Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 59
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Kaohsiung J Med Sci ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32052576

RESUMO

It has been reported that tripartite motif containing 26 (TRIM26) is involved in the tumorigenesis of some cancers, but its function in non-small cell lung cancer (NSCLC) is still unclear. In this study, we found that TRIM26 was markedly down-regulated in both of NSCLC tumor tissues and cell lines. Additionally, high expression of TRIM26 in NSCLC patients predicted a positive index for patients' overall survival. What is more, overexpression of TRIM26 significantly suppressed NSCLC cell growth. Our further studies indicated that overexpression of TRIM26 inhibited the phosphorylation of PI3K p85 and AKT. And overexpressed TRIM26 regulated cell cycle-related genes' expression, including downregulating CDK4, Cyclin A, Cyclin D1, Cyclin D3, and Cyclin E, and upregulating p27 expression. Finally, we found that TRIM26 up-regulated PTEN expression by stabilizing PTEN protein in NSCLC cells. Collectively, our present study indicated that TRIM26 was decreased in NSCLC and overexpression of TRIM26 inhibited NSCLC cell growth by suppressing PI3K/AKT pathway, which suggested that TRIM26 could be as a potential target for the treatment of NSCLC in the future.

2.
Aesthetic Plast Surg ; 44(1): 60-69, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31598769

RESUMO

BACKGROUND: In China, traditional preoperative planning of unilateral breast reconstruction mainly depends on anthropometric measurement and visual assessment. Thus, the lack of objective assessment of breast volume and shape would likely result in suboptimal reconstruction outcomes. Three-dimensional surface imaging (3D-SI), which could provide objective measurement data of the breast, may be a promising solution to this problem. METHODS: A retrospective review of patients undergoing tissue expander (TE)/implant breast reconstruction without any mammoplasty surgery on the contralateral sides in our hospital from August 2013 to May 2018 was performed. All the patients underwent unilateral mastectomy with immediate or delayed insertion of TE, followed by an exchange of a silicone gel implant without contralateral procedures. 3D images were obtained at the time of preoperation, the routine expansion visit, and post-exchange of implant. The breast volume measured by 3D-SI served as a guide to conduct the surgery management, such as in deciding the total volume of expansion and guiding the final implant size selection. 3D-SI also provided objective data to evaluate the final outcomes of the reconstruction. RESULTS: Fifty-one patients were included in this study, in which eighteen patients underwent immediate TE insertion and thirty-three patients underwent delayed TE insertion. The ptosis degree of contralateral breasts was assessed as follows: forty-four were normal, and seven showed mild ptosis. The average expansion degree was controlled at 161.6% ± 14.1% compared to the contralateral breasts. The volume of implants exchanged had a strong linear correlation with the 3D volume of the contralateral breasts at the end of expansion (P < 0.01). The mean time of follow-up was 9.1 ± 6.6 months. There was only one patient who experienced TE leakage with secondary infection and received TE exchange. For the immediate reconstruction group, the overall breast symmetry improved at the completion of implant exchange (P < 0.01), with an average asymmetry of 5.3% ± 4.0% compared with 10.6% ± 6.1% initially. For the delayed reconstruction group, the reconstructed side achieved good volume symmetry to the contralateral side (P > 0.05). There was no significant difference in breast basal width between bilateral breasts post-reconstruction (P > 0.05). CONCLUSION: 3D-SI serves as a valuable adjunct by providing accurate 3D volume of breasts within TE/implant breast reconstruction in Chinese patients without obvious breast ptosis, which could facilitate surgeons to achieve good reconstructive outcomes. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

3.
Huan Jing Ke Xue ; 40(11): 4810-4823, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854546

RESUMO

Northeastern China experiences severe atmospheric pollution, with an increasing occurrence of heavy haze episodes. Based on ground monitoring data, satellite products and meteorological products of atmospheric pollutants in northeast China from 2013 to 2017, the characteristics of spatial and temporal distribution of air quality and the causes of heavy haze events in northeast China were discussed. It was found that the "Shenyang-Changchun-Harbin" city belt was the most polluted area in the region on an annual scale. The spatial distribution of air quality index (AQI) values had a clear seasonality, with the worst pollution occurring in winter, an approximately oval-shaped polluted area around western Jilin Province in spring, and the best air quality occurring in summer and most of autumn. The three periods that typically experienced intense haze events were Period I from late-October to early-November (i. e., late autumn and early winter), Period Ⅱ from late-December to January (i. e., the coldest time in winter), and Period Ⅲ from April to mid-May (i. e., spring). During Period I, strong PM2.5 emissions from seasonal crop residue burning and coal burning for winter heating were the dominant reasons for the occurrence of extreme haze events (AQI>300). Period Ⅱ had frequent heavy haze events (200 < AQI < 300) in the coldest months of January and February(200 < AQI < 300), which were due to high PM2.5 emissions from coal burning and vehicle fuel consumption, a lower atmospheric boundary layer, and stagnant atmospheric conditions. Haze events in Period Ⅲ, with high PM10 concentrations, were primarily caused by the regional transportation of windblown dust from degraded grassland in central Inner Mongolia and bare soil in western Jilin Province. Local agricultural tilling could also release PM10 and enhance the levels of windblown dust from tilled soil.

4.
J Sep Sci ; 42(21): 3382-3389, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31503388

RESUMO

"Dogel ebs" was known as Sophora flavescens Ait., a classical traditional Chinese Mongolian herbal medicine, which had the effects on damp-heat dysentery, scrofula, and syndrome of accumulated dampness toxicity. Although the chemical constituents have been clarified by our previous studies, the metabolic transformation of "Dogel ebs" in vivo was still unclear. To explore the mechanism of "Dogel ebs," the metabolites in plasma, bile, and urine samples were investigated. A fast positive and negative ion switching technology was used for the simultaneous determination of flavonoids and alkaloids in "Dogel ebs" in a single run. And a target-group-change coupled with mass defect filtering strategy was utilized to analyze the collected data. 89 parent compounds and 82 metabolites were characterized by high-performance liquid chromatography with quadrupole exactive Orbitrap mass spectrometry. Both phase I and phase II metabolites were observed and the metabolic pathways involved in oxidation, demethylation, acetylation, and glucuronidation. 69 metabolites of "Dogel ebs," including three hydroxyls bonding xanthohumol, formononetin-7-O-glucuronide, 2'-hydroxyl-isoxanthohumol decarboxylation metabolite, oxysophocarpine dehydrogen, 9α-hydroxysophoramine-O-glucuronide, etc. were reported for the first time.

5.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(7): 708-712, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31315773

RESUMO

OBJECTIVE: To construct the recombinant adenoviral vector carrying the rat interleukin-10 (rIL-10) gene, and to investigate whether it is stably expressed in bone marrow mesenchymal stem cells. METHODS: The rIL-10 gene was amplified by PCR from template rIL-10 cDNA, and the recovered 656 bp rIL-10 DNA fragment was cloned into pcDNA3.1 to construct pcDNA3.1-IL-10. Then HEK293 cells were transfected with pcDNA3.1-IL-10 and adenoviral vector for homologous recombination, and sequencing and PCR were used to evaluate whether recombination was successful. HEK293 cells were lysed by repeated freeze-thaw cycles, and bone marrow mesenchymal stem cells were infected with the virus solution containing the rIL-10 gene. Western blot was used to measure the expression of rIL-10 in bone marrow mesenchymal stem cells. RESULTS: Sequencing and PCR verified that the rIL-10 adenoviral vector was successfully constructed, with a virus titer of 4×109 PFU/mL. The expression of IL-10 was detected after bone marrow mesenchymal stem cells were infected by the virus solution containing the rIL-10 gene. CONCLUSIONS: The constructed rIL-10 recombinant adenovirus can mediate the stable expression of rIL-10 gene in bone marrow mesenchymal stem cells, which provides a basis for gene transplantation therapy of inflammatory bowel disease.


Assuntos
Células-Tronco Mesenquimais , Adenoviridae , Animais , Células da Medula Óssea , Vetores Genéticos , Células HEK293 , Humanos , Interleucina-10 , Ratos , Transfecção
6.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(1): 89-93, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30675870

RESUMO

OBJECTIVE: To study the effect of glutamine-supplemented enteral nutrition in regulating the apoptosis of intestinal mucosal cells and promoting mucosal healing in young rats with inflammatory bowl disease (IBD). METHODS: A total of 80 male Sprague-Dawley rats aged 4-5 weeks were randomly divided into 4 groups: blank control, IBD model, short peptide, and short peptide+glutamine (n=20 each). The IBD model was prepared by a single colon perfusion of 3-nitrobenzene sulfonic acid. At 3 days after modeling, the rats in the short peptide group were fed with short peptide formula (100 mL/kg), and those in the short peptide+glutamine group were fed with short peptide formula (100 mL/kg) and glutamine (0.5 g/kg). The course of intervention was 1 week. General conditions were observed after the experiment and their intestinal mucosal tissue was obtained. Hematoxylin-eosin staining was used to observe the pathological change of the intestinal mucosa. RT-PCR was used to measure the expression of apoptosis-regulating genes (bax and bc1-2) and apoptotic signal transduction factors (Caspase-3 and Caspase-9) in the intestinal mucosa. Western blot was used to measure the expression of insulin-like growth factor-1 (IGF-1) in the colonic mucosa. RESULTS: The IBD model group had poorer general conditions than the other three groups (blank control, short peptide and short peptide+glutamine), and the short peptide+glutamine group had better general conditions than the IBD model and short peptide groups. The IBD model group had significantly higher mRNA expression of bax than the other three groups (P<0.05). There was no significant difference in the mRNA expression of bcl-2, Caspase-3 and Caspase-9 among the 4 groups (P>0.05). The short peptide group had a significantly higher level of IGF-1 than the short peptide+glutamine, blank control and IBD model groups (P<0.05). CONCLUSIONS: Glutamine-supplemented enteral nutrition can effectively improve the general nutritional status of young rats with IBD, but it is not better than exclusive enteral nutrition in inhibiting the apoptosis of colonic mucosal cells and stimulating the synthesis of IGF-1 in the intestinal mucosa.


Assuntos
Nutrição Enteral , Animais , Apoptose , Glutamina , Mucosa Intestinal , Masculino , Ratos , Ratos Sprague-Dawley
7.
Front Biosci (Landmark Ed) ; 24: 576-596, 2019 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-30468675

RESUMO

The molecular mechanism of how airway inflammation develops is not fully understood. Withaferin A (WA) is a natural phytochemical isolated from the plant Withania somnifera. It is a well-investigated bioactive compound that possesses a variety of health-promoting effects, including anti-inflammatory and anti-oxidative activities. In the present study, the effect of WA on ovalbumin (OVA)-induced airway inflammation in mice was investigated. The results indicated that pre-treatment with WA inhibited OVA-induced lung injury and fibrosis progression in mice. Furthermore, WA significantly downregulated inflammatory cell infiltration into the bronchoalveolar lavage fluid and significantly reduced pro-inflammatory cytokine expression in the lung tissue specimens. Additionally, WA significantly suppressed transforming growth factor-b1 expression in lung tissues. WA also caused the downregulation of collagen I, collagen III, α-smooth muscle actin and tissue inhibitor of metalloproteinase-1, as well as SMADs and extracellular signal related kinase 1/2 inactivation. Notably, WA significantly reduced the activation of the NLRP3 inflammasome. The results indicate that WA may be an effective novel candidate for the treatment of airway inflammation.


Assuntos
Inflamação/prevenção & controle , Ovalbumina/toxicidade , Sistema Respiratório/efeitos dos fármacos , Vitanolídeos/farmacologia , Animais , Linhagem Celular , Colágeno/genética , Colágeno/metabolismo , Citocinas/genética , Citocinas/metabolismo , Expressão Gênica/efeitos dos fármacos , Humanos , Inflamassomos/efeitos dos fármacos , Inflamassomos/genética , Inflamassomos/metabolismo , Inflamação/genética , Inflamação/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Fibrose Pulmonar/genética , Fibrose Pulmonar/metabolismo , Fibrose Pulmonar/prevenção & controle , Sistema Respiratório/metabolismo , Sistema Respiratório/patologia
8.
J Pediatr Gastroenterol Nutr ; 68(2): 199-206, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30334930

RESUMO

OBJECTIVES: The present study aims to identify the genotype-phenotype correlation in children with Peutz-Jeghers Syndrome (PJS) through the analysis of STK11 gene mutations in the context of clinical and pathological characteristics. METHOD: In this observational cohort study, the clinical characteristics of 18 families diagnosed with pediatric PJS were collected. Genomic DNA from the peripheral blood of affected children and their family members was collected. The coding region of STK11 was amplified by PCR and screened for mutation by Sanger sequencing. The families that were negative for STK11 mutation were further assessed by multiplex ligation-dependent probe amplification (MLPA). RESULT: Initial presentation in affected children was at 1.6 to 14.2 years and included anemia in 8 patients whereas 6 presented for screening by virtue of family history. All patients underwent endoscopy, colonoscopy, and polypectomy. Polyps were distributed throughout the gastrointestinal (GI) tract, including the small intestine, stomach, colon, and rectum.In the 18 pediatric PJS families, STK11 mutations were detected in 8 families by Sanger sequencing, and large deletions were detected in 3 by MLPA, respectively. Nine of the 11 STK11 mutations were de novo, 3 were novel (c.419T>C:p.L140P, c.314T>G:p.L105X), and (c.488_489insACGG p.L164fs). CONCLUSIONS: Although the main clinical features of pediatric PJS were similar to those of PJS cases in adults, a high frequency of STK11 de novo mutations were encountered in our population of patients with PJS.

9.
Cell Physiol Biochem ; 50(4): 1601-1616, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30359966

RESUMO

BACKGROUNDS/AIMS: It has been reported that myocardial infarction (MI) is a risk factor for vascular dementia. However, the molecular mechanism remains largely unknown. METHODS: MI mice were generated by ligation of the left coronary artery (LCA) for 4 weeks. Passive and active avoidance tests were performed to evaluate the cognitive ability of MI mice. A theta-burst stimulation (TBS) protocol was applied to elicit long-term potentiation (LTP) of the perforant pathway-dentate gyrus synapse (PP-DG). Western blot analysis was employed to assess protein levels. RESULTS: In this study, we demonstrated that after 4 weeks of MI, C57BL/6 mice had significantly impaired memory. Compared with the sham group, in vivo physiological recording in the MI group revealed significantly decreased amplitude of population spikes (PS) with no effect on the latency and duration of the stimulus-response curve. The amplitude of LTP was markedly decreased in the MI group compared with the sham group. Further examination showed that the expression of the TBS-LTP-related proteins BDNF, GluA1 and phosphorylated GluA1 were all decreased in the MI group compared with those in the sham group. Strikingly, all these changes were prevented by hippocampal stereotaxic injection of an anti-miR-1 oligonucleotide fragment carried by a lentivirus vector (lenti-pre-AMO-1). CONCLUSION: MI induced cognitive decline and TBS-LTP impairment, and decreased BDNF and GluA1 phosphorylation levels from overexpression of miR-1ated were involved in this process.


Assuntos
Potenciação de Longa Duração/fisiologia , MicroRNAs/metabolismo , Infarto do Miocárdio/patologia , Animais , Antagomirs/metabolismo , Comportamento Animal , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Células Cultivadas , Giro Denteado/fisiologia , Modelos Animais de Doenças , Estimulação Elétrica , Eletrodos Implantados , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Infarto do Miocárdio/metabolismo , Neurônios/citologia , Neurônios/metabolismo , Mapas de Interação de Proteínas , Receptores de AMPA/metabolismo , Sinapses/metabolismo
10.
Acta Cardiol Sin ; 33(6): 567-577, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29167607

RESUMO

Background: This study aimed to evaluate the impact of intra-aortic balloon counterpulsation (IABP) on the prognosis of patients with acute myocardial infarction (AMI). Methods: We identified and included in this study AMI cases treated with IABP from January 1970 to May 2014. For statistical analysis, we utilized RevMan 5.0 software. Results: Fourteen RCTs with a total population of 2538 were included in this study. The in-hospital and 30-day mortality rate in the IABP group was not significantly lower than those in the non-IABP group. Subgroup analysis according to the type of revascularization, OR values of TT subgroup, PCI subgroup, and CABG subgroup were 0.64 (95% CI 0.25-1.61, p = 0.34), 0.85 (95% CI 0.65-1.11, p = 0.23) and 0.46 (95% CI 0.13-1.63, p = 0.23). And OR values of AMI patients in the before and after PCI subgroup were 0.43 (95% CI 0.21-0.91, p = 0.03) and 1.36 (95% CI 0.76-2.41, p = 0.30). The 6-month mortality in the IABP group was not significantly lower than that in the non-IABP group. And OR values of 6-month mortalities of the before and after PCI subgroup were 0.47 (95% CI 0.26-0.86, p = 0.01) and 1.40 (95% CI 0.57-3.45, p = 0.47). Conclusions: IABP did not reduce the in-hospital and 30-day mortality of AMI patients, and did not reduce the 6-month mortality. But IABP used in AMI patients before PCI was associated not only with reduced in-hospital and 30-day mortality, but also reduced 6-month mortality.

11.
Oncotarget ; 8(41): 70214-70225, 2017 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-29050273

RESUMO

Colorectal cancer (CRC) is one of the most common cancers, but the mechanisms underlying its initiation and progression are largely unknown. TGIF1 (TGFB induced factor homeobox 1) is a transcriptional corepressor that belongs to the three-amino acid loop extension (TALE) superclass of atypical homeodomains. It has been reported that TGIF1 is highly expressed in mammary cancer and non-small cell lung cancer and can enhance tumor progression. However, the role of TGIF1 in colorectal cancer remains unknown. Here, we report that TGIF1 is significantly upregulated in colorectal cancers, and its high expression predicts poor prognosis. Overexpression of TGIF1 markedly promotes the proliferation of colorectal cancer cells both in vivo and in vitro. In addition, TGIF1 activates Wnt/ß-catenin signaling, and the homeodomain is indispensable for Wnt activation and ß-catenin interaction. Taken together, our results suggest that TGIF1 is a novel colorectal tumor promoter and indicate that TGIF1 enhances colorectal cancer tumorigenesis through activating Wnt signaling.

12.
Sci Total Environ ; 601-602: 784-792, 2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-28578236

RESUMO

Photodegradation due to litter exposure to solar UV radiation is presumed to contribute to the surprisingly fast decomposition in some arid and semi-arid regions; however, few studies have directly examined photodegradation effects in hyper-arid regions (annual precipitation <150mm) and its dependence on precipitation. Three litters with different initial qualities (low vs high C:N) were decomposed under full spectrum sunlight (UV radiation) and UV filtering from solar radiation at three sites with contrasting precipitation amounts (144mm, 76mm and 16mm) for 2.5years. UV radiation increased mass loss and litter decomposition rates by 23-70%. UV photodegradation effects (UVE) on litter decomposition rate differed among experimental sites, with significantly stronger effects in less arid sites (144mm and 76mm) than more arid site (16mm). High-quality litter (low C:N ratio) showed the fastest decomposition rate, and UVE was also affected by litter quality, but no consistent trend was observed. Litter N loss was greatest in full sunlight and the linear relationships between C and N contents was not changed by UV filtering over time. UV radiation increased C loss of all fractions, and hemicellulose and cell solubles showed significant contributions to litter mass loss. Our findings suggest that UV photodegradation can increase mass loss and nutrient release by the positive priming effects on microbial decomposition in hyper-arid regions, although UVE differed among three sites with contrasting precipitation amounts.

13.
Cell Death Dis ; 8(6): e2850, 2017 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-28569780

RESUMO

Impaired synaptic plasticity and neuron loss are hallmarks of Alzheimer's disease and vascular dementia. Here, we found that chronic brain hypoperfusion (CBH) by bilateral common carotid artery occlusion (2VO) decreased the total length, numbers and crossings of dendrites and caused neuron death in rat hippocampi and cortices. It also led to increase in N-terminal ß-amyloid precursor protein (N-APP) and death receptor-6 (DR6) protein levels and in the activation of caspase-3 and caspase-6. Further study showed that DR6 protein was downregulated by miR-195 overexpression, upregulated by miR-195 inhibition, and unchanged by binding-site mutation and miR-masks. Knockdown of endogenous miR-195 by lentiviral vector-mediated overexpression of its antisense molecule (lenti-pre-AMO-miR-195) decreased the total length, numbers and crossings of dendrites and neuron death, upregulated N-APP and DR6 levels, and elevated cleaved caspase-3 and caspase-6 levels. Overexpression of miR-195 using lenti-pre-miR-195 prevented these changes triggered by 2VO. We conclude that miR-195 is involved in CBH-induced dendritic degeneration and neuron death through activation of the N-APP/DR6/caspase pathway.


Assuntos
Precursor de Proteína beta-Amiloide/genética , Isquemia Encefálica/genética , MicroRNAs/genética , Neurônios/metabolismo , Receptores de Morte Celular/genética , Precursor de Proteína beta-Amiloide/metabolismo , Animais , Sequência de Bases , Sítios de Ligação , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patologia , Região CA1 Hipocampal/metabolismo , Região CA1 Hipocampal/patologia , Artérias Carótidas/cirurgia , Caspase 3/genética , Caspase 3/metabolismo , Caspase 6/genética , Caspase 6/metabolismo , Morte Celular , Córtex Cerebral/metabolismo , Córtex Cerebral/patologia , Transtornos Cerebrovasculares/patologia , Transtornos Cerebrovasculares/cirurgia , Giro Denteado/metabolismo , Giro Denteado/patologia , Regulação da Expressão Gênica , Masculino , MicroRNAs/antagonistas & inibidores , MicroRNAs/metabolismo , Neurônios/patologia , Oligorribonucleotídeos Antissenso/genética , Oligorribonucleotídeos Antissenso/metabolismo , Cultura Primária de Células , Ratos , Ratos Sprague-Dawley , Receptores de Morte Celular/metabolismo , Transdução de Sinais
14.
Guang Pu Xue Yu Guang Pu Fen Xi ; 37(3): 733-6, 2017 Mar.
Artigo em Chinês, Inglês | MEDLINE | ID: mdl-30148586

RESUMO

A total of two hundred cases of surface Fourier transform infrared (FTIR) spectra from normal female breast were obtained by scanning two hundred enrolled healthy volunteers' upper limit of the right breast outside the skin with two mid-infrared optical fibers and an attenuated total reflectance (ATR) probe. Twelve characteristic bands of the above FTIR spectra, related to deoxyribonucleic acid, ribonucleic acid, proteins, lipids and carbohydrate were analyzed. Thirty-six spectral parameters of these characteristic bands, including peak positions (P), intensities (I) and full width at half maximum (F) of FTIR spectrums, were statistically analyzed. Meantime, 90% normal reference value range, mean value, standard deviation of above characteristic parameters was obtained and calculated. In present study, we had establish the normal reference range of spectral parameters of characteristic bands in the surface Fourier transform infrared spectra scanned from normal female breast, for the first time. Meanwhile, for further implementation of Fourier transform infrared spectroscopy technique in noninvasive, fast and efficient characteristic clinical diagnosis of breast benign and malignant diseases, these research results was of great importance. Eventually, this study also had made an attempt to explore the profound theoretical basis in the biomedical application of Fourier transform infrared spectroscopy technique.


Assuntos
Doenças Mamárias/diagnóstico por imagem , Mama/diagnóstico por imagem , Espectroscopia de Infravermelho com Transformada de Fourier , Carboidratos , Feminino , Análise de Fourier , Humanos , Lipídeos
15.
Ying Yong Sheng Tai Xue Bao ; 27(1): 117-24, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-27228600

RESUMO

Field experiments were carried out to study the effects of different mulching times (30 or 60 d after previous wheat harvest) and mulching methods (whole-mulching, half-mulching and no- mulching) on wheat plant N absorption and assimilation after deep plowing in fallow period on dryland. The results showed that mulching improved water storage efficiency in fallow period and soil water storage of 0-300 cm at sowing stage significantly. Mulching in fallow period, especially the whole-mulching, increased the N accumulation amount of each growth stage, N translocation amount and rate before anthesis (NABA) , and N accumulation amount after anthesis (NAAA) and grain N. Yield, N uptake efficiency (NUPE), N partial factor productivity (NPFP) and N harvest index (NHI) were all significantly increased under mulching, and whole-mulching had better effects. Mulching at 30 d after previous wheat harvest significantly increased the soil water storage of 0-300 cm at sowing stage and the water storage efficiency in fallow period compared with mulching at 60 d after previous wheat harvest. Compared with mulching at 60 d after previous wheat harvest, mulching at 30 d increased the N accumulation amount of each growth stage, the N accumulation amount in leaf and shoot at maturity, the NABA of stem + sheath, leaf and shoot and the yield. The soil water at the depth of 0-300 cm at sowing stage was positively correlated to N mobilization amount before anthesis and N accumulation amount after anthesis. The N mobilization amount of stem + sheath had a remarkable direct effect on its yield with the direct path coefficient of 0.619. In summary, mulching in fallow period increased the soil moisture at sowing stage to promote N absorption and utilization, increase yield and improve quality, and whole-mulching in advance had better effects.


Assuntos
Agricultura/métodos , Conservação dos Recursos Naturais/métodos , Nitrogênio/análise , Triticum , Água , Grão Comestível , Folhas de Planta , Caules de Planta , Solo
16.
Neurol India ; 63(4): 631-3, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26238915
17.
Australas J Dermatol ; 56(3): 202-5, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25817568

RESUMO

Raynaud's phenomenon is often accompanied by pain, digital ulceration and compromised daily activities. Pharmacological therapy or sympathectomies have been administered to diminish these symptoms but existing treatments are not invariably efficacious. A recent case series has described the use of botulinum toxin type A in the treatment of Raynaud's phenomenon. We report two patients with severe or mild Raynaud's phenomenon who were injected with BTX-A; both of whom experienced clinical and image improvement after treatment.


Assuntos
Inibidores da Liberação da Acetilcolina/uso terapêutico , Toxinas Botulínicas Tipo A/uso terapêutico , Doença de Raynaud/tratamento farmacológico , Adulto , Feminino , Humanos , Angiografia por Ressonância Magnética , Doença de Raynaud/diagnóstico por imagem
18.
J Thromb Thrombolysis ; 40(2): 156-60, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25381164

RESUMO

The objective of this study was to review all cases in literature in which the clinical manifestations of ischemic stroke and immune thrombocytopenia (ITP) were presented in the same patient including a new case of our own and discuss the possible mechanism and management of this syndrome. We reviewed 12 reports in which 18 cases were diagnosed as ischemic stroke and ITP. The clinical manifestations and ischemic lesion patterns of the 18 cases and our new case were analyzed in detail to elucidate the characteristics and management of this kind of syndrome. Of all the cases, 8 females and 10 males, 10 of them were Koreans; 3 were Americans; 3 were Japanese; 1 was British and 1 was Australian. The age of eight patients was no more than 50 years old. Most of them had a low platelet count. CT and/or MRI of brain were seen in all tested cases. Prognosis of ischemic stroke was good in 18 of the 19 patients. Although extremely rare, ischemic stroke and ITP may present in the same patient with variant characteristics. This paradoxical mechanism and management of ischemic stroke associated with ITP requires further investigation.


Assuntos
Isquemia Encefálica , Púrpura Trombocitopênica Idiopática , Acidente Vascular Cerebral , Adulto , Isquemia Encefálica/etiologia , Isquemia Encefálica/patologia , Isquemia Encefálica/terapia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Púrpura Trombocitopênica Idiopática/complicações , Púrpura Trombocitopênica Idiopática/patologia , Púrpura Trombocitopênica Idiopática/terapia , Acidente Vascular Cerebral/etiologia , Acidente Vascular Cerebral/patologia , Acidente Vascular Cerebral/terapia
19.
Guang Pu Xue Yu Guang Pu Fen Xi ; 34(7): 1736-41, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25269271

RESUMO

Land surface emissivity (LSE) has already been recognized as a crucial parameter for the determination of land surface temperature (LST). There is an ill-posed problem for the retrieval of LST and LSE. And laboratory-based emissivity is measured in natural constant conditions, which is limited in the application in thermal remote sensing. To solve the above problems, the coupling of LST and LSE is explored to eliminate temperature effects and improve the accuracy of LES. And then, the estimation accuracy of LST from passive remote sensing images will be improved. For different land surface materials, the coupling of land surface emissivity and land surface temperature is various. This paper focuses on studying concrete surface that is one of the typical man-made materials in urban. First the experiments of measuring concrete surface emissivity and concrete surface temperature in natural conditions are arranged reasonably and the suitable data are selected under ideal atmosphere conductions. Then to improve the determination accuracy of concrete surface emissivity, the algorithm worked on the computer of Fourier Transform Infrared Spectroradiometer (FTIR) has been improved by the most adapted temperature and emissivity separation algorithm. Finally the coupling of concrete surface temperature and concrete surface emissivity is analyzed and the coupling model of concrete surface temperature and concrete surface emissivity is established. The results show that there is a highest correlation coefficient between the second derivative of emissivity spectra and concrete surface temperature, and the correlation coefficient is -0.925 1. The best coupling model is the stepwise regression model, whose determination coefficient (R2) is 0.886. The determination coefficient (R2) is 0.905 and the root mean squares error (RMSE) is 0.292 1 in the validation of the model. The coupling model of concrete surface temperature and concrete surface emissivity under natural conditions provides a new approach for improving the estimation accuracy of LST from passive remote sensing images.

20.
Zhongguo Dang Dai Er Ke Za Zhi ; 16(9): 914-8, 2014 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-25229959

RESUMO

OBJECTIVE: To study the clinical characteristics of hospitalized infants with allergic proctocolitis, and to provide a scientific basis for early diagnosis and effective treatment of allergic proctocolitis. METHODS: The clinical data of 96 infants with allergic proctocolitis between September 2011 and March 2013 were reviewed retrospectively. Descriptive analysis was performed to assess the clinical characteristics of allergic proctocolitis. RESULTS: The most common clinical manifestation was diarrhea in the 96 infants. The electronic colonoscopy results indicated that 40% of the infants had multiple small nodules, 26% showed focal erythema and brittle mucous membranes, 25% showed multiple superficial erosion, and 9% showed ulcers with surface exudates. The affected areas included the sigmoid colon (87%), rectum (24%), descending colon (13%), and transverse colon ascending colon and ileocecal junction (8%). Histopathologic examination showed eosinophilic infiltration of mucosal layers, the condition of which was mild to moderate in 89% and severe and extremely severe in 12% of the infants. To treat the allergic proctocolitis, mothers and infants were suggested to avoid allergenic foods; 43% of them continued breastfeeding, 45% switched to highly hydrolyzed protein formula, and 13% were prescribed amino acid-based elemental formula. All infants were in complete remission at discharge. CONCLUSIONS: As the clinical manifestations of allergic proctocolitis in infants lack specificity, the electronic colonoscopy and mucosal histopathologic examination are helpful for early and differential diagnosis. The best treatment is to avoid allergenic foods. Formula-feeding infants should be prescribed highly hydrolyzed protein formula or amino acid-based elemental formula.


Assuntos
Proctocolite/patologia , Colonoscopia , Feminino , Humanos , Lactente , Masculino , Proctocolite/diagnóstico , Proctocolite/terapia , Estudos Retrospectivos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA