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1.
Dalton Trans ; 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31595903

RESUMO

Two unprecedented organic-inorganic hybrid 1-D double chain germanomolybdates containing hepta-nuclear Cu-RE-pic heterometallic clusters [NH4]2[RE(H2O)5]2[Cu(pic)2]2[Cu(pic)2(H2O)2]3[α-GeMo12O40]2·22H2O [RE = La3+ (1), Ce3+ (2), and Hpic = 2-picolinic acid] were successfully obtained by the stepwise self-assembly strategy via the conventional solution method. The most striking structural feature of 1 and 2 is that the two plenary Keggin [α-GeMo12O40]4- polyoxoanions are joined by an organic-inorganic hybrid hepta-nuclear Cu-RE-pic {[RE(H2O)5]2[Cu(pic)2(H2O)2]3[Cu(pic)2]2}6+ heterometallic moiety. What is more interesting is that their adjacent structural units are connected together by {Cu(pic)2} bridges, forming a 1-D extended double chain architecture. Furthermore, the adsorption capacity of 1 toward dyes in aqueous solutions was deeply investigated. It is fascinating that 1 shows a good adsorption capacity toward basic violet 3 (BV 3) in aqueous solutions and the adsorption kinetics conforms to the second-order kinetic model.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31418680

RESUMO

A novel actinobacterium, designated strain NEAU-THZ27T, was isolated from soil collected from the Cornel peak in Jiaozuo, Henan Province, PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genusKribbella. The results of 16S rRNA gene sequence analysis showed that strain NEAU-THZ27T belongs to the genus Kribbella and was most closely related to Kribbella podocarpi YPL1T (98.96 %), Kribbella karoonensis Q41T (98.89 %), Kribbella aluminosa HKI 0478T (98.86%) and Kribbella hippodromi S1.4T (98.85 %), similarities to other type strains of species of the genus Kribbella were found to be less than 98.7 %. Phylogenetic analyses using the 16S rRNA gene sequence and multilocus sequence analysis using the concatenated gene sequences of the gyrB, rpoB, recA, relA and atpD genes all showed that the strain formed a separate branch in the genus Kribbella. The cell wall contained ll-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates were ribose and glucose. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H4). Major fatty acids were iso-C16 : 0, iso-C14 : 0 and anteiso-C15 : 0, these chemotaxonomic data supported the affiliation of strain NEAU-THZ27T to the genus Kribbella. The DNA G+C content was 68.0 mol%. Furthermore, the strain could be clearly distinguished by concatenated gene genetic distances, the combination of DNA-DNA hybridization results and some phenotypic characteristics. Therefore, it is proposed that strain NEAU-THZ27T represents a novel species of the genus Kribbella, for which the name Kribbella jiaozuonensis sp. nov. is proposed. The type strain is NEAU-THZ27T (=CGMCC 4.7504T=DSM 105535T).

3.
Inorg Chem ; 58(17): 11636-11648, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31415160

RESUMO

Four novel rare-earth (RE) and Sb-oxo clusters simultaneously bridging antimonotungstates (ATs) [H2N(CH3)2]8Na6H8{[RE4(H2O)6Sb6O4](SbW10O37)2(SbW8O31)2}·24H2O [RE = Dy3+ (1), Er3+ (2), Y3+ (3), Ho3+ (4)] were triumphantly isolated by using Na9[B-α-SbW9O33]·19.5H2O, dimethylamine hydrochloride, SbCl3, and RE(NO3)3·6H2O in acidic aqueous solution. Their tetrameric polyoxoanions are all established by two [SbW10O37]11- and two [B-α-SbW8O31]11- segments encapsulating a RE-Sb heterometal [RE4(H2O)6Sb6O4]22+ cluster. Under the O→W LMCT excitation at 268 nm of AT segments, RE ions in 1 and 2 can absorb the 3T1u→1A1g emission energy of AT segments, giving rise to the sensitization of AT segments to the emission of RE ions. Through the sensitization of AT segments, the emission intensities of RE ions in 1 and 2 are increased and lifetimes are prolonged. Photoluminescence and energy transfer measurements indicate that Dy3+ ions in 1 can obtain more energy from AT segments than to Er3+ ions in 2. Furthermore, the sensitization effects of AT segments to different f-f transitions of RE ions have been compared in 1 and 2. Under the sensitization of AT segments, the 4F9/2→6H15/2 transition obtains the most energy compared with the 4F9/2→6H13/2 and 4F9/2→6H11/2 transitions in 1, and the 2H11/2→4I15/2 transition obtains more energy than the 4S3/2→4I15/2 transition in 2. It seems that the better energy level match of 3T1u→1A1g transition of AT segments with f-f emission transitions of RE ions will lead to a stronger sensitization effect.

4.
BMC Plant Biol ; 19(1): 298, 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31286900

RESUMO

BACKGROUND: Homeodomain-leucine zipper (HD-ZIP) transcription factors play important roles in the growth, development and stress responses of plants, including (presumably) physic nut (Jatropha curcas), which has high drought and salinity tolerance. However, although physic nut's genome has been released, there is little knowledge of the functions, expression profiles and evolutionary histories of the species' HD-ZIP genes. RESULTS: In this study, 32 HD-ZIP genes were identified in the physic nut genome (JcHDZs) and divided into four groups (I-IV) based on phylogenetic analysis with homologs from rice, maize and Arabidopsis. The analysis also showed that most of the JcHDZ genes were closer to members from Arabidopsis than to members from rice and maize. Of the 32 JcHDZ genes, most showed differential expression patterns among four tissues (root, stem cortex, leaf, and seed). Expression profile analysis based on RNA-seq data indicated that 15 of the JcHDZ genes respond to at least one abiotic stressor (drought and/or salinity) in leaves at least at one time point. Transient expression of a JcHDZ16-YFP fusion protein in Arabidopsis protoplasts cells showed that JcHDZ16 is localized in the nucleus. In addition, rice seedlings transgenically expressing JcHDZ16 had lower proline contents and activities of antioxidant enzymes (catalase and superoxide dismutase) together with higher relative electrolyte leakage and malondialdehyde contents under salt stress conditions (indicating higher sensitivity) than wild-type plants. The transgenic seedlings also showed increased sensitivity to exogenous ABA, and increases in the transcriptional abundance of several salt stress-responsive genes were impaired in their responses to salt stress. Further data on JcHDZ16-overexpressing plants subjected to salt stress treatment verified the putative role of JcHDZ genes in salt stress responses. CONCLUSION: Our results may provide foundations for further investigation of functions of JcHDZ genes in responses to abiotic stress, and promote application of JcHDZ genes in physic nut breeding.


Assuntos
Jatropha/genética , Oryza/genética , Proteínas de Plantas/genética , Fatores de Transcrição/genética , Estudo de Associação Genômica Ampla , Jatropha/metabolismo , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Fatores de Transcrição/metabolismo , Transcriptoma
5.
Inorg Chem ; 58(15): 9706-9712, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31318540

RESUMO

A family of inorganic-organic hybrid one-dimensional (1-D) chain iron-lanthanide (Ln) heterometal encapsulated tungstotellurates [H2N(CH3)2]8K2Na4[Ln2(Ac)2(H2O)4Fe2(Hpdca)2(B-ß-TeW9O33)2][Ln2(H2O)8Fe2(Hpdca)2(B-ß-TeW9O33)2]·50H2O [Ln = Eu3+ (1), Tb3+ (2), Dy3+ (3), Er3+ (4), HAc = acetate acid, H2pdca = 2,5-pyridinedicarboxylic acid] were prepared using a facile "one-pot" reaction. The molecular structures of 1-4 consist of an intriguing organic-ligand-connecting Fe-Ln heterometal inserted tetrameric unit [Ln2(Ac)2(H2O)4Fe2(Hpdca)2(B-ß-TeW9O33)2][Ln2(H2O)8Fe2(Hpdca)2(B-ß-TeW9O33)2]14-. Appealingly, the tetrameric unit is composed of two sandwich-type subunits [Ln2(Ac)2(H2O)4Fe2(Hpdca)2(B-ß-TeW9O33)2]8- and [Ln2(H2O)8Fe2(Hpdca)2(B-ß-TeW9O33)2]6-, in which each sandwich-type subunit can be regarded as a derivative of two Ln-organic complexes substituting two external FeIII ions in the classic Krebs-type [Fe4(H2O)10(ß-TeW9O33)2]4- fragment. Furthermore, adjacent tetrameric units are interconnected into a 1-D chain arrangement by Hpdca- bridges. 1-4 represent the first examples of Fe-Ln heterometal encapsulated tungstotellurates. Comprehensive magnetic measurements of 3 imply the possible single-molecule magnet properties in 3 with an estimated relaxation time τ0 ≈ 6.06 × 10-6 s at Hdc = 0 Oe and τ0 ≈ 6.97 × 10-5 s at Hdc = 1500 Oe. In addition, the solid-state photoluminescence spectra of 1 and 2 at room temperature exhibit the typical f-f transitions of Ln cations. The Commission International d'Eclairage (CIE) color coordinates of (0.540 24, 0.442 44) for 1 and (0.428 71, 0.428 30) for 2 along with the correlated color temperatures of 1995 and 3278 K, dominant wavelengths of 586 and 578 nm, and color purities of 95.09% and 57.27% for 1 and 2 are obtained.

6.
Int J Syst Evol Microbiol ; 69(10): 3268-3275, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31355738

RESUMO

A Gram-stain-positive, aerobic, heterotrophic, non-spore-forming and rod-shaped strain, designated SJ-23T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Langfang, Hebei Province, central PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Agromyces. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipid and three unidentified lipids. The predominant menaquinones detected were MK-12, MK-11 and MK-10. Major fatty acids were identified as anteiso-C17 : 0, anteiso-C15 : 0 and iso-C16 : 0. The 16S rRNA gene sequence analysis showed that strain SJ-23T belongs to the genus Agromyces with high sequence similarities to Agromyces ramosus DSM 43045T (99.2 %), Agromycescerinussubsp. cerinus DSM 8595T (98.8 %) and Agromyces cerinussubsp. nitratus DSM 8596T (98.6 %). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a separate branch in the genus Agromyces. Furthermore, the combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain SJ-23T could be distinguished from its closest relatives. Therefore, it is proposed that strain SJ-23T represents a novel species of the genus Agromyces, for which the name Agromycestardus sp. nov. is proposed. The type strain is SJ-23T (=CGMCC 4.7419T=DSM 105049T).


Assuntos
Actinobacteria/classificação , Filogenia , Rizosfera , Microbiologia do Solo , Triticum/microbiologia , Actinobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/química
7.
Int J Syst Evol Microbiol ; 69(9): 2914-2920, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31287395

RESUMO

One novel actinobacterial isolate, designated strain NEAU-G17T, was isolated from muddy soil collected from a riverbank in Chad and characterised using polyphasic approach. Morphological and chemotaxonomic characteristics of this strain coincided with those of the genus Actinomadura. The 16S rRNA gene sequence analysis showed that strain NEAU-G17T was most closely related to Actinomadura oligospora JCM 10648T (98.7 %) and Actinomadura gamaensis NEAU-Gz5T (98.6 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G17T formed a cluster with Actinomadura oligospora JCM 10648T, Actinomadura gamaensis NEAU-Gz5T and Actinomadura rupiterrae CS5-AC15T (96.4 %). However, the combination of DNA-DNA hybridisation values and some phenotypic characteristics allowed the isolate to be differentiated from their most closely related species. The major menaquinones were identified as MK-9(H4), MK-9(H6) and MK-9(H8). Their polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as C16 : 0, iso-C16 : 0 and C18 : 1 ω9c. Therefore, it is proposed that strain NEAU-G17T should be classified as representative of a novel species of the genus Actinomadura, for which the name Actinomadura logoneensis sp. nov. is proposed. The type strains is NEAU-G17T (=CGMCC 4.7411T=DSM 105122T).


Assuntos
Actinobacteria/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , Chade , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
8.
Int J Syst Evol Microbiol ; 69(10): 3093-3099, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31329533

RESUMO

A novel bacterial strain, designated NEAU-SA2T, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach. The cells were aerobic, Gram-stain-positive, non-flagellated and rod-coccus-shaped. The strain grew optimally at 28 °C, pH 7.0 and with 0 % NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271T (98.41 %), which was further confirmed by multilocus sequence analysis. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0; MK-9(H2) was the predominant respiratory quinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The peptidoglycan type was A3α, and the cell-wall sugars were glucose and galactose. The genomic G+C content of strain NEAU-SA2T was 67.04 mol%. The average nucleotide identity values between NEAU-SA2T and A. cupressi DSM 24664T and A. silvisoli CCTCC AB 2017271T were 88.57-90.94 %. The digital DNA-DNA hybridisation values between strain NEAU-SA2T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa. Therefore, strain NEAU-SA2T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter celericrescens sp. nov. is proposed. The type strain is NEAU-SA2T (=DSM 106718T=CCTCC AB 2017272T).


Assuntos
Arthrobacter/classificação , Florestas , Filogenia , Microbiologia do Solo , Arthrobacter/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Parede Celular/química , China , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Hibridização de Ácido Nucleico , Peptidoglicano/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
9.
Artigo em Inglês | MEDLINE | ID: mdl-31222464

RESUMO

A Gram-stain positive, aerobic, rod-shaped bacterium, designated strain NEAU-85T, was isolated from rhizosphere soil of lettuce and characterised using a polyphasic approach. Strain NEAU-85T was found to be catalase positive, motile and able to grow at between 10 and 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-85T belongs to the genus Cryobacterium and is closely related to the type strain Cryobacterium psychrotolerans JCM 13925T (98.5%) and also to Leifsonia kafniensis NCCB 100216T (97.6%). Multilocus sequence analysis using the concatenated gene sequences of the atpD, dnaK, recA, rpoB, sevY and ychF genes showed that strain NEAU-85T belongs to the genus Cryobacterium. The digital DNA-DNA hybridization values revealed that strain NEAU-85T is distinct from its close phylogenetic neighbour C. psychrotolerans. The menaquinones were identified as MK-10 and MK-11. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phospholipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as anteiso-C15:0, anteiso-C15:1 and iso-C16:0. The genomic DNA G + C content of strain NEAU-85T was determined to be 68.9 mol%. The DNA-DNA hybridization value between them was less than 70%. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-85T can be concluded to represent a novel species of the genus Cryobacterium, for which the name Cryobacterium tepidiphilum sp. nov. is proposed. The type strain is NEAU-85T (= CCTCC AA 2018035T = JCM 32545T).

10.
Inorg Chem ; 58(13): 8442-8450, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31247874

RESUMO

Two multi-Ce3+-substituted selenotungstates (STs), [HDMEA][H2N(CH3)2]4H3Na4[Ce2(H2O)6(DMEA)W4O9(α-SeW9O33)3]·26H2O (1) and [H2N(CH3)2]10H4Na10[Ce2W4O9(H2O)7(α-SeW9O33)3]2·63H2O (2), were prepared by the one-pot approach of sodium tungstate, sodium selenite, and lanthanide nitrate in an acidic water solution in the presence of N, N-dimethylethanolamine (DMEA) or dimethylamine hydrochloride (DMAHC). 1 was obained in the presence of DMEA, whereas 2 was synthesized in the presence of DMAHC. The trimeric polyoxoanion of 1 contains an unusual V-shaped [Se3W29O103]20- group embracing a prominent heterometal oxide fragment, [Ce2(H2O)6(DMEA)W2O5]8+, and the hexameric polyoxoanion of 2 is constructed from two equivalent trimeric [Ce2W4O9(H2O)7(SeW9O33)3]224- subunits through two -O-W-O-Ce-O- linkages. The most worthy of attention is that the polyoxoanion of 1 can be approximatively viewed as a half of the polyoxoanion of 2 because of the coordination and blocking effect of DMEA. The stability of 1 and 2 in different water pH values was studied by electrospray ionization mass spectroscopy (ESI-MS), and the results manifest that 1 and 2 are stable in pH = 3.5-7.5 and 3.5-7.0, respectively. The oxidation reactions of aromatic sulfides catalyzed by H2O2 were studied when 1 or 2 worked as a catalyst, and experimental results reveal that 1 or 2 can serve as available catalysts for the oxidation of aromatic sulfides under mild conditions.

11.
Int J Syst Evol Microbiol ; 69(9): 2703-2708, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31210626

RESUMO

A Gram-stain-positive, aerobic actinomycete strain, designated NEAU-LLET, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, north-east China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLET belonged to the genus Microbacterium, with highest sequence similarity to Microbacterium pseudoresistens DSM 22185T (98.2 %). The predominant menaquinones were MK-13 and MK-14. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid. The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. However, DNA-DNA relatedness and physiological and biochemical data showed that strain NEAU-LLET could be distinguished from its closest relative. Therefore, strain NEAU-LLET represents a novel species of the genus Microbacterium, for which the name Microbacteriumbovistercoris sp. nov is proposed, with NEAU-LLET (=CCTCC AA 2018025T=JCM 32662T) as the type strain.


Assuntos
Actinobacteria/classificação , Bovinos/microbiologia , Esterco/microbiologia , Filogenia , Actinobacteria/isolamento & purificação , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Feminino , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/química
12.
Inorg Chem ; 58(10): 7078-7090, 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-31066283

RESUMO

Two penta-REIII encapsulated tetravacant Dawson selenotungstates [H2N(CH3)2]10H3[SeO4RE5(H2O)7(Se2W14O52)2]·40H2O (RE = Dy3+ (1), Gd3+ (2)) were prepared by a one-step assembly strategy under bench conditions. Notably, the penta-REIII-substituted [SeO4RE5(H2O)7(Se2W14O52)2]12- polyoxoanion consists of two equivalent tetravacant Dawson [Se2W14O52]12- fragments linked by a central penta-REIII {SeO4RE5(H2O)7} cluster through 16 lacunary oxygen atoms and four bridging oxygen atoms from two [Se2W14O52]12- fragments. Furthermore, a series of cetyltrimethylammonium bromide (CTABr) encapsulated 1@CTA nanomaterials were prepared by the microwave method by controlling different reaction times and temperatures. The effects of time and temperature on the morphologies of 1@CTA nanomaterials were characterized by SEM images, and 1@CTA-5min prepared at 80 °C has a relatively uniform size of about 190 nm. Moreover, the photoluminescence properties of 1 and 1@CTA nanomaterials were investigated systematically, indicating that 1@CTA-5min prepared at 80 °C exhibits the strongest emission due to its smallest size and high dispersion. The energy transfer from the ST fragments to Dy3+ ions in 1 and 1@CTA-5min were proved by time-resolved emission spectra (TRES) and the change in CIE coordinates with increasing time, and their energy transfer mechanism diagram was demonstrated.

13.
Toxicol Mech Methods ; 29(7): 499-510, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31050318

RESUMO

The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is classified as a Group 1 human carcinogen. It is metabolically activated by P450 enzymes to intermediate methylate and pyridyloxobutylate DNA, resulting in the formation of DNA adduct that is critical for the carcinogenicity of NNK. To directly and objectively examine the DNA adduct formation profiles without the complexity of factors in vivo, in the present study, five kinds of methyl DNA adducts were first identified in the incubation model of NNK established with human lung epithelial cells (BEAS-2B). The level of methyl DNA adducts and metabolites of NNK were quantitatively analyzed, respectively. With the increase of exposure time and dose, the level of methyl DNA adducts and metabolites increased. Furthermore, with the changes of the activity of P450 enzymes, which is the main enzyme regulating the α-hydroxylation of NNK, we found the levels of both methyl adducts and metabolites formed via α-hydroxylation in experimental groups showed the same trend compared with those in control group, while the metabolites formed via other pathways changed in the opposite trend. The result proves that the methyl adducts induced by NNK generate via α-hydroxylation pathway in BEAS-2B cells.

14.
Inorg Chem ; 58(11): 7229-7235, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-30994335

RESUMO

The development of logical visible-light-driven heterogeneous photosystems for water splitting is a subject of new research. As the first example of a noble-metal-free photocatalyst for both H2 and O2 production, a high-nuclear {CuI24(µ3-Cl)8(µ4-Cl)6}-based polyoxometalate (POM)@metal-organic framework (MOF) (ZZULI-1) is rationally designed to serve as a robust dual-functionalized photocatalyst. ZZULI-1 exhibits highly efficient photocatalytic H2 evolution (6614 µmol g-1 h-1) and O2 evolution (1032 µmol g-1 calculated for the first 6 min). The {CuI24(µ3-Cl)8(µ4-Cl)6} clusters and mixed POMs not only work as the active units for H2 and O2 production, respectively, but also improve the effective electron transfer between the photosensitizer and ZZULI-1. The highly stable dual-functionalized ZZULI-1 affords new penetrations into the development of cost-effective high-nuclear cluster-based POM@MOFs for efficient solar-to-fuel generation.

15.
J Org Chem ; 84(9): 4992-5004, 2019 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-30957502

RESUMO

Peptidomimetics containing ( S)- or ( R)-imidazolidin-2-one-4-carboxylate (Imi) have been obtained by the expedient in-peptide cyclization of ( S)- or ( R)-α,ß-diaminopropionic acid (Dap) residues. These Imi scaffolds behave as proline analogues characterized by a flat structure and a trans-restricted geometry of the preceding peptide bond and induce well-defined secondary structures in a biomimetic environment. While ( S)-Imi peptides adopted a γ'-turn conformation, ( R)-Imi induced the contemporary formation of a γ-turn and a rare 11-membered H-bonded structure in the 2→4 opposite direction of the sequence, identified as a ε-turn. In order to exploit these Imi scaffolds as general promoters of unusual secondary structures, proteinaceous side chains have been introduced at the N1 position of the five-membered ring, potentially mimicking any residues. Finally, the Imi rings have been equipped with unnatural side chains or with functionalized substituents, which can be utilized as linkers to chemoselectively bind the Imi-peptides onto nanoparticles, biomaterials, or diagnostic probes.

16.
Inorg Chem ; 58(7): 4657-4664, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30882216

RESUMO

On the basis of the synergistic strategy of lacunary polyoxometalate structure-directing function and chiral ligand inducting role, two pairs of enantiomeric polyoxotungstates, (NH4)4(TMA)4[Zr4(µ3-O)2(l-/d-mal)2(B-α-HSiW10O37)2] (TMA = tetramethylammonium, mal = malate (C4H5O5); l-mal for 1a, d-mal for 1b) and (NH4)4(TMA)4[Zr4(µ3-O)2(l-/d-mal)2(B-α-PW10O37)2] (l-mal for 2a, d-mal for 2b), and a mesomeric polyoxotungstate, (NH4)3Na2K5[Zr4(µ3-O)2(l-mal)(d-mal)(B-α-SiW10O37)2] (3), were hydrothermally synthesized. 1a, 2a and 1b, 2b respectively exhibit 1-D 21 right- and left-hand helical chains formed by hydrogen-bonding interactions, and 3 forms a 3-D (3,10)-connected framework by Na+/K+ ions with {418.624.83}{43}2 topology. These homochiral compounds represent the first examples of enantiomerically pure ZrIV-substituted Keggin POMs. In this system, {Zr4(µ3-O)2(l-/d-mal)2} clusters transfer chirality from d- or l-mal to Keggin polyoxotungstate dimeric clusters, which was demonstrated by structural comparison between the homochiral architecture and mesomer as well as circular dichroism spectra of enantiomers. UV-vis diffuse reflectance spectra reveal that 1-3 are potential semiconductor materials. In addition, 1 and 2 exhibit second harmonic generation response with their response intensities of 0.8 times that of KDP.

17.
Dalton Trans ; 48(11): 3730-3742, 2019 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-30806427

RESUMO

Two kinds of organic-inorganic 3d-4f heterometal hybrids based on plenary α-Keggin-type germanotungstates [Cu2(H2O)3(PA)3] [Ln0.5Na0.5Cu2(H2O)12(PA)3][α-GeW12O40]·5H2O [Ln = La3+ (1), Ce3+ (2)] and [Cu2(H2O)2(PA)3][Cu(PA)2][Ln(H2O)7][α-GeW12O40]·7H2O [Ln = Tb3+ (3), Dy3+ (4), HPA = 2-picolinic acid] were prepared via the strategy of combining an in situ assembly reaction and stepwise synthesis in the aqueous solution. The most remarkable structural characteristic of 1-2 is that neighboring structural units are connected into a 1-D chain alignment by the bridging di-copper [Cu2(H2O)3(PA)3]+ subunits, whereas the most outstanding structural feature of 3-4 is that neighboring structural units are interconnected to generate a zigzag 1-D chain alignment by the bimetallic bridging [Cu2(H2O)3(PA)3]+ subunits, and then adjacent zigzag 1-D chains are integrated into a fascinating 2-D sheet structure by heterobimetallic bridging {Tb(H2O)7[Cu(PA)2]0.5}3+ subunits and [Cu(PA)2] groups. As far as we know, 1-4 represent the first examples of plenary Keggin heterometal germanotungstates including organic 3d-4f heterometal subunits so far. The electrochemical sensing properties towards the detection of Acetaminophen of 1/3@CMWCNT-Nafion/GCE electrochemical sensors were investigated, showing that 1/3@CMWCNT-Nafion/GCE electrochemical sensors exhibit good stability and good sensing performance towards AC detection.

18.
Inorg Chem ; 58(5): 3479-3491, 2019 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-30762353

RESUMO

Three kinds of distinguishing ala-decorated RE-incorporated (ala = l-alanine, RE = rare-earth) polytungstoarsenate hybrids containing {As2W19O68} building blocks [H2N(CH3)2]13Na3H2[Eu4W5(H2O)10(ala)3O14(As2W19O68)2]·35H2O (1), [H2N(CH3)2]12Na2H2[RE4W6(H2O)8(ala)4O15(OH)2(As2W19O68)2]·36H2O [RE = Gd3+ (2), Tb3+ (3)], and [H2N(CH3)2]12Na4[RE4W6(H2O)10(ala)2O15(OH)2(As2W19O68)2]·40H2O [RE = Dy3+ (4), Ho3+ (5), Er3+ (6), Yb3+ (7), Lu3+ (8)] were made in a facile one-step assembly strategy. The polyoxoanion skeletons of 1-8 are very similar, and all can be described as two [As2W19O68]16- moieties encapsulating an ala-decorated W-O-RE heterometallic cluster ([Eu4W5(H2O)10(ala)3O14]14+ for 1, [RE4W6(H2O)8(ala)4O15(OH)2]16+ for 2-3, and [RE4W6(H2O)10(ala)2O15(OH)2]16+ for 4-8, resulting in a four-leaf--clover-shaped tetrameric structure; however, the major discrepancy in the ala-decorated W-O-RE heterometallic clusters lies in the number of ala molecules equaling to three, four, and two, which may result from the various coordination geometries of RE ions and the different construction modes of W-O-RE heterometallic clusters. It should be emphasized that the carboxyl groups of ala ligands only coordinate to the W centers in 4-8, while they not only bridge the W centers together but also combine RE ions together in 1-3. Besides, the solid luminescent properties and lifetime decay behaviors of 1, 3, 4, 5, and 6 were systematically investigated at ambient temperature, and time-resolved fluorescence spectra of 1, 3, and 5 were also examined. Furthermore, the electrochemical response properties of composite CS-CMWCNs-1, 3, or 5/GCE electrochemical sensors toward the dopamine detection were investigated, which will offer the possibility of RE-incorporated polyoxometalates for acting as electrochemical response electrode materials.

19.
Int J Syst Evol Microbiol ; 69(4): 1001-1008, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30702420

RESUMO

A novel actinomycete, designated strain NEAU-TCZ24T, was isolated from soil and characterized using a polyphasic approach. The results of phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Cellulomonas and formed a stable clade with its closest relatives Cellulomonas terrae JCM 14899T (98.4 % 16S rRNA gene sequence similarity), Cellulomonas xylanilytica JCM 14281T (97.9 %) and Cellulomonas humilata JCM 11945T (97.7 %). The major menaquinones were identified as MK-9(H4) and MK-8(H4). The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositolmannoside, a ninhydrin-positiveglycolipid, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C15 : 0, C18 : 1ω9c, C16 : 0 and anteiso-C17 : 0. Moreover, morphological and chemotaxonomic properties of NEAU-TCZ24T also confirmed the affiliation of the isolate to the genus Cellulomonas. However, multilocus sequence analysis based on five other house-keeping genes (gyrB, rpoB, recA, relA and atpD), DNA-DNA relatedness, physiological and biochemical data indicated that NEAU-TCZ24T could be distinguished from its closest relatives. Therefore, it is proposed that NEAU-TCZ24T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas rhizosphaerae sp. nov. is proposed. The type strain is NEAU-TCZ24T (=CCTCC AA 2018042T=JCM 32383T).


Assuntos
Cellulomonas/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , Cellulomonas/isolamento & purificação , China , DNA Bacteriano/genética , Ácidos Graxos/química , Genes Bacterianos , Tipagem de Sequências Multilocus , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/química
20.
Int J Syst Evol Microbiol ; 69(5): 1320-1326, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30801239

RESUMO

A novel actinomycete, designated strain NEAU-YY265T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07T and Jiangella alkaliphila D8-87T. The chemotaxonomic properties of strain NEAU-YY265T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones. The novel strain produced branching mycelium which fragmented into short or elongated rods. The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0. The DNA G+C content was 72.6 mol%. Strain NEAU-YY265T was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results. Thus, strain NEAU-YY265T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp. nov. is proposed. The type strain is NEAU-YY265T (=JCM 32551T=CGMCC 4.7475T).


Assuntos
Actinobacteria/classificação , Filogenia , Rizosfera , Microbiologia do Solo , Triticum/microbiologia , Actinobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Parede Celular/química , China , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Peptidoglicano/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
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