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1.
Chem Commun (Camb) ; 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36112013

RESUMO

The high gravimetric energy density and environmental benefits place hydrogen as a promising alternative to the widely used fossil fuels, which is however impeded by the lack of safe, energy-saving and cost-effective H2 storage systems. The use of solid adsorbents as candidate materials offers a less energy-intensive way of storing hydrogen. The exceptional diversity and tunability of the chemical composition, topological structure, and surface chemistry together with large surface area position porous metal-organic frameworks as promising hydrogen storage material candidates. In this review, we first introduce several classes of important metal-organic frameworks for hydrogen storage, and then highlight the progress associated with the key challenges to be addressed, including the improvement of hydrogen-framework interaction required for enhancing room-temperature hydrogen storage capacities, and the optimization/balance of both gravimetric and volumetric storage/working capacities. In particular, the strategies used to tune and enhance hydrogen binding energies have been comprehensively reviewed. Future development prospects and related challenges of using porous metal-organic frameworks as hydrogen storage materials are also outlined. This feature review provides a wide perspective and insightful thoughts and suggestions for hydrogen storage using metal-organic frameworks, and promotes the further development of hydrogen storage materials to realize a hydrogen economy.

2.
Inorg Chem ; 61(34): 13627-13636, 2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-35980758

RESUMO

Excessive content of fluoride ions (F-) in water will lead to water pollution and endanger human health, so the research on the method of low-cost, rapid, and efficient detection of F- is of particular significance. In this work, an amino-functionalized ligand with an appropriate triplet energy excited state, 2'-amino-[1,1':4',1″-terphenyl]-3,3″,5,5″-tetracarboxylic acid (H4TPTC-NH2), was selected to construct a luminescent single-lanthanide metal-organic framework, EuTPTC-NH2, with uncoordinated amino groups for the detection of F-. Based on host-guest interactions, that is, hydrogen bonds formed between the free amino groups and F- ions, EuTPTC-NH2 was developed as a ratiometric fluorescence probe for F- detection with good anti-interference ability, low detection limit, high water stability, and selectivity. It was found that EuTPTC-NH2 has an excellent linear response to F- in the concentration range of 0-80 µM with high sensitivity and a low detection limit of 11.26 µM. A hydrogel membrane based on the combination of EuTPTC-NH2 and agarose was also prepared for the quantitative visual detection of F- in water.


Assuntos
Elementos da Série dos Lantanídeos , Estruturas Metalorgânicas , Corantes Fluorescentes , Fluoretos , Flúor , Humanos , Água
3.
Inorg Chem ; 61(12): 5067-5075, 2022 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-35289607

RESUMO

Detection of H2S in the biological system has attracted enormous attention in recent years. In this work, a new vinyl-functionalized metal-organic framework (MOF), [(Me2NH2)2] [Eu6(µ3-OH)8(BDC-CH═CH2)6(H2O)6] (Eu-BDC-CH═CH2, BDC-CH═CH2 = 2-vinylterephthalic acid), was synthesized under solvothermal conditions. The vinyl groups in the ligands can not only modulate the "antenna effect" of the ligand on Eu3+ ions but also serve as an exposed reactive site to allow for the quantitative detection of H2S by Eu-BDC-CH═CH2. The ratiometric fluorescent probe has the advantages of water stability, acid-base stability (pH = 2-11), fast response (<2 min), high selectivity, and sensitivity (LOD = 38.4 µM). We also used Eu-BDC-CH═CH2 to detect and analyze H2S in tap and lake waters, demonstrating the potential of the probe for biological and environmental applications. In addition, the MOF-based agarose hydrogel film allows for the visual detection of H2S via a smartphone by identifying the RGB values. The vinyl-functionalized MOF can thus be a powerful sensing platform for H2S.


Assuntos
Sulfeto de Hidrogênio , Estruturas Metalorgânicas , Corantes Fluorescentes/química , Ligantes , Estruturas Metalorgânicas/química , Água/química
4.
Chem Commun (Camb) ; 58(6): 747-770, 2022 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-34979539

RESUMO

Metal-organic frameworks (MOFs), as an emerging class of porous materials, excel in designability, regulatability, and modifiability in terms of their composition, topology, pore size, and surface chemistry, thus affording a huge potential for addressing environment and energy-related challenges. In particular, MOFs can be applied as porous adsorbents for the purification of industrially important hydrocarbons through certain process-efficient separation schemes based on selectivity-reversed adsorption and multicomponent separation. Moreover, the vast combination possibilities and controllable and engineerable luminescent units of MOFs make them a versatile platform to develop functionally tailored materials for luminescent sensing and optical data encryption. In this feature article, we summarize the recent progress in the use of porous MOFs for the separation and purification of acetylene (C2H2) and ethylene (C2H4) based on selectivity-reversed adsorption and multicomponent separation strategies. Moreover, we highlight the advances over the past three years in the field of MOF-based luminescent materials for thermometry, turn-on sensing, and information encryption.

5.
Environ Microbiol ; 24(1): 98-109, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34913576

RESUMO

Based on 16S rRNA gene analyses, the same bacterial operational taxonomic units (OTUs) are common to both the Arctic and Antarctic oceans, supporting the concept 'everything is everywhere'. However, whether the same OTUs from both poles have identical genomes, i.e. whether 'everything is still everywhere' at the genomic level has not yet been examined systematically. Here, we isolated, sequenced and compared the genomes of 45 culturable marine bacteria belonging to three genera of Salinibacterium, Psychrobacter and Pseudoalteromonas from both polar oceans. The bacterial strains with identical 16S rRNA genes were common to both poles in every genus, and four identical genomes were detected in the genus Salinibacterium from the Arctic region. However, no identical genomes were observed from opposite poles in this study. Our data, therefore, suggest that 'everything is not everywhere' at the genomic level. The divergence time between bacteria is hypothesized to exert a strong impact on the bacterial biogeography at the genomic level. The geographical isolation between poles was observed for recently diverged, highly similar genomes, but not for moderately similar genomes. This study thus improves our understanding of the factors affecting the genomic-level biogeography of marine microorganisms isolated from distant locations.


Assuntos
Genômica , Pseudoalteromonas , Regiões Antárticas , Geografia , Filogenia , Pseudoalteromonas/genética , RNA Ribossômico 16S/genética
6.
Cancer Lett ; 519: 315-327, 2021 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-34343634

RESUMO

Recent studies suggest that RRP15 (Ribosomal RNA Processing 15 Homolog) might be a potential target for cancer therapy. However, the role of RRP15 in hepatocarcinogenesis remains poorly delineated. In this study, we aimed to evaluate the expression and biological function of RRP15 in human hepatocellular carcinoma (HCC). We show that RRP15 was up regulated in HCC cell lines and tumours. Up-regulation of RRP15 in HCC tumours was also correlated with unfavorable prognosis. We further show that the frequent up-regulation of RRP15 in HCCs is at least partly driven by recurrent gene copy gain at chromosome 1q41. Functional studies indicated that RRP15 knockdown suppresses HCC proliferation and growth both in vitro and in vivo. Mechanistically, RRP15 depletion in p53-wild-type HepG2 cells induced senescence via activation of the p53-p21 signalling pathway through enhanced interaction of RPL11 with MDM2, as well as inhibition of SIRT1-mediated p53 deacetylation. Moreover, RRP15 depletion in p53-mutant PLC5 and p53-deleted Hep3B cells induced metabolic shift from the glycolytic pentose-phosphate to mitochondrial oxidative phosphorylation via regulating a series of key genes such as HK2 and TIGAR, and thus, promoted the generation of ROS and apoptosis. Taken together, our findings provide evidence for an important role of the RRP15 gene in hepatocarcinogenesis through regulation of HCC proliferation and growth, raising the possibility that targeting RRP15 may represent a potential therapeutic strategy for HCC treatment.


Assuntos
Apoptose/genética , Carcinoma Hepatocelular/genética , Proliferação de Células/genética , Senescência Celular/genética , Neoplasias Hepáticas/genética , RNA Ribossômico/genética , Proteínas Ribossômicas/genética , Carcinogênese/genética , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Movimento Celular/genética , Regulação Neoplásica da Expressão Gênica/genética , Células Hep G2 , Hexoquinase/genética , Humanos , Neoplasias Hepáticas/patologia , Recidiva Local de Neoplasia/genética , Recidiva Local de Neoplasia/patologia , Monoéster Fosfórico Hidrolases/genética , Transdução de Sinais/genética , Proteína Supressora de Tumor p53/genética
7.
ACS Appl Mater Interfaces ; 13(17): 20371-20379, 2021 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-33885284

RESUMO

Fabrication of dual-emitting materials for H2S sensing under environmental and biological conditions is currently of great interest. In this work, a new chemically stable metal supramolecular complex [Zn2(pda)2(H2O)3]·(H2O)0.5 (Znpda, pda = 1,10-phenanthroline-2,9-dicarboxylic acid), with accessible uncoordinated carboxylic oxygen sites, is solvothermally synthesized. It can serve as a host in luminescent hybrid composites. By incorporating Eu3+ and Cu2+ in the supramolecular coordination network, we obtained the dual-emitting hybrid material Eu3+/Cu2+@Znpda, which simultaneously shows intense ligand and weak Eu3+ emissions in HEPES buffer solution. Since H2S can easily chelate with Cu2+ and recover the blocked "antenna effect" between the ligand and Eu3+, Eu3+/Cu2+@Znpda possesses both the turn-on and ratiomectric fluorescence response to H2S. Accordingly, we designed an IMPLICATION logic gate for H2S recognition by employing the fluorescence intensity ratio between the ligand and Eu3+ as the output signal. In addition, Eu3+/Cu2+@Znpda shows a fast response (<1 min) and high sensitivity (1.45 µM) to H2S over other interfering species in the HEPES buffer solution, highlighting its potential use for H2S sensing under environmental and biological conditions.


Assuntos
Cobre/química , Európio/química , Corantes Fluorescentes/química , Sulfeto de Hidrogênio/análise , Zinco/química , Ligação de Hidrogênio , Luminescência
8.
Inorg Chem ; 60(6): 4133-4143, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33660997

RESUMO

In this study, two substituent-group-modifying tetracarboxylate ligands, 2',5'-dimethoxy-[1,1':4',1″-terphenyl]-3,3″,5,5″-tetracarboxylic acid (H4TPTC-2OMe) and 2',5'-dimethyl-[1,1':4',1″-terphenyl]-3,3″,5,5″-tetracarboxylic acid (H4TPTC-2Me), with similar geometries were used as the organic linkers to construct isostructural lanthanide metal-organic frameworks (LnMOFs). The as-prepared LnTPTC-2OMe and LnTPTC-2Me were structurally elucidated by means of single-crystal and powder X-ray diffraction in addition to thermogravimetric analysis and were assessed as luminescence ratiometric thermometers by obtaining the temperature dependence of the luminescence behaviors. We found that both the single lanthanide EuTPTC-2OMe and the dual lanthanide Eu0.05Tb0.95TPTC-2Me exhibited a distinct S-type luminescence response to temperatures in the range from 313 to 473 K, and their ratiometric parameters can be understood on the basis of the classic Mott-Seitz model. Energy transfers from the ligand to Tb3+ (or Eu3+) and from Tb3+ to Eu3+ in these two systems were investigated theoretically as well as with low-temperature (77 K) time-resolved photoluminescence spectroscopy, quantum yield, and lifetime analysis. Therefore, these two materials possess a good relative sensitivity, a small temperature uncertainty, and a favorable spectral repeatability in addition to a remarkable emission color change, enhancing their potential use for temperature measurement and in situ monitoring in microelectronics.

9.
Chem Commun (Camb) ; 57(25): 3143-3146, 2021 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-33634810

RESUMO

Three different thermo-responsive fluorescent thermometers were constructed by regulating the triplet energy level of organic ligands in isostructural Eu/Tb mixed MOFs. Among them, a quite unusual and rarely reported temperature-dependent fluorescence behavior was observed in LnBDC-NH2, and Eu0.01Tb0.99NDC is effective in the physiological range with the maximum relative sensitivity of 7.32% °C-1.


Assuntos
Európio/química , Limite de Detecção , Estruturas Metalorgânicas/química , Temperatura , Térbio/química , Termometria/instrumentação
10.
ACS Appl Mater Interfaces ; 13(9): 11078-11088, 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33635069

RESUMO

The intrinsic advantages of metal-organic frameworks (MOFs), including extraordinarily high porosities, tailorable architectures, and diverse functional sites, make the MOFs platforms for multifunctional materials. In this study, we synthesized two kinds of isostructural NbO-type Zn2+-based MOFs, where two structurally similar tetracarboxylate ligands, 5,5'-(pyrazine-2,5-diyl)diisophthalic acid (H4PZDDI) and 5,5'-(pyridine-2,5-diyl)diisophthalic acid (H4PDDI), with pyridine or pyrazine moieties, were employed as the organic linkers. By embedding the red-emitting cationic units of pyridinium hemicyanine dye 4-[p-(dimethylamino)styryl]-1-methylpyridinium (DSM) and trivalent europium ion (Eu3+), two types of composites, DSM@ZnPZDDI and DSM@ZJU-56 and Eu3+@ZnPZDDI and Eu3+@ZJU-56, were harvested and evaluated for use as potential ratiometric temperature probes. The temperature-responsive luminescence of these dual-emitting composites was investigated, and their representative features of relative sensitivity, temperature resolution, spectral repeatability, and luminescence color change were discussed. Importantly, compared with the DSM-incorporated composites, Eu3+@ZnPZDDI and Eu3+@ZJU-56 show a much wider sensing temperature range and higher relative sensitivities, suggesting the performance of the composites can be engineered by elaborately combining the host and guest units. Given the rich choices of porous MOFs and emitting units, such a strategy can be useful in the design and preparation of multifunctional dual-emitting sensory materials.

11.
Dis Esophagus ; 34(10)2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-33442745

RESUMO

Endoscopic submucosal dissection (ESD) is widely used for early stage esophageal cancer and precancerous lesions. Non-cardiac chest pain (NCCP) is a frequent complication of ESD. However, little is known about its incidence and associated factors. This study investigated the pain incidence and predictive factors for pain development after ESD for esophageal neoplasms. We enrolled a total of 309 patients with esophageal neoplasms, who underwent ESD in our center from January 2018 to June 2019. Sociodemographic and clinicopathological information for all patients was collected, and patients were divided into either a pain-free group (n = 156) or a pain group (n = 153) according to whether there was onset of NCCP 24-48 hours after surgery. We made comparisons between groups using Student's t test or the χ2 test. Logistic-regression analysis was used to screen for risk factors. There were statistically significant differences in histories of previous surgery (P = 0.039), lesion size (P = 0.026), operation time (P = 0.009), and postoperative fever (P = 0.001). History of previous surgery (P = 0.043) and postoperative fever (P = 0.007) were independent risk factors for chest pain after esophageal ESD treatment. Chest pain and fever prolonged postoperative hospitalization time (P = 0.005, P = 0.001) and increased hospitalization cost (P = 0.034, P < 0.001). A history of previous surgery and postoperative fever was associated with the occurrence of NCCP after ESD in patients with esophageal neoplasms. NCCP and fever after esophageal ESD increased both hospitalization time and cost.


Assuntos
Ressecção Endoscópica de Mucosa , Neoplasias Esofágicas , Estudos de Casos e Controles , Dor no Peito/epidemiologia , Dor no Peito/etiologia , Ressecção Endoscópica de Mucosa/efeitos adversos , Neoplasias Esofágicas/cirurgia , Humanos , Estudos Retrospectivos , Resultado do Tratamento
12.
Nat Prod Res ; 35(18): 3171-3175, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31741408

RESUMO

Daphne giraldii Nitsche, belongs to Daphne genus, has been reported to exert anti-tumor activities. Our previous study suggested that flavones from Daphne giraldii have significant inhibitory effects on hepatocellular carcinoma (HCC) cells. However, the potential target of this type flavone was still unknown. In this study, 74 flavonoids compounds of Daphne giraldii and 41 potential targets of HCC were analyzed by the network, the most potential target was histone deacetylase 6 (HDAC6). Considering the cytotoxicity, compound 70 (Daphnegiravone D, DGD) was chosen for further confirmation. Molecular docking study revealed that DGD formed high binding affinity with HDAC6. Concomitantly, pharmacological studies indicated that DGD could inhibit the expression of HDAC6 in vitro and in vivo. In this study, network pharmacology along with experimental validation predicted and verified HDAC6 as one of potential targets of flavones, these investigations provide a new insight for further study of Daphne giraldii on HCC treatment.


Assuntos
Carcinoma Hepatocelular , Daphne , Flavonas , Desacetilase 6 de Histona/antagonistas & inibidores , Neoplasias Hepáticas , Carcinoma Hepatocelular/tratamento farmacológico , Linhagem Celular Tumoral , Daphne/química , Flavonas/isolamento & purificação , Flavonas/farmacologia , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Simulação de Acoplamento Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
13.
Evol Appl ; 13(10): 2821-2835, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33294025

RESUMO

Local adaptation is particularly likely in invertebrate pests that typically have short generation times and large population sizes, but there are few studies on pest species investigating local adaptation and separating this process from contemporaneous and historical gene flow. Here, we use a population genomic approach to investigate evolutionary processes in the two most dominant spider mites in China, Tetranychus truncatus Ehara and Tetranychus pueraricola Ehara et Gotoh, which have wide distributions, short generation times, and large population sizes. We generated genome resequencing of 246 spider mites mostly from China, as well as Japan and Canada at a combined total depth of 3,133×. Based on demographic reconstruction, we found that both mite species likely originated from refugia in southwestern China and then spread to other regions, with the dominant T. truncatus spreading ~3,000 years later than T. pueraricola. Estimated changes in population sizes of the pests matched known periods of glaciation and reinforce the recent expansion of the dominant spider mites. T. truncatus showed a greater extent of local adaptation with more genes (76 vs. 17) associated with precipitation, including candidates involved in regulation of homeostasis of water and ions, signal transduction, and motor skills. In both species, many genes (135 in T. truncatus and 95 in T. pueraricola) also showed signatures of selection related to elevation, including G-protein-coupled receptors, cytochrome P450s, and ABC-transporters. Our results point to historical expansion processes and climatic adaptation in these pests which could have contributed to their growing importance, particularly in the case of T. truncatus.

14.
Insect Sci ; 27(5): 947-963, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32198842

RESUMO

Rice planthoppers are notorious plant sap-feeding pests which cause serious damage. While several microbes in rice planthoppers have been broadly characterized, the abundance and diversity of bacteria and fungi in field planthoppers are largely unknown. This study investigated the bacterial and fungal community compositions of Chinese wild rice planthoppers Laodelphax striatellus and Sogatella furcifera using parallel 16S rRNA gene amplicon and internal transcribed space region sequencing. The bacteria varied significantly between the species and were partitioned significantly by sex, tissues and host environments in each species. The majority of bacteria were affiliated with the genera Wolbachia, Cardinium, Rickettsia and Pantoea. The abundance of Wolbachia was negatively correlated with that of Cardinium in both planthopper species. Compared with bacteria, the abundance and diversity of fungi did not differ between sexes but both were enriched in the gut. The bacterial community as a whole showed no significant correlation with the fungal community. The majority of fungi were related to Sarocladium, Alternaria, Malassezia, Aspergillus and Curvularia. A phylogenetic analysis revealed that these fungi were closely related to botanic symbionts or pathogens. Our results provide novel insights into the bacteria and fungi of rice planthoppers.


Assuntos
Bactérias/isolamento & purificação , Fungos/isolamento & purificação , Hemípteros/microbiologia , Microbiota , Animais , Bactérias/classificação , China , Meio Ambiente , Feminino , Fungos/classificação , Masculino , Micobioma , Filogenia , RNA Bacteriano/análise , RNA Fúngico/análise , RNA Ribossômico 16S/análise , Fatores Sexuais , Especificidade da Espécie , Distribuição Tecidual
15.
Genome Biol Evol ; 12(2): 3818-3831, 2020 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-31958110

RESUMO

Wolbachia is a widely distributed intracellular bacterial endosymbiont among invertebrates. The wStriCN, the Wolbachia strain that naturally infects an agricultural pest Laodelphax striatellus, has a "Jekyll and Hyde" mode of infection pattern with positive and negative effects: It not only kills many offspring by inducing cytoplasmic incompatibility (CI) but also significantly increases host fecundity. In this study, we assembled the draft genome of wStriCN and compared it with other Wolbachia genomes to look for clues to its Jekyll and Hyde characteristics. The assembled wStriCN draft genome is 1.79 Mb in size, which is the largest Wolbachia genome in supergroup B. Phylogenomic analysis showed that wStriCN is closest to Wolbachia from Asian citrus psyllid Diaphorina citri. These strains formed a monophylogentic clade within supergroup B. Compared with other Wolbachia genomes, wStriCN contains the most diverse insertion sequence families, the largest amount of prophage sequences, and the most ankyrin domain protein coding genes. The wStriCN genome encodes components of multiple secretion systems, including Types I, II, IV, VI, Sec, and Tac. We detected three pairs of homologs for CI factors CifA and CifB. These proteins harbor the catalytic domains responsible for CI phenotypes but are phylogenetically and structurally distinct from all known Cif proteins. The genome retains pathways for synthesizing biotin and riboflavin, which may explain the beneficial roles of wStriCN in its host planthoppers, which feed on nutrient-poor plant sap. Altogether, the genomic sequencing of wStriCN provides insight into understanding the phylogeny and biology of Wolbachia.


Assuntos
Hemípteros/microbiologia , Wolbachia/genética , Animais , Proteínas de Bactérias/genética , Biotina/metabolismo , Genoma Bacteriano/genética , Genômica/métodos , Fenótipo , Filogenia , Riboflavina/metabolismo
16.
Insect Sci ; 27(5): 895-907, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30924288

RESUMO

Bacteria symbionts in herbivores play an important role in host biology and ecology, and are affected by environmental factors such as temperature, diet, habitat, antibiotics and so on. However, the effects of antibiotics on the microbiome of the small brown planthopper Laodelphax striatellus (SBPH) remain unclear. Here, we studied the effects of tetracycline on the diversity and composition of bacterial colonies in different tissues of SBPH using high throughput sequencing of 16S ribosomal RNA amplicons. Our results show that Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria were most abundant in SBPH, and the genera Asaia and Wolbachia were most abundant in all body parts of SBPH. Antibiotic treatment had persistent effects on the composition of the SBPH microbiome. Tetracycline depleted the population of Firmicutes, Bacteroidetes, Tenericutes and Fusobacteria, and nearly 100% eliminated Wolbachia, Bacteroides and Abiotrophia in SBPH. Together, these results suggest that antibiotic exposure affects the bacteria symbionts of different body parts in SBPH and will facilitate future studies of the bacterial symbionts of arthropod hosts.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Hemípteros/microbiologia , Microbiota/efeitos dos fármacos , Tetraciclina/farmacologia , Animais , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , RNA Bacteriano/análise , RNA Ribossômico 16S/análise , Distribuição Tecidual
17.
ISME J ; 14(3): 676-687, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31767943

RESUMO

Symbiont-mediated nutritional mutualisms can contribute to the host fitness of insects, especially for those that feed exclusively on nutritionally unbalanced diets. Here, we elucidate the importance of B group vitamins in the association of endosymbiotic bacteria Wolbachia with two plant-sap feeding insects, the small brown planthopper, Laodelphax striatellus (Fallén), and the brown planthopper, Nilaparvata lugens (Stål). Infected planthoppers of both species laid more eggs than uninfected planthoppers, while the experimental transfer of Wolbachia into uninfected lines of one planthopper species rescued this fecundity deficit. The genomic analysis showed that Wolbachia strains from the two planthopper species encoded complete biosynthesis operons for biotin and riboflavin, while a metabolic analysis revealed that Wolbachia-infected planthoppers of both species had higher titers of biotin and riboflavin. Furthermore, experimental supplementation of food with a mixture of biotin and riboflavin recovered the fecundity deficit of Wolbachia-uninfected planthoppers. In addition, comparative genomic analysis suggested that the riboflavin synthesis genes are conserved among Wolbachia supergroups. Biotin operons are rare in Wolbachia, and those described share a recent ancestor that may have been horizontally transferred from Cardinium bacteria. Our research demonstrates a type of mutualism that involves a facultative interaction between Wolbachia and plant-sap feeding insects involving vitamin Bs.


Assuntos
Biotina/metabolismo , Hemípteros/microbiologia , Hemípteros/fisiologia , Riboflavina/metabolismo , Wolbachia/metabolismo , Animais , Fertilidade , Genômica , Reprodução , Simbiose , Complexo Vitamínico B/metabolismo , Wolbachia/genética
18.
Chemistry ; 26(14): 3145-3151, 2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-31886920

RESUMO

Ongoing demand for accurate self-calibrated noninvasive thermometers for micro-/nano-scale applications, particular biomedical diagnosis, is driving the development of temperature sensors. Here a new type of lanthanide metal-organic framework having near-infrared absorption and near-infrared emission features is presented, and it is based on efficient Nd3+ -to-Yb3+ energy transfer in 808 nm photoexcitation. The results show that the ratiometric parameter of Nd0.5 Yb0.5 TPTC (TPTC= 1,1':4',1''-terphenyl]-3,3'',5,5''-tetracarboxylic acid) can deliver good exponential-type luminescence response to temperature in the physiological regime (293-328 K) with high relative sensitivity and accurate temperature resolution, as well as good biocompatibility and chemical stability. Such lanthanide-based materials are especially useful in biomedical applications.


Assuntos
Complexos de Coordenação/química , Corantes Fluorescentes/química , Estruturas Metalorgânicas/química , Neodímio/química , Itérbio/química , Transferência Ressonante de Energia de Fluorescência , Raios Infravermelhos , Ligantes , Estrutura Molecular , Temperatura , Termômetros
19.
Front Mol Biosci ; 7: 621324, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33768113

RESUMO

Atherosclerosis (AS), a kind of chronic inflammatory blood vessel disease, is a main cause of cardiovascular disease, which is a leading cause of mortality around the world. Accumulation of macrophages induced by inflammation contributes to AS development. It has been indicated that microRNAs (miRNAs) are involved in the process of AS. However, the pathway and gene miRNAs targeting are poorly understood. Here we reported that miR-520a-3p was increased in mice with AS and silencing of miR-520a-3p attenuated AS process. Furthermore, inhibition of miR-520a-3p increased the expression of α-SMA and collagen. In addition, miR-520a-3p silencing inhibited the expression of M1 macrophage polarization markers and pro-inflammatory genes and promoted the M2 macrophage polarization. What's more, forced expression of miR-520a-3p diminished IL4/IL13 induced macrophage autophagy via targeting UVRAG. Collectively, our study reveals the role of miR-520a-3p in macrophage polarization and suggests the potential of miRNA as a novel treatment target of AS.

20.
Insect Sci ; 27(5): 859-868, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31411007

RESUMO

Microorganisms provide many physiological functions to herbivorous hosts. Spider mites (genus Tetranychus) are important agricultural pests throughout the world; however, the composition of the spider mite microbial community, especially gut microbiome, remains unclear. Here, we investigated the bacterial community in five spider mite species and their associated feces by deep sequencing of the 16S rRNA gene. The composition of the bacterial community was significantly different among the five prevalent spider mite species, and some bacterial symbionts showed host-species specificity. Moreover, the abundance of the bacterial community in spider mite feces was significantly higher than that in the corresponding spider mite samples. However, Flavobacterium was detected in all samples, and represent a "core microbiome". Remarkably, the maternally inherited endosymbiont Wolbachia was detected in both spider mite and feces. Overall, these results offer insight into the complex community of symbionts in spider mites, and give a new direction for future studies.


Assuntos
Bactérias/isolamento & purificação , Interações entre Hospedeiro e Microrganismos , Microbiota/fisiologia , Simbiose , Tetranychidae/microbiologia , Animais , Fezes/microbiologia , Microbioma Gastrointestinal , RNA Bacteriano/análise , RNA Ribossômico 16S/análise
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