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1.
Immunotherapy ; 2022 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-35574588

RESUMO

Aim: VEGF/VEGFR inhibitors may help immune checkpoint inhibitors expand the population that will benefit from treatment. The authors investigated the efficacy of combined bevacizumab and PD-1 antibody. Materials & methods: C57BL/6J mice were injected subcutaneously with 1 × 106 Lewis lung carcinoma cells. The mice were intraperitoneally injected with 0.25 mg anti-PD-1 inhibitors and/or 15 mg/kg bevacizumab. Tumor tissues were harvested. The authors reported that a non-small cell lung cancer patient received 200 mg PD-1 antibody combined with 7.5 mg/kg bevacizumab as fourth-line treatment. Results: Bevacizumab combined with PD-1 antibody induced a strong and durable antitumor effect. Bevacizumab combined with PD-1 antibody improved abnormal tumor vessels and enhanced the cytotoxic function and infiltration of T lymphocytes. The patient's survival time was significantly prolonged. Conclusion: Bevacizumab combined with anti-PD-1 antibody induces a durable antitumor effect by increasing the infiltration and cytotoxic function of CD8+ T cells in lung cancer.


Immune checkpoint inhibitors can improve long-term survival in advanced non-small-cell lung cancer, but the promising clinical benefit was only observed in a minority of patients. The combination of VEGF/VEGFR inhibitors and immune checkpoint inhibitors has become an attractive strategy. In this study, lung carcinoma mouse models were used to investigate the therapeutic efficacy of combined bevacizumab and anti-PD-1 antibody. The authors demonstrated that the combined application of bevacizumab and PD-1 blockade displays durable treatment effects by increasing the infiltration and cytotoxic function of CD8+ T cells. This study also reports a non-small-cell lung cancer patient who benefited from this treatment regimen as fourth-line therapy.

2.
Ren Fail ; 44(1): 777-789, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35535511

RESUMO

OBJECTIVE: To evaluate whether goal-directed fluid therapy (GDFT) reduces the risk of renal injury in critical illness. METHODS: MEDLINE via PubMed, EMBASE, CENTRAL and CBM was searched from inception to 13 March 2022, for studies comparing the effect of GDFT with usual care on renal function in critically ill patients. GDFT was defined as a protocolized intervention based on hemodynamic and/or oxygen delivery parameters. A fixed or random effects model was applied to calculate the pooled odds ratio (OR) based on heterogeneity through the included studies. RESULTS: A total of 28 studies with 9,019 patients were included. The pooled data showed that compared with usual care, GDFT reduced the incidence of acute kidney injury (AKI) in critical illness (OR 0.62, 95% confidence interval (CI) 0.47 to 0.80, p< 0.001). Sensitivity analysis with only low risk of bias studies showed the same result. Subgroup analyses found that GDFT was associated with a lower AKI incidence in both postoperative and medical patients. The reduction was significant in GDFT aimed at dynamic indicators. However, no significant difference was found between groups in RRT support (OR 0.88, 95% CI 0.74 to 1.05, p= 0.17). GDFT tended to increase fluid administration within the first 6 h, decrease fluid administration after 24 h, and was associated with more vasopressor requirements. CONCLUSIONS: This meta-analysis suggests that GDFT aimed at dynamic indicators may be an effective way to prevent AKI in critical illness. This may indicate a benefit from early adequate fluid resuscitation and the combined effect of vasopressors.


Assuntos
Injúria Renal Aguda , Estado Terminal , Injúria Renal Aguda/complicações , Injúria Renal Aguda/prevenção & controle , Estado Terminal/terapia , Feminino , Hidratação , Objetivos , Humanos , Rim/fisiologia , Masculino , Complicações Pós-Operatórias/epidemiologia
3.
Adv Sci (Weinh) ; : e2200393, 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35561063

RESUMO

Solution processable quasi-2D (Q-2D) perovskite materials are emerging as a promising candidate for blue light source in full-color display applications due to their good color saturation property, high brightness, and spectral tunability. Herein, an efficient energy cascade channel is developed by introducing sodium bromide (NaBr) in phenyl-butylammonium (PBA)-containing mixed-halide Q-2D perovskites for a blue perovskite light-emitting diode (PeLED). The incorporation of alkali metal contributes to the nucleation and growth of Q-2D perovskites into graded distribution of domains with different layer number . The study of excitation dynamics by transient absorption (TA) spectroscopy confirms that NaBr induces more Q-2D perovskite phases with small n number, providing a graded energy cascade pathway to facilitate more efficient energy transfer processes. In addition, the nonradiative recombination within the Q-2D perovskites is significantly suppressed upon Na+ incorporation, as validated by the trap density estimation. Consequently, the optimized blue PeLEDs manifest a peak external quantum efficiency (EQE) of 7.0% emitting at 486 nm with a maximum luminance of 1699 cd m-2 . It is anticipated that these findings will improve the understanding of alkali-metal-assisted optimization of Q-2D perovskites and pave the way toward high-performance blue PeLEDs.

4.
Stem Cell Res Ther ; 13(1): 175, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35505438

RESUMO

Stem cells are distinctive cells that have self-renewal potential and unique ability to differentiate into multiple functional cells. Stem cell is a frontier field of life science research and has always been a hot spot in biomedical research. Recent studies have shown that long non-coding RNAs (lncRNAs) have irreplaceable roles in stem cell self-renewal and differentiation. LncRNAs play crucial roles in stem cells through a variety of regulatory mechanisms, including the recruitment of RNA-binding proteins (RBPs) to affect the stability of their mRNAs or the expression of downstream genes. RBPs interact with different RNAs to regulate gene expression at transcriptional and post-transcriptional levels and play important roles in determining the fate of stem cells. In this review, the functions of lncRNAs and their RBPs in self-renewal and differentiation of stem cell are summarized. We focus on the four regulatory mechanisms by which lncRNAs and their RBPs are involved in epigenetic regulation, signaling pathway regulation, splicing, mRNA stability and subcellular localization and further discuss other noncoding RNAs (ncRNAs) and their RBPs in the fate of stem cells. This work provides a more comprehensive understanding of the roles of lncRNAs in determining the fate of stem cells, and a further understanding of their regulatory mechanisms will provide a theoretical basis for the development of clinical regenerative medicine.


Assuntos
RNA Longo não Codificante , Epigênese Genética , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , RNA não Traduzido/metabolismo , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Células-Tronco/metabolismo
5.
Plants (Basel) ; 11(8)2022 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-35448768

RESUMO

The multi-system of electro-phytotechnology using a woody ornamental cadmium (Cd) hyperaccumulator (Lonicera japonica Thunb.) is a new departure for environmental remediation. The effects of four electric field conditions on Cd accumulation, growth, and photosynthesis of L. japonica under four Cd treatments were investigated. Under 25 and 50 mg L-1 Cd treatments, Cd accumulation in L. japonica was enhanced significantly compared to the control and reached 1110.79 mg kg-1 in root and 428.67 mg kg-1 in shoots influenced by the electric field, especially at 2 V cm-1, and with higher bioaccumulation coefficient (BC), translocation factor (TF), removal efficiency (RE), and the maximum Cd uptake, indicating that 2 V cm-1 voltage may be the most suitable electric field for consolidating Cd-hyperaccumulator ability. It is accompanied by increased root and shoots biomass and photosynthetic parameters through the electric field effect. These results show that a suitable electric field may improve the growth, hyperaccumulation, and photosynthetic ability of L.japonica. Meanwhile, low Cd supply (5 mg L-1) and medium voltage (2 V cm-1) improved plant growth and photosynthetic capacity, conducive to the practical application to a plant facing low concentration Cd contamination in the real environment.

6.
Nanomaterials (Basel) ; 12(8)2022 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-35457955

RESUMO

Vibration is a common phenomenon in various fields which can not only indicate the working condition of the installation, but also serve as an energy source if it is efficiently harvested. In this work, a robust silicone rubber strip-based triboelectric nanogenerator (SRS-TENG) for vibration energy harvesting and multi-functional self-powered sensing is proposed and systematically investigated. The SRS-TENG consists of a silicone rubber strip and two aluminum electrode layers supported by polylactic acid (PLA), and acts as a sustainable power source and vibration frequency, amplitude and acceleration sensor as well. The soft contact between the aluminum electrode and silicone rubber strip makes it robust and stable even after 14 days. It can be applied in ranges of vibration frequencies from 5 to 90 Hz, and amplitudes from 0.5 to 9 mm, which shows it has advantages in broadband vibration. Additionally, it can achieve lower startup limits due to its soft structure and being able to work in multi-mode. The output power density of the SRS-TENG can reach 94.95 W/m3, matching a resistance of 250 MΩ, and it can light up more than 100 LEDs and power a commercial temperature sensor after charging capacitors. In addition, the vibration amplitude can be successfully detected and displayed on a human-machine interface. Moreover, the frequency beyond a specific limit can be distinguished by the SRS-TENG as well. Therefore, the SRS-TENG can be utilized as an in situ power source for distributed sensor nodes and a multifunctional self-powered vibration sensor in many scenarios.

7.
J Surg Res ; 277: 67-75, 2022 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-35468403

RESUMO

INTRODUCTION: In clinical practice, some patients undergoing surgery for thymoma require post-surgical ventilator support, although, factors associated with administration of ventilator support are unclear. This study aimed to explore factors associated with incidence of post-surgical severe respiratory failure requiring ventilator support after thymoma resection. METHODS: Clinical data of patients who underwent thymoma re-section in our thoracic surgery department between January 2001 and February 2020 was retrospectively analyzed. Multiple logistic regression analysis was used to identify factors associated with patient need for post-surgical ventilator support after thymoma resection. RESULTS: Among 157 patients who underwent thymoma resection, 17.8% (28/157) required post-surgical ventilator support. Results of univariate analysis revealed that gender, myasthenia gravis (MG) grade, anti-MG medication use (neostigmine or prednisone), Masaoka thymoma stage, pulmonary function test index values, surgical approach, and intraoperative blood loss were associated with increased incidence of severe respiratory failure requiring post-operative ventilator support (P < 0.05). Results of multivariable logistic regression analysis revealed that median sternotomy, MG grade three status, and patient use of anti-MG drug treatments before thymoma resection surgery were associated with greater need for post-surgical ventilator support. CONCLUSIONS: Our data suggest that median sternotomy, MG grade three status, and preoperative use of anti-MG drugs are associated with greater incidence of severe respiratory failure requiring respiratory support after thymoma surgery. Therefore, patients with these risk factors should be closely monitored to reduce the incidence of severe postoperative respiratory failure.

8.
Sci Rep ; 12(1): 4424, 2022 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-35292742

RESUMO

In this study, red mud modified by manganese dioxide(MRM) was utilized as an adsorbent to effectively remove Cd2+ from aqueous solution. The characteristics were analysed by SEM-EDS, XRD, BET, FTIR and XPS. Different factors that affected the Cd2+ removal on MRM, such as dosage, initial pH, initial Cd2+ concentration, were investigated using batch adsorption experiments. Simultaneously, the adsorption kinetics, adsorption isotherms and adsorption thermodynamics of Cd2+ were also investigated using adsorption experiments data. The characterization results showed that MRM had a rougher, larger specific surface area and pore volume (38.91 m2 g-1, 0.02 cm3 g-1) than RM (10.22 m2 g-1, 0.73 cm3 g-1). The adsorption experiments found that the equilibrium adsorption capacity of MRM for Cd2+ was significantly increased to 46.36 mg g-1, which was almost three times that of RM. According to the fitting results, the pseudo-second-order kinetic model described the adsorption process better than the pseudo-first-order kinetic model. The Langmuir model fitted the adsorption isotherms well, indicating that the adsorption process was unimolecular layer adsorption and the maximum capacity was 103.59 mg g-1. The thermodynamic parameters indicated that the adsorption process was heat-trapping and spontaneous. Finally, combined XPS and FTIR studies, it was speculated that the adsorption mechanisms should be electrostatic attachment, specific adsorption (i.e., Cd-O or hydroxyl binding) and ion exchange. Therefore, manganese dioxide modified red mud can be an effective and economical alternative to the removal of Cd2+ in the wastewater treatment process.


Assuntos
Cádmio , Poluentes Químicos da Água , Adsorção , Cádmio/análise , Concentração de Íons de Hidrogênio , Cinética , Compostos de Manganês , Óxidos , Termodinâmica , Água/química , Poluentes Químicos da Água/análise
9.
Biosensors (Basel) ; 12(3)2022 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-35323438

RESUMO

Respiration rate is an essential vital sign that requires monitoring under various conditions, including in strong electromagnetic environments such as in magnetic resonance imaging systems. To provide an electromagnetically-immune breath-sensing system, we propose an all-fiber-optic wearable breath sensor based on a fiber-tip microcantilever. The microcantilever was fabricated on a fiber-tip by two-photon polymerization microfabrication based on femtosecond laser, so that a micro Fabry-Pérot (FP) interferometer was formed between the microcantilever and the end-face of the fiber. The cavity length of the micro FP interferometer was reduced as a result of the bending of the microcantilever induced by breath airflow. The signal of breath rate was rebuilt by detecting power variations of the FP interferometer reflected light and applying dynamic thresholds. The breath sensor achieved a high sensitivity of 0.8 nm/(m/s) by detecting the reflection spectrum upon applied flow velocities from 0.53 to 5.31 m/s. This sensor was also shown to have excellent thermal stability as its cross-sensitivity of airflow with respect to the temperature response was only 0.095 (m/s)/°C. When mounted inside a wearable surgical mask, the sensor demonstrated the capability to detect various breath patterns, including normal, fast, random, and deep breaths. We anticipate the proposed wearable breath sensor could be a useful and reliable tool for respiration rate monitoring.


Assuntos
Tecnologia de Fibra Óptica , Dispositivos Eletrônicos Vestíveis , Lasers , Fibras Ópticas , Temperatura
10.
J Asian Nat Prod Res ; : 1-7, 2022 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-35352584

RESUMO

Two new halogenated metabolites, laurenhalogens A (1) and B (2), along with four known ones (3-6), were isolated from the red alga Laurencia sp. The structures of 1 and 2 were determined by the means of UV, IR, MS, NMR and X-ray diffraction analysis. In addition, the antibacterial activities of 1-6 were also evaluated.

11.
Oncol Rep ; 47(5)2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35315503

RESUMO

Following the publication of the above article, an interested reader drew the authors' attention to the fact that certain features shown in Fig. 6B, illustrating the tumors extracted from the animal in vivo experiments, were strikingly similar to images that had appeared in other papers by different authors published at around the same time. The authors conceded that the in vivo experiments reported in this study had been performed by a third party. Therefore, in the interests of preserving accuracy in the scientific record, the authors requested that this paper be retracted from the Journal. The Editor is in agreement that the paper should be retracted. All authors agree with the retraction of this article, and the Editor apologizes to the readership for any inconvenience caused. [the original article was published in Oncology Reports 45: 1094­1104, 2021; DOI: 10.3892/or.2020.7908].

12.
Macromol Rapid Commun ; : e2200017, 2022 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-35150177

RESUMO

The photochemical reaction is a very important type of chemical reaction. Visualizing and controlling photo-mediated reactions is a long-standing goal and challenge. In this regard, single-molecule electrical detection with label-free, real-time, and in situ characteristics has unique advantages in monitoring the dynamic process of photoreactions at the single-molecule level. In this review, a valuable summary of the latest process of single-molecule photochemical reactions based on single-molecule electrical platforms is provided. The single-molecule electrical detection platforms for monitoring photoreactions are displayed, including their fundamental principles, construction methods, and practical applications. The single-molecule studies of two different types of light-mediated reactions are summarized as below: i) photo-induced reactions, including reversible cyclization, conformational isomerization, and other photo-related reactions; ii) plasmon-mediated photoreactions, including reaction mechanisms and concrete examples, such as plasmon-induced photolysis of SS/OO bonds and tautomerization of porphycene. In addition, the prospects for future research directions and challenges in this field are also discussed.

13.
Stem Cell Res Ther ; 13(1): 9, 2022 01 10.
Artigo em Inglês | MEDLINE | ID: mdl-35012650

RESUMO

Coronavirus disease 2019 (COVID-19) caused by the novel severe acute respiratory coronavirus 2 is currently spreading throughout the world with a high rate of infection and mortality and poses a huge threat to global public health. COVID-19 primarily manifests as hypoxic respiratory failure and acute respiratory distress syndrome, which can lead to multiple organ failure. Despite advances in the supportive care approaches, there is still a lack of clinically effective therapies, and there is an urgent need to develop novel strategies to fight this disease. Currently, stem cell therapy and stem cell-derived organoid models have received extensive attention as a new treatment and research method for COVID-19. Here, we discuss how stem cells play a role in the battle against COVID-19 and present a systematic review and prospective of the study on stem cell treatment and organoid models of COVID-19, which provides a reference for the effective control of the COVID-19 pandemic worldwide.


Assuntos
COVID-19 , Pandemias , Humanos , Estudos Prospectivos , SARS-CoV-2 , Células-Tronco
14.
Oncol Rep ; 47(3)2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34981820

RESUMO

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the representative tumor images shown in Fig. 6B were strikingly similar to images appearing in different form in another article by different authors. Subsequently to the comments raised by the reader, the authors themselves contacted the Editorial Office to request a retraction of the above paper on account of not being to reproduce the results shown in the in vivo experiments. Owing to the fact that the contentious data in the above article had already been published elsewhere prior to its submission to Oncology Reports, and in line with the authors' own request, the Editor has decided that this paper should be retracted from the Journal. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in Oncology Reports 39: 1173­1180, 2018; DOI: 10.3892/or.2017.6157].

15.
BMC Complement Med Ther ; 22(1): 25, 2022 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-35086536

RESUMO

BACKGROUND: Osteoarthritis (OA) treatment aims to improve inflammation and delay cartilage degeneration. However, there is no effective strategy presently available. Ononin, a representative isoflavone glycoside component extracted from natural Chinese herbs, exerts anti-inflammatory and proliferative effects. However, the therapeutic effect of ononin on chondrocyte inflammation remains unclear. METHODS: In this study, we explored the therapeutic effect and potential mechanism of ononin in OA by establishing an interleukin-1 beta (IL-1ß)-induced chondrocyte inflammation model. RESULTS: Our results verified that ononin alleviated the IL-1ß-induced decrease in chondrocyte viability, attenuated the overexpression of the inflammatory factors tumour necrosis factor α (TNF-α) and interleukin 6 (IL-6), and simultaneously inhibited the expression of cartilage extracellular matrix (ECM)-degrading enzymes such as matrix metalloproteinase-13 (MMP-13). Furthermore, the decomposition of Collagen II protein could be alleviated in the OA model by ononin. Finally, ononin improved chondrocyte inflammation by downregulating the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) signalling pathways. CONCLUSION: Our findings suggested that ononin could inhibit the IL-1ß-induced proinflammatory response and ECM degradation in chondrocytes by interfering with the abnormal activation of the MAPK and NF-κB pathways, indicating its protective effect against OA.


Assuntos
Cartilagem/efeitos dos fármacos , Glucosídeos/farmacologia , Inflamação/metabolismo , Interleucina-1beta/metabolismo , Isoflavonas/farmacologia , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/metabolismo , Osteoartrite , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Cartilagem/citologia , Cartilagem/metabolismo , Cartilagem/patologia , Condrócitos/efeitos dos fármacos , Condrócitos/metabolismo , Condrócitos/patologia , Regulação para Baixo , Glucosídeos/uso terapêutico , Inflamação/tratamento farmacológico , Isoflavonas/uso terapêutico , Sistema de Sinalização das MAP Quinases , Masculino , Metaloproteinase 13 da Matriz/metabolismo , Osteoartrite/tratamento farmacológico , Osteoartrite/metabolismo , Osteoartrite/patologia , Fitoterapia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Ratos Sprague-Dawley , Transdução de Sinais , Fator de Necrose Tumoral alfa/metabolismo
16.
Int J Biol Macromol ; 199: 189-200, 2022 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-34973981

RESUMO

The inhibition of human islet amyloid polypeptide (hIAPP) deposition to block its toxicity is an important strategy for the prevention and treatment of type II diabetes mellitus (T2DM).Natural compounds with pharmacological properties and low toxicity can serve as a good point to discover potential inhibitors of protein misfolding, which may be useful for the treatment of various amyloidosis-related diseases. Previous studies have reported that triterpenoids, such as maslinic acid (MA) and momordicin I (MI), can modulate glucose metabolism partially by reducing insulin resistance. However, the internal antidiabetic mechanism of these triterpenoids remains unclear. In this study, we examined the inhibition and disaggregation of MAandits isomer MI on the fibrillation of hIAPP using various experimental and computational approaches. The assembly behaviors and peptide-induced cytotoxicity of hIAPP could be effectively resisted by MA and MI. Moreover, the interaction of the two triterpenoids with hIAPP displayed a spontaneous and exothermic process. Moreover, molecular dynamics simulation results of different peptides revealed that MA and MI could bind to Asn and other non-polar residues near the core C-terminal region and reduce the oligomerization of hIAPP. The binding affinity was predominantly contributed by hydrophobic, electrostatic and hydrogen bonding interactions. The present work provides valuable data for MA and MI to treat T2DM and amyloidosis-related diseases.


Assuntos
Amiloidose , Diabetes Mellitus Tipo 2 , Triterpenos , Amiloide/metabolismo , Amiloidose/tratamento farmacológico , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/metabolismo , Humanos , Polipeptídeo Amiloide das Ilhotas Pancreáticas/metabolismo , Simulação de Dinâmica Molecular , Triterpenos/farmacologia , Triterpenos/uso terapêutico
17.
Int Urol Nephrol ; 54(4): 959-967, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34383207

RESUMO

PURPOSE: To analyze the efficacy of immunosuppressive therapy in IgA nephropathy and investigate the value of all clinicopathologic indicators. METHOD: One hundred and one eligible IgA nephropathy patients were retrospectively studied. All the patients received immunosuppressive treatment and were then grouped according to the treatment outcome. The endpoint was a composite outcome (halving eGFR, end-stage renal disease (ESRD) or death due to kidney disease). The outcomes of immunosuppressive therapy were evaluated, and the factors influencing the outcomes of immunosuppressive therapy were analyzed by logistics regression. The independent significance of clinicopathologic indicators on renal outcome was then analyzed by multivariable Cox regression. RESULTS: Multivariate logistic regression analysis showed that S1 and M1 were the risk factors for the immunosuppressive treatment effect in IgAN patients, and eGFR was the protective factor for the immunosuppressive treatment effect in IgAN patients. Kaplan-Meier analysis revealed that outcomes of immunosuppressive therapy were significantly associated with poor renal outcomes. Multiple Cox regression analysis further confirmed that M1, T2, and the initial level of eGFR were independent predictive factors for poor renal outcomes. CONCLUSIONS: M, S scores and initial eGFR are independent predictors of outcomes of immunosuppressive therapy. Only M, T scores can effectively predict poor renal outcomes after immunosuppressive therapy. Nonetheless, stable eGFR and low proteinuria can protect renal outcomes.


Assuntos
Glomerulonefrite por IGA , Progressão da Doença , Taxa de Filtração Glomerular , Glomerulonefrite por IGA/complicações , Glomerulonefrite por IGA/tratamento farmacológico , Glomerulonefrite por IGA/patologia , Humanos , Estudos Retrospectivos
18.
Front Plant Sci ; 13: 840614, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35371186

RESUMO

Biofortification is a sustainable strategy to alleviate micronutrient deficiency in humans. It is necessary to improve grain zinc (GZnC) and iron concentrations (GFeC) in wheat based on genetic knowledge. However, the precise dissection of the genetic architecture underlying GZnC and GFeC remains challenging. In this study, high-resolution genome-wide association studies were conducted for GZnC and GFeC by three different models using 166 wheat cultivars and 373,106 polymorphic markers from the wheat 660K and 90K single nucleotide polymorphism (SNP) arrays. Totally, 25 and 16 stable loci were detected for GZnC and GFeC, respectively. Among them, 17 loci for GZnC and 8 for GFeC are likely to be new quantitative trait locus/loci (QTL). Based on gene annotations and expression profiles, 28 promising candidate genes were identified for Zn/Fe uptake (8), transport (11), storage (3), and regulations (6). Of them, 11 genes were putative wheat orthologs of known Arabidopsis and rice genes related to Zn/Fe homeostasis. A brief model, such as genes related to Zn/Fe homeostasis from root uptake, xylem transport to the final seed storage was proposed in wheat. Kompetitive allele-specific PCR (KASP) markers were successfully developed for two major QTL of GZnC on chromosome arms 3AL and 7AL, respectively, which were independent of thousand kernel weight and plant height. The 3AL QTL was further validated in a bi-parental population under multi-environments. A wheat multidrug and toxic compound extrusion (MATE) transporter TraesCS3A01G499300, the ortholog of rice gene OsPEZ2, was identified as a potential candidate gene. This study has advanced our knowledge of the genetic basis underlying GZnC and GFeC in wheat and provides valuable markers and candidate genes for wheat biofortification.

19.
Front Immunol ; 12: 763791, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34880862

RESUMO

Ovarian cancer (OC) is a devastating malignancy with a poor prognosis. The complex tumor immune microenvironment results in only a small number of patients benefiting from immunotherapy. To explore the different factors that lead to immune invasion and determine prognosis and response to immune checkpoint inhibitors (ICIs), we established a prognostic risk scoring model (PRSM) with differential expression of immune-related genes (IRGs) to identify key prognostic IRGs. Patients were divided into high-risk and low-risk groups according to their immune and stromal scores. We used a bioinformatics method to identify four key IRGs that had differences in expression between the two groups and affected prognosis. We evaluated the sensitivity of treatment from three aspects, namely chemotherapy, targeted inhibitors (TIs), and immunotherapy, to evaluate the value of prediction models and key prognostic IRGs in the clinical treatment of OC. Univariate and multivariate Cox regression analyses revealed that these four key IRGs were independent prognostic factors of overall survival in OC patients. In the high-risk group comprising four genes, macrophage M0 cells, macrophage M2 cells, and regulatory T cells, observed to be associated with poor overall survival in our study, were higher. The high-risk group had a high immunophenoscore, indicating a better response to ICIs. Taken together, we constructed a PRSM and identified four key prognostic IRGs for predicting survival and response to ICIs. Finally, the expression of these key genes in OC was evaluated using RT-qPCR. Thus, these genes provide a novel predictive biomarker for immunotherapy and immunomodulation.


Assuntos
Neoplasias Ovarianas/imunologia , Biologia Computacional , Feminino , Humanos , Inibidores de Checkpoint Imunológico/uso terapêutico , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Ovarianas/genética , Prognóstico , Modelos de Riscos Proporcionais
20.
J Phys Chem Lett ; 12(51): 12352-12359, 2021 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-34935382

RESUMO

Antimony selenide (Sb2Se3) has attracted considerable attention for its simple composition, nontoxic nature, and abundance. However, the efficiency of Sb2Se3 solar cells is limited by the low carrier concentration and high recombination rate at the interface between Sb2Se3 and the Au layer. For this paper, the KOH solution used as the etchant was used to increase the efficiency of Sb2Se3 solar cells. The KOH solution not only reacts with (etching the surface) but also diffuses inside the Sb2Se3 film. This study unexpectedly demonstrates that KOH also increases the doping density and improves the back contact (the thin Sb2O3 layer exists with the etching of the KOH solution) of Sb2Se3 solar cells. Lastly, the best power conversion efficiency of 7.16% is demonstrated with a high open-circuit voltage of 0.407 V; we believe our work can serve as a guide for further development of high-efficiency Sb2Se3 solar cells.

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