Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 248
Filtrar
1.
Inorg Chem ; 59(20): 15355-15364, 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33001637

RESUMO

A novel organic-inorganic hybrid cerium-encapsulated selenotungstate comprising three polyoxotungstate building units Na16H6{[Ce3W4O10(H2O)9(CH3COO)3]2(Se2W7O30)(B-α-SeW9O33)4}·(C5H8NBO3)·119H2O (1) was synthesized by the portfolio approach of an in situ self-assembly reaction and step-by-step synthesis. The hybrid polyoxoanion of 1 is constructed from an acetate-coordinated heterometallic {[(Ce3W4O10)(H2O)9(CH3COO)3]2(Se2W7O30)}10+ core encompassing four trilacunary [B-α-SeW9O33]8- subunits. Interestingly, the heterometallic core contains a remarkable [Se2W7O30]10- building block in which seven WVI atoms form a W7 plane and two SeIV atoms are situated on two opposite faces of the W7 plane. In addition, the electrochemical performances of the 1@CFMCN composite (CFMCN: carboxyl-functionalized multiwalled carbon nanotube) were investigated. The 1@CFMCN/GCE (GCE: glass carbon electrode) sensor demonstrates a promising potential in electrochemically sensing dopamine (DPA) or paracetamol (PCM) or even simultaneously detecting DPA and PCM with low limits of 0.053 µM for DPA and 2.03 µM for PCM over a wide linear range at high sensitivity.

2.
Shanghai Kou Qiang Yi Xue ; 29(4): 386-389, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-33089287

RESUMO

PURPOSE: The purpose of this study was to investigate the periodontal status in adult periodontal disease patients with malocclusion treated with digital clear aligners. METHODS: Thirty-three patients with periodontal disease who needed orthodontic treatment were selected. The patients were randomly divided into 2 groups, digital clear aligners group (experimental group, 16 patients) and fixed appliances group (control group, 17 patients). Bleeding index (BI), probing depth(PD), plaque index(PLI) and gingival index(GI) were recorded at baseline and 1, 3, 6 and 9 months during orthodontic treatment. SPSS 17.0 software package was used to analyze and compare the data of periodontal status between two groups. RESULTS: 1, 3, 6, and 9 months after orthodontic treatment, clinical parameters of the control group were significantly higher than baseline(P<0.05). The same measurements of the experimental group showed no significant differences at 1, 3, 6, and 9 months of treatment (P>0.05). After 1, 3, 6, and 9 months of treatment, the clinical parameters of BI, PLI and GI in the experimental group were significantly lower than the control group(P<0.05); PD in the experimental group was smaller than the control group, but there was no significant difference(P>0.05). CONCLUSIONS: Compared with conventional fixed appliance, clear aligner of digitalization can more effectively maintain periodontal heath in adult periodontal disease patients with malocclusion.


Assuntos
Má Oclusão , Aparelhos Ortodônticos Removíveis , Doenças Periodontais , Adulto , Índice de Placa Dentária , Humanos , Má Oclusão/terapia , Doenças Periodontais/terapia , Índice Periodontal
3.
Inorg Chem ; 59(16): 11287-11297, 2020 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-32799459

RESUMO

Six organic-inorganic hybrid pyridine-4-carboxylate-decorated organotin (OT)-lanthanide (Ln) heterometallic antimotungstates [Ln(H2O)6(pca)]H[Sn(CH3)2(H2O)]3[B-ß-SbW9O33]·12H2O [Ln = La3+ (1), Ce3+ (2), Pr3+ (3), Nd3+ (4), Sm3+ (5), Eu3+ (6); Hpca = isonicotinic acid] have been prepared with the help of the structure-directing effect of the trivacant [B-α-SbW9O33]9- segment toward [(CH3)2Sn]2+ and Ln3+ ions in an acidic water medium. The prominent architecture characteristic is that their structural units consist of a trivacant [B-ß-SbW9O33]9- segment stabilized by three [Sn(CH3)2(H2O)]2+ groups and a [Ln(H2O)6(pca)]2+ cation, which are interconnected to propagate an intriguing two-dimensional (2D) network. For all we know, 1-6 stand for the first 2D OT-Ln heterometallic polyoxometalates. Furthermore, luminescence performances of solid-state 3-6 were deeply surveyed at ambient temperature. Energy migration from [B-ß-SbW9O33]9- and pca- to Sm3+ centers in 5 was also studied. Comparative studies demonstrate that the contribution of [B-ß-SbW9O33]9- sensitizing the emission of Sm3+ is prominently larger than that of pca- sensitizing the emission of Sm3+ in the emission process of 5. Most interestingly, 6 as a fluorescence probe exhibits high selectability and sensitivity for recognizing Zn2+ and Cu2+ in water.

4.
Dalton Trans ; 49(35): 12401-12410, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32852009

RESUMO

A trimeric tri-Tb3+-including antimonotungstate (AMT) hybrid Na17{(WO4)[Tb(H2O)(Ac)(B-α-SbW9O31(OH)2)]3}·50H2O (Tb3W28) was successfully synthesized, in which the capped tetrahedral {WO4} group plays a significant template role in directing the aggregation of three [B-α-SbW9O33]9- fragments and three Tb3+ ions. Eu3+/Tb3+/Dy3+/Gd3+-codoped AMT materials based on Tb3W28 were firstly prepared and their luminescence properties were investigated. The red emitter Eu3+, yellow emitter Dy3+, and nonluminous Gd3+ ions were codoped into Tb3W28 to substitute Tb3+ ions for investigating the energy transfer (ET) mechanism among Eu3+, Tb3+, and Dy3+ ions. Upon the 6H15/2 → 4I13/2 excitation at 389 nm of the Dy3+ ion, the ET1 mechanism (Dy3+ → Tb3+) was confirmed as a non-radiative dipole-dipole interaction. Under the 7F6 → 5L10 excitation at 370 nm of the Tb3+ ion, the ET2 mechanism (Tb3+ → Eu3+) was identified as a non-radiative quadrupole-quadrupole interaction. Under excitation at 389 nm, the two-step successive Dy3+ → Tb3+ → Eu3+ ET3 process was proved in Dy1.2Tb3zEu0.03Gd1.77-3zW28. Through changing the excitation wavelengths, the emission color of Dy1.2Tb1.2Eu0.03Gd0.57W28 can vary from blue to yellow, in which a near-white-light emission case was observed upon excitation at 378 nm. This work not only provides a systematic ET mechanism study of hetero-Ln-codoped AMTs, but also offers some useful guidance for designing novel performance-oriented Ln-codoped polyoxometalate-based materials.

5.
Arch Gynecol Obstet ; 302(6): 1529-1538, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32808113

RESUMO

PURPOSE: The primary aim of this study was to evaluate the diagnostic accuracy of colposcopy in identifying high-grade squamous intraepithelial lesion or worse (HSIL+) and the characteristic performance of colposcopic images with various severity levels of cervical lesions. METHODS: The medical records from 1828 women who underwent colposcopy at Affiliated Hospital of Tongji University from February 2016 to March 2019 were reviewed. Human papilloma virus (HPV) GenoArray test kit (HybriBio Ltd) and Thinprep cytologic test (TCT, Hologic, USA) were used to perform HPV genotyping and cytology. All colposcopic images were collected from the standard-of-care colposcope (Leisegang 3ML LED) and evaluated based on the 2011 International Federation of Cervical Pathology and Colposcopy (IFCPC) Colposcopy Standards. The linear by linear association, Pearson χ2 test, χ2 test, Kappa test, McNemar test and risk test were used to perform statistical analyses. RESULTS: The consistency between colposcopy and biopsy pathology was 59.35% with the moderate strength of kappa coefficient of 0.464. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of colposcopy and cytology for HSIL+ were 56.29%, 93.82%, 77.47%, 85.04% and 37.13%, 98.49%, 90.29%, 80.58%, respectively. The colposcopic features of HSIL+ were as follows: (1) thick or bulgy acetowhite epithelium with sharp border; (2) completely nonstained of Lugol's iodine; (3) type III/IV/V of gland openings; (4) punctation or atypical vessels. CONCLUSION: The data and findings herein provide the resource for evaluating the diagnostic value of colposcopy, and suggested that the accuracy of colposcopy is required to be further improved.

6.
Nanoscale ; 2020 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-32724947

RESUMO

Two unique nanosized Nd3+-substituted selenotungstates Na9K8{[W3Nd2(H2O)3(NO3)O6](B-α-SeW9O33)2(α-Se2W14O52)}·35H2O (1) and [H2N(CH3)2]7H9Na4{[W2Nd2(H2O)8O6(OH)2(ß-Se2W14O52)][W3Nd2(H2O)6O7(B-α-SeW9O33)2]2}·84H2O (2) were prepared by reacting NaSeO3, Na2WO4·2H2O with Nd(NO3)3·6H2O in aqueous solution by controlling different cations and pH values. 1 was synthesized at pH = 4.3 in the presence of KCl, whereas 2 was synthesized at pH = 3.0 in the presence of [H2N(CH3)2]·Cl. The most striking structural feature of 1 and 2 is the coexistence of vacant Keggin and Dawson segments in the polyoxoanion, which is extremely rare in the field of polyoxometalate chemistry. The trimeric polyoxoanion of 1 can be perceived as a fusion of one α-type tetravacant Dawson [α-Se2W14O52]14- unit and two trivacant Keggin [B-α-SeW9O33]8- segments sealing a trigonal bipyramid pentanuclear [W3Nd2(H2O)3(NO3)O6]11+ cluster, while the pentameric polyoxoanion of 2 can be described as one ß-type tetravacant Dawson [ß-Se2W14O52]14- fragment and four trivacant Keggin [B-α-SeW9O33]8- segments anchoring a saddle-shaped [W8Nd6(H2O)20O20(OH)2]24+ cluster. In addition, the measurements of catalytic oxidation of aromatic thioethers show that 2 as a catalyst possesses extremely outstanding catalytic performance under mild reaction conditions.

7.
PLoS One ; 15(7): e0236911, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32702072

RESUMO

[This corrects the article DOI: 10.1371/journal.pone.0227869.].

8.
Int J Syst Evol Microbiol ; 70(6): 3845-3851, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32501782

RESUMO

A novel endophytic actinomycete, designated strain p1410T, was isolated from the root of cattail pollen (Typha angustifolia L.) and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus Nonomuraea. It produced spiral chains of spores on aerial mycelium as well as forming a pseudosporangium. Whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, ribose and madurose. The menaquinones detected were MK-9(H2), MK-9(H4) and MK-9(H0). The major fatty acids were 10-methyl C17 : 0, iso-C16 : 0 and C17 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol mannoside and an unknown glycolipid. The DNA G+C content of the draft genome sequence, consisting of 11.4 Mbp, was 70.9 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed that strain p1410T belongs to the genus Nonomuraea with the highest sequence similarity to Nonomuraea candida HMC10T (98.6 %), but phylogenetically clustered with Nonomuraea endophytica YIM 65601T (98.4 %) and Nonomuraea longicatena NRRL 15532T (98.3 %). Based on its phenotypic characteristics, DNA-DNA relatedness and average nucleotide identity, the strain is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea typhae sp. nov. is proposed. The type strain is p1410T (=CCTCC AA 2019044T=JCM 33461T).


Assuntos
Actinobacteria/classificação , Filogenia , Raízes de Plantas/microbiologia , Typhaceae/microbiologia , Actinobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
9.
Dalton Trans ; 49(26): 8933-8948, 2020 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-32558836

RESUMO

The first polyhydroxycarboxylic acid ligand bridged multi-EuIII-incorporated tellurotungstate K14H10[Eu4(H2O)4W6(H2glu)4O12(B-α-TeW9O33)4]·60H2O (H6glu = d-gluconic acid) (1) was synthesized via an organic ligand-driven self-assembly strategy. The polyhydroxycarboxylic acid ligand bridged tetrameric polyoxoanion [Eu4(H2O)4W6(H2glu)4O12(B-α-TeW9O33)4]24- in 1 can be viewed as an aggregation of four trivacant Keggin [B-α-TeW9O33]8- fragments and an innovative heterometallic [Eu4(H2O)4W6(H2glu)4O12]8+ cluster, in which four high-coordinate polyhydroxy flexible H2glu4- ligands chelate W and Eu centers through carboxyl and hydroxyl groups, giving rise to a heterometallic cluster. The hexagonal packing of the tetrameric polyoxoanions in 1 along the c axis provides excellent porous channels, which greatly increases the specific surface area of the whole framework and may be of benefit for fluorescence sensing in aqueous solution. 1 can function as a "turn-off" luminescence sensor to detect Cu2+ ions in aqueous solution. The limit of detection (LOD) of the 1-sensor is 8.82 × 10-6 mM, which is the lowest among the reported polyoxometalate-based fluorescence sensors. As for the Cu2+-quenching system, it can function as an "off-on" sensor to detect cysteine in an aqueous system, affording a LOD of 1.75 × 10-4 mM. This work opens up an avenue to broaden the applications of polyoxometalate-based materials in the optical intelligence detection field.

10.
Int J Infect Dis ; 97: 69-77, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32474202

RESUMO

OBJECTIVES: This study aimed to explore the relationship between glycosylated hemoglobin (HbA1c) and the risk of anti-tuberculosis (TB) drug resistance for TB-type 2 diabetes mellitus (T2DM) patients. METHODS: From March 2014 to June 2019, medical records from multiple centers were searched. Logistic regression analyses were performed. A predictive model for multidrug-resistance (MDR) was developed and validated. Calibration and discrimination of the model were assessed. RESULTS: Inconsistent results were found in the systemic review. A multicenter chart review with 657 records was thus conducted. The HbA1c <7% group and HbA1c ≥7% group had 390 and 267 patients, respectively. The HbA1c<7% group had a lower risk of developing rifampicin resistance, isoniazid resistance and MDR, with odd ratios (ORs) of 1.904 (p=0.001), 2.896 (p<0.001) and 3.228 (p<0.001), respectively. The between-group differences in the risk of anti-TB drug resistance were analyzed based on data from three provinces in China. After adding HbA1c grading, the predictive model for MDR (https://mengyuan.shinyapps.io/Shinyapp/) showed excellent capacity with an AUC of 75.4% in the training set (Sichuan and Gansu) and 73.9% in the internal validation set (Henan). The performances in calibration, prediction probabilities and net clinical benefit were significantly improved by HbA1c grading. CONCLUSIONS: HbA1c grading was an independent risk factor for isoniazid resistance and MDR in TB-T2DM patients.


Assuntos
Antituberculosos/uso terapêutico , Diabetes Mellitus Tipo 2/complicações , Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico , Adulto , Idoso , Farmacorresistência Bacteriana , Farmacorresistência Bacteriana Múltipla , Feminino , Hemoglobina A Glicada/análise , Humanos , Isoniazida/uso terapêutico , Masculino , Pessoa de Meia-Idade , Mycobacterium tuberculosis/efeitos dos fármacos , Razão de Chances , Rifampina/uso terapêutico , Fatores de Risco , Tuberculose/tratamento farmacológico
11.
Arch Gynecol Obstet ; 302(2): 455-462, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32504196

RESUMO

PURPOSE: Cervical cancer is still one of the main causes of death in females. Conventional diagnostic tools such as colposcopy are still unsatisfactory, so accurate diagnostic tools for cervical diseases are needed. Therefore, the purpose of this study was to perform a clinical study to evaluate the value of microendoscopic imaging systems in the diagnosis of cervical precancerous lesions and cervical microinvasive carcinoma (MIC). METHODS: Totally 106 patients ranging in age from 23 to 67 years were recruited. All patients had abnormal thin-layer cytology (TCT) results (≥ low-grade squamous intraepithelial lesions) and high-risk human papillomavirus (HPV) positivity. Each patient was first subjected to ordinary colposcopy, followed by microendoscopy and biopsy. All results of the colposcopy and microendoscopy images were compared to the histopathological diagnosis. RESULTS: Characteristics of pathological blood vessels were easily distinguished by microendoscopy compared with ordinary colposcopy. The diagnostic agreement rate of microendoscopy with the pathological diagnosis was higher (95.3%) than that of ordinary colposcopy (37.7%) (weighted kappa = 0.863, P < .01). When diagnosing HSIL and more advanced disease, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of the microendoscopic diagnosis were significantly higher than those of ordinary colposcopy (97.6 and 38.1%), (95.5 and 63.6%), (98.8 and 80.0%), (91.3 and 21.2%) and (97.7 and 43.4%), respectively. CONCLUSION: This study shows that microendoscopy has important value in the diagnosis of cervical lesions which can provide real-time diagnosis in vivo without staining, particularly for lesions that are not sensitive to acetic acid staining.

12.
Nanoscale ; 12(19): 10842-10853, 2020 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-32396585

RESUMO

Under coordination driving force of tungsten and rare-earth (RE) bridges, we synthesized a novel giant multi-tungsten-and-RE-bridging octameric tellurotungstate (TT) [H2N(CH3)2]16K8Na6H10[Pr8(H2O)20W16O48][B-α-TeW9O33]8·70H2O (1) in CH3CN-H2O mixed solvent. The cluster anion {[Pr8(H2O)20W16O48][B-α-TeW9O33]8}40- features sixteen WVI bridges, eight PrIII bridges and eight trivacant Keggin [B-α-TeW9O33]8- fragments, which the square {W4O12} cluster can be imagined as a seed to induce the aggregation of eight [B-α-TeW9O33]8- fragments by coordination driving force of additional twelve WVI bridges and eight PrIII ions. Furthermore, the 2D 1@DODA (dimethyldioctadecyl ammonium bromide = DODA·Br) honeycomb composite material was prepared. The honeycomb morphology of the 1@DODA composite material provides rich binding sites for electrodepositing Au nanoparticles to make Au/1@DODA electrodes. The aptamer of 17ß-estradiol (E2) hormone can be grafted to the Au/1@DODA electrodes via Au-S bonding interaction to construct the Au/1@DODA aptamer biosensors. By virtue of the specific recognition interaction of aptamer and the electrochemical signal amplification function of methylene blue and cDNA, the Au/1@DODA aptamer biosensors can realize the electrochemical detection of E2. This finding not only offers an electrochemical biosensing platform for detecting E2, but also expands POM-based composite materials in the applications of clinical detection and biological analysis.

13.
Inorg Chem ; 59(10): 6839-6848, 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32378410

RESUMO

Developing organic-inorganic hybrid lanthanide(Ln)-functionalized polyoxometalates (POMs) has long been a main research topic. In this article, two novel hexameric Ln-containing arsenotungstates modified by 2,5-thiophenedicarboxylic acid (H2tpdc) [H2N(CH3)2]21Na13{Ln6W14O40(H2O)18(Htpdc)2}[B-α-AsW9O33]6·nH2O [Ln = Eu3+, n = 94 (1Eu); Ln = Gd3+, n = 114 (2Gd)] were successfully obtained, and the sulfur-heterocyclic carboxylic acid ligand was for the first time introduced to the POM system. Both polyoxoanion skeleton structures can be regarded as an aggregation of six trivacant Keggin [B-α-AsW9O33]9- entities linked by a S-shaped [Ln6W14O40(H2O)18(Htpdc)2]20- cluster. More surprisingly, two carboxyl O atoms in Htpdc- groups directly coordinate with two W atoms other than Ln atoms, which is rarely seen in tungsten-oxo clusters. Fluorescence properties of 1Eu in solid state and aqueous solution have been systematically investigated. Also, 1Eu as a fluorescence sensor in aqueous solution toward the detection of the Ba2+ ion has been explored. As expected, 1Eu may be a promising fluorescence probe for the Ba2+ detection in the absence of Ca2+ or Sr2+ ions in aqueous environment with high sensitivity, good selectivity, and low detection limit (1.19 × 10-3 mM). This work not only first introduces the S-heterocyclic carboxylic acid ligand into the POM system and enriches the types of organic ligands which can be used in POM chemistry but also broadens the POMs' application in fluorescence detection.

14.
Int J Syst Evol Microbiol ; 70(5): 3226-3233, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32375929

RESUMO

A novel actinomycete, designated strain NEAU-C151T, was isolated from soil collected from Mount Song and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that strain NEAU-C151T belongs to the genus Streptomyces and exhibited 97.5, 97.4 and 97.4 % similarities to Streptomyces lincolnensis NRRL 2936T, Streptomyces coacervatus AS-0823T, and Streptomyces longisporus ISP 5166T, respectively. The assignment of strain NEAU-C151T to the genus Streptomyces was confirmed by chemotaxonomic data: anteiso-C15 : 0, C16 : 0, iso-C16 : 0, C16 : 1 (ω7c) and anteiso-C17 : 0 as the major cellular fatty acids; whole-cell sugars contained ribose and glucose; phospholipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), unidentified phospholipid (PL), unidentified lipids (L) and phosphatidylinositol mannoside (PIM); the menaquinones were MK-9(H4), MK-9(H6), MK-10(H2) and MK-9(H8). However, multilocus sequence analysis based on five other house-keeping genes (atpD, gyrB, recA, rpoB, and trpB), DNA-DNA relatedness and phenotypic data showed that strain NEAU-C151T could be distinguished from its closest relatives. Consequently, strain NEAU-C151T represents a novel species of the genus Streptomyces, for which the name Streptomyces montanus sp. nov. is proposed. The type strain is NEAU-C151T (=CGMCC 4.7498T=DSM 107808T).


Assuntos
Filogenia , Microbiologia do Solo , Streptomyces/classificação , Actinobacteria/genética , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Genes Bacterianos , Tipagem de Sequências Multilocus , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Streptomyces/isolamento & purificação , Vitamina K 2/análogos & derivados , Vitamina K 2/química
15.
Int J Syst Evol Microbiol ; 70(5): 3300-3308, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32375931

RESUMO

A Gram-stain-positive, non-motile, creamy-white actinobacterium, which has an elementary branching rod-coccus life cycle was isolated from the rhizosphere soil of rice (Oryza sativa L.) collected from Northeast Agricultural University in Harbin, Heilongjiang province, north-east PR China, and its taxonomic status was examined by using a polyphasic approach. Results from the 16S rRNA gene sequence study showed that the isolate, designated strain NEAU-CX67T, belonged to the genus Rhodococcus and formed a cluster with Rhodococcus maanshanensis DSM 44675T, Rhodococcus kronopolitis NEAU-ML12T and Rhodococcus tukisamuensis JCM 11308T (98.3, 98.1 and 97.7% gene sequence similarity, respectively). The major fatty acids were C16 : 0, 10-methyl C18 : 0, C18 : 1 ω9c and C16 : 1 ω7c. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major isoprenoid quinone was MK-8(H2). Whole-cell hydrolysates contained meso-diaminopimelic acid. Arabinose, galactose and ribose were detected as diagnostic sugars from whole-cell hydrolysates. Mycolic acids were detected. The genomic DNA G+C content of strain NEAU-CX67T was 64.6 mol%. Strain NEAU-CX67T exhibited low average nucleotide identity and digital DNA-DNA hybridization values with R. maanshanensis DSM 44675T (92.1 and 45.4 %) and R. tukisamuensis JCM 11308T (81.9 and 24.4 %). On the basis of results of phylogenetic, genotypic, physiological and chemotaxonomic analysis, strain NEAU-CX67T is considered to represent a novel species of the genus Rhodococcus for which the name Rhodococcus oryzae sp. nov. is proposed. The type strain is NEAU-CX67T (=DSM 107701T=CCTCC AB 2018233T).


Assuntos
Oryza/microbiologia , Filogenia , Rizosfera , Rhodococcus/classificação , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodococcus/isolamento & purificação , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
16.
Lab Med ; 2020 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-32447387

RESUMO

Rapid and accurate diagnosis of multidrug-resistant tuberculosis (MDR-TB) is important for timely and appropriate therapy. In this study, a rapid and easy-to-perform molecular test that integrated polymerase chain reaction (PCR) amplification and a specific 96-well microplate hybridization assay, called PCR-ELISA (enzyme-linked immunosorbent assay), were developed for detection of mutations in rpoB, katG, and inhA genes responsible for rifampin (RIF) and isoniazid (INH) resistance and prediction of drug susceptibility in Mycobacterium tuberculosis clinical isolates. We evaluated the utility of this method by using 32 multidrug-resistent (MDR) isolates and 22 susceptible isolates; subsequently, we compared the results with data obtained by conventional drug susceptibility testing and DNA sequencing. The sensitivity and specificity of the PCR-ELISA test were 93.7% and 100% for detecting RIF resistance, and 87.5% and 100% for detecting INH resistance, respectively. These results were comparable to those yielded by commercially available molecular tests such as the GenoType MTBDRplus assay. Based on the aforementioned results, we conclude that the PCR-ELISA microplate hybridization assay is a rapid, inexpensive, convenient, and reliable test that will be useful for rapid diagnosis of MDR-TB, for improved clinical care.

17.
Microorganisms ; 8(3)2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-32244993

RESUMO

Cancer is a serious threat to human health. With the increasing resistance to known drugs, it is still urgent to find new drugs or pro-drugs with anti-tumor effects. Natural products produced by microorganisms have played an important role in the history of drug discovery, particularly in the anticancer and anti-infective areas. The plant rhizosphere ecosystem is a rich resource for the discovery of actinomycetes with potential applications in pharmaceutical science, especially Streptomyces. We screened Streptomyces-like strains from the rhizosphere soil of wheat (Triticum aestivum L.) in Hebei province, China, and thirty-nine strains were obtained. Among them, the extracts of 14 isolates inhibited the growth of colon tumor cell line HCT-116. Strain NEAU-wh-3-1 exhibited better inhibitory activity, and its active ingredients were further studied. Then, 16S rRNA gene sequence similarity studies showed that strain NEAU-wh3-1 with high sequence similarities to Embleya scabrispora DSM 41855T (99.65%), Embleya hyalina MB891-A1T (99.45%), and Streptomyces lasii 5H-CA11T (98.62%). Moreover, multilocus sequence analysis based on the five other house-keeping genes (atpD, gyrB, rpoB, recA, and trpB) and polyphasic taxonomic approach comprising chemotaxonomic, phylogenetic, morphological, and physiological characterization indicated that the isolate should be assigned to the genus Embleya and was different from its closely related strains, therefore, it is proposed that strain NEAU-wh3-1 may be classified as representatives of a novel species of the genus Embleya. Furthermore, active substances in the fermentation broth of strain NEAU-wh-3-1 were isolated by bioassay-guided analysis and identified by nuclear magnetic resonance (NMR) and mass spectrometry (MS) analyses. Consequently, one new Zincophorin analogue together with seven known compounds was detected. The new compound showed highest antitumor activity against three human cell lines with the 50% inhibition (IC50) values of 8.8-11.6 µg/mL and good antibacterial activity against four pathogenic bacteria, the other known compounds also exhibit certain biological activity.

18.
J Colloid Interface Sci ; 572: 141-150, 2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-32240787

RESUMO

Harvesting broad spectral absorption and visible light photocatalysis of ultrawide bandgap semiconductors are one of most significative topics in the solar energy conversion and utilization fields. In this work, amorphous Cl-Ta2O5-x microspheres were prepared by facile solvothermal method for stabilized visible light photocatalytic hydrogen generation. The acetone absorbed on the interfaces of Ta2O5 nanoparticles induced the formation of oxygen vacancies, enhanced visible light absorption, and formation of Ta2O5-x microspheres with preferred orientations as well as Cl doping. The Cl-Ta2O5-x microspheres showed typical amorphous characteristics and obvious visible light absorption in comparison to those of commercial Ta2O5. More importantly, the prepared Cl-Ta2O5-x microspheres also showed stabilized visible light photocatalytic hydrogen generation performance in the spectral regions of 400 nm ≤ λ ≤ 600 nm mainly because of the introduction of oxygen vacancy defects and Cl doping, which might significantly expand the application of tantalum oxide semiconductors in the broad spectral photocatalytic water splitting.

19.
Clin Immunol ; 215: 108444, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32339669

RESUMO

Mast cells play a significant role in urticaria pathogenesis. It's evidenced that vitamin D has positive impact in chronic spontaneous urticaria (CSU) recently, but underlying mechanisms remain unclear. In this study, isobaric tag for relative and absolute quantification-liquid chromatography-mass spectrometer/mass spectrometer was used to detect the expression of proteins in sera of CSU patients and healthy subjects. Thirty-one differentially expressed proteins were identified, in which vitamin D binding protein (VDBP) was higher in CSU patients than that in healthy subjects after verification. Our results indicated that sera of CSU patients induced the production of vascular endothelial growth factor (VEGF) in mast cells through PI3K/Akt/p38 MAPK/HIF-1α axis in an IgE-depended way, and 25(OH)D3 suppressed the expression of VEGF by inhibiting this signaling pathway axis in this process. Collectively, these results suggest VDBP to be a potential biomarker and propose a potential mechanism of benefit for vitamin D therapy in CSU.

20.
Plant Dis ; 104(6): 1601-1609, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32320370

RESUMO

Rice is used as a staple food in different areas of world, especially in China. In recent years, rice seedlings have been affected seriously by symptoms resembling bacterial palea browning (BPB) in Heilongjiang Province. To isolate and identify the pathogenic bacteria responsible for the disease, 40 bacterial strains were isolated from diseased rice seedlings collected from the four major accumulative-temperature zones of rice fields cultivated in Heilongjiang Province, and these were identified as 13 species based on morphological characteristics and 16S ribosomal RNA (rRNA) gene sequences. Inoculation of all the isolates on healthy rice seedlings showed that the nine Enterobacter cloacae isolates were the pathogens causing typical symptoms of BPB, including yellowing to pale browning, stunting, withering, drying, and death. Moreover, the nine E. cloacae isolates could also cause symptoms of bacterial disease on the seedlings of soybean (Glycine max), maize (Zea mays L.), and tomato (Solanum lycopersicum). Phylogenetic analysis based on the 16S rRNA gene sequences and phenotypic and biochemical characteristics indicated that these nine pathogenic isolates were E. cloacae. In addition, analysis of the sequences of four housekeeping genes (rpoB, gyrB, infB, and atpD) from the selected strain SD4L also assigned the strain to E. cloacae. Therefore, E. cloacae is the pathogen causing disease of rice seedlings in Heilongjiang Province, which we propose to classify as a form of BPB. To the best of our knowledge, this is the first study to identify E. cloacae as a causal agent of BPB in rice.


Assuntos
Enterobacter cloacae , Oryza , China , Filogenia , RNA Ribossômico 16S , Plântula , Virulência
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA