Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 372
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-36728539

RESUMO

OBJECTIVE: To develop and validate a clinicoradiomic nomogram based on sagittal T2WI images to predict placental accreta spectrum (PAS). METHODS: Between October 2016 and April 2022, women suspected PAS by ultrasound (US) were enrolled. With the excluded criterial, 132 women were retrospectively included in the study. The variance threshold, SelectKBest and the least absolute shrinkage and selection operator (LASSO) were applied to select radiomic feature, which was further used to calculate the Rad-score. Multivariable logistic regression was used to screen clinical factor. RESULTS: Based on 13 radiomic features, 5 radiomic models were constructed. A clinical factor of ITHB (intraplacental T2-hypointense bands )was obtained by multivariate logistic regression. The AUC value of SGD(Stochastic Gradient Descent) radiomic model is 0.82 in the train cohort and 0.78 in the test cohort. After adding clinical factor to the SGD radiomic model, the AUC value of clinicoradiomic model was significantly increased from 0.82 and 0.78 to 0.84 in both the train and test cohort. The nomogram of clinicoradiomic model was constructed, which had good performance verified by calibration and decision curve. CONCLUSION: The presented nomogram could be useful for predicting placental accreta spectrum (PAS).

2.
Int J Mol Sci ; 24(2)2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36675029

RESUMO

Osteoprotegerin (OPG) is a new member of the tumor necrosis factor (TNF) receptor superfamily, which can inhibit the differentiation and activity of osteoclasts by binding to nuclear factor kappa B receptor activator (RANK) competitively with nuclear factor kappa B receptor activator ligand (RANKL). The previous experiments found that OPG can induce apoptosis of mature osteoclasts in vitro, which can inhibit the activity of mature osteoclasts, thereby exerting its role in protecting bone tissue. In addition, pyroptosis is a new type of cell death that is different from apoptosis. It is unclear whether OPG can induce mature osteoclast pyroptosis and thereby play its role in protecting bone tissue. In this study, the results showed that compared with the control group, the survival rate of osteoclasts in the OPG group was significantly reduced, and the contents of IL-1ß, IL-18, and LDH in the supernatant both increased. Many osteoclast plasma membranes were observed to rupture in bright fields, and OPG induced loss of their morphology. Flow cytometry was used to analyze the pyroptosis rate; OPG significantly increased the osteoclast pyroptosis rate. To further reveal the mechanism of OPG-induced osteoclast pyroptosis, we examined the expression level of pyroptosis-related genes and proteins, and the results found that OPG increased the expression of NLRP3, ASC, caspase-1, and GSDMD-N compared with the control group. In summary, OPG can induce osteoclast pyroptosis, and its mechanism is related to the expression levels of ASC, NLRP3, caspase 1 and GSDMD, which were included in the classical pathway of pyroptosis.


Assuntos
Osteoclastos , Osteoprotegerina , Osteoclastos/metabolismo , Osteoprotegerina/genética , Osteoprotegerina/metabolismo , Glicoproteínas/metabolismo , Glicoproteínas de Membrana/genética , NF-kappa B/metabolismo , Piroptose , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Osteoblastos/metabolismo , Receptores Citoplasmáticos e Nucleares/metabolismo , Ligante RANK/metabolismo
3.
RSC Adv ; 13(2): 1312-1319, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36686910

RESUMO

The development of sorbents for flue gas desulfurization in a dry mode is essential to control emission of sulfur dioxide. Based on the novel concept of "treating waste with waste", a low-cost and highly activated calcium-based sorbent (ACS) was prepared using coal fly ash, CaO and waste gypsum as the raw materials via the one-step incipient wetness impregnation method. Based on characterization using scanning electron microscopy and nitrogen adsorption-desorption, the ACS possessed a fibrous and netted structure with high porosity, which improved SO2 adsorption greatly. The SO2 adsorption capacity of ACS with coal fly ash/CaO/CaSO4 = 1/2/1 was high, up to 44.26 mg g-1, with 100% removal efficiency at 150 °C. In the absence of O2, SO2 was rapidly adsorbed on the sorbent to form CaSO3 according to in situ DRIFTS analysis, while when O2 was present in the flue gas, SO2/SO3 2- tended to be oxidized into SO4 2- species. Moreover, the presence of NO can further enhance the SO2 adsorption capacity of the ACS due to the formation of adsorbed NO2 or nitrate species with strong oxidizing properties. Therefore, the ACS can be considered as a sustainable sorbent with the advantage of employing fly ash for the removal of sulfur dioxide.

4.
Sci Rep ; 13(1): 1196, 2023 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-36681714

RESUMO

Calmodulin-like proteins (CML) are important calcium signal transduction proteins in plants. CML genes have been analyzed in several plants. However, little information on CML in Phaseolus vulgare is available. In this study, we identified 111 PvCMLs distributed on eleven chromosomes. Phylogenetic analysis classified them into seven subfamilies. Cis-acting element prediction showed that PvCML contained elements related to growth and development, response to abiotic stress and hormones. Moreover, the majority of PvCMLs showed different expression patterns in most of the nine tissues and developmental stages which indicated the role of PvCML in the growth and development of common bean. Additionally, the common bean was treated with melatonin by seed soaking, and root transcriptome at the 5th day and qRT-PCR of different tissue at several stages were performed to reveal the response of PvCML to the hormone. Interestingly, 9 PvCML genes of subfamily VI were detected responsive to exogenous melatonin, and the expression dynamics of nine melatonin response PvCML genes after seed soaking with melatonin were revealed. Finally, the protein interaction network analysis of nine melatonin responsive PvCMLs was constructed. The systematic analysis of the PvCML gene family provides theoretical support for the further elucidation of their functions, and melatonin response molecular mechanism of the CML family in P. vulgaris.


Assuntos
Melatonina , Phaseolus , Phaseolus/metabolismo , Melatonina/farmacologia , Melatonina/metabolismo , Filogenia , Transcriptoma , Estresse Fisiológico/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Regulação da Expressão Gênica de Plantas
5.
Int J Biol Sci ; 19(2): 691-704, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36632452

RESUMO

Cervical cancer (CC) is one of the most common gynecological malignancies with poor prognosis for advanced CC patients. LRRC8A is a volume-regulated anion channel protein involved in cellular homeostasis, but its role in CC remains largely unknown. In this study, we found that LRRC8A is elevated in CC and associated with poor prognosis. LRRC8A maintains cell survivals under the hypotonic condition, and promotes tumorigenesis through apoptosis suppression in vitro and in vivo. Notably, LRRC8A is upregulated by NSUN2-mediated m5C modification. m5C modified-LRRC8A mRNA is bound by the RNA binding protein YBX1 followed by the increased RNA stability. Moreover, loss of NSUN2 suppresses the proliferation and metastasis of CC cells, and NSUN2 expression is positively correlated with LRRC8A expression in CC. Altogether, our study demonstrates that the NSUN2-m5C-LRRC8A axis is crucial and would be a potential therapeutic target for CC.


Assuntos
Proteínas de Membrana , Neoplasias do Colo do Útero , Feminino , Humanos , Regulação para Cima/genética , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Neoplasias do Colo do Útero/genética , Carcinogênese/genética , Transformação Celular Neoplásica , Apoptose/genética , Proliferação de Células/genética
6.
J Ethnopharmacol ; 305: 116119, 2023 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-36596398

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Rheumatoid arthritis (RA) is a common systemic autoimmune disease with high morbidity and disability rate. Currently, there is no effective allopathic treatment for RA, and most of the drugs provoke many adverse effects. Simiao Yong'an decoction (SMYAD) is a traditional Chinese prescription for the treatment of sore and gangrene caused by hot poison. With the development of pharmacology and clinical research, SMYAD has remarkable anti-inflammatory properties and has been used for RA treatments for years. AIM OF THE STUDY: This study aimed to investigate the anti-arthritic effect of SMYAD and further explore the immunopharmacological mechanisms. MATERIALS AND METHODS: Arthritis was induced in DBA/1 mice by two-time immunizations. Collagen-induced rheumatoid arthritis (CIA) mice were divided into 4 groups: control, model, methotrexate (MTX), and SMYAD group (n = 6). The administration groups were given MTX (0.5 mg/kg/3 d) and SMYAD (4.5 g/kg/d) by gavage from day 14. The arthritis index (AI) score was evaluated every 3 days after the second immunization. Hematoxylin and eosin (H&E) staining, Safranin-O fast green staining, Trap staining, and Micro-CT were used to measure the histopathology injuries and bone destruction of joints. Granulocyte changes in the spleen, bone marrow, and period blood were analyzed by flow cytometry. The expression of inflammatory cytokines and chemokines in joints were detected by qRT-PCR. SMYAD-containing serum was obtained from SD rats gavaged with SMYAD. Neutrophils were isolated from peripheral blood and bone marrow for the in vitro experiments of transwell cell assay, apoptosis assay, reactive oxygen species (ROS) generation and neutrophil extracellular traps (NETs) formation. RESULTS: SMYAD significantly relieved arthritis severity in CIA mice. The AI score was significantly decreased in the SMYAD group compared with the model group. Additionally, SMYAD alleviated inflammatory infiltration, cartilage damage, osteoclast formation, and bone damage in the ankle joints. In the flow cytometry assay, SMYAD significantly reduced granulocytes number in the spleen and bone marrow, while increased in peripheral blood. Furthermore, compared with the CIA group, SMYAD suppressed the mRNA levels of inflammatory factors including TNF-α, IL-1ß, IL-6 and chemokines CXCL1, CXCL2, and IL-8 in the inflamed joints. In the in vitro studies, 20% SMYAD-containing serum effectively inhibited the migration of neutrophils, promoted neutrophils apoptosis, reduced ROS production and NETs formation. CONCLUSION: Collectively, our results demonstrated that SMYAD effectively restrained arthritis in CIA mice by modulating neutrophil activities.


Assuntos
Artrite Experimental , Artrite Reumatoide , Camundongos , Ratos , Animais , Artrite Experimental/patologia , Neutrófilos/metabolismo , Espécies Reativas de Oxigênio , Ratos Sprague-Dawley , Camundongos Endogâmicos DBA , Artrite Reumatoide/tratamento farmacológico , Citocinas/metabolismo , Metotrexato
7.
Acta Cir Bras ; 37(12): e371202, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36651427

RESUMO

PURPOSE: To investigate the mechanism of polysaccharides from aloe vera (PAV), a main active ingredient of Aloe vera, treatment in pulpitis rats. METHODS: Pulpitis were modeled by drilling the occlusal central fossa with Sprague Dawley rats. Next, the rats were treated with 20, 40, and 80 mg/kg PAV for three weeks, respectively. Computed tomography scanning assay, hematoxylin and eosin staining, and tartrate-resistant acid phosphatase staining were used to detect the pathology change. Then, levels of tumor necrosis factor-α, interleukin-1ß, prostaglandin E2, and ciclooxigenase 2 were detected by enzyme-linked immunosorbent assay. The expressions of bone morphogenetic protein 2 human (BMP-2), osteocalcin, osterix, and runt-related transcription factor 2 (Runx2) were quantified by quantitative real-time polymerase chain reaction and Western blotting (WB). Finally, Wnt3a expression, p-GSK3ß/GSK3ß and p-ß-catenin/ß-catenin ratio were analyzed by WB. RESULTS: PAV up regulated the bone mineral density, and reduced the breakage of the crown and cervical structures, and the necrosis of the crown and root pulp of pulpitis rats. In addition, results indicated that PAV could inhibit osteoblast formation. While osteoblasts' number was decreased, proteins of BMP-2, osteocalcin, osterix, and Runx2 were up-regulated by PAV. Furthermore, PAV increased the Wnt3a expression and the p-ß-catenin/ß-catenin ratio, and decreased p-GSK3ß/GSK3ß ratio. Interestingly, these effects were all in dose dependence. CONCLUSIONS: PAV could inhibit pulp inflammation and promote osteoblasts differentiation via suppressing the activation of the Wnt/ß-catenin signaling, enhancing the dental bone density.


Assuntos
Aloe , Polissacarídeos , Pulpite , Via de Sinalização Wnt , Animais , Humanos , Ratos , Aloe/química , beta Catenina/metabolismo , Diferenciação Celular , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Subunidade alfa 1 de Fator de Ligação ao Core/farmacologia , Glicogênio Sintase Quinase 3 beta/metabolismo , Osteoblastos , Osteocalcina/metabolismo , Osteogênese , Polissacarídeos/farmacologia , Pulpite/metabolismo , Ratos Sprague-Dawley
8.
Sci Total Environ ; 857(Pt 1): 159339, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36228798

RESUMO

To avoid the spread of COVID-19, China implemented strict prevention and control measures, resulting in dramatic variations in urban and regional air quality. With the complex effect from long-term emission mitigation and meteorology variation, an accurate evaluation of the net effect from lockdown on air quality changes has not been fully quantified. Here, we combined machine learning algorithm and Theil-Sen regression technique to eliminate meteorological and long-term trends effects on air pollutant concentrations and precisely detect concentrations changes those ascribed to lockdown measures in North China. Our results showed that, compared to the same period in 2015-2019, the adverse meteorology during the lockdown period (January 25th to March 15th) in early 2020 increased PM2.5 concentration in North China by 9.8 %, while the reduction of anthropogenic emissions led to a 32.2 % drop. Stagnant meteorological conditions have a more significant impact on the ground-level air quality in the Beijing-Tianjin-Hebei Region than that in Shanxi and Shandong provinces. After further striping out the effect of long-term emission reduction trend, the lockdown-derived NO2, PM2.5, and O3 shown variety change trend, and at -30.8 %, -27.6 %, and +10.0 %, respectively. Air pollutant changes during the lockdown could be overestimated up to 2-fold without accounting for the influences of meteorology and long-term trends. Further, with pollution reduction during the lockdown period, it would avoid 15,807 premature deaths in 40 cities. If with no deteriorate meteorological condition, the total avoided premature should increase by 1146.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , COVID-19 , Humanos , COVID-19/epidemiologia , Material Particulado/análise , Saúde Pública , Monitoramento Ambiental/métodos , Controle de Doenças Transmissíveis , Poluição do Ar/análise , Poluentes Atmosféricos/análise , Cidades , China/epidemiologia , Aprendizado de Máquina
9.
J Control Release ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36464063

RESUMO

Tumor-derived exosomes (TDEs) are the particular communicator and messenger between tumor cells and other cells containing cancer-associated genetic materials and proteins. And TDEs who are also one of the important component consisting of the tumor microenvironment (TME) can reshape and interact with TME to promote tumor development and metastasis. Moreover, due to their long-distance transmission by body fluids, TDEs can facilitate the formation of pre-metastatic niche to support tumor colonization. We discuss that the main characteristics and mechanism of TDE-mediated tumor metastasis by reshaping TME and pre-metastatic niche as well as the potential of TDEs for diagnosing tumor and predicting future metastatic development.

10.
Front Plant Sci ; 13: 979540, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36570946

RESUMO

Wheat is one of the most important food crops in the world and is considered one of the top targets in crop biotechnology. With the high-quality reference genomes of wheat and its relative species and the recent burst of genomic resources in Triticeae, demands to perform gene functional studies in wheat and genetic improvement have been rapidly increasing, requiring that production of transgenic wheat should become a routine technique. While established for more than 20 years, the particle bombardment-mediated wheat transformation has not become routine yet, with only a handful of labs being proficient in this technique. This could be due to, at least partly, the low transformation efficiency and the technical difficulties. Here, we describe the current version of this method through adaptation and optimization. We report the detailed protocol of producing transgenic wheat by the particle gun, including several critical steps, from the selection of appropriate explants (i.e., immature scutella), the preparation of DNA-coated gold particles, and several established strategies of tissue culture. More importantly, with over 20 years of experience in wheat transformation in our lab, we share the many technical details and recommendations and emphasize that the particle bombardment-mediated approach has fewer limitations in genotype dependency and vector construction when compared with the Agrobacterium-mediated methods. The particle bombardment-mediated method has been successful for over 30 wheat genotypes, from the tetraploid durum wheat to the hexaploid common wheat, from modern elite varieties to landraces. In conclusion, the particle bombardment-mediated wheat transformation has demonstrated its potential and wide applications, and the full set of protocol, experience, and successful reports in many wheat genotypes described here will further its impacts, making it a routine and robust technique in crop research labs worldwide.

11.
J Mol Histol ; 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36417034

RESUMO

Circular RNAs (circRNAs) have been shown to be associated with cardiac fibrosis. Atrial fibrosis is an important pathophysiological event in the progression of atrial fibrillation (AF). Although a novel circRNA calmodulin binding transcription activator 1 (circCAMTA1) has been reported to be related with the development of AF, the detailed molecular mechanisms remain largely unknown. In this study, we found that circCAMTA1 was upregulated in atrial muscle tissues of AF patients and angiotensin-II (Ang-II)-treated human atrial fibroblasts (HAFs). Moreover, circCAMTA1 expression was positively correlated with the expression of collagen (I and III) and α-SMA in atrial muscle tissues of AF patients. In vitro experiments, knockdown of circCAMTA1 significantly suppressed Ang-II-induced HAFs proliferation and reduced the expression of atrial fibrosis-associated genes, but overexpression of circCAMTA1 exhibited opposite results. In vivo experiments, circCAMTA1 knockdown ameliorated Ang-II-induced atrial fibrosis by reducing AF incidence, AF duration, and collagen synthesis. Functionally, circCAMTA1 facilitated Ang-II-induced atrial fibrosis in vitro and in vivo via downregulating the inhibitory effect of miR-214-3p on transforming growth factor ß receptor 1 (TGFBR1) expression. In conclusions, circCAMTA1 knockdown alleviated atrial fibrosis through downregulating TGFBR1 expression intermediated by miR-214-3p in AF, suggesting circCAMTA1/miR-214-3p/TGFBR1 axis may be a novel therapeutic target for AF treatment in clinic.

12.
Sci Total Environ ; 859(Pt 1): 160172, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36395856

RESUMO

Unexpected outbreak of the 2019 novel coronavirus (COVID-19) has profoundly altered the way of human life and production activity, which posed visible impacts on PM2.5 and its chemical species. The abruptly emergency reduction in human activities provided an opportunity to explore the synergetic impacts of multi-factors on shaping PM2.5 pollution. Here, we conducted two comprehensive observation measurements of PM2.5 and its chemical species from 1 January to 16 February in Beijing 2020 and the same lunar date in 2021, to investigate temporal variations and reveal the driving factors of haze before and after Chinese New Year (CNY). Results show that mean PM2.5 concentrations during the whole observation were 63.83 and 66.86 µg/m3 in 2020 and 2021, respectively. Higher secondary inorganic species were observed after CNY, and K+, Cl- showed three prominent peaks which associated closely with fireworks burnings from suburb Beijing and surroundings, verifying that they could be used as two representative tracers of fireworks. Further, we explored the impacts of meteorological conditions, regional transportation as well as chemical reactions on PM2.5. We found that unfavorable meteorological conditions accounted for 11.0 % and 16.9 % of PM2.5 during CNY holidays in 2020 and 2021, respectively. Regional transport from southwest and southeast (south) played an important role on PM2.5 during the two observation periods. Higher ratio of NO3-/SO42- were observed under high OX and low RH conditions, suggesting the major pathway of NO3- and SO42- formation could be photochemical process and aqueous-phase reaction. Additionally, nocturnal chemistry facilitated the formation of secondary components of both inorganic and organic. This study promotes understandings of PM2.5 pollution in winter under the influence of COVID-19 pandemic and provides a well reference for haze and PM2.5 control in future.

13.
Int J Mol Sci ; 23(21)2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36362277

RESUMO

Environmental and occupational exposure to cadmium has been shown to induce kidney damage, liver injury, neurodegenerative disease, and osteoporosis. However, the mechanism by which cadmium induces autophagy in these diseases remains unclear. Studies have shown that cadmium is an effective inducer of oxidative stress, DNA damage, ER stress, and autophagy, which are thought to be adaptive stress responses that allow cells exposed to cadmium to survive in an adverse environment. However, excessive stress will cause tissue damage by inducing apoptosis, pyroptosis, and ferroptosis. Evidently, oxidative stress-induced autophagy plays different roles in low- or high-dose cadmium exposure-induced cell damage, either causing apoptosis, pyroptosis, and ferroptosis or inducing cell survival. Meanwhile, different cell types have different sensitivities to cadmium, which ultimately determines the fate of the cell. In this review, we provided a detailed survey of the current literature on autophagy in cadmium-induced tissue damage. A better understanding of the complex regulation of cell death by autophagy might contribute to the development of novel preventive and therapeutic strategies to treat acute and chronic cadmium toxicity.


Assuntos
Doenças Neurodegenerativas , Síndromes Neurotóxicas , Humanos , Cádmio/metabolismo , Doenças Neurodegenerativas/metabolismo , Autofagia , Estresse Oxidativo , Rim/metabolismo , Apoptose , Fígado/metabolismo , Síndromes Neurotóxicas/metabolismo
14.
J Econ Entomol ; 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-36240118

RESUMO

Orius sauteri (Poppius) is an important predator of many economically important insect pests. The mass rearing of O. sauteri is difficult, limiting its application in pest control. Here we assessed the nutritional quality of eggs of Sitotroga cerealella (Olivier), Agrotis ypsilon (Rottemberg), or Spodoptera litura (Fabricius), and their potential for rearing O. sauteri in the laboratory for two generations. Of species tested, S. cerealella eggs resulted in the highest survival and reproduction of O. sauteri compared to the other two lepidopteran species. Eggs of A. ypsilon were a suitable diet for the nymphal stage, which developed faster on A. ysilon eggs than those of S. cerealella eggs. Conversely, eggs of S. litura were not a suitable diet for O. sauteri, and they disrupted the development and reproduction of O. sauteri. Sitotroga cerealella eggs showed advantages in all the nutritional components evaluated. Orius sauteri fed S. litura eggs contained significantly lower protein levels than those fed on the other eggs tested. Spodoptera litura eggs significantly enhanced the CAT activity in O. sauteri, which suggests that some components from S. litura eggs harmed the development and reproduction of O. sauteri. Based on these results, we suggest using a combined diet for mass rearing of the pirate bug, feeding the nymphs and adults with A. ypsilon eggs and S. cerealella eggs, respectively. This study contributes to the discovery of artificial diets for mass rearing O. sauteri and other Orius species in the future.

15.
Front Pharmacol ; 13: 1010640, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36249763

RESUMO

Yi Shen Juan Bi Pill (YSJB) is a traditional Chinese medicine (TCM) formulation that has a therapeutic effect upon rheumatoid arthritis (RA), but how YSJB affects bone destruction in arthritis under postmenopausal conditions is not known. We evaluated the therapeutic role of YSJB in bone destruction in postmenopausal arthritis, We used collagen-induced arthritis (CIA) rats who had been ovariectomized (OVX) as models and explored the possible mechanism from the synovium and bone marrow (BM). Arthritis was generated after ovariectomy or sham surgery for 12 weeks. After 14 days of primary immunization, rats were administered YSJB or estradiol valerate (EV) for 28 days. YSJB could prevent bone destruction in the inflamed joints of rats in the OVX + CIA group. CIA promoted osteoclast differentiation significantly in the synovial membrane according to tartrate resistant acid phosphatase (TRACP) staining, and OVX tended to aggravate the inflammatory reaction of CIA rats according to hematoxylin-and-eosin staining. Immunohistochemistry revealed that the synovium did not have significant changes in erythropoietin-producing hepatocellular interactor (ephrin)B2 or erythropoietin-producing hepatocellular (eph) B4 expression after YSJB treatment, but YSJB treatment reduced nuclear factor of activated T cells (NFATc)1 expression. The BM of rats in the OVX + CIA exhibited remarkable increases in the number of osteoclasts and NFATc1 expression, as well as significantly reduced expression of ephrinB2 and ephB4 compared with the CIA group and sham group. YSJB treatment reduced NFATc1 expression significantly but also increased ephrinB2 expression in the BM markedly. These data suggest that YSJB exhibit a bone-protective effect, it may be a promising therapeutic strategy for alleviating bone destruction in arthritis under postmenopausal conditions, and one of the mechanisms is associated with the modulation of ephrinB2 signaling.

16.
Environ Sci Ecotechnol ; 12: 100203, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36157339

RESUMO

China is striving to build a "Beautiful China" characterized by clean air. The country has committed to further reducing its national mean fine particle (PM2.5) concentration by 10% from 2020 to 2025, following the substantial improvements in its air quality during the past decade. Meanwhile, the "Healthy China" mission has pledged to increase the national mean life expectancy by one year during the same period. Yet, to what extent will the "Beautiful China" mission contribute to the "Healthy China" vision by reducing the levels of the detrimental PM2.5 is still unclear. Here, by coupling the life table approach and an epidemiological concentration-response model, this study quantifies the potential benefits of achieving China's 2025 air quality target on the national life expectancy. The analysis reveals that the Chinese citizen could expect to extend the average life expectancy by 42.5 days by 2025 due to improved air quality. In addition, if the Chinese government outperforms the planned air quality target, as it usually does, the gains would increase to 65.4 days, ∼18% of the "Healthy China" life expectancy increment task. Further reductions in PM2.5 concentration would lead to accelerated gains in life expectancy both nationally and at the city level, providing strong incentives for the authorities to keep improving air quality. This study reveals the notable benefits on individual life that could be expected from air quality improvement in China and suggests that longer life expectancy is achievable by implementing a health-prioritized air quality management mechanism.

17.
Front Cardiovasc Med ; 9: 872833, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36158823

RESUMO

Objective: To identify the pregnancy outcomes and risk factors of critically ill pulmonary hypertension (PH) patients with intensive care unit (ICU) admission. Methods: The multicenter, retrospective cohort study was performed on 60,306 parturients from January 2013 to December 2018 in China. Diagnosis of PH was based on the estimation of systolic pulmonary arterial pressure (sPAP) via echocardiography. Patients were stratified by sPAP into three groups, mild (30-50 mmHg), moderate (51-70 mmHg), and severe (>70 mmHg). The primary outcome was major adverse cardiovascular events (MACE), defined as a composite of in-hospital death, heart failure, and sustained arrhythmias requiring treatment. The secondary outcome was fetal adverse clinical events (FACE), a composite of fetal/neonatal death, prematurity, small birth weight, and fetal distress. Results: A total of 181 pregnant patients were enrolled, including 101 patients with mild PH, 31 with moderate PH, and 49 with severe PH. The maternal median age was 32 (27, 35) years and 37% were nulliparous. The MACE occurred in 59 (59/181, 32.6%) women, including in-hospital death in 13 (13/181, 7.2%), heart failure in 53 (53/181, 29.3%), and sustained arrhythmias in 7 (7/181, 3.9%). The incidence of FACE was as high as 66.3% (120/181). Compared with mild and moderate PH patients, patients with severe PH had a significantly higher mortality rate (22.4 vs. 1.51%, P < 0.001) and MACE incidence (51.0 vs. 25.8%, P = 0.001). Although the incidence of FACE in severe PH was slightly higher than that in mild to moderate PH, there was no significant difference (69.4 vs. 65.1%, P = 0.724). PH complicated with left heart disease (OR = 4.365, CI: 1.306-14.591), elevated N-terminal pro-B-type natriuretic peptide (NT-proBNP) level (OR = 1.051, CI:1.015-1.088), and sPAP level estimated by echocardiography (OR = 1.021; CI: 1.003-1.040) were independently associated with MACE in multivariable regression (P < 0.05). Increased risk of FACE was noted for PH patients combined with eclampsia/preeclampsia (OR = 6.713; CI: 1.806-24.959). Conclusion: The incidence of MACE and FACE remained high in critically ill pregnant patients with PH, particularly moderate and severe PH in China. Further studies are warranted to identify subsets of women with PH at lower pregnant risks and seek more effective therapy to improve pregnancy outcomes.

18.
Biomedicines ; 10(9)2022 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-36140414

RESUMO

In recent years, although resin composite has played an important role in the restoration of tooth defects, it still has several disadvantages, including being biodegraded by saliva, bacteria and other enzymes in the oral cavity, which may result in repair failure. This factor is not conducive to the long-term survival of the prosthesis in the mouth. In this article, we review the causes, influencing factors and prevention methods of resin biodegradation. Biodegradation is mainly caused by esterase in saliva and bacteria, which breaks the ester bond in resin and causes the release of monomers. The mechanical properties of the prosthesis can then be affected. Meanwhile, cathepsin and MMPs are activated on the bonding surface, which may decompose the dentin collagen. In addition, neutrophils and residual water on the bonding surface can also aggravate biodegradation. Currently, the primary methods to prevent biodegradation involve adding antibacterial agents to resin, inhibiting the activity of MMPs and enhancing the crosslinking of collagen fibers. All of the above indicates that in the preparation and adhesion of resin materials, attention should be paid to the influence of biodegradation to improve the prosthesis's service life in the complex environment of the oral cavity.

19.
Toxins (Basel) ; 14(8)2022 08 22.
Artigo em Inglês | MEDLINE | ID: mdl-36006235

RESUMO

Two recently revised Azemiops snakes with apparent differences in their external appearances and skeletal morphologies but unclear genetic boundaries have been proposed. Some researchers have refrained from using the newly proposed taxonomy because these two "species" might be two clades corresponding to different geographical populations of Azemiops feae. To improve the understanding of the kinship of these two Burmese viper groups, more of their characteristics should be explored in depth. We performed a comparative analysis of the proteomic profiles and biochemical activities of snake venoms from these two groups (Sichuan A. feae and Zhejiang A. feae) and evaluated the immunorecognition capacity of commercial antivenoms toward them. Eight protein families were identified in venoms from these two groups, while phospholipase B was only detected in venom from Sichuan A. feae. These protein families displayed varying degrees of differences in relative abundance between venoms, and phospholipase A2 (Sichuan A. feae: 57.15%; Zhejiang A. feae: 65.94%) was the predominated component. Gloydius brevicaudus antivenom exhibited the strongest capacity to immunologically recognize these two venoms, but this was mainly limited to components with high molecular masses, some of which differed between venoms. Additionally, Zhejiang A. feae venom was more toxic than Sichuan A. feae venom, and the venoms expressed remarkable differences in enzymatic activities, probably resulting from the variation in the relative abundance of specific protein families. Our findings unveil differences between the two Burmese viper groups in terms of proteomic profiles, immunoreactivity, and the biochemical functions of their venoms. This information will facilitate the management of snakebites caused by these snakes.


Assuntos
Mordeduras de Serpentes , Viperidae , Animais , Antivenenos/metabolismo , Antivenenos/farmacologia , Proteínas/metabolismo , Proteômica/métodos , Venenos de Serpentes/química , Venenos de Víboras/química , Viperidae/metabolismo
20.
Poult Sci ; 101(10): 102047, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35973349

RESUMO

CD127, also named interleukin-7 receptor (IL-7R), is expressed on various cell types including naive and memory T cells, and plays a critical role in the differentiation and activation of T lymphocytes. The availability of poultry-specific immune reagents to identify and measure chicken CD127 response will enhance fundamental and applied research in poultry immunology. Mouse monoclonal antibodies (MAbs) against chicken CD127 (chCD127) were developed and characterized. More specifically, a 678 bp ectodomain of chCD127 gene was cloned in the pET28a (+) vector and expressed in BL21-AI E. coli competent cells. The recombinant chCD127 protein with a size of 30 KDa which was also recognized by a mouse anti-human CD127 MAb (Clone G-11) was used to immunize mice, and 6 new mouse MAbs which specifically detected chicken CD127 were developed and characterized. Availability of these new sets of chCD127-specific MAbs will facilitate the immunological studies on CD127 in poultry, especially in understanding effector and memory T immune cell responses in normal and diseased states.


Assuntos
Anticorpos Monoclonais , Galinhas , Subunidade alfa de Receptor de Interleucina-7/imunologia , Animais , Galinhas/genética , Escherichia coli , Interleucina-7 , Camundongos , Proteínas Recombinantes
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...