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1.
Zhongguo Zhong Yao Za Zhi ; 43(1): 100-108, 2018 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-29552818

RESUMO

Application of a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, macroporous adsorbent resin, and reversed-phase HPLC, led to the isolation of 173 compounds including irdidoids, monoterpenes, sesquiterpenes, triterpenes, lignans, flavonoids, and simple aromatic derivatives from the ethyl acetate-soluble fraction of the whole plants of Valeriana jatamansi(Valerianaceae), and their structures were elucidated by spectroscopic methods including 1D, 2D NMR UV, IR, and MS techniques. Among them, 77 compounds were new. In previous reports, we have described the isolation, structure elucidation, and bioactivities of 68 new and 25 known compounds. As a consequence, we herein reported the isolation and structure elucidation of the remaining 9 new and 71 known compounds, the structure revision of valeriotriate A(8a), as well as cytotoxicity of some compounds.


Assuntos
Extratos Vegetais/química , Valeriana/química , Acetatos , Cromatografia Líquida de Alta Pressão , Flavonoides/análise , Iridoides/análise , Lignanas/análise , Estrutura Molecular , Monoterpenos/análise , Compostos Fitoquímicos/análise , Sesquiterpenos/análise , Triterpenos/análise
3.
Planta Med ; 81(1): 56-61, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25469856

RESUMO

Ten new valepotriates, jatamanvaltrates P-Y (1-10) and a known one, nardostachin (11), have been isolated from the whole plants of Valeriana jatamansi. The structures of the new compounds were determined by detailed spectroscopic data analysis. The isolated compounds were evaluated for cytotoxic activity against PC-3M cells, and a structure-activity relationship was examined for all the valepotriates isolated from V. jatamansi. The results highlighted the structure-activity relationship importance of the C-3-C-4 double bond, the oxirane ring, and the 10-chlorine in the valepotriates.


Assuntos
Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Neoplasias da Próstata/tratamento farmacológico , Valeriana/química , Linhagem Celular Tumoral/efeitos dos fármacos , Humanos , Iridoides/química , Iridoides/farmacologia , Espectroscopia de Ressonância Magnética , Masculino , Estrutura Molecular , Neoplasias da Próstata/patologia , Relação Estrutura-Atividade
4.
Zhonghua Er Ke Za Zhi ; 51(11): 846-8, 2013 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-24484560

RESUMO

OBJECTIVE: To discuss the effect of electric coagulation through bronchoscopy in diagnosis and treatment of congenital vallecular cyst in children. METHOD: Ten cases of congenital vallecular cyst in the study with age ranged from 21 days to 4 years and 10 months were treated with electric coagulation through bronchoscopy. The therapeutic effect was evaluated by endoscopic and clinical manifestation. And all the patients were followed-up for 6-12 months. RESULT: All the patients obtained 3-5 times electric coagulation. After the operation, the cyst decreased in size, epiglottis softening was subsided, uplift uncompression, dyspnea and laryngeal stridor were improved obviously. After follow-up periods of 6-12 months, no capsule wall were left, and the activity of the epiglottis resumed.No severe complication was found in any patient. CONCLUSION: Electric coagulation through bronchoscopy is a simple, effective and safe method to treat congenital vallecular cyst in children.


Assuntos
Broncoscopia/métodos , Cistos/cirurgia , Eletrocoagulação , Epiglote/patologia , Doenças da Laringe/cirurgia , Pré-Escolar , Cistos/congênito , Cistos/diagnóstico , Dispneia/etiologia , Dispneia/fisiopatologia , Epiglote/cirurgia , Feminino , Seguimentos , Humanos , Lactente , Recém-Nascido , Doenças da Laringe/congênito , Doenças da Laringe/diagnóstico , Masculino , Sons Respiratórios/etiologia , Sons Respiratórios/fisiopatologia , Estudos Retrospectivos , Resultado do Tratamento
5.
Phytochemistry ; 85: 185-93, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23036722

RESUMO

HPLC-PDA-MS and TLC analysis were used to look for minor cytotoxic chlorinated valepotriates from whole plants of Valeriana jatamansi (syn. Valeriana wallichii DC.). This resulted in isolation of 15 chlorinated valepotriates, designated as chlorovaltrates A-O, together with six known analogues, (1S,3R,5R,7S,8S,9S)-3,8-epoxy-1,5-dihydroxyvalechlorine, volvaltrate B, chlorovaltrate, rupesin B, (1S,3R,5R,7S,8S,9S)-3,8-epoxy-1-O-ethyl-5-hydroxyvalechlorine, and (1R,3R,5R,7S,8S,9S)-3,8-epoxy-1-O-ethyl-5-hydroxyvalechlorine. Their structures were elucidated by spectroscopic methods including homo- and heteronuclear two-dimensional NMR experiments. Chlorovaltrates K-N, chlorovaltrate and rupesin B showed moderate cytotoxicity against lung adenocarcinoma (A 549), metastatic prostate cancer (PC-3M), colon cancer (HCT-8) and hepatoma (Bel 7402) cell lines with IC50 values of 0.89-9.76 µM.


Assuntos
Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Iridoides/química , Iridoides/farmacologia , Valeriana/química , Linhagem Celular Tumoral , Humanos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular
6.
Zhonghua Er Ke Za Zhi ; 50(1): 45-9, 2012 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-22456075

RESUMO

OBJECTIVE: To analyze the effect and safety of bronchoscopic cryosurgery in children with lower airway stenosis caused by granulation and fiber hyperplasia. METHOD: Twenty-two patients had undergone bronchoscopic examination and cryosurgery, 18 patients with atelectasis caused by Mycoplasma pneumoniae pneumonia, 2 patients with endobronchial tuberculosis (EBTB), 1 patient with atelectasis caused by granulation tissue after bronchial foreign body, and 1 case who had severe dyspnea, with tracheal stenosis after long-term endotracheal intubation during surgery for heart disease. All the patients under went bronchoscopic cryosurgery for several times, 1 case with severe tracheal stenosis was operated by electric coagulation before cryosurgery. Before and after the treatment, all the patients were evaluated based on clinical symptoms, tracheal lumen by bronchoscopy, chest CT for atelectasis and pulmonary function for tracheal stenosis. RESULT: The patients were treated with cryosurgery for 1-4 times. Sixteen cases were markedly improved, clinical symptoms disappeared completely, no granulation and fiber hyperplasia were found during bronchoscopy, and all the atelectasis were cured. After the treatment, the bronchial lumen diameter of the patient with tracheal stenosis was increased from 2 mm to about 5 mm, the tidal volume increased from 3.0 ml/kg to 8.8 ml/kg. Five cases were effectively improved, the clinical symptoms also disappeared, during bronchoscopy and bronchial alveolar lavage (BAL), no granulation and fiber hyperplasia were found, but some distal bronchial tubes were atresia or narrowed, the lumen was patent, the atelectasis recovered partially. In 1 case the treatment was ineffective, as the clinical symptoms and granulation disappeared, but because almost all distal bronchi were atresia or narrow, the lumen was not patent during BAL, there was no change on chest CT. The total effective rate was 21/22 (95.5%). No severe complications occurred during and after the procedures. All the patient were followed up for 1 - 12 months, no case had recurrence. CONCLUSION: Treatment with bronchoscopic cryosurgery is a safe and effective methods to tracheal stenosis caused by granulation tissue and fiber hyperplasia in children.


Assuntos
Broncopatias/cirurgia , Criocirurgia , Pneumopatias/cirurgia , Broncopatias/etiologia , Broncoscopia , Criança , Pré-Escolar , Cicatriz/complicações , Constrição Patológica , Feminino , Granuloma/complicações , Humanos , Pneumopatias/etiologia , Masculino , Estenose Traqueal/etiologia , Estenose Traqueal/cirurgia
7.
Chem Commun (Camb) ; 47(37): 10413-5, 2011 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-21833427

RESUMO

Cyclopenicillone (1), possessing a unique 2,5-dimethylcyclopent-2-enone carbon skeleton, has been isolated from the cultures broth of the fungus Penicillium decumbens. The structure and absolute configuration of 1 were elucidated using a combination of NMR spectroscopy, CD data and computational approaches. Cyclopenicillone (1) demonstrated a dose-dependent (10-100 µM) inhibition against LPS-induced NO production in RAW264.7 macrophages.


Assuntos
Técnicas de Cultura , Ciclopentanos/química , Penicillium/crescimento & desenvolvimento , Linhagem Celular , Ciclopentanos/isolamento & purificação , Ciclopentanos/metabolismo , Ciclopentanos/farmacologia , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Modelos Moleculares , Conformação Molecular , Óxido Nítrico/biossíntese , Penicillium/metabolismo
8.
Zhonghua Er Ke Za Zhi ; 49(12): 947-51, 2011 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-22336365

RESUMO

OBJECTIVE: To evaluate the effect of bronchoalveolar lavage (BAL) through bronchoscopy in diagnosis and treatment of accidental inhalation of liquid and powdery foreign bodies in children. METHOD: Twenty-one cases of accidental inhalation were classified into the following 4 groups according to the materials inhaled: 3 cases inhaled the lipidic material; 2 cases inhaled chemical material; 15 cases inhaled hydrosoluble materials; 1 case inhaled powdery material. Eighteen cases were hospitalized within 3 days after the accidents, which included 16 cases treated within 1 day in hospital. And 3 cases were hospitalized 3 days after the accident. After hospitalization, all patients were immediately treated with direct aspiration and lavage clearance method through bronchoscopy. The inhaled foreign materials were cleared as soon as possible, which reduced the absorption, stimulation and infection of respiratory mucosa. The therapeutic effect was evaluated by endoscopic findings, clinical manifestation, laboratory examination and chest imaging. At the same time, decision was made on the second BAL and the prognosis was estimated. RESULT: Twenty-one cases had dyspnea of certain degree. The bronchial mucous membrane of all the 21 cases showed hyperemia, edema and exudate attached. Lavage fluid looked like broth and contained much of the inhaled material. After two to five times treatment, 18 cases were cured (86%), but 3 cases died (14%). One of these 3 cases fell into an oil well, 1 into mosquito repellent solution, another into manure pit. The patients who could not survive had severe airway obstruction, which was difficult to clean and made the problem deteriorated progressively. One of the patients was sent to a hospital 3 days after the accident and another case developed fungal infection, pneumothorax and finally respiratory failure. CONCLUSION: BAL through bronchoscopy is necessary for children who accidentally inhaled liquid and powdery materials. This treatment is safe and effective in diagnosis and treatment of such problems in children. The effect of treatment is influenced by types of inhaled material, the time of lavage clearance through bronchoscopy, and certain complications, such as infection etc.


Assuntos
Lavagem Broncoalveolar , Broncoscopia , Corpos Estranhos/terapia , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Exposição por Inalação , Masculino , Pós
9.
J Nat Prod ; 73(10): 1723-6, 2010 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-20853876

RESUMO

The structures of 1,5-dihydroxy-3,8-epoxyvalechlorine (1a) and volvaltrate B (6a), two new chlorinated iridoids isolated from Valeriana jatamansi and V. officinalis, respectively, were originally assigned on the basis of spectroscopic methods. Reinvestigation using X-ray analysis and chemical transformation revealed that the original assignment of H-7 in 1a and OH-8 in 6a should be inverted and that the structures should be revised to 1 and 6, respectively. Correspondingly, the structure of valeriotetrate C (7a) should be revised to 7. Volvaltrate B (6) showed cytotoxic activity against the lung adenocarcinoma (A549), metastatic prostate cancer (PC-3M), colon cancer (HCT-8), and hepatoma (Bel7402) cell lines, with IC50 values of 8.5, 2.0, 3.2, and 6.1 µM, respectively.


Assuntos
Antineoplásicos Fitogênicos/química , Medicamentos de Ervas Chinesas/química , Iridoides/química , Valeriana/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Neoplasias do Colo , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Iridoides/isolamento & purificação , Iridoides/farmacologia , Masculino , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Raízes de Plantas/química , Neoplasias da Próstata/tratamento farmacológico
10.
Zhonghua Er Ke Za Zhi ; 48(4): 301-4, 2010 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-20654022

RESUMO

OBJECTIVE: To assess the efficacy and safety of balloon dilatation through flexible bronchoscopy in the management of inflammatory stenosis of grade 4-5 bronchus. METHOD: Thirty patients with inflammatory bronchial stenosis caused by mycoplasmal pneumonia complicated with pulmonary atelectasis were treated with balloon dilatation through fiberoptic bronchoscopy. Before the procedure and after the last operation, therapeutic effect on pulmonary atelectasis were evaluated with CT and all of the patients were followed-up for 1 - 6 months. RESULT: One to three operations were required to achieve satisfactory dilatation. After balloon dilatation, the average airway diameter increased obviously and the farther airways were opened after the therapy with irrigation. In 25 of 30 cases satisfactory immediate effects were obtained, a narrow airway diameter above expansion significantly increased as compared with preoperative diameter. In 5 children treated with balloon dilatation, the stenosis could not be improved significantly. In 3 patients with hyperplasia of granulation tissue, cryotherapy had to be applied. The operations were ineffective in the other two patients whose course of disease exceeded 3 months. After follow-up periods of 1 - 6 months, chest CT manifestation of expanded sites was improved in 28 patients and atelectasis disappeared. No severe complication was found in any patients. CONCLUSION: Bronchoplasty by balloon dilatation through flexible fiberoptic bronchoscopy is a simple, effective and safe method to treat childhood tracheobronchial stenosis after pulmonary infections.


Assuntos
Cateterismo/métodos , Pneumonia por Mycoplasma/terapia , Atelectasia Pulmonar/terapia , Broncopatias/terapia , Broncoscopia , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Atelectasia Pulmonar/microbiologia , Estenose Traqueal
11.
Luminescence ; 20(4-5): 370-6, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16134211

RESUMO

A comparative study of the photoluminescence properties of three kinds of tetra-ring aromatic hydrocarbon (1-sodium pyrenesulphonate, benz[alpha]anthracene and chrysene) solution in the absence of any protecting medium is described. It was found that a room temperature phosphorescence signal with different intensities can be induced for these solutions, using only TlNO3 or KI as a heavy atom perturber (HAP) and Na2SO3 as a deoxygenator. An appropriate amount of organic solvent added to the systems of pyrene, benz[alpha]anthracene and chrysene is necessary for increasing the solubility and phosphorescence intensity, and the preferable solvent is acetonitrile. For the pyrene, pyrenesulphonate and chrysene systems, a delayed excimer fluorescence accompanied with the room temperature phosphorescence (RTP) emission can be observed, but that for benz[alpha]anthracene cannot. The ratio of delayed excimer fluorescence and phosphorescence signals for pyrene, pyrenesulphonate and chrysene systems can be controlled by adjusting the concentration of luminophor, kinds and amount of both organic solvents and HAP. Under the optimal conditions, the RTP signals are proportional to the concentration of the four aromatic hydrocarbons, which means that the RTP properties of the four tetra-ring aromatic hydrocarbons can be used for quantitative analysis.


Assuntos
Hidrocarbonetos Aromáticos/química , Temperatura , Ciclização , Dioxanos , Medições Luminescentes , Estrutura Molecular , Fotoquímica , Padrões de Referência , Sensibilidade e Especificidade , Solventes/química , Solventes/farmacologia , Espectrometria de Fluorescência
12.
Spectrochim Acta A Mol Biomol Spectrosc ; 58(10): 2185-91, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12212743

RESUMO

A direct and simple non-protected room temperature phosphorimetry (NP-RTP) for determine propranolol, which using I- as a heavy atom perturber and sodium sulfite as a deoxygenator, has been developed. The phosphorescence peak wavelength maxima lambda(ex)/lambda(em) = 288/494, 522 nm. The analytical curve of propranolol gives a linear dynamic range of 8.0 x 10(-8)-2.0 x 10(-5) mol l(-1) and a detection limit of 3 x 10(-8) mol l(-1). The influence of I- concentration on RTP lifetime of propranolol was studied and the luminescence kinetic parameters were calculated. It is found that the relation between I- concentration (x) and RTP lifetime (tau) can be expressed as tau = 1.25e(-0.477x) and the rate constants of phosphorescence emission k(p) was 0.800 per ms. The method was applied directly to determination of propranolol in urine and drug tablets with a satisfactory result. The recoveries were 96.6-97.4% and the relative standard deviation was 2% for the 1.00 x 10(-6)-4.00 x 10(-6) mol l(-1) propranolol in spiked urine sample.


Assuntos
Propranolol/química , Humanos , Indicadores e Reagentes , Luminescência , Propranolol/análise , Propranolol/urina , Espectrofotometria/métodos , Sulfitos , Temperatura , Termodinâmica
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