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1.
Artigo em Inglês | MEDLINE | ID: mdl-33938405

RESUMO

An efficient and simple method for determining vanillin, methyl vanillin and ethyl vanillin in milk and dairy products was developed using a liquid-liquid extraction (LLE) procedure coupled to high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Different extraction procedures were tested and optimised by spiking three vanillin compounds into a blank matrix in which none of any food additives were detected, and the extraction with acetonitrile solution and n-hexane as cleaning sorbent allowed an efficient recovery of 87.6-101.7% with RSDs less than 5%. The limit of detection (LOD) ranged from 6.2 to 20.1 µg/kg. High sensitivity, accuracy and selectivity were found for the in-house validated method, which can eliminate the interferences from complicated matrices effectively, and fulfil the quality criteria for routine laboratory application for real samples. The developed method was then finally applied to screen the three analytes in 65 milk and dairy products including infant formula milk powders from local markets to check for compliance with Chinese Regulation. Concentrations of the total vanillin and ethyl vanillin ranged from 0.0323 to 246.3 mg/kg, which is within the limits of Chinese regulations.

2.
Biosens Bioelectron ; 184: 113220, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33878592

RESUMO

A hybrid recognition interface combining peptide and molecularly imprinted polymer (MIP) was achieved by introducing a vancomycin binding tripeptide in the preparation of MIP to implement high affinity and specificity recognition of vancomycin in complex matrices. The tripeptide that can specifically bind vancomycin was immobilized onto gold nanoparticles (GNPs) deposited on a glassy carbon electrode (GCE) by Au-S bond, and then a controlled electropolymerization of dopamine was carried out to imprint the vancomycin-peptide complex. After removing vancomycin from the polydopamine (PDA), hybrid peptide-MIP cavities containing multiple binding sites for vancomycin in the MIPDA/peptide/GNPs/GCE were obtained. The electrode had better selectivity and higher sensitivity toward vancomycin than either peptide or MIP modified GNPs/GCE, and the limit of quantification was as low as 10 pM by electrochemical impedance spectroscopy. The real samples, including fetal calf serum, probiotic drink and honey spiked with 0.17-2.0 µM vancomycin were analyzed on the MIPDA/peptide/GNPs/GCE, with the recoveries of 92.16-104.67%. The present study provides a sensitive, reliable method for the detection of vancomycin in complex matrices.

3.
J Biomater Appl ; 35(10): 1372-1379, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33563082

RESUMO

Curcumin (Cur) is an active ingredient of Curcuma longa. Cur has many pharmacological effects, such as anti-inflammation, anti-oxidation, anticoagulation, hypolipidemic, anti-angiogenesis and anti-cancer. An injectable curcumin loaded chitosan/hydroxyapatite bone cement (Cur-CS/HA) was prepared as a bone scaffold and drug delivery. Tween 20, a nonionic surfactant, was incorporated into the cement to improve the solubility of curcumin. Four types of Cur-CS/HA (Group0, Group1, Group5 and Group10) were prepared with different Tween 20 ratios (0, 1, 5 and 10%, respectively). The samples were characterized by infrared spectroscopy (IR), X-ray diffraction (XRD) and scanning electron microscope (SEM). Compression tests were carried out to evaluate the strength of the scaffolds. In addition, the inhibition assay was carried out on MG63 cells with the extracts of drug loaded materials. The results showed that Cur had an effect on the setting time (p < 0.05). Cur reduced the compressive strength of the CS/HA cement (p < 0.05). The release studies showed that Tween 20 could effectively improve the solubility of curcumin. When the Tween 20 content in cement increased from 0 to 10%, the cumulative release (30 d) of Cur increased from 5.5 to 10.6%. Moreover, the cement had good injectability, good anti-collapsibility and good biocompatibility to meet the clinical requirements. The result of inhibition assay showed that Cur-CS/HA could inhibit the proliferation of MG63 cells. Tween 20 incorporated Cur-CS/HA had great potential to use as a drug-loaded artificial bone material.

4.
Chem Commun (Camb) ; 57(10): 1238-1241, 2021 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-33416822

RESUMO

A porous hafnium-phosphonate MOF was synthesized using imidazole ionic liquids (ILs), namely PHOS-100(Hf), which has exceptional chemical stability in aqueous environments, even fuming acids. Its rigid framework with permanent porosity makes PHOS-100 an ideal candidate as a platform to fill with different functional guests such as acidic HCl, H2SO4, or H3PO4. The as-synthesized ILs@PHOS-100 exhibits significant humidity-dependent proton conductivities, increasing by four orders of magnitude from 45% RH to 95% RH at 25 °C. After post-treatment with strong acids, the acids@PHOS-100 show enhanced proton conduction at low relative humidities.

5.
Life Sci ; 270: 119088, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33482188

RESUMO

This study aimed to determine whether MG-132 as a proteasome inhibitor can effectively hinder pterygium progression, and to screen out potential regulators involved in MG-132 mediated process. Human pterygium fibroblasts (HPFs) were derived from pterygium tissues from 5 patients. Cell proliferation was examined by MTT, cell cycle and apoptosis were detected by flow cytometry. The overgrowth pterygium tissues were characterized by H&E staining and IHC compared with normal tissues. Differential mRNA expression with MG-132 treatment was determined by RNA sequencing and analyzed by GO and KEGG pathways. The expression levels of Nrf2, MCPIP1, CDKN1B and XBP1, four genes closely associated with pterygium, were detected by RT-qPCR and western blotting. MG-132 dose-dependently inhibited the growth of HPFs, induced G2/M phase arrest of cell cycle at a certain dose, and also caused cell apoptosis, with the levels of cleaved caspase3, cleaved PARP, Bax and p21 increased. Ki-67 and Bcl-2 were highly expressed while Bax was decreased in pterygium tissues. Total 7199 differentially expressed genes (DEGs) were identified, including HSPA family most significantly increased, and AL590428.1, AL122125.1 and lincRNAs such as FGF14-AS2 decreased. The up-regulated DEGs were mainly enriched in RNA degradation pathway, while down-regulated DEGs were related to the regulation of cell cycle. The expressions of Nrf2 and MCPIP1 were significantly increased, while XBP1 and CDKN1B were decreased. In conclusion, MG-132 inhibited the proliferation and induced apoptosis of HPFs in vitro with 7199 DEGs participated in, which may provide a useful reference for the exploitation of MG-132 in treating pterygium.


Assuntos
Leupeptinas/farmacologia , Pterígio/genética , Pterígio/metabolismo , Adulto , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , China , Túnica Conjuntiva/metabolismo , Feminino , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Humanos , Leupeptinas/metabolismo , Masculino , Pessoa de Meia-Idade , Cultura Primária de Células , Inibidores de Proteassoma/farmacologia , Pterígio/tratamento farmacológico
6.
Methods Mol Biol ; 2251: 195-204, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33481241

RESUMO

A large proportion of proteins are expected to interact with cellular membranes to carry out their physiological functions in processes such as membrane transport, morphogenesis, cytoskeletal organization, and signal transduction. The recruitment of proteins at the membrane-cytoplasm interface and their activities are precisely regulated by phosphoinositides, which are negatively charged phospholipids found on the cytoplasmic leaflet of cellular membranes and play critical roles in membrane homeostasis and cellular signaling. Thus, it is important to reveal which proteins interact with phosphoinositides and to elucidate the underlying mechanisms. Here, we present two standard in vitro methods, liposome co-sedimentation and co-flotation assays, to study lipid-protein interactions. Liposomes can mimic various biological membranes in these assays because their lipid compositions and concentrations can be varied. Thus, in addition to mechanisms of lipid-protein interactions, these methods provide information on the possible specificities of proteins toward certain lipids such as specific phosphoinositide species and can hence shed light on the roles of membrane interactions on the functions of membrane-associated proteins.


Assuntos
Lipossomos/análise , Fosfatidilinositóis/análise , Mapeamento de Interação de Proteínas/métodos , Membrana Celular/metabolismo , Humanos , Lipossomos/metabolismo , Proteínas de Membrana/metabolismo , Membranas/metabolismo , Fosfatidilinositóis/metabolismo , Fosfolipídeos/metabolismo , Fosforilação , Ligação Proteica/fisiologia , Domínios Proteicos/fisiologia , Proteínas/química , Transdução de Sinais/fisiologia
7.
Methods Mol Biol ; 2251: 215-223, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33481243

RESUMO

Phosphoinositides play important roles in the regulation of protein recruitment at specialized membrane domains, protein activity, and membrane dynamics. Phosphoinositide-protein interplay occurs via multiple mechanisms and proteins associate with membranes through different binding patterns. Determinations of membrane-binding mode and membrane penetration depth of proteins in lipid bilayer are thus important steps in characterizing the molecular mechanisms of membrane-protein interactions. Here, we show two standard in vitro assays using liposomes, diphenylhexatriene (DPH) anisotropy, and fluorescence quenching by brominated lipids to determine membrane penetration of proteins into lipid bilayer. These methods will provide useful tools to study membrane-protein association and uncover molecular details of protein-lipid interplay, which are important for understanding biological functions of membrane-associated proteins and membrane dynamics.


Assuntos
Polarização de Fluorescência/métodos , Fluidez de Membrana/fisiologia , Espectrometria de Fluorescência/métodos , Animais , Difenilexatrieno/química , Fluorescência , Corantes Fluorescentes/química , Humanos , Bicamadas Lipídicas/química , Lipossomos/química , Proteínas de Membrana/análise , Proteínas de Membrana/química , Membranas/química , Fosfatidilcolinas/química , Fosfatidilinositóis/análise , Fosfatidilinositóis/química
8.
J Hazard Mater ; 401: 123410, 2021 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-32653798

RESUMO

Despite organophosphate esters (OPEs) are ubiquitous in the environment, limited information is available about their tissue-specific accumulation potential in marine fish and exposure risks. Ten fish species from the coastal area of Laizhou Bay, North China, were sampled and analyzed to investigate tissue levels, bioaccumulation, and human exposure risks of 20 OPEs. Seventeen OPEs were detected in fish tissues with total concentration ranging from 6.6-107 ng/g dry weight. The average log bioaccumulation factor (BAF) values of OPEs ranged from 2.8 to 4.4 in livers, 2.3-3.8 in muscles, 2.5-3.9 in gills, and 2.8-4.4 in kidneys. The log BAF values of OPEs significantly increased with increasing their log KOW values (r = 0.55-0.63, p < 0.001). The estimated daily intake of OPEs ranged from 1.7-12.0 and 3.1-22.1 ng/kg bw/d for rural and urban residents, respectively. The hazard quotients of OPEs were in the range of 4 × 10 -5 to 6.7 × 10 -4 and 7 × 10 -5 to 1.2 × 10 -3 for rural and urban residents, respectively. Results showed that the human health risks of OPEs associated with fish consumption is at low level.

9.
Front Genet ; 11: 587509, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33193724

RESUMO

Chinese sacbrood virus (CSBV) is a serious threat to eastern honeybees (Apis cerana), especially larvae. However, the pathological mechanism of this deadly disease remains unclear. Here, we employed mRNA and small RNA (sRNA) transcriptome approach to investigate the microRNAs (miRNAs) and small interfering RNAs (siRNAs) expression changes of A. cerana larvae infected with CSBV under natural condition. We found that serine proteases involved in immune response were down-regulated, while the expression of siRNAs targeted to serine proteases were up-regulated. In addition, CSBV infection also affected the expression of larvae cuticle proteins such as larval cuticle proteins A1A and A3A, resulting in increased susceptibility to CSBV infection. Together, our results provide insights into sRNAs that they are likely to be involved in regulating honeybee immune response.

10.
J Mech Behav Biomed Mater ; : 104169, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33160912

RESUMO

Coating and cross-linking have been widely used to improve the properties of materials in tissue engineering. A chitosan/hydroxyapatite (CS/HA) comby scaffold with high porosity was prepared via a 3D printed pore-forming mold. The scaffold was then treated with gelatin (Gel) coating and was cross-linked by glutaraldehyde (GA) in order to improve the mechanical strength. The materials were characterized by infrared spectroscopy (IR) and X-ray diffraction (XRD). The structure of the scaffolds was observed by Scanning Electron Microscopy (SEM). Compression tests were carried out to evaluate the strength of the scaffolds. The behaviors and responses of preosteoblast cells on the scaffolds were studied as well. The results showed that gelatin coating and cross-linking significantly enhanced the mechanical strength of the porous scaffolds. Cell culture experiment indicated that the scaffold had good cytocompatibility. The combined application of 3DP structure construction and biopolymer coating/cross-linking would offer some new ideas in fabrication of porous scaffolds with enhanced strength and good biocompatibility for tissue engineering.

11.
Sci Total Environ ; : 142891, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33109368

RESUMO

Wetlands have attracted much attention due to releases of organophosphate esters (OPEs) and other emerging contaminants into this particular environment. Here, Suaeda salsa plants and wetland soils collected from Laizhou Bay, North China, were analyzed to investigate the levels, distribution, and soil-plant transfer of OPEs in these ecosystems. The Σ18OPEs concentrations ranged from 137 to 386 ng/g dry weight (dw), whereas in rhizosphere the concentrations were between 99.8 and 198 ng/g dw. Suaeda salsa rhizosphere could promote the absorption of OPEs in wetlands, and Suaeda salsa root presents a greater rate of absorption. The Σ18OPEs concentrations ranged from 32.9 to 56.8 ng/g dw in roots, 3.93 to 7.51 ng/g dw in stems, and 2.79 to 4.06 ng/g dw in leaves. Log RCFs, log TFr-s and log TFs-l showed no significant correlations with their log KOW, indicating the complexity of uptake and translocation in the natural environment. Predictive model for the OPEs availability to Suaeda salsa was established from the experimental data. The field-based BCFs of all OPEs were dependent on KOW, decreased with increasing KOW. This study provides important insights into the phytoremediation potential of OPEs using Suaeda salsa as an effective strategy and their role in environmental risk assessment of OPEs in wetlands.

12.
Oncogene ; 39(44): 6802-6815, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32973332

RESUMO

Snail is a master inducer of epithelial-mesenchymal transition (EMT) and metastasis, however, Snail protein is labile and is quickly degraded through the predominate ubiquitination-mediated proteasome pathway. Deubiquitinases (DUBs) can counteract the Snail degradation process to maintain high level of Snail protein in cancer cells. In this study, we screened a cDNA library containing 79 DUBs, and discovered that a panel of DUBs consisting of USP13, USP28, USP29, USP37, OTUD6A, and DUB3 can markedly stabilize Snail protein, with USP29 displaying the strongest activity to prevent Snail degradation. Mechanistically, USP29 enhances the interaction of Snail and SCP1, resulting in simultaneous dephosphorylation and deubiquitination of Snail and thereafter cooperative prevention of Snail degradation. Biologically, ectopic expression of USP29 promotes gastric cancer cell migration, and depletion of Snail abolishes USP29-mediated cell migration; and USP29 can be induced by major EMT and metastatic inducing factors such as TGFß, TNFα, and hypoxia. More importantly, high expression levels of Snail, USP29, and SCP1 are associated with poor survival and prognosis. Collectively, these data indicate that Snail is a crucial substrate for USP29 to promote cell migration and USP29/SCP1 complex may be new therapeutic targets to treat metastatic cancer.

13.
Front Pharmacol ; 11: 1299, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32973524

RESUMO

Background: Nephrolithiasis is a systemic metabolic disease with a high prevalence worldwide and is closely related to lipid-mediated oxidative stress and inflammation. Orthosiphon stamineus Benth. (OS) is a traditional medicinal herb mainly containing flavonoids, caffeic acid derivatives, and terpenoids, which has the effect of treating urinary stones. However, the active ingredients of OS for the treatment of kidney stones and their regulatory mechanisms remain unknown. As a powerful antioxidant, flavonoids from herbs can mitigate calcium oxalate stone formation by scavenging radical. Thus, this work focused on EtOAc extract of OS (EEOS, mainly flavonoids) and aimed to reveal the potential intrinsic mechanism of EEOS in the treatment of kidney stones disease. Methods: Firstly, 75% ethanol extract of OS was further extracted with EtOAc to obtain EtOAc extract containing 88.82% flavonoids. Secondly, the extract was subjected to component analysis and used in animal experiments. Then, an untargeted lipidomics based on ultrahigh performance liquid chromatography coupled with TripleTOF 5600 mass spectrometer (UPLC-QTOF-MS) was performed to test the lipid changes of kidneys in the control group, model group and EEOS treatment groups. Finally, multivariate statistical analysis was used to identify differences between the lipid profiles of mice in the model group and the EEOS group. Results: Fifty-one lipid metabolites were significantly different between the mice in the model group and the EEOS intervention group, including glycerophosphocholines, glycerophosphoethanolamines, glycerophosphoinositols, and glycerophosphoglycerols. And the composition of glycerophospholipids-esterified ω-3 polyunsaturated fatty acids and glycerophospholipid subclasses in the kidneys of the EEOS group significantly changed compared to model group. Conclusions: The EEOS can inhibit the stones formation by improving oxidative stress and inflammation mediated by glycerophospholipid metabolism. This study reveals the potential mechanism of EEOS for kidney stones treatment at the lipid molecule level, providing a new direction for further study of the efficacy of OS.

14.
Insects ; 11(9)2020 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-32932828

RESUMO

Varroa destructor is by far the most serious threat to the western honey bee, Apis mellifera. A screen bottom board, a cultural method for mite control, is a modified bottom board with a screen that allows mites to fall onto a sticky board, or the grass or soil below the screen. Whether or not a screen bottom board can reduce varroa significantly has been controversial. Most studies show a trend of lower varroa populations in colonies with these boards, but the results are usually not statistically significant. To understand whether the negative results have been due to small sample sizes, or because the board is actually ineffective, we conducted a meta-analysis with seven published studies with a total of 145 colonies. Meta-analysis showed that the confidence intervals of the combined effect sizes were negative with a Hedges' g of -1.09 (SE 0.39, 95% CI -2.0 to -0.19, p < 0.01), which suggests that the varroa population in colonies with screen bottom boards is significantly lower compared to those with traditional wooden floors. We thus conclude that the screen bottom board does have a significantly negative impact on the varroa population and can be part of tool kits for mite control.

15.
Environ Sci Process Impacts ; 22(9): 1842-1851, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32966458

RESUMO

This study investigates the formation of persistent free radicals (PFRs) on particulate organic matter (POM) under irradiation in water. A montmorillonite-humic acid complex (Mnt-HA complex) was prepared to simulate POM, and the generated PFRs were detected by the electron paramagnetic resonance (EPR) technique. EPR signals with the trend of an initial increase and then a decrease were observed under irradiation for 8 days, and the g factors were in the range of 2.0034-2.0039, which indicated the generation of carbon-centered radicals with electrophilic moieties. Different concentrations and types of halophenols and transition-metal ions were respectively adsorbed on the Mnt-HA complex to probe their influence on the formation of PFRs. The amount of PFRs generated in the Mnt-HA complexes was in the order: 2-bromophenol (2-BP) > 2,4-dibromophenol (2,4-DBP) > 2,4-dichlorophenol (2,4-DCP), which implied that halogen substitution and the number of substituents in the halophenols could affect the generation of PFRs. The effects of transition-metal ions that resulted in the reduction of PFRs when adsorbed on the Mnt-HA complex were as follows: Fe3+ > Zn2+ > Cu2+ > Mn2+, and this is in agreement with their redox capacity. Analyzing the induced generation of reactive oxygen species (ROS) and electrons on POM, it is found that halophenols and transition metal ions also affected this process under irradiation. These findings indicate that the photoformation of PFRs on POM could be a source of PFRs in aqueous environments and requires further attention.


Assuntos
Bentonita , Material Particulado , Radicais Livres , Substâncias Húmicas , Espécies Reativas de Oxigênio
16.
Environ Sci Technol ; 54(20): 13175-13185, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-32985863

RESUMO

Extensive application of antibiotics leads to their ubiquitous occurrence in coastal aquatic environments. However, it remains largely unknown whether antibiotics can be bioaccumulated and biotransformed in major mariculture organisms such as sea cucumbers and toxicokinetic models for Echinodermata are lacking. In this study, laboratory exposure experiments on juvenile sea cucumber (Apostichopus japonicus) were performed for seven antibiotics (sulfadiazine, sulfamethoxazole, trimethoprim, enrofloxacin, ofloxacin, clarithromycin, and azithromycin). Field sea cucumber and surrounding seawater samples were also analyzed. Results show that the sea cucumbers tend to accumulate high concentrations of the antibiotics with kinetic bioconcentration factors (BCFs) up to 1719.7 L·kg-1 for ofloxacin. The BCFs determined in the laboratory agree well with those estimated from the field measurements. Seven biotransformation products (BTPs) of the antibiotics were identified, four of which were not reported previously in aquatic organisms. The BTPs were mainly found in the digestive tract, indicating its high capacity in the biotransformation. A multicompartmental toxicokinetic model based on the principles of passive diffusion was developed, which can successfully predict time-course concentrations of the antibiotics in different compartments of the juvenile sea cucumbers. The findings may offer a scientific basis for assessing health risks and guiding healthy mariculture of sea cucumbers.

17.
Water Res ; 185: 116239, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32739702

RESUMO

Synthetic musks (SMs) are used extensively in household and personal care products and have acted significant concerns due to their environmental impacts and potential health effects. Here, we present a passive sampling approach based on diffusive gradients in thin films (DGT) for in situ measurement of SMs in urban wastewaters. XAD-2 binding gel, which has a rapid binding rate and high elution efficiency, was used in DGT device for the accumulation of six polycyclic musks and three nitro musks. The diffusion coefficients (D and DNL) of the SMs through agarose gel without and with a nylon filter membrane were 3.37-4.49 and 1.48-4.41 ×10-6cm2 s-1. The filter membrane caused an ~3 h lag phase and slowed the diffusion rates of the SMs through the diffusive phase. Solution pH (4.30-8.92), ionic strength (0.0001-0.5 M) and dissolved organic matter (0-20 mg L-1) showed no obvious influence on uptake of the SMs in DGT. The measured average SM concentrations in the effluent of wastewater treatment plants ranged from 0.45-696 ng/L for DGT deployment, without obvious membrane biofouling, and they were comparable to the concentrations determined by grab sampling. These results confirmed that the present method is reliable and convenient for in situ measurement of semivolatile hydrophobic SMs in complicated waters and is an available tool to investigate the environmental behaviors of SMs in the environment.


Assuntos
Águas Residuárias , Poluentes Químicos da Água , Difusão , Monitoramento Ambiental , Concentração Osmolar , Águas Residuárias/análise , Poluentes Químicos da Água/análise
18.
Front Microbiol ; 11: 1715, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32849362

RESUMO

Honey bee viruses are one of the most important pathogens that have contributed to the decrease in honey bee colony health. To analyze the infection dynamics of honey bee viruses, quantification of viral gene expression by RT-qPCR is necessary. However, suitable reference genes have not been reported from viral and RNAi studies of honey bee. Here, we evaluated the expression of 11 common reference genes (ache2, rps18, ß-actin, tbp, tif, rpl32, gadph, ubc, α-tubulin, rpl14, and rpsa) from Apis mellifera (Am) and Apis cerana (Ac) under Israeli acute paralysis virus (IAPV), chronic bee paralysis virus (CBPV), and Chinese sacbrood virus (CSBV) infection as well as dsRNA-PGRP-SA treatment, and we confirmed their validation by evaluating the levels of the defensin 1 and prophenoloxidase (ppo) genes during viral infection. Our results showed that the expression of selected genes varied under different viral infections. ache2, rps18, ß-actin, tbp, and tif can be used to normalize expression levels in Apis mellifera under IAPV infection, while the combination of actin and tif is suitable for CBPV-infected experiments. The combination of rpl14, tif, rpsa, ubc, and ache2 as well as more reference genes is suitable for CSBV treatment in Apis cerana. Rpl14, tif, rps18, ubc, and α-tubulin were the most stable reference genes under dsRNA treatment in Apis mellifera. Furthermore, the geNorm and NormFinder algorithms showed that tif was the best suitable reference gene for these four treatments. This study screened and validated suitable reference genes for the quantification of viral levels in honey bee, as well as for RNAi experiments.

19.
Arch Gynecol Obstet ; 302(3): 569-578, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32638096

RESUMO

PURPOSE: This study aimed to explore the association between vaginal microbiota and infertility. METHODS: We searched a range of electronic databases for appropriate articles, including PubMed, Web of Science, Embase, Chinese National Knowledge Infrastructure (CNKI), and Wanfang, from inception to 8th September 2019. Identified articles were then screened using strict inclusion and exclusion criteria. By referring to Tamarelle's method, we divided vaginal microbiota into two categories: low-Lactobacillus vaginal microbiota (LL-VMB) and high-Lactobacillus vaginal microbiota (HL-VMB). Patients were defined as HL-VMB if they had a Nugent score of 0-3, a negative Amesel/Spiegel's test, or if the vaginal community status was dominated by either L. crispatus, L. iners, L. gasseri and L. jensenii via 16S rRNA sequencing. Otherwise, cases were regarded as LL-VMB. Statistical analyses were performed with STATA 13.0 statistical software. Effect estimates are presented as odds ratios (ORs) with 95% confidence intervals (CIs). RESULTS: Fifteen articles were included in our final analysis. The HL-VMB was negatively related to infertility; a fixed model showed that the pooled OR was 0.83 (95% CI 0.77-0.90). There was no significant publication bias, as determined by Begg's test (P = 0.488) and Egger's test (P = 0.652). Using a random effect model, the pooled OR for intermediate bacterial vaginitis (BV) and infertility was 1.39 (95% CI 1.10-1.76) and the pooled OR for positive BV was 1.72 (95% CI 1.10-2.69). Subgroup and sensitivity analyses further demonstrated that the associations identified were stable. However, the acquired evidence was not sufficient to make inferences with regards to the mechanisms underlying these relationships. CONCLUSION: This systematic review and meta-analysis identified a negative correlation between HL-VMB and female infertility. However, due to a variety of limitations, the evidence acquired does not allow us to identify the specific mechanisms underlying this association. Further high-quality studies are needed to verify the causal relationship and explore the molecular mechanisms involved.


Assuntos
Infertilidade Feminina/etiologia , Lactobacillus/genética , RNA Ribossômico 16S/genética , Vagina/microbiologia , Feminino , Humanos , Microbiota , Vaginose Bacteriana/microbiologia
20.
Nutr Metab (Lond) ; 17: 39, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32489394

RESUMO

Background: Accumulating evidence shows that circulating levels of trimethylamine N-oxide, which is generated from the metabolism of dietary choline, may predict cardiovascular disease among Caucasians. Acute coronary syndrome (ACS), one common presentation of cardiovascular disease, is a spectrum of signs and symptoms due to acute decreased blood flow in the coronary arteries. The relationship between the metabolites from choline pathway and ACS remains unclear. We aimed to assess the associations of circulating metabolites from the choline pathway with ACS among a Chinese population, who consume a different dietary pattern than their Western counterparts. Methods: We recruited 501 participants who were admitted to the Department of Cardiology, Zhongshan Hospital,Shanghai China between March 2017 and June 2018, including 254 ACS cases and 247 controls. Liquid chromatography-tandem mass spectrometry was used to measure circulating concentrations of metabolites in the choline pathway, including betaine, choline, trimethylamine, and trimethylamine N-oxide. A composite metabolite score using a weighted sum of these four metabolites, and the betaine/choline ratio were calculated. Multivariable logistic regressions were applied to estimate the association of metabolites with ACS, with adjustment of age, sex, body mass index, smoking index, history of diseases, and kidney function. Results: After adjusting for traditional risk factors, per 1-standard deviation (SD) increment in choline was positively associated with the odds of ACS [odds ratio (OR), 95% confidence interval (CI), 1.77(1.44-2.18)], and the other metabolites were not associated with ACS at a statistical significance level. Compared with participants in the lowest quartile of the metabolite score, those in the highest quartile had higher odds of ACS [OR (95% CI), 3.18(1.85-5.54), p < 0.001 for trend]. Per 1-SD increment in metabolite score was positively associated with higher odds of ACS [OR (95% CI), 1.80 (1.37-2.40)], and per 1-SD increment in the betaine/choline ratio was inversely associated with the odds of ACS [OR (95% CI), 0.49 (0.39-0.60)]. Conclusions: Among our Chinese participants, trimethylamine N-oxide was not associated with ACS, while a composite metabolite score of metabolites from the choline pathway was associated with increased odds of ACS. The choline pathway metabolites may be related to the pathophysiology of ACS among Chinese.

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