatin resistance in CRC cells is positively associated with FKBP3 and HDAC2 expression, and FKBP3 downregulation could attenuate oxaliplatin resistance in CRC cells by reducing HDAC2 expression and possibly through regulation of the PTEN/AKT pathway.

3.
J Environ Sci (China) ; 85: 189-199, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31471026

RESUMO

Membrane fouling is an obstacle impeding the wide applications of ceramic membranes and organics are responsible for most of the membrane fouling issues in wastewater treatment. In this study, Fenton cleaning strategy was firstly proposed to clean ceramic membrane fouling in wastewater treatment. Fe2+ efficiently catalyzed fouling cleaning with H2O2 (1.5%) to recover the filterability of ceramic membrane. The maximum ∆TMP recovery (over 99%) was achieved at an optimal Fe2+ dosage of 124 mg/L after 6 hr of immersion cleaning. The total residual membrane fouling resistance decreased gradually from this optimum value as the Fe2+ dosage increased above 124 mg/L. The residual hydraulically reversible fouling resistance accounted for most of the membrane fouling and was basically removed (≤3.0 × 109 m-1) when Fe2+ dosages higher than 124 mg/L were used. The foulants responsible for the formation of a residual hydraulically reversible fouling layer (DOC (dissolved organic carbon), proteins, polysaccharides, EEM (fluorescence excitation-emission matrix spectra), SS (suspended solids), and VSS (volatile suspended solids)) were gradually removed as the Fe2+ dosage increased. These residual organic foulants were degraded from biopolymers (10-200 kDa) to low molecular weight substances (0.1-1 kDa), and the particle size of these residual foulants decreased significantly as a result. The strong oxidation power of hydrogen peroxide/hydroxy radicals towards organic foulants was enhanced by Fe2+. Fe2+ played a significant role in the removal of hydraulically reversible fouling and irreversible fouling from the ceramic membrane. However, Fe2+ (≥124 mg/L) increased the likelihood of forming secondary iron-organics aggregates.


Assuntos
Membranas Artificiais , Eliminação de Resíduos Líquidos/métodos , Biopolímeros , Cerâmica , Peróxido de Hidrogênio , Águas Residuárias
4.
BMC Immunol ; 20(1): 28, 2019 08 07.
Artigo em Inglês | MEDLINE | ID: mdl-31390976

RESUMO

BACKGROUND: The aim of the current study was to investigate the long non-coding RNA (lncRNA) expression profiles in psoriatic arthritis (PSA) patients by RNA sequencing, and to further explore potential biomarkers that were able to predict PSA risk and activity. METHODS: LncRNA and mRNA expression profiles in peripheral blood mononuclear cells (PBMC) of 4 PSA patients and 4 normal controls (NCs) were detected by RNA sequencing, followed by comprehensive bioinformatic analyses. Subsequently, 3 top upregulated and 2 top downregulated lncRNAs were chosen for further validation in 93 PSA patients and 93 NCs by quantitative polymerase chain reaction (qPCR) assay. RESULTS: Totally 76 upregulated and 54 downregulated lncRNAs, as well as 231 upregulated and 102 downregulated mRNAs were discovered in PSA patients compared with NCs. Enrichment analyses revealed that they were mostly associated with nucleosome, extracellular exosome and extracellular matrix, and the top enriched pathways were systemic lupus erythematosus (SLE), alcoholism and viral carcinogenesis. qPCR assay showed that lnc-RP11-701H24.7 and lnc-RNU12 were upregulated in PSA patients compared with NCs, and they could predict PSA risk with high area under curves. Besides, lnc-RP11-701H24.7 was positively associated with ESR, SJC, TJC and pain VAS score while lnc-RNU12 was positively correlated with PASI score, CRP and PGA score, implying that both of them were positively correlated with disease activity. CONCLUSION: Our study facilitates comprehensive understanding of lncRNA expression profiles in PSA pathogenesis, and discovers that lnc-RP11-701H24.7 and lnc-RNU12 might be served as novel biomarkers for PSA risk and activity.

5.
Acta Biochim Biophys Sin (Shanghai) ; 51(8): 826-833, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31287493

RESUMO

Esophageal squamous cell carcinoma (ESCC) is a common malignancy with poor prognosis. The drug resistance compromises the efficacy of chemotherapy for ESCC. Long non-coding RNA taurine upregulated gene 1 (TUG1) has been identified as a promoter of cancer progression and chemotherapy resistance in many malignancies. However, the exact role of TUG1 in ESCC chemotherapy resistance remains unclear. In this study, we showed that TUG1 expression in TE-1-derived cisplatin (DDP)-resistant (TE-1/DDP) cells was higher than that in TE-1 cells. Furthermore, TUG1 promoted DDP resistance in TE-1 and TE-1/DDP cells by promoting cell proliferation, suppressing cell apoptosis, and elevating protein expression of the classical multi-drug resistance-related P-gp. In contrast, TUG1 knockdown exerted an opposite effect. Mechanistically, RNA pull-down and RNA immunoprecipitation assays confirmed that TUG1 directly bound to nuclear factor (erythroid-derived 2)-like 2 (Nrf2) protein and elevated Nrf2 protein expression. Moreover, Nrf2-neutralizing antibody effectively reversed the TUG1 overexpression-mediated promotion of ESCC cell resistance to DDP. In conclusion, our findings demonstrated that TUG1 promoted ESCC cell resistance to DDP, at least in part, through upregulating Nrf2.

6.
Water Res ; 160: 445-453, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31174072

RESUMO

Technologies capable of selective removal of target contaminants from water are highly desirable to achieve "fit-for-purpose" treatment. In this study, we developed a simple yet highly effective method to achieve calcium-selective removal in an electrosorption process by coating the cathode with a calcium-selective nanocomposite (CSN) layer using an aqueous phase process. The CSN coating consisted of nano-sized calcium chelating resins with aminophosphonic groups in a sulfonated polyvinyl alcohol hydrogel matrix, which accomplished a Ca2+-over-Na+ selectivity of 3.5-5.4 at Na+:Ca2+ equivalent concentration ratio from 10:1 to 1:1, 94 - 184% greater than the uncoated electrode. The CSN coated electrode exhibited complete reversibility in repeated operation. Mechanistic studies suggested that the CSN coating did not contribute to the adsorption capacity, but rather allowed preferential permeation of Ca2+ and hence increased Ca2+ adsorption on the carbon cathode. The CSN-coated electrode was very stable, showing reproducible performance in 60 repeated cycles.


Assuntos
Nanocompostos , Purificação da Água , Adsorção , Cálcio , Eletrodos , Íons , Água
7.
Biosci Rep ; 39(5)2019 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-30967496

RESUMO

Colon cancer is a leading cause of cancer-related deaths worldwide. The epithelial-mesenchymal transition (EMT) plays an important role in tumor metastasis of colon cancer. We first evaluated the effects of EMT-related transcription factors on the prognosis of colon cancer through analysis the data obtained from The Cancer Genome Atlas (TCGA). And then we screened a series of Chinese medicine monomers to find effect EMT inhibitors. First, Snail is a more important EMT transcription factors for colon cancer prognosis, compared with Twist and Slug. Then, we found that apigenin effectively inhibits the activity of Snail. Apigenin could inhibit the EMT, migration, and invasion of human colon cancer cells in vitro and in vivo through the NF-κB/Snail pathway. Snail is a key regulator of EMT in colon cancer and Snail inhibitor apigenin may be a therapeutic application for patients with colon cancer.

8.
Sci Total Environ ; 664: 116-126, 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-30739846

RESUMO

Ferrous dosing is used to reduce phosphorus concentration and alleviate polymeric membrane fouling in membrane bioreactor (MBR). However, limited studies have been conducted to investigate the impacts of ferrous dosing on ceramic membrane fouling, nutrient removal efficiency and microbial community. Accordingly, the aim of this study was to investigate the effect of intermittent ferrous dosing with Fe/P molar ratios of 2 and 1 (with a dosing frequency of every two days) on the overall nutrient removal, functional microbial changes and membrane fouling in ceramic membrane bioreactors (CMBR) in treatment of wastewater. TP concentration of 10 mg/L in influent decreased to 1.94 ±â€¯0.62 mg/L (control), 0.38 ±â€¯0.22 mg/L (Fe/P = 1) and 0.31 ±â€¯0.18 mg/L (Fe/P = 2) in the effluent, respectively. Meanwhile, the effluent total nitrogen (TN) concentrations with Fe/P = 1 treatment (6.80 ±â€¯2.02 mg/L) and Fe/P = 2 treatment (5.12 ±â€¯2.28 mg/L) were lower than that of the control (7.72 ±â€¯2.36 mg/L). Compared to Fe/P = 1, the TN removal performance was better for Fe/P = 2 mainly due to the increased abundance of denitrifying bacteria (Zoogloea and Acinetobacter). In addition, excess iron dose might have toxic effects on bacterial physiology, however the Fe concentrations that cause cell damage vary for different bacteria. The relative abundance of Zoogloea (aerobic denitrifying bacteria) continuously increased with ferrous addition (Fe/P = 2), while other bacteria including Dechloromonas, Hyphomicrobium and Thauera (anoxic denitrifying bacteria), Nitrospira (nitrifying bacteria) and Candidatus Accumulibacter (phosphorus accumulating organism) decreased sharply. Furthermore, membrane fouling was effectively moderated by ferrous dosing and Fe/P = 1 treatment showed improved membrane fouling mitigation than Fe/P = 2. Overall, intermittent ferrous addition in CMBR with Fe/P molar ratio of 1 was beneficial to the removal of nutrients (TP, TN and organics), enhanced succession of microbial community and membrane fouling mitigation.


Assuntos
Reatores Biológicos , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/química , Cerâmica , Desnitrificação , Compostos Ferrosos , Membranas Artificiais , Microbiota , Fósforo
9.
JAMA Dermatol ; 155(3): 327-334, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30698628

RESUMO

Importance: It is necessary to determine whether psoriasis responds to methotrexate in the same manner in patients with and without psoriatic arthritis. Objective: To evaluate the effectiveness and safety of methotrexate in treating patients with psoriasis with and without psoriatic arthritis. Design, Setting, and Participants: In this prospective, single-arm, interventional study, a total of 235 patients with psoriasis, 107 without psoriatic arthritis and 128 with psoriatic arthritis who were receiving methotrexate therapy from April 1, 2015, to December 31, 2017, were recruited from the outpatient department of a hospital at a large Chinese university. There were no significant demographic or clinical differences between the subgroups with the exception of diabetes. Interventions: A 12-week course of low-dosage oral methotrexate (7.5-15 mg weekly). Main Outcomes and Measures: Changes in disease severity, adverse events, blood cell counts, and liver and renal function. Results: A total of 235 patients with psoriasis (166 male [66.0%]; mean [SD] age, 49.6 [15.1] years) received methotrexate treatment for 12 weeks. The 90% reduction from baseline Psoriasis Area Severity Index response was significantly lower in patients with psoriatic arthritis than in patients without psoriatic arthritis at week 8 (4 0f 128 [3.1%] vs 12 of 107 [11.2%]; P = .02) and week 12 (19 of 128 [14.8%] vs 27 of 107 [25.2%]; P = .049). Furthermore, the incidence of adverse events, including dizziness (12 of 128 [9.4%] vs 1 of 107 [0.9%]; P = .007), gastrointestinal symptoms (32 of 128 [25.0%] vs 13 of 107 [12.1%]; P = .01), and hepatoxicity (34 of 128 [26.6%] vs 16 of 107 [15.0%]; P = .04), was significantly higher in patients with psoriatic arthritis than in patients without psoriatic arthritis. Methotrexate-induced elevation of alanine aminotransferase levels was associated with body mass index (mean [SD] body mass index, 26 [4] in patients with [P = .04] vs 26 [4] in those without [P = .005] psoriatic arthritis) and smoking (17 of 34 [50.0%] in patients with [P = .02] vs 9 of 16 [56.3%] in those without [P = .04] psoriatic arthritis). Conclusions and Relevance: In this study, methotrexate was well tolerated and effective in treating psoriasis. It was more effective, with fewer adverse effects, in patients with psoriasis who did not have psoriatic arthritis than in patients who presented with both psoriasis and psoriatic arthritis. Therefore, methotrexate can be recommended as first-line treatment for psoriasis without arthritis.

10.
Psychol Health Med ; 24(3): 269-280, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30293440

RESUMO

Previous studies have reported a higher incidence of depression and anxiety in psoriasis patients compared to the general population, which has important implications for assessment and treatment. In this study, we determined the frequency of depression and anxiety in Chinese patients with psoriasis and its relationship with disease severity and other demographic variables. The study included 208 Chinese patients with psoriasis vulgaris. The Patient Health Questionnaire (PHQ-9) and Generalized Anxiety Disorder scale (GAD-7) were used to screen for depression and anxiety. The Psoriasis Area and Severity Index (PASI) was used to assess the severity of psoriasis. Of the 208 patients included in the study, 29 patients (13.9%) were positive for moderate-to-severe depression (PHQ-9 ≥ 10) and 22 patients (10.6%) were positive for anxiety (GAD-7 ≥ 10) symptoms. Both positive stress reactors who perceived stress as an exacerbating factor of psoriasis and moderate-to-severe psoriasis were found to be positive predictors for the presence of moderate-to-severe depression or anxiety symptoms while longer duration and late onset age played a protective role. In the sample of Chinese patients with psoriasis there was a clinically significant prevalence of depression and anxiety. Our study suggests that Chinese psoriasis patients should be screened for psychiatric comorbidities.


Assuntos
Transtornos de Ansiedade/epidemiologia , Ansiedade/epidemiologia , Depressão/epidemiologia , Transtorno Depressivo/epidemiologia , Psoríase/epidemiologia , Estresse Psicológico/epidemiologia , Adulto , China/epidemiologia , Comorbidade , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Tempo , Adulto Jovem
11.
Front Biosci (Landmark Ed) ; 24: 303-312, 2019 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-30468657

RESUMO

Oxaliplatin is one of the most common chemotherapy drugs for colorectal cancer (CRC), but its application is greatly limited owing to the drug resistance. Nobiletin is a natural flavonoid isolated from citrus peel and has many biological functions, including anti-inflammatory, antitumor and neuroprotective activities. However, little is known about the effect of nobiletin on the anti-tumor activities of other chemotherapy drugs. In this study, we examined the effect of nobiletin on the efficacy of oxaliplatin in treatment of CRC by using two CRC cell lines. In vitro experiments indicated that nobiletin enhanced the inhibitory effect of oxaliplatin on the proliferation of CRC cells. Meanwhile, nobiletin promoted oxaliplatin-induced apoptosis of CRC cells, as demonstrated by the increased expression of pro-apoptotic proteins (Bax and cleaved-caspse3) and the down-regulation of anti-apoptotic protein Bcl-2. Mechanically, nobiletin sensitized CRC to oxaliplatin chemotherapy by down-regulating the PI3K/Akt/mTOR pathway. Taken together, our study has demonstrated that nobiletin could enhance the sensitivity of CRC to oxaliplatin chemotherapy, and provided a molecular basis for nobiletin's potential applications in the chemosensitization of CRC.


Assuntos
Flavonas/farmacologia , Oxaliplatina/farmacologia , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Serina-Treonina Quinases TOR/metabolismo , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Sinergismo Farmacológico , Células HT29 , Humanos
12.
Sci Total Environ ; 652: 788-799, 2018 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-30380486

RESUMO

The comparison of long-term ceramic membrane bioreactors (MBRs) without and with in-situ ozonation was investigated in this study in terms of membrane fouling, activated sludge, effluent quality and microbial community in wastewater treatment. The optimal dosage of in-situ ozonation for long-term MBR operation was firstly determined as 5 mg/L (0.66 mg-ozone/g-mixed liquor suspended solid (MLSS)) with the optimal filterability of mixed liquor. During the long-term filtration experiment, MBR-ozone with in-situ ozonation demonstrated its significantly alleviated ceramic membrane fouling performance compared with MBR-control without in-situ ozonation as a result of the enhanced filterability of mixed liquor and organic foulants removal from membrane surface by in-situ ozonation oxidation. Furthermore, ozonation was beneficial to phosphorus removal and the total phosphorus (TP) concentration in effluent of MBR-control (0.82 ±â€¯0.63 mg/L) was >2-fold higher than that of MBR-ozone (0.29 ±â€¯0.41 mg/L). The improved phosphorus removal performance by ozonation was due to the increased abundance of phosphate accumulating bacteria of Candidatus Accumulibacter in activated sludge. However, ozonation was detrimental to nitrogen removal mainly as a result of the inhibition of denitrification with the decreased relative abundance of denitrification genus of Dechloromonas in activated sludge. Overall, ceramic MBR with in-situ ozonation had not only significantly alleviated membrane fouling but also remarkably improved phosphorus removal performance.

13.
J Colloid Interface Sci ; 537: 247-255, 2018 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-30448645

RESUMO

Capacitive deionization (CDI) has become a promising technology for water desalination due to its remarkable advantages including low operation cost, no secondary pollution and high rate of ion recovery. However, the majority of commercial CDI electrode materials are carbonaceous materials such as activated carbon with limited capacitance and high charge transfer resistance, which significantly hinders the wide application of CDI. Herein, we demonstrate a N-doped carbonaceous CDI electrode with a maximum ion electrosorption capacity of 19.9 mg/g, a low charge transfer resistance (1.17 Ω) and a robust regeneration performance (2800 min for 28 circles). The N-doped carbonaceous CDI electrode is the commercial activated carbon fiber (ACF) decorated with polyaniline (PANI) (ACF/PANI) electrode fabricated by in-situ electrochemical polymerization. The ACF/PANI electrode was characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), and fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The pseudocapacitance of ACF/PANI electrode significantly contributed to the effectively improved CDI performance that 90.0% of sodium storage was attributed to the capacitive process and the unique porous structure of ACF/PANI electrode contributed to the other 10.0% diffusion-controlled capacity.

14.
Medicine (Baltimore) ; 97(39): e11550, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30278479

RESUMO

The clinical significance of palliative interventional therapy in the management of patients with advanced hilar cholangiocarcinoma (HCCA; stages III-IV) has yet to be studied. The present work was aimed to compare the clinical outcomes of the patients treated with surgery or interventional therapy.A total of 90 patients with advanced HCCA, who admitted Fuyang First People's Hospital from May 2015 to February 2016, were enrolled. Forty-five of them were assigned to the experimental group receiving biliary drainage as the interventional therapy, and the remaining 45 patients were designated as the conventional group receiving radical/palliative surgery. Before and after the treatment total bilirubin from blood was measured. The length of treatment and medical cost were also examined. All patients were followed up for at least 1 year after the treatment.For both the experimental and conventional groups, the serum bilirubin levels after treatment were significantly lower than those before treatment (P < .05); however, no significant differences between groups were seen. There were no significant differences between experimental and conventional groups in the incidence of postoperative complications and survival outcomes. Of note, the length of treatment of the experimental group was substantially shorter than that of the conventional group (P < .05). The medical expense of the experimental group was only about one-third of that of the conventional group (P < .05).Although the interventional therapy does not improve patients' survivals and reduce the incidence of complications, it significantly shortens the treatment length, reducing substantially the medical expense. This finding provides new insights into the treatment strategy for patients with advanced HCCA.


Assuntos
Neoplasias dos Ductos Biliares/cirurgia , Colangiocarcinoma/cirurgia , Drenagem/métodos , Cuidados Paliativos/métodos , Adulto , Idoso , Bilirrubina/sangue , Drenagem/efeitos adversos , Feminino , Seguimentos , Custos de Cuidados de Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/epidemiologia , Taxa de Sobrevida , Resultado do Tratamento , Adulto Jovem
15.
J Dermatol Sci ; 92(1): 106-113, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30072243

RESUMO

BACKGROUND: Few studies have explored the differences of immunopathogenesis in plaque vs guttate psoriasis, especially on the inhibitory role of regulatory T cells (Tregs) on IL-17/ IFN-γ production and the impact of CD4+T cells on keratinocytes. OBJECTIVE: To investigate the percentage and inhibitory function of CD4+CD25highTreg and differential expressions of IL-17/ IFN-γ in plaque vs guttate psoriasis, and the effects of CD4+T cells on the proliferation of keratinocytes. METHODS: Peripheral blood mononuclear cells (PBMCs) were prepared from patients with the plaque and guttate psoriasis. The percentage of CD4+CD25high Tregs, IL-17/IFN-γ- producing CD4+ or CD8+T cells, and apoptosis and cell cycle of Hacat cells were determined by flow cytometry. The level of IFN-γ in supernatants was analyzed by ELISA. RESULTS: The percentage of CD4+CD25highTregs in plaque psoriasis was significantly increased, and they can inhibit IFN-γ production from CD4+CD25- effector T cells. The percentage of CD8+IFN-γ+cells was also significantly increased in plaque psoriasis, and these cells positively correlated with disease severity. The percentage of CD4+CD25highTregs was decreased and CD4+IFN-γ+/IFN-γ+IL-17+ cells were predominantly increased in guttate psoriasis. CD4+T cells from guttate psoriasis induce apoptosis of keratinocytes while they promote the proliferation of keratinocytes in plaque psoriasis by decreasing late apoptosis and increasing the percentage of G1 phase. CONCLUSION: There was considerable discrepancy of the phenotype and function of T cells between plaque vs guttate psoriasis. IFN-γ and IL-17 from CD4+T cells play a crucial role in guttate psoriasis, however, IFN-γ and IL-17 from CD8+T cells are more important in the immunopathogenesis of plaque psoriasis.

16.
Front Chem ; 6: 313, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30137746

RESUMO

Hypophosphite wastewater treatment is still a critical issue in metallurgical processes and the oxidation of hypophosphite to phosphate followed by the precipitation of phosphate is an important strategy for hypophosphite wastewater treatment. Herein, Ti4O7/g-C3N4 photocatalysts with various mass ratios (Ti4O7 (m): g-C3N4 (m) = 0.5, 0.2, 0.1, and 0.05) were synthesized by a hydrolysis method and the effect of the mass ratio of Ti4O7 (m): g-C3N4 (m) on Ti4O7/g-C3N4 visible light photocatalytic oxidation of hypophosphite was evaluated. The as-prepared Ti4O7/g-C3N4 were characterized and confirmed by SEM, XPS, XRD and FTIR. Moreover, the specific surface area and the distribution of pore size of Ti4O7/g-C3N4 was also analyzed. Our results showed that Ti4O7/g-C3N4 exhibited remarkably improved photocatalytic performance on hypophosphite oxidation compared with g-C3N4 and meanwhile 1:2-Ti4O7/g-C3N4 with a mass ratio of 0.5 showed the best photocatalytic performance with the highest oxidation rate constant (17.7-fold and 91.0-fold higher than that of pure g-C3N4 and Ti4O7, respectively). The enhanced performance of photocatalytic oxidation of hypophosphite was ascribed to the heterojunction structure of Ti4O7/g-C3N4 with broader light absorption and significantly enhanced efficiency of the charge carrier (e--h+) generation and separation. Additionally, the generated ·OH and · O2- radicals contributed to the hypophosphite oxidation during the photocatalytic system.

17.
Mol Ther Methods Clin Dev ; 10: 57-67, 2018 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-30069494

RESUMO

Extensive studies on tumor suppressor genes (TSGs) are helpful to understand the pathogenesis of cancer and design effective treatments. However, identifying TSGs using traditional experiments is quite difficult and time consuming. Developing computational methods to identify possible TSGs is an alternative way. In this study, we proposed two computational methods that integrated two network diffusion algorithms, including Laplacian heat diffusion (LHD) and random walk with restart (RWR), to search possible genes in the whole network. These two computational methods were LHD-based and RWR-based methods. To increase the reliability of the putative genes, three strict screening tests followed to filter genes obtained by these two algorithms. After comparing the putative genes obtained by the two methods, we designated twelve genes (e.g., MAP3K10, RND1, and OTX2) as common genes, 29 genes (e.g., RFC2 and GUCY2F) as genes that were identified only by the LHD-based method, and 128 genes (e.g., SNAI2 and FGF4) as genes that were inferred only by the RWR-based method. Some obtained genes can be confirmed as novel TSGs according to recent publications, suggesting the utility of our two proposed methods. In addition, the reported genes in this study were quite different from those reported in a previous one.

18.
Nanoscale Res Lett ; 13(1): 66, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29605867

RESUMO

A convenient, low-cost, and highly sensitive fluorescent aptasensor for detection of leukemia has been developed based on graphene oxide-aptamer complex (GO-apt). Graphene oxide (GO) can absorb carboxyfluorescein-labeled Sgc8 aptamer (FAM-apt) by π-π stacking and quench the fluorescence through fluorescence resonance energy transfer (FRET). In the absence of Sgc8 target cell CCRF-CEM, the fluorescence is almost all quenched. Conversely, when the CCRF-CEM cells are added, the quenched fluorescence can be recovered rapidly and significantly. Therefore, based on the change of fluorescence signals, we can detect the number of CCRF-CEM cells in a wide range from 1 × 102 to 1 × 107 cells/mL with a limit of detection (LOD) of 10 cells/mL. Therefore, this strategy of graphene oxide-based fluorescent aptasensor may be promising for the detection of cancer.

19.
Nanoscale Res Lett ; 13(1): 117, 2018 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-29691667

RESUMO

In the original publication of this article [1] the author Liping Zhong was omitted. In this correction article the author and the corresponding details are provided. The publisher apologizes to the readers and authors for the inconvenience.

20.
J Biomed Nanotechnol ; 14(3): 575-584, 2018 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-29663929

RESUMO

Effector memory T cells (TEM) are a subset of memory T cells which play an important role in stimulation of adaptive immunity. Although they are associated with multiple immune responses, the antitumor effect of TEM is not clearly understood. In this research, generation of anti-tumor TEM was induced through skin allografts in C57BL/6 mice with B16 melanoma. We observed that the growth of tumor tissues in C57BL/6 mice treated with allografts was interrupted. Furthermore, the survival time for the treated mice was prolonged along with increased serum levels for CXCL9, CXCL10 and INF-γ. Additionally, the concentrations of TEM in the spleens, lymph nodes and tumor tissues were markedly elevated in allografts treated mice. The tumor cell proliferation and tissue growth were suppressed in C57BL/6 mice with B16 melanoma induced by allografts, upon tail vein injection of purified TEM. These results demonstrate that skin allografts promote the generation of anti-tumor TEM in C57BL/6 mice with B16 melanoma, which emphasize the strong promise of TEM stimulation using allografts transplants in effective tumor immunotherapy.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA