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1.
Sci Adv ; 7(4)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33523953

RESUMO

Wearable sensing technology is an essential link to future personalized medicine. However, to obtain a complete picture of human health, it is necessary but challenging to track multiple analytes inside the body simultaneously. Here, we present a wearable plasmonic-electronic sensor with "universal" molecular recognition ability. Flexible plasmonic metasurface with surface-enhanced Raman scattering (SERS)-activity is introduced as the fundamental sensing component in a wearable sensor since we solved the technical challenge of maintaining the plasmonic activities of their brittle nanostructures under various deformations. Together with a flexible electronic sweat extraction system, our sensor can noninvasively extract and "fingerprint" analytes inside the body based on their unique SERS spectra. As a proof-of-concept example, we successfully monitored the variation of trace-amounts drugs inside the body and obtained an individual's drug metabolic profile. Our sensor bridges the existing gap in wearable sensing technology by providing a universal, sensitive molecular tracking means to assess human health.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33528246

RESUMO

It is challenging to hydrothermally synthesize solution-processable MoS2, as the strong van der Waals force between MoS2 nanosheets induces self-assembly of agglomerates. Here, we introduce poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) into the precursor to impede aggregate formation in the hydrothermal process. A hybrid MoS2/PEDOT:PSS (MP) hydrogel is formed due to the electrostatic interactions between the negatively charged MoS2 and positively charged PEDOT chains. This hydrogel can be easily dispersed in water for subsequent solution processing such as vacuum filtration to form free-standing flexible films or extrusion 3D printing to create novel patterns. The MP film with a fracture strength of 18.59 MPa displays excellent electrochemical performance in both aqueous Na2SO4 electrolyte (474 mF cm-2) and solid-state PVA-H3PO4 electrolyte (360 mF cm-2). Flexibility and robustness can be evidenced by high capacitance retention rates of 94 and 89% after being repeatedly bent to 180° for 5000 cycles in aqueous and solid-state electrolytes, respectively.

3.
EuroIntervention ; 2021 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-33528359

RESUMO

BACKGROUND: Intravascular optical coherence tomography (IVOCT) enables detailed plaque characterisation in-vivo, but visual assessment is time-consuming and subjective. AIMS: This study aims to develop and validate an automatic framework for IVOCT plaque characterisation using artificial intelligence (AI). METHODS: IVOCT pullbacks from 5 international centres were analysed in a corelab, annotating basic plaque components, inflammatory markers and other structures. A deep convolutional network with encoding-decoding architecture and pseudo-3D input was developed and trained using hybrid loss. The proposed network was integrated into commercial software to be externally validated on additional IVOCT pullbacks from three international corelabs, taking the consensus among corelabs as reference. RESULTS: Annotated images from 509 pullbacks (391 patients) were divided into 10,517 and 1,156 cross-sections for the training and testing datasets, respectively. Dice coefficient of the model was 0.906 for fibrous plaque, 0.848 for calcium and 0.772 for lipid in the testing dataset. Excellent agreement in plaque burden quantification was observed between the model and manual measurements (R2=0.98). In the external validation, the software correctly identified 518 out of 598 plaque regions from 300 IVOCT cross-sections, with a diagnostic accuracy of 97.6%[95%CI:93.4%-99.3%] in fibrous plaque, 90.5%[95%CI:85.2%-94.1%] in lipid and 88.5%[95%CI:82.4%-92.7%] in calcium. The median time required for analysis was 21.4 (18.6-25.0) seconds per pullback. CONCLUSIONS: A novel AI framework for automatic plaque characterisation in IVOCT was developed, providing excellent diagnostic accuracy in both internal and external validation. This model might reduce subjectivity in image interpretation and facilitate IVOCT quantification of plaque composition, with potential applications in research and IVOCT-guided PCI.

4.
Small ; : e2006622, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33599046

RESUMO

The photocatalytic efficiency of polymeric carbon nitride is hampered by high carrier recombination rate and low charge transfer. Herein, these issues are addressed by constructing 1D strip-like carbon nitride with a large π-electron conjugated system from carbon-doping, realizing the synchronization control of its electronic structure and morphology. Nicotinic acid, a monomer with the carboxyl group and pyridine ring, and melamine are selected for assembling the strip-like supramolecular via hydrogen bond under hydrothermal process. Both peripheral pyridine unit and hydrogen bond have significant effect on self-assembly process of nicotinic acid and melamine along one dimension to form a strip-like precursor. Subsequently, 1D thin porous strip-like carbon nitride is obtained by calcination treatment of precursor. The as-prepared 1D strip-like carbon nitride with effective π delocalization from carbon-doping and porous structure can accelerate charges and mass transfer and provide extra active sites. Both theoretical and experimental results demonstrate that carbon doping (pyridine heterocycle) narrows the bandgap via manipulating the band position and increases the π electron density. Thus, the 1D porous thin strip-like carbon nitride realizes compelling hydrogen evolution rate (126.2 µmol h-1 ), far beyond (≈18 fold) the value of polymeric carbon nitride (PCN) (7.2 µmol h-1 ) under visible light irradiation.

5.
J Agric Food Chem ; 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33599498

RESUMO

The number and distribution of adipocytes directly affect the quality of livestock meat products. The analysis of the adipogenesis mechanism is the basis for improving meat quality. The formation of adipocytes is regulated by many factors, including a class of endogenous small RNAs, named microRNA (miRNA). Previous studies have shown that miRNAs could affect adipogenesis by post-transcriptional regulation of target genes. In our study, a decreased miR-99b-5p expression level was found in the adipose tissue of obese mice. Overexpression of miR-99b-5p could increase cell proliferation by promoting the cell cycle while inhibiting cell differentiation. In addition, interference with miR-99b-5p obtained the opposite result. Furthermore, the proteomics sequencing analysis screened 1154 differentially expressed proteins, which are closely related to adipocyte differentiation and fatty acid metabolism. In addition, the results of the dual-luciferase test showed that miR-99b-5p can directly target the proteins SCD1 and Lpin1 with significantly different expression levels in proteomic sequencing. Then, this result was verified at the level of mRNA and protein in a further study. Collectively, these results suggested that miR-99b-5p may be a target for improving meat quality.

6.
Stem Cell Res Ther ; 12(1): 132, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33588941

RESUMO

BACKGROUND: Bone morphogenetic protein 2 (BMP2) is a promising chondrogenic growth factor for cartilage tissue-engineering, but it also induces robust endochondral ossification. Human synovial-derived mesenchymal stromal cells (hSMSCs) have attracted great interest due to their poor potential for differentiation into osteogenic lineages. Smad7 plays a significant in the endochondral ossification. In this study, we explored a new method to amplify the BMP2-induced chondrogenic differentiation of hSMSCs by downregulating Smad7 and applying a cellular scaffold. METHODS: hSMSCs were isolated from human knee joint synovium from 3 donors through adhesion growth. In vitro and in vivo models of the chondrogenic differentiation of hSMSCs were established. Transgenic expression of BMP2 and silencing of Smad7 and Smad7 was achieved by adenoviral vectors. The osteogenic differentiation was detected by alkaline phosphatase staining, alizarin red staining, and RT-PCR analysis of the osteogenic genes RUNX2, Osterix, and Osteocalcin. The chondrogenic differentiation was detected by Alcian blue staining and RT-PCR analysis of the chondrogenic genes SOX9, COL2, and aggrecan. Hypertrophic differentiation was detected by the markers COL10 and MMP13. A subcutaneous stem cell implantation model was established with polyethylene glycol citrate-co-N-isopropylacrylamide (PPCN) scaffolds and athymic nude mice (3/group, 4-6 week-old female) and evaluated by micro-CT, H&E staining, and Alcian blue staining. An immunohistochemistry assay was used to detected COL1 and COL2, and an immunofluorescence assay was used to detect COL10 and MMP13. RESULTS: These hSMSCs identified by flow cytometry. These hSMSCs exhibited lower osteo-differentiation potential than iMads and C3H10T1/2-cells. When Smad7 was silenced in BMP2-induced hSMSCs, the chondrogenic differentiation genes SOX9, COL2, and aggrecan were enhanced in vitro. Additionally, it silencing Smad7 led to a decrease in the hypertrophic differentiation genes COL10 and MMP13. In subcutaneous stem cell implantation assays, immunofluorescence and immunohistochemical staining demonstrated that silencing Smad7 increased the number of COL2-positive cells and decreased the expression of COL1, COL10, and MMP13. CONCLUSION: This study suggests that the application of hSMSCs, cell scaffolds, and silencing Smad7 can potentiate BMP2-induced chondrogenic differentiation and inhibit endochondral ossification. Thus, inhibiting the expression of Smad7 in BMP2-induced hSMSC differentiation may be a new strategy for cartilage tissue-engineering.

7.
Pest Manag Sci ; 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33538074

RESUMO

BACKGROUND: Inoculation of arbuscular mycorrhizal (AM) fungi in soil can promote the uptake of nutrients and xenobiotics by plants. In this study, the effects of arbuscular mycorrhizal fungi (including Glomus itraradices and Glomus mossea) on the growth of maize, the uptake of carbosulfan and the control efficacy on S. frugiperda were investigated through maize seed coating. RESULTS: Results from the pot experiment showed that carbofuran reduced the mycorrhizal colonization of AM fungi in the early stage of the experiment. The inhibiting effect disappeared in 21-49 days, while the mycorrhizal colonization rate in the Glomus itraradices (G. itraradices) treatment was maintained at about 90%. Compared with non-inoculated treatment, the fresh weights of roots in G. itraradices and Glomus mosseae (G. mosseae) treatments increased by 20-41% and 10-23%, respectively. Mycorrhizal treatment could significantly increase the transmission rates (root/soil and leaf/stem) and the carbosulfan accumulation in maize. During the harvest period, the control efficacy against Spodoptera frugiperda (S. frugiperda) in mycorrhizal treatment was significantly higher than that in non-inoculated treatments (p < 0.05) in both Guangzhou and Nanning. CONCLUSIONS: AM inoculation could accelerate the degradation process of carbofuran in soil and the propagation of carbofuran from soil to plants. G. intraradices showed more pronounced effects than G. mosseae on both plant growth and carbosulfan content in plants and soil. The experimental results showed that inoculation of AM fungi has increased the accumulation of carbofuran in plants, improved the effective utilization rate, and enhanced the control efficacy against S. frugiperda. This article is protected by copyright. All rights reserved.

8.
J Evid Based Med ; 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33615709

RESUMO

It has been over 20 years since the introduction of evidence-based medicine (EBM) into the research of traditional Chinese medicine (TCM). The development of evidence-based TCM research has profoundly influenced the process of clinical research and decision-making, impelling researchers to pay attention to raise evidence quality, accumulate data, and explore appropriate evaluation methods adaptive to TCM original theories and knowledge. In this paper, the authors aim to summarize and review the existing work and seek promising research interests in this field, expecting to inspire more thoughts leading to breakthroughs in the near future.

9.
Small ; : e2005357, 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33615728

RESUMO

Despite numerous inherent merits of metal-organic frameworks (MOFs), structural fragility has imposed great restrictions on their wider involvement in many applications, such as in catalysis. Herein, a strategy for enhancing stability and enabling functionality in a labile Zr(IV)-MOF has been proposed by in situ porphyrin substitution. A size- and geometry-matched robust linear porphyrin ligand 4,4'-(porphyrin-5,15-diyl)dibenzolate (DCPP2- ) is selected to replace the 4,4'-(1,3,6,8-tetraoxobenzo[lmn][3,8]phenanthroline-2,7(1H,3H,6H,8H)-diyl)dibenzoate (NDIDB2- ) ligand in the synthesis of BUT-109(Zr), affording BUT-110 with varied porphyrin contents. Compared to BUT-109(Zr), the chemical stability of BUT-110 series is greatly improved. Metalloporphyrin incorporation endows BUT-110 MOFs with high catalytic activity in the photoreduction of CO2 , in the absence of photosensitizers. By tuning the metal species and porphyrin contents in BUT-110, the resulting BUT-110-50%-Co is demonstrated to be a good photocatalyst for selective CO2 -to-CO reduction, via balancing the chemical stability, photocatalytic efficiency, and synthetic cost. This work highlights the advantages of in situ ligand substitution for MOF modification, by which uniform distribution and high content of the incoming ligand are accessible in the resulting MOFs. More importantly, it provides a promising approach to convert unstable MOFs, which mainly constitute the vast MOF database but have always been neglected, into robust functional materials.

10.
Hum Vaccin Immunother ; : 1-8, 2021 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-33606578

RESUMO

Vaccinia virus was used to prevent smallpox. After the World Health Organization declared smallpox extinct, vaccinia virus has been explored for the development of vaccines against a variety of infectious diseases. It also finds a new place in oncolytic therapy. Here we provide a brief review of the history, current status, and future prospect of vaccinia virus-based vaccine and oncolytic virus. New advancements, including a single vaccine targeting multiple viruses, strategies of arming vaccinia viruses to enhance anti-tumor activity, the promise and challenge of combining vaccinia-based virotherapy with immunotherapy, are discussed as special focus.

11.
J AAPOS ; 2021 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-33621686

RESUMO

PURPOSE: To compare the surgical outcomes of modified vertical rectus belly transposition (mVRBT) and medial rectus recession (MRc) versus augmented superior rectus transposition (aSRT) and MRc in Chinese patients with chronic abducens nerve palsy. METHODS: The medical records of patients with chronic abducens nerve palsy who underwent mVRBT/MRc or aSRT/MRc were retrospectively reviewed. Pre- and postoperative deviation in primary position, pre- and postoperative abduction limitation, and complications were recorded. Follow-up was at least 6 months. RESULTS: A total of 26 patients (mean age, 37.9 ± 19.6 years; 16 males [62%]) were included. Fourteen patients underwent mVRBT/MRc (mVRBT group) and 12 underwent aSRT/MRc (aSRT group). Both groups had similar amounts of recession (t = 0.27; P = 0.79). After surgery, statistically significant changes of abduction limitation and esotropia were observed (both P < 0.05). However, the difference in abduction improvement between groups was not statistically significant (mVRBT vs aSRT, 2.3 ± 0.91 vs 2.3 ± 0.97; t = 0.10, P = 0.92). Of the 19 patients who underwent unilateral surgery, preoperative esotropia was similar in both groups (t = 1.3; P = 0.21), but more esotropia was corrected in the mVRBT group than in the aSRT group (mVRBT vs aSRT, 57.8Δ ± 14.3Δ vs 44.6Δ ± 9.8Δ; t = 2.1; P = 0.047). There was no symptomatic vertical or torsional deviation. CONCLUSIONS: In our patient cohort, mVRBT/MRc showed better effect in correcting esotropia and similar effect in improving abduction limitation compared with aSRT/MRc.

12.
J Cardiothorac Surg ; 16(1): 13, 2021 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-33468199

RESUMO

BACKGROUND: Lymph node ratio (LNR) has been suggested to be an effective prognostic tool for stratifying non-small cell lung cancer (NSCLC) cases. In this study, we sought to determine cancer-specific survival (CCS) of NSCLC cases from the SEER registry and used the X-tile method to optimize CCS-based LNR cut-off points for prognostic stratification of node-positive NSCLC. METHODS: CSS and other clinicopathologic variables were retrieved from the SEER registry. Kaplan-Meier methods were used to calculate CSS. The optimal cut-off points for LNR classification were determined by the X-tile approach. Multivariate Cox regression analysis was performed to identify independent risks of CSS. RESULTS: Totally 11,341 lung cancer patients were included. Their median CSS was 22 months (range 0,143). The median LNR was 0.22 (Q1,Q3: 0.11, 0.50). X-tile analysis showed that the optimal LNR cut-off points were 0.28 and 0.81, dividing the cohort into low (LNR1 ≤ 0.28; n = 6580, 58%), middle (0.28 < LNR2 < 0.81; n = 3025, 26.7%), and high (LNR3 > 0.81; n = 1736, 15.3%) subsets. Kaplan-Meier analysis showed that patients with a low LNR had a significantly higher CCS versus patients with middle or high LNR (P < 0.001). Multivariate competing risks regression analysis revealed that LNR was an independent and significant adverse predictor of CSS (LNR2 vs. LNR1: SHR: 1.56, 95%CI: 1.47,1.67, P < 0.001; LNR3 vs. LNR1: SHR: 2.54, 95%CI: 2.30,2.80, P < 0.001). CONCLUSIONS: LNR is an independent prognostic factor of node-positive NSCLC and its optimal cut-off values established using the robust x-tile method effectively define subpopulations of node-positive NSCLC cases, which is important in guiding selection of treatment strategies clinically.

13.
PLoS One ; 16(1): e0246163, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33481954

RESUMO

[This corrects the article DOI: 10.1371/journal.pone.0241371.].

14.
Diabetes ; 70(2): 603-615, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33472945

RESUMO

Diabetic nephropathy (DN), a vascular complication of diabetes, is the leading cause of death in patients with diabetes. The contribution of aberrantly expressed circular RNAs (circRNAs) to DN in vivo is poorly understood. Integrated comparative circRNA microarray profiling was used to examine the expression of circRNAs in diabetic kidney of db/db mice. We found that circRNA_010383 expression was markedly downregulated in diabetic kidneys, mesangial cells, and tubular epithelial cells cultured in high-glucose conditions. circRNA_010383 colocalized with miRNA-135a (miR-135a) and inhibited miR-135a function by directly binding to miR-135a. In vitro, the knockdown of circRNA_010383 promoted the accumulation of extracellular matrix (ECM) proteins and downregulated the expression of transient receptor potential cation channel, subfamily C, member 1 (TRPC1), which is a target protein of miR-135a. Furthermore, circRNA_010383 overexpression effectively inhibited the high-glucose-induced accumulation of ECM and increased TRPC1 levels in vitro. More importantly, the kidney target of circRNA_010383 overexpression inhibited proteinuria and renal fibrosis in db/db mice. Mechanistically, we identified that a loss of circRNA_010383 promoted proteinuria and renal fibrosis in DN by acting as a sponge for miR-135a. This study reveals that circRNA_010383 may be a novel therapeutic target for DN in the future.

15.
Ann Palliat Med ; 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33440978

RESUMO

BACKGROUND: This study aimed to investigate the impact of pegylated recombinant human growth hormone (PEG-rhGH) replacement therapy on glucose and lipid metabolism in children with growth hormone deficiency (GHD). METHODS: A total of 17 children with a growth hormone deficiency were treated with PEG-rhGH (trade name Juyi' Erchun) via subcutaneous injection once a week before sleep for 3 months. The doses given were 0.2 and 0.15 mg/(kg·week). The injection sites included the upper arm, the front of the thigh, and the periumbilical area of the abdominal wall. Follow-ups were conducted every 3 months after the treatment to detect the metabolic indexes of the children's blood glucose and blood lipids. Growth and development indexes, thyroid function, and other indexes were also detected regularly. The glucose and lipid metabolism indexes of each child, including fasting blood glucose, glycosylated hemoglobin, fasting insulin, total cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL), were measured before the treatment and every three months after the treatment. The total detection time was 3-30 months. RESULTS: No significant differences in fasting blood glucose, glycosylated hemoglobin, fasting insulin, total cholesterol, triglycerides, HDL, and LDL were detected after the treatment when compared with measurements taken before the treatment (P>0.05). CONCLUSIONS: PEG-rhGH replacement therapy may have no significant impact on glucose and lipid metabolism in children with GHD. However, this conclusion needs to be verified through studies with larger samples and long-term follow-up periods.

16.
Artigo em Inglês | MEDLINE | ID: mdl-33428360

RESUMO

STUDY DESIGN: An anatomical study in fifteen cadavers. OBJECTIVE: To observe the anatomical relationship of the extrapedicular puncture approach with the spinal nerve and its branches, evaluate the risk of injury to these nerves and recommend a safe puncture path for lumbar extrapedicular percutaneous vertebroplasty (PVP) and percutaneous kyphoplasty (PKP). SUMMARY OF BACKGROUND DATA: There have been no reports on the anatomical relationship of the extrapedicular approach with the spinal nerve and its branches. METHODS: The positions, diameters and paths of the anterior ramus (AR), medial branch (MB), and lateral branch (LB) outside the pedicles of L1-L4 were observed, and the related anatomical parameters were measured. Three types of extrapedicular puncture paths were simulated: puncture inside the MB, puncture outside the MB, and puncture outside the LB. By evaluating the risk of injury to the MB, LB and AR, an appropriate puncture path was recommended. RESULTS: During puncture inside the MB, the incidence of MB injury was 93.3% to 100%. During puncture outside the MB, the incidence rates of MB injury at L1 and L2 were 10.0% and 3.3%, respectively, and the incidence of LB injury at L4 was 10.0%. During puncture outside the LB, there were no cases of injury to the LB or MB. There was no incidence of AR injury during L1-L3 extrapedicular puncture, but the risk of AR injury was 3.3% when the procedure was performed at L4. The recommended technique is puncture outside the LB, where the needle vertically crosses the transverse process, slides inward, and advances while being held tightly against the bone. CONCLUSIONS: Extrapedicular puncture occurs adjacent to the spinal nerve and its branches, posing a risk of injury to these nerves. At the L1-L3 levels, puncture outside the LB can avoid damage to the spinal nerve and its branches.Level of Evidence: N/A.

17.
Pest Manag Sci ; 2021 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-33491844

RESUMO

BACKGROUND: Chemical control is the most used and effective method to control Diaphorina citri, the vector of the phloem-limited bacteria associated with citrus huanglongbing (HLB) disease. The objectives of this study were to determine the effectiveness of flupyradifurone applied via dripping irrigation systems on D. citri. Bioassays were conducted using leaves harvested on various dates post treatment, and insecticide residue in leaf tissue was quantified. RESULTS: The drip application of flupyradifurone on citrus trees provided high-level and long-term control against D. citri adult, and the median lethal concentration (LC50 ) for ingestion of flupyradifurone in D. citri was 22.22 mg kg-1 (fresh leaf). Flupyradifurone residue was detected in leaf tissue within 3 days after treatment. The measured level of flupyradifurone peaked on day 40 day after application, and then showed a steady decline in subsequent days for all three applied dosages. The amounts of flupyradifurone in upper, middle, and lower leaves were similar, and trends in the change in concentration of flupyradifurone were consistent. CONCLUSIONS: The results demonstrate that flupyradifurone can be a valuable new tool for D. citri management programs, and drip-applied flupyradifurone provides an extended period of control efficacy. This paper could provide a reference to reduce the dependence on foliar-applied insecticides, with associated benefits for non-target exposure to workers and pollinators.

18.
J Cell Mol Med ; 2021 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-33506637

RESUMO

Lung cancer is the leading cause of cancer-related death worldwide, and non-small cell lung cancer (NSCLC) accounts for 85% of lung cancer diagnoses. As an ancient therapy, moxibustion has been used to treat cancer-related symptoms in clinical practice. However, its antitumour effect on NSCLC remains largely unexplored. In the present study, a Lewis lung cancer (LLC) xenograft tumour model was established, and grain-sized moxibustion (gMoxi) was performed at the acupoint of Zusanli (ST36). Flow cytometry and RNA sequencing (RNA-Seq) were used to access the immune cell phenotype, cytotoxicity and gene expression. PK136, propranolol and epinephrine were used for natural killer (NK) cell depletion, ß-adrenoceptor blockade and activation, respectively. Results showed that gMoxi significantly inhibited LLC tumour growth. Moreover, gMoxi significantly increased the proportion, infiltration and activation of NK cells, whereas it did not affect CD4+ and CD8+ T cells. NK cell depletion reversed gMoxi-mediated tumour regression. LLC tumour RNA-Seq indicated that these effects might be related to the inhibition of adrenergic signalling. Surely, ß-blocker propranolol clearly inhibited LLC tumour growth and promoted NK cells, and gMoxi no longer increased tumour regression and promoted NK cells after propranolol treatment. Epinephrine could inhibit NK cell activity, and gMoxi significantly inhibited tumour growth and promoted NK cells after epinephrine treatment. These results demonstrated that gMoxi could promote NK cell antitumour immunity by inhibiting adrenergic signalling, suggesting that gMoxi could be used as a promising therapeutic regimen for the treatment of NSCLC, and it had a great potential in NK cell-based cancer immunotherapy.

19.
J Colloid Interface Sci ; 590: 1-11, 2021 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-33517246

RESUMO

It is still a challenge to evolve visible light photocatalysts that possess both efficient oxidation and reduction capabilities. In this paper, phosphorus-doped tubular carbon nitride@UiO-66-NH2 (p-TCN@U6-X) composites were prepared by in-situ load of UiO-66-NH2 on the surface of p-TCN based on solvothermal method, which exhibit excellent photocatalytic oxidation and reduction ability. As a result, under visible light irradiation (λ > 420 nm), the photocatalytic H2 production performance of p-TCN@U6-3 reached 2628 µmol g-1h-l, which was 8.19 and 5.36 times higher than that of p-TCN and UiO-66-NH2, respectively. Meanwhile, p-TCN@U6-3 also exhibited well selectivity rate (99%) and conversion rate (98%) for oxidative coupling of amine compounds. The high photocatalytic activities can be assigned to the improved visible light adsorption resulted from the tubular structure of p-TCN and enhanced electrical conductivity because of the phosphorus doping in p-TCN. Furthermore, UiO-66-NH2 plays the role of co-catalyst and active centers in the photocatalytic system to synergistically catalyze the reactions. Transient photocurrent spectra, steady-state photoluminescence (PL) and time-resolved photoluminescence (TRPL) further prove the more effective charge separation and transfer happened in the p-TCN@U6-X system compared with sole p-TCN and UiO-66-NH2, respectively. This work provides an effective method for creating novel carbon nitride-based photocatalytic systems with efficient capability for photocatalytic oxidation and reduction.

20.
Nanoscale Res Lett ; 16(1): 4, 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33404823

RESUMO

Conductive and degradable nanofibrous scaffolds have great potential in promoting cell growth, proliferation, and differentiation under an external electric field. Although the issue of inferior electrical conductivity in body fluids still exists, polyaniline (PANI)-based degradable nanofibers can promote cell adhesion, growth, and proliferation. To investigate whether the effect is caused by the PANI morphology, we selected three inorganic acids as dopants in the process of PANI in situ oxidative polymerization: hydrochloric acid, sulfuric acid, and perchloric acid. The obtained polyaniline/polylactic acid (PANI/PLA) composite nanofibers were characterized via SEM, FTIR, and XPS analysis, and we confirmed that the PLA nanofibers were successfully coated by PANI without any change to the porous structure of the PLA nanofibers. The in vitro mechanical properties and degradability indicated that the oxidation of acid dopants should be considered and that it was likely to have a higher oxidation degradation effect on PLA nanofibers. The contact angle test demonstrated that PANI/PLA composite nanofibers with different surface morphologies have good wettability, implying that they meet the requirements of bone tissue engineering scaffolds. The surface roughness and cell viability demonstrated that different PANI morphologies on the surface can promote cell proliferation. The higher the surface roughness of the PANI, the better the biocompatibility. Consequently, the regulated surface morphology of PANI/PLA composite nanofibers via different acids doping has positive effect on biocompatibility in tissue engineering.

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