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1.
Med Sci Monit ; 26: e921822, 2020 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-32222722

RESUMO

BACKGROUND Acute kidney injury (AKI) is one of the most important causes of death in sepsis patients. Here, we first measured the level of DANCR (differentiation antagonizing nonprotein coding RNA) expression in AKI, and the potential mechanism was further elucidated. MATERIAL AND METHODS We used qRT-PCR to examine the level of DANCR in patient blood serum samples and in HK-2 cells. In addition, DANCR overexpression was established using lentiviral transfection in HK-2 cells. Subsequently, Cell Counting Kit-8 (CCK-8) assay and flow cytometry were applied to evaluate the role of DANCR in HK-2 cells treated with lipopolysaccharide (LPS). Enzyme linked immunosorbent assay (ELISA), western blot and recovery experiments were performed to elucidate the further mechanism. RESULTS We found that DANCR was decreased in the serum of AKI patients and HK-2 cells treated with LPS. Additionally, DANCR promoted cell viability and suppressed cell apoptosis and cytokine production in LPS-treated HK-2 cells. Bioinformatics analysis showed that DANCR served as a sponge for miR-214. Furthermore, DANCR inhibited the expression of Krüppel-like factor 6 (KLF6). CONCLUSIONS Our study suggests that AKI development could be alleviated by sponging miR-214 and regulating KLF6 expression, which provides a novel potential mechanism involved in the diagnosis and treatment of sepsis-induced AKI patients.

2.
Int J Mol Sci ; 20(19)2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31575049

RESUMO

A major fraction (MPT-W), eluted by deionized water, was extracted from mycelium polysaccharides of Termitomyces albuminosus (MPT), and its antioxidant, anti-fibrosis, and anti-inflammatory activities in CCl4-induced chronic liver injury mice, as well as preliminary characterizations, were evaluated. The results showed that MPT-W was a polysaccharide of α- and ß-configurations containing xylose (Xyl), fucose (Fuc), mannose (Man), galactose (Gal), and glucose (Glc) with a molar ratio of 0.29:8.67:37.89:35.98:16.60 by gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared (FT-IR) spectroscopy. Its molecular weight (Mw), obtained by high-performance gel permeation chromatography (HPGPC), was 1.30 × 105 Da. The antioxidant assays in vitro showed that MPT-W displayed scavenging free-radical abilities. Based on the data of in vivo experiments, MPT-W could inhibit TGFß1/Smad3 and NF-κB pathways; decrease the level and activity of cytochrome P4502E1 (CYP2E1), malonaldehyde (MDA) and serum enzyme; activate the HO-1/Nrf2 pathway; and increase antioxidant enzymes to protect the liver in CCl4-induced chronic liver injury mice. Therefore, MPT-W could be a potentially natural and functional resource contributing to antioxidant, hepatoprotective, and anti-inflammatory effects with potential health benefits.


Assuntos
Extratos Celulares/farmacologia , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Polissacarídeos Fúngicos/farmacologia , Micélio/química , Substâncias Protetoras/farmacologia , Transdução de Sinais/efeitos dos fármacos , Termitomyces/química , Animais , Extratos Celulares/química , Extratos Celulares/isolamento & purificação , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Doença Hepática Induzida por Substâncias e Drogas/patologia , Modelos Animais de Doenças , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/isolamento & purificação , Camundongos , NF-kappa B/metabolismo , Substâncias Protetoras/química , Proteína Smad3 , Análise Espectral , Fator de Crescimento Transformador beta1/metabolismo
3.
Oxid Med Cell Longev ; 2019: 8915272, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31583046

RESUMO

The acid-depolymerised exopolysaccharides (ADES) of Termitomyces albuminosus were obtained, and the major fraction of ADES1 was isolated and purified by DEAE-52 cellulose anion-exchange column chromatography. Physicochemical characterizations showed that ADES1 was an α- and a ß-configuration with the molecular weight of 2.43 kDa, containing (1→3, 4)-linked-Glcp, (1→4)-linked-D-Glcp, (1→3)-linked-D-Xylp, (1→4)-linked-D-Manp, T-Glcp, (1→6)-linked-D-Galp, and (1→4)-linked-L-Arap. The in vivo assays showed that ADES1 could reduce lipid levels in the serum and liver, decrease serum enzyme activities, and improve antioxidant enzyme activities and p-AMPKα expressions in hyperlipidemic mice, which were also confirmed by histopathological observations. These data indicated that ADES1 might be considered as a novel substance to treat and prevent hyperlipidemia and as a hepatoprotective agent.

4.
Molecules ; 24(15)2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31344969

RESUMO

The present work mainly describes the preparation of acetylated mycelia polysaccharides (AMPS) from Pleurotus djamor and investigates the antioxidant and anti-aging effects in d-galactose-induced aging mice. The optimized procedure indicates the acetyl substitution degree of AMPS is 0.54 ± 0.04 under the conditions of a reaction time of 56 h, a reaction temperature of 37 °C, and 4 mL of added acetic anhydride. The in vitro analysis and in vivo animal experiments indicate that the AMPS could alleviate the aging properties by scavenging the radicals, elevating the enzyme activities, and reducing the lipid contents. As for serum levels, the AMPS can improve the serum biochemical indices and enhance immunological activity. The histopathological observations indicate that the injuries to the liver, kidney, and brain can be remitted by AMPS intervention. The characterization showed that AMPS was one kind of ß-pyranose with the weight-average molecular weights of 3.61 × 105 Da and the major monosaccharides of mannose and glucose. The results suggest that AMPS can be used as a dietary supplement and functional food for the prevention of aging and age-related diseases.


Assuntos
Antioxidantes/química , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/farmacologia , Micélio/química , Pleurotus/química , Acetilação , Animais , Antioxidantes/farmacologia , Biomarcadores , Peso Corporal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Camundongos , Tamanho do Órgão/efeitos dos fármacos , Análise Espectral
5.
Int J Biol Macromol ; 131: 315-322, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-30872051

RESUMO

The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated. Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures. ERPS can improve liver fibrosis by reducing the level of pivotal cytokine TGF-ß1. Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway. Furthermore, the structural characteristics demonstrated that the ERPS was a typical ß-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA. These results demonstrated that the ERPS might be suitable for functional foods and natural drugs for preventing the acute liver damage.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/farmacologia , Pleurotus/química , Substâncias Protetoras/química , Substâncias Protetoras/farmacologia , Animais , Biomarcadores , Cromatografia Líquida de Alta Pressão , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Camundongos , Monossacarídeos/análise , Análise Espectral
6.
Int J Biol Macromol ; 129: 281-292, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30708002

RESUMO

In the present work, we investigated the anti-hyperlipidemic, antioxidant and organic protection effects of acidic-extractable Dictyophora indusiata polysaccharides (Ac-DPS) on hyperlipidemic mice induced by high-fat emulsion. The results demonstrated that Ac-DPS had impressive abilities to mitigate oxidative stress by increasing the activities of antioxidant enzyme and reducing the contents of lipid peroxide. Moreover, lipid levels in serum were returned to normal status. Besides, Ac-DPS exhibited potential hepatic and renal protection effects reflected by decreasing serum enzyme activities, lowering TBIL, UREA and CREA levels and increasing ALB content. At the same time, histopathological observations proved protective effects of Ac-DPS on organs. Subsequently, the physical properties of polysaccharide were also investigated by HPGPC and FT-IR. The above consequences confirmed the important role of Ac-DPS as a functional food and natural medicine in the fight against oxidative stress and the prevention of hyperlipidemia.


Assuntos
Antioxidantes/farmacologia , Basidiomycota/química , Polissacarídeos Fúngicos/farmacologia , Hipolipemiantes/farmacologia , Substâncias Protetoras/farmacologia , Animais , Antioxidantes/química , Biomarcadores , Polissacarídeos Fúngicos/química , Hipolipemiantes/química , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Masculino , Camundongos , Peso Molecular , Substâncias Protetoras/química , Análise Espectral
7.
Int J Biol Macromol ; 129: 316-325, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30708004

RESUMO

The aims of this work were to extract the enzymatic residue polysaccharides (EnRPS) from Pleurotus ostreatus and investigate the antioxidant and anti-hyperlipidemic effects on high-fat-high-cholesterol emulsion (HFHCE)-induced liver injured mice. The results revealed that EnRPS at 400 mg/kg bw showed superior liver protective effects by ameliorating the AI, lowing the hepatic MPO, FFA, ADPN, TC and TG indicators, and improving the antioxidant status by enhancing liver enzyme activities (SOD, CAT, LEP, INS and T-AOC), eliminating the MDA content, decreasing the levels of LDL-C, TC, TG, ALT, AST and ALP, and increasing the HDL-C in serum. And in addition, GC, FTIR, NMR and pathological sections were also studied. The above consequences suggested that EnRPS could be used as functional foods of oxidative stress and anti-hyperlipidemic against liver injury.


Assuntos
Polissacarídeos Fúngicos/farmacologia , Hipolipemiantes/farmacologia , Pleurotus/química , Animais , Antioxidantes/química , Antioxidantes/farmacologia , Biomarcadores , Polissacarídeos Fúngicos/química , Teste de Tolerância a Glucose , Hipolipemiantes/química , Metabolismo dos Lipídeos/efeitos dos fármacos , Lipídeos/sangue , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Masculino , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Análise Espectral , Testes de Toxicidade Aguda
8.
Int J Biol Macromol ; 127: 476-485, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30659878

RESUMO

A modified polysaccharide named MPCC from Coprinus comatus (MPCC) was obtained by the snailase hydrolysis. And the hepatoprotective effects on alcohol-induced liver injury and preliminary structure features were investigated. For in vivo hepatoprotective abilities, MPCC significantly attenuated the hepatic and serum lipid levels, obviously enhanced antioxidant enzyme activities, markedly improved alcohol metabolism system and inflammatory response, and mitigated alcohol-induced liver injury histopathologically, providing references for the exploitation of MPCC as functional foods or natural drugs against the alcohol-induced liver injury. Additionally, MPCC containing fucose (Fuc), ribose (Rib), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with the a α- and ß-configuration in a percentage composition of 0.91%, 0.71%, 0.45%, 1.60%, 2.04%, 4.41% and 89.88% via gas chromatography (GC), Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analysis.


Assuntos
Antioxidantes , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Coprinus/química , Etanol/efeitos adversos , Fígado/metabolismo , Polissacarídeos , Animais , Antioxidantes/química , Antioxidantes/farmacologia , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Etanol/farmacologia , Fígado/patologia , Camundongos , Polissacarídeos/química , Polissacarídeos/farmacologia
9.
Carbohydr Polym ; 201: 75-86, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30241865

RESUMO

The present work investigated the antioxidant and hepatoprotective effects of acidic- and enzymatic-hydrolysis mycelium polysaccharide (AcMPS and EnMPS) from Pleurotus geesteranus on alcoholic liver disease (ALD) mice. The animal studies demonstrated that the polysaccharides had potential effects reflected by remitting alcoholic hepatitis, reducing lipid accumulation, preventing oxidative stress, improving inflammatory symptoms, and alleviating the liver functions by histopathologic observation. Results showed that AcMPS (yield of 84%) was composed of L-Rha, D-Rib, L-Ara, D-Glc, D-Man and D-Gal with the Mw of 3.49 × 104 Da, while EnMPS (yield of 90%) was contained L-Rha, L-Ara, D-Gal and D-Glc with the Mw of 3.67 × 104 Da. Furthermore, the GC-MS analysis indicated that both AcMPS and EnMPS were ß-pyranoside polysaccharides with the (1→3)- and (1→6)-linkages. The conclusions indicated that AcMPS and EnMPS could be used as natural drugs for preventing the ALD, and providing underlying hepatoprotective mechanisms, pharmaceutically.


Assuntos
Antioxidantes/química , Polissacarídeos Fúngicos/química , Derivados de Hidroxietil Amido/química , Hepatopatias Alcoólicas/tratamento farmacológico , Micélio/enzimologia , Pleurotus/enzimologia , Soroalbumina Bovina/química , Animais , Antioxidantes/uso terapêutico , Bovinos , Modelos Animais de Doenças , Polissacarídeos Fúngicos/uso terapêutico , Hidrólise , Derivados de Hidroxietil Amido/uso terapêutico , Masculino , Camundongos
10.
Sci Rep ; 8(1): 11695, 2018 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-30076364

RESUMO

The aim of this work was to provide a preliminary characterization of alkalic-extractable polysaccharides (ALPS) from Coprinus comatus, to explore its in vivo antioxidant activities and protective effects on alcohol-induced liver injury. ALPS showed strong antioxidant and anti-inflammatory abilities and markedly low serum enzyme activities, hepatic and serum lipid levels, as well as low hepatic lipid peroxidation levels; moreover, ALPS improved the alcohol metabolism system. These results were also confirmed by an analysis of histopathological section observations. ALPS, in both α- and ß-configurations, as analysed by fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR), was mainly composed of rhamnose (Rha), fucose (Fuc), ribose (Rib), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with mass percentages of 0.52%, 1.02%, 0.80%, 0.92%, 3.05%, 2.96% and 90.73%, respectively. These results may offer support for the use of ALPS as a functional food or natural drug source that can prevent and treat alcohol-induced liver injury.


Assuntos
Álcalis/química , Antioxidantes/uso terapêutico , Coprinus/química , Etanol/toxicidade , Fígado/lesões , Polissacarídeos/uso terapêutico , Alanina Transaminase/sangue , Fosfatase Alcalina/sangue , Animais , Antioxidantes/farmacologia , Aspartato Aminotransferases/sangue , Colesterol/sangue , Citocinas/metabolismo , Etanol/administração & dosagem , Mediadores da Inflamação/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/patologia , Camundongos , Polissacarídeos/farmacologia , Testes de Toxicidade Aguda , Triglicerídeos/sangue
11.
Sci Rep ; 8(1): 10493, 2018 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-30002448

RESUMO

The present work investigated the hepatoprotective role of exopolysaccharides (EPS) isolated from the mushroom Pleurotus geesteranus with respect to alcohol-induced liver injury in mice. Based on a physico-chemical analysis, the EPS produced by Pleurotus geesteranus was identified as a heteropolysaccharide with α-glycosidic bond. The results revealed that prophylactic application of the EPS reduces detrimental alcoholic effects on the liver. This observation was followed by decreased levels of total cholesterol, triglycerides, CYP2E1 and pro-inflammatory mediators (TNF-α, IL-6, IL-1ß, COX-2, NO and iNOS) in the liver homogenates, suggesting that the EPS exhibits anti-inflammatory and hepatoprotective effects. Moreover, the increased activity of hepatic enzymes (superoxide dismutase, glutathione peroxidase and catalase) and reduced lipid peroxidation status indicated that the antioxidative effect of the EPS contributes to alleviation of liver injury. Therefore, this study reports that the EPS produced by Pleurotus geesteranus could be considered a potential natural drug or functional food supplement for the prevention of liver damage.


Assuntos
Anti-Inflamatórios/administração & dosagem , Antioxidantes/administração & dosagem , Polissacarídeos Fúngicos/administração & dosagem , Hepatopatias Alcoólicas/prevenção & controle , Pleurotus/química , Administração Oral , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Modelos Animais de Doenças , Etanol/administração & dosagem , Etanol/toxicidade , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/isolamento & purificação , Humanos , Injeções Intraperitoneais , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Hepatopatias Alcoólicas/diagnóstico , Hepatopatias Alcoólicas/etiologia , Hepatopatias Alcoólicas/patologia , Testes de Função Hepática , Masculino , Camundongos , Estresse Oxidativo/efeitos dos fármacos
12.
Sci Rep ; 8(1): 7574, 2018 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-29765084

RESUMO

The present work was designed to evaluate the antioxidation and hepatoprotective effects of Auricularia cornea var. Li. polysaccharides (APS) and enzymatic-extractable APS (EAPS) on the acute alcohol-induced alcoholic liver diseases (ALD). The in vitro antioxidant activities demonstrated that both APS and EAPS had strong reducing power and potential effects on scavenging reactive oxygen species. The in vivo mice experiments showed that the pretreatment with APS or EAPS showed potential hepatoprotective effects on the ALD possibly by increasing the antioxidant activities, reducing the lipid peroxidation, improving the alcohol metabolism, inhibiting the expression levels of inflammatory mediators and preventing the alcohol-induced histopathological alterations. In addition, the fourier-transform infrared (FT-IR), 1H and 13C nuclear magnetic resonance spectroscopy (NMR) and gas chromatography (GC) had been analyzed to obtained the primarily characteristics. The results indicated that abundant xylose and glucose contents probably had potential effects on possessing the bioactivities. The findings suggested that the A. cornea var. Li. might be considered as promising natural resource on exploring clinical drugs for the prevention and treatment with ALD and its complications.


Assuntos
Agaricales/química , Antioxidantes/administração & dosagem , Polissacarídeos Fúngicos/administração & dosagem , Hepatopatias Alcoólicas/tratamento farmacológico , Animais , Antioxidantes/química , Antioxidantes/farmacologia , Cromatografia Gasosa , Modelos Animais de Doenças , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/farmacologia , Glucose/isolamento & purificação , Glucose/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Hepatopatias Alcoólicas/metabolismo , Masculino , Camundongos , Espectroscopia de Prótons por Ressonância Magnética , Espécies Reativas de Oxigênio/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Resultado do Tratamento , Xilose/isolamento & purificação , Xilose/farmacologia
13.
Oxid Med Cell Longev ; 2018: 9150807, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30595798

RESUMO

The present work was performed to evaluate the effect of acidic-extractable polysaccharides (AE-PS) from fruit bodies of Cordyceps militaris on type 2 diabetes mellitus (T2DM) and its structural characteristics. The T2DM mice induced by high-fat diet (HFD) and streptozotocin (STZ) were administered with 100 and 400 mg/kg AE-PS for 4 weeks. Our work proved that AE-PS decreased the levels of serum lipid, lipid peroxidation, and blood glucose; improved glucose and insulin resistance; enhanced antioxidant enzyme activities; and attenuated the injuries of the liver, kidney, and pancreas in T2DM mice. These results might offer references for the exploitation of AE-PS as functional foods or natural drug source for preventing and treating HFD- and STZ-induced T2DM. Moreover, gas chromatography (GC) results revealed that AE-PS was heterogeneous and composed of fucose, ribose, arabinose, xylose, mannose, galactose, and glucose with mass percentages of 1.23%, 0.57%, 0.29%, 2.12%, 2.73%, 4.66%, and 88.4%, respectively. Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) analysis indicated that AE-PS was a pyran-type polysaccharide with α- and ß-configurations.


Assuntos
Cordyceps/química , Diabetes Mellitus Experimental/tratamento farmacológico , Hipoglicemiantes/farmacologia , Animais , Antioxidantes , Modelos Animais de Doenças , Camundongos
14.
Carbohydr Polym ; 175: 282-292, 2017 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-28917868

RESUMO

In this study, we noted that the Al-MPS from Pleurotus eryngii var. tuolensis provoked pharmacological effects on blood lipid profiles and oxidative stress. Animal studies demonstrated that Al-MPS showed potential effects on relieving hyperlipidemia and preventing oxidative stress, reflecting by decreasing the levels of serum enzyme activities (ALP, ALT and AST), restoring the activities of antioxidant enzymes (SOD, GSH-Px, CAT and T-AOC), down-regulating the MDA and LPO contents, as well as remitting the hepatic and cardiac tissues injury, respectively. The serum levels of TC, TG, LDL-C, VLDL-C, and HDL-C on mice treated with Al-MPS (500mg/kg bw) reached 2.48±0.08, 1.24±0.03, 0.84±0.02, 0.34±0.02, and 1.80±0.03mmol/L, which were lower/higher against the hyperlipidemia mice. The results clearly indicated that the Al-MPS could be used as a beneficial health food and potentially natural candidate medicine in preventing the high-fat emulsion-induced hyperlipidemia.


Assuntos
Antioxidantes/farmacologia , Hipolipemiantes/farmacologia , Estresse Oxidativo , Pleurotus/química , Polissacarídeos/farmacologia , Álcalis , Animais , Coração , Fígado , Masculino , Camundongos , Micélio/química
15.
Sci Rep ; 7(1): 3910, 2017 06 20.
Artigo em Inglês | MEDLINE | ID: mdl-28634377

RESUMO

In this study, native depolymerised-exopolysaccharides (DEPS) were successfully derived from the exopolysaccharides (EPS) of Termitomyces albuminosus, and its hepatoprotective effects against a high-fat emulsion and in vitro antioxidant activities were investigated. Based on the results of in vitro assays, DEPS showed superior antioxidant activities compared with EPS dose-dependently. According to the in vivo assays both EPS and DEPS significantly decreased the lipid levels, improved the enzymatic activities, and reduced lipid peroxidation in both serum and hepatic homogenates. Furthermore, EPS and DEPS attenuated the high-fat emulsion-induced histopathological injury to the liver. Both EPS and DEPS might be used as natural drugs to treat and protect against hyperlipidaemia and liver injury induced by a high-fat emulsion. In addition, based on the results of GC and HPLC analyses, rhamnose and low molecular weight compounds may play an important role in contributing to the antioxidant activities of EPS and DEPS.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/farmacologia , Substâncias Protetoras/química , Substâncias Protetoras/farmacologia , Termitomyces/química , Animais , Biomarcadores , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Lipídeos/sangue , Fígado/efeitos dos fármacos , Fígado/metabolismo , Camundongos
16.
Int J Biol Macromol ; 102: 351-357, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28412338

RESUMO

The in vitro antioxidant and in vivo anti-aging activities in D-galactose-induced aging mice of exopolysaccharides (EPS) and its fractions (EPS-1, EPS-2 and EPS-3) by Agrocybe cylindraceas were investigated presently. The scavenging effects of EPS-2 on hydroxyl (HO) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 97.1±4.25% and 90.23±3.25%, while the reducing power reached 1.18±0.08 at 120mg/L, which were higher than EPS, EPS-1 and EPS-3 at 1000mg/L. The EPS showed potential anti-aging activities via reducing the contents of malonaldehyde (MDA) and total cholesterol (TC), as well as increasing the activities of superoxide dismutase (SOD) activities, glutathione peroxidase (GSH-Px) activities, and total antioxidant capacity (T-AOC). Additionally, the monosaccharide analysis showed EPS contained rhamnose and glucose with the molar ratio of 2.8:1. These results demonstrated that EPS could be used as functional foods and natural drugs on preventing and mitigating the aging process.


Assuntos
Envelhecimento/efeitos dos fármacos , Agrocybe/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Polissacarídeos Fúngicos/isolamento & purificação , Polissacarídeos Fúngicos/farmacologia , Animais , Antioxidantes/química , Compostos de Bifenilo/química , Polissacarídeos Fúngicos/química , Radical Hidroxila/química , Masculino , Camundongos , Monossacarídeos/análise , Picratos/química
17.
Oxid Med Cell Longev ; 2017: 5863523, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29430281

RESUMO

In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus. Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals. In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation. Furthermore, monosaccharide composition analysis showed that fucose may play a vital role in guaranteeing stronger hepatoprotection. These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.


Assuntos
Agaricales/química , Antioxidantes/uso terapêutico , Hepatopatias Alcoólicas/tratamento farmacológico , Polissacarídeos/metabolismo , Animais , Antioxidantes/farmacologia , Hepatopatias Alcoólicas/patologia , Camundongos
18.
Carbohydr Polym ; 151: 1227-1234, 2016 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-27474674

RESUMO

Two polysaccharides, EIPS and AIPS were obtained by the hydrolysis of IPS from Termitomyces albuminosus, and their pharmacological effects on blood lipid profiles metabolism and oxidative stress were investigated. The results demonstrated that EIPS was superior to IPS and AIPS on reducing hepatic lipid levels and preventing oxidative stress by improving serum enzyme activities (ALT, AST, and ALP), serum lipid levels (TC, TG, HDL-C, LDL-C and VLDL-C), hepatic lipid levels (TC and TG), and antioxidant status (SOD, GSH-Px, CAT, T-AOC, MDA, and LPO). These conclusions indicated that EIPS, AIPS and IPS might be suitable for functional foods and natural drugs on preventing the high-fat emulsion-induced hyperlipidemia. In addition, the monosaccharide compositions of IPS and its hydrolyzate were also processed.


Assuntos
Enzimas/metabolismo , Hipolipemiantes/farmacologia , Espaço Intracelular/química , Polissacarídeos/farmacologia , Termitomyces/citologia , Antioxidantes/metabolismo , Peso Corporal/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Hidrólise , Hipolipemiantes/química , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Malondialdeído/metabolismo , Monossacarídeos/análise , Estresse Oxidativo/efeitos dos fármacos , Polissacarídeos/química
19.
Curr Microbiol ; 73(3): 379-385, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27262461

RESUMO

The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work. By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137. The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group. Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved. In liver tissue, the lipid peroxidation and malondialdehyde were largely decreased, and the superoxide dismutase and catalase activities were significantly increased. The evidence demonstrated that the EPS and IPS of H. erinaceus SG-02 were protective for liver injury. The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.


Assuntos
Basidiomycota/química , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Fígado/efeitos dos fármacos , Polissacarídeos/administração & dosagem , Substâncias Protetoras/administração & dosagem , Alanina Transaminase/metabolismo , Animais , Aspartato Aminotransferases/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Colesterol/sangue , Humanos , Peroxidação de Lipídeos , Fígado/metabolismo , Masculino , Malondialdeído/metabolismo , Camundongos , Polissacarídeos/química
20.
Carbohydr Polym ; 146: 388-95, 2016 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-27112888

RESUMO

Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated. Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently. Furthermore, the histopathological observations also displayed that En-RPS could alleviate kidney damage. These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy. In addition, the monosaccharide compositions of these three RPS were also analyzed.


Assuntos
Antioxidantes/farmacologia , Flammulina/química , Polissacarídeos Fúngicos/farmacologia , Animais , Rim/efeitos dos fármacos , Masculino , Camundongos , Substâncias Protetoras/farmacologia
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