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1.
Food Chem ; 366: 130575, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34293546

RESUMO

Two bimetallic CoNi-based metallo-covalent organic frameworks (MCOFs) were prepared and explored as the sensitive platforms of impedimetric aptasensors for efficient detection of tobramycin (TOB). The two CoNi-MCOFs were constructed using metallophthalocyanine tetra-amine (MPc-TA, M = Co2+ or Ni2+) and 4,4'-(1,10-phen-anthroline-2,9-diyl) dibenzaldehyde (PTD) as building units and further coordinating to the secondary metal ions (Ni2+ or Co2+) by phenanthroline. Interestingly, the immobilization ability of CoPc-TA-PTD(Ni) to TOB-targeted aptamer is higher than that of NiPc-TA-PTD(Co) due to its stronger binding interactions to aptamer. As a result, the CoPc-TA-PTD(Ni)-based aptasensor shows the superior TOB detection ability, giving a low detection limit of 0.07 fg mL-1 and satisfied sensing performances, such as high selectivity, good reproducibility, and excellent stability. Also, the aptasensor shows the acceptable applicability for detecting TOB in milk or chicken egg. This MCOFs-based sensing strategy could be extensively applied to detect other analytes by anchoring the corresponding probes.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Estruturas Metalorgânicas , Técnicas Eletroquímicas , Limite de Detecção , Reprodutibilidade dos Testes , Tobramicina
3.
ACS Chem Neurosci ; 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34637270

RESUMO

C5a receptor 1 (C5aR1) can induce a strong inflammatory response to an injury. Targeting C5aR1 has emerged as a novel anti-inflammatory therapeutic method. However, the role of C5aR1 in cerebral ischemia and reperfusion (I/R) injury and the definitive mechanism have not been elucidated clearly. Here, we determined whether C5aR1 signaling was essential to the post-ischemic inflammation and brain injury and whether it is a valid target for therapeutic blockade by using soluble receptor antagonist PMX53 in the early stage after I/R injury. In an in vitro model (oxygen and glucose deprivation and reperfusion, OGD/R) and in vivo model (middle cerebral artery occlusion and reperfusion, MCAO/R) of I/R, the neuronal cells of rats showed significantly up-regulated gene expression of C5aR1, and a notable inflammatory response was demonstrated with elevated tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), and IL-6. Inhibition of C5aR1 by PMX53 treatment significantly reduced cell injury and inflammation and promoted brain function recovery. Further mechanism studies showed that inhibiting C5aR1 by PMX53 protected the rats from MCAO/R injury, decreased cell inflammation, and apoptosis via inhibiting the TLR4 and NF-κB signaling pathway and reducing the production of TNF-α, IL-1ß, and IL-6 in MCAO/R rats. In addition, manipulation of the C5aR1 gene expression in vitro displayed that the inflammatory cascade signals including TLR4, TNF-α, IL-1ß, and IL-6 were coincidently regulated with the regulation of C5aR1 expression levels. Thus, our results demonstrated a pathogenic role for C5aR1 in the progression of brain injury and inflammation response following I/R injury. Our study clearly demonstrated that C5aR1 inhibition might be an effective treatment strategy for ischemic stroke.

4.
BMC Med ; 19(1): 243, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34641873

RESUMO

BACKGROUND: Plasma cell-free DNA (cfDNA) methylation has shown promising results in the early detection of multiple cancers recently. Here, we conducted a study to investigate the performance of cfDNA methylation in the early detection of esophageal cancer (ESCA). METHODS: Specific methylation markers for ESCA were identified and optimized based on esophageal tumor and paired adjacent tissues (n = 24). Age-matched participants with ESCA (n = 85), benign esophageal diseases (n = 10), and healthy controls (n = 125) were randomized into the training and test sets to develop a classifier to differentiate ESCA from healthy controls and benign esophageal disease. The classifier was further validated in an independent plasma cohort of ESCA patients (n = 83) and healthy controls (n = 98). RESULTS: In total, 921 differentially methylated regions (DMRs) between tumor and adjacent tissues were identified. The early detection classifier based on those DMRs was first developed and tested in plasma samples, discriminating ESCA patients from benign and healthy controls with a sensitivity of 76.2% (60.5-87.9%) and a specificity of 94.1% (85.7-98.4%) in the test set. The performance of the classifier was consistent irrespective of sex, age, and pathological diagnosis (P > 0.05). In the independent plasma validation cohort, similar performance was observed with a sensitivity of 74.7% (64.0-83.6%) and a specificity of 95.9% (89.9-98.9%). Sensitivity for stage 0-II was 58.8% (44.2-72.4%). CONCLUSION: We demonstrated that the cfDNA methylation patterns could distinguish ESCAs from healthy individuals and benign esophageal diseases with promising sensitivity and specificity. Further prospective evaluation of the classifier in the early detection of ESCAs in high-risk individuals is warranted.

5.
Lipids Health Dis ; 20(1): 131, 2021 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-34627270

RESUMO

BACKGROUND: Limited studies regarding the correlation of lipid accumulation product (LAP) with a decreased estimated glomerular filtration rate (eGFR) have yielded conflicting findings, and no report has demonstrated the relationship of LAP with chronic kidney disease (CKD), defined as the presence of albuminuria and/or a decreased eGFR. The purpose of this study was to estimate the possible correlation of LAP with CKD prevalence in Chinese community adults. METHOD: In this cross-sectional study, LAP level of 7202 participants (age ≥ 40 years) was determined, and its possible association with CKD was evaluated by a multiple logistic regression model. RESULTS: Compared with subjects with non-CKD, non-albuminuria, and high eGFR, LAP levels significantly increased in female not male subjects with CKD, albuminuria, and low eGFR, respectively (all P < 0.001). The univariate logistic regression analysis revealed that LAP level of female not male subjects were significantly and positively associated with the prevalence of CKD (P < 0.001). The multivariate logistic regression analysis showed that the risk of CKD prevalence in female not male subjects progressively increased across LAP quartiles (P for trend < 0.01), and the risk of CKD prevalence of subjects in Q4 significantly increased compared to those in Q1 after adjustment for potential confounding factors in Models 4 (odds ratio [OR]: 1.382, 95% confidence intervals [CI] 1.002-1.906, P < 0.05). Stratified analysis revealed positive associations of LAP quartiles with risk of CKD prevalence in people with the following characteristics: women, older, overweight, with hypertension, normal glucose tolerance, appropriate low-density lipoprotein cholesterol, nonsmokers, nondrinkers, and no cardiovascular disease events. CONCLUSIONS: High LAP levels might be significantly associated with risk of CKD prevalence in community-dwelling Chinese female adults, which may inform both public health recommendations and clinical practice.

6.
Food Funct ; 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34607339

RESUMO

P2X7 receptor (P2X7R) and NLRP3 cooperatively participate in inflammation and hepatocyte damage during hepatic injury induced by lipopolysaccharides (LPS). High-mobility group box 1 (HMGB1) released from immune cells in response to such stimuli plays a vital role in mediating inflammation via TLR4 and the receptor for advanced glycation end products (RAGE), a receptor for HMGB1. However, the correlation among P2X7R, RAGE and TLR4 in regulating the release of HMGB1 has not been elucidated. Increasing the number of daily foods is found to be beneficial for hepatocyte damage in septic hepatic injury. Hence, we investigated the effects of luteolin, a natural flavonoid mainly existing in vegetables and fruits, on liver injury, focusing on how luteolin participates in hepatitis based on the P2X7R-RAGE-TLR4 axis by regulating the release of HMGB1. The results demonstrated that the indicators of hepatic injury such as increased ALT, AST in the serum and infiltration of immune cells were attenuated after luteolin treatment in LPS-induced mice. Luteolin could also suppress the production and release of HMGB1 and the activation of caspase 1 both in LPS-induced mice and LPS/ATP-stimulated HepG2 cells. Collectively, luteolin reversed LPS-induced hepatic injury, especially inflammation, likely by regulating the release of HMGB1 through the P2X7R-RAGE-TLR4 axis.

7.
Plant Dis ; 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34645299

RESUMO

Strawberry (Fragaria × ananassa) is widely cultivated in Liaoning Province, the most important strawberry production area in China. In November 2020, extensive brown spotting on the calyces and receptacles and dark brown necrotic lesions on the leaves of 'YanLi' strawberry plants were observed in the greenhouse, College of Horticulture, in Shenyang Agriculture University of Liaoning Province, China. The incidence ranged from 15% to 20% in the greenhouse. As the disease progressed, the spots extended to cover the entire receptacles and entire leaves. Fresh samples (5 mm2) were cut from the margin of healthy and infected tissues. The isolation and purification of methods were as described previously (Obregón et al. 2018). Fungal isolations are suborbicular with irregular edges. The colonies showed white aerial mycelium at the early stage and formed concentric circles after 5 days culture at 28°C on PDA. Black viscous acervuli appeared and scattered on the surface of the colony after 7~10 days culture. Acervulus was 162.4 µm in average diameter, n= 30. Conidia were spindle shape, five cells, four septa, 7.0 µm (average width) and 24.0 µm (average length), n=30. The apical and basal cells are lighter in color, most of them are hyaline, the middle three cells are darker in color, mostly brown. There are 2 to 4 colorless and transparent unbranched accessory filaments at the top, 30.8 µm in average length, and the basal cell has a small appendage, 9.0 µm in average length, n=30 (Fig. 1c-g). For fungal identification to species level, genomic DNA was extracted by the 2% CTAB method. Internal transcribed spacer (ITS) region, ß-tubulin gene (TUB2) and translation elongation factor-1α (TEF-1α) were amplified (Qiu et al. 2020), respectively. Purified PCR products were sequenced in single direction. BLASTn searches of GenBank, using the partial ITS (MW861730), ß-tubulin (MW856302) and TEF-1α (MW861729) sequences of representative isolate NP102 as the queries, revealed 97%, 100% and 100% sequence identity, respectively, to Neopestalotiopsis clavispora strain WJ31 accessions MG729690, MG740736 and MG740759. The phylogenetic tree was constructed by MEGA X (Fig. 2) (Kumar et al. 2018). Pathogenicity assay was carried out by spraying a spore suspension (1 × 106 spores/ml) of 8-day-old culture of fungus on six healthy 'YanLi' strawberry plants. An additional six control plants were only sprayed with sterile distilled water, and the inoculated plants were maintained in a light incubator at 25 ± 1°C, 90% relative humidity, with a photoperiod of 12 h. Calyx and receptacle blight and leaf spot symptoms resembled those observed in the greenhouse on all inoculated tissues after 10 days (Fig. 1h and i), whereas calyces, receptacles and leaves on control plants remained asymptomatic. The experiment was repeated twice. Neopestalotiopsis clavispora was successfully reisolated from the infected tissues, fulfilling Koch's postulates. Researchers in Spain (Chamorro et al. 2016) reported N. clavispora for the first time on strawberry. After two years, N. clavispora was found in Argentina (Obregón et al. 2018). The reports characterized it mainly as a root rot pathogen. In January 2021, Neopestalotiopsis spp. were reported that it is a pathogen of strawberry leaves and fruits in Florida strawberry fields (Juliana et al. 2021). Our studies indicated that isolate NP102 was more aggressive. In the warm and humid greenhouse, the disease struck suddenly at the early stages of strawberry fruiting. Unlike previous reports, symptoms were a calyx and receptacle blight as well as a leaf spot. This pathogen can cause serious economic losses to strawberry growers. To the best of our knowledge, this is the first report of calyx and receptacle blight by N. clavispora on strawberry in China.

8.
Dalton Trans ; 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34553722

RESUMO

A photoelectrochemical (PEC)-electrochemical (EC) dual-mode biosensing strategy based on COF@MOF heterostructure was developed for efficiently analyzing Cr(III) ions. A two-dimensional phthalocyanine-based COF (CoPc-PT-COF) was in situ grown on a Cu-based MOF (Cu-MOF) substrate via covalent binding between carboxyl groups in Cu-MOF and amino groups in CoPc-PT-COF (denoted as CoPc-PT-COF@Cu-MOF). The coexistence of both phthalocyanine and bipyridine in CoPc-PT-COF@Cu-MOF affords the outperformed electro- and photo-activities, thus serving as a photoelectric beacon with favorable energy-band configuration and amplified electrochemical response. Due to the high porosity and rich functionality of the obtained heterostructure, the DNA strands can be tightly anchored over CoPc-PT-COF@Cu-MOF via diverse interactions. Thanks to the specific recognition between DNA strands and Cr3+ ions, the CoPc-PT-COF@Cu-MOF-based biosensor can be used to determine Cr3+ ions in an aqueous environment by PEC-EC mode. The gained biosensor shows an extremely low limit of detection (LOD) of 14.5 fM (for PEC) and 22.9 fM (for EC) within the Cr3+ concentration range from 0.1 pM to 100 nM, along with high selectivity, good reproducibility and stability. Moreover, this novel biosensor exhibits acceptable applicability for analyzing the trace Cr3+ from diverse samples (e.g., river and tap water). As a result, this work provides new insights into the construction of a high-efficiency PEC-EC dual-mode biosensor for detecting heavy metal ions from complex environments.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34556457

RESUMO

OBJECTIVE: Bone volume fraction (BV/TV) was used as a quantitative indicator of bone mineral density (BMD) in the posterior dental implant area, and the relationship between it and the initial stability of the implant was analyzed. METHODS: 53 patients with posterior dental implantation were selected. Remove the bone fragments from the planting area and put them in the formalin solution. Then, the implant was placed after the operator prepared the hole step by step. Peak Insertion torque value (ITV) and Implant stability quotient (ISQ) were recorded. The BV/TV of the bone fragments was measured by Image pro Plus6.0. The correlation between it and ITV and ISQ was analyzed. RESULTS: BV/TV was correlated with ITV and ISQ in the posterior dental planting area (r=0.862, r=0.832, P < 0.05). The correlation of the parameters in the posterior part of maxilla was higher than that in the mandible. There were significant differences in BV/TV, ITV, and ISQ between the mandible and maxilla, and the implant area of the mandible was higher than that of the maxilla. CONCLUSION: BV/TV can provide an objective index for BMD in the implant area, which has an important influence on the evaluation of the initial stability of the implant.

10.
Medicine (Baltimore) ; 100(35): e27058, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34477137

RESUMO

ABSTRACT: The treatment for squamous cell lung cancer (SqCLC) is limited, and the prognosis of SqCLC is poor. In this article, we aimed to analyze and identify immune-related cells and competition endogenous RNA (ceRNA) that influence the prognosis of SqCLC. SqCLC and lung adenocarcinoma data were downloaded from TCGA-GDC. A total of 22 types of immune cell fractions were estimated using CIBERSORT. R software was used to identify any significantly different transcriptome data, including mRNA, LncRNA, and miRNA. The univariate cox regression method was applied to screen for prognosis-related lncRNA, miRNA, mRNA and tumor-infiltrating immune cells. There were 504 patients included in this study. There was a higher proportion of memory activated CD4+ T cells and CD8+ T cells in younger women. Follicular helper T (Tfh) cells were predictive of a good prognosis and reflected immune activation in SqCLC. The SFTA1P/NKX2-1-AS1, hsa-mir-503, GREM2 ceRNA axes and NKX2-1-AS1, hsa-mir-96, PROK2 ceRNA axes were found to be important for the immune function, pathogenesis, and prognosis of SqCLC. Collectively, the immune-related ceRNA and tumor-infiltrating immune cells in SqCLC are likely important determinants of SqCLC pathogenesis, prognosis, and immune status.


Assuntos
Neoplasias Pulmonares/imunologia , Neoplasias de Células Escamosas/imunologia , Adolescente , Idoso , Criança , Células Epiteliais/patologia , Feminino , Ontologia Genética , Humanos , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Neoplasias de Células Escamosas/patologia , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos
11.
Mikrochim Acta ; 188(10): 316, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34476615

RESUMO

A novel label-free surface plasmon resonance (SPR) aptasensor has been constructed for the detection of N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots (Nb2C-SH QDs) as the bioplatform for anchoring N-gene-targeted aptamer. In the presence of SARS-CoV-2 N-gene, the immobilized aptamer strands changed their conformation to specifically bind with N-gene. It thus increased the contact area or enlarged the distance between aptamer and the SPR chip, resulting in a change of the SPR signal irradiated by the laser (He-Ne) with the wavelength (λ) of 633 nm. Nb2C QDs were derived from Nb2C MXene nanosheets via a solvothermal method, followed by functionalization with octadecanethiol through a self-assembling method. Subsequently, the gold chip for SPR measurements was modified with Nb2C-SH QDs via covalent binding of the Au-S bond also by self-assembling interaction. Nb2C-SH QDs not only resulted in high bioaffinity toward aptamer but also enhanced the SPR response. Thus, the Nb2C-SH QD-based SPR aptasensor had low limit of detection (LOD) of 4.9 pg mL-1 toward N-gene within the concentration range 0.05 to 100 ng mL-1. The sensor also showed excellent selectivity in the presence of various respiratory viruses and proteins in human serum and high stability. Moreover, the Nb2C-SH QD-based SPR aptasensor displayed a vast practical application for the qualitative analysis of N-gene from different samples, including seawater, seafood, and human serum. Thus, this work can provide a deep insight into the construction of the aptasensor for detecting SARS-CoV-2 in complex environments. A novel label-free surface plasmon resonance aptasensor has been constructed to detect sensitively and selectively the N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots as the scaffold to anchor the N-gene-targeted aptamer.


Assuntos
Aptâmeros de Nucleotídeos , COVID-19/diagnóstico , Nióbio/química , Nucleocapsídeo/metabolismo , Pontos Quânticos/química , SARS-CoV-2/isolamento & purificação , Ressonância de Plasmônio de Superfície/métodos , COVID-19/virologia , Humanos , Limite de Detecção
12.
Compr Rev Food Sci Food Saf ; 20(5): 4367-4389, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34397139

RESUMO

Polysaccharides are natural polymer compounds widely distributed in plants, animals, and microorganisms, most of which have a broad spectrum of biological activities to promote human health. They could also be used as texture modifiers in food industry due to their excellent rheological and mechanical properties. Many researchers have shown that nonthermal processing technologies have numerous advantages, such as high extraction efficiency, short extraction time, and environmental friendliness, in the extraction of polysaccharides compared with the traditional extraction methods. Moreover, nonthermal technologies could effectively change the physicochemical properties and structural characteristics of polysaccharides to improve their biological activities or processing properties. Therefore, a comprehensive summary about the extraction and modification of polysaccharides by nonthermal technologies, including ultrasound, high hydrostatic pressure, pulsed electric fields, and cold plasma, was provided in this review. In particular, the underlying mechanisms, processing operations, and current application status of these technologies were discussed. In addition, the applications of combining nonthermal techniques with other technological methods in polysaccharide extraction and modification were briefly introduced.

13.
Lab Invest ; 101(11): 1458-1466, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34392309

RESUMO

Bone regeneration is a coordinated process involving connections between blood vessels and osteocytes. Angiogenesis and osteogenesis are tightly connected throughout the progression of bone regeneration. This study aimed to explore the underlying mechanism of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)-regulated angiogenesis during bone regeneration. Gene and protein expression was detected by quantitative real-time PCR and western blot assay. Vascular endothelial growth factor (VEGFA) secretion was assessed by enzyme-linked immunosorbent assay. To evaluate the effect of osteogenic differentiation, alkaline phosphatase (ALP) and alizarin red staining assays were performed. Proliferation was detected by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Migration and angiogenesis were measured using Transwell and tube formation assays. A dual luciferase reporter assay was performed to confirm the binding relationship among MALAT1, miR-494, and specificity protein 1 (SP1). Expression levels of MALAT1, SP1, and VEGFA were elevated and miR-494 was suppressed in MC3T3-E1 cells after culture in osteogenic medium. MALAT1 knockdown suppressed the osteogenic differentiation of MC3T3-E1, since ALP activity, mineralized nodules, and expression of the osteodifferentiated markers runt-related transcription factor 2 and osterix were restrained. In addition, MALAT1 silencing inhibited angiogenesis during bone regeneration, as the proliferation, migration, and capillary tube formation of human umbilical vein endothelial cells were blocked. Furthermore, miR-494 was directly targeted by MALAT1 and regulated the SP1/Toll-like receptor 2 (TLR2)/bone morphogenetic protein 2 (BMP2) axis by targeting SP1. Furthermore, miR-494 overexpression inhibited angiogenesis and osteogenic differentiation. Moreover, SP1 overexpression or miR-494 inhibition rescued the regulatory effect of sh-MALAT1 on angiogenesis and osteogenic differentiation. Taken together, these findings indicate that MALAT1 promotes angiogenesis and osteogenic differentiation by targeting miR-494 and activating the SP1/TLR2/BMP2 pathway, suggesting a novel target for bone regeneration therapy by promoting angiogenesis.

14.
Artigo em Inglês | MEDLINE | ID: mdl-34227001

RESUMO

The high-density distribution patterns of the nutrients (C, N, P) and heavy metals (Fe, Mg, Zn, Cr, Pb, Ni, Cu, Cd) in sediments from Lake Luoma, as well as their pollution status and ecological risks, were characterized, to comprehensively understand potential environmental impacts of inter-basin water transfers. TN, TP, and OM were measured from 162.50 to 4360.00 mg kg-1, 165.00 to 1302.50 mg kg-1, and 1% to 13%, which were primarily accumulated in the eastern, northwest, and western regions, respectively. A total of 8 heavy metals except for Fe generally exhibited a similar distribution pattern, reflected by a gradually decreasing trend from northwest to southeast region. The averaged concentrations of heavy metals decreased as follows: Fe > Mg > Zn > Cr > Pb > Ni > Cu > Cd. Comprehensive pollution risk assessments indicated that the sediments of Lake Luoma were heavily polluted by TN, OM, and Cd. Multivariate statistical analyses demonstrated that the main pollution sources of Zn and Ni were fertilizers and pesticides, Cd and Pb are mainly derived from industrial wastewater, and TN and OM may come from natural and agricultural factors. This research can provide data support for water pollution control and drinking water diversion management in the Lake Luoma basin.

15.
Int J Mol Sci ; 22(12)2021 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-34203082

RESUMO

Ideal Plant Architecture 1 (IPA1) encodes SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 14 (SPL14) with a pleiotropic effect on regulating rice development and biotic stress responses. To investigate the role of IPA1 in early seedling development, we developed a pair of IPA1/ipal-NILs and found that seed germination and early seedling growth were retarded in the ipa1-NIL. Analysis of the soluble sugar content, activity of amylase, and expression of the α-amylase genes revealed that the starch metabolism was weakened in the ipa1-NIL germinating seeds. Additionally, the content of bioactive gibberellin (GA) was significantly lower than that in the IPA1-NIL seeds at 48 h of imbibition. Meanwhile, the expression of GA synthesis-related gene OsGA20ox1 was downregulated, whereas the expression of GA inactivation-related genes was upregulated in ipa1-NIL seeds. In addition, the expression of OsWRKY51 and OsWRKY71 was significantly upregulated in ipa1-NIL seeds. Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of α-amylase genes. In summary, our results suggest that IPA1 negatively regulates seed germination and early seedling growth by interfering with starch metabolism via the GA and WRKY pathways.


Assuntos
Proteínas de Ligação a DNA/metabolismo , Giberelinas/metabolismo , Oryza/fisiologia , Desenvolvimento Vegetal , Plântula/crescimento & desenvolvimento , Transdução de Sinais , Amido/metabolismo , Fatores de Transcrição/metabolismo , Regulação da Expressão Gênica de Plantas , Germinação/genética , Fenótipo , Ligação Proteica , alfa-Amilases/metabolismo
16.
J Nanobiotechnology ; 19(1): 224, 2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34320999

RESUMO

Systemic chemotherapy is still the primary treatment for advanced-stage nasopharyngeal carcinoma (NPC), but only limited therapeutic success has been achieved in the past decade because of drug resistance and systemic toxicity. Curcumin (Cur) is an effective alternative to chemotherapeutics because it showed remarkable therapeutic potential in the treatment of NPC. However, lack of tissue specificity and poor penetration in solid tumors are the major obstacles to effective therapy. Therefore, in this work, a self-assembled sub-30 nm therapeutic lipid nanoparticle loaded with Cur, named as Cur@α-NTP-LN, was constructed, specifically targeting scavenger receptor class B member 1 (SR-B1) and enhancing its therapeutic effects on NPC in vivo. Our results showed that Cur@α-NTP-LNs were effective and superior to free Cur on NPC cell-specific targeting, suppressing cell proliferation and inducing cell apoptosis. In vivo and ex vivo optical imaging revealed that Cur@α-NTP-LNs exerted high targeting efficiency, specifically accumulating in NPC xenograft tumors and delivering Cur into the tumor center after systemic administration. Furthermore, Cur@α-NTP-LNs exhibited a remarkable inhibitory effect on the growth of NPC subcutaneous tumors, with over 71 and 47% inhibition compared to Cur- and α-NTP-LNs-treated groups, respectively. In addition, Cur@α-NTP-LNs almost blocked NPC metastasis in a lung metastasis model of NPC and significantly improved the survival rate. Thus, the sub-30 nm Cur@α-NTP-LNs enhanced the solubility of Cur and demonstrated the ability of targeted Cur delivery into the center of the solid NPC tumor, performing synergistic inhibitory effects on the growth of NPC tumor and its metastasis with high efficiency.

17.
Radiother Oncol ; 162: 34-44, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34214613

RESUMO

BACKGROUND AND PURPOSE: Radiotherapy (RT) has a promising anti-tumor effect depending on its effects on both cancer cells and tumor immune microenvironment (TIME). As one of the most common alterations in hepatocellular carcinoma (HCC), wnt/ß-catenin pathway activation, has been reported to induce radioresistance and suppressive TIME. In this study, we aim to explore the effect of wnt/ß-catenin inhibitor ICG-001 on radiosensitivity and RT-related TIME of HCC and the underlying mechanism. MATERIALS AND METHODS: C57BL/6 and nude mouse tumor models were used to evaluate the efficacy of different treatments on tumor growth, recurrence and mice survival. Flow cytometry was performed to assess tumor infiltrating lymphocytes (TILs). DNA damage response (DDR) and radioresistance was investigated by colony formation assays, γ-H2AX and micronuclei measurements. RESULTS: The addition of ICG-001 to RT exhibited better anti-tumor and survival-prolong efficacy in C57BL/6 than nude mice. TILs analysis revealed that ICG-001 plus RT boosted the infiltration and IFN-γ production of TIL CD8+ T cells, meanwhile reduced the number of Tregs. Moreover, mechanistic study demonstrated that ICG-001 increased the radiation-induced DDR of HCC cells by suppressing p53, thus leading to stronger activation of cGAS/STING pathway. Utilization of cGAS/STING pathway inhibitors impaired the therapeutic effect of combination therapy. Furthermore, combination therapy led to stronger immunologic memory and tumor relapse prevention. CONCLUSIONS: Our findings showed that ICG-001 displayed both local and systematic effects by increasing radiosensitivity and improving immunity in HCC, which indicated that ICG-001 might be a potential synergetic treatment for radiotherapy and radioimmunotherapy in HCC patients.

18.
Biosci Trends ; 15(3): 161-170, 2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34078766

RESUMO

This study aimed to investigate the value of multidisciplinary team (MDT) management in minimally invasive treatment of complex intrahepatic bile duct stones (IHDs) by laparoscopy, choledochoscopy and percutaneous choledochoscopy. The characteristics, perioperative index, complication rate and minimally invasive rate of patients in MDT group (n = 75) and non-MDT group (n = 70) were compared. The members of MDT include doctors in ultrasound, imaging, hepatobiliary and pancreatic surgery, anaesthesia and intensive care medicine. The results showed that minimally invasive surgery reduced the incidence of postoperative residual stones, OR (95% CI) = 0.365 (0.141-0.940) (p = 0.037). MDT reduced the operation time, OR (95% CI) = 0.406 (0.207-0.796) (p = 0.009). Minimally invasive surgery significantly reduced intraoperative bleeding, OR (95% CI) = 0.267 (0.133-0.534) (p < 0.001). Minimally invasive surgery also reduced hospitalization time, OR (95% CI) = 0.295 (0.142-0.611) (p = 0.001). The stone clearance rates of MDT group and non-MDT group were 81.33% and 81.43% respectively. In the MDT group, the operative time was less than that in the non-MDT group (p = 0.010); the intraoperative bleeding volume was significantly less than that in the non-MDT group (p < 0.001); the hospitalization time was less than that in the non-MDT group (p = 0.001). Minimally invasive operation rate:48 cases (64.00%) in MDT group were significantly higher than 17 cases (24.29%) in non-MDT group (p < 0.001). In conclusion, minimally invasive procedures can be selected more through MDT. MDT can shorten the operation time, and minimally invasive surgery can reduce the incidence of residual stones, reduce intraoperative bleeding, and may shorten hospital stay. Therefore, MDT management model can provide personalized and minimally invasive surgical protocol for patients with complex IHD, which has high application value.

19.
Adv Mater ; 33(29): e2006836, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34096113

RESUMO

Macromolecular films are crucial functional materials widely used in the fields of mechanics, electronics, optoelectronics, and biology, due to their superior properties of chemical stability, small density, high flexibility, and solution-processing ability. Their electronic and mechanical properties, however, are typically much lower than those of crystalline materials, as the macromolecular films have no long-range structural ordering. The state-of-the-art for producing highly ordered macromolecular films is still facing a great challenge due to the complex interactions between adjacent macromolecules. Here, the growth of textured macromolecular films on a designed graphene/high-index copper (Cu) surface is demonstrated. This successful growth is driven by a patterned potential that originates from the different amounts of charge transfer between the graphene and Cu surfaces with, alternately, terraces and step edges. The textured films exhibit a remarkable improvement in remnant ferroelectric polarization and fracture strength. It is also demonstrated that this growth mechanism is universal for different macromolecules. As meter-scale graphene/high-index Cu substrates have recently become available, the results open a new regime for the production and applications of highly ordered macromolecular films with obvious merits of high production and low cost.

20.
Aging (Albany NY) ; 13(12): 16513-16526, 2021 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-34157681

RESUMO

Colon adenocarcinoma (COAD) is a common cancer of the digestive system. It's high morbidity and mortality make it one of the leading causes of cancer deaths. In this study, we studied the microenvironment of colon cancer to find new diagnostic markers and immunotherapy targets for colon cancer. Tumor purity of colon cancer samples in TCGA database were obtained by ESTIMATE algorithm. Then, we analyzed the association of Immune, Stromal, and Estimate scores with tumor prognosis and clinicopathological features. By comparing the gene expression profiles between tumor and normal samples, the high and low immune score groups, 117 intersecting differentially expressed genes (DEGs) were obtained. The function, molecular pathway, and prognostic value of these 117 DEGs pointed toward the importance of deoxyribonuclease 1-like 3 (DNASE1L3). Validation results from multiple databases showed low expression of DNASE1L3 in colon cancer. A single GSEA and correlation analysis of immune cells indicated that DNASE1L3 was closely related to immunity. The low expression of DNASE1L3 in colon cancer samples was measured with qRT-PCR. The scratch and cell proliferation experiments suggested that DNASE1L3 may affect cell migration. Therefore, we concluded that DNASE1L3 might be a biomarker associated with prognosis and immune infiltration in colon cancer.


Assuntos
Biomarcadores Tumorais/metabolismo , Neoplasias do Colo/imunologia , Endodesoxirribonucleases/metabolismo , Linfócitos do Interstício Tumoral/imunologia , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Neoplasias do Colo/genética , Neoplasias do Colo/patologia , Bases de Dados Genéticas , Endodesoxirribonucleases/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Prognóstico , Mapas de Interação de Proteínas/genética , Reprodutibilidade dos Testes , Análise de Sobrevida , Transcriptoma
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