Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.003
Filtrar
1.
Zhonghua Gan Zang Bing Za Zhi ; 29(10): 972-976, 2021 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-34814391

RESUMO

Objective: To investigate the efficacy of transjugular intrahepatic portosystemic shunt (TIPS) using viabahn stent in patients with Budd-Chiari syndrome (BCS) with extensive hepatic vein occlusion. Methods: Technical success rates, portal pressure gradient (PPG), complications and shunt patency rate of 25 cases with BCS with extensive hepatic vein occlusion treated by TIPS using viabahn stent were retrospectively analyzed. Color Doppler ultrasound was used for postoperative follow-up. Patency rates were evaluated by Kaplan-Meier curve. Results: The technical success rate of TIPS was 100%. Mean PPG decreased from (22.7 ± 6.2) mmHg preoperatively to (9.7 ± 2.6) mmHg postoperatively (t = 9.58, P < 0.05). Hepatic encephalopathy incidence was 8.0% (2/25), and there were no complications such as intra-abdominal hemorrhage. Twenty-five cases were followed up for 1 to 39 ( mean 19.0 ± 11.9) months, and one patient died of liver failure three months after interventional treatment. The primary patency rates at 1, 2, and 3 years after interventional treatment were 91.3%, 91.3%, and 78.2%, respectively, while the repatency rates were 100%, 92.9%, and 92.9%, respectively. Conclusion: TIPS using viabahn stent can bring better short-term and mid-term curative efficacy in treatment of BCS with extensive hepatic vein occlusion.

2.
Zhonghua Liu Xing Bing Xue Za Zhi ; 42(9): 1615-1620, 2021 Sep 10.
Artigo em Chinês | MEDLINE | ID: mdl-34814592

RESUMO

Objective: To investigate the incidence and determinants of vaccine hesitancy towards national immunization program in China and understand the current status of parents' hesitancy to different vaccines used in national immunization program. Methods: A cross-sectional survey was conducted in Beijing, Sichuan and Gansu. The methods of proportional probability sampling and convenience sampling were used to select the eligible study subjects for questionnaire surveys. Results: A total of 3 592 parents were enrolled in the study, in whom 38.22% fully accepted all the vaccines, 59.35% agreed to let their children to receive all the vaccines but showed slight concern, and 2.42% had hesitancy to the vaccines. The vaccine with the most hesitancy was polio vaccine (0.89%), followed by diphtheria pertussis tetanus vaccine (0.70%) and hepatitis A vaccine (0.64%). The dominant reason for vaccine hesitancy was the risk-benefit perception of vaccination (31.03%), followed by the low awareness of the parents (21.84%) and the inconvenience caused by distance and time (21.84%). Conclusions: The incidence of vaccine hesitancy towards national immunization program was low in parents in China, but over 50% of the parents showed concern to the vaccines. It is essential to improve the service quality of national immunization program and strengthen the health education about the vaccination to reduce the incidence of vaccine hesitancy in parents.

3.
Artigo em Chinês | MEDLINE | ID: mdl-34666441

RESUMO

Objective: To evaluate the auditory efficacy and subjective satisfaction of adhesive bone conduction hearing aid in children with unilateral congenital aural atresia (UCAA). Methods: Ten subjects (5 males and 5 females) diagnosed with UCAA with an average age of 8.3 years old (ranged from 5 to 15) were included in Beijing Tongren Hospital, Capital Medical University from January to August 2019. The free sound field hearing threshold, word recognition score in quiet, speech reception threshold in noise and sound localization ability (results were measured by RMS error) tests were performed in unaided and aided situation, respectively. Subjective satisfaction questionnaires were also distributed to subjects. Paired t test and Wilcoxon signed rank test were used as statistical analysis methods. Results: The average hearing threshold in aided condition was improved by (21.9±4.4) dB (t=15.8,P<0.05). Speech recognition abilities were generally improved both under quiet and noise (P<0.05);however, when the binaural summation, squelch and head shadow effects were analyzed respectively, the binaural squelch effect was not statistically improved (P>0.05), while the other effects were improved in aided condition (P<0.05). In sound localization test, there was no significant difference of the RMS error value between the unaided and aided situation (P>0.05). The subjects got high satisfaction rates in three subjective questionnaires. Conclusion: The adhesive bone conduction hearing aid can provide significant audiological benefit for children with UCAA as well as raising the quality of their life.


Assuntos
Auxiliares de Audição , Percepção da Fala , Adesivos , Adolescente , Condução Óssea , Criança , Pré-Escolar , Feminino , Perda Auditiva Condutiva , Humanos , Masculino , Resultado do Tratamento
4.
Zhonghua Yu Fang Yi Xue Za Zhi ; 55(10): 1240-1244, 2021 Oct 06.
Artigo em Chinês | MEDLINE | ID: mdl-34706511

RESUMO

An epidemiological investigation was carried out on a local cluster of outbreak caused by imported cases of Coronavirus Disease 2019 (COVID-19) in rural areas of Chengdu in December 2020, to find out the source of infection and the chain of transmission. According to Prevention and Control Protocol for COVID-19 (Version 7), field epidemiological investigation was adopted, combined with big data technology, video image investigation, gene sequencing and other methods to carry out investigation into COVID-19 cases and infections source tracing, analyze the epidemiological association, and map the chain of transmission. From December 7 to 17, 2020, 13 local COVID-19 confirmed cases and 1 asymptomatic case were diagnosed in Chengdu, of which 12 cases (85.71%) had a history of residence and activity in the village courtyard of Taiping (TP), Pidu (P) District, Chengdu. From November 8, 2020 to November 28, 2020, a group of inbound people form Nepal were transferred to the designated entry personnel quarantine hotel of P District which was adjacent to the TP village. During quarantine, there were 5 cases who tested positive for COVID-19. Through gene sequencing alignment, genes of local cases and Nepalese imported cases from the same period are homologous, all belong to the lineage of L2.2.3 (B.1.36 according to Pangolin lineage typing method). According to the results of field epidemiological investigation and gene sequencing analysis, the index case was most likely infected by contact with household waste of quarantine site. Under the situation of normalization prevention and control of COVID-19, sentinel monitoring of fever clinics in primary medical institutions is the key to early detection of the epidemic. The multi-department joint epidemiological investigation and the application of gene technology are the core links of the investigation and traceability of modern infectious diseases. The allocation of public health resources in rural areas needs to be strengthened. We need to improve the capacity for early surveillance and early warning of the epidemic in rural areas.


Assuntos
COVID-19 , Epidemias , Surtos de Doenças , Humanos , Quarentena , SARS-CoV-2
5.
Mater Today Bio ; 12: 100142, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34647005

RESUMO

Bone defects associated with soft tissue injuries are an important cause of deformity that threatens people's health and quality of life. Although bone substitutes have been extensively explored, effective biomaterials that can coordinate early inflammation regulation and subsequent repair events are still lacking. We prepared a spatial form periosteal bone extracellular matrix (ECM) scaffold, which has advantages in terms of low immunogenicity, good retention of bioactive ingredients, and a natural spatial structure. The periosteal bone ECM scaffold with the relatively low-stiffness periosteum (41.6 ± 3.7 kPa) could inhibit iNOS and IL-1ß expression, which might be related to actin-mediated YAP translocation. It also helped to promote CD206 expression with the potential influence of proteins related to immune regulation. Moreover, the scaffold combined the excellent properties of decalcified bone and periosteum, promoted the formation of blood vessels, and good osteogenic differentiation (RUNX2, Col 1α1, ALP, OPN, and OCN), and achieved good repair of a cranial defect in rats. This scaffold, with its natural structural and biological advantages, provides a new idea for bone healing treatment that is aligned with bone physiology.

6.
Ann Oncol ; 32(12): 1626-1636, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34606929

RESUMO

BACKGROUND: Tumor mutational burden (TMB) measurements aid in identifying patients who are likely to benefit from immunotherapy; however, there is empirical variability across panel assays and factors contributing to this variability have not been comprehensively investigated. Identifying sources of variability can help facilitate comparability across different panel assays, which may aid in broader adoption of panel assays and development of clinical applications. MATERIALS AND METHODS: Twenty-nine tumor samples and 10 human-derived cell lines were processed and distributed to 16 laboratories; each used their own bioinformatics pipelines to calculate TMB and compare to whole exome results. Additionally, theoretical positive percent agreement (PPA) and negative percent agreement (NPA) of TMB were estimated. The impact of filtering pathogenic and germline variants on TMB estimates was assessed. Calibration curves specific to each panel assay were developed to facilitate translation of panel TMB values to whole exome sequencing (WES) TMB values. RESULTS: Panel sizes >667 Kb are necessary to maintain adequate PPA and NPA for calling TMB high versus TMB low across the range of cut-offs used in practice. Failure to filter out pathogenic variants when estimating panel TMB resulted in overestimating TMB relative to WES for all assays. Filtering out potential germline variants at >0% population minor allele frequency resulted in the strongest correlation to WES TMB. Application of a calibration approach derived from The Cancer Genome Atlas data, tailored to each panel assay, reduced the spread of panel TMB values around the WES TMB as reflected in lower root mean squared error (RMSE) for 26/29 (90%) of the clinical samples. CONCLUSIONS: Estimation of TMB varies across different panels, with panel size, gene content, and bioinformatics pipelines contributing to empirical variability. Statistical calibration can achieve more consistent results across panels and allows for comparison of TMB values across various panel assays. To promote reproducibility and comparability across assays, a software tool was developed and made publicly available.

7.
Zhonghua Shao Shang Za Zhi ; 37(10): 953-958, 2021 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-34689465

RESUMO

Objective: To investigate the application effects of free transplantation of autologous omentum in treating maxillofacial infection complicated with complex sinus tract. Methods: The retrospective observational study method was used. Four patients with maxillofacial infection complicated with complex sinus tract were admitted to Department of Burns and Plastic Surgery of the Second Affiliated Hospital of Air Force Medical University from July 2017 to the December 2019, including 3 males and 1 female aged 36-60 years. Preoperative facial computed tomography (CT) was performed on patients for calculating the volume of sinus tract. During the operation, the sinus tract was thoroughly debrided, and the volume of the tissue defect was about 5 cm×3 cm×2 cm-10 cm×5 cm×3 cm after debridement. The tissue defect area was filled with omentum of 100-300 mL which was cut under laparoscopy. The artery and vein on the right side of the omentum were reserved as the vascular pedicle of the donor area, which were anastomosed with the facial artery and external jugular vein of the recipient area. The survival of omentum, and the occurrences of reinfection and complication were observed after operation, respectively. On the 10th day and in 1 month after the operation, the blood supply of omentum was examined by colored Doppler ultrasound and CT angiography, and the filling of tissue defect area was examined by head and face CT. During follow-up after the operation, the recoveries of face appearance and function and scar hyperplasia in the donor area. Results: The transplanted omentums in 4 patients survived after the operation with no reinfection and complication. On the 10th day and in 1 month after the operation, the transplanted omentums had good blood supply, and the filled area with omentum was in good shape, without formation of dead cavity. During follow-up of 6-10 months after surgery, the appearance and function of face recovered well, and there was no obvious scar hyperplasia in the donor area. Conclusions: After free transplantation of omentum in treating maxillofacial infection with complex sinus tract, the patients have good facial appearance and function, and the application of laparoscopy results in little damage to the patients and quick postoperative recovery.


Assuntos
Queimaduras , Procedimentos Cirúrgicos Reconstrutivos , Lesões dos Tecidos Moles , Queimaduras/cirurgia , Feminino , Humanos , Masculino , Omento/cirurgia , Transplante de Pele , Lesões dos Tecidos Moles/cirurgia , Resultado do Tratamento
8.
Int J Radiat Oncol Biol Phys ; 111(3S): e229, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34700964

RESUMO

PURPOSE/OBJECTIVE(S): To investigate the effect of circadian clock gene BMAL1 on proliferation and cell cycle of Nasopharyngeal Carcinoma(NPC) CNE1 cells with different expression status, compare the expression level of pathway related proteins before and after radiotherapy, to further understand the mechanism of BMAL1 gene regulating the biological behavior of NPC cells, aim to explore the mechanism of BMAL1 gene regulating the expression of cell cycle related proteins through ATM/ATR pathway at the molecular level. MATERIALS/METHODS: Constructed CNE1 cell lines with BMAL1 gene overexpression group, control group, inhibitor group, blank group. Using Western blot (WB) to detect the overexpression of BMAL1 gene at protein level and the expression of ATM/ATR pathway related proteins. The absorbance of cells with the same number of plates was determined by a cell counting kit for 6 consecutive days. The effect of BMAL1 gene on the proliferation rate of CNE1 was analyzed. The effect of BMAL1 gene on cell cycle of CNE1 was studied by flow cytometry. Co-immunoprecipitation (co-IP) was used to detect the interaction between BMAL1 gene and ATM/ATR checkpoints. RESULTS: WB showed BMAL1 gene had a higher expression in the overexpression group. In the BMAL1 gene overexpression group, the absorbance values of CNE1 cells were lower at 1 to 6 days, the proportion of G0/G1 phase cells was higher, the relative protein expression of P21,P27 were higher. Co-IP confirmed the interaction between BMAL1 gene and the checkpoints of ATM/ATR pathway before and after radiotherapy. The protein expression of upstream genes of ATM/ATR pathway in the overexpression group was significantly higher before radiotherapy (P < 0.05). The protein expression of the upstream genes of ATM/ATR pathway increased significantly, while the expression of the downstream genes decreased significantly after radiotherapy. Comparing with the overexpression group and inhibitor group showed when the ATM/ATR pathway blocked by inhibitor KU55933, the expression of ATM/ATR pathway upstream genes decreased, and the expression of downstream genes increased. The expression of p-chk1 in blank group, control group and overexpression group after radiotherapy was significantly higher than before, all the difference were statistically significant. CONCLUSION: The circadian clock gene BMAL1 inhibits the proliferation of NPC cell line CNE1, arrests cell cycle in the G0/G1 phase by up-regulate the expression of P21,P27 and P53.The response of ATM/ATR pathway could be activated after radiotherapy, the overexpression of Bmal1 gene could up-regulate the expression of ATM/ATR pathway upstream genes such as p-chk1 and p-chk2,then inhibited the activity of downstream Cyclin-CDK complexes by phosphorylated CDC25A and CDC25C,finally achieved the function of blocking cell cycle after radiotherapy.

9.
Int J Radiat Oncol Biol Phys ; 111(3S): e232, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34700973

RESUMO

PURPOSE/OBJECTIVE(S): Host immunity has a significant impact on radiotherapy (RT) outcomes in cancer. We have previously demonstrated that Indoleamine 2, 3-dioxygenase (IDO), a known immunoinhibitory kinase, predicts the survival of patients with stage I/II after RT alone and stage III non-small cell lung cancer (NSCLC) after chemoradiation. We hypothesize that the IDO activity levels in the blood also change in patients with stage IV NSCLC following radiotherapy. MATERIALS/METHODS: This was part of a prospective study of circulating immune biomarkers for prognosis and prediction. Newly diagnosed or recurrent stage IV NSCLC patients were eligible. Blood samples were obtained before and after RT start and platelet-poor plasma samples were used for the kynurenine (Kyn) and tryptophan (Trp) measurement by high-performance liquid chromatography. Kyn and Trp was detected on a UV/vis channel at 360 nm and 285 nm, respectively, with more than 95% re-productivity. IDO activity was defined as the ratio between Kyn and Trp in synchronization. Student T-test was applied for group comparison. Data are presented as mean and 95% confidence interval (CI). P value < 0.05 was considered as statistical significance. RESULTS: Between July 2019 and Dec 2020, a total of 54 patients with stage IV NSCLC treated with radiotherapy was recruited. RT dose ranged from 20-60 Gray in 5-30 fractions, covering thoracic, spines or brain. Higher concentration of Kyn (mean = 1.88, CI = 1.48-2.27 mM) was detected before RT, compared with the Kyn concentration (mean = 1.20, CI = 0.70-1.70 mM) after RT (P = 0.052). The mean concentration of Trp was 20.81 (CI = 17.01-24.62) mM before RT, compared with 19.01 (CI = 17.03-21.00) mM after RT with no significant change (P = 0.982). In addition, the IDO activity (mean = 0.11, CI = 0.08-0.14) before RT was higher than the IDO activity after RT (mean = 0.08, CI = 0.05-0.10) with P = 0.130. Furthermore, a subgroup analysis of the IDO activity in patients with distant metastasis (n = 31) was conducted. The mean concentration of Kyn was significantly decreased from 1.78 (CI = 1.67-2.67) mM before RT to 0.68 (CI = 0.28-2.09) mM after RT (P = 0.028). The mean concentration of Trp was 18.12 (CI = 15.39-20.84) mM before RT versus 18.64 (CI = 14.41-22.86) mM after RT (P = 0.847). Besides, the IDO activity was also reduced from 0.15 (CI = 0.09-0.20) before RT to 0.07 (CI = 0.01-0.13) after RT (P = 0.051). CONCLUSION: This study reveals that the plasma kynurenine and IDO activity is reduced after RT, suggesting that potential roles of RT in releasing immune suppression status of the host that can be simply tested by IDO activity in patients with stage IV NSCLC.

10.
Int J Radiat Oncol Biol Phys ; 111(3S): e233, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34700976

RESUMO

PURPOSE/OBJECTIVE(S): Thoracic radiotherapy combined with PD-1 inhibitor can promote the activation of T cell and improve the treatment outcomes of patients with chest cancer. However, it also increases the risk of myocardial injury. A mouse model was used to observe the effects of PD-1 inhibitors on myocardial immune microenvironment and radiation induced injury, and to explore the potential mechanism of PD-1 inhibitors aggravating myocardial injury using. MATERIALS/METHODS: Totally 20 C57BL/6 mice were randomly divided into 4 groups, including group A as normal control, group B receiving intraperitoneally injected with anti-PD-1 antibody, group C receiving full thoracic x-ray irradiation with 15 Gy in one fraction, D group receiving anti-PD1 as the same as B group and full thoracic x-ray irradiation as C group. At 1 months after irradiation, mice were killed under anesthesia. Hematoxylin-eosin staining and Mason staining were used to observe myocardial injury and fibrosis. The levels of CD3+, CD3+CD4+, CD3+CD8+ lymphocyte and cytokines (IL-4, IL-6, IL-17A, TNF-α, TGF-ß1, INF-γ) in myocardium were detected by flow cytometry. The apoptosis rate of myocardial cells was detected by TUNEL assay. RESULTS: Mason staining showed that no obvious myocardial fibrosis in group B, and collagen fibers were distributed in the myocardial interstitium in groups C and D. The results of semi-quantitative analysis showed that the myocardial collagen volume fraction (CVF) of each group A, B, C and D were (1.97 ± 0.36)%, (2.83 ± 1.03)%, (5.39 ± 0.77)% and (7.72 ± 1.43)%, respectively. The CVF of group A was similar to that of group B (P = 0.314). There were statistically significant differences in CVF among other groups (P < 0.05). Compared with group A, the absolute value and percentage of CD3+T lymphocytes in groups B, C and D were increased (P < 0.01), and those in group D were higher than those in group B and C (P < 0.01). The absolute value and percentage of CD3+CD4+T lymphocytes in groups A, C, B and D were similar (P > 0.05). The absolute value and percentage of CD3+CD8+T lymphocytes in group D were higher than those in groups A, B and C (P < 0.001). The levels of IL-6, IL-17A and TGF-ß1 in group D were higher than those in groups A, B and C (P < 0.001). The apoptotic index of groups A, B, C and D increased gradually, and the difference of apoptotic index among all groups was statistically significant (P < 0.001). CONCLUSION: PD-1 inhibitors can aggravate radiation-induced myocardial injury by promoting myocardial immunoinflammatory response.

11.
Int J Radiat Oncol Biol Phys ; 111(3S): e245-e246, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701004

RESUMO

PURPOSE/OBJECTIVE(S): Recombinant human endostatin (rh-ES) has been found to cause heart damage in clinical use. The heart tissue can be irradiated inevitably while radiation therapy for chest tumors and may result in radiation damage. The regulation of myocardial fibrosis may be related to TGF-ß1, Smad2/3, and CTGF signaling pathways. At present, whether targeted drug therapy combined with radiotherapy will increase the damage of the heart is a subject worth studying. MATERIALS/METHODS: The experimental animals were randomly divided into 25 Gy irradiation group (5 Gy/ time × 5 days), rh-ES 6mg/kg group, rh-ES 12mg/kg group, 25 Gy irradiation + rh-ES 6mg/kg group, 25 Gy irradiation + rh-ES 12mg/kg group and blank control group, with 15 in each group. rh-ES was injected intraperitoneally for 14 consecutive days. Five rats in each group were sacrificed and the cardiac tissues were extracted for HE staining, Masson staining and transmission electron microscopy at 1, 3 and 6 months after irradiation and rh-ES rejection. MTT was used to detect the value of the IC50 rh-ES to cardiomyocytes with different concentrations of rh-ES. The cardiomyocytes with stable knockdown of TGF-ß1 is grouped, the irradiation dose is 10 Gy. According to cardiomyocytes trial were divided into four group, the blank control group (without any treatment), the irradiation group (irradiation + TGF-ß1 gene knockdown), the rh-ES group (rh-ES + TGF-ß1 gene knockdown), the combined group (irradiation, rh-ES and TGF-ß1 gene knockdown), flow cytometry was used to detect cardiomyocytes apoptosis, qPCR and Western Blot were used to detect the expression of TGF-ß1/Smad2. Smad3/CTGF gene and protein. RESULTS: In vivo experiments on the heart of rats showed that a little inflammatory cell infiltration was observed under light microscope at 1 month after radiation and rh-ES treatment, and the inflammatory cell infiltration was obvious with fibrosis changes at 3 months, and the fibrosis changes were aggravated 6 months later. rh-ES alone caused slight injury to rats (no change under light microscope, but some mitochondria swelling and cristae missing under electron microscope). rh-ES and radiation treatment increased the damage of myocardial tissues in rats to some extent, and the process of radiation-induced myocardial fibrosis lasted at least 6 months after radiotherapy. rh-ES can aggravate the process of myocardial fibrosis after radiation injury, but has no toxic effect on normal myocardial tissue. The IC50 value of rh-ES on rat cardiomyocytes was 529µg/ mL in vitro experiment. qPCR and Western Blot results showed that TGF-ß1, Smad2/Smad3, CTGF gene and protein expressions were up-regulated in rat cardiomyocytes after rh-ES and irradiation treatment. CONCLUSION: The TGF-ß1, Smad2/Smad3 and CTGF signaling pathways may be the common signaling pathways of myocardial fibrosis injury induced by rh-ES combined with radiation, which may be the target for the treatment of myocardial fibrosis by radiation.

12.
Int J Radiat Oncol Biol Phys ; 111(3S): e251, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701015

RESUMO

PURPOSE/OBJECTIVE(S): BMAL1 is a core biological clock gene, which is expressed rhythmically in a variety of tumor cells, and is related to cancer cell proliferation and chemotherapy sensitivity. Radiotherapy is the main treatment for nasopharyngeal carcinoma (NPC). However, the role of baml1 in radiotherapy for nasopharyngeal carcinoma is unclear. MATERIALS/METHODS: We used 5 types of nasopharyngeal carcinoma cells (CNE1, CNE2, HONE1, C666-1, 5-8F) and normal nasopharyngeal epithelial cells NP69 as the research objects, and detected the transcription level changes of BMAL1 mRNA within 24 hours by RT-PCR. Furthermore, after overexpression and knockdown of BMAL1 in nasopharyngeal carcinoma CNE1 cells and transplanted tumors in nude mice, the effects of BMAL1 on the proliferation of CNE1 cells and subcutaneous transplanted tumors and the changes after radiotherapy were observed. Finally, RT-PCR and Western Blot were used to detect BMAL1, P53, P21 gene and protein expression in tumor tissues. RESULTS: The results suggest that the expression level of the circadian clock gene BMAL1 in NPC cell lines and NP69 cells fluctuates with time. Overexpression of BMAL1 can inhibit the proliferation of NPC cells, while knockdown promotes the proliferation of NPC cells. In the xenograft model, we observed that up-regulation of BMAL1 inhibited tumor growth and enhanced the sensitivity of NPC cells to radiotherapy, accompanied by increased expression of P53 and P21. Down-regulating BMAL1 promotes tumor growth, reduces the sensitivity of radiotherapy, and decreases the expression of P53 and P21. CONCLUSION: BMAL1 showed rhythmic expression in NPC cells and NP69. BMAL1 plays a tumor suppressor effect in NPC, and can increase the sensitivity of NPC to radiotherapy, which may be related to the up-regulation of P53 and P21 protein expression. It is speculated that BMAL1 may become a new prognostic biomarker and new therapeutic target for NPC.

13.
Int J Radiat Oncol Biol Phys ; 111(3S): e258, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701031

RESUMO

PURPOSE/OBJECTIVE(S): Radiotherapy (RT) as a traditional treatment for Breast cancer (BC) not only could induce local tumor cell death but also modify the tumor microenvironment to engage antitumor immune response. High level of tumor infiltrating lymphocytes, especially CD8, T helper cells and dendritic cells, have great correlation with pathological complete response in BC patients. However, there is no systematic report on the dynamic changes of circulating immune cells in BC treated with RT. We detected the systemic lymphocyte subsets in the peripheral blood before, during, and after RT of BC patients to evaluate the effects of RT on different lymphocyte subtypes. MATERIALS/METHODS: BC patients treated with RT were recruited to a prospective study. Blood samples were obtained EDTA coated tubes and then centrifuged for white blood cell. The antibodies including CD11b, CD45, CD19, CD3, CD56, CD4, CD8a, CD133, HLA-DR, and FOXP3 were used to identify corresponding cell subtypes. Finally, the percentage of Lymphocytes, MDSC, DC, B cells, T cells, Treg, CD8+ T cells, CD4+ T cells, NK cells, and NKT were analyzed using flow cytometry software. The paired T-test was used to compare and analyze each cell subpopulation from Pre, Dur and End of RT. P < 0.05 was considered to be significant. RESULTS: Between July 2019 and January 2020, a total of 50 BC patients treated with RT that signed consent forms and sampled at three time points (Pre, Dur, End of RT) were enrolled in this study. After RT, the ratio of CD8/CD4 (Pre vs. End mean:0.73 vs. 0.60, P = 0.04) were significantly decreased while Tregs (Pre vs. End mean:2.03 vs. 2.68, P = 0.04) were increased. Analyzing the changes of myeloid Cells, DC were shown a significant gradual increase trend (Pre vs. End mean:0.23 vs. 0.37, P < 0.0001) from baseline (mean:0.23) to during (mean: 0.33) and end (0.37) of RT. Further explore the dynamic changes of lymphocytes subtypes before and after RT under each clinical stage. In stage III patients, CD4 cells (Pre vs. Dur mean:47.62 vs. 55.64, P = 0.03) and Tregs (Pre vs. Dur mean:1.83 vs. 3.37, P = 0.01) were significantly increased during RT treatment. Whereas there were drastically reduced in CD8 cells during (Pre vs. Dur mean:36.21 vs. 30.84, P = 0.003) and after (Pre vs. End mean:36.21 vs. 29.23, P = 0.03) RT treatment. After RT treatment for stage III patients, DC were shown a significant increase trend (Pre vs. Dur mean:0.25 vs. 0.37, P = 0.02; Pre vs. End mean: 0.24 vs. 0.48, P = 0.008). For other stages, there is no significant change in immune cell subsets before and after RT. CONCLUSION: Different immune cell subtypes have different sensitivity to radiotherapy. Overall, RT could induce CD8/CD4 ratio decrease, Treg and DC increase. Interestingly, Stage III Breast cancer patients are more sensitive to RT, and their immune status changes significantly during RT treatment. Although these findings need to be verified for a larger sample size, the preliminary results provide instructions for subsequent personalized and precise treatment for different individuals.

14.
Int J Radiat Oncol Biol Phys ; 111(3S): e385, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701328

RESUMO

PURPOSE/OBJECTIVE(S): The effect on immediate effect, acute toxicity, lymphocyte immune function and EBVDNA clearance rate in patients with locally advanced nasopharyngeal carcinoma treated by TPF induction chemotherapy combined with cisplatin concurrent radiochemotherapy or PF adjuvant chemotherapy combined with cisplatin concurrent radiochemotherapy (these two therapeutic regimens fall under Class 2A evidence in the 2019 NCCN guideline) is investigated to seek a treatment method with high-efficiency and low-toxicity or equivalent and low-toxicity. MATERIALS/METHODS: Seventy-two patients diagnosed with locally advanced nasopharyngeal carcinoma from June 2018 to September 2019 were randomly divided into two groups - the TPF induction chemotherapy (experimental group) and the PF adjuvant chemotherapy (control group). The experimental group was treated with 3 cycles of TPF induction chemotherapy + cisplatin concurrent radiochemotherapy, while the control group received cisplatin concurrent radiochemotherapy + 3 cycles of PF adjuvant chemotherapy. RESULTS: In the aspect of the immediate effect, both groups had a high effective rate (CR+PR)% (91.9% vs. 93.9%, χ2 value = 0.740, P > 0.05). The CR% of the control group was superior to that of the experimental group (54.5% vs. 18.9%, χ2 value = 9.643, P < 0.05). In terms of acute toxicities, the incidence of side effects like white blood cells, neutrophils, and diarrhea in the experimental group was higher than those in the control group, with the difference statistically significant (P < 0.05), while there was no statistical difference in the incidence of hemoglobin, platelets, ALT/AST, nausea, vomiting, constipation and oral mucositis between the two groups (P > 0.05). The experimental group prevailed over the control group in CD4+/CD8+ and CD4+, while CD4+ was statistically different between the two groups (P < 0.05). After treatment, the comparison of the EBVDNA clearance rate in EBVDNA-positive patients before treatment in two groups showed that EBVDNA clearance of the experimental group was more effective than that of the control group (68.8% vs. 54.2%, χ2 value = 1.244, P > 0.05), and upon treatment, compared with the clearance of EBVDNA positive high expression before treatment in both groups, the experimental group also had a higher trend (63.6% vs. 37.5%, χ2 value = 1.269, P > 0.05), but the difference was not statistically significant in two groups. CONCLUSION: The two groups possessed the same immediate effective rate (CR+PR) %. The PF adjuvant group had less acute toxicity, while the TPF induction group had a better trend in improving lymphocyte immune function and EBVDNA clearance.

15.
Int J Radiat Oncol Biol Phys ; 111(3S): e530, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701676

RESUMO

PURPOSE/OBJECTIVE(S): Synthetic CT (sCT) generation with MR image plays an essential role in MR-only radiotherapy, which can be used for electron density estimation and dose calculation in treatment planning. Currently, the reliability of deep learning-based methods for sCT generation depends on two aspects: the MR-CT registration errors, which can eliminate by a cycle-consistent generative adversarial network (CycleGAN) with unpaired training data, and the different anatomy information and contract divergence of various tissue in different sequences MRI which will significantly affect the nonlinear mapping of MR-CT. The purpose of this study is to investigate the effect of different sequence MRI by evaluating the image quality of corresponded sCTs. MATERIALS/METHODS: Multi-sequence MR images (T1, T2, T1C) and plan CT images of 151 patients with nasopharyngeal carcinoma were acquired on the same day in the radiation treatment position. 126 cases were randomly selected as the training set with the same preprocessing. Three unsupervised CycleGAN-based models were respectively trained with different sequence MRI with Architecture, which consists of 2 ResUnet-based generators and 2 Patch-GAN-based discriminators. The remaining 25 CT-MR cases with non-rigid registration were selected as the independent paired testing set. The mean error (ME) and mean absolute error (MAE) between plan CT and sCT of each model were used to measure the CT value estimation accuracy, which was calculated within air (Hounsfield Unit, HU < 100), soft tissue (-100 < HU < 150), bone (HU > 150) and whole body, respectively. Structural similarity index (SSIM) was applied to evaluate the similarity between error (plan CT and sCT. RESULTS: The MEs calculated between plan CT and T1, T2, T1C-based sCT images, were (-0.62 ± 3.06) vs. (-1.81 ± 3.13) vs. (0.13 ± 2.56) within air, (154.92 ± 87.93) vs. (173.27 ± 97.62) vs. (262.78 ± 91.74) within soft tissue, (1363.99 ± 294.94) vs. (337.21 ± 50.4) vs. (661.63 ± 168.43) within bone, (22.12 ± 29.47) vs. (17.05 ± 10.51) vs. (35.28 ± 20.75) within the whole body. The MAEs correspondingly were (4.49 ± 3.13) vs. (4.6 ± 3.47) vs. (4.6 ± 3.47) within air, (186.41 ± 61.75) vs. (197.33 ± 58.62) vs. (293.56 ± 72.72) within soft tissue, (186.41 ± 61.75) vs. (197.33 ± 58.62) vs. (293.56 ± 72.72) within bone, (31.28 ± 28.1) vs. (27.64 ± 10.41) vs. (42.6 ± 23.82) within the whole body. The quantitative analysis between T1, T2, T1C-sCTs indicates that the T1C based generated model achieves higher accuracy than the other models in nasopharyngeal carcinoma. CONCLUSION: The T1C MRI can provide sufficient anatomy information and tissue contract in synthetic CT prediction of head and neck region, which offers a substantial potential for density estimation and dose calculation in radiotherapy. The generation method and evaluation will investigate synthetic CT prediction with more complex tissue in various body parts.

16.
Int J Radiat Oncol Biol Phys ; 111(3S): e86-e87, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701986

RESUMO

PURPOSE/OBJECTIVE(S): Host immune status plays an important role in treatment outcome prediction and prognosis in patients with various cancers. However, the relationship between circulating immune cell and tumor control has not been well studied in hepatocellular carcinoma (HCC) after stereotactic body radiation therapy (SBRT). MATERIALS/METHODS: Patients with recurrent HCC received liver SBRT were eligible. Patients must be deemed surgically unresectable, and had Trans Arterial Chemotherapy Embolization (TACE). The local tumor control was defined as no disease progression by treatment response assessment CT or MRI around 3 months, largely according to mRECIST. Complete metabolic responses were defined either with no enhancement on MRI or no FDG activity. The risk factors of our interest included demographics factors, liver disease history, Child Pugh score, pathology stage, and prior treatments. The counts of whole leukocytes, lymphocyte and neutrophil pre- and post-SBRT were recorded from clinical laboratory. Leukopenia was graded according to CTCAE v 5.0. The associations of variables with in-field treatment response were analyzed using Chi-square test and one-way ANOVA. RESULTS: A total of 22 patients enrolled between August 2015 and September 2020. All patients had disease progression after TACE treatment and received 5 fractions of SBRT. Twenty patients received a definitive dose of 40-50 Gy. The local tumor was controlled in 86.4% (19/22), and increased to 90% (18/20) after excluding 2 patients receiving lower doses of palliative SBRT. There were 2 (10%), 5 (25%) and 13 (65%) defined as progressive disease, stable disease, partial response, and complete metabolic response, respectively. Large tumor/multiple tumor (P = 0.003), vascular invasion (P = 0.042), surgery history (P = 0.023), and BCLC stage (P = 0.040) were significantly associated with the tumor control, while age, gender, family history, heavy drinking, smoking history, BMI, ECOG, hepatitis, cirrhosis, Child Pugh score, portal vein tumor emboli, TNM, tumor size, and radiation dose were not. In 20 patients with complete blood testing peri-SBRT and uninterrupted radiation treatment, 17 (85%), 18 (90%), 13 (65%) and 9 (45%) patients had reduction in leukocyte, lymphocyte, neutrophil and monocyte after SBRT, respectively. Post-SBRT leukocyte counts (P = 0.036), pre-monocyte (P = 0.029), pre-lymphocyte (P = 0.073) and post- lymphocyte (P = 0.092) were associated with tumor control. The pre-RT leukocyte, neutrophil, post-RT neutrophil and monocyte were not. CONCLUSION: This limited study suggests that SBRT may provide excellent local tumor control for patients with recurrent HCC after surgical resection and TACE with no local treatment options. The significance in post-SBRT circulating immune cell suggest a role of immune function on radiation tumor control and a potential value of improving SBRT technique to decrease damage of immune cells. Further validation study with large sample size and longer follow-up is needed.

17.
J Phys Condens Matter ; 33(49)2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34521073

RESUMO

Lattice thermal conductivity (LTC) is a key parameter for many technological applications. Based on the Peierls-Boltzmann transport equation (PBTE), many unique phonon transport properties of various materials were revealed. Accurate calculation of LTC with PBTE, however, is a time-consuming task, especially for compounds with a complex crystal structure or taking high-order phonon scattering into consideration. Graphical processing units (GPUs) have been extensively used to accelerate scientific simulations, making it possible to use a single desktop workstation for calculations that used to require supercomputers. Due to its fundamental differences from traditional processors, GPUs are especially suited for executing a large group of similar tasks with minimal communication, but require completely different algorithm design. In this paper, we provide a new algorithm optimized for GPUs, where a two-kernel method is used to avoid divergent branching. A new open-source code, GPU_PBTE, is developed based on the proposed algorithm. As demonstrations, we investigate the thermal transport properties of silicon and silicon carbide, and find that accurate and reliable LTC can be obtained by our software. GPU_PBTE performed on NVIDIA Tesla V100 can extensively improve double precision performance, making it two to three orders of magnitude faster than our CPU version performed on Intel Xeon CPU Gold 6248 @2.5 GHz. Our work also provides an idea of accelerating calculations with other novel hardware that may come out in the future.

18.
Phys Rev E ; 104(2-2): 025107, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34525656

RESUMO

The variation of the distribution of the liquid flow in porous media during evaporation is still a puzzle. We resolve it with the pore network modeling approach. The distribution of the evaporation-induced liquid flow in a pore network composed of about 2.5 million pores is determined. The probability density function of the magnitude of the normalized liquid flow rate is obtained. For the low normalized liquid flow rate, the probability density function is power-lawlike. The power-law exponent depends on both the liquid saturation and the location of the moving meniscus in the main liquid cluster. The evaporation-induced liquid flow in the pores in the pore network can be correlated. Whether the liquid flow distributions in various zones in the pore network are similar or not relies significantly on the location of the moving meniscus in the main liquid cluster. The functions depicting the relation between the power-law exponent and the local liquid saturation for the zones adjacent to and away from the open side of the pore network are different. These findings from the pore scale studies provide insights into developing the accurate continuum model for evaporation in porous media.

19.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 33(4): 401-405, 2021 Aug 24.
Artigo em Chinês | MEDLINE | ID: mdl-34505448

RESUMO

OBJECTIVE: To investigate the prevalence and changing trend of Enterobius vermicularis infections among children in Shandong Province, so as to provide the scientific evidence for the adjustment and development of the enterobiasis control strategy. METHODS: Soil-borne nematodiasis surveillance sites were assigned in 51 counties (districts, cities) in Shandong Province from 2016 to 2020, and the E. vermicularis infections were detected using a modified Kato-Katz technique and the cellophane tape method among children at ages of 3 to 9 years living in these surveillance sites. The epidemiological profiles of E. vermicularis-infected children were descriptively analyzed. RESULTS: A total of 5 060 children at ages of 3 to 9 years were detected in 51 soil-borne nematodiasis surveillance sites in Shandong Province from 2016 to 2020, and the overall prevalence of E. vermicularis infections was 2.23%. The annual prevalence of E. vermicularis infections was 3.99% (26/651), 1.70% (14/824), 0.96% (8/837), 2.90% (45/1 552) and 1.67% (20/1 196) from 2016 to 2020, respectively, with a significant difference detected among years (χ2 = 21.455, P < 0.01). The prevalence of E. vermicularis infections was 1.25% (15/1 198), 1.85% (14/755), 3.18% (84/2 640) and 0 (0/467) among children from central, eastern, southern and northern Shandong Province (χ2 = 27.326, P < 0.01). In addition, there was no significant difference in the prevalence of E. vermicularis infections between male (1.98%, 56/2 831) and female children (2.56%, 57/2 229) (χ2 = 1.916, P > 0.05); however, there was age-specific prevalence of E. vermicularis infections among children (χ2 = 16.448, P < 0.05), with the greatest prevalence detected among children at ages of 6 years (3.18%, 25/786), and the lowest prevalence seen among children at ages of 3 years (0.75%, 6/800). CONCLUSIONS: The prevalence of E. vermicularis infections remained at a medium level among children at ages of 3 to 9 years in Shandong Province from 2016 to 2020, with region-specific prevalence found across the province. An integrated strategy is required for enterobiasis control.


Assuntos
Enterobíase , Infecções por Nematoides , Animais , Criança , Pré-Escolar , Cidades , Enterobíase/epidemiologia , Enterobius , Feminino , Humanos , Masculino , Prevalência , Solo
20.
Anaesth Rep ; 9(2): e12128, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34396134

RESUMO

Epidural catheters are often used in the provision of surgical anaesthesia and analgesia. These catheters need to be removed after a certain period, but difficulties may be encountered during this process. We present a case of difficult epidural catheter removal in an elderly woman who had undergone surgery for reduction and internal fixation of a comminuted intertrochanteric fracture at the left femur under combined spinal epidural anaesthesia. Anaesthesia was satisfactory during the operation; however, initial attempts to remove the catheter at the end of the procedure was met with significant resistance. Normal saline (5 ml) was injected through the catheter without difficulty, suggesting that the catheter was likely trapped and compressed but not obstructed. A nickel/titanium memory alloy suture was used as a guidewire to remove the catheter without damaging it. This case was analysed subsequently, and the management of future problematic removals is discussed herein. This article aims to raise awareness of, and to explore techniques for managing difficult epidural catheter removal.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...