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1.
Artigo em Inglês | MEDLINE | ID: mdl-32876434

RESUMO

The on-chip system-compatible power supply shows a high demand for the rapid development of miniaturization devices, such as wireless sensors, remote detecting devices, etc. Moreover, the ever-increasing trends of multifunctionalities and long-term working conditions of such devices raise a high-performance standard for the power supply. Herein, the high-performance electrochemical energy storage micro-supercapacitors (MSCs) are obtained with a metal current collector-free symmetric graphene-based planar structure, in which the functional group of graphene was regulated extensively via fully compatible microfabrication techniques of blue-violet (BV) laser exposure and air plasma treatment. BV laser exposure enhanced the electrical conductivity by reducing the substantial functional groups. Furthermore, the wettability and active sites are tuned by air plasma treatment, thus creating a slightly functional group onto the graphene surface. The resulting reduced graphene oxide (RGO) shows a very low resistance down to 27.2 Ω sq-1, ensuring its superb electron conductivity for fast electron transfer during the electrochemical reactions. The electrochemical performance measurements reveal an areal capacitance as high as 21.86 mF cm-2, which delivers a power density of 5 mW cm-2 with an energy density of 2.49 µWh cm-2. Moreover, it shows superior long-term stability with 99% retention after 10 000 cycles, which is beyond that of most of the reported graphene-based all-solid-state MSCs.

3.
Sci Total Environ ; 742: 140609, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-32721739

RESUMO

Fiddler crabs (Uca) are ecosystem engineers in coastal ecosystems. Many anthropogenic and natural factors can affect the time allocated to various behaviours in Uca. However, the behaviour of U. arcuata, a widely distributed fiddler crab in Asia, has not been studied in mainland China. Here, we used binoculars to record the time budget of ten behaviours of U. arcuata to investigate the potential effects of sex, tides, and pollution on these behaviours. We found that the crabs spent 42.3%, 27.0%, and 10.6% of their time on feeding, feeding while walking, and stationary respectively. The crabs spent <1.5% of their time on copulation and grooming. The total foraging time (feeding + feeding while walking) did not differ among the three polluted sites. However, crabs spent more time on feeding but less time on feeding while walking. The feeding rate and probability of burrowing and grooming decreased while the possibility of locomotion and stay in burrow increased with increasing nutrient concentration. Females spent 13.9% more time on feeding and fed 54.9% faster than males. Males had a higher tendency to grooming and combat while they were less likely to walk than females. Regarding to the influence of tide, fiddler crabs fed 11.2% faster at ebb tides than at flood tides, and they were more likely to walk and stay in burrows at flood tides than at ebb tides. Our results indicated that nutrient pollution had stronger impacts on the behaviours of crabs than sex and tide. In polluted mangroves, increasing nutrient concentration reduced the quantity of sediment processed by fiddler crabs due to their smaller feeding area, slower feeding rate, and reduced frequency of burrowing activities. These results imply that mitigating nutrient pollution in mangroves may benefit the restoration and management of coastal ecosystems through the enhanced engineering functions of fiddler crabs.


Assuntos
Braquiúros , Animais , Ásia , China , Ecossistema , Feminino , Masculino , Comportamento Espacial
4.
BMC Urol ; 20(1): 94, 2020 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-32650766

RESUMO

BACKGROUND: The roles of lncRNA PLAC2 in bladder cancer (BC) were explored. METHODS: The expression of PLAC2 in two types of tissue of BC patients was detected by RT-qPCR and the expression data were compared by paired t test. The 56 patients were staged according to the AJCC criteria, and 12, 15, 15 and 14 cases were classified into stage I-IV, respectively. The expression of TGF-ß1 and miR-663 in BC tissues were also detected by RT-qPCR experiments. RESULTS: Our data showed that the expression levels of PLAC2 were significantly lower in BC tissues than that in non-cancer tissues. The expression of PLAC2 was not affect by clinical stages and low expression levels of PLAC2 predicted lower survival rate. The expression of PLAC2 was positively correlated with miR-663 and inversely correlated with TGF-ß1 in BC tissues. In BC cells, downregulated TGF-ß1 and upregulated miR-663 were observed after the overexpression of PLAC2. Overexpression of PLAC2 also resulted in suppressed invasion and migration of BC cells. Overexpression of miR-663 resulted in downregulated TGF-ß1 but did not affect the expression of PLAC2. Overexpression of TGF-ß1 reduced the inhibitory effects of overexpression of PLAC2 and miR-663 on cell migration and invasion. CONCLUSION: PLAC2 can upregulate miR-663 to downregulate TGF-ß1 and suppress BC cell migration and invasion.

5.
Arch Osteoporos ; 15(1): 90, 2020 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-32556596

RESUMO

Renal tubule cells play a pivotal role in maintaining bone homeostasis. Hence, renal tubular function may be associated with bone mineral density. Our study found that urinary ß2 microglobulin-creatinine ratio (UBCR) levels correlated negatively with lumbar spine bone mineral density (BMD) and T and Z values, and may be a marker for osteoporosis in Chinese elderly male adults. PURPOSE: To study the association of UBCR levels with BMD and the predictive value of UBCR for osteoporosis in elderly Chinese male adults. METHODS: A cross-sectional study of 149 (65 to 85 years, 69.7 ± 4.6) Chinese male adults who underwent health checkups in Huadong Hospital in Shanghai China was conducted. BMD was measured by dual-energy X-ray absorptiometry. The clinical variables and BMD of the participants in the low UBCR group (B1, UBCR < 300 µg/g) and the high UBCR group (B2, UBCR ≥ 300 µg/g) were compared. Associations between UBCR with clinical variables and BMD were analyzed by Pearson's correlation coefficient and multiple regression analysis. BMD and T and Z values were compared between the B1 and B2 groups. The odds ratios (ORs) for dose-dependent increases in osteoporosis between B1 and B2 were analyzed by binary logistic regression analysis. The area under the receiver operating characteristic (ROC) curve was used to analyze the capacity of UBCR to predict osteoporosis. RESULTS: UBCR was significantly higher in the osteoporosis group. After adjusting for multiple confounders, UBCR levels correlated negatively with BMD and T and Z values of the lumbar spine. Lumbar spine BMD and T and Z values were significantly lower in the B2 UBCR group than in the B1 UBCR group. Compared with the B1 participants, the ORs for "osteoporosis" were 12.401 times higher in B2 participants (P = 0.005) by binary logistic regression analysis after adjusting for potential confounders. The UBCR index (cutoff = 362.48 µg/g) had a sensitivity of 78.6% and a specificity of 68.7% for identifying osteoporosis, with an area under the ROC curve of 0.760. CONCLUSIONS: These results suggest that UBCR levels correlate negatively with lumbar spine BMD and T and Z values and may serve as a marker for osteoporosis in Chinese elderly male adults.

6.
J Immunol Res ; 2020: 1480281, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32411786

RESUMO

Background: In situ vaccination-induced local inflammatory response resulted in the establishment of a pool of tissue-resident memory T (TRM) cells and new vessels after the resolution of inflammation. TRM cells have received increasing attention; however, the role of new vessels in protective response is still unknown. Materials and Methods: We performed the laparotomy to access the stomach and injected alum-based vaccine into the gastric subserous layer (GSL). At 28 days post vaccination, a parabiosis mouse model along with depletion of anti-CD90.2 antibody was employed to explore the function of perivascular lymphocyte clusters in recall responses. The composition of the gastric lymphocyte clusters was analyzed by immunofluorescence staining. Antibody responses were detected using ELISA. Gastric lymphocytes were analyzed using flow cytometry. Results: GSL vaccination induced the formation of new vessels in the inflamed region. These new vessels were different from native vessels in that they were generally accompanied by perivascular lymphocyte clusters that mainly consisted of CD90-expressing cells. Additionally, histological analysis revealed the presence of CD4+ and CD8+ T cells in the perivascular lymphocyte clusters. Administration of a dose of an anti-CD90.2 antibody to GSL-vaccinated mice resolved these clusters. The efficacy of protection was compared in the parabiosis mice. Upon challenge, the presence of perivascular lymphocyte clusters was responsible for the fast recall response, as depletion of these clusters by CD90.2 antibody administration resulted in decreased expressions of VCAM-1, Madcam-1, and TNF-α, as well as lower recruitment of proinflammatory immune cells, decreased antibody levels, and poor protection. Conclusions: Our research demonstrates that in situ vaccination-induced regional inflammatory response contributes to optimal recall response not only by establishing a CD4+ TRM pool but also by creating an "expressway," i.e., perivascular lymphocyte cluster.

7.
Environ Pollut ; 263(Pt A): 114414, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32244158

RESUMO

The trace elements contamination of agricultural soils near petrochemical industry complexes is a concern due to the risk of accumulating in food systems and subsequently affecting human health. We measured representative trace elements (Cu, Ni, Cr, Pb, Zn, Pb, Hg and As) through the soil-plant (maize)-human contamination pathway near a petrochemical industry complexes in an agricultural region from September 20 to 28, 2016. We found that the soil was mildly to moderately polluted by multiple trace elements, which was also confirmed by the contamination factor and enrichment factor values. Cd (enrichment factor = 2.28), Cu (2.75), Zn (1.85) and Pb (1.70) should be given more attention and prioritized over the other trace elements due to their higher potential risks. Furthermore, the trace elements contamination in maize grains was lower than the corresponding limits. The sequence of the transfer coefficient values was Zn > Cd > Cu > Hg > Ni > As > Cr > Pb. Maize grain safety was threatened mainly by Zn, Cd and Cu. There was no risk to humans through soil ingestion, while a potential health risk from maize grain consumption existed. Children were more sensitive than adults to the non-carcinogenic risks of maize grain consumption. Trace element As was found to be the priority metal for risk control. For carcinogenic risk, adults were more sensitive than children; As, Cr and Cd were the priority metals for risk control, with CRmaize values exceeding the risk threshold (1 × 10-4). Overall, strict, intensive monitoring, especially of Cr and Cd, and soil protection measures are needed to prevent any furthertrace elements contamination and to ensure food safety. This study also provides a reference for similar studies worldwide.


Assuntos
Metais Pesados/análise , Poluentes do Solo/análise , Oligoelementos/análise , Adulto , Criança , China , Monitoramento Ambiental , Humanos , Medição de Risco , Solo , Zea mays
8.
BMC Plant Biol ; 20(1): 141, 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32252634

RESUMO

BACKGROUND: Hypoxia causes injury and yield loss. Soil aeration has been reported to accelerate the growth of plants and increase crop yield. The aim of this study was to examine growth response of greenhouse-produced muskmelon to 3 levels of sub-surface drip irrigation (I), 3 different installation depths of drip laterals in the soil (D), and 4 levels of supplemental soil aeration frequency (A). A fractional factorial experiment was designed to examine these treatment effects on marketable fresh fruit yield, leaf area index during 3 growth stages, and dry matter partitioning at harvest. In addition, we studied the response of fruit yield and dry matter of tomato to 2 levels of burial depths of subsurface tubing in combination with 3 frequency levels of soil aeration. RESULTS: Results showed that soil aeration can positively influence the yield, leaf area index, dry matter and irrigation use efficiency of the muskmelon (p < 0.05). The fruit yield of muskmelon and tomato were increased by 21.5 and 30.8% respectively with 1-d and 2-d aeration intervals compared with the no aeration treatment. CONCLUSIONS: The results suggest that soil aeration can positively impact the plant root zone environment and more benefits can be obtained with aeration for both muskmelon and tomato plants.

9.
Biosci Rep ; 40(3)2020 03 27.
Artigo em Inglês | MEDLINE | ID: mdl-32159214

RESUMO

Astragalus polysaccharide (APS), a natural antioxidant found in Astragalus membranaceus emerging as a novel anticancer agent, exerts antiproliferative and pro-apoptotic activity in various cancer cell types, but its effect on ovarian cancer (OC) remains unknown. In the present study, we tried to elucidate the role and mechanism of APS in OC cells. Our results showed that APS treatment suppressed the proliferation and induced apoptosis in OC cells. Afterward, the microRNA (miRNA) profiles in APS-treated cells were determined by a microarray assay, and whether APS affected OV-90 cells through regulation of miRNA was determined. Among these aberrant miRNAs, miR-27a was selected for further study as its oncogenic roles in various human cancers. Moreover, we found overexpression of miR-27a reversed the antiproliferation and pro-apoptotic effects of APS on OC cells. F-box and WD-40 domain protein 7 (FBXW7), a classical tumor suppressor, was found directly targeted by miR-27a and its translation was suppressed by miR-27a in OC cells. Finally, it was also observed that knockdown of FBXW7 by si-FBXW7 reversed the tumor suppressive activity of APS in OC cells, which is similar to the effects of miR-27a overexpression. Our findings demonstrate that APS can suppress OC cell growth in vitro via miR-27a/FBXW7 axis, and this observation reveals the therapeutic potential of APS for treatment of OC.

10.
Environ Sci Technol ; 54(7): 3793-3802, 2020 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-32119771

RESUMO

Previous studies have estimated power plant cooling water consumption based on the long-term average cooling water consumption intensity (WI: water consumption per unit of electricity generation) at an annual scale. However, the impacts of the seasonality of WI and streamflow on electricity generation are less well understood. In this study, a risk assessment method is developed to explore the seasonal risk of water-electricity nexus based on the Integrated Environmental Control Model, which can simulate variable WIs in response to daily weather conditions and avoid underestimation in WIs as well as nexus risk during dry seasons. Three indicators, reliability, maximum time to recovery, and total power generation loss, are proposed to quantify the seasonal nexus risk under water consumption policy constraint represented by the allowed maximum percentage of water consumption to streamflow. The applications of the method in two representative watersheds demonstrate that the nexus risk is highly seasonal and is greatly impacted by the seasonal variability of streamflow rather than annual average water resources conditions on which most previous studies are based. The nexus is found more risky in the watershed with almost double mean annual streamflow and greater streamflow variability, compared with the watershed with less streamflow variability.


Assuntos
Ingestão de Líquidos , Eletricidade , Reprodutibilidade dos Testes , Medição de Risco , Estações do Ano
11.
Waste Manag ; 106: 145-154, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32217443

RESUMO

Crushing is the key part for the recycling technology of waste printed circuit boards (WPCBs). In this study, the breakage and liberation effects of WPCBs was improved by heat pretreatment technology before crushing (HPBC). Based on the test results, 200 °C was found as the safe temperature for the HPBC of WPCBs. The fracture mode, particle size distribution, and enrichment characteristics of WPCBs were systematically studied. The experimental results show that the HPBC changed the breakage mode from longitudinal fracture to horizontal fracture and improved the liberation of metal from non-metal components. During the crushing process, the increase in the heat pretreatment time (30-120 min) and temperature (100-200 °C) can improve the crushing effect of WPCBs and increase the content of crushing products of -0.3 mm by 3.16% and 5.64%, respectively. Compared to the non-metallic components, the metal components have ductility and are difficult to break into -0.3 mm during the crushing process. HPBC can promote copper enrichment to narrow grain size. In the 0.3-1 mm range, the content of copper increased from 47.87% to 57.61%, an increase by 9.74%. The initial enrichment of copper was achieved by adjusting the crushing time. The recovery rate of copper can reach 85.66%, and the enrichment rate is 1.74 when 0.3-2 mm breaking product is used as enrichment. Therefore, HPBC can effectively improve the crushing and liberation effect of WPCBs and improve the enrichment rate, and thus is an effective pretreatment method.


Assuntos
Resíduo Eletrônico , Cobre , Calefação , Metais , Reciclagem
12.
Biomed Res Int ; 2020: 1953242, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32051823

RESUMO

Brucella-caused brucellosis is one of the most widespread worldwide zoonoses. Lipopolysaccharide (LPS) of Brucella, which functions as pathogen-associated molecular patterns (PAMPs), is an important virulence factor that elicits protective antibodies. Per of B. melitensis is involved in the biosynthesis of the O-side chain of LPS. Autophagy is a crucial element of the innate immune response against intracellular pathogens including Brucella. In this study, we observed that autophagy was inhibited in RAW264.7 cells infected with Brucella melitensis ∆per. And, a high-throughput array-based screen and qRT-PCR validation were performed to identify the differentially expressed miRNAs in RAW264.7 cells infected with B. melitensis M5-90 ∆per. The results suggested that mmu-miR-146a-5p, mmu-miR-155-5p, mmu-miR-146b-5p, and mmu-miR-3473a were upregulated and mmu-miR-30c-5p was downregulated. During B. melitensis M5-90 ∆per infection, the increased expression of miR-146b-5p inhibited the autophagy activation in RAW264.7 cells. Using a bioinformatics approach, Tbc1d14 was predicted to be a potential target of miR-146b-5p. The results of a luciferase reporter assay indicated that miR-146b-5p directly targeted the 3'-UTR of Tbc1d14, and the interaction between miR-146b-5p and the 3'-UTR of Tbc1d14 was sequence-specific. High-throughput RNA-Seq-based screening was performed to identify differentially expressed genes in Tbc1d14-expressing RAW264.7 cells, and these were validated by qRT-PCR. Among the differentially expressed genes, four autophagy associated genes, IFNγ-inducible p47 GTPase 1 (IIGP1), nuclear receptor binding protein 2 (Nrbp2), transformation related protein 53 inducible nuclear protein 1 (Trp53inp1), and immunity-related GTPase family M member 1 (Irgm1), were obtained. Our findings provide important insights into the functional mechanism of LPS of B. melitensis.

13.
Dalton Trans ; 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31971192

RESUMO

Increasing the voltage window of an electrode material is effective for improving the energy density of aqueous symmetric supercapacitors. Herein, a novel aqueous symmetric supercapacitor equipped with a high cell voltage window of 2.6 V was assembled by P-doped TiO2 nanotube arrays on a Ti sheet. The arrays exhibit a wide potential range of about 1.2 V as the cathode, and a stable wide potential range of 1.4 V as the anode was also obtained. These wide potential windows in the cathode and anode render the symmetric supercapacitor with a very large working voltage window reaching 2.6 V, and thus a high volumetric energy density (1.65 mW h cm-3). These results suggest that P-doped TiO2 nanotube arrays can be promising candidates for energy storage devices.

14.
Biomed Pharmacother ; 122: 109696, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31918270

RESUMO

BACKGROUND: Dysregulation of miRNAs is associated with aberrant migration and invasion by suppressing relevant target genes in multiple cancers, including oral squamous cell carcinoma (OSCC). Accumulating evidence suggests that microRNA-155-5p is involved in carcinogenesis and tumor progression. However, the exact function and molecular mechanism of miR-155-5p in OSCC remain unclear. This study aimed to investigate the function of miR-155-5p and the molecular mechanisms underlying the influencing progression of OSCC. METHODS: The miR-155-5p expression level in the OSCC tissues and oral cancer cell lines were determined by the qRT-PCR. Gain-of-function and knockdown approach were used to examine the effect of miR-155-5p on cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of OSCC. The luciferase reporter assay was applied to confirm the AT-rich interactive domain 2 (ARID2) as a potential target of miR-155-5p, and the rescue experiment was employed to verify the roles of the miRNA-155-5p-ARID2 axis in OSCC progression. Immunohistochemical staining was used to detect ARID2 expression in another cohort sample tissues from OSCC patients. RESULTS: MiR-155-5p was significantly upregulated in OSCC tissues and cell lines. The miR-155-5p expression level was positively correlated with tumor size, TNM stage, histological grade and lymph node metastasis of OSCC patients. Functional assays demonstrated that miR-155-5p enhanced OSCC cell proliferation, migration and invasion. Mechanistically, ARID2 was identified as a direct target and functional effector of miR-155-5p in OSCC. Furthermore, ARID2 overexpression could rescue the aberrant biological function by overexpressed miR-155-5p in OSCC cells. Notably, we showed that ARID2 could be used as an independent prognosis factor in OSCC. CONCLUSIONS: Our results suggest that miR-155-5p facilitates tumor progression of OSCC by targeting ARID2, and miR-155-5p-ARID2 axis may be a potential therapeutic target of OSCC.

15.
J Colloid Interface Sci ; 565: 458-464, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31982712

RESUMO

As a promising anode material for supercapacitors, Fe2O3 has been widely studied but still face the problem of low conductivity. Inducing oxygen vacancy (Vo) into Fe2O3 is a widely used approach to tune the conductivity to enhance its capacitive performance, but there is little research on the influence of Vo content. Herein, we report the effect of Vo in Fe2O3 nanoplates with various content. We tuned the Vo content by annealing at 200-500 °C. XPS and EPR were taken to characterize the Vo content, ranging from 11% to 26%. Electrochemical results show that FO-300 with 17% Vo has the highest capacity of 301 mAh g-1, and the capacity of the highest Vo content's (26% Vo) is only 107 mAh g-1. The symmetric supercapacitor based on FO-300 shows a considerably high energy density of 58.5 Wh kg-1 at a power density of 9.32 kW kg-1 and remains 84.6% after 12,000 cycles.

16.
Viruses ; 12(1)2020 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-31963559

RESUMO

Orf is a zoonotic disease that has caused huge economic losses globally. Systematical analysis of dysregulated cellular micro RNAs (miRNAs) in response to Orf virus (ORFV) infection has not been reported. In the current study, miRNA sequencing and RNA sequencing (RNA-seq) were performed in goat skin fibroblast (GSF) cells at 0, 18, and 30 h post infection (h.p.i). We identified 140 and 221 differentially expressed (DE) miRNAs at 18 and 30 h.p.i, respectively. We also identified 729 and 3961 DE genes (DEGs) at 18 and 30 h.p.i, respectively. GO enrichment analysis indicates enrichment of apoptotic regulation, defense response to virus, immune response, and inflammatory response at both time points. DE miRNAs and DEGs with reverse expression were used to construct miRNA-gene networks. Seven DE miRNAs and seven DEGs related to "negative regulation of viral genome replication" were identified. These were validated by RT-qPCR. Cfa-let-7a, a significantly upregulated miRNA, was found to repress Thrombospondin 1 (THBS1) mRNA and protein expression by directly targeting the THBS1 3' untranslated region. THBS1 has been reported to induce apoptosis; therefore, the cfa-let-7a-THBS1 axis may play an important role in cellular apoptosis during ORFV infection. This study provides new insights into ORFV and host cell interaction mechanisms.

17.
Mol Carcinog ; 59(4): 353-364, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31995261

RESUMO

Despite therapeutic advancements, there has been little improvement in the survival status of patients with oral squamous cell carcinoma (OSCC). HOX antisense intergenic RNA (HOTAIR) has been shown to be dysregulated in several cancer types. However, the roles of HOTAIR in OSCC remain largely unknown. In this study, we investigated the association of HOTAIR expression with clinicopathological features in OSCC patients and the crucial roles of HOTAIR in the modulation of tumor progression. Our results showed that HOTAIR was highly expressed both in OSCC tissue samples and cell lines compared with corresponding normal oral mucosa tissues and human oral keratinocytes. Its overexpression was positively correlated with TNM (tumor-node-metastases) stage, histological grade, and regional lymph node metastasis. The knockdown of HOTAIR by short hairpin RNA significantly decreased the migration, invasion, and epithelial-mesenchymal transition of OSCC cells in vitro. Moreover, there was a negative correlation between HOTAIR and microRNA-326 expression in OSCC tissue samples and cell lines. Luciferase reporter and loss-of-function assays revealed that HOTAIR acted as a competitive endogenous RNA effectively sponging miR-326, thereby regulating the derepression of metastasis-associated gene 2 (MTA2). Finally, the expression and clinical significance of MTA2 were analyzed in another cohort of OSCC tissue samples. High MTA2 expression was significantly correlated with clinicopathological features of advanced OSCC and poor prognosis for patients with OSCC. Collectively, HOTAIR overexpression promoted the progression of OSCC. The HOTAIR-miR-326-MTA2 axis may contribute to a better understanding of OSCC pathogenesis and be a potential therapeutic target for OSCC.


Assuntos
Carcinoma de Células Escamosas/genética , Histona Desacetilases/genética , Neoplasias Bucais/genética , RNA Longo não Codificante/genética , Proteínas Repressoras/genética , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Linhagem Celular , Linhagem Celular Tumoral , Intervalo Livre de Doença , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Histona Desacetilases/metabolismo , Humanos , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , Neoplasias Bucais/metabolismo , Neoplasias Bucais/patologia , Invasividade Neoplásica , Metástase Neoplásica , Interferência de RNA , Proteínas Repressoras/metabolismo
18.
World Neurosurg ; 134: e243-e248, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31629147

RESUMO

BACKGROUND: Selection of approach for subaxial cervical fracture-dislocation (SCFD) is controversial. The questions of whether a posterior ligamentous structure (PLS) can be functionally healed in patients with SCFD and how long this healing process takes are critical in these patients. METHODS: This study retrospectively enrolled 394 patients with SCFD who underwent anterior decompression, reduction, and fusion between January 2002 and December 2017. The definition of functional healing of PLS was based on evaluations of clinical function and radiographic stability of PLS. RESULTS: Follow-up was available for 354 patients (89.8%). The PLS of 339 cases was functionally healed at the first follow-up interval without any posterior surgical intervention. No hardware failure or progressive cervical kyphosis was observed at further follow-up. At 12 months postoperatively, interbody fusion was satisfactory. However, the other 15 patients experienced nonhealing PLS at 8 weeks postoperatively and developed cervical deformity at further follow-up. Five patients refused revision surgery; the other 10 patients obtained solid fusion after revision surgeries. CONCLUSIONS: Among 354 patients with SCFD and treated by single anterior reduction and fixation, simple PLS injury without any bony instability at the posterior column of the cervical spine can be functionally healed in 95.8% of patients by external fixation for 8 weeks. Whether this finding is applicable to various types of SCFD should be verified in further prospective studies with larger samples.


Assuntos
Vértebras Cervicais/cirurgia , Luxações Articulares/cirurgia , Procedimentos Cirúrgicos Reconstrutivos/tendências , Fraturas da Coluna Vertebral/cirurgia , Fusão Vertebral/tendências , Adulto , Vértebras Cervicais/diagnóstico por imagem , Feminino , Seguimentos , Humanos , Luxações Articulares/diagnóstico por imagem , Imagem por Ressonância Magnética/tendências , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fraturas da Coluna Vertebral/diagnóstico por imagem
19.
Sci Total Environ ; 706: 135729, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31836224

RESUMO

Environmental flow science has attracted significant attention in recent years and has become more widely implemented by various agencies responsible for managing water resources. In addition to annual environmental flows, monthly environmental flows have been increasingly used to protect aquatic ecosystems. Regional regression analysis is commonly used to estimate streamflows when long-term continuous streamflow records are not available. While literature related to regional regression models for estimating annual flows is relatively rich, studies focused on regional regression analysis for estimating monthly flows are rare. This study contributes a comprehensive assessment of regional regression models for estimating monthly flows. A comprehensive database of watershed characteristics was developed, and a suite of monthly and annual flows were estimated using long-term continuous streamflow records from 72 watersheds within or adjacent to the Susquehanna River Basin. Regional regression models were developed for 104 flows, including 96 monthly flow statistics and 8 annual flow statistics. The performance of regional regression models for estimating various monthly flows, as well as which watershed characteristics were most important for estimating them, was investigated. The results showed regional regression analysis performed better for wet months than dry months, and better for high and medium flows than low flows. Drainage area and precipitation were the most important watershed characteristics for estimating flows. The performance of regional regression models for estimating annual flow was often better for estimating monthly flows of dry months but worse than for estimating monthly flows of wet months. The study also provides guidance for water resources managers regarding where to focus streamflow monitoring efforts with limited resources, considering flow estimation error is greatest for low flow statistics in dry weather months.

20.
J Colloid Interface Sci ; 560: 447-457, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31677818

RESUMO

It has been demonstrated that the incorporation of Ni into metal oxide-based gas sensors often enhances the sensing performance by increasing the catalytic and heterojunction effects. However, it remains unclear how these two effects work either individually or synergistically in gas sensing. Herein, a series of Ni-doped In2O3 nanotubes (NIO NTs) with different doping concentrations were synthesized through a traditional electrospinning technique. The as-prepared NIO NTs were uniform, with length of micron scale, an average diameter of approximately 70 nm, and a tube wall thickness of approximately 10 nm. Following their incorporation into gas sensors, the NIO NTs often showed improved sensing properties (including excellent response and selectivity) for ethanol vapor compared to the pristine In2O3 NTs. Specifically, at 100 ppm ethanol and 220 °C, the response of NIO-7 NTs (7 mol% Ni) was approximately four-fold higher than that of pristine In2O3 NTs (49.74 vs. 13.39). The gas sensing test results indicated that the improved sensing performance was due to the formation of a heterojunction between In2O3 and NiO, as well as to the catalysis effects of Ni3+ ions. Additionally, simulation results indicated that the improved gas selectivity could be due to the Ni doping-induced change in surface adsorption energies of the tested gases.

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