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1.
J Hematol Oncol ; 13(1): 9, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32005272

RESUMO

BACKGROUND: PARP inhibitors have been the most promising target drugs with widely proven benefits among ovarian cancer patients. Although platinum-response, HR-related genes, or HRD genomic scar detection are acceptably used in assessment of Olaparib response, there are still evident limitations in the present approaches. Therefore, we aim to investigate more accurate approaches to predict Olaparib sensitivity and effective synergistic treatment strategies. METHODS: We probed two databases (TCGA and Qilu Hospital) in order to quest novel miRNAs associated with platinum-sensitivity or HR-related genes. Cellular experiments in vitro or in vivo and PDX models were utilized to validate their role in tumor suppression and Olaparib sensitizing. Furthermore, HR gene mutation was analyzed through WES to explore the relation between HR gene mutation and Olaparib response. RESULTS: High miR-509-3 expression indicated better response to platinum and longer progression-free and overall survival in two independent ovarian cancer patient cohorts (high vs. low miR-509-3 expression; PFS: TCGA P < 0.05, Qilu P < 0.05; OS: TCGA P < 0.05, Qilu P < 0.01). MiR-509-3 could impair the proliferation, migration, and invasion ability but enhance the sensitivity to Olaparib of ovarian cancer cell in vitro and in vivo by directly targeting HMGA2 and RAD51. In two PDX cases (PDX1 and PDX9), miR-509-3 could significantly increase the sensitivity to Olaparib along with the decrease of RAD51 positive rate (mean tumor weight NC + Olaparib vs. miR-509 + Olaparib; PDX1 P < 0.05, PDX9 P < 0.05). Additionally, in PDX8, miR-509-3 treatment dramatically reversed the Olaparib insensitivity (P < 0.05) by downregulating RAD51 expression. RAD51 functional detection revealed that all Olaparib sensitive cases exhibited low RAD51 positive rate (lesser than 50%) in treated groups. Furthermore, among the four HR gene mutation patients, three harbored HR core gene mutation and were sensitive to Olaparib while the remaining one with non-HR core gene mutation did not respond well to Olaparib. CONCLUSIONS: MiR-509-3 can sensitize ovarian cancer cells to Olaparib by impeding HR, which makes it a potential target in PARPi synergistic treatment. HR core gene analysis and RAD51 functional detection are prospectively feasible in prediction of PARPi response.

2.
J Agric Food Chem ; 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32045247

RESUMO

In this work, we have developed an all-in-one aptasensor based on enzyme driven three-dimensional DNA walker for the antibiotic detection. To overcome the drawback of time-consuming, high density substrate strands were anchored on the walking interface that accelerating the signal amplification efficiency. Such all-in-one design integrated the functionality of target recognition, signal amplification as well as signal output into a single probe. Upon adding kanamycin, the activated walking strand moved along the track by the stepwise cleavage of nicking enzyme, which resulting the enhancement of the fluorescence intensity of solution. Under the optimized conditions, the detection process was accomplished in 40 min with a low detection limit of 1.23 pM. This aptasensor was also applied in spiked milk samples with satisfactory recoveries of 97.76 to 105.33%, demonstrating an excellent stability and accuracy. Therefore, this all-in-one aptasensor shows great potential for the application in food safety.

3.
J Digit Imaging ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040669

RESUMO

The grading of glioma has clinical significance in determining a treatment strategy and evaluating prognosis to investigate a novel set of radiomic features extracted from the fractional anisotropy (FA) and mean diffusivity (MD) maps of brain diffusion tensor imaging (DTI) sequences for computer-aided grading of gliomas. This retrospective study included 108 patients who had pathologically confirmed brain gliomas and DTI scanned during 2012-2018. This cohort included 43 low-grade gliomas (LGGs; all grade II) and 65 high-grade gliomas (HGGs; grade III or IV). We extracted a set of radiomic features, including traditional texture, morphological, and novel deep features derived from pre-trained convolutional neural network models, in the manually-delineated tumor regions. We employed support vector machine and these radiomic features for two classification tasks: LGGs vs HGGs, and grade III vs IV. The area under the receiver operating characteristic (ROC) curve (AUC), accuracy, sensitivity, and specificity was reported as the performance metrics using the leave-one-out cross-validation method. When combining FA+MD, AUC = 0.93, accuracy = 0.94, sensitivity = 0.98, and specificity = 0.86 in classifying LGGs from HGGs, while AUC = 0.99, accuracy = 0.98, sensitivity = 0.98, and specificity = 1.00 in classifying grade III from IV. The AUC and accuracy remain close when features were extracted from only the solid tumor or additionally including necrosis, cyst, and peritumoral edema. Still, the effects in terms of sensitivity and specificity are mixed. Deep radiomic features derived from pre-trained convolutional neural networks showed higher prediction ability than the traditional texture and shape features in both classification experiments. Radiomic features extracted on the FA and MD maps of brain DTI images are useful for noninvasively classification/grading of LGGs vs HGGs, and grade III vs IV.

4.
Sci Total Environ ; 713: 136634, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-32019021

RESUMO

Huge amounts of plastic waste are dumped into the ocean every year, forming large Garbage Patches. Countless microplastics, originating from fragmentation, weathering of larger objects or primary sources, pose a widespread ecological risk. In this study, the dispersion of suspended and floating microplastic particles in the East China Seas (ECSs) and adjacent seas was investigated via a coupled numerical model that included a Lagrangian particle tracking module. The role of tidal dynamics was considered in transporting the microplastic particles in the ECSs and adjacent seas. The results highlighted significant differences between the transport of suspended and floating microplastic particles. Although microplastic particles originating from different source areas followed different pathways, the Taiwan Strait, the Tokara Strait and the Tsushima Strait were identified as the major delivery channels. Of these, the Taiwan Strait played the most important role in the export of near-surface floating microplastic particles from the ECSs. The results showed that only a small fraction of the microplastic particles produced from the coastal waters of China (~18%) and Korea (~14%) entered the Pacific Ocean. However, nearly all of the microplastic particles originating from the west and south coasts of Kyushu Island entered the Pacific Ocean.

5.
Development ; 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-32001440

RESUMO

Sex determination and differentiation are complex processes controlled by many different factors; however, the relationships among these factors are poorly understood. Zebrafish gonadal differentiation exhibits high plasticity involving multiple factors and pathways, which provides an excellent model for investigating the interactions among them. Ovarian aromatase (cyp19a1a) and dmrt1 are key factors in directing vertebrate ovary and testis differentiation, respectively. Knockout of zebrafish cyp19a1a led to all-male offspring, whereas the loss of dmrt1 resulted in a female-biased sex ratio. In the present study, we established dmrt1 -/-;cyp19a1a -/- double mutant zebrafish and discovered that the introduction of the dmrt1 mutation into the cyp19a1a mutant could rescue the all-male phenotype of the latter. Interestingly, despite the lack of aromatase/estrogens, the follicles in the ovary of the rescued cyp19a1a mutant could develop normally up to the previtellogenic stage (PV). Further evidence suggested the ovarian aromatase directed ovarian differentiation by suppressing dmrt1 expression via nuclear estrogen receptors (nERs). Our results provide solid evidence for an interaction between cyp19a1a and dmrt1 in zebrafish gonadal differentiation and for the dispensability of estrogens in controlling early folliculogenesis.

6.
Clin Epigenetics ; 12(1): 19, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31992357

RESUMO

BACKGROUND: 5-Hydroxymethylcytosine (5hmC) is a novel epigenetic mark and may be involved in the mechanisms of tumorigenesis and malignant transformation. However, the role of 5hmC in ependymoma, the third most common brain tumor in children, remains unclear. The aim of this study sought to identify the characterization of 5hmC levels in pediatric posterior fossa ependymoma and to evaluate whether 5hmC levels could be a potential factor to predict clinical outcomes. RESULTS: Our results showed that 5hmC levels were globally decreased in posterior fossa ependymoma compared with normal cerebellum tissues (P < 0.001). Group A posterior fossa ependymomas had higher 5hmC levels than group B tumors (P = 0.007). Moreover, 5hmC levels positively correlated with Ki-67 index in posterior fossa ependymoma (r = 0.428, P = 0.003). Multivariate Cox hazards model revealed that patients with high 5hmC levels (> 0.102%) had worse PFS and OS than patients with lower 5hmC levels (< 0.102%) (PFS: HR = 3.014; 95% CI, 1.040-8.738; P = 0.042; OS: HR = 2.788; 95% CI, 0.974-7.982; P = 0.047). CONCLUSIONS: Our findings suggest that loss of 5hmC is an epigenetic hallmark for pediatric posterior fossa ependymoma. 5hmC levels may represent a potential biomarker to predict prognosis in children with posterior fossa ependymoma.

7.
Sci China Life Sci ; 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31942687

RESUMO

In China, the medical guidelines recommend performing noninvasive prenatal testing (NIPT) with caution for pregnant women aged 35 years or older. However, the Mother and Child Health Care Law suggests that all primiparous women whose age is older than 35 years undergo prenatal diagnosis. These two inconsistent suggestions/recommendations have made obstetricians confused about whether to offer NIPT to these older pregnant women. To face this issue and find out the solution we performed a retrospective study of 189,809 NIPT samples collected from 28 provincial-leveled administrative units in China. Of 1,564 women with high-risk pregnancies who underwent NIPT, 459 (29.3%) did not participate in follow-up. The compound sensitivity and specificity of NIPT for trisomies 21, 18 and 13 detection was 99.1% (95% CI, 98.0%-99.6%) and 99.9% (95% CI, 98.8%-99.9%), respectively. In secundiparous women, NIPT showed high sensitivity and specificity similar to that in primiparous women. The observed risk for trisomies 21 and 18 significantly increased when the maternal age was 39 and older. After the publication of the current NIPT policy, the follow-up rate at our center was 91.9%; however, a large number of women are not in maternal and infant care networks nationwide, and that makes the follow-up rate outside our center relatively low. Our study shows that to balance the prevention of major aneuploidies and the limited resources for prenatal diagnosis, the cut-off age of 35 for invasive prenatal diagnosis might be unnecessary. Although the NIPT guidelines are well written, how to practice it effectively, especially in less industrialized areas, is worth discussing.

8.
Physiol Meas ; 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31972550

RESUMO

OBJECTIVE: This paper aims to present that physiological signals are processed based on wavelet decomposition to calculate multiple physiological parameters in real-time on an embedded platform. APPROACH: ECG and PPG were decomposed to the appropriate scale based on the quadratic spline wavelet base in order to get the high and narrow pulse peaks at the location of the mutation points. Based on the decomposed waveforms, feature points are positioned to calculate physiological parameters in real-time, including heart rate, pulse rate, blood oxygen, and blood pressure. The proposed algorithm has been implemented on TI's CC2640R2F. MAIN RESULTS: The misdetection rate of feature points location based on the square wavelet decomposition waveform is only 0.57% in the acquired ECG and 0.23% in the acquired PPG. Heart rate and pulse rate are both highly correlated with references whose correlation coefficients are both 0.99. The pulse rate and heart rate were 3.85% (51/1326)and 2.94%(39/1326) outside the 95% consistency limit, respectively. Systolic and diastolic blood pressures are significantly associated with standard equipment whose correlation coefficients are 0.87 and 0.83. Systolic and diastolic blood pressures were 5.88% (21/357) and 5.32% (19/357) outside the 95% consistency limit, respectively. SIGNIFICANCE: the real-time calculation of multiple physiological parameters based on wavelet decomposition on an embedded platform has outstanding accuracy.

9.
Mol Med Rep ; 21(2): 720-730, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31974609

RESUMO

In the present study, the mechanism by which carboxyl terminal activating region 3 (CTAR3) of latent membrane protein 1 (LMP1), encoded by the Epstein­Barr virus, regulated cell proliferation and protein expression was investigated in the nasopharyngeal epithelial cell line NP69. The deletion mutant LMP1 (LMP1Δ232­351; amino acid residues including 232­351 codons in CTAR3 deleted) was generated by polymerase chain reaction. An NP69­LMP1Δ232­351 cell line was established by retroviral infection. Finally, cell proliferation and protein expression of NP69 cells expressing LMP1Δ232­351 were examined using a cell growth curve and western blot analysis. The results demonstrated: i) The proliferation of NP69­LMP1Δ232­351 cells was significantly decreased compared with cells expressing wild type LMP1 (LMP1WT; n=3; P<0.05); ii) 17 proteins exhibited differential protein expression (>2­fold change) in NP69­LMP1Δ232­351 cells compared with NP69­LMP1WT cells; and iii) LMP1WT was involved in activating the Janus kinase 3 (JAK3) promoter and regulating the expression of JAK3 protein, while LMP1Δ232­351 was almost defective in ability to activate the JAK promoter. These results suggested that LMP1­CTAR3 may be an important functional domain for regulating cell proliferation and protein expression in nasopharyngeal epithelial cells.

10.
J Org Chem ; 85(2): 537-547, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31808693

RESUMO

Direct conversion of the readily available alkyl bromides and alcohols to value-added epoxides using dimethyl sulfoxide (DMSO) under mild reaction conditions has been developed. Benzyl and allyl bromides, and activated and unactivated alcohols all proceeded smoothly to give epoxides in high to excellent yield. Dimethyl sulfide, generated by DMSO oxidations, was in situ elaborated to form the substituted dimethyl sulfonium ylide species that participates in the Corey-Chaykovsky epoxidation in a domino and one-pot fashion, respectively.

11.
J Cell Mol Med ; 24(1): 356-361, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31729134

RESUMO

Increasing studies have revealed that circular RNAs (circRNAs) play important roles in cancer progression. However, the potential involvement of circRNAs in breast cancer metastasis to lung is not clear so far. In this study, we conducted circular RNA microarrays of primary breast cancer tissues and lung metastatic tissues. The results revealed that circFBXL5 (hsa_circ_0125597) up-regulated the most in lung metastatic tissues. Survival analysis revealed that high levels of circFBXL5 correlated with worse outcome of breast cancer. Further experiments showed that knockdown of circFBXL5 inhibited breast cancer cell proliferation and migration to lung. Mechanism study showed that circFBXL5 acted as a sponge for miR-660 and compete binding to miR-660 with SRSF6, leading to increased expression of SRSF6. Collectively, our study highlighted the regulatory function of the circFBXL5/miR-660/SRSF6 pathway in breast cancer progression, which could be potential therapeutic targets for breast cancer.

12.
J Cell Physiol ; 235(4): 3776-3789, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31602654

RESUMO

Fatty acids (FAs) play a crucial role in the development of clear cell renal cell carcinoma (ccRCC), FAs function requires the participation of fatty-acid-binding protein (FABP). Current studies have shown that different members of the FABP's family play different roles in the tumorigenesis of ccRCC. Therefore, the systematic analysis of FABPs will be of great significance. However, the diverse expression patterns and prognostic values of nine FABPs have yet to be elucidated. In this study, through multiple analysis and verification of multiple databases, such as ONCOMINE, The Human Protein Atlas, UALCAN, Gene Expression Profiling Interactive Analysis, and cBioPortal, we found that the expression of FABP1 was significantly downregulated and the expression of FABP5/6/7 was significantly upregulated in ccRCC compared with renal tissues, and the patients with high messenger RNA (mRNA) levels of the FABP5/6/7 or low mRNA levels of FABP1 were predicted to have a lower overall survival or disease-free survival. Further analysis by the protein-protein interaction (PPI), Gene Ontology pathway, and Kyoto Encyclopedia of Genes and Genomes pathway showed that FABPs were mainly involved in the peroxisome proliferator-activated receptor (PPAR) pathway. In coexpression analysis, we found that FABP1/5/6/7 was coexpressed with transforming growth factor-ß1 (TGF-ß1), PPARA, and LPL. This study implied that FABP1/5/6/7 could act as an important tumor biomarker of ccRCC; the role of FABPs may be related to PPAR or TGF-ß pathway.

13.
Gastrointest Endosc ; 91(1): 41-51, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31445040

RESUMO

BACKGROUND AND AIMS: We developed a system for computer-assisted diagnosis (CAD) for real-time automated diagnosis of precancerous lesions and early esophageal squamous cell carcinomas (ESCCs) to assist the diagnosis of esophageal cancer. METHODS: A total of 6473 narrow-band imaging (NBI) images, including precancerous lesions, early ESCCs, and noncancerous lesions, were used to train the CAD system. We validated the CAD system using both endoscopic images and video datasets. The receiver operating characteristic curve of the CAD system was generated based on image datasets. An artificial intelligence probability heat map was generated for each input of endoscopic images. The yellow color indicated high possibility of cancerous lesion, and the blue color indicated noncancerous lesions on the probability heat map. When the CAD system detected any precancerous lesion or early ESCCs, the lesion of interest was masked with color. RESULTS: The image datasets contained 1480 malignant NBI images from 59 consecutive cancerous cases (sensitivity, 98.04%) and 5191 noncancerous NBI images from 2004 cases (specificity, 95.03%). The area under curve was 0.989. The video datasets of precancerous lesions or early ESCCs included 27 nonmagnifying videos (per-frame sensitivity 60.8%, per-lesion sensitivity, 100%) and 20 magnifying videos (per-frame sensitivity 96.1%, per-lesion sensitivity, 100%). Unaltered full-range normal esophagus videos included 33 videos (per-frame specificity 99.9%, per-case specificity, 90.9%). CONCLUSIONS: A deep learning model demonstrated high sensitivity and specificity for both endoscopic images and video datasets. The real-time CAD system has a promising potential in the near future to assist endoscopists in diagnosing precancerous lesions and ESCCs.

14.
Biomed Pharmacother ; 121: 109562, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31707341

RESUMO

BACKGROUND: Lung cancer has been the most common cancer worldwide. Microsomal glutathione S-transferase 1 (MGST1) has been reported to play vital roles in oxidative stress, tumor occurrence and drug resistance. However, the biological function and molecular mechanism of MGST1 in lung adenocarcinoma (LUAD) has not yet been elucidated. METHODS: The expression of MGST1 in LUAD tissues and cell lines was evaluated by immunohistochemistry and western blotting, respectively. MGST1 was knocked down by shRNA lentivirus. Cell proliferation was evaluated by MTS, colony formation and EdU assays. Apoptosis was detected by flow cytometry. The potential molecules involved in cell proliferation and apoptosis were examined by western blotting. Finally, the effect of MGST1 on tumor growth in vivo was evaluated in a nude mouse xenograft model. RESULTS: TCGA database analysis and immunohistochemistry demonstrated that MGST1 was highly expressed in LUAD tissues. MGST1 expression in LUAD was correlated with AJCC stage and poor overall survival of patients. MGST1 knockdown significantly inhibited LUAD cell proliferation and induced apoptosis. Mechanistic analyses revealed that MGST1 knockdown might inhibit cell proliferation by inactivating the AKT/GSK-3ß pathway signaling and promote cell apoptosis by regulating the mitochondrial apoptosis pathway related proteins. Moreover, knockdown of MGST1 suppressed tumor growth in vivo. CONCLUSIONS: MGST1 plays an important role in LUAD tumorigenesis and might serve as a potential prognostic factor and therapeutic target in LUAD.

15.
J Nanosci Nanotechnol ; 20(4): 2578-2583, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492279

RESUMO

Quaternary colloidal CuIn0.7Ga0.3S2 nanocrystals were synthesized by hot injection method using oleylamine as single solvent. The structure, morphology and optical absorption properties of assynthesized CuIn0.7Ga0.3S2 nanocrystals were characterized in detail using X-ray powder diffraction, transmission electron microscopy and UV-vis spectrometry. The as-fabricated CIGSSe thin film was obtained by spin-casting as-synthesized colloidal CuIn0.7Ga0.3S2 nanocrystals after selenization. The classical pn heterojunction CIGSSe/CdS thin film solar cells displayed the 6.18% power conversion efficiency under AM1.5G illumination.

17.
J Hazard Mater ; 386: 121886, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31887561

RESUMO

Bisphenol A (BPA) can induce endocrine disorders in humans and animals. In this study, we used several zebrafish mutants deficient in estrogen production and signalling, which could be valuable for evaluating estrogenic activities and mechanisms of EDCs. With low endogenous estrogens, the all-male aromatase mutant (cyp19a1a-/-) is expected to be more responsive to estrogenic exposure, and mutants of nuclear estrogen receptors (nERs; esr1-/-, esr2a-/- and esr2b-/-) alone or in combination would allow us to evaluate the action mechanisms of estrogenic EDCs. Exposure to BPA could rescue the all-male phenotype of the cyp19a1a-/- mutant, delayed gonadal development in both sexes, resulting in infertility or subfertility, and caused follicle atresia in females and impairment of spermatogenesis in males. To understand the mechanisms of these effects, we tested BPA in cyp19a1a and nER mutants of different combinations. The feminizing effect of BPA on sexual differentiation was dependent on nERs, in particular esr2a. As for males, nERs were also involved in BPA-induced impairment of spermatogenesis. Taken together, with genome editing technology our study provides the most comprehensive genetic evidence for estrogenic activities of BPA in zebrafish and its action mechanisms. This study also establishes a powerful platform for studying other EDCs with estrogenic activity.

18.
Pharmgenomics Pers Med ; 12: 319-327, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31802929

RESUMO

Background: Emery-Dreifuss muscular dystrophy, caused by mutations in genes such as emerin (EMD) or lamin A/C (LMNA), is a disorder affecting the joints, muscles, and heart, with a wide spectrum of patient phenotypes including muscle wasting and cardiac conduction defects. Methods and results: Here we report a multi-generation family from the Hunan Province of China. Affected family members displayed an uncommon clinical presentation of serious cardiac conduction abnormalities at an early age and a high incidence of sudden cardiac death along with mild skeletal muscular atrophy and joint contracture. Clinical analysis of affected members provided evidence of X-linked recessive inheritance. Consequently, using Sanger sequencing of X chromosome exomes, we identified a novel duplication mutation (c.405dup/p.Asp136X) in the EMD gene as the cause for the disease in this family. This variant is a novel mutation that has not been previously reported in Pubmed, Clinvar or other cases reported in the Human Gene Mutation Database. Conclusion: Our finding expands the mutation spectrum of Emery-Dreifuss muscular dystrophy and provides a rationale for EMD mutation testing in cases of X-linked inherited cardiac conduction disease and sudden cardiac death, even in those lacking pathognomonic neuromuscular features.

19.
Ying Yong Sheng Tai Xue Bao ; 30(11): 3903-3910, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31833704

RESUMO

Nematodes, occupying multiple trophic levels in the food web, play important roles in energy flow and nutrient cycling. Most of Chinese natural grasslands have been degraded due to long-term unreasonable utilization, such as over-grazing. External nutrient input is an important way to restore the ecological function of degraded grasslands. The main and intertative effects of nitrogen and phosphorus inputs on soil nematode abundance, trophic group composition and community structure were studied in the grasslands in Xilingol League of Inner Mongolia. Totally, 38 genera of nematodes were recorded. Tylencholaimus, Aphelenchoides, Thonus, and Scutylenchus were dominant genera in this degraded grassland. Nitrogen input decreased total abundances of soil nematodes, and that of omnivores-carnivorous nematodes and plant-feeding nematodes. Phosphorus input increased total abundances of soil nematodes, and that of fungal-feeding nematodes, omnivores-carnivorous nematodes, and plant-feeding nematodes. Nitrogen input inhibited the positive effects of phosphorus input on the abundances of total nematodes, omnivores-carnivorous nematodes and plant-feeding nematodes. Nutrient inputs had no effect on nematode diversity, which would be resulted from the stable plant community. Nitrogen input significantly increased nematode maturity index, decreased plant parasitic nematode maturity index (PPI), and greatly alleviated the negative effects of phosphorus input on PPI and Wasilewska index, indicating that nitrogen input could improve soil health condition and the stability of nematodes community. Our results would help improve our understanding of the effects of nutrient inputs on degraded grassland ecosystem from a soil biotic perspective.


Assuntos
Nematoides , Solo , Animais , China , Ecossistema , Pradaria , Nitrogênio , Fósforo
20.
Artigo em Inglês | MEDLINE | ID: mdl-31828306

RESUMO

KLF7, one of candidate genes in neurotherapy and metabolic syndrome, has been studied in adipogenesis of mammalian species and birds. However, the effect of the third C2H2 zinc finger of KLF7 for its transcriptional regulation in adipogenesis has not been well understood. Here, the wild-type chicken KLF7 (KLF7) overexpression plasmid, pCMV-myc-KLF7, and two plasmids of chicken KLF7 mutants, i.e. pCMV-myc-KLF7m1 with half of the third zinc finger (KLF7m1) and pCMV-myc-KLF7m2 without the third zinc finger (KLF7m2), were constructed. Luciferase reporter assay in DF1 cells showed that the effect of chicken KLF7 overexpression on the promoter activity of LPL was greater than those of KLF7m1 and KLF7m2 (P < 0.05). There was no significant difference among the overexpression of KLF7, KLF7m1 and KLF7m2 on the promoter activities of FASN, C/EBPα and FABP4 (P > 0.05). Additionally, the effects of KLF7, KLF7m1 and KLF7m2 overexpression on the promoter activity of PPARγ were different. KLF7 overexpression had no significant effect on the PPARγ promoter activity (P > 0.05), KLF7m1 overexpression suppressed PPARγ promoter activity (P < 0.05), while KLF7m2 overexpression facilitated the promoter activity of PPARγ (P < 0.05), consistent with the results of western blot analysis. Our results suggested that the third zinc finger of chicken KLF7 may play a role in its transcriptional regulation of LPL and PPARγ but has no effect on its regulation of C/EBPα, FASN and FABP4. The third zinc finger of KLF7 might be a target for the treatment of metabolic disorder in chicken.

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