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1.
Front Microbiol ; 13: 818358, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35547118

RESUMO

Phylogenetic analyses inferred from the nuc rDNA ITS1-5.8S-ITS2 (ITS) data set and the combined 2-locus data set [5.8S + nuc 28S rDNA (nLSU)] of taxa of Trechisporales around the world show that Sistotremastrum family forms a monophyletic lineage within Trechisporales. Bayesian evolutionary and divergence time analyses on two data sets of 5.8S and nLSU sequences indicate an ancient divergence of Sistotremastrum family from Hydnodontaceae during the Triassic period (224.25 Mya). Sistotremastrum family is characterized by resupinate and thin basidiomata, smooth, verruculose, or odontoid-semiporoid hymenophore, a monomitic hyphal structure, and generative hyphae bearing clamp connections, the presence of cystidia and hyphidia in some species, thin-walled, smooth, inamyloid, and acyanophilous basidiospores. In addition, four new species, namely, Trechispora dentata, Trechispora dimitiella, Trechispora fragilis, and Trechispora laevispora, are described and illustrated. In addition, three new combinations, namely, Brevicellicium daweishanense, Brevicellicium xanthum, and Sertulicium limonadense, are also proposed.

2.
Front Vet Sci ; 9: 848833, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35573408

RESUMO

Activation of human immune T-cells by swine leukocyte antigens class I (SLA-I) and class II (SLA-II) leads to xenograft destruction. Here, we generated the GGTA1, B2M, and CIITA (GBC) triple-gene-modified Diannan miniature pigs, analyzed the transcriptome of GBC-modified peripheral blood mononuclear cells (PBMCs) in the pig's spleen, and investigated their effectiveness in anti-immunological rejection. A total of six cloned piglets were successfully generated using somatic cell nuclear transfer, one of them carrying the heterozygous mutations in triple genes and the other five piglets carrying the homozygous mutations in GGTA1 and CIITA genes, but have the heterozygous mutation in the B2M gene. The autopsy of GBC-modified pigs revealed that a lot of spot bleeding in the kidney, severe suppuration and necrosis in the lungs, enlarged peripulmonary lymph nodes, and adhesion between the lungs and chest wall were found. Phenotyping data showed that the mRNA expressions of triple genes and protein expressions of B2M and CIITA genes were still detectable and comparable with wild-type (WT) pigs in multiple tissues, but α1,3-galactosyltransferase was eliminated, SLA-I was significantly decreased, and four subtypes of SLA-II were absent in GBC-modified pigs. In addition, even in swine umbilical vein endothelial cells (SUVEC) induced by recombinant porcine interferon gamma (IFN-γ), the expression of SLA-I in GBC-modified pig was lower than that in WT pigs. Similarly, the expression of SLA-II DR and DQ also cannot be induced by recombinant porcine IFN-γ. Through RNA sequencing (RNA-seq), 150 differentially expressed genes were identified in the PBMCs of the pig's spleen, and most of them were involved in immune- and infection-relevant pathways that include antigen processing and presentation and viral myocarditis, resulting in the pigs with GBC modification being susceptible to pathogenic microorganism. Furthermore, the numbers of human IgM binding to the fibroblast cells of GBC-modified pigs were obviously reduced. The GBC-modified porcine PBMCs triggered the weaker proliferation of human PBMCs than WT PBMCs. These findings indicated that the absence of the expression of α1,3-galactosyltransferase and SLA-II and the downregulation of SLA-I enhanced the ability of immunological tolerance in pig-to-human xenotransplantation.

3.
J Chromatogr A ; 1674: 463145, 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35594798

RESUMO

A comprehensive chemical profiling of traditional Chinese medicine is the basic issue for further pharmacological research and quality assessment. To facilitate chemical identification and potential components discovery, the present study proposed an integrated identification strategy guided by a self-built component database constructed from literatures to carry out the global profiling of complex matrixes. Lanqin Oral Liquid was applied as example to validate the feasibility of this strategy. Based on LQL Component Database containing 710 compounds, modified MDF windows was established to extract the interested analogues, isoquinoline alkaloids, flavonoids and iridoid glycosides, according to their regular integral masses and mass defect. For compounds with characteristic substructures, such as quinic acids, crocins and some glycoside derivatives, the associated neutral losses and diagnostic fragment ions were collected to assist in profiling. Directly matching the m/z or formulas in database was proposed to components with limited regularity of accurate masses and substructures, like indole alkaloids, sesquiterpenes and some nucleosides. Eventually, 170 ions of 1038 precursor ions were identified or temporarily deduced, including 59 alkaloids, 36 flavonoids, 48 terpenoids, 24 organic acids and their derivatives, 2 oligosaccharides, and 1 lignans. Among them, 52 putative compounds were confirmed by chemical standards. The results indicated that the database-oriented identification strategy could locate potential components quickly and eliminate interfering ions, which have the potential for in-depth analysis of compounds.

4.
Connect Tissue Res ; : 1-13, 2022 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-35491814

RESUMO

BACKGROUND: Low back pain is a common symptom of intervertebral disc degeneration (IDD), which seriously affects the quality of life of patients. The abnormal apoptosis and senescence of nucleus pulposus (NP) cells play important roles in the pathogenesis of IDD. Proanthocyanidins (PACs) are polyphenolic compounds with anti-apoptosis and anti-aging effects. However, their functions in NP cells are not yet clear. Therefore, this study was performed to explore the effects of PACs on NP cell apoptosis and aging and the underlying mechanisms of action. METHODS: Cell viability was evaluated by cell counting kit-8 (CCK-8) assay. The apoptosis rate was determined TUNEL assays. Levels of apoptosis-associated molecules (Bcl-2, Bax, C-caspase-3 and Caspase-9) were evaluated via western blot. The senescence was observed through SA-ß-gal staining and western blotting analysis was performed to observe the expression of senescence-related molecules (p-P53, P53, P21 and P16). RESULTS: Pretreatment with PACs exhibited protective effects against IL-1ß-induced NP cell apoptosis including apoptosis rate, expressions of proapoptosis and antiapoptosis related genes and protein. PACs could also alleviate the increase of p-p53, P21, and P16 in IL-1ß-treated NP cells. SA-ß-gal staining showed that IL-1ß-induced senescence of NP cells was prevented by PACs pertreatment. In addition, PACs activated PI3K/Akt pathway in IL-1ß-stimulated NP cells. However, these protected effects were inhibited after LY294002 treatment. CONCLUSION: The results of the present study showed that PACs inhibit IL-1ß-induced apoptosis and aging of NP cells by activating the PI3K/Akt pathway, and suggested that PACs have therapeutic potential for IDD.

5.
JAMA Netw Open ; 5(5): e2210596, 2022 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-35522283

RESUMO

Importance: Promotion of clinician adherence to stroke guidelines can improve stroke outcomes. Objective: To investigate the outcomes of a multilevel system program on clinician adherence to guidelines for treatment of patients with acute ischemic stroke (AIS). Design, Setting, and Participants: This quality improvement study used a prospective interrupted time series (ITS) and difference-in-difference (DID) design, from August 1, 2018, to January 31, 2020, divided into preprogram term and short and long postprogram terms; each term had 6 months. Data were collected during hospitalization and at discharge with an automated medical record data capture system in 58 public hospitals in Zhejiang province, China. Data were analyzed from August 2018 to January 2020. Exposures: The multilevel system program included a modularized standard template for medical records, centrally supported continuing education, continuous monitoring and feedback, and collaborative workshops. Main Outcomes and Measures: The primary outcome was adherence to 12 key performance indicators (KPIs), expressed as (1) percentage of patient-applicable KPIs achieved in each participant and (2) percentage of participants among whom all applicable KPIs were achieved (dichotomous all-or-none measure). The secondary outcome was severe disability or death (modified Rankin Scale 5-6) at discharge. Results: Among 45 091 patients (mean [SD] age, 69 [12] years; 18 347 female [40.7%]), 28 721 from 30 hospitals received the program and 16 370 from 28 hospitals continued routine care. In adjusted DID analysis, the program was associated with an increase in the absolute percentage of KPIs achieved per patient (6.46%; 95% CI, 5.49% to 7.43%), absolute rate of all-or-none success (8.29%; 95% CI, 6.99% to 9.60%), and decreased rate of severe disability or death at discharge (-1.68%; 95% CI, -2.99% to -0.38%). The ITS result showed the program was associated with an increase in KPIs achieved per patient per week (slope change in short-term period, 0.36%; 95% CI, 0.20% to 0.52%; level change in long-term period, (9.64%; 95% CI, 4.58% to 14.69%) and in all-or-none success (slope change in short-term period 0.34%; 95% CI, 0.23% to 0.46%; level change in long-term period 5.89%; 95% CI, 0.19% to 11.59%). Conclusions and Relevance: The centrally supported program was associated with increases in clinician adherence to guidelines and reduced the proportion of severely disabled or deceased patients with AIS at discharge, providing support for its wider implementation.


Assuntos
AVC Isquêmico , Acidente Vascular Cerebral , Idoso , Feminino , Hospitais , Humanos , AVC Isquêmico/terapia , Masculino , Estudos Prospectivos , Melhoria de Qualidade , Acidente Vascular Cerebral/terapia
6.
Micromachines (Basel) ; 13(4)2022 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-35457869

RESUMO

X-ray imaging machines are widely used in border control checkpoints or public transportation, for luggage scanning and inspection. Recent advances in deep learning enabled automatic object detection of X-ray imaging results to largely reduce labor costs. Compared to tasks on natural images, object detection for X-ray inspection are typically more challenging, due to the varied sizes and aspect ratios of X-ray images, random locations of the small target objects within the redundant background region, etc. In practice, we show that directly applying off-the-shelf deep learning-based detection algorithms for X-ray imagery can be highly time-consuming and ineffective. To this end, we propose a Task-Driven Cropping scheme, dubbed TDC, for improving the deep image detection algorithms towards efficient and effective luggage inspection via X-ray images. Instead of processing the whole X-ray images for object detection, we propose a two-stage strategy, which first adaptively crops X-ray images and only preserves the task-related regions, i.e., the luggage regions for security inspection. A task-specific deep feature extractor is used to rapidly identify the importance of each X-ray image pixel. Only the regions that are useful and related to the detection tasks are kept and passed to the follow-up deep detector. The varied-scale X-ray images are thus reduced to the same size and aspect ratio, which enables a more efficient deep detection pipeline. Besides, to benchmark the effectiveness of X-ray image detection algorithms, we propose a novel dataset for X-ray image detection, dubbed SIXray-D, based on the popular SIXray dataset. In SIXray-D, we provide the complete and more accurate annotations of both object classes and bounding boxes, which enables model training for supervised X-ray detection methods. Our results show that our proposed TDC algorithm can effectively boost popular detection algorithms, by achieving better detection mAPs or reducing the run time.

7.
J Thorac Dis ; 14(3): 741-748, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35399241

RESUMO

Background: Uniportal video-assisted thoracoscopic surgery (VATS) segmentectomy is widely used in the field of thoracic surgery. However, anatomical variations in the bronchi and lung vessels may be critical obstacles during precise pulmonary segmentectomy. Thus, it is necessary to optimize uniportal VATS segmentectomy and to accurately identify the plane between lung segments by precisely transecting the bronchi and blood vessels of the lung segments. The indocyanine green fluorescence (ICGF)-based method has the potential to be a feasible and effective technique to facilitate the uniportal VATS segmentectomy. The present study aims at comparing the short-term outcomes of ICGF versus the traditional inflation-deflation method for uniportal VATS segmentectomy. Methods: The perioperative clinical data in 200 consecutive patients undergoing uniportal VATS segmentectomy from December 2018 to August 2020 at Shanghai Chest Hospital were analyzed retrospectively. The targeted segment structures were identified and dissected precisely by using ICGF-based (N=100) or the traditional inflation-deflation (N=100) methods. The parameters of intraoperative blood loss and operation time, postoperative drainage volume, air leakage time, drainage tube retention time, length of hospital stay, and complications in the ICGF group were collected. Further, the operation time between the ICGF and the inflation-deflation groups was compared. The data summary and statistical analysis were performed by SPSS 19.0. P value <0.05 was considered statistically significant. Results: No massive hemorrhage, hypoxemia, allergy, conversion to lobectomy, or wedge resection was noted during the surgery. ICGF groups resulted in a shorter operative time (90±11.46 vs. 118±10.59 min, P<0.001). No postoperative complications were observed, e.g., bronchopleural fistula, hemoptysis, or atelectasis. All patients were discharged as routinely scheduled. No disease recurrence or metastasis was found during the follow-ups. Conclusions: Our study indicated that the ICGF-based navigation approach is a simple, effective, and reliable technique that can greatly facilitate the uniportal VATS segmentectomy.

8.
Chemosphere ; 301: 134713, 2022 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-35487350

RESUMO

Rational design of all-solid-state Z-scheme heterojunction with advanced structure is essential for boosting photocatalytic efficiency. Herein, we design and fabricate a novel Z-scheme photocatalyst with leaf architecture (named artificial leaf) via a simple dipping-calcination (DC) process followed by a successive ionic layer adsorption and reaction (SILAR) strategy. The prepared artificial leaf, composing of CdS, InVO4, and BiVO4, holds advanced leaf-like structure and Z-scheme electron transfer pathway. As a result, this novel artificial leaf exhibits outstanding capability for the harvesting of visible light and superior efficiency for the separation of photogenerated electron-hole pairs, as well as remarkably enhanced photocatalytic performance and stability for H2 evolution (with the rate of 5033 µm g-1∙h-1) and pollution degradation (46% pollution can be degraded within 3 h).

9.
Thorac Cancer ; 13(8): 1176-1183, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35261166

RESUMO

BACKGROUND: The lobar and segmental anatomy are the basis for anatomical pulmonary segmentectomy. METHODS: From October 2017 to June 2021, 136 patients with small pulmonary nodules scheduled for anatomical pulmonary segmentectomy at our institution underwent three-dimensional (3D) lung reconstruction. The anatomy of the left upper lobe (LUL) was statistically analyzed and graphically mapped using the reconstructed models, and the role of this reconstruction method in performing pulmonary segmentectomy was explored. RESULTS: Through the analysis of the reconstructed models, the upper stem (S1 + 2 + 3) bronchus was classified as having two (94/136 cases) or three branches (42/136 cases). The upper stem artery had two branches in 24/136 patients, three in 60/136 cases, four in 44/136 cases, and five in 8/136 cases. A total of 103/136 upper stem veins had two branches, 26/136 had three branches, and 7/136 had four branches. The lingual stem (S4 + 5) bronchus was two-branched in 116/136 cases and three-branched in 20/136 cases, while the lingual artery was single-branched in 61/136 cases, two-branched in 70/136 cases, and three-branched in rare cases (5/136 cases). The lingual stem vein was unbranched in 119/136 cases and two-branched in 17/136 cases. Additionally, six unusual variants (<5%) were identified: one in the bronchus, with four cases; three in the pulmonary artery, with six cases; and two in the pulmonary vein, with two cases. CONCLUSIONS: 3D reconstruction can yield results similar to specimens for lung segment studies. The reconstruction strategy and the data presented in this article will be valuable references for thoracic surgeons performing anatomic resections.


Assuntos
Imageamento Tridimensional , Veias Pulmonares , Humanos , Imageamento Tridimensional/métodos , Pulmão/irrigação sanguínea , Pulmão/diagnóstico por imagem , Pulmão/cirurgia , Artéria Pulmonar/cirurgia , Tomografia Computadorizada por Raios X/métodos
10.
Biophys Chem ; 284: 106782, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35231820

RESUMO

Polyglutamine (polyQ) model peptides are ideally suited to analyze the involvement of glutamines in the disease-related aggregation onset. Here we use a template-assisted design of polyQ-rich hairpin peptides (Trpzip-Qn) to monitor structural stability with fluorescence spectroscopy. The hairpin model imitates the monomeric motif of a polyQ fibril and is stabilized by hydrophobic interactions of two cross-strand pairs of tryptophans (Trps) which are used as fluorophores to report on structural changes. The Trps also frame the polyQ repeats located on each hairpin strand with a different number of glutamines (Qn). Single-stranded sequences mimic the unfolded state and were used as references to differentiate the intrinsic fluorescence signal from the spectral effect caused by structural changes. Temperature-induced hairpin unfolding was monitored by the spectral shift of the Trp fluorescence signal and transition temperatures were determined. The magnitude of the spectral shift indicates the degree of structural disorder. We observed that a longer polyQ repeat is more disordered and weakens the cross-strand Trp-Trp interactions resulting in a decrease of the spectral shift. Aggregation to a fibrillar and more ordered structure shows an increase of the spectral shift. In addition, a band at 280 nm occurs in the spectrum which clearly correlates with the turbidity of the sample and is attributed to scattering of larger aggregated structures. Our study reveals that the number of glutamines, pH and temperature affect structural stability and aggregation of polyQ repeats.


Assuntos
Peptídeos , Triptofano , Peptídeos/química , Temperatura
11.
PLoS One ; 17(3): e0263706, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35275910

RESUMO

As an emerging charity model, critical illness network crowdfunding provides a source of funds for some critically ill patients in China who have difficulty paying their high treatment costs by themselves. This study aims to investigate the influencing factors of the public's willingness to donate to critical illness crowdfunding projects on Internet platforms. From a perspective combining the technology acceptance model and the theory of planned behavior, a complex and comprehensive structural equation model is proposed. We randomly selected 1,000 members of the public in China and empirically verified the study framework through structural equation modeling (SEM) based on 710 valid questionnaires. The results show that the public's donation willingness and the social distance to a critical illness crowdfunding project on an online platform positively affect the public's donation behavior, and donation attitude positively affect donation willingness; perceived usefulness and empathic concern positively affect the public's donation attitude, which in turn affects its donation willingness. This study confirms that members of the public are more likely to help people who have similar demographic factors or people who are similar to themselves and have the same values, i.e., people who have a close social distance. It innovatively proposes and verifies the hypothesis that empathic concern can significantly positively affect users' perceived usefulness and donation attitude. Strong empathic concern triggers donation willingness and behavior.


Assuntos
Estado Terminal , Administração Financeira , Atitude , China , Humanos , Inquéritos e Questionários
12.
ACS Appl Mater Interfaces ; 14(11): 13836-13847, 2022 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-35286068

RESUMO

Both 3-hydroxy-2-butanone and triethylamine are highly toxic and harmful to human health, and their chronic inhalation can cause respiratory diseases, eye lesions, dermatitis, headache, dizziness, drowsiness, and even fatality. Developing sensors for detecting such toxic gases with low power consumption, high response with superselectivity, and stability is crucial for healthcare and environmental monitoring. This study presents a typical gas sensor fabricated based on AuPdO modified Cu-doped K2W4O13 nanowires, which can selectively detect 3-hydroxy-2-butanone and triethylamine at 120 and 200 °C, respectively. The sensor displays excellent sensing performance at reduced operating temperature, high selectivity, fast response/recovery, and stability, which can be attributed to a synergistic effect of Cu dopants and AuPdO nanoparticles on the K2W4O13 host. The enhanced sensing response and selectivity could be attributed to the oxygen vacancies/defects, bandgap excitation, the electronic sensitization, the reversible redox reaction of PdO and Cu, the cocatalytic activity of AuPdO, and Schottky barrier contacts at the interface of tungsten oxide and Au. The significant variations in the activation capacities of Cu-doped K2W4O13, Pd/PdO, and Au nanoparticles toward 3H-2B and TEA, and the diffusion depth of the two gases in the coated sensing layer may cause dual selectivity. The designed gas sensor materials can serve as a sensitive target for detecting toxic biomarkers and hold broad application prospects in food and environmental safety inspection.

13.
Chin Med ; 17(1): 27, 2022 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-35193642

RESUMO

BACKGROUND: Tea trees originated in southwest China 60 million or 70 million years ago. Written records show that Chinese ancestors had begun drinking tea over 3000 years ago. Nowadays, with the aging of populations worldwide and more people suffering from non-communicable diseases or poor health, tea beverages have become an inexpensive and fine complementary and alternative medicine (CAM) therapy. At present, there are 3 billion people who like to drink tea in the world, but few of them actually understand tea, especially on its development process and the spiritual and cultural connotations. METHODS: We searched PubMed, Google Scholar, Web of Science, CNKI, and other relevant platforms with the key word "tea", and reviewed and analyzed tea-related literatures and pictures in the past 40 years about tea's history, culture, customs, experimental studies, and markets. RESULTS: China is the hometown of tea, tea trees, tea drinking, and tea culture. China has the oldest wild and planted tea trees in the world, fossil of a tea leaf from 35,400,000 years ago, and abundant tea-related literatures and art works. Moreover, tea may be the first Chinese herbal medicine (CHM) used by Chinese people in ancient times. Tea drinking has many benefits to our physical health via its antioxidant, anti-inflammatory, immuno-regulatory, anticancer, cardiovascular-protective, anti-diabetic, and anti-obesity activities. At the moment, COVID-19 is wreaking havoc across the globe and causing severe damages to people's health and lives. Tea has anti-COVID-19 functions via the enhancement of the innate immune response and inhibition of viral growth. Besides, drinking tea can allow people to acquire a peaceful, relaxed, refreshed and cheerful enjoyment, and even longevity. According to the meridian theory of traditional Chinese medicine, different kinds of tea can activate different meridian systems in the human body. At present, black tea (fermented tea) and green tea (non-fermented tea) are the most popular in the world. Black tea accounts for over 90% of all teas sold in western countries. The world's top-grade black teas include Qi Men black in China, Darjeeling and Assam black tea in India, and Uva black tea in Sri Lanka. However, all top ten famous green teas in the world are produced in China, and Xi Hu Long Jing tea is the most famous among all green teas. More than 700 different kinds of components and 27 mineral elements can be found in tea. Tea polyphenols and theaflavin/thearubigins are considered to be the major bioactive components of black tea and green tea, respectively. Overly strong or overheated tea liquid should be avoided when drinking tea. CONCLUSIONS: Today, CAM provides an array of treatment modalities for the health promotion in both developed and developing countries all over the world. Tea drinking, a simple herb-based CAM therapy, has become a popular man-made non-alcoholic beverage widely consumed worldwide, and it can improve the growth of economy as well. Tea can improve our physical and mental health and promote the harmonious development of society through its chemical and cultural elements.

14.
Thorac Cancer ; 13(6): 795-803, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35142044

RESUMO

BACKGROUND: Three-dimensional reconstruction of chest computerized tomography (CT) excels in intuitively demonstrating anatomical patterns for pulmonary segmentectomy. However, current methods are labor-intensive and rely on contrast CT. We hereby present a novel fully automated reconstruction algorithm based on noncontrast CT and assess its performance both independently and in combination with surgeons. METHODS: A retrospective pilot study was performed. Patients between May 2020 to August 2020 who underwent segmentectomy in our single institution were enrolled. Noncontrast CTs were used for reconstruction. In the first part of the study, the accuracy of the demonstration of anatomical variants by either automated or manual reconstruction algorithm were compared to surgical observation, respectively. In the second part of the study, we tested the accuracy of the identification of anatomical variants by four independent attendees who reviewed 3-D reconstruction in combination with CT scans. RESULTS: A total of 20 cases were enrolled in this study. All segments were represented in this study with two left S1-3, two left S4 + 5, one left S6, five left basal segmentectomies, one right S1, three right S2, 1 right S2b + 3a, one right S3, two right S6 and two right basal segmentectomies. The median time consumption for the automated reconstruction was 280 (205-324) s. Accurate vessel and bronchial detection were achieved in 85% by the AI approach and 80% by Mimics, p = 1.00. The accuracy of vessel classification was 80 and 95% by AI and manual approaches, respectively, p = 0.34. In real-world application, the accuracy of the identification of anatomical variant by thoracic surgeons was 85% by AI+CT, and the median time consumption was 2 (1-3) min. CONCLUSIONS: The AI reconstruction algorithm overcame defects of traditional methods and is valuable in surgical planning for segmentectomy. With the AI reconstruction, surgeons may achieve high identification accuracy of anatomical patterns in a short time frame.


Assuntos
Neoplasias Pulmonares , Pneumonectomia , Algoritmos , Humanos , Pulmão/diagnóstico por imagem , Pulmão/cirurgia , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/cirurgia , Projetos Piloto , Pneumonectomia/métodos , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos
15.
Front Immunol ; 13: 826880, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35185917

RESUMO

Cyclic GMP-AMP synthase (cGAS) recognizes double-stranded DNA (dsDNA) derived from invading pathogens and induces an interferon response via activation of the key downstream adaptor protein stimulator of interferon genes (STING). This is the most classic biological function of the cGAS-STING signaling pathway and is critical for preventing pathogenic microorganism invasion. In addition, cGAS can interact with various types of nucleic acids, including cDNA, DNA : RNA hybrids, and circular RNA, to contribute to a diverse set of biological functions. An increasing number of studies have revealed an important relationship between the cGAS-STING signaling pathway and autophagy, cellular senescence, antitumor immunity, inflammation, and autoimmune diseases. This review details the mechanism of action of cGAS as it interacts with different types of nucleic acids, its rich biological functions, and the potential for targeting this pathway to treat various diseases.


Assuntos
Inflamação/etiologia , Proteínas de Membrana/fisiologia , Ácidos Nucleicos/classificação , Nucleotidiltransferases/fisiologia , Animais , Doenças Autoimunes/imunologia , Autofagia/fisiologia , Senescência Celular , DNA/metabolismo , Humanos , Interferon Tipo I/fisiologia , Transdução de Sinais/fisiologia
16.
Cell Chem Biol ; 2022 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-35093210

RESUMO

Identification of the tyrosine phosphorylation (pY)-dependent interactome of immune co-receptors is crucial for understanding signal pathways involved in immunotherapy. However, identifying the motif-specific interactome for each pY commonly found on these multi-phosphorylated membrane proteins remains challenging. Here, we describe a photoaffinity-based chemical proteomic approach to dissect the motif-specific cytoplasmic interactomes of the critical immune co-receptor CD28. Various full-length CD28 cytoplasmic tails (CD28cyto) with defined pY and selectively replaced photo-methionine were synthesized and applied to explore three pY-motif-dependent CD28cyto interactomes. We identified a stand-alone interaction of phospholipase PLCG1 with the Y191 motif with enhanced affinity for the sequence neighboring the transmembrane domain. Importantly, taking advantage of native top-down mass spectrometry with a 193-nm laser, we discovered the direct association of a previously undefined pY218 motif with the kinase PKCθ through its C2 domain. This synthetic CD28cyto-based photoaffinity proteomic approach is generically applicable to the study of other immune co-receptors with multiple pY sites on their linear cytoplasmic tail.

17.
Risk Anal ; 2022 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-35030642

RESUMO

The management of human and organizational factors (HOFs) within the public sector directly concerns the efficacy of epidemic prevention and control (EPC). Insufficient examination of such HOFs has led to defective countermeasures. This study attempts to comprehensively identify the HOFs within the public sector critical to EPC and investigate their interactions with the weighted network theory. A total of 55 HOFs were identified, and their interactions were assessed and visualized in the Chinese context. Then, the established weighted network was analyzed to investigate the interactions and diagnose critical factors and sectors. The analysis shows that there are strong interactions among HOFs, and that the human and organizational risks emerging from administrative departments of public health, centers for disease control and prevention, and medical institutions act as the key risk sources in the complex interconnected EPC system, exacerbating risk and causing a significant domino effect. It is recommended that the authorities devote more resources to the core sectors and endeavor to reinforce those critical HOFs by implementing closer risk communication, collaboration, and response. This study may deepen and broaden the authorities' awareness and understanding of interactions among HOFs regarding epidemic mitigation, and strengthen their capacity to perceive, evaluate, and manage these factors in a proactive and effective way, thereby facilitating the success of EPC.

18.
Mol Med Rep ; 25(2)2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-35014689

RESUMO

Triple­negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, and it often becomes resistant to paclitaxel (PTX) therapy. Autophagy plays an important cytoprotective role in PTX­induced tumor cell death, and targeting autophagy has been promising for improving the efficacy of tumor chemotherapy in recent years. The aim of the present study was to clarify the mechanism of PTX inducing autophagy in TNBC cells to provide a potential clinical chemotherapy strategy of PTX for TNBC. The present study reported that PTX induced both apoptosis and autophagy in MDA­MB­231 cells and that inhibition of autophagy promoted apoptotic cell death. Furthermore, it was found that forkhead box transcription factor O1 (FOXO1) enhanced PTX­induced autophagy through a transcriptional activation pattern in MDA­MB­231 cells, which was associated with the downstream target genes autophagy related 5, class III phosphoinositide 3­kinase vacuolar protein sorting 34, autophagy related 4B cysteine peptidase, beclin 1 and microtubule associated protein 1 light chain 3ß. Knocking down FOXO1 attenuated the survival of MDA­MB­231 cells in response to PTX treatment. These findings may be beneficial for improving the treatment efficacy of PTX and to develop autophagic targeted therapy for TNBC.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Proteína Forkhead Box O1/metabolismo , Paclitaxel/farmacologia , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Neoplasias de Mama Triplo Negativas/metabolismo , Adenina/análogos & derivados , Adenina/farmacologia , Proteína 5 Relacionada à Autofagia/metabolismo , Proteína Beclina-1/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Classe III de Fosfatidilinositol 3-Quinases/metabolismo , Cisteína Endopeptidases/metabolismo , Resistencia a Medicamentos Antineoplásicos/fisiologia , Proteína Forkhead Box O1/genética , Humanos , Proteínas Associadas aos Microtúbulos/metabolismo
19.
Transgenic Res ; 31(1): 59-72, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34741281

RESUMO

Leptin is a well-known adipokine that plays critical role in adiposity. To further investigate the role of leptin in adiposity, we utilized leptin overexpressing transgenic pigs and evaluated the effect of leptin on growth and development, fat deposition, and lipid metabolism at tissue and cell level. Leptin transgenic pigs were produced and divided into two groups: elevated leptin expression (leptin ( +)) and normal leptin expression group (control). Results indicated that leptin ( +) pigs had elevated leptin protein and mRNA expression levels and exhibited sluggish growth and development followed by decreased subcutaneous fat thickness, low serum triglycerides, saturated, unsaturated fatty acids and high cholesterol esters (p < 0.05). There were differences in the lipid metabolism related genes at different fat depots, including upregulation of PPARγ, AGPAT6, PLIN2, HSL and ATGL in subcutaneous, PPARγ in perirenal, and FAT/CD36 and PLIN2 in mesenteric adipose tissues and downregulation of AGPAT6 and ATGL in perirenal and AGPAT6 in mesenteric adipose tissues (p < 0.05). Additionally, in-vitro cultured leptin ( +) preadipocytes exhibited upregulation of PPARγ, FAT/CD36, ACACA, AGPAT, PLIN2, ATGL and HSL as compared to control (p < 0.05). These findings suggested that homeostasis imbalance in lipolysis and lipogenesis at adipose tissue and adipocytes levels led to low subcutaneous fat depots in leptin overexpression pigs. These pigs can act as model for obesity and related metabolic disorder.


Assuntos
Leptina , PPAR gama , Tecido Adiposo/metabolismo , Animais , Leptina/genética , Leptina/metabolismo , Lipólise , Obesidade/genética , PPAR gama/genética , PPAR gama/metabolismo , PPAR gama/farmacologia , Suínos/genética , Triglicerídeos/genética
20.
Adv Mater ; 34(7): e2105114, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34847252

RESUMO

Power conversion efficiency (PCE) of organic solar cells (OSCs) has crossed the 18% mark for OSCs, which are largely fabricated by spin-coating, and the optimal photoactive thickness is limited to 100 nm. To increase reproducibility of results with industrial roll-to-roll (R2R) processing, slot-die coating coupled with a ternary strategy for optimal performance of large-area, thick OSCs is used. Based on miscibility differences, a highly crystalline molecule, BTR-Cl, is incorporated, and the phase-separation kinetics of the D18:Y6 film is regulated. BTR-Cl provides an early liquid-liquid phase separation and early aggregation of Y6, which slightly improves the molecular crystallinity and vertical phase separation of the ternary blends, resulting in high PCEs of 17.2% and 15.5% for photoactive films with thicknesses of 110 and 300 nm, respectively. The ternary design strategy for large-area and thick films is further used to fabricate high-efficiency flexible devices, which promises reproducibility of the lab results from slot-die coating to industrial R2R manufacturing.

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