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1.
Huan Jing Ke Xue ; 43(6): 3140-3148, 2022 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-35686783

RESUMO

In recent years, non-point source pollution has become the main cause of water quality deterioration in some reservoirs in China. Taking the Panjiakou Reservoir as an example, the classical output risk model was improved by introducing a precipitation factor and terrain factor. Combined with high-resolution satellite precipitation products (GPM) and GF-6 satellite images, a high-resolution data-driven risk assessment method for non-point source pollution output was established to study the temporal and spatial distribution characteristics of non-point source pollution output risk in the Panjiakou Reservoir basin. The results showed that the non-point source pollution output risk was high in the study area in 2018. The areas with higher and highest risk of nitrogen pollution output accounted for approximately 70.6% of the total watershed area, whereas the higher risk of phosphorus pollution output accounted for approximately 21.9%. The temporal and spatial distribution characteristics of non-point source pollution output risk in the Panjiakou Reservoir basin were analyzed. It was found that the non-point source pollution output risk in the Panjiakou Reservoir basin increased first and then decreased from April to September. This was consistent with the spatial and temporal distribution of precipitation in the basin. Combined with the analysis of land use distribution characteristics, the upstream area of the basin was mainly cultivated land, whereas cities were concentrated in the downstream portion of the basin. Affected by agricultural production and human activities, the risk of non-point source pollution output was higher in these regions. In view of the temporal and spatial distribution characteristics of non-point source pollution output risk, it is necessary to formulate a reasonable agricultural fertilization method, plan the landscape layout of source-sinks, and construct vegetation buffer zones.


Assuntos
Poluição Difusa , Poluentes Químicos da Água , China , Monitoramento Ambiental/métodos , Humanos , Nitrogênio/análise , Fósforo/análise , Medição de Risco , Poluentes Químicos da Água/análise , Qualidade da Água
2.
Huan Jing Ke Xue ; 43(6): 3241-3252, 2022 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-35686794

RESUMO

To clarify the distribution characteristics and the ecological stoichiometric characteristics of nutrient elements in soils under different vegetation types, four typical natural wetlands, i.e., Phragmites australis wetland, Tamarix chinensis wetland, Suaeda salsa wetland, and Tidal flat wetland, as well as Gossypium spp. fields that were reclaimed from natural wetlands, were selected as study sites in the Yellow River Delta, and comparisons between the agricultural reclamation land and natural wetlands were conducted. The results showed that the soil total organic carbon (TOC) and total nitrogen (TN) contents in the natural wetlands were as follows:P. australis wetland and T. chinensis wetland>S. salsa wetland>Tidal flat, and the contents of TOC and TN were significantly negatively related to electrical conductivity (EC) and pH values (P<0.05). The contents of TOC, TN, and total phosphorus (TP) in Gossypium spp. fields were significantly higher than those in natural wetlands (P<0.05), especially the contents of nitrate nitrogen (NO3--N) in Gossypium spp. fields, which were 9.4-11.4 times that of natural wetlands. However, no significant correlations between TOC, TN, and TP and EC and pH values (P>0.05) were observed in Gossypium spp. fields. The results of correlation analysis showed that the C/N of natural wetlands were mainly controlled by the contents of TN (P<0.05), and the C/N of the Gossypium spp. fields were significantly lower than those of natural wetlands (P<0.05). The soil C/P and N/P of natural wetlands and Gossypium spp. fields in the Yellow River Delta were low, and the variation trends were consistent with those of soil TOC and TN. Comparative analysis revealed, on the whole, that there were significantly different soil nutrient element contents, C/N, C/P, and N/P in Gossypium spp. fields compared to those of natural wetlands (P<0.05). The process of reclamation could significantly change the spatial distribution of nutrient elements in wetlands. Our results should be of importance in revealing the biogeochemical process of soil nutrient elements in coastal wetland and the influence of agricultural reclamation activities on the differentiation of soil nutrient elements.


Assuntos
Solo , Áreas Alagadas , Carbono/análise , China , Nitrogênio/análise , Nutrientes/análise , Fósforo/análise , Rios/química , Solo/química
3.
Opt Lett ; 47(11): 2927-2930, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35648966

RESUMO

We propose a sign-singularity solution in single-shot digital speckle interferometry based on vortex-phase modulation. The vortex phase is introduced through the reference beam as variation information by a reflective liquid crystal spatial light modulator. The sign singularity is eliminated with the help of a single spiral speckle fringe pattern. To effectively obtain the deformation phase, the measurement process is specially designed on the consideration of the modulation principle of the vortex phase, which is discussed in terms of theoretical analysis and simulation results. Experiments on out-of-plane deformation measurement reveal the feasibility of the proposed method and suggest the potential in actual dynamic measurement applications.

4.
J Chem Inf Model ; 2022 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-35675668

RESUMO

Accurate estimation of the synthetic accessibility of small molecules is needed in many phases of drug discovery. Several expert-crafted scoring methods and descriptor-based quantitative structure-activity relationship (QSAR) models have been developed for synthetic accessibility assessment, but their practical applications in drug discovery are still quite limited because of relatively low prediction accuracy and poor model interpretability. In this study, we proposed a data-driven interpretable prediction framework called GASA (Graph Attention-based assessment of Synthetic Accessibility) to evaluate the synthetic accessibility of small molecules by distinguishing compounds to be easy- (ES) or hard-to-synthesize (HS). GASA is a graph neural network (GNN) architecture that makes self-feature deduction by applying an attention mechanism to automatically capture the most important structural features related to synthetic accessibility. The sampling around the hypothetical classification boundary was used to improve the ability of GASA to distinguish structurally similar molecules. GASA was extensively evaluated and compared with two descriptor-based machine learning methods (random forest, RF; eXtreme gradient boosting, XGBoost) and four existing scores (SYBA: SYnthetic Bayesian Accessibility; SCScore: Synthetic Complexity score; RAscore: Retrosynthetic Accessibility score; SAscore: Synthetic Accessibility score). Our analysis demonstrates that GASA achieved remarkable performance in distinguishing similar molecules compared with other methods and had a broader applicability domain. In addition, we show how GASA learns the important features that affect molecular synthetic accessibility by assigning attention weights to different atoms. An online prediction service for GASA was offered at http://cadd.zju.edu.cn/gasa/.

5.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(5): 530-535, 2022 May 15.
Artigo em Chinês | MEDLINE | ID: mdl-35644193

RESUMO

OBJECTIVES: To study the clinical features of intestinal polyps and the risk factors for secondary intussusception in children. METHODS: A retrospective analysis was performed for the medical data of 2 669 children with intestinal polyps. According to the presence or absence of secondary intussusception, they were divided into two groups: intussusception (n=346) and non-intussusception (n=2 323). Related medical data were compared between the two groups. The multivariate logistic regression analysis was used to identify the risk factors for secondary intussusception. RESULTS: Among the children with intestinal polyps, 62.42% were preschool children, and the male/female ratio was 2.08∶1; 92.66% had hematochezia as disease onset, and 94.34% had left colonic polyps and rectal polyps. There were 346 cases of secondary intussusception, with an incidence rate of 12.96% (346/2 669). Large polyps (OR=1.644, P<0.001), multiple polyps (≥2) (OR=6.034, P<0.001), and lobulated polyps (OR=93.801, P<0.001) were the risk factors for secondary intussusception. CONCLUSIONS: Intestinal polyps in children often occur in preschool age, mostly in boys, and most of the children have hematochezia as disease onset, with the predilection sites of the left colon and the rectum. Larger polyps, multiple polyps, and lobulated polyps may increase the risk of secondary intussusception, and endoscopic intervention is needed as early as possible to improve prognosis.


Assuntos
Intussuscepção , Pré-Escolar , Feminino , Hemorragia Gastrointestinal , Humanos , Pólipos Intestinais/complicações , Intussuscepção/complicações , Masculino , Estudos Retrospectivos , Fatores de Risco
6.
Br J Hosp Med (Lond) ; 83(5): 1-16, 2022 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-35653318

RESUMO

Heart failure has many causes. Although new drugs, devices and technologies are available, the survival rate and prognosis of patients with heart failure remain poor, placing a significant burden on individuals and society. Attempts to improve outcomes for patients with heart failure include developing prognostic risk scores. With medical advances, however, previous heart failure risk scores are not fully applicable to current practice, particularly because of the classification as heart failure with reduced ejection fraction, heart failure with mildly reduced ejection fraction, and heart failure with preserved ejection fraction. This article describes the use of risk prediction scores for heart failure patients with different clinical status and discusses their clinical applicability.


Assuntos
Insuficiência Cardíaca , Disfunção Ventricular Esquerda , Insuficiência Cardíaca/diagnóstico , Humanos , Prognóstico , Fatores de Risco , Volume Sistólico
7.
Foot Ankle Int ; : 10711007221099183, 2022 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-35658553

RESUMO

BACKGROUND: To compare the clinical/functional outcomes of supramalleolar osteotomy (SMOT) and ankle arthrodesis (AA) for the treatment of modified Takakura stage 3B ankle osteoarthritis. METHODS: Outcomes of 28 SMOT patients and 30 AA patients were reviewed at an average of 50 and 51 months, respectively. The baseline characteristics of the 2 groups were similar. The preoperative tibial articular surface angle and talar tilt angle in the SMOT group were 82.6 and 10 degrees and in the AA group, 83.9 and 9.1 degrees, respectively. The American Orthopaedic Foot & Ankle Society (AOFAS) ankle-hindfoot score, visual analog scale (VAS) score, 12-item Short-Form Health Survey (SF-12) mental component summary (MCS) and physical component summary (PCS) scores, range of motion (ROM), radiologic parameters, and complications were compared. RESULTS: The AOFAS, VAS, and SF-12 MCS and PCS scores improved significantly postoperatively in both groups (P < .001). The VAS and SF-12 PCS scores indicate marginally better improvement in the AA group (P < .05). The patient satisfaction value (P = .028) and the possibility of repeated surgery value (P = .012) were also significantly higher in the AA group. The early (P = .905) and late (P = .181) complications did not significantly differ between the 2 groups. The reoperation rate was significantly higher in the SMOT group (P = .038). CONCLUSION: Both SMOT and AA showed improvements in function, pain, alignment, and quality of life after surgery. Patients in the AA group reported better pain relief, had a lower reoperation rate, and better hindfoot alignment during a short- to mid-term follow-up time. LEVEL OF EVIDENCE: Level III, retrospective comparative study.

8.
Gastrointest Endosc ; 2022 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-35718070

RESUMO

BACKGROUND AND AIMS: The clinical application of gastrointestinal endoscopy for the diagnosis of multiple diseases using artificial intelligence (AI) has been limited by its high false positive rates. There is an unmet need to develop a Gastrointestinal Endoscopy AI-assisted Diagnosis System (GEADS) to improve diagnostic accuracy and clinical utility. METHODS: In this retrospective, multicenter study, a convolutional neural network was trained to assess upper gastrointestinal diseases based on 26,228 endoscopic images from Dazhou Central Hospital that were randomly assigned (3:1:1) to the training dataset, validation dataset, and test dataset, respectively. To validate the model, 6 external independent datasets comprising 51,372 images of upper gastrointestinal diseases were collected. Besides, one prospective dataset comprising 27,975 images were collected. The performance of GEADS was compared with endoscopists with two professional degrees of expertise: expert, novice. 8 endoscopists in the expert group with more than 5 years of experience, 3 endoscopists in the novice group with 1 to 5 years of experience. RESULTS: The GEADS model achieved an accuracy of 0.918 (95% confidence interval [CI]: 0.914-0.922), with an F1 score of 0.884 (95% CI: 0.879-0.889), recall of 0.873 (95% CI: 0.868-0.878) and a precision of 0.890 (95% CI: 0.885-0.895) in the internal validation dataset. In the external validation datasets and one prospective validation dataset, the diagnostic accuracy of GEADS ranged from 0.841 (95% CI: 0.834-0.848) to 0.949 (95% CI: 0.935-0.963). With the help of GEADS, the diagnosing accuracies of novice and expert endoscopists were significantly improved (P < 0.001). CONCLUSIONS: The AI system can assist endoscopists in improving the accuracy of diagnosing upper gastrointestinal diseases.

9.
Front Psychol ; 13: 907929, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35719480

RESUMO

The booming development of educational livestreaming platforms has caused the prevalence of user experience to a certain extent, which profoundly affects users' purchase intention and behavior, and has become a hot topic of current research in the online education field. However, there is a lack of in-depth analysis on the mechanism of the role of user experience in influencing purchase intention. Based on the analysis of user experience and psychological ownership, this study constructs a moderated mediation model to investigate the mediating psychological mechanism and boundary conditions of user experience affecting purchase intention. In this study, a valid sample of 372 users was used for structural equation modeling analysis. The results of the study found that user experience not only had a significant positive effect on purchase intention but was also mediated by psychological ownership. We also found that the effect of psychological ownership on purchase intention was moderated by privacy concerns. This study examines the role of user experience in purchase intention and reveals the mechanism of the role of user experience in an educational livestreaming platform.

10.
Artigo em Inglês | MEDLINE | ID: mdl-35722139

RESUMO

Objective: The aim of the study was to explore the regularity of acupoints in the treatment of amyotrophic lateral sclerosis (ALS) by means of data mining technology. Methods: Nine databases, including SinoMed, Chongqing VIP (CQVIP), China National Knowledge Infrastructure (CNKI), Wanfang Data, Cochrane Library, PubMed, MEDLINE, Web of Science, and Embase, were comprehensively searched till December 2021. The published clinical literature testing acupuncture in the treatment of ALS was eligible for inclusion. Studies were organized to establish the prescription database. Modular data mining analysis, including acupoint frequency, complex network analysis, association rule analysis, and cluster analysis were used to conduct statistical analysis. Results: Forty-two literature studies on 141 acupoints were included, involving 626 times the total application frequency. The top 5 acupoints in application frequency were Hegu (LI 4, 67%), Zusanli (ST 36, 67%), Quchi (LI 11, 52%), Sanyinjiao (SP 6, 48%), and Yanglingquan (GB 34, 45%). The most involved meridian was the large intestine meridian of hand Yangming (90 times). The generally used acupoints were mainly distributed in the lower limbs. The top 5 combinations in application frequency were Hegu-Quchi (75 times), Quchi-Zusanli (66 times), Zusanli-Sanyinjiao (54 times), Hegu-Sanyinjiao (54 times), and Quchi-Sanyinjiao (49 times). The acupoint combinations with the strongest association were Quchi, Hegu, Zusanli, Sanyinjiao, and Shousanli (LI 10). There were 7 acupoint groups according to the cluster analysis. The core prescriptions were Hegu, Zusanli, Quchi, and Jiaji (EX-B 2). Conclusions: Hegu, Zusanli, Quchi, and Jiaji could be used as the main prescriptions in treating ALS. The combination of Quchi, Hegu, Zusanli, and Sanyinjiao should be selected with priority in acupuncture therapy.

11.
Exp Cell Res ; 418(1): 113265, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35716785

RESUMO

Understanding the mechanisms of colorectal cancer (CRC) progression is critical for developing innovative treatment strategies. As an endoplasmic reticulum-located protein, B cell receptor-associated protein 31 (BCAP31) has been identified to be highly expressed in multiple cancers. However, its function and molecular mechanism in CRC remain not fully understood. In the present study, BCAP31 expression and its correlation with the clinical stage were analyzed based on TCGA database. We demonstrated that loss of BCAP31 suppressed CRC cell proliferation in vitro and tumor growth in vivo. Mechanistically, we demonstrated that Emerin was an interaction partner and downstream molecule of BCAP31. Knockdown of BCAP31 promoted the nuclear envelope localization of Emerin, leading to a reduction of ß-catenin accumulation in the nucleus, which resulted in downregulation of Wnt/ß-catenin downstream target genes, including c-Myc, cyclin D1, Survivin, and Mcl-1. Moreover, downregulation of Emerin partially restored the BCAP31 depletion-mediated ß-catenin protein level and tumor suppressive effects in CRC cells.Our data highlights the pivotal role of BCAP31 depletion in inhibiting cell proliferation in CRC cells, and mechanistically via Emerin/ß-catenin signaling, which may serve as a promising target for CRC treatment.

12.
Nat Commun ; 13(1): 3511, 2022 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-35717499

RESUMO

Patchouli (Pogostemon cablin (Blanco) Benth.), a member of the Lamiaceae family, is an important aromatic plant that has been widely used in medicine and perfumery. Here, we report a 1.94 Gb chromosome-scale assembly of the patchouli genome (contig N50 = 7.97 Mb). The gene annotation reveals that tandem duplication of sesquiterpene biosynthetic genes may be a major contributor to the biosynthesis of patchouli bioactivity components. We further phase the genome into two distinct subgenomes (A and B), and identify a chromosome substitution event that have occurred between them. Further investigations show that a burst of universal LTR-RTs in the A subgenome lead to the divergence between two subgenomes. However, no significant subgenome dominance is detected. Finally, we track the evolutionary scenario of patchouli including whole genome tetraploidization, subgenome divergency, hybridization, and chromosome substitution, which are the key forces to determine the complexity of patchouli genome. Our work sheds light on the evolutionary history of patchouli and offers unprecedented genomic resources for fundamental patchouli research and elite germplasm development.


Assuntos
Lamiaceae , Pogostemon , Cromossomos , Haplótipos , Lamiaceae/genética , Pogostemon/genética , Tetraploidia
13.
Zhongguo Zhong Yao Za Zhi ; 47(10): 2614-2622, 2022 May.
Artigo em Chinês | MEDLINE | ID: mdl-35718479

RESUMO

In this study, we used bioinformatic tools to analyze the 3-hydroxy-3-methylglutaryl-CoA reductase(HMGR) genes from Glycyrrhiza uralensis, Artemisia annua, and Arabidopsis thaliana. The results indicated that GuHMGR and AaHMGR contained two transmembrane regions while AtHMGR had three transmembrane regions. GuHMGR, AaHMGR, and AtHMGR all had the active center for catalysis. Three truncated HMGR genes(tHMGRs) of G. uralensi, A. annua, and A. thaliana were respectively ligated to pYES3 vector to construct the recombinant plasmids pYES3-tGuHMGR,pYES3-tAaHMGR,and pYES3-tAtHMGR. Afterwards, the control plasmid pYES3 and the three plasmids and were respectively introduced into Saccharomyces cerevisiae Cen.pk2-1 D, which yielded strains Y0, Y1, Y2, and Y3, respectively. The content of squalene, lanosterol, and ergosterol in these strains was measured by GC-MS. The relative expression of tGuHMGR, tAaHMGR, and tAtHMGR in strains Y1, Y2, and Y3 was determined by quantitative real-time PCR. The results showed that the strain overexpressing tAaHMGR had the highest yield of squalene and the highest total yield of squalene, ergosterol, and lanosterol. The quantitative real-time PCR showed higher relative expression of tAaHMGR than tGuHMGR, consistent with the strain fermentation result. We selected a superior tHMGR by comparing the effects of different tHMGRs on the mevalonate(MVA) pathway flux in S. cerevisiae. The findings can provide a reference for the construction of S. cerevisiae strains with high yields of squalene and terpenoid precursors.


Assuntos
Ácido Mevalônico , Saccharomyces cerevisiae , Ergosterol , Lanosterol , Ácido Mevalônico/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Esqualeno/metabolismo
14.
Genomics ; 114(4): 110400, 2022 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-35691507

RESUMO

Endive (Cichorium endivia L.) is a leafy vegetable in the Asteraceae family. Sesquiterpene lactones (STLs) in endive leaves bring a bitter taste that varies between varieties. Despite their importance in breeding varieties with unique flavours, sesquiterpenoid biosynthesis pathways in endive are poorly understood. We assembled a chromosome-scale endive genome of 641 Mb with a contig N50 of 5.16 Mb and annotated 46,711 protein-coding genes. Several gene families, especially terpene synthases (TPS) genes, expanded significantly in the C. endivia genome. STLs biosynthesis-related genes and TPS genes in more bitter varieties have shown a higher level of expression, which could be attributed to genomic variations. Our results penetrate the origin and diversity of bitter taste and facilitate the molecular breeding of endive varieties with unique bitter tastes. The high-quality endive assembly would provide a reference genome for studying the evolution and diversity of Asteraceae.

15.
J R Soc Interface ; 19(191): 20220298, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35702860

RESUMO

The growth and development of biological tissues and organs strongly depend on the requirements of their multiple functions. Plant veins yield efficient nutrient transport and withstand various external loads. Victoria cruziana, a tropical species of the Nymphaeaceae family of water lilies, has evolved a network of three-dimensional and rugged veins, which yields a superior load-bearing capacity. However, it remains elusive how biological and mechanical factors affect their unique vein layout. In this paper, we propose a multi-functional and large-scale topology optimization method to investigate the morphomechanics of Victoria cruziana veins, which optimizes both the structural stiffness and nutrient transport efficiency. Our results suggest that increasing the branching order of radial veins improves the efficiency of nutrient delivery, and the gradient variation of circumferential vein sizes significantly contributes to the stiffness of the leaf. In the present method, we also consider the optimization of the wall thickness and the maximum layout distance of circumferential veins. Furthermore, biomimetic leaves are fabricated by using the three-dimensional printing technique to verify our theoretical findings. This work not only gains insights into the morphomechanics of Victoria cruziana veins, but also helps the design of, for example, rib-reinforced shells, slabs and dome skeletons.


Assuntos
Nymphaeaceae , Folhas de Planta , Plantas , Suporte de Carga
16.
Appl Bionics Biomech ; 2022: 6775277, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35706509

RESUMO

Objective: This study was designed to evaluate the aspects that affect transfusion following percutaneous nephrolithotomy (PCNL). Patients and Methods. From 2016 to 2019, 665 patients underwent PCNL for the removal of renal calculi at our center (Department of Urology, Shanghai Xu-hui Central Hospital). Complications, including hemorrhages, have been reported. Twenty-three patients (3.5%) have received a blood transfusion, and 12 (1.9%) patients were treated with hyper-selective embolization. We focused on the influencing factors related to postoperative blood transfusion. The factors analyzed were age, sex, hypertension, diabetes, serum creatinine level, preoperative hemoglobin, and the use of anticoagulants or antiplatelet medications; renal and stone factors (i.e., previous surgery, abnormal anatomy, stone side, stone burden, and stone type); and surgical features (i.e., access number, the calyx of puncture, and stone-free rate). These data were analyzed for the presence of bleeding. Results: Among individual factors, preoperative hemoglobin level (p < 0.001) and urinary infections (p < 0.001) were significantly correlated with blood transfusion. Among renal and stone factors, only a history of open surgery was significantly correlated with blood transfusion (p < 0.05). Stone type or stone burden did not correlate with transfusion. Furthermore, no statistically significant correlation was found between surgical features and bleeding, and a lower stone-free rate was reported for the transfusion group. Conclusion: The obtained results demonstrated that PCNL is a safer surgical procedure in a high-volume center; however, anemic conditions, infections, and history of open surgery will significantly increase the transfusion rate following PCNL.

17.
Org Biomol Chem ; 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35703433

RESUMO

Under the catalysis of Pd2(dba)3·CHCl3/(±)-L5 in THF at room temperature, the three-component decarboxylative coupling reactions among alkylidene pyrazolones, allyl carbonates and active methylene compounds proceeded readily and furnished the desired products in acceptable chemical yields. The chemical architecture of the obtained products was unambiguously confirmed by single crystal X-ray analysis.

18.
Signal Transduct Target Ther ; 7(1): 185, 2022 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-35697692

RESUMO

Prolonged activation of nuclear factor (NF)-кB signaling significantly contributes to the development of colorectal cancer (CRC). New therapeutic opportunities are emerging from targeting this distorted cell signaling transduction. Here, we discovered the critical role of RING finger 138 (RNF138) in CRC tumorigenesis through regulating the NF-кB signaling, which is independent of its Ubiquitin-E3 ligase activity involved in DNA damage response. RNF138-/- mice were hyper-susceptible to the switch from colitis to aggressive malignancy, which coincided with sustained aberrant NF-кB signaling in the colonic cells. Furthermore, RNF138 suppresses the activation of NF-кB signaling pathway through preventing the translocation of NIK and IKK-Beta Binding Protein (NIBP) to the cytoplasm, which requires the ubiquitin interaction motif (UIM) domain. More importantly, we uncovered a significant correlation between poor prognosis and the downregulation of RNF138 associated with reinforced NF-кB signaling in clinical settings, raising the possibility of RNF138 dysregulation as an indicator for the therapeutic intervention targeting NF-кB signaling. Using the xenograft models built upon either RNF138-dificient CRC cells or the cells derived from the RNF138-dysregulated CRC patients, we demonstrated that the inhibition of NF-кB signaling effectively hampered tumor growth. Overall, our work defined the pathogenic role of aberrant NF-кB signaling due to RNF138 downregulation in the cascade events from the colitis switch to colonic neoplastic transformation and progression, and also highlights the possibility of targeting the NF-кB signaling in treating specific subtypes of CRC indicated by RNF138-ablation.


Assuntos
Colite , NF-kappa B , Ubiquitina-Proteína Ligases/metabolismo , Animais , Transformação Celular Neoplásica , Colite/genética , Humanos , Camundongos , NF-kappa B/genética , NF-kappa B/metabolismo , Transdução de Sinais/genética , Ubiquitina-Proteína Ligases/genética , Ubiquitinas
19.
J Nurs Manag ; 2022 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-35689407

RESUMO

AIM: To explore the impact of violence and psychological resilience on psychiatric nurses as second victims. BACKGROUND: Workplace violence is a public health concern, seriously influencing medical staff's physical and mental health. However, few pieces of research have concentrated on psychiatric nurses as second victims. METHOD: The sociodemographic data, violence-related data, psychological resilience scale, the Chinese version of the Second Victim Experience and Support Tool were applied to conduct a cross-sectional survey on nurses from psychiatric hospitals. The multiple linear regression model identified significant variables associated with violence-related injury and resilience. RESULTS: A total of 683 nurses completed the survey, of whom 88.3% were women. The average scores of the second victims' experience and support, support, and distress were 3.45 ± 0.43, 3.71 ± 0.57, and 3.19 ± 0.67, respectively. Physical violence, psychological violence, psychological influence, and nursing work environment were positively correlated with the experience and support of the second victims and innocuous violence was a negative factor, which explains 20.6% of the variation. Moreover, physical injury, nursing work environment, resilience restructuring, physical violence, psychological violence, psychological impact, and disagreement about the existence of violence, explained 14.8% of the distress. The physical injury, psychological violence and nursing work environment explained 46.2% of the support. CONCLUSIONS: Our findings suggest that nurses who as second victims after the violence mainly suffer from severe psychological distress and receive the least support for the same. Implication for Nursing Management The study provides clues to help nursing managers emphasis on the distress and support of second victims, provide targeted intervention based on the relevant factors and these results.

20.
Pol J Microbiol ; 71(2): 173-189, 2022 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-35675824

RESUMO

To explore the role of gut microbiota in Graves' disease (GD) and Hashimoto's thyroiditis (HT). Seventy fecal samples were collected, including 27 patients with GD, 27 with HT, and 16 samples from healthy volunteers. Chemiluminescence was used to detect thyroid function and autoantibodies (FT3, FT4, TSH, TRAb, TGAb, and TPOAb); thyroid ultrasound and 16S sequencing were used to analyze the bacteria in fecal samples; KEGG (Kyoto Encyclopedia of Genes and Genomes) and COG (Clusters of Orthologous Groups) were used to analyze the functional prediction and pathogenesis. The overall structure of gut microbiota in the GD and HT groups was significantly different from the healthy control group. Proteobacteria and Actinobacteria contents were the highest in the HT group. Compared to the control group, the GD and HT groups had a higher abundance of Erysipelotrichia, Cyanobacteria, and Ruminococcus_2 and lower levels of Bacillaceae and Megamonas. Further analysis of KEGG found that the "ABC transporter" metabolic pathway was highly correlated with the occurrence of GD and HT. COG analysis showed that the GD and HT groups were enriched in carbohydrate transport and metabolism compared to the healthy control group but not in amino acid transport and metabolism. Our data suggested that Bacillus, Blautia, and Ornithinimicrobium could be used as potential markers to distinguish GD and HT from the healthy population and that "ABC transporter" metabolic pathway may be involved in the pathogenesis of GD and HT.


Assuntos
Microbioma Gastrointestinal , Doença de Graves , Doença de Hashimoto , Autoanticorpos , Fezes , Doença de Graves/diagnóstico , Doença de Graves/patologia , Doença de Hashimoto/diagnóstico , Doença de Hashimoto/patologia , Humanos
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