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1.
Alcohol Alcohol ; 2022 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-34999760

RESUMO

INTRODUCTION: Managed Alcohol Programs (MAPs) are designed to improve health and housing outcomes for unstably housed people with an alcohol use disorder (AUD). The present study assesses the association of MAP participation with healthcare and mortality outcomes. METHODS: A retrospective cohort study assessed health outcomes for 205 MAP participants and 128 controls recruited from five Canadian cities in 2006-2017. Survival and negative binomial regression models were used to calculate hazard ratios (HR) of death and emergency room (ER) visits and hospital bed days (HBDs). Covariates included age, sex, AUD severity and housing stability score. RESULTS: In fully adjusted models, compared with times outside MAPs, participants had significantly reduced risk of mortality (HR = 0.37, P = 0.0001) and ER attendance (HR = 0.74, P = 0.0002), and fewer HBDs yearly (10.40 vs 20.08, P = 0.0184). Over the 12 years, people enrolled in a MAP at some point had significantly fewer HBDs per year than controls after MAP enrolment (12.78 vs 20.08, P = 0.0001) but not significantly different rates of death or ER presentation. MAP participants had significantly more alcohol-related but significantly fewer nonalcohol-related ER presentations than controls. CONCLUSION: Attendance at a MAP was associated with reduced risk of mortality or morbidity and less hospital utilization for individuals with unstable housing and severe AUDs. MAPs are a promising approach to reduce mortality risk and time spent in hospital for people with an AUD and experiencing homelessness.

2.
J Environ Manage ; 301: 113793, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34601347

RESUMO

Municipal wastewater treatment plants (WWTPs) have been regarded as the main receptors of microplastics in industrial and domestic wastewater. The excess sludge they generate is an important carrier for the microplastics to enter the environment. In China, relevant regional studies are still in an initial phase. In this work, microplastics in the sewage sludges at different sampling points of five WWTPs in Nanjing City (an important city in the Yangtze River basin) were investigated, including their abundance, morphology and chemical composition. Furthermore, the influence factors such as population density, economic development level, wastewater source and treatment process were also discussed. The analysis results through optical microscope and FT-IR showed that the detected microplastics were divided into fragments, films, fibers and granules. Their chemical component reached up to 19 species, including small amounts of petroleum resins which was scarcely detected in other studies. Wastewater source was the primary factor influencing the microplastic abundance and size in sludge. And the microplastic shape and chemical components were closely related to the industrial type. Furthermore, because the removal effect on the microplastics with different morphologies were varied with the treatment process, the preliminary suggestions on the technology for particular wastewater were proposed. This study provides partial regional data and analysis for the microplastics contained in the sludge of WWTPs, expecting to provide a certain theoretical support for the operations management of WWTPs and standardized sludge treatment.


Assuntos
Poluentes Químicos da Água , Purificação da Água , China , Monitoramento Ambiental , Microplásticos , Plásticos , Esgotos , Espectroscopia de Infravermelho com Transformada de Fourier , Eliminação de Resíduos Líquidos , Águas Residuárias/análise , Poluentes Químicos da Água/análise
3.
PeerJ Comput Sci ; 7: e635, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34604513

RESUMO

Concrete is the main material in building. Since its poor structural integrity may cause accidents, it is significant to detect defects in concrete. However, it is a challenging topic as the unevenness of concrete would lead to the complex dynamics with uncertainties in the ultrasonic diagnosis of defects. Note that the detection results mainly depend on the direct parameters, e.g., the time of travel through the concrete. The current diagnosis accuracy and intelligence level are difficult to meet the design requirement for automatic and increasingly high-performance demands. To solve the mentioned problems, our contribution of this paper can be summarized as establishing a diagnosis model based on the GA-BPNN method and ultrasonic information extracted that helps engineers identify concrete defects. Potentially, the application of this model helps to improve the working efficiency, diagnostic accuracy and automation level of ultrasonic testing instruments. In particular, we propose a simple and effective signal recognition method for small-size concrete hole defects. This method can be divided into two parts: (1) signal effective information extraction based on wavelet packet transform (WPT), where mean value, standard deviation, kurtosis coefficient, skewness coefficient and energy ratio are utilized as features to characterize the detection signals based on the analysis of the main frequency node of the signals, and (2) defect signal recognition based on GA optimized back propagation neural network (GA-BPNN), where the cross-validation method has been used for the stochastic division of the signal dataset and it leads to the BPNN recognition model with small bias. Finally, we implement this method on 150 detection signal data which are obtained by the ultrasonic testing system with 50 kHz working frequency. The experimental test block is a C30 class concrete block with 5, 7, and 9 mm penetrating holes. The information of the experimental environment, algorithmic parameters setting and signal processing procedure are described in detail. The average recognition accuracy is 91.33% for the identification of small size concrete defects according to experimental results, which verifies the feasibility and efficiency.

4.
Mater Sci Eng C Mater Biol Appl ; 130: 112461, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34702536

RESUMO

Laser powder bed fusion (LPBF) additive manufacturing of pure tantalum and their graded lattice structures was systematically investigated, with emphasis on their microstructure evolution, phase formation, surface energy and biological properties in comparison with conventionally forged pure Ta. The LPBF fabricated Ta (LPBF-Ta) exhibited lower contact angles and higher surface energy than the forged-Ta which indicated the better wettability of the LPBF-Ta. The adhesion and proliferation of rat bone marrow stromal cells (rBMSCs) were also enhanced for the LPBF-Ta when compared to forged-Ta. Three different Ta graded gyroid lattice structures (i.e., uniform structure, Y-gradient structure, Z-gradient structure) were designed and fabricated using the same optimised LPBF parameters. Y-gradient structures exhibited the best plateau stress and compressive modulus among three different graded structures due to the maximum local volume fraction on the fracture plane. In fatigue response, Y-gradient outperformed the other two gyroid structures under varying stresses. In terms of cell culture response, the uniform structures performed the best biocompatibility due to its suitable pore size for cell adhesion and growth. This study provides new and in-depth insights into the LPBF additive manufacturing of pure Ta graded lattice structures with desired fatigue and biological properties for load-bearing orthopaedic applications.


Assuntos
Ortopedia , Tantálio , Animais , Teste de Materiais , Porosidade , Ratos , Suporte de Carga
5.
Sensors (Basel) ; 21(19)2021 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-34640924

RESUMO

The development and research of an ultrasonic-based concrete structural health monitoring system encounters a variety of problems, such as demands of decreasing complexity, high accuracy, and extendable system output. Aiming at these requirements, a low-cost extendable system based on FPGA with adjustable system output has been designed, and the performance has been evaluated by different assessment parameters set in this paper. Besides the description of the designed system and the experiments in air medium, the residual similarity and Pearson correlation coefficients of experimental and theoretical data have been used to evaluate the submodules' output. The output performance of the overall system is evaluated by the Pearson correlation coefficient, root-mean-square error (RMSE), and magnitude-squared coherence with 40 experimental data. The maximum, median, minimum, and mean values in three-parameter datasets are analyzed for discussing the working condition of the system. The experimental results show that the system works stably and reliably with tunable frequency and amplitude output.


Assuntos
Eletrocardiografia , Ultrassom , Monitorização Fisiológica
6.
Planta ; 254(4): 79, 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34542712

RESUMO

MAIN CONCLUSION: HDA704 enhances drought and salt tolerance via stomata-regulated mechanism. HDA704 negatively regulates stomatal aperture and density, repressing the transcription of DST and ABIL2 by histone deacetylation modification. Drought and salinity can damage crop growth and reduce yield. Stomata play an important role in abiotic stress tolerance. In this study on rice, we identified the RPD3/HDA1-type histone deacetylase HDA704 as a positive regulatory factor in drought and salt tolerance. HDA704 was induced by drought and salt stresses. Overexpression of HDA704 in transgenic rice promoted stomatal closure, decreased the number of stomata and slowed down the rate of water loss, consequently resulting in increased drought and salt tolerance. By contrast, knockdown of HDA704 in transgenic rice decreased stomatal closure and accelerated the rate of water loss, leading to decrease drought and salt tolerance. We detected the transcript expression of DST (Drought and Salt Tolerance) and ABIL2 (Abscisic Acid-insensitive Like2), which positively regulate stomatal aperture and density in rice. Our results showed that HDA704 directly binds to DST and ABIL2, repressing their expression via histone deacetylation modification. Collectively, these findings reveal that HDA704 positively regulates drought and salt tolerance by repressing the expression of DST and ABIL2. Our findings provide a new insight into the molecular mechanisms of stomata-regulated abiotic stress tolerance of plants.


Assuntos
Oryza , Ácido Abscísico , Secas , Regulação da Expressão Gênica de Plantas , Histona Desacetilases/genética , Oryza/genética , Oryza/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estômatos de Plantas/genética , Estômatos de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Tolerância ao Sal , Estresse Fisiológico
7.
Poult Sci ; 100(10): 101378, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34391174

RESUMO

Rapid detection of antibiotic residues in duck meat is of great significance for strengthening food safety and quality supervision of duck meat and fighting against inferior products in the duck meat market. The objective of the current paper was to evaluate the potential of synchronous fluorescence spectroscopy (SFS) coupled with chemometric methods for the rapid detection of sulfamethazine (SM2) and ofloxacin (OFL) residues in duck meat.The SFS spectral data from duck meat containing different concentrations of SM2 and OFL were preprocessed by baseline offset. The detection conditions, including the adding amounts of ß-mercaptoethanol solution and o-phthalaldehyde solution, as well as the reaction time, were optimized by a single factor experiment for obtaining a better detection effect, and their optimal values were 400 µL , 25 µL , and 40 min, respectively. By comparing 2 chemometric models based on peak-height algorithm and peak-area algorithm, the prediction model based on peak-height algorithm was a better quantitative model with correlation coefficient for the prediction set (Rp) of 0.9031 and 0.9981, the root mean error for the prediction set (RMSEP) of 7.9509 and 0.5267 mg/kg, recovery of 81.7 to 155.1% and 96.4 to 111.2%, and relative standard deviation (RSD) of 4.1 to 6.7% and 2.9 to 6.8% to predict SM2 and OFL residues in duck meat, respectively. Overall, the results of this investigation showed that SFS technique was an effective and rapid tool for the detection of SM2 and OFL residues in duck meat.


Assuntos
Ofloxacino , Sulfametazina , Animais , Galinhas , Patos , Carne/análise , Espectrometria de Fluorescência/veterinária
8.
Rapid Commun Mass Spectrom ; 35(17): e9149, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34156734

RESUMO

RATIONALE: Low-molecular-weight organic acids that generally contain one to three carboxyl groups are involved in many important biological processes; therefore, it is important to develop a quantitative method for analyzing organic acids in serum in order to allow an evaluation of metabolic changes. In this study, we evaluated a protocol for detecting 26 organic acids in serum based on ultrasound-assisted derivatization by gas chromatography/mass spectrometry (GC/MS). METHODS: Serum samples were prepared using ultrasound-assisted silane derivatization before GC/MS analysis to quantify concentrations of organic acids. Additionally, we investigated the variables affecting derivatization yields, including the extraction solvent, derivatization reagents, and derivatization conditions (reaction temperature, duration, and sonication parameters). The protocol was ultimately applied to detect organic acid profiles related to obesity. RESULTS: We used acetone as the extraction solvent and determined suitable derivatization conditions, as follows: BSTFA + 1% TMCS, 50°C, 10 min, and 100% ultrasound power. The protocol showed satisfactory linearity (r = 0.9958-0.9996), a low limit of detection (0.04-0.42 µmol/L), good reproducibility (coefficient of variation (CV) %: 0.32-13.76%), acceptable accuracy (recovery: 82.97-114.96%), and good stability within 5 days (CV%: 1.35-12.01% at room temperature, 1.24-14.09% at 4°C, and 1.01-11.67% at -20°C). Moreover, the protocol was successfully applied to obtain the organic acid profiles from obese and healthy control subjects. CONCLUSIONS: We identified and validated a protocol for ultrasound-assisted derivatization prior to GC/MS analysis for detecting 26 kinds of organic acids in serum. The results suggest the efficacy of this protocol for clinical applications to determine metabolic changes related to fluctuations in organic acid profiles.

9.
Poult Sci ; 100(6): 101165, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33975036

RESUMO

This paper investigated on 478 duck meat samples for the identification of 2 kinds of antibiotics, that is, doxycycline hydrochloride and tylosin, that were classified based on surface-enhanced Raman spectroscopy (SERS) combined with multivariate techniques. The optimal detection parameters, including the effects of the adsorption time, and 2 enhancement substrates (i.e., gold nanoparticles as well as gold nanoparticles and NaCl) on Raman intensities, were analyzed using single factor analysis method. The results showed that the optimal adsorption time between gold nanoparticles and analytes was 2 min, and the colloidal gold nanoparticles without NaCl as the active substrate were more conducive to enhance the Raman spectra signal. The SERS data were pretreated by using the method of adaptive iterative penalty least square method (air-PLS) and second derivative, and from which the feature vectors were extracted with the help of principal component analysis. The first four principal components scores were selected as the input values of support vector machines model. The overall classification accuracy of the test set was 100%. The experimental results showed that the combination of SERS and multivariate analysis could identify the residues of doxycycline hydrochloride and tylosin in duck meat quickly and sensitively.


Assuntos
Nanopartículas Metálicas , Análise Espectral Raman , Animais , Galinhas , Doxiciclina , Patos , Ouro , Carne , Tilosina
10.
Orthop Surg ; 13(4): 1205-1212, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33942553

RESUMO

OBJECTIVE: To compare the accuracy of combined independent risk factors in assessing the risk of hip fractures in elderly women. METHODS: Ninety elderly females who sustained hip fractures (including femoral neck fractures and intertrochanteric fractures) and 110 female outpatients without a hip fracture were included in our cross-sectional study from 24 November 2017 to 20 May 2019. The age of subjects in the present study was ≥65 years, with the mean age of 78.73 ± 7.77 and 78.09 ± 5.03 years for women with and without elderly hip fractures, respectively. Bone mineral density (BMD), Beta-carboxy terminal telopeptide (ß-CTX), N-terminal/mid region (N-MID), and 25(OH)D levels were analyzed. A novel evaluation model was established to evaluate combined indicators in assessing hip fractures in elderly women. RESULTS: Compared with the control group, taller height (155.68 ± 6.40 vs 150.97 ± 6.23, P < 0.01), higher levels of ß-CTX (525.91 ± 307.38 vs 330.94 ± 289.71, P < 0.01), and lower levels of total hip BMD (0.662 ± 0.117 vs 0.699 ± 0.111, P = 0.022), femoral neck BMD (0.598 ± 0.106 vs 0.637 ± 0.100, P = 0.009), and 25(OH)D (15.67 ± 7.23 vs 29.53 ± 10.57, P < 0.01) were found in the facture group. After adjustment for confounding factors, logistic regression analysis revealed that 25(OH)D (adjusted OR 0.837 [95% CI 0.790-0.886]; P < 0.01), femoral neck BMD (adjusted OR 0.009 [95% CI 0.000-0.969]; P = 0.048) and height (adjusted OR 1.207 [95% CI 1.116-1.306]; P < 0.01) remained risk factors for hip fractures in elderly women. Then a model including independent risk factors was established. A DeLong test showed the area under the receiver operator characteristic (ROC) (Area under the curve [AUC]) of 25(OH)D was significantly greater than that for femoral neck BMD (P < 0.01) and height (P < 0.01). The AUC of model including 25(OH)D and height was significantly greater than that of other combinations (P < 0.01). CONCLUSION: 25(OH)D, femoral neck BMD and height were associated with the occurrence of hip fractures in elderly women even after adjustment for confounding factors, and a model including 25(OH)D and height could provide better associated power than other combinations in the assessment of elderly hip fractures.


Assuntos
Fraturas do Quadril/etiologia , Fraturas por Osteoporose/etiologia , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Densidade Óssea/fisiologia , Estudos Transversais , Feminino , Humanos , Incidência , Valor Preditivo dos Testes , Fatores de Risco
11.
J Pharm Biomed Anal ; 200: 114058, 2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-33865049

RESUMO

AIMS: The aim of this study was to identify novel serum metabolites associated with non-alcoholic fatty liver disease (NAFLD), and to explore the metabolic discrepancies between Lean-NAFLD and Obese-NAFLD. METHODS: Serum samples from patients with NAFLD (n = 161) and healthy participants (n = 149) were collected, and metabolites were analyzed with UPLC-Q-TOF MS/MS. Subgroup analyses were performed to explore the metabolic differences among Lean-NAFLD, Obese-NAFLD and healthy controls RESULTS: A total of 24 differentially present metabolites were found between patients with NAFLD and healthy controls. Marked metabolic pathway differences were observed among the NAFLD subtypes, including in fatty acid and amino acid metabolism. Ultimately, five metabolites (prasterone, indoxylsulfuric acid, sebacic acid, arachidonic acid and pregnenolone sulfate) were used to establish a diagnostic model to distinguish patients with NAFLD regardless of Lean- or Obese-NAFLD type. CONCLUSIONS: This study suggested that significant metabolic differences existed among subtypes of NAFLD, and our model might be useful to distinguish patients with NAFLD. These findings may lay a foundation for the detection and treatment of NAFLD subtypes.


Assuntos
Hepatopatia Gordurosa não Alcoólica , Humanos , Metabolômica , Hepatopatia Gordurosa não Alcoólica/diagnóstico , Obesidade , Espectrometria de Massas em Tandem
12.
Metabolomics ; 17(4): 38, 2021 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-33788045

RESUMO

BACKGROUND AND AIMS: Nephrotic syndrome (NS) is a common pediatric urinary system disease. The aim in this work was to investigate the changes in pediatric NS-related metabolites through serum metabolomics, and explore the new potential metabolites and differential metabolic pathways. METHODS: Serum samples from 40 pediatric patients with nephrotic syndrome and 40 healthy controls were collected. The targeted and non-targeted metabolomics analyses were performed to determine the metabolic changes in pediatric NS. Based on multivariate statistical analysis and the regression model, the serum potential metabolites were screened and different metabolic pathways were explored. RESULTS: 39 differential metabolites in pediatric NS were obtained based on the metabolomics analysis. 12 differential metabolites (serine, C18: 2 (EFA), C18: 2 (FFA), Isonuatigenin 3- [rhamnosyl- (1- > 2) -glucoside], C18: 4 (EFA), C18: 4 (FFA), caprylic acid, citric acid, methylmalonic acid, caproic acid, canavalioside and uroporphyrin were identified to establish the diagnostic model for pediatric NS. Five metabolic pathways including TCA cycle, amino acid metabolism, bile acid biosynthesis, linoleate metabolism and glyoxylate and dicarboxylate metabolism were the key differential metabolic pathways. CONCLUSION: These data elucidated the metabolic alterations associated with pediatric NS and suggested a new diagnosis model for monitoring pediatric NS. The current study provides the useful information to bridge the gaps in our understanding of the metabolic alterations associated with pediatric NS and might facilitate the characterization of pediatric NS patients by performing serum metabolomics.


Assuntos
Metabolômica/métodos , Síndrome Nefrótica/sangue , Aminoácidos/sangue , Aminas Biogênicas/sangue , Biomarcadores/sangue , Caprilatos , Criança , Pré-Escolar , Ácidos Graxos/sangue , Feminino , Humanos , Lactente , Masculino , Redes e Vias Metabólicas , Análise Multivariada
13.
Addiction ; 116(11): 3198-3205, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-33739484

RESUMO

AIMS: Building upon an existing methodology and conceptual framework for estimating the association between the use of substances and crime, we calculated attributable fractions that estimate the proportion of crimes explained by alcohol and six other categories of psychoactive substances. DESIGN: Cross-sectional surveys. SETTING: Canadian federal correctional institutions. PARTICIPANTS: Canadian men (n = 27 803) and women (n = 1335) offenders who began serving a custodial sentence in a Canadian federal correctional institution between 2006 and 2016. MEASUREMENTS: Offenders completed the computerized assessment of substance abuse, a self-report tool designed to assess (1) whether the offence for which they were convicted would have occurred had they not been intoxicated from alcohol or another substance, (2) whether they committed the offence to support their alcohol or other substance use and (3) whether they were dependent on alcohol (alcohol dependence scale) or another substance (drug abuse screening test). Offences were grouped into four mutually exclusive categories: violent crimes, non-violent crimes, impaired driving and substance-defined crimes. This study focused on violent and non-violent crime categories. Substances assessed were: alcohol, cannabis, opioids, other central nervous system (CNS) depressants, cocaine, other CNS stimulants and other substances. FINDINGS: According to offender self-report, 42% of all violent and non-violent crime would probably not have occurred if the perpetrator had not been under the influence of, or seeking, alcohol or other substances. Between 2006 and 2016, 20% of violent crimes and 7% of non-violent crimes in Canada were considered attributable to alcohol. In contrast, all other psychoactive substance categories combined were associated with 26% of all violent crime and 25% of non-violent crime during the same time-frame. CONCLUSIONS: Attributable fraction analyses show that more than 42% of Canadian crime resulting in a custodial sentence between 2006 and 2016 would probably not have occurred if the perpetrator had not been under the influence of or seeking alcohol or other drugs. Attributable fractions for alcohol and substance-related crime are a potentially useful resource for estimating the impact of alcohol and other substances on crime.


Assuntos
Condução de Veículo , Transtornos Relacionados ao Uso de Substâncias , Canadá/epidemiologia , Crime , Estudos Transversais , Humanos , Transtornos Relacionados ao Uso de Substâncias/epidemiologia
14.
Clin Chim Acta ; 518: 59-69, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33746017

RESUMO

BACKGROUND: Epithelial ovarian cancer (EOC) is a common gynecological cancer with high mortality rates. The main objective of this study was to investigate the serum amino acid and organic acid profiles to distinguish key metabolites for screening EOC patients. METHODS: In total, 39 patients with EOC and 31 healthy controls were selected as the training set. Serum amino acid and organic acid profiles were determined using the targeted metabolomics approach. Metabolite profiles were processed via multivariate analysis to identify potential metabolites and construct a metabolic network. Finally, a test dataset derived from 29 patients and 28 healthy controls was constructed to validate the potential metabolites. RESULTS: Distinct amino acid and organic acid profiles were obtained between EOC and healthy control groups. Methionine, glutamine, asparagine, glutamic acid and glycolic acid were identified as potential metabolites to distinguish EOC from control samples. The areas under the curve for methionine, glutamine, asparagine, glutamic acid and glycolic acid were 0.775, 0 778, 0.955, 0.874 and 0.897, respectively, in the validation study. Metabolic network analysis of the training set indicated key roles of alanine, aspartate and glutamate metabolism as well as D-glutamine and D-glutamate metabolism in the pathogenesis of EOC. CONCLUSIONS: Amino acid and organic acid profiles may serve as potential screening tools for EOC. Data from this study provide useful information to bridge gaps in the understanding of the amino acid and organic acid alterations associated with epithelial ovarian cancer.


Assuntos
Metabolômica , Neoplasias Ovarianas , Aminoácidos , Biomarcadores Tumorais , Carcinoma Epitelial do Ovário , Feminino , Glutamina , Humanos , Neoplasias Ovarianas/diagnóstico
15.
Adv Sci (Weinh) ; 8(6): 2004010, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33747747

RESUMO

Implant-associated infections (IAIs) are a common cause of orthopedic surgery failure due to microbial biofilm-induced antibiotic-resistance and innate immune inactivation. Thus, the destruction of microbial biofilm plays a key role in reducing IAIs. Herein, first, a magneto-based synergetic therapy (MST) is proposed and demonstrated against IAIs based on biofilm destruction. Under an alternating magnetic field (AMF), CoFe2O4@MnFe2O4 nanoparticles (MNPs), with a rather strong magnetic hyperthermal capacity, can generate sufficient thermal effect to cause dense biofilm dispersal. Loosened biofilms provide channels through which nitrosothiol-coated MNPs (MNP-SNOs) can penetrate. Subsequently, thermosensitive nitrosothiols rapidly release nitric oxide (NO) inside biofilms, thus efficiently killing sessile bacteria under the magnetothermal effect of MNPs. More importantly, MNP-SNOs can trigger macrophage-related immunity to prevent the relapse of IAIs by exposing the infected foci to a consistent innate immunomodulatory effect. The notable anti-infection effect of this nanoplatform is also confirmed in a rat IAI model. This work presents the promising potential of combining magnetothermal therapy with immunotherapy, for the effective and durable control and elimination of IAIs.

16.
Rapid Commun Mass Spectrom ; 35(7): e9042, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33395499

RESUMO

RATIONALE: The matrix effect is tricky in gas chromatography/mass spectrometry (GC/MS) analyses. Although several methods have been proposed to solve this problem, the results were unsatisfactory. Even fewer studies have assessed the performance of corrective methods. Hence, our study focused on assessing several common corrective methods, and then proposed a new strategy to correct for the matrix effect in GC/MS analyses. METHODS: In GC/MS analyses, the internal standard method (ISM) was employed to overcome the matrix effect during the detection of pyruvic acid (PA) in serum samples from a healthy adult female. The accuracy of the ISM was evaluated by comparing it with the standard addition method (SAM). To employ the ISM-SAM strategy, correction factors (CFs) were established by combining the ISM and the SAM based on different groups. The CFs were used to normalize data onto the results of subsequent analyses. RESULTS: When using the ISM to detect levels of PA, a serious bias is observed, thereby affecting the conclusions reached. In contrast, more reliable data can be obtained after normalizing results by undertaking the ISM-SAM strategy. The feasibility of this strategy was verified by comparing it with the results of the SAM alone. The ISM-SAM strategy was successfully applied to quantify the PA levels in healthy people and nephrotic syndrome patients. CONCLUSIONS: Our results indicated that a false outcome was presented when only the ISM was used to adjust the data, and important information would be missed if the correction strategy was not carried out. Therefore, ISM-SAM, as an available correction method, should be adapted to improve the reliability of research results.

17.
Environ Sci Pollut Res Int ; 28(8): 9327-9337, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33141384

RESUMO

Municipal wastewater treatment plants (WWTPs) are considered to be major contributors of microplastics to the aquatic environment. Detailed research in China, which is relevant to the local situation, remains in the initial stage. Herein, the microplastic abundance, morphology, and removal efficiency of two WWTPs (C and P) equipped with tertiary treatment processes in different districts of Nanjing, an important city in the Yangtze River Basin, were investigated. The influence of technology, operational parameters, daily capacity, and sewage source and its proportion were discussed. Observations by optical microscope and FT-IR analysis and systematic calculation revealed that the microplastics have four shapes, including fragments, granules, film, and fibers, with various sizes and proportions, which were dependent on wastewater source. The total removal rates of 97.67% and 98.46% for WWTP C and WWTP P, respectively, indicated their highly efficient reduction of microplastics. Treatment technology had a considerable influence on the removal rate, especially the secondary and tertiary processes. However, a large number of microplastics from WWTPs were still released into the environmental waters due to the huge daily capacity. Sewage source determined the concentration, morphology feature, and chemical composition of microplastics to a certain extent. Compared with industrial wastewater, domestic wastewater possibly contained smaller microplastics of polyethylene and polypropylene with lower abundance. Furthermore, additional attention was provided on the flocculation process, drainage system, and treatment efficiency of microplastics with different shapes. This work is expected to provide some technical supports to guide the operation and management of WWTPs.


Assuntos
Poluentes Químicos da Água , Purificação da Água , China , Cidades , Monitoramento Ambiental , Microplásticos , Plásticos , Espectroscopia de Infravermelho com Transformada de Fourier , Eliminação de Resíduos Líquidos , Águas Residuárias/análise , Poluentes Químicos da Água/análise
18.
Poult Sci ; 100(1): 296-301, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33357693

RESUMO

There is a critical need for a rapid and simple method of qualitative and quantitative analysis of testosterone propionate (TP) and nandrolone (NT) residues in duck meat. In this study, we applied surface-enhanced Raman spectroscopy (SERS) coupled multivariate analysis for the classification and detection of TP and NT residues in duck meat. A total of 294 duck meat extract samples were obtained from duck breast meats based on a LC-MS/MS sample preparation method with slight modification including 102 duck meat extract samples without TP and NT, 43 duck meat samples containing TP, 47 duck meat extract samples containing NT, and 102 duck meat extract samples containing TP and NT. Raw Raman spectra were pretreated by using adaptive iteratively reweighted penalized least squares (airPLS), normalization and first derivative, and then the score values of first 10 principal components were selected as the inputs of the developed models. A particle swarm optimization-support vector classification (PSO-SVC) model was created to classify all the duck meat samples into the 4 groups (i.e., control group, TP group, NT group, and TP combined with NT group) with the classification accuracies of 99.49 and 100% for training set and test set, respectively. Furthermore, 2 least squares support vector regression (LS-SVR) models were developed to predict the TP values in samples with a determination coefficient (R2) value of 0.9316, root mean square error (RMSE) value of 2.1739, and ratio of prediction to deviation (RPD) value of 3.2189 for the test set, and NT values in samples with an R2 value of 0.9038, RMSE value of 2.2914, and RPD value of 2.9701 for the test set. Surface-enhanced Raman spectroscopy technology, in combination with multivariate analysis, has the potential to become the qualitative and quantitative analysis tool for TP and NT residues in duck meat extract.


Assuntos
Patos , Tecnologia de Alimentos , Carne , Nandrolona , Propionato de Testosterona , Animais , Cromatografia Líquida/veterinária , Tecnologia de Alimentos/métodos , Análise dos Mínimos Quadrados , Carne/análise , Análise Multivariada , Nandrolona/análise , Nandrolona/classificação , Análise Espectral Raman , Propionato de Testosterona/análise , Propionato de Testosterona/classificação
19.
Anal Chim Acta ; 1140: 18-29, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33218480

RESUMO

BACKGROUND: Metabolomics strategy was perform to identify the novel serum biomarkers linked to schizophrenia with the assistance of transcriptomics analysis. METHODS: Two analytical platforms, UPLC-Q-TOF MS/MS and 1H NMR, were used to acquire the serum fingerprinting profiles from a total of 112 participants (57 healthy controls and 55 schizophrenia patients). The differential metabolites were primarily selected after statistical analyses. Meanwhile, GSE17612 dataset downloaded from GEO database was implemented WGCNA analysis to discover crucial genes and corresponding biological processes. Based on metabolomics analysis, the metabolic distinctions were explored under the aid of transcriptomics. Then using Boruta algorithm identified the biomarkers, and LASSO regression analysis and Random Forest algorithm were used to evaluate the performance of the diagnostic model constructed by biomarkers selected. RESULTS: A total of four metabolites (α-CEHC, neuraminic acid, glyceraldehyde and asparagine) were selected as the biomarkers to establish diagnosis model. The performance of this model showed a higher accuracy rate to distinguish schizophrenia patients from healthy controls (area under the receive operating characteristic curve, 0.992; precision recall curve, 1.000, the mean accuracy of random forest algorithm, 95.00%). CONCLUSIONS: A four-biomarker model (α-CEHC, neuraminic acid, glyceraldehyde and asparagine) seems to be a good model for diagnosing schizophrenia patients. It might be helpful to guide the future studies on permitting early intervention designed to prevent disease progression.


Assuntos
Esquizofrenia , Espectrometria de Massas em Tandem , Biomarcadores , Humanos , Metabolômica , Esquizofrenia/diagnóstico , Esquizofrenia/genética , Transcriptoma
20.
Clin Interv Aging ; 15: 1971-1978, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33116451

RESUMO

Purpose: Vitamin D (25[OH]D) status and bone turnover markers (BTMs) are considered important determinants of bone quality, which is associated with the risk of hip fractures, including both femoral neck and intertrochanteric fractures, in older adults; however, the exact relationship of 25(OH)D and BTMs with the type and severity of hip fractures remains unclear and the present study aimed to identify any specific associations. Patients and Methods: According to the inclusion and exclusion criteria, 441 older female patients with hip fractures from 2015 to 2020 and 215 women without hip fractures were included in this cross-sectional study. According to Garden and AO/OTA classifications for femoral neck and intertrochanteric fractures, patients were divided into less severe (Garden I and Garden II; 31A1) and more severe (Garden III and Garden IV; 31A2 and 31A3) fracture groups. Levels of the serum osteoblast indicator, N-terminal/mid region (N-MID); the osteoclast indicator, beta-carboxy terminal telopeptide (ß-CTX); and 25(OH)D were analyzed. Results: For patients with less severe fractures, mean 25(OH)D levels were significantly higher than those with more severe fractures (17.67 vs 15.30 ng/mL, p = 0.006). Higher 25(OH)D levels were also observed in patients with less severe intertrochanteric fractures (p = 0.01). After adjustments for confounders, 25(OH)D remained a risk factor for patients with more severe fractures (p = 0.01), particularly those with intertrochanteric fractures (p = 0.011). No significant differences in BTMS were found between patients with less severe and more severe fractures. Conclusion: Levels of 25(OH)D were significantly associated with the severity of intertrochanteric, but not femoral neck, fractures. Neither 25(OH)D nor BTMs were associated with the type of hip fracture in older women. Separate consideration of intertrochanteric and femoral neck fractures may be appropriate when investigating the clinical association between 25(OH)D and the severity of hip fractures in older women.


Assuntos
Densidade Óssea/fisiologia , Fraturas do Quadril/sangue , Deficiência de Vitamina D/sangue , Vitamina D/análogos & derivados , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Remodelação Óssea , Estudos Transversais , Feminino , Fraturas do Colo Femoral/sangue , Fraturas do Quadril/complicações , Humanos , Vitamina D/sangue
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