Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 37
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Food Sci ; 2020 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-32449946

RESUMO

In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D). The results of the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the formation of WPI-D. Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the ß-sheet content was decreased and the tryptophan content was increased. The emulsifying properties and the ability to encapsulate ß-carotene of WPI-D were improved compared with WPI (P < 0.05). When the reaction temperature was 70 and 80 °C, the EG and the ability to encapsulate ß-carotene of WPI-D were better (P < 0.05), which was related to protein unfolding. However, due to the polymerization between the WPI molecules, the emulsion activity index of WPI-D and the ability to encapsulate ß-carotene were lowered at 90 °C (P < 0.05). Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate ß-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation. PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate ß-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.

2.
PLoS One ; 15(3): e0230200, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32214324

RESUMO

Statins, a class of drugs that can effectively remove cholesterol from serum, are used to regulate plasma total cholesterol and reduce the risk of cardiovascular diseases, but it is still unclear whether the drug are modulated by gut microbiota or the structures of gut microbiota are shaped by statins. We investigated the interactions between statins and the human gut microbiota during the in vitro fermentation process by 16S rRNA gene sequencing, gas chromatography (GC), and high-performance liquid chromatography (HPLC) analyses. The presence of fluvastatin (FLU2) specifically promoted the growth of Escherichia/Shigella, Ruminococcaceae UCG 014, and Sutterella. However, the composition of the gut bacterial microbiota remained relatively static in samples treated with rosuvastatin (ROS), simvastatin (SIM), and atorvastatin (ATO). The PICRUSt program predicted moderate differences in the functional categories related to the biosynthesis of other secondary metabolites, cellular processes and signaling, and signal transduction in the FLU2 fermentation samples. Our study revealed substantial variation in the structure and function of microbiomes from the FLU2-treated samples. In addition, short-chain fatty acids (SCFAs) were also significantly decreased in FLU2-treated samples compared with the samples treated with other stains. Statins can be degraded by the human gut microbiota in vitro, and the degradation rate was approximately 7%-30% and 19%-48% after fermentation was allowed to proceed for 24 h and 48 h, respectively. Generally, FLU2 could largely shape the composition and function of human gut microbiota, which resulted in changes in the production of SCFAs. In turn, all statins could be degraded or modified by the gut microbiota. Our study paves the way for elucidating statin-gut microbiota interactions in vitro towards the improvement of the host health and personalized medicine.

3.
J Vis Exp ; (150)2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31498314

RESUMO

Human intestinal microorganisms have recently become an important target of research in promoting human health and preventing diseases. Consequently, investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota have become an important research topic. However, in vivo experiments with human volunteers are not ideal for such studies due to bioethics and economic constraints. As a result, animal models have been used to evaluate these interactions in vivo. Nevertheless, animal model studies are still limited by bioethics considerations, in addition to differing compositions and diversities of microbiota in animals vs. humans. An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro. To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic. Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs). Presented here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems. Importantly, this protocol can also be modified to investigate general interactions between other endobiotics and gut microbiota.

4.
ACS Appl Mater Interfaces ; 11(36): 33124-33131, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31424185

RESUMO

Highly porous GaN submicron rods have been synthesized successfully by a facile hydrothermal method and heat treatment under controlled atmosphere. The morphology and size of the hydrothermal products are tailorable by adjusting the concentration of precursor solutions. Upon calcination in air, the nanorod-assembled GaOOH submicron rods are converted into bundlelike Ga2O3 and into porous GaN submicron rods under an ammonia flow. Gas-sensing characterization demonstrates that the sensors based on porous GaN exhibit high sensitivity and fast response to ethanol vapor, as well as excellent stability and reliability at high temperature. The highly porous GaN submicron rods with a large specific surface area, small grain size, and high length-to-diameter ratio show better response to ethanol. A possible sensing enhancement mechanism is also proposed. This study provides a promising route for the novel synthesis of GaN submicron rods for high-performance gas sensors.

5.
Food Sci Nutr ; 7(8): 2636-2646, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31428351

RESUMO

The nonalcoholic fatty liver disease (NAFLD) is a progressive liver disease that affects the health of people in an increasing rate. In the current research, we investigated the beneficial effect of a novel probiotic strain L. paracasei Jlus66 (Jlus66) on rats with high-fat diet (HFD)-induced NAFLD. The intestinal flora of rats was analyzed based on V3-V4 region 16S rDNA sequencing. Moreover, we measured the oxidative stress and inflammation factors in the liver using commercial ELISA kit, and the lipopolysaccharide (LPS) in serum with chromogenic end-point tachypheus amebocyte lysate. Compared with the HFD-induced group, Jlus66 treatment significantly decreased the malondialdehyde (MDA) level in the serum (p < 0.05). Additionally, Jlus66 significantly enhanced the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver and serum (p < 0.05). Jlus66 administration also reduced the levels of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6), and inversely increased the interleukin-10 (IL-10) level in serum (p < 0.05). Intestinal flora analysis results showed that Jlus66 can improve intestinal flora structure by increasing the abundance of gram-positive flora such as Firmicutes, and decreasing gram-negative flora such as Bacteroidetes, Proteobacteria, and Fusobacteria, and then reduced LPS concentration in the serum. So we concluded that Jlus66 can improve NAFLD by modulating the intestinal flora and followed reduction of oxidative stress (OxS) and inflammation.

6.
GM Crops Food ; 10(3): 181-190, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31366287

RESUMO

Glyphosate tolerant soybeans represent a large portion of soybeans grown and fed to farm animals around the world. Despite their widespread use for many years, some have raised questions regarding their safety because the soybeans were genetically modified. The CP4 EPSPS gene which imparts resistance to topical application of the herbicide glyphosate was introduced into soybeans. Application of glyphosate to soybean fields will reduce weed pressure and increase soybean yield. To assess their safety on the rat reproduction system, male Sprague Dawley rats were fed either glyphosate-tolerant (GM) soybean (40-3-2) or near-isogenic, non-GM (A5403) (control) soybean meal. The processed soybean meal was added to formulated rodent diets at 20% (w/w) and fed to rats for 90 days. Some rats from the control group were separately administered mitomycin C for 40 days and served as positive controls in the sperm abnormality test. Body weights and behavior were monitored daily, serum enzymes and histologic and EM appearance of the testis, and sperm morphology were also examined. After 90 days of feeding, no adverse effects were observed in rats fed glyphosate-tolerant soybeans.


Assuntos
Glicina/análogos & derivados , Herbicidas/efeitos adversos , Soja/genética , Testículo/efeitos dos fármacos , Ração Animal , Animais , Comportamento Animal/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Glicina/efeitos adversos , Resistência a Herbicidas , Masculino , Mitomicina/administração & dosagem , Mitomicina/farmacologia , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Ratos , Ratos Sprague-Dawley , Soja/crescimento & desenvolvimento
7.
J Zhejiang Univ Sci B ; 20(7): 613-616, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31168975

RESUMO

The ovum oil of forest frog has various health beneficial functions. In the current research, we evaluated the hypolipidemic effects of the low-cholesterol ovum oil from the forest frog and its combination with stigmasterol in rats.


Assuntos
Colesterol/metabolismo , Hiperlipidemias/tratamento farmacológico , Óleos/farmacologia , Ranidae , Estigmasterol/farmacologia , Animais , Ácidos Graxos Insaturados/farmacologia , Feminino , Lipídeos/sangue , Masculino , Óvulo/química , Ratos , Ratos Sprague-Dawley
8.
Microsyst Nanoeng ; 5: 21, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31123595

RESUMO

In a high relative humidity (RH) environment, it is challenging for ethanol sensors to maintain a high response and excellent selectivity. Herein, tetragonal rutile SnO2 nanosheets decorated with NiO nanoparticles were synthesized by a two-step hydrothermal process. The NiO-decorated SnO2 nanosheet-based sensors displayed a significantly improved sensitivity and excellent selectivity to ethanol gas. For example, the 3 mol% NiO-decorated SnO2 (SnO2-3Ni) sensor reached its highest response (153 at 100 ppm) at an operating temperature of 260 °C. Moreover, the SnO2-3Ni sensor had substantially improved moisture resistance. The excellent properties of the sensors can be attributed to the uniform dispersion of the NiO nanoparticles on the surface of the SnO2 nanosheets and the formation of NiO-SnO2 p-n heterojunctions. Considering the long-term stability and reproducibility of these sensors, our study suggests that the NiO nanoparticle-decorated SnO2 nanosheets are a promising material for highly efficient detection of ethanol.

9.
Food Sci Nutr ; 7(4): 1295-1301, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31024702

RESUMO

Obesity has become one of the most important health problems worldwide requiring urgent need for efficient control. Pleurotus citrinopileatus (P. citrinopileatus)-a type of edible mushroom with abundant bioactive molecules-is a promising source for achieving this goal. In the present study, we evaluated the anti-obesity and hypolipidemic effect of P. citrinopileatus water extract (PWE) using a series of biochemical assays in randomized high-fat diet-induced obese (DIO) C57BL/6J mice, which were gavaged daily with low or high levels of PWE (400 or 800 mg/kg of body weight, respectively) in addition to high-fat diet for 12 weeks. Results showed that PWE significantly reduced the weight gain, fat accumulation, and food intake of DIO mice within 12 weeks. PWE also decreased the serum triglycerides, cholesterol and low-density lipoprotein, aspartate transaminase, nonesterified fatty acid, and creatinine, but increased high-density lipoprotein. Additionally, PWE improved the glucose tolerance of mice fed with high fat. From above, we conclude that PWE has great potential as functional foods for management of obesity and/or associated metabolic disorders.

10.
Food Nutr Res ; 622018.
Artigo em Inglês | MEDLINE | ID: mdl-30574053

RESUMO

Background: Previous studies suggested that probiotics intervention may be one of the methods for preventing and/or treating gastric ulcer. Objective: The aim of the study was to compare the preventive effects of a spaceflight mutant Lactobacillus reuteri F-9-35 and its wild type on ethanol-induced gastric injury in rats. Design: Forty rats were randomly allocated into five groups: a normal group (NOR), ethanol group (EtOH), skim milk group (MILK), L. reuteri F-9-35 group (F935) and wild-type group (WT). The NOR and EtOH groups received 1 ml of distilled water by daily gavage for 14 days. The MILK group received 1 ml of skim milk alone, while the F935 and WT groups were administered 1 ml of skim milk containing the mutant and wild type (1 × 1010 colony-forming unit/ml) by daily gavage for 14 days, respectively. Acute gastric injury was induced by absolute alcohol 1 h after the final administration of different treatments, except for the NOR group. Results: Pretreatment with L. reuteri F-9-35, but not milk alone or milk with the L. reuteri wild type, showed significant reduction of ethanol-induced gastric injury, as evidenced by lowering of ulcer index, ulcer area (%), and histological lesion. F-9-35 decreased the levels of lipid peroxidation and myeloperoxidase and increased mucus, glutathione, and nitric oxide levels in gastric tissue. Moreover, F-9-35 inhibited the expression of proinflammatory genes including gastric tumor necrosis factor-α, interleukin-1ß, and cyclooxygenase-2 and decreased the activity of nuclear factor kappa B (NF-κB). Conclusion: These findings indicated that L. reuteri F-9-35 pretreatment can attenuate ethanol-induced gastric injury in rats by inhibiting oxidative stress and inflammatory response. Together, L. reuteri F-9-35 has potential preventive efficacy on gastric ulcer.

11.
Food Sci Nutr ; 6(8): 2537-2544, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30510755

RESUMO

Beta-carotene, the main dietary source of provitamin A, is required for maintaining optimum human health. The bioaccessibility of beta-carotene can be greatly improved when ingested with fat. Therefore, the aim of the current study was to select proper oils (palm oil, coconut oil, fish oil, and corn oil) as a carrier to form stable nanoemulsion that can effectively enhance the bioaccessibility of beta-carotene. The nanoemulsion was formulated with 90% (v/v) aqueous solution (2% whey protein isolate, WPI, w/v) and 10% (v/v) dispersed oil. The in vitro digestion experiment of nanoemulsions showed that the bioaccessibility of beta-carotene was as followed in order: palm oil = corn oil > fish oil > coconut oil (p < 0.05). The particle size of the nanoemulsion (initial particle size = 168-185 nm) was below 200 nm during 42 days' storage at 25°C. The retention rates of beta-carotene in nanoemulsions were 69.36%, 63.81%, 49.58%, and 54.91% with palm oil, coconut oil, fish oil, and corn oil, respectively. However, the particle size of the nanoemulsion increased significantly in the accelerated experiment at 55°C (p < 0.05), in which the retention rates of beta-carotene were 48.56%, 43.41%, 29.35%, and 33.60% with palm oil, coconut oil, fish oil, and corn oil, respectively. From above, we conclude that WPI-stabilized beta-carotene nanoemulsion with palm oil as the carrier is the most suitable system to increase bioaccessibility and stability of lipid-soluble bioactive compounds such as beta-carotene.

12.
Molecules ; 23(12)2018 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-30513668

RESUMO

Hericium erinaceus (HE) is a well-known edible and medicinal fungus widely grown in Asian countries. Polysaccharides from the Hericium erinaceus (HEP) are major biological macromolecules. It has been reported that HEP has multiple biological activities, such as antioxidant activity, immunomodulatory effects, anti-inflammatory effect, anti-chronic gastritis activity, and so on. In the current study, we investigated the biological property of HEP during gastrointestinal digestion. The results indicated that both simulated gastric and small intestinal digesta of HEP has better stimulation of probiotics growth than HEP alone, especially for Lactobacillus plantarum BG112. The prebiotic activity was the strongest when HEP was treated by simulated gastric juice for 2 h and by simulated small intestinal juice for 4 h. The molecular weight (Mw) of HEP decreased from 1.68 × 106 Da and 2.32 × 104 Da to 529.3 ± 7.2 Da, as digestion time increased. Meanwhile, the reducing sugar content was significantly increased from 0.610 ± 0.007 to 22.698 ± 0.752 mg/ml, suggesting that the decrease of Mw was likely due to the breakdown of glycosidic bonds. Considerable mannose and galactopyranose were released throughout the gastrointestinal digestion period, indicating that the gastrointestinal digestion resulted in production of free monosaccharides. After fermentation of L. plantarum BG112, the Mw of HEP was decreased and short chain fatty acids (SCFAs) including acetic acid, isovaleric acid, lactic acid, and butyric acid were produced. We speculated that the release of free monosaccharides during gastrointestinal digestion and utilization of HEP, by the probiotics, contributed to the prebiotic activity of HEP's gastric and intestinal digesta. These results unveiled some mechanisms on the close relationship between the structure and bioactivity of polysaccharides, during digestion.


Assuntos
Basidiomycota/química , Intestinos/química , Polissacarídeos/farmacologia , Prebióticos , Estômago/química , Digestão , Ácidos Graxos/biossíntese , Fermentação , Cromatografia Gasosa-Espectrometria de Massas , Suco Gástrico/efeitos dos fármacos , Peso Molecular , Monossacarídeos/análise , Padrões de Referência
13.
Food Sci Nutr ; 6(7): 1940-1949, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30349684

RESUMO

Thermal treatment has been utilized to improve the functional properties of proteins for many years. In this study, we aimed to investigate the effect of sodium triphosphate (Na5P3O10) on particle size and size distribution of heat-induced whey protein concentrate (WPC) aggregates under different processing conditions. The results showed that high Na5P3O10 level (>0.5%, w/w), long heating time (>15 min), and alkaline condition (pH 8-8.5) facilitated formation of large particles (>10 µm). The WPC aggregates with small-to-medium particle size (1-3 µm) that are suitable to be applied as a fat replacer were obtained by heating the WPC solution (8%, w/v) containing 0.4% (w/w) Na5P3O10 at 85°C for 5 min. We conclude that thermal treatment of whey protein concentrate added with Na5P3O10 can obtain whey protein products with different particle sizes for certain applications.

14.
J Food Sci ; 83(10): 2645-2652, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30216448

RESUMO

Probiotics are considered to be a potential treatment for ulcerative colitis (UC). The aim of this study was to compare the preventive effect of a space flight-induced mutant L. reuteri F-9-35 and its wild type on UC in vivo. Female mice were randomly assigned to five groups: one normal and four colitic. Mice from colitis groups were daily gavaged with 0.2 mL 12% (w/v) skim milk containing the mutant or wild type (1 × 1011 CFU/mL), skim milk alone or distilled water for the whole experiment period, starting 7 days before colitis induction. UC was induced by administrating mice with 3.5% (w/v) dextran sulfate sodium (DSS) in drinking water for 7 days, after which DSS was removed and maintained for 3 days as a recovery phase. The results showed that the mice fed with L. reuteri F-9-35 had less inflammatory phenotype according to macroscopic and histological analysis, reduced myeloperoxidase activity, and lower expression of proinflammatory genes (Tumor necrosis factor-α, cyclooxygenase-2 and interleukin-6) in colonic tissue compared with control. Furthermore, L. reuteri F-9-35 protected the mice from gut microbiota dysbiosis from DDS induced colitis. Neither wild type nor the milk alone had such beneficial effects. From above we conclude that L. reuteri F-9-35 has great potential in the prevention of UC as a dietary supplement. PRACTICAL APPLICATION: Ulcerative colitis (UC) is the most common inflammatory bowel diseases and there is still a lack of safe and effective treatments. Consumption of L. reuteri F-9-35 may effective in preventing human UC.


Assuntos
Colite/prevenção & controle , Colo/microbiologia , Microbioma Gastrointestinal , Lactobacillus reuteri/fisiologia , Probióticos/uso terapêutico , Animais , Colite/induzido quimicamente , Colo/metabolismo , Sulfato de Dextrana , Modelos Animais de Doenças , Feminino , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Inflamação , Doenças Inflamatórias Intestinais/metabolismo , Interleucina-6/metabolismo , Camundongos , Peroxidase/metabolismo , Fenótipo , Fator de Necrose Tumoral alfa/metabolismo
15.
Nutrients ; 10(8)2018 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-30096921

RESUMO

With the rapid development of modern society, many chronic diseases are increasing including diabetes, obesity, cardiovascular diseases, etc., which further cause an increased death rate worldwide. A high caloric diet with reduced natural polysaccharides, typically indigestible polysaccharides, is considered a health risk factor. With solid evidence accumulating that indigestible polysaccharides can effectively prevent and/or ameliorate symptoms of many chronic diseases, we give a narrative review of many natural polysaccharides extracted from various food resources which mainly contribute their health beneficial functions via intestinal fermentation.


Assuntos
Bactérias/metabolismo , Fibras na Dieta/metabolismo , Digestão , Fermentação , Microbioma Gastrointestinal , Intestinos/microbiologia , Polissacarídeos/metabolismo , Animais , Humanos
16.
Food Sci Nutr ; 6(4): 1053-1064, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29983970

RESUMO

Effect of ultrasound treatment on the physicochemical properties and structure of ß-lactoglobulin were investigated. ß-Lactoglobulin was treated with ultrasound at different amplitudes, temperatures, and durations. The surface hydrophobicity and free sulfhydryl group of ß-lactoglobulin were significantly increased after ultrasound treatment (p < .05). The maximal surface hydrophobicity and free sulfhydryl group were 5,812.08 and 5.97 µmol/g, respectively. Ultrasound treatment changed the physicochemical properties of ß-lactoglobulin including particle size (from 1.21 ± 0.05 nm to 1.66 ± 0.03 nm), absolute zeta potential (from 15.47 ± 1.60 mV to 27.63 ± 3.30 mV), and solubility (from 84.66% to 95.17%). Ultrasound treatment increased α-helix and ß-sheet structures of ß-lactoglobulin. Intrinsic fluorescence intensity of ultrasound-treated ß-lactoglobulin was increased with shift of λmax from 334 to 329 nm. UV absorption of ß-lactoglobulin was decreased with shift of λmax from 288 to 285 nm after ultrasound treatment. There were no significant changes in high-performance liquid chromatography and protein electrophoretic patterns. These findings indicated that ultrasound treatment had high potential in modifying the physiochemical and structural properties of ß-lactoglobulin for industrial applications.

17.
Sci Rep ; 8(1): 3527, 2018 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-29476144

RESUMO

Alliin (S-allyl cysteine sulfoxide) is a bioactive sulfoxide compound derived from garlic. To evaluate the preventive effect of alliin against metabolic risk factors in diet induced obese (DIO) mice, we treated the C57BL/6J DIO mice with drinking water with or without alliin (0.1 mg/ml) for 8 weeks. Results showed that alliin had no significant effect on the body weight, adiposity or energy balance. However, alliin treatment enhanced glucose homeostasis, increased insulin sensitivity and improved the lipid profile in the DIO mice. This was, at least partly, attributable to alliin induced modulation of the intestinal microbiota composition, typically decreased Lachnospiraceae and increased Ruminococcaceae. From above, we conclude that alliin has nutraceutical or even medicinal potential in prevention of diabetes and lipid metabolic disorders.


Assuntos
Cisteína/análogos & derivados , Glucose/metabolismo , Hiperlipidemias/tratamento farmacológico , Hipoglicemiantes/farmacologia , Hipolipemiantes/farmacologia , Obesidade/tratamento farmacológico , Administração Oral , Animais , Composição Corporal , Peso Corporal , Clostridiales/classificação , Clostridiales/efeitos dos fármacos , Clostridiales/fisiologia , Cisteína/farmacologia , Dieta Hiperlipídica/efeitos adversos , Água Potável/administração & dosagem , Metabolismo Energético/efeitos dos fármacos , Metabolismo Energético/fisiologia , Microbioma Gastrointestinal/efeitos dos fármacos , Microbioma Gastrointestinal/fisiologia , Teste de Tolerância a Glucose , Homeostase/fisiologia , Hiperlipidemias/etiologia , Hiperlipidemias/metabolismo , Hiperlipidemias/fisiopatologia , Insulina/metabolismo , Resistência à Insulina/fisiologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/fisiopatologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/etiologia , Obesidade/metabolismo , Obesidade/fisiopatologia , Gordura Subcutânea/efeitos dos fármacos , Gordura Subcutânea/metabolismo , Gordura Subcutânea/fisiopatologia
18.
PLoS One ; 13(2): e0192511, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29415071

RESUMO

Lutein is a hydrophobic carotenoid that has multiple health functions. However, the application of lutein is limited due to its poor solubility in water and instability under certain conditions during storage. Hereby we generated lutein loaded nano-emulsions using whey protein isolate (WPI) or polymerized whey protein isolate (PWP) with assistance of high intensity ultrasound and evaluate their stability during storage at different conditions. We measured the particle size, zeta-potential, physical stability and lutein content change. Results showed that the PWP based nano-emulsion system was not stable in the tested Oil/Water/Ethanol system indicated by the appearance of stratification within only one week. The WPI based nano-emulsion system showed stable physiochemical stability during the storage at 4°C. The lutein content of the system was reduced by only 4% after four weeks storage at 4°C. In conclusion, our whey protein based nano-emulsion system provides a promising strategy for encapsulation of lutein or other hydrophobic bioactive molecules to expand their applications.


Assuntos
Emulsões , Luteína/química , Nanotecnologia , Proteínas do Soro do Leite/química , Tamanho da Partícula , Polimerização
19.
J Agric Food Chem ; 66(6): 1472-1478, 2018 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-29345928

RESUMO

Astaxanthin nanodispersion was prepared using whey protein isolate (WPI) and polymerized whey protein (PWP) through an emulsification-evaporation technique. The physicochemical properties of the astaxanthin nanodispersion were evaluated, and the transport of astaxanthin was assessed using a Caco-2 cell monolayer model. The astaxanthin nanodispersions stabilized by WPI and PWP (2.5%, w/w) had a small particle size (121 ± 4.9 and 80.4 ± 5.9 nm, respectively), negative ζ potential (-19.3 ± 1.5 and -35.0 ± 2.2 mV, respectively), and high encapsulation efficiency (92.1 ± 2.9 and 93.5 ± 2.4%, respectively). Differential scanning calorimetry curves indicated that amorphous astaxanthin existed in both astaxanthin nanodispersions. Whey-protein-stabilized astaxanthin nanodispersion showed resistance to pepsin digestion but readily released astaxanthin after trypsin digestion. The nanodispersions showed no cytotoxicity to Caco-2 cells at a protein concentration below 10 mg/mL. WPI- and PWP-stabilized nanodispersions improved the apparent permeability coefficient (Papp) of Caco-2 cells to astaxanthin by 10.3- and 16.1-fold, respectively. The results indicated that whey-protein-stabilized nanodispersion is a good vehicle to deliver lipophilic bioactive compounds, such as astaxanthin, and to improve their bioavailability.


Assuntos
Proteínas do Soro do Leite/química , Disponibilidade Biológica , Transporte Biológico , Células CACO-2 , Varredura Diferencial de Calorimetria , Estabilidade de Medicamentos , Emulsões/química , Emulsões/metabolismo , Excipientes/química , Humanos , Tamanho da Partícula , Xantofilas/química , Xantofilas/metabolismo
20.
Food Funct ; 8(12): 4539-4546, 2017 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-29106426

RESUMO

Nonalcoholic fatty liver disease (NAFLD) is the main cause of chronic liver disease worldwide. Previous evidence indicates that probiotics can be applied as a therapeutic agent for NAFLD. In this study, the potential probiotic strain Lactobacillus paracasei Jlus66 was isolated from natural fermented milk by a culture-dependent method, and its probiotic potentials were tested by established in vitro tests. In addition, the protective effect of Lactobacillus paracasei Jlus66 against NAFLD was evaluated in rat models. Compared with the high-fat-diet (HFD) group, the rats administered with 4 × 1010 cfu Jlus66 had significantly lower body weight gain, serum triglyceride (TG), low-density lipoprotein (LDL) as well as aminotransferase (ALT). Histopathological analysis showed Jlus66 also reduced the level of hepatic triglycerides and steatosis. From the above we conclude that L. paracasei Jlus66 has great potential as a probiotic in protecting from NAFLD.


Assuntos
Lactobacillus paracasei/isolamento & purificação , Leite/microbiologia , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Probióticos/administração & dosagem , Animais , Bovinos , Dieta Hiperlipídica , Fermentação , Humanos , Lactobacillus paracasei/classificação , Lactobacillus paracasei/genética , Lactobacillus paracasei/metabolismo , Lipoproteínas LDL/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Leite/química , Hepatopatia Gordurosa não Alcoólica/metabolismo , Probióticos/isolamento & purificação , Probióticos/metabolismo , Ratos , Ratos Wistar , Triglicerídeos/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA