Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 88
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Artigo em Inglês | MEDLINE | ID: mdl-34562127

RESUMO

It has been well known that the free ion concentration of metals plays a vital role in metal bioavailability. However, measurement of this fraction is still not easy over years of development. Nowadays, rare earth elements (REEs) are drawing more attentions as an emerging contaminant due to their wide applications in our daily life. To analyze the free ion concentration of neodymium (Nd), we adopted ion-exchange technique (IET) to investigate the changes on Nd free ion concentration in the presence of fulvic acid (FA). With the dynamic mode of IET analysis, the concentrations of Nd free ion were in the range of 0.85-36.8 × 10-8 M at the total Nd concentration of 5 × 10-7 M when FA varied from 0.4 to 10 M. However, these concentrations were 3-58 times higher than the one calculated by WHAM 7.0, which may be due to the particulate Nd spontaneously formed in solution. With single particle ICP-MS analysis, we found 0.25%-2.36% of Nd was in the form of colloids when the total Nd concentrations varied from 8.5 × 10-9 to 4.7 × 10-7 M, with the average particle sizes in the range of 26.5-39.2 nm. The presence of FA significantly decreased the number of Nd colloids, but increased the average particle size. Under the TEM, we found that Nd colloids were amorphous, with the size less than 200 nm. The present study provided a relatively new perspective on REE speciation in water. The natural organic matters not only affect the free ion concentration of Nd, but also influenced the size and numbers of Nd colloids in solution.

2.
Front Pharmacol ; 12: 613458, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33897415

RESUMO

Naturally occurring isothiocyanates (ITCs) from edible vegetables have shown potential as chemopreventive agents against several types of cancer. The aims of the present study were to study the potential of ITCs in chemoprevention and in potentiating the efficacy of cytotoxic drugs in gastric cancer treatment. The chemoprevention was studied in chemically induced mouse model of gastric cancer, namely N-methyl-N-nitrosourea (MNU) in drinking water, and in a genetically engineered mouse model of gastric cancer (the so-called INS-GAS mice). The pharmacological effects of ITCs with or without cisplatin were studied in human gastric cell lines MKN45, AGS, MKN74 and KATO-III, which were derived from either intestinal or diffused types of gastric carcinoma. The results showed that dietary phenethyl isothiocyanate (PEITC) reduced the tumor size when PEITC was given simultaneously with MNU, but neither when administrated after MNU nor in INS-GAS mice. Treatments of gastric cancer cells with ITCs resulted in a time- and concentration-dependent inhibition on cell proliferation. Pretreatment of gastric cancer cells with ITCs enhanced the inhibitory effects of cisplatin (but not 5-fluorouracil) in time- and concentration-dependent manners. Treatments of gastric cancer cells with PEITC plus cisplatin simultaneously at different concentrations of either PEITC or cisplatin exhibited neither additive nor synergetic inhibitory effect. Furthermore, PEITC depleted glutathione and induced G2/M cell cycle arrest in gastric cancer cells. In conclusion, the results of the present study showed that PEITC displayed anti-cancer effects, particularly when given before the tumor initiation, suggesting a chemopreventive effect in gastric cancer, and that pretreatment of PEITC potentiated the anti-cancer effects of cisplatin, possibly by reducing the intracellular pool of glutathione, suggesting a possible combination strategy of chemotherapy with pretreatment with PEITC.

3.
Front Pharmacol ; 12: 625991, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33867984

RESUMO

Objective: The aim of the present study was repositioning of ivermectin in treatment of gastric cancer (GC) by computational prediction based on gene expression profiles of human and mouse model of GC and validations with in silico, in vitro and in vivo approaches. Methods: Computational drug repositioning was performed using connectivity map (cMap) and data/pathway mining with the Ingenuity Knowledge Base. Tissue samples of GC were collected from 16 patients and 57 mice for gene expression profiling. Additional seven independent datasets of gene expression of human GC from the TCGA database were used for validation. In silico testing was performed by constructing interaction networks of ivermectin and the downstream effects in targeted signaling pathways. In vitro testing was carried out in human GC cell lines (MKN74 and KATO-III). In vivo testing was performed in a transgenic mouse model of GC (INS-GAS mice). Results: GC gene expression "signature" and data/pathway mining but not cMAP revealed nine molecular targets of ivermectin in both human and mouse GC associated with WNT/ß-catenin signaling as well as cell proliferation pathways. In silico inhibition of the targets of ivermectin and concomitant activation of ivermectin led to the inhibition of WNT/ß-catenin signaling pathway in "dose-depended" manner. In vitro, ivermectin inhibited cell proliferation in time- and concentration-depended manners, and cells were arrested in the G1 phase at IC50 and shifted to S phase arrest at >IC50. In vivo, ivermectin reduced the tumor size which was associated with inactivation of WNT/ß-catenin signaling and cell proliferation pathways and activation of cell death signaling pathways. Conclusion: Ivermectin could be recognized as a repositioning candidate in treatment of gastric cancer.

4.
iScience ; 24(2): 102091, 2021 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-33598644

RESUMO

Tumors comprise cancer cells and the associated stromal and immune/inflammatory cells, i.e., tumor microenvironment (TME). Here, we identify a metabolic signature of human and mouse model of gastric cancer and show that vagotomy in the mouse model reverses the metabolic reprogramming, reflected by metabolic switch from glutaminolysis to OXPHOS/glycolysis and normalization of the energy metabolism in cancer cells and TME. We next identify and validate SNAP25, mTOR, PDP1/α-KGDH, and glutaminolysis as drug targets and accordingly propose a therapeutic strategy to target the nerve-cancer metabolism. We demonstrate the efficacy of nerve-cancer metabolism therapy by intratumoral injection of BoNT-A (SNAP25 inhibitor) with systemic administration of RAD001 and CPI-613 but not cytotoxic drugs on overall survival in mice and show the feasibility in patients. These findings point to the importance of neural signaling in modulating the tumor metabolism and provide a rational basis for clinical translation of the potential strategy for gastric cancer.

5.
Cell Mol Gastroenterol Hepatol ; 10(2): 434-449.e1, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32330731

RESUMO

BACKGROUND & AIMS: Enterochromaffin-like (ECL) cells in the stomach express gastrin/cholecystokinin 2 receptor CCK2R and are known to expand under hypergastrinemia, but whether this results from expansion of existing ECL cells or increased production from progenitors has not been clarified. METHODS: We used mice with green fluorescent protein fluorescent reporter expression in ECL cells (histidine decarboxylase [Hdc]-green fluorescent protein), as well as Cck2r- and Hdc-driven Tamoxifen inducible recombinase Cre (Cck2r-CreERT2, Hdc-CreERT2) mice combined with Rosa26Sor-tdTomato (R26-tdTomato) mice, and studied their expression and cell fate in the gastric corpus by using models of hypergastrinemia (gastrin infusion, omeprazole treatment). RESULTS: Hdc-GFP marked the majority of ECL cells, located in the lower third of the gastric glands. Hypergastrinemia led to expansion of ECL cells that was not restricted to the gland base, and promoted cellular proliferation (Ki67) in the gastric isthmus but not in basal ECL cells. Cck2r-CreERT2 mice marked most ECL cells, as well as scattered cell types located higher up in the glands, whose number was increased during hypergastrinemia. Cck2r-CreERT2+ isthmus progenitors, but not Hdc+ mature ECL cells, were the source of ECL cell hyperplasia during hypergastrinemia and could grow as 3-dimensional spheroids in vitro. Moreover, gastrin treatment in vitro promoted sphere formation from sorted Cck2r+Hdc- cells, and increased chromogranin A and phosphorylated- extracellular signal-regulated kinase expression in CCK2R-derived organoids. Gastrin activates extracellular signal-regulated kinase pathways in vivo and in vitro, and treatment with the Mitogen-activated protein kinase kinase 1 inhibitor U0126 blocked hypergastrinemia-mediated changes, including CCK2R-derived ECL cell hyperplasia in vivo as well as sphere formation and chromogranin A expression in vitro. CONCLUSIONS: We show here that hypergastrinemia induces ECL cell hyperplasia that is derived primarily from CCK2R+ progenitors in the corpus. Gastrin-dependent function of CCK2R+ progenitors is regulated by the extracellular signal-regulated kinase pathway.


Assuntos
Celulas Tipo Enterocromafim/patologia , Mucosa Gástrica/patologia , Gastrinas/sangue , Animais , Modelos Animais de Doenças , Celulas Tipo Enterocromafim/metabolismo , Mucosa Gástrica/citologia , Mucosa Gástrica/metabolismo , Gastrinas/metabolismo , Humanos , Hiperplasia/sangue , Hiperplasia/patologia , Sistema de Sinalização das MAP Quinases , Camundongos , Receptor de Colecistocinina B/metabolismo , Células-Tronco/patologia
6.
Chin J Integr Med ; 26(8): 609-616, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31705448

RESUMO

OBJECTIVE: To evaluate the efficacy of electro-acupuncture (EA) or transcutaneous electrical acupoint stimulation (TEAS) on perioperative cerebral blood flow (CBF) and neurological function in ischemic stroke (IS) patients undergoing carotid artery stenting (CAS). METHODS: In total, 124 consecutive IS patients were randomly allocated to the EA, TEAS, and sham groups (groups A, T, and S; 41, 42, and 41 cases, respectively) by software-derived random-number sequence. Groups A and T received EA and TEAS, respectively, at the Shuigou (GV 26) and Baihui (GV 20), Hegu (LI4) and Waiguan (TE 5) acupoints. Group S received sham EA. The stimulation was started from 30 min before surgery until the end of the operation. The primary outcome was the CBF at 30 min after surgery, which was measured by transcranial Doppler sonography. The secondary outcomes included hyperperfusion incidence and neurological function. National Institutes of Health Stroke Scale (NIHSS) and General Evaluation Scale (GES) scores were recorded at 1 week, 1 month, and 3 months postoperatively. RESULTS: Mean CBF velocity at 30 min after surgery in groups A and T was much lower than that in Group S (P < 0.05); the incidence of hyperperfusion in Groups A and T was also lower than that in group S (P <0.05). Acupuncture was an independent factor associated with reduced incidence of hyperperfusion (OR=0.042; 95% CI: 0.002-0.785; =0.034). NIHSS and GES scores improved significantly at 1 week postoperatively in Groups A and T than in Group S (P < 0.05). Relative to Group S, groups A and T exhibited significantly lower incidences of moderate pain, as well as higher incidences of satisfaction with anesthesia, at 1 day postoperatively (P < 0.05). CONCLUSIONS: EA or TEAS administered in combination with local anesthesia during CAS can inhibit transient increases in CBF, reduce the incidence of postoperative hyperperfusion, and improve neurological function. (Registration No. ChiCTR-IOR-15007447).


Assuntos
Anestesia Local , Doenças das Artérias Carótidas/terapia , Circulação Cerebrovascular/fisiologia , Eletroacupuntura/métodos , Procedimentos Endovasculares , AVC Isquêmico/terapia , Idoso , Terapia Combinada , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medição da Dor , Estudos Prospectivos , Traumatismo por Reperfusão/epidemiologia , Stents
7.
Bull Environ Contam Toxicol ; 103(4): 565-570, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31410500

RESUMO

Nowadays rare earth elements (REEs) are widely applied in high-technology and clean energy products, but their environmental risks are still largely unknown. To estimate the ecological risk of REEs, soil samples were collected from REE mine tailings with and without phytoremediation. The results showed that the tailings had rather low organic matter and high total REE concentrations, up to 808.5 mg/kg. The 10% effective concentration (EC10) of neodymium (Nd) and yttrium (Y) were calculated based on the toxicity tests of seed germination and root growth. For both wheat and mung bean, the EC10 of Nd and Y in soils were in the range of 1053.1-1300.1 mg/kg. The average hazard quotient of mine tailing soil without phytoremediation was higher than that with phytoremediation. All the hazard quotient of Nd and Y were less than 1, indicating that Nd or Y alone was unlikely to cause adverse ecological effects. Given to the coexistence of REEs on mine sites, the ecological risk of REE mixture could be potentially high towards local soil environments, even for soils with phytoremdiation.


Assuntos
Mineração , Neodímio/análise , Poluentes do Solo/análise , Solo/química , Ítrio/análise , Biodegradação Ambiental , China , Medição de Risco , Triticum/química , Triticum/crescimento & desenvolvimento , Vigna/química , Vigna/crescimento & desenvolvimento
8.
Sci Total Environ ; 696: 134004, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31465922

RESUMO

Although many studies have addressed the effects of various physicochemical and biological factors on metal bioaccumulation in aquatic organisms, the influences of metal exposure history have drawn much less attention. In the present study, we investigated the effects of different Zn exposure regimes (concentration: 1-200 µg/L, duration: 1-7 d) on the subsequent biokinetics, metallothionien-like protein (MTLP) induction and subcellular distribution of Cd and Zn in Daphnia magna. Zn body burden increased significantly with elevated exposure concentrations, but was kept within a narrow range regardless of the 200-fold variation of Zn concentrations. Significant induction (7-14 folds) of MTLP by Zn exposure was evident, but was decoupled from the Zn body accumulation. Under different regimes of Zn exposure, Zn was evenly distributed in insoluble (cellular debris, intracellular organelles and metal-rich granules) and soluble fractions (heat sensitive protein and MTLP). However, >60% of Cd was bound with MTLP regardless of the exposure concentration and duration. The biokinetic processes including uptake from the dissolved phase, food assimilation and excretion of Cd and Zn were quantified with radioactive tracers. The uptake rate constants of Cd and Zn from the dissolved phase either remained comparable or increased following Zn pre-exposure, and the efflux of Zn increased by nearly two-fold to counteract the increased uptake from water. The dietary assimilation of Zn also decreased significantly in response to Zn exposure. However, Cd assimilation efficiency was kept relatively constant in each Zn pre-exposure regime. Our results showed that Zn exposure had significant influences on the biokinetics and physiology of daphnids. Daphnids attempted to maintain the Zn body burden within a narrow range by modification of biokinetic processes instead of subcellular distribution.


Assuntos
Daphnia/metabolismo , Política Ambiental , Poluentes Químicos da Água/metabolismo , Zinco/metabolismo , Animais , Carga Corporal (Radioterapia) , Cádmio , Exposição Ambiental , Metais
9.
BMC Anesthesiol ; 19(1): 53, 2019 04 11.
Artigo em Inglês | MEDLINE | ID: mdl-30971217

RESUMO

BACKGROUND: Pain is often observed in pediatric patients after craniotomy procedures, which could lead to some serious postoperative complications. However, the optimal formula for postoperative analgesia for pediatric neurosurgery has not been well established. This study aimed to explore the optimal options and formulas for postoperative analgesia in pediatric neurosurgery. METHODS: Three hundred and twenty patients aged 1 to 12-years old who underwent craniotomy were randomly assigned to receive 4 different regimens of patient-controlled analgesia. The formulas used were as follows: Control group included normal saline 100 ml, with a background infusion of 2 ml/h, bolus 0.5 ml; Fentanyl group was used with a background infusion of 0.1-0.2 µg/k·h, bolus 0.1-0.2 µg/kg; Morphine group was used with a background infusion of 10-20 µg/kg·h, bolus 10-20 µg/kg; while Tramadol group was used with a background infusion of 100-400 µg/kg·h, bolus 100-200 µg/kg. Postoperative pain scores and analgesia-related complication were recorded respectively. Comparative analysis was performed between the four groups. RESULTS: In comparison of all groups with each other, lower pain scores were shown at 1 h and 8 h after surgery in Morphine group versus Tramadol, Fentanyl and Control groups (P < 0.05). Both Tramadol and Fentanyl groups showed lower pain scores in comparison to Control group (P < 0.05). Nausea and vomiting were observed more in Tramadol group in comparison to all other groups during the 48 h of PCIA usage after operation (P = 0.020). Much more rescue medicines including ibuprofen and morphine were used in Control group (CI = 0.000-0.019). Changes in consciousness and respiratory depression were not observed in study groups. Moderate-to-severe pain was observed in a total of 56 (17.5%) of the study population. Multiple regression analysis for identifying risk factors for moderate-to-severe pain revealed that, younger children (OR = 1.161, 1.027-1.312, P = 0.017), occipital craniotomy (OR = 0.374, 0.155-0.905, P = 0.029), and morphine treatment (OR = 0.077, 0.021-0.281, P < 0.001) are the relevant factors. CONCLUSIONS: Compared with other analgesic projects, PCIA or NCIA analgesia with morphine appears to be the safest and most effective postoperative analgesia program for pediatric patients who underwent neurosurgical operations. TRIAL REGISTRATION: Chinese Clinical Trial Registry. No: ChiCTR-IOC-15007676. Prospective registration. http://www.chictr.org.cn/index.aspx .


Assuntos
Analgesia Controlada pelo Paciente/métodos , Analgésicos/administração & dosagem , Craniotomia/efeitos adversos , Manejo da Dor/métodos , Medição da Dor/métodos , Dor Pós-Operatória/tratamento farmacológico , Analgesia Controlada pelo Paciente/tendências , Criança , Pré-Escolar , Estudos de Coortes , Craniotomia/tendências , Feminino , Humanos , Lactente , Masculino , Manejo da Dor/tendências , Medição da Dor/efeitos dos fármacos , Medição da Dor/tendências , Dor Pós-Operatória/etiologia , Estudos Prospectivos , Método Simples-Cego
10.
Sci Total Environ ; 660: 697-704, 2019 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-30743955

RESUMO

Rare-earth elements (REEs) are known to be a group of emerging pollutants, but the geochemistry of REEs in river waters in ion-adsorption rare-earth mining areas has attracted little attention. In this study, samples of the <0.45 µm and 0.22-0.45 µm (large colloids) water fractions and acid-soluble particles (ASPs) were collected from a river impacted by ion-adsorption rare-earth mining activities. The roles of ligand complexation, colloid binding, and particle adsorption in REE transport and distribution were also investigated. Results showed higher concentrations of REEs in the <0.45 µm fraction of all sampling sites (3.30 × 10-2-9.42 µM) compared with that in the control site (1.21 × 10-3 µM); this fraction was also characterized by middle REE enrichment at upstream sites, where REEs are mainly controlled by the <0.22 µm fraction (55%-94% of the species found in the <0.45 µm fraction) and ligand complexation (REE3+, REE(SO4)+, and REE(CO3)+). At downstream sites, heavy REE enrichment was observed, which was largely determined by binding to large colloids (68%-83% of the species found in the <0.45 µm fraction) and adsorption to particles (>90% of the acidified bulk water). Furthermore, REE patterns indicated that the REE-associated large colloids were mineral or mixed mineral-organic matter (OM) at upstream sites and OM-dominated or functionalized at downstream sites. The particles were mainly coated by inorganic matter substances (e.g., Fe/Al oxyhydroxides). In summary, our results reveal that REE patterns provide a useful tool to study the fate of REEs in ion-adsorption rare-earth mining catchments.

11.
EBioMedicine ; 40: 67-76, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30639417

RESUMO

BACKGROUND: Bariatric surgery leads to early and long-lasting remission of type 2 diabetes (T2D). However, the mechanisms behind this phenomenon remain unclear. Among several factors, gut hormones are thought to be crucial mediators of this effect. Unlike GLP-1, the role of the hormone peptide tyrosine tyrosine (PYY) in bariatric surgery in humans has been limited to appetite regulation and its impact on pancreatic islet secretory function and glucose metabolism remains under-studied. METHODS: Changes in PYY concentrations were examined in obese patients after bariatric surgery and compared to healthy controls. Human pancreatic islet function was tested upon treatment with sera from patients before and after the surgery, in presence or absence of PYY. Alterations in intra-islet PYY release and insulin secretion were analysed after stimulation with short chain fatty acids (SCFAs), bile acids and the cytokine IL-22. FINDINGS: We demonstrate that PYY is a key effector of the early recovery of impaired glucose-mediated insulin and glucagon secretion in bariatric surgery. We establish that the short chain fatty acid propionate and bile acids, which are elevated after surgery, can trigger PYY release not only from enteroendocrine cells but also from human pancreatic islets. In addition, we identify IL-22 as a new factor which is modulated by bariatric surgery in humans and which directly regulates PYY expression and release. INTERPRETATION: This study shows that some major metabolic benefits of bariatric surgery can be emulated ex vivo. Our findings are expected to have a direct impact on the development of new non-surgical therapy for T2D correction.


Assuntos
Diabetes Mellitus Tipo 2/metabolismo , Peptídeo YY/metabolismo , Animais , Cirurgia Bariátrica , Biomarcadores , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/terapia , Células Enteroendócrinas/metabolismo , Feminino , Expressão Gênica , Peptídeo 1 Semelhante ao Glucagon/metabolismo , Humanos , Interleucinas/metabolismo , Ilhotas Pancreáticas/metabolismo , Masculino , Camundongos , Peptídeo YY/sangue , Peptídeo YY/genética , Ratos
12.
Chemosphere ; 216: 75-83, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30359919

RESUMO

Due to their specific properties, ion-adsorption rare earth mine sites may be a threat for adjacent environments. This work was undertaken to assess whether former mining operations on ion-adsorption rare earth mine sites have a significant impact on water bodies and soils of the surrounding environments. Tailing soil materials, stream waters and sediments, and farmland soils were collected from one of the largest ion-adsorption rare earth mine sites worldwide (Southern China). Total concentrations of rare earth elements (REEs), Fe, Al, etc., and pH were measured. Results revealed high concentrations of REEs in tailing soils (392 mg kg-1), stream waters (4460 µg L-1), sediments (462 mg kg-1) and farmland soils (928 mg kg-1) in comparison with control sites. In the tailing profiles, light REEs (LREEs) were preferentially leached compared to middle REEs (MREEs) and heavy REEs (HREEs). Anomalies in tailings and stream water indicated strong soil weathering (Eu) and leaching activities (Ce) within the tailings. The MREE enriched pattern in stream water was more related to water parameters such as Al and Fe oxides, and ligands, than to the source of REEs. Anomalies also indicated that REEs contamination in the farmland soils was mainly originated from the stream water contaminated by the leaching from the tailings. In conclusion, a heavy REEs pollution was recorded in the surrounding environment of ion-adsorption rare earth mine. REEs fractionation, Ce and Eu anomalies provided an insight to the understanding of REEs leaching and soil weathering processes, and REEs environmental fate in rare earth mining area.


Assuntos
Monitoramento Ambiental/métodos , Metais Terras Raras/análise , Mineração , Adsorção , Agricultura , China , Poluição Ambiental/análise , Rios , Poluentes do Solo/análise , Poluentes Químicos da Água/análise
13.
Curr Pharm Des ; 24(18): 1926-1935, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29898650

RESUMO

The number of people who are overweight and obese are continuously increasing both in the adult and adolescent populations. Coinciding with this is the increased prevalence of health problems such as type 2 diabetes (T2D). Bariatric surgery is the only proven long-term treatment of obesity and may induce remission of T2D, although the underlying mechanisms are unknown. The translational studies presented here might provide insight on the mechanism of steady-state energy balance of the obese phenotype using a special time-restricted feeding regimen for weight loss during the steady-state energy balance; mechanism by vagal blocking therapy (vBLoc® therapy) as a new treatment for obesity; and possible mechanism behind the remission of T2D following gastric bypass surgery.


Assuntos
Diabetes Mellitus Tipo 2/cirurgia , Modelos Animais de Doenças , Obesidade/cirurgia , Perda de Peso , Animais , Cirurgia Bariátrica
14.
J Clin Nurs ; 27(13-14): 2590-2597, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28618046

RESUMO

AIMS AND OBJECTIVES: To observe the effects of bundle care on preventing unplanned extubation of nasobiliary drainage catheter after endoscopic retrograde cholangiopancreatography. BACKGROUND: Preventing unplanned extubation has become a difficult problem for nursing staff because the catheter is stiff, fine and long. DESIGN: A total of 114 cases that experienced nasobiliary drainage after endoscopic retrograde cholangiopancreatography for the first time in our hospital from April 2015-July 2016 were enrolled in this study. According to receiving routine nurse or bundle nurse, these cases were randomly divided into control (n = 56) and intervention (n = 58) group. METHOD: The unplanned extubation incidence, contact area between tape and catheter and tensile resistance were compared between the two groups. RESULTS: The contact area was one square centimetre in the control group and 5 cm2 in the intervention group. Tensile resistance was significantly higher in the intervention group than in the control (all p < .05). Unplanned extubation incidence was significantly lower in the intervention group (1.72%, 1/58) than in the control (12.5%, 7/56) (p = .0305). CONCLUSION: Bundle care can effectively decrease unplanned extubation incidence after endoscopic retrograde cholangiopancreatography. RELEVANCE TO CLINICAL PRACTICE: This study provides a basis for decreasing unplanned extubation incidence.


Assuntos
Extubação/métodos , Colangiopancreatografia Retrógrada Endoscópica/efeitos adversos , Colangiopancreatografia Retrógrada Endoscópica/métodos , Drenagem/métodos , Intubação Gastrointestinal/normas , Pacotes de Assistência ao Paciente/métodos , Guias de Prática Clínica como Assunto , Idoso , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
15.
Hum Pathol ; 71: 135-144, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29126833

RESUMO

The role of BRCA1 and BRCA2 genes is mainly to maintain genome integrity in response to DNA damage through different mechanisms. Deregulation of BRCA1 and BRCA2 is associated with the development of tumor and altered sensitivity to chemotherapeutic agents. In this study, we determined protein expression of BRCA1 and BRCA2 in 4 digestive system cancers (gastric cancer, colorectal cancer, hepatocellular carcinoma, and pancreatic cancer) by immunohistochemistry on tissue microarrays. A total of 1546 samples of 4 types of cancer tissues, their matched adjacent nontumor tissues, and corresponding benign tissues were studied, respectively. Immunohistochemistry expression patterns of the 2 proteins and their correlation with patients' clinical parameters and overall survival were analyzed. The results showed that low expression of cytoplasmic BRCA1 and BRCA2 was commonly associated with advanced tumor-lymph node-metastasis stage, whereas high expression of nuclear BRCA1 was generally correlated with advanced tumor stages in these cancers. High expression of cytoplasmic BRCA1 and BRCA2 had significantly favorable overall survival in digestive system cancers; in contrast, BRCA1 nuclear expression usually predicted poor outcomes. We conclude that BRCA1 and BRCA2 could be used as clinicopathological biomarkers to evaluate the prognosis of digestive system cancers.


Assuntos
Proteína BRCA1/biossíntese , Proteína BRCA2/biossíntese , Biomarcadores Tumorais/análise , Neoplasias do Sistema Digestório/diagnóstico , Adulto , Idoso , Proteína BRCA1/análise , Proteína BRCA2/análise , Neoplasias do Sistema Digestório/mortalidade , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Prognóstico
16.
Physiol Behav ; 173: 298-304, 2017 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-28242469

RESUMO

A recent study reported that a special weekly scheduled time-restricted feeding regimen (TRF), i.e., no food consumption for 15h during the light (inactive) phase per day for 5 weekdays, attenuated the outcome of diverse nutritional challenges in response to high-fat diet in mice. In the present study, we wanted to further test whether this TRF could restrict body weight gain in both juvenile and adult animals when fed a high-fat diet. Fifty male Sprague-Dawley rats at ages from 5 to 27weeks were used. First, we found that freely fed rats with 60% fat diet gained weight significantly, which was associated with more calorie intake (particularly during light phase) than those fed standard food (7% fat). Secondly, we found that TRF restricted high-fat diet-induced weight gain in both groups of juvenile rats (5 and 13weeks of age) compared to freely fed rats with high-fat diet, despite the same levels of 24h-calorie intake during either weekdays or the weekend. Thirdly, we found that TRF did not restrict high-fat diet-induce weight gain in adult rats (27weeks of age). Thus, we suggest that this special TRF regimen could be further tested in humans (particularly young adults) for the purpose of obesity prevention.


Assuntos
Ritmo Circadiano/fisiologia , Dieta Hiperlipídica/efeitos adversos , Privação de Alimentos/fisiologia , Obesidade/etiologia , Ganho de Peso/fisiologia , Fatores Etários , Análise de Variância , Animais , Composição Corporal/fisiologia , Modelos Animais de Doenças , Ingestão de Alimentos/fisiologia , Metabolismo Energético , Comportamento Alimentar/fisiologia , Masculino , Ratos , Ratos Sprague-Dawley , Fatores de Tempo
18.
Obes Surg ; 27(1): 177-185, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-27576578

RESUMO

BACKGROUND: Recently, the US FDA has approved "vagal blocking therapy or vBLoc® therapy" as a new treatment for obesity. The aim of the present study was to study the mechanism-of-action of "VBLOC" in rat models. METHODS: Rats were implanted with VBLOC, an intra-abdominal electrical device with leads placed around gastric vagal trunks through an abdominal incision and controlled by wireless device. Body weight, food intake, hunger/satiety, and metabolic parameters were monitored by a comprehensive laboratory animal monitoring system. Brain-gut responses were analyzed physiologically. RESULTS: VBLOC reduced body weight and food intake, which was associated with increased satiety but not with decreased hunger. Brain activities in response to VBLOC included increased gene expression of leptin and CCKb receptors, interleukin-1ß, tumor necrosis factor, and transforming growth factor ß1 in the brainstem; increased CCK, somatostatin, and tyrosine hydroxylase in the hippocampus; increased NPY, AgRP, and Foxa2 in the hypothalamus; and reduced CCKb receptor, melanocortin 4 receptor, and insulin receptor in the hypothalamus. Plasma concentrations of CCK, gastrin, glucagon, GLP-1, and PYY and gastric acid secretion were unchanged in response to VBLOC. CONCLUSIONS: Based on the present study, we may suggest that VBLOC induces satiety through vagal signaling, leading to reduced food intake and loss of body weight.

19.
Cancer Cell ; 31(1): 21-34, 2017 01 09.
Artigo em Inglês | MEDLINE | ID: mdl-27989802

RESUMO

Within the gastrointestinal stem cell niche, nerves help to regulate both normal and neoplastic stem cell dynamics. Here, we reveal the mechanisms underlying the cancer-nerve partnership. We find that Dclk1+ tuft cells and nerves are the main sources of acetylcholine (ACh) within the gastric mucosa. Cholinergic stimulation of the gastric epithelium induced nerve growth factor (NGF) expression, and in turn NGF overexpression within gastric epithelium expanded enteric nerves and promoted carcinogenesis. Ablation of Dclk1+ cells or blockade of NGF/Trk signaling inhibited epithelial proliferation and tumorigenesis in an ACh muscarinic receptor-3 (M3R)-dependent manner, in part through suppression of yes-associated protein (YAP) function. This feedforward ACh-NGF axis activates the gastric cancer niche and offers a compelling target for tumor treatment and prevention.


Assuntos
Acetilcolina/fisiologia , Fator de Crescimento Neural/fisiologia , Transdução de Sinais/fisiologia , Neoplasias Gástricas/etiologia , Proteínas Adaptadoras de Transdução de Sinal/fisiologia , Animais , Proteínas de Ciclo Celular , Mucosa Gástrica/inervação , Camundongos , Camundongos Endogâmicos C57BL , Fosfoproteínas/fisiologia , Receptor Muscarínico M3/fisiologia
20.
Scand J Gastroenterol ; 52(4): 442-449, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27996337

RESUMO

OBJECTIVE: We wanted to exam the steady-state energy balance by using high-fat diet-induced obese (DIO) rats and mice as models for positive energy balance, and gastric bypassed (GB) rats and gene knockout of muscarinic acetylcholine M3 receptor (M3KO) mice as models for negative energy balance. METHODS: One hundred and thirty-two rats and mice were used. Energy balance was measured by a comprehensive laboratory animal monitoring system. Gene expression was analysed by in situ hybridisation in M3KO mice. RESULTS: DIO rats reached the plateau of body weight 28 weeks after starting high-fat diet (25% heavier than controls), whereas DIO mice reached the plateau after 6 weeks (23% heavier than controls). At the plateau, DIO rats had higher calorie intake during the light phase but not during the dark phase, while mice had the same calorie intake per day as controls. DIO rats and mice had lower energy expenditure (EE) and respiratory exchange ratio (RER) than controls. GB-rats reached the plateau (15% weight loss) 2 weeks after surgery and had the same calorie intake as sham-operated controls. EE, but not RER, was higher in GB rats than controls during the dark phase. The lean M3KO mice (25% lighter than wild-type (WT) mice at the plateau between 6 and 15 months of age) had the same calorie intake but higher EE, RER and hypothalamic mRNA expression of NPY, AgRP and leptin receptor than WT mice. CONCLUSION: When body weight gain or loss reached a plateau, the steady-state energy balance was mainly maintained by EE and/or RER rather than calorie intake.


Assuntos
Metabolismo Energético , Obesidade/metabolismo , Obesidade/cirurgia , Perda de Peso , Animais , Encéfalo/patologia , Gorduras na Dieta , Modelos Animais de Doenças , Feminino , Derivação Gástrica , Hibridização In Situ , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Obesos , RNA Mensageiro/análise , Ratos , Ratos Sprague-Dawley , Receptor Muscarínico M3/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...