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1.
Appl Opt ; 60(23): 6742-6747, 2021 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-34613151

RESUMO

Circular dichroism (CD) demonstrates broad application prospects in enantioselective catalysis, chiral separation, and ultrasensitive detection. Increasing the CD intensity of easily fabricated plasmonic nanostructures will promote the application of these artificial nanostructures. A chiral plasmonic system that consists of two unaligned nanorods and a metal film is proposed in this study to achieve a large CD effect. Indirect coupling of a nanorod-film-nanorod in the proposed chiral plasmonic system generates a larger CD intensity compared to the direct coupling of a nanorod-nanorod. In addition, the effects of structural parameters on the CD effect of the proposed system are numerically investigated. Results showed that the indirect coupling is strongly dependent on the separation between the nanorod and the metal film. The results of this study can provide an effective strategy to enhance the CD effect of plasmonic chiral systems.

2.
Mol Biol Evol ; 2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34390581

RESUMO

Genetic variation and phenotypic plasticity are both important to adaptive evolution. However, how they act together on particular traits remains poorly understood. Here, we integrated phenotypic, genomic, and transcriptomic data from two allopatric but closely related congeneric oyster species, Crassostrea angulata from southern/warm environments and Crassostrea gigas from northern/cold environments, to investigate the roles of genetic divergence and plasticity in thermal adaptation. Reciprocal transplantation experiments showed that both species had higher fitness in their native habitats than in non-native environments, indicating strong adaptive divergence. The southern species evolved higher transcriptional plasticity, and the plasticity was adaptive, suggesting that increased plasticity is important for thermal adaptation to warm climates. Genome-wide comparisons between the two species revealed that genes under selection tended to respond to environmental changes and showed higher sequence divergence in noncoding regions. All genes under selection and related to energy metabolism exhibited habitat-specific expression with genes involved in ATP production and lipid catabolism highly expressed in warm/southern habitats, and genes involved in ATP consumption and lipid synthesis were highly expressed in cold/northern habitats. The gene for acyl-CoA desaturase, a key enzyme for lipid synthesis, showed strong selective sweep in the upstream noncoding region and lower transcription in the southern species. These results were further supported by the lower free fatty acid (FFA) but higher ATP content in southern species and habitat, pointing to significance of ATP/FFA trade-off. Our findings provide evidence that noncoding variation and transcriptional plasticity play important roles in shaping energy metabolism for thermal adaptation in oysters.

3.
Theranostics ; 11(16): 8112-8128, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34335983

RESUMO

The coiled-coil domain containing protein members have been well documented for their roles in many diseases including cancers. However, the function of the coiled-coil domain containing 65 (CCDC65) remains unknown in tumorigenesis including gastric cancer. Methods: CCDC65 expression and its correlation with clinical features and prognosis of gastric cancer were analyzed in tissue. The biological role and molecular basis of CCDC65 were performed via in vitro and in vivo assays and a various of experimental methods including co-immunoprecipitation (Co-IP), GST-pull down and ubiquitination analysis et al. Finally, whether metformin affects the pathogenesis of gastric cancer by regulating CCDC65 and its-mediated signaling was investigated. Results: Here, we found that downregulated CCDC65 level was showed as an unfavourable factor in gastric cancer patients. Subsequently, CCDC65 or its domain (a.a. 130-484) was identified as a significant suppressor in GC growth and metastasis in vitro and in vivo. Molecular basis showed that CCDC65 bound to ENO1, an oncogenic factor has been widely reported to promote the tumor pathogenesis, by its domain (a.a. 130-484) and further promoted ubiquitylation and degradation of ENO1 by recruiting E3 ubiquitin ligase FBXW7. The downregulated ENO1 decreased the binding with AKT1 and further inactivated AKT1, which led to the loss of cell proliferation and EMT signal. Finally, we observed that metformin, a new anti-cancer drug, can significantly induce CCDC65 to suppress ENO1-AKT1 complex-mediated cell proliferation and EMT signals and finally suppresses the malignant phenotypes of gastric cancer cells. Conclusion: These results firstly highlight a critical role of CCDC65 in suppressing ENO1-AKT1 pathway to reduce the progression of gastric cancer and reveals a new molecular mechanism for metformin in suppressing gastric cancer. Our present study provides a new insight into the mechanism and therapy for gastric cancer.


Assuntos
Biomarcadores Tumorais/metabolismo , Proteínas de Ligação a DNA/metabolismo , Glicoproteínas/metabolismo , Fosfopiruvato Hidratase/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Gástricas/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Animais , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , China , Feminino , Expressão Gênica/genética , Regulação Neoplásica da Expressão Gênica/genética , Genes Supressores de Tumor/fisiologia , Glicoproteínas/genética , Humanos , Masculino , Metformina/metabolismo , Metformina/farmacologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Oncogenes , Prognóstico , Proteínas Proto-Oncogênicas c-akt/fisiologia , Transdução de Sinais/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Ubiquitina-Proteína Ligases/metabolismo , Ubiquitinação
4.
J Phys Chem Lett ; 12(29): 6760-6766, 2021 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-34264663

RESUMO

Assessing the hardness of structural materials at elevated temperatures is experimentally and computationally challenging, yet crucial for their success. In this work, a machine-learning method was developed to determine a material's temperature-dependent hardness based on its chemical composition and crystal structure. A total of 593 Vickers hardness data collected at various temperatures were extracted from the literature and used to train an extreme gradient boosting (XGBoost) machine-learning model. Applying a combination of composition descriptors and smooth overlap of atomic positions (SOAP) structural descriptors to represent these materials resulted in outstanding accuracy (R2 = 0.91; MAE = 2.52 GPa). The model's intrinsic variance was also measured by using a bootstrap aggregating (bagging) method, and the subsequent predictions showed strong agreement with the experimental data. The capability of the trained model was finally verified by demonstrating the model's ability to discriminate polymorphs, separate the properties of similar compositions, and reproduce the high-temperature hardness of several classic structural materials.

5.
Transplant Cell Ther ; 27(9): 758.e1-758.e8, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34174470

RESUMO

Transplant-associated thrombotic microangiopathy (TA-TMA) is a serious and life-threatening complication after hematopoietic stem cell transplantation. Studies have reported that the main pathological manifestation of the disease is an endothelial injury associated with complement activation, but its molecular biological mechanisms remain unclear. Our previous studies have shown that oxidative stress may induce complement activation in TA-TMA. Nuclear factor erythroid 2-related factor 2 (Nrf2), a molecule that regulates oxidative stress, can inhibit endothelial stimulation by reactive oxygen species (ROS). We assessed Nrf2 expression in peripheral blood mononuclear cells (PBMCs) from patients with TA-TMA compared with healthy donors. Nrf2 expression, ROS accumulation, complement activation, and apoptosis were then assessed in human umbilical vein endothelial cells (HUVECs) incubated with TA-TMA plasma to identify whether complement-associated endothelial damage induced by oxidative stress occurs in TA-TMA. The protective effect of Nrf2 pathway activation on TA-TMA-induced endothelial injury was also investigated to explore a new avenue for TA-TMA prevention and treatment. In this study, peripheral blood was collected from six patients with TA-TMA, and healthy donors served as negative controls. We determined the expression of Nrf2 through in vitro and in vivo experiments and measured the level of apoptosis. We found increased expression of Nrf2 in PBMCs from patients with TA-TMA. HUVECs were then incubated with plasma from patients with TA-TMA or with plasma from healthy donors, and we found that complement 3 (C3) levels were increased in HUVECs treated with TA-TMA plasma. In contrast, total Nrf2 levels were decreased, and ROS production and apoptosis levels were increased. To determine whether complement activation and apoptosis were caused by oxidative stress, we added N-acetyl-L-cysteine to HUVECs incubated with TA-TMA plasma. As a result, ROS production, complement activation, and apoptosis levels were reduced. Finally, we upregulated Nrf2 in HUVECs by rescue experiments, and we found that activation of Nrf2 attenuated endothelial cell apoptosis and ROS production and reduced C3 and C5b-9 levels. These results suggest that oxidative stress-induced, complement activation-associated endothelial injury occurs in TA-TMA and that upregulation of Nrf2 protects endothelial cells from damage. Activation of the Nrf2 pathway may be a potential target for the treatment of complement activation-associated endothelial injury in TA-TMA.

6.
Environ Pollut ; 283: 117087, 2021 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-33894629

RESUMO

With the rapid development of science and technology, 5G technology will be widely used, and biosafety concerns about the effects of 5G radiofrequency radiation on health have been raised. Drosophila melanogaster was selected as the model organism for our study, in which a 3.5 GHz radiofrequency radiation (RF-EMR) environment was simulated at intensities of 0.1 W/m2, 1 W/m2, and 10 W/m2. The activity of parent male and offspring (F1) male flies was measured using a Drosophila activity monitoring system under short-term and long-term 3.5 GHz RF-EMR exposure. Core genes associated with heat stress, the circadian clock and neurotransmitters were detected by QRT-PCR technology, and the contents of GABA and glutamate were detected by UPLC-MS. The results show that short-term RF-EMR exposure increased the activity level and reduced the sleep duration while long-term RF-EMR exposure reduced the activity level and increased the sleep duration of F1 male flies. Under long-term RF-EMR, the expression of heat stress response-related hsp22, hsp26 and hsp70 genes was increased, the expression of circadian clock-related per, cyc, clk, cry, and tim genes was altered, the content of GABA and glutamate was reduced, and the expression levels of synthesis, transport and receptor genes were altered. In conclusion, long-term RF-EMR exposure enhances the heat stress response of offspring flies and then affects the expression of circadian clock and neurotransmitter genes, which leads to decreased activity, prolonged sleep duration, and improved sleep quality.


Assuntos
Proteínas de Drosophila , Drosophila melanogaster , Animais , Cromatografia Líquida , Ritmo Circadiano , Comunicação , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Regulação da Expressão Gênica , Masculino , Sono , Espectrometria de Massas em Tandem
7.
Sci Rep ; 11(1): 2123, 2021 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-33483577

RESUMO

Electromagnetic radiation is an important environmental factor. It has a potential threat to public health and ecological environment. However, the mechanism by which electromagnetic radiation exerts these biological effects remains unclear. In this study, the effect of Microcystis aeruginosa under electromagnetic radiation (1.8 GHz, 40 V/m) was studied by using transcriptomics. A total of 306 differentially expressed genes, including 121 upregulated and 185 downregulated genes, were obtained in this study. The differentially expressed genes were significantly enriched in the ribosome, oxidative phosphorylation and carbon fixation pathways, indicating that electromagnetic radiation may inhibit protein synthesis and affect cyanobacterial energy metabolism and photosynthesis. The total ATP synthase activity and ATP content significantly increased, whereas H+K+-ATPase activity showed no significant changes. Our results suggest that the energy metabolism pathway may respond positively to electromagnetic radiation. In the future, systematic studies on the effects of electromagnetic radiation based on different intensities, frequencies, and exposure times are warranted; to deeply understand and reveal the target and mechanism of action of electromagnetic exposure on organisms.


Assuntos
Radiação Eletromagnética , Exposição Ambiental/análise , Regulação Bacteriana da Expressão Gênica/efeitos da radiação , Microcystis/genética , Transcriptoma/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Perfilação da Expressão Gênica/métodos , Genes Bacterianos/genética , Redes e Vias Metabólicas/genética
8.
Environ Sci Pollut Res Int ; 28(15): 19202-19221, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33398727

RESUMO

Given the context of the global energy shortage and the deterioration of the ecological environment, this paper uses industrial linkage as a starting point to deeply explore the energy consumption of different sectors and its transfer characteristics. First, a dual evaluation of energy consumption in various sectors is conducted from the perspectives of production and demand to realize an integrated analysis from the energy consumption perspective. Second, based on industrial linkage, the economic input-output life cycle assessment (EIO-LCA) model is used to quantify the net transfer of energy consumption and identify the transfer of energy consumption pressure embodied in economic activities by various sectors. Finally, the energy consumption of various sectors is decomposed, and the transfer flow of energy consumption is measured to accurately quantify the degree of linkage in the energy consumption of sectors. The results show that the current direct energy consumption intensity of various sectors in China is continuously decreasing, but this decrease is insufficient to reverse the upward trend in energy consumption demand. From the perspective of production and demand, non-energy industrial sector is a key sector for reducing energy consumption, and the intersectoral linkage between the subsectors caused by product trade flows has a greater impact on the level of direct and embodied energy consumption. Direct and embodied energy consumption in the same sector can be ranked quite differently, and their levels may not be equal. Energy sector is the main net outflow sector of energy consumption, and the level of its direct energy consumption is much higher than that of its embodied energy consumption. It is worth noting that the scale of embodied energy consumption in construction industry is much higher than the scale of direct energy consumption. Construction is the sector with the greatest net inflow of energy consumed and is the main driver of the energy consumption transfer of energy sector and non-energy industrial sector.


Assuntos
Desenvolvimento Econômico , Indústrias , China
9.
Adv Mater ; 33(5): e2005112, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33274804

RESUMO

An ensemble machine-learning method is demonstrated to be capable of finding superhard materials by directly predicting the load-dependent Vickers hardness based only on the chemical composition. A total of 1062 experimentally measured load-dependent Vickers hardness data are extracted from the literature and used to train a supervised machine-learning algorithm utilizing boosting, achieving excellent accuracy (R2  = 0.97). This new model is then tested by synthesizing and measuring the load-dependent hardness of several unreported disilicides and analyzing the predicted hardness of several classic superhard materials. The trained ensemble method is then employed to screen for superhard materials by examining more than 66 000 compounds in crystal structure databases, which show that 68 known materials have a Vickers hardness ≥40 GPa at 0.5 N (applied force) and only 10 exceed this mark at 5 N. The hardness model is then combined with the data-driven phase diagram generation tool to expand the limited number of reported high hardness compounds. Eleven ternary borocarbide phase spaces are studied, and more than ten thermodynamically favorable compositions with a hardness above 40 GPa (at 0.5 N) are identified, proving this ensemble model's ability to find previously unknown materials with outstanding mechanical properties.

10.
Environ Sci Pollut Res Int ; 28(7): 8181-8204, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33052568

RESUMO

Low-carbon transition has gradually become the focus of research on environmental issues. This paper takes China's eight major economic regions as the entry point. First, carbon emissions are measured according to United Nations' baseline methodologies. Second, the stochastic nonparametric data envelope analysis (StoNED) model is used to measure energy efficiency to improve the accuracy of the measurement. Finally, considering the temporal and spatial nonstationarity of carbon emission data, this paper constructs geographically and temporally weighted regression-stochastic impacts by regression on population, affluence, and technology (GTWR-STIRPAT) model, which can accurately analyze the impact of each driving factor of carbon emissions. This paper also explores efficient emission reduction paths in conjunction with the forcing mechanism. According to the study, China's carbon emissions show a decreasing trend from coastal areas to inland areas. In addition, there are significant problems with carbon emissions in China: some regions focus on improving energy efficiency but neglect increasing energy consumption; some regions focus on industrial development but neglect long-term emission reductions. Among the driving factors, energy efficiency, foreign trade, environmental regulations, and industrial structure have the effects of spatiotemporal heterogeneity, spatial heterogeneity, and time lag on carbon emissions, respectively. Graphical abstract.


Assuntos
Desenvolvimento Econômico , Gases de Efeito Estufa , Carbono/análise , Dióxido de Carbono/análise , China , Gases de Efeito Estufa/análise
11.
Anal Methods ; 13(3): 322-326, 2021 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-33367363

RESUMO

Herein, we simply synthesized intrinsic fluorescent polydopamine nanoparticles (PDA NPs) in sodium hydroxide solution (NaOH, pH 11), and constructed a new fluorescence nanoplatform for the detection of alkaline phosphatase (ALP) using PDA NPs as an effective signal reporter. The nanoplatform was constructed by the combination of enzymatic hydrolysis of ALP to the substrate l-ascorbic acid-2-phosphate (AA2P) and the chemical redox reaction between l-ascorbic acid (AA) and mercury ion (Hg2+). The fluorescence of PDA NPs could be effectively quenched by Hg2+ through the coordination effect between Hg2+ and the functional groups on the surface of PDA NPs. However, the quenching effect was greatly inhibited by the addition of AA into the solution. Based on this point, the activity of ALP could be monitored by hydrolysis of the substrate AA2P to AA and the fluorescence output of PDA NPs. The nanoplatform exhibited high sensitivity and desirable selectivity for ALP detection. With a wide linear range of 0 to 18 U L-1, a detection limit of 0.4 U L-1 was achieved using the developed nanosensor. The proposed method could not only be used to screen the inhibitor of ALP but also be used to detect ALP activity in human serum samples successfully. Moreover, the strategy can easily be expanded to determining other kinds of enzymes participating in AA-generation reactions.

12.
Org Lett ; 22(21): 8500-8504, 2020 11 06.
Artigo em Inglês | MEDLINE | ID: mdl-33044833

RESUMO

An efficient radical transannulation reaction of pyridotriazoles with isothiocyanates and xanthate esters was developed. This method features conversion of pyridotriazoles into two N-fused heterocyclic aromatic systems-imino-thiazolopyridines and oxo-thiazolopyridine derivatives-via one-step Co(II)-catalyzed transannulation reaction proceeding via a radical mechanism. The synthetic usefulness of the developed method was illustrated in the synthesis of amino acid derivatives and further transformations of obtained reaction products.

13.
Int J Radiat Biol ; 96(12): 1633-1640, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32991227

RESUMO

PURPOSE: Safety concerns about the effects of long-term extremely low-frequency electromagnetic field (ELF-EMF) exposure on human health have been raised. To explore the effects of continuous exposure to ELF-EMF on organisms for multiple generations, we selected Caenorhabditis elegans as a model organism and conducted long-term continuous exposure studies for multiple generations under 20 °C, 50 Hz, and 3 mT ELF-EMF. MATERIALS AND METHODS: Each generation of worms was treated with ELF-EMF from the egg in the same environment. After long-term exposure to ELF-EMF, the body length of the worms was detected, and 15th generation adult worms were selected as the research object. The ATP level and ATPase were detected, and the expression levels of genes encoding ATP synthase (r53.4, hpo-18, atp-5, unc-32, atp-3) were detected by RT-PCR. In worm's antioxidant system, the level of reactive oxygen species (ROS) was detected by dichlorofluorescein staining, and the total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and catalase (CAT) activity were investigated. The expression of genes encoding superoxide dismutase (sod-1, sod-2, sod-3) was detected in adult (60 h) worms of the fifteenth generation (F15). RESULTS: These results showed that the body length of F15 worms increased significantly, ATP content increased significantly, ATP synthase activity was significantly enhanced, and the expression levels of the r53.4, hpo-18, atp-5, and atp-3 genes encoding ATPase were significantly upregulated in F15 worms. In addition, SOD activity increased significantly, and the expression levels of the sod-1, sod-2, and sod-3 genes encoding SOD were also significantly upregulated in F15 worms. CONCLUSIONS: These results indicated that continuous exposure to 50 Hz, 3 mT ELF-EMF for multiple generations can increase the body length of worms, induce the synthesis of ATP and enhance the antioxidant capacity of worms.


Assuntos
Trifosfato de Adenosina/metabolismo , Antioxidantes/metabolismo , Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/efeitos da radiação , Campos Eletromagnéticos/efeitos adversos , Adenosina Trifosfatases/metabolismo , Animais , Regulação Enzimológica da Expressão Gênica/efeitos da radiação , Superóxido Dismutase/metabolismo , Fatores de Tempo
14.
J Int Med Res ; 48(8): 300060520947883, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32812461

RESUMO

BACKGROUND: Expression levels of the cell adhesion molecule syndecan-1 (SDC1) have been shown to be inversely proportional to tumor differentiation and prognosis. However, its role in the development of gallbladder cancer (GBC) remains unclear. METHODS: We knocked down SDC1 in GBC cells by RNA interference and determined its roles in cell proliferation, apoptosis, invasion, and migration by Cell Counting Kit-8, colony-formation, flow cytometry, Hoechst 33342 staining, transwell invasion, and scratch wound assays. Expression levels of epithelial-mesenchymal transition (EMT)-related and extracellular signal-regulated kinase (ERK)/Snail pathway proteins were determined by western blotting and immunofluorescence. RESULTS: Cell proliferation, invasion, and migration were all increased in GBC cells with SDC1 knockdown, compared with cells in the blank control and negative control groups, but apoptosis was similar in all three groups. E-cadherin and ß-catenin expression levels were significantly lower and N-cadherin, vimentin, p-ERK1/2, and Snail expression were significantly higher in the SDC1 knockdown group compared with both controls, while ERK1/2 levels were similar in all groups. Reduced E-cadherin and increased vimentin levels were confirmed by immunofluorescence. CONCLUSIONS: SDC1 knockdown promotes the proliferation, invasion, and migration of GBC cells, possibly by regulating ERK/Snail signaling and inducing EMT and cancer cell invasion.


Assuntos
Transição Epitelial-Mesenquimal , Neoplasias da Vesícula Biliar , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , MAP Quinases Reguladas por Sinal Extracelular , Neoplasias da Vesícula Biliar/genética , Humanos , Invasividade Neoplásica , Sindecana-1
15.
Environ Sci Pollut Res Int ; 27(33): 41839-41855, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32700267

RESUMO

A comprehensive understanding of the relationships between greenhouse gas (GHG) emissions and industrial structure and economic growth holds great significance for China to realize the development of a green economy. This paper calculates GHG emissions based on China's energy consumption, divides the industrial structure in detail, and uses the extended Stochastic Impacts by Regression on Population, Affluence, and Technology model that is realized by PLS method and Tapio decoupling model to study the relationship of GHG emissions to industrial structure and economic growth. The results show that (1) China's total GHG emissions showed a year-on-year growth trend from 2000 to 2017. For CO2, CH4, and N2O, only N2O emission showed a significant downward trend, while CO2 and CH4 emissions showed a slow growth trend. (2) The proportions of added value of industry and construction are positively correlated with GHG emissions, while those of farming, forestry, animal husbandry, and fishery; wholesale and retail trade; transport; and accommodation and catering are negatively correlated with GHG emissions. (3) China's GHG emissions and overall economic growth are in a decoupling state, but in the energy field, N2O emission reduction control has the best effect. Additionally, the overall economic growth of China's industrial sector and GHG emissions have experienced the process of decoupling-link-negative decoupling-link-decoupling. Graphical abstract.


Assuntos
Gases de Efeito Estufa , Dióxido de Carbono/análise , China , Desenvolvimento Econômico , Efeito Estufa , Indústrias
16.
J Exp Clin Cancer Res ; 39(1): 138, 2020 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-32682428

RESUMO

The epigenetic abnormality is generally accepted as the key to cancer initiation. Epigenetics that ensure the somatic inheritance of differentiated state is defined as a crucial factor influencing malignant phenotype without altering genotype. Histone modification is one such alteration playing an essential role in tumor formation, progression, and resistance to treatment. Notably, changes in histone acetylation have been strongly linked to gene expression, cell cycle, and carcinogenesis. The balance of two types of enzyme, histone acetyltransferases (HATs) and histone deacetylases (HDACs), determines the stage of histone acetylation and then the architecture of chromatin. Changes in chromatin structure result in transcriptional dysregulation of genes that are involved in cell-cycle progression, differentiation, apoptosis, and so on. Recently, HDAC inhibitors (HDACis) are identified as novel agents to keep this balance, leading to numerous researches on it for more effective strategies against cancers, including glioblastoma (GBM). This review elaborated influences on gene expression and tumorigenesis by acetylation and the antitumor mechanism of HDACis. Besdes, we outlined the preclinical and clinical advancement of HDACis in GBM as monotherapies and combination therapies.


Assuntos
Glioblastoma/tratamento farmacológico , Inibidores de Histona Desacetilases/uso terapêutico , Histona Desacetilases/química , Processamento de Proteína Pós-Traducional , Acetilação , Animais , Glioblastoma/enzimologia , Glioblastoma/patologia , Humanos
17.
Leuk Lymphoma ; 61(11): 2682-2691, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32605403

RESUMO

Increasing evidence has revealed that plasma fibrinogen levels may serve as prognostic indicators in patients with acute myeloid leukemia (AML), yet the exact association is still elusive. We conducted a systematic review and meta-analysis of all available studies concerning the relationship between plasma fibrinogen level and survival in AML patients. The pooled hazard ratio (HR) and 95% confidence intervals (CIs) for overall survival (OS) were calculated to evaluate the effect. A random-effect model was applied and the robustness of the pooled results was confirmed by subgroup and sensitivity analysis. A total of 9 studies were eligible to assess the association between plasma fibrinogen level and prognosis in AML. Among these investigations above, 5 studies adopted OS as their outcome indicator and were selected for the final meta-analysis. The pooled result suggested that plasma fibrinogen level was significantly relevant to increased mortality risk in AML patients (HR = 1.21, 95% CI: 1.01-1.44, p = .000, I2=85.4%). In conclusion, high plasma fibrinogen level may independently predict worse OS in patients with AML.


Assuntos
Leucemia Mieloide Aguda , Fibrinogênio , Humanos , Leucemia Mieloide Aguda/diagnóstico , Plasma , Prognóstico , Modelos de Riscos Proporcionais
18.
Medicine (Baltimore) ; 99(14): e19605, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32243383

RESUMO

Patients suffering from extremely medial clavicle fractures combined with distinct displacement generally need surgical intervention. Double-plate fixation is a widely applied technique in the treatment of distal radius fracture, which has been reported to fix lateral clavicle fracture as well. This study reveals the effect of double-plate fixation as an innovative procedure in the treatment of extremely medial clavicle fractures for the first time.Nine patients complaint of extremely medial clavicle fracture were enrolled in this research from May 2017 to March 2019. Patients were operated with an open reduction and internal fixation using the double-plate technique. Postoperative x-ray was taken regularly to observe the fracture healing at each visit, and the related complications were also recorded. The rating score systems of Constant Murley score of treated shoulder and contralateral shoulder, ROWE score as well as American Shoulder and Elbow Surgeons (ASES) were evaluated to comment on the postoperative shoulder joint function.All patients achieved postoperative fracture healing with no complications. Only 1 patient complained of slight restriction, 2 patients complained of pain during overhead work, and another patient was found with plate breakage. Meanwhile, the Constant Murley scores of treated and contralateral shoulder were 94.1 and 98.5 points, respectively, indicating the similar shoulder function. Furthermore, the ROWE and ASES scores of the involved shoulder were 96.7 and 96.3 points at average, respectively.It is the first time to introduce the surgical technique of vertical double-plate fixation implied in stable fixation of extremely medial clavicle fractures, which could provide the surgeons with an alternative method for this type of fracture.


Assuntos
Placas Ósseas , Clavícula/lesões , Fixação Interna de Fraturas/métodos , Fraturas Ósseas/cirurgia , Redução Aberta/métodos , Adulto , Idoso , Clavícula/cirurgia , Estudos de Viabilidade , Feminino , Consolidação da Fratura , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
20.
Food Chem Toxicol ; 138: 111245, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32145355

RESUMO

It appears that electronic cigarettes (EC) are a less harmful alternative to conventional cigarette (CC) smoking, as they generate substantially lower levels of harmful carcinogens and other toxic compounds. Thus, switching from CC to EC may be beneficial for smokers. However, recent accounts of EC- or vaping-associated lung injury (EVALI) has raised concerns regarding their adverse health effects. Additionally, the increasing popularity of EC among vulnerable populations, such as adolescents and pregnant women, calls for further EC safety evaluation. In this state-of-the-art review, we provide an update on recent findings regarding the neurological effects induced by EC exposure. Moreover, we discuss possible neurotoxic effects of nicotine and numerous other chemicals which are inherent both to e-liquids and EC aerosols. We conclude that in recognizing pertinent issues associated with EC usage, both government and scientific researchers must address this public health issue with utmost urgency.


Assuntos
Sistemas Eletrônicos de Liberação de Nicotina , Síndromes Neurotóxicas , Nicotina/toxicidade , Adolescente , Aerossóis/efeitos adversos , Animais , Encéfalo/efeitos dos fármacos , Feminino , Radicais Livres/toxicidade , Humanos , Metais Pesados/toxicidade , Nanopartículas/toxicidade , Gravidez , Saúde Pública , Fumantes , Fumar , Oligoelementos/toxicidade , Vaping/efeitos adversos
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