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1.
Clin Exp Rheumatol ; 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31820727

RESUMO

OBJECTIVES: Aneurysm formation can cause life-threatening complications in Takayasu's arteritis (TAK). The objective of this study was to evaluate the demographic, clinical and angiographic features, and outcomes of aneurysm secondary to TAK in Chinese patients. METHODS: The medical charts of patients diagnosed with TAK in Changhai Hospital between 2001 and 2017 were retrospectively reviewed. RESULTS: Aneurysms were identified in 66 (16.6%) of 397 patients with TAK. The mean age at onset was 30.4±11.5 years, with a male:female ratio of 1:2.7. Patients with aneurysm had a higher proportion of male (p<0.01), higher incidences of bruit, chest tightness and aortic regurgitation (all p<0.001), and a lower incidence of visual disturbances (p<0.01) as compared with patients without aneurysm. The prevalence of elevated ESR and CRP and ITAS2010 score were higher in patients with than without aneurysm (all p<0.01). Angiographic classification showed that type V (30.3%) was the most frequent pattern in patients with aneurysm though Type I was dominant in patients without aneurysm. Multiple aneurysms were found in 30.3% of patients and the most common site of aneurysms was abdominal aorta (22.1%). Glucocorticoids were prescribed in 86.4% of patients with aneurysm, and surgical procedures were performed in 80.3%. Five of 52 patients died during the median 3-year follow-up period. CONCLUSIONS: These findings could provide useful information on the demographical, clinical and angiographic features of TAK patients with aneurysm. Aneurysm formation in TAK may be associated with male gender and active vascular inflammation.

2.
J Pharmacol Exp Ther ; 368(2): 218-228, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30530730

RESUMO

Dihydromyricetin (DMY), the main flavonoid of Ampelopsis grossedentata, has potent anti-inflammatory activity. However, the effect of DMY on chronic autoimmune arthritis remains undefined. In this study, we investigated the therapeutic effects of DMY on collagen-induced arthritis (CIA). Wistar rats were immunized with bovine type II collagen to establish CIA and were then administered DMY intraperitoneally (5, 25, and 50 mg/kg) every other day for 5 weeks. Paw swelling, clinical scoring, and histologic analysis were assessed to determine the therapeutic effects of DMY on the development of arthritis in CIA rats. The results showed that treatment with DMY significantly reduced erythema and swelling in the paws of CIA rats. Pathologic analysis of the knee joints and peripheral blood cytokine assay results confirmed the antiarthritic effects of DMY on synovitis and inflammation. Fibroblast-like synoviocytes (FLSs) were isolated from the synovium of CIA rats and treated with 10 ng/ml interleukin (IL)-1ß DMY significantly inhibited the proliferation, migration, and inflammation of IL-1ß-induced FLSs, whereas it significantly increased IL-1ß-induced FLS apoptosis in a dose-dependent manner (6.25-25 µM). Moreover, DMY suppressed phosphorylation of IκB kinase (IKK) and inhibitor of NF-κB α and subsequently reduced the IL-1ß-induced nucleus translocation of NF-κB in FLSs. Through a molecular docking assay, we demonstrated that DMY could directly bind to the Thr9 and Asp88 residues in IKKα and the Asp95, Asn142, and Gln167 residues in IKKß These findings demonstrate that DMY could alleviate inflammation in CIA rats and attenuate IL-1ß-induced activities in FLSs through suppression of NF-κB signaling.


Assuntos
Artrite Experimental/metabolismo , Fibroblastos/efeitos dos fármacos , Flavonóis/uso terapêutico , NF-kappa B/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos , Sinoviócitos/efeitos dos fármacos , Animais , Artrite Experimental/tratamento farmacológico , Artrite Experimental/patologia , Células Cultivadas , Fibroblastos/metabolismo , Flavonóis/farmacologia , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Inflamação/patologia , Masculino , NF-kappa B/metabolismo , Ratos , Ratos Wistar , Transdução de Sinais/fisiologia , Sinoviócitos/metabolismo
3.
CNS Neurosci Ther ; 24(11): 1073-1083, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30277663

RESUMO

AIMS: This study assessed whether antidepressant drug treatment has a common effect on gray matter (GM) volume in MDD patients with and without childhood maltreatment (CM). METHODS: T1-weighted structural magnetic resonance imaging data were collected from 168 participants, including 51 MDD patients with CM, 31 MDD patients without CM, 48 normal controls with CM, and 38 normal controls without CM. MDD patients received 6 months of treatment with paroxetine, and 24 patients with CM, and 16 patients without CM received a second MRI scan. A whole-brain voxel-based morphometry approach was used to estimate GM volume in each participant at two time points. Two-way analysis of variance (ANOVA) was used to determine the effects of MDD and CM on GM volume at baseline. Repeated measures two-way ANOVA was used to determine the treatment-by-CM interactive effect and main effect of treatment during paroxetine treatment. We further investigated the relationship between GM volume and clinical variables. RESULTS: At baseline, significant MDD-by-CM interactive effects on GM volume were mainly observed in the left parahippocampal gyrus, left entorhinal cortex, and left cuneus. GM volume was significantly lower mainly in the right middle temporal gyrus in patients with MDD than in normal controls. We did not find any significant treatment-by-CM interactive effects. However, a treatment-related increase in GM was found in the right middle temporal gyrus in both MDD groups. CONCLUSIONS: These results suggest that paroxetine treatment operates via a shared neurobiological mechanism in MDD patients with and without CM.


Assuntos
Antidepressivos de Segunda Geração/uso terapêutico , Maus-Tratos Infantis/psicologia , Transtorno Depressivo Maior/tratamento farmacológico , Transtorno Depressivo Maior/psicologia , Substância Cinzenta/efeitos dos fármacos , Paroxetina/uso terapêutico , Adulto , Criança , Transtorno Depressivo Maior/diagnóstico por imagem , Feminino , Substância Cinzenta/diagnóstico por imagem , Humanos , Processamento de Imagem Assistida por Computador , Imagem por Ressonância Magnética , Masculino , Escalas de Graduação Psiquiátrica , Resultado do Tratamento
4.
Med Sci Monit ; 21: 980-6, 2015 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-25836383

RESUMO

BACKGROUND: Liver-specific microRNA (miR)-122 has been shown to be involved in regulating translation of hepatitis C viral (HCV) RNA. This study aimed to explore the molecular mechanism of miR-122 in regulating HCV RNA translation initiation. MATERIAL/METHODS: In human liver hepatocellular carcinoma cell line HepG2, UV cross-link assay was performed on a large scale to identify RNA-binding proteins with gradient concentrations of miR-122. Analytical ultracentrifugation was then used to separate the translation initiation complexes. All RNA-binding proteins were then identified by Western blotting. RESULTS: The binding of 68 kDa protein (p68) to HCV RNA was suppressed by the addition of miR-122 via the competitive binding assay. Such inhibition can be eliminated by the addition of 2'-O-methylated oligonucleotides. This binding suppression was determined to be specific for miR-122, which used the mature single-stranded RNA to suppress the binding of p68 onto HCV RNA. This binding inhibition was further validated by using authentic miR-122 with conserved regions and mutated sequences. CONCLUSIONS: The binding of p68 onto HCV RNA can be specifically inhibited by miR-122 via a competitive binding process.


Assuntos
Hepacivirus/genética , Hepacivirus/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , RNA Viral/genética , RNA Viral/metabolismo , Proteínas de Ligação a RNA/metabolismo , Regiões 5' não Traduzidas , Ligação Competitiva , Células Hep G2 , Humanos , Peso Molecular , Iniciação Traducional da Cadeia Peptídica , Ligação Proteica , Proteínas de Ligação a RNA/química
5.
Asian Pac J Cancer Prev ; 14(6): 3831-6, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23886191

RESUMO

BACKGROUND: S100A14 has recently been implicated in the progress of several types of cancers. This study aimed to investigate the clinical significance and possible mechanisms of action of S100A14 in the invasion and metastasis of hepatocellular carcinoma (HCC). METHODS: S100A14 expression in HCC was detected at mRNA and protein levels and its prognostic significance was assessed. Functional roles of S100A14 in HCC were investigated using MTT, BrdU, wound healing, transwell invasion assay and HCC metastatic mouse model. RESULTS: S100A14 was significantly elevated in HCC tissues, correlated with multiple tumor nodes, high Edmondson-Steiner grade and vascular invasion. Multivariate Cox analysis showed that the S100A14 expression level was a significant and independent prognostic factor for overall survival (OS) of HCC patients (hazard ratio=1.98, 95% confidence interval=1.14-3.46, P=0.013). S100A14 promoted cell proliferation, invasion and metastasis of HCC in vitro and in vivo. CONCLUSION: These results suggest S100A14 is a novel prognostic marker and therapeutic target for HCC.


Assuntos
Biomarcadores Tumorais/metabolismo , Proteínas de Ligação ao Cálcio/metabolismo , Carcinoma Hepatocelular/secundário , Movimento Celular , Proliferação de Células , Neoplasias Hepáticas/patologia , Recidiva Local de Neoplasia/patologia , Adulto , Idoso , Animais , Biomarcadores Tumorais/genética , Western Blotting , Proteínas de Ligação ao Cálcio/genética , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/mortalidade , Feminino , Seguimentos , Humanos , Técnicas Imunoenzimáticas , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/mortalidade , Metástase Linfática , Masculino , Camundongos , Camundongos SCID , Pessoa de Meia-Idade , Gradação de Tumores , Invasividade Neoplásica , Recidiva Local de Neoplasia/metabolismo , Recidiva Local de Neoplasia/mortalidade , Prognóstico , RNA Mensageiro/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Taxa de Sobrevida , Células Tumorais Cultivadas , Cicatrização , Adulto Jovem
6.
Exp Clin Transplant ; 11(4): 352-7, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23121683

RESUMO

OBJECTIVES: To investigate the influence and mechanism of bone marrow mesenchymal stem cell transplant in the synovial proliferation of type II collagen-induced arthritis. MATERIALS AND METHODS: From the bone marrow of Sprague-Dawley rats, mesenchymal stem cells were isolated and expanded. Forty rats were randomly divided into 5 groups: normal control, early mesenchymal stem cell treatment, late mesenchymal stem cell treatment, early collagen-induced arthritis control, and late collagen-induced arthritis control. The mesenchymal stem cells and normal saline were injected through the tail vein, and the following parameters were observed: arthritis index, articular pathology changes, serum vascular endothelial growth factor level, tumor necrosis factor-?, and interluekin-17 levels as detected through stable enzyme-linked immunosorbent assay. RESULTS: The arthritis index and articular pathologic scores of the early and late treatment groups were lower compared with those of the control groups (P < .05). The arthritis index and articular pathologic scores of the late treatment group were lower than those of the early treatment group (P < .05). The levels of vascular endothelial growth factor, tumor necrosis factor-α, and interluekin-17 of the early and late treatment groups were significantly decreased compared with the collagen-induced arthritis control groups (P < .05), and these levels were positively correlated with the arthritis index and articular pathologic scores (P < .05). CONCLUSIONS: The transplant of mesenchymal stem cells in rats with collagen-induced arthritis can inhibit the proliferation of synovium, which may be attributed to the reduced expression of vascular endothelial growth factor, tumor necrosis factor-α, and interluekin-17.


Assuntos
Artrite Experimental/cirurgia , Proliferação de Células , Colágeno Tipo II , Transplante de Células-Tronco Mesenquimais , Membrana Sinovial/patologia , Animais , Artrite Experimental/sangue , Artrite Experimental/induzido quimicamente , Artrite Experimental/patologia , Biomarcadores/sangue , Células Cultivadas , Regulação para Baixo , Interleucina-17/sangue , Masculino , Ratos , Ratos Sprague-Dawley , Membrana Sinovial/metabolismo , Fatores de Tempo , Fator de Necrose Tumoral alfa/sangue , Fator A de Crescimento do Endotélio Vascular/sangue
7.
ISRN Rheumatol ; 2012: 215692, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22548187

RESUMO

Objective. To analyse the potential risk factors of nosocomial infections in patients with active rheumatoid arthritis (RA). Methods. A total of 2452 active RA patients at Hospitals in Shanghai between January 2009 and February 2011 were analyzed. Their demographic and clinical characteristics were compared with those without infection, and the potential risk factors were determined by logistic regression analysis. Results. Multivariate analysis indicated the gender (OR = 0.70, 95% CI 0.53-0.92), duration in hospital (OR = 1.03 , 95%CI 1.01-1.05), number of organs involved (OR = 0.82, 95%CI 0.72-0.92), number of disease-modifying antirheumatic drugs ((DMARDs) (OR = 1.22, 95%CI 1.061-1.40)), corticosteroid therapy (OR = 1.02, 95%CI 1.01-1.03), peripheral white blood cell counts ((WBC) (OR = 1.04, 95%CI 1.00-1.08)), levels of serum albumin (OR = 0.98, 95%CI 0.97-0.99), and C-reactive protein ((CRP) (OR = 1.03 , 95%CI 1.01-1.04)) that were significantly associated with the risk of infections. Conclusion. The female patients, longer hospital stay, more organs involved, more DMARDs, corticosteroid usage, high counts of WBC, lower serum albumin, and higher serum CRP were independent risk factors of infections in active RA patients.

9.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 27(6): 540-2, 2007 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-17633369

RESUMO

OBJECTIVE: A 6-month observation on curative effect of Panlongqi Tablet (PLQ) in treating patients with ankylosing spondylitis (AS) was conducted for evaluating its efficacy and safety. METHODS: One hundred and four inpatients and outpatients with AS were randomly assigned to two groups by using random number table, 52 in each group. All patients were treated non-steroid anti-inflammatory drug (NSAID) and sulfasalazine (SSZ), and those in the treated group were given with PLQ additionally. The change of symptoms and signs were observed, and some laboratory indexes as Bath AS disease activity index (BASDAI), Bath AS functional index (BASFI), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), as well as adverse reaction were observed as well. The revisit and follow-up study were carried out at the 3 months and 6 months respectively. RESULTS: The lumbosacral pain was significantly alleviated, the time for lumbodorsal morning stiffness was shortened, the levels of BASDAI, BASFI, ESR and CRP were significantly lowered after treatment for 3 months or 6months (P < 0.05), and these improvements were more significant in the treated group as compared with those in the control group (P < 0.05). The main adverse reactions was dominantly the gastrointestinal symptoms, which showed insignificant difference between the two groups (P >0.05). CONCLUSION: PLQ is an effective traditional medicine for AS with mild adverse reaction, it can be used as an adjuvant therapy.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Espondilite Anquilosante/tratamento farmacológico , Sulfassalazina/uso terapêutico , Adolescente , Adulto , Anti-Inflamatórios não Esteroides/uso terapêutico , Quimioterapia Combinada , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fitoterapia , Comprimidos , Resultado do Tratamento , Adulto Jovem
10.
Zhonghua Gan Zang Bing Za Zhi ; 14(9): 666-9, 2006 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-16995980

RESUMO

OBJECTIVES: To construct and screen a primarily phage display library of HCV C and E1 genes evolved with an artificial pattern. METHODS: Two genes of about 1 kb with different genotypes were evolved by DNA shuffling. The re-assembled HCV C and E1 genes were cloned into a phage vector. After being rescued with helper phage M13KO7, a phage display library was constructed. Then the library was screened with anti-C and E1 McAb. Double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was carried out on twenty individual phage clones selected randomly to detect their binding and reactive activity with high-titer HCV-positive sera. Normal sera were used as controls. RESULTS: The phage display library of HCV C and E1 genes which evolved with an artificial pattern was constructed. Their capacity amounted to 1.64 x 10(6), and 86 percent of the clones contained C and E1 genes. After four rounds of panning, the phage library was specifically enriched. Twelve positive clones were successfully screened. CONCLUSION: The capacity and diversity of the constructed library are enough for screening. The results demonstrate the superiority of the specific binding and reactive activity and affinity of the 12 phage clones from the HCV positive sera.


Assuntos
DNA Viral/genética , Hepacivirus/genética , Biblioteca de Peptídeos , Proteínas do Core Viral/genética , Proteínas do Envelope Viral/genética , Biblioteca Gênica
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