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1.
Sci Rep ; 13(1): 1388, 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36697471

RESUMO

This case-control study aimed to identify the clinical characteristics and explore the risk factors for liver fibrosis in metabolic associated fatty liver disease (MAFLD) patients with hepatitis B virus (HBV) infection. The patients were grouped into MAFLD + HBV and MAFLD (without HBV infection). Propensity score matching (PSM) was used to match baseline features between the groups. We included 401 patients with biopsy-proven MAFLD, 179 of whom had HBV infection. A total of 83 pairs were successfully matched via PSM, and steatosis scores and ballooning in the MAFLD + HBV group were lower than those in the MAFLD group, while the inflammation scores and liver fibrosis stages were higher. After adjusted for confounding factors, HBV infection was associated with a higher risk of significant liver fibrosis in patients with MAFLD [odds ratio (OR): 3.140, P = 0.003]. Overall, 43.58% (78/179) of patients in the MAFLD + HBV group had significant liver fibrosis. Further multivariate regression analysis, hypertension (OR: 2.640; P = 0.031), type 2 diabetes (OR: 4.939; P = 0.035), and elevated glutamyl-transferase levels (OR: 3.980; P = 0.001) were risk factors for liver fibrosis in the MAFLD + HBV group. This suggests metabolic rather than viral factors are more closely associated with liver fibrosis in MAFLD patients with HBV infection.


Assuntos
Diabetes Mellitus Tipo 2 , Hepatite B , Hepatopatia Gordurosa não Alcoólica , Humanos , Vírus da Hepatite B , Estudos de Casos e Controles , Hepatite B/complicações , Hepatopatia Gordurosa não Alcoólica/complicações , Cirrose Hepática/complicações , Orthohepadnavirus
2.
Environ Int ; 171: 107741, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36628860

RESUMO

BACKGROUND: It remains unclear whether type 2 diabetes and the complication of arthritis are causally related to the PM2.5 pollutant. Therefore, we aimed to investigate the associations of long-term PM2.5 exposure with type 2 diabetes and with arthritis in type 2 diabetes patients. MATERIALS AND METHODS: This study used data from the China Health and Retirement Longitudinal Survey (CHARLS) implemented during 2011-2018. The associations were analyzed by Cox proportional hazards regression models, and the population-attributable fraction (PAF) was calculated to assess the burden of type 2 diabetes and arthritis-attributable to PM2.5. RESULTS: A total of 21,075 participants were finally included, with 19,121 analyzed for PM2.5 and type 2 diabetes risk and 12,427 analyzed for PM2.5 and arthritis risk, of which 1,382 with newly-diagnosed type 2 diabetes and 1,328 with arthritis during the follow-up. Overall, each 10 µg/m3 increment in PM2.5 concentration was significantly associated with an increase in the risk of type 2 diabetes (HR = 1.26, 95 %CI1.22 to 1.31), and the PAF of type 2 diabetes attributable to PM2.5 was 13.54 %. In type 2 diabetes patients, each 10 µg/m3 increment in PM2.5 exposure was associated with an increase in arthritis (HR = 1.42, 95 %CI: 1.28 to 1.57), and the association was significantly greater than that (H = 1.23, 95 %CI: 1.19 to 1.28) in adults without type 2 diabetes. The PAFs of arthritis-attributable to PM2.5 in participants with and without type 2 diabetes were 18.54 % and 10.69 %, respectively. CONCLUSION: Long-term exposure to PM2.5 may increase the risk of type 2 diabetes and make type 2 diabetes patients susceptible to arthritis.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Diabetes Mellitus Tipo 2 , Adulto , Humanos , Diabetes Mellitus Tipo 2/epidemiologia , Diabetes Mellitus Tipo 2/etiologia , Poluentes Atmosféricos/efeitos adversos , Poluentes Atmosféricos/análise , Material Particulado/efeitos adversos , Material Particulado/análise , Exposição Ambiental/análise , China/epidemiologia
3.
Sci Total Environ ; 866: 161386, 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36608829

RESUMO

Municipal sewage sludge has been generated in increasing amounts with the acceleration of urbanization and economic development. The nutrient rich sewage sludge can be recycled by composting that has a great potential to produce stabilized organic fertilizer and substrate for plant cultivation. However, little is known about the metals, pathogens and antibiotic resistance transfer risks involved in applying the composted sludge in agriculture. We studied changes in and relationships between heavy metal contents, microbial communities, and antibiotic resistance genes (ARGs), heavy metal resistance genes (HMRGs) and mobile genetic elements (MGEs) in aerobic composting of sewage sludge. The contents of most of the analyzed heavy metals were not lower after composting. The bacterial α-diversity was lower, and the community composition was different after composting. Firmicutes were enriched, and Proteobacteria and potential pathogens in the genera Arcobacter and Acinetobacter were depleted in the composted sludge. The differences in bacteria were possibly due to the high temperature phase during the composting which was likely to affect temperature-sensitive bacteria. The number of detected ARGs, HMRGs and MGEs was lower, and the relative abundances of several resistance genes were lower after composting. However, the abundance of seven ARGs and six HMRGs remained on the same level after composting. Co-occurrence analysis of bacterial taxa and the genes suggested that the ARGs may spread via horizontal gene transfer during composting. In summary, even though aerobic composting is effective for managing sewage sludge and to decrease the relative abundance of potential pathogens, ARGs and HMRGs, it might include a potential risk for the dissemination of ARGs in the environment.

4.
Artigo em Inglês | MEDLINE | ID: mdl-36674331

RESUMO

Manufacturing agglomeration (MA) is an important way to achieve high-quality industrial development and promote land use efficiency in China. However, there is a lack of research on the relationship between MA and the green use efficiency of industrial land (GUEIL). Based on the panel data of 279 prefecture-level cities in China, from 2004 to 2019, this study analyzes the spatial and temporal differentiation characteristics of MA and GUEIL, then empirically analyzes the impact of MA on GUEIL. The results show that: (1) during the study period, the national MA levels showed a slight decline, followed by a small increase. In addition, the inter-regional differences are mainly characterized as eastern region > central region > northeast region > western region. (2) The national and regional GUEIL showed a trend of increasing, slightly decreasing, and then increasing again. The overall regional differences in efficiency show the characteristics of: eastern region > central region > western region > northeastern region. (3) At the national scale, MA had a "U-shaped" relationship with GUEIL; at the regional scale, MA had a significant "U-shaped" effect on GUEIL in the northeast, central and western regions, while having a single negative effect in the eastern region. Finally, this study provides suggestions for optimizing the manufacturing structure and improving the GUEIL.


Assuntos
Comércio , Indústrias , Cidades , China , Eficiência , Desenvolvimento Econômico
5.
Food Chem ; 410: 135441, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36652799

RESUMO

The effects of superfine yak bone powder (YBP) on human gut microbiota (HGM) were investigated by in vitro digestion and fermentation combined with microbiomics and metabolomics. Results showed that size reduction and protein structural degradation during digestion allowed superfine YBP to release more Ca2+ than CaCO3 powders with similar particle size. Moreover, the indigestible YBP further influenced HGM and was associated with increased occurrence of beneficial bacteria such as Megasphaera spp., Megamonas spp., Acidaminococcus spp., and Prevotella spp. The altered HGM was associated with greater production of short-chain fatty acids with 4-6 carbon atoms. Furthermore, the indigestible YBP was associated with up-regulation of many lipid-related metabolites, including taurine, secondary bile acids, saturated long-chain fatty acids, and ω-3/ω-6 polyunsaturated fatty acids, which modulated favorably lipid metabolic pathways. These findings implied the potential activity of superfine YBP as a food fortifier in favorably altering HGM community structure and regulating lipid metabolism.

6.
Chem Commun (Camb) ; 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36629537

RESUMO

A novel protocol for the synthesis of highly functionalized benzo[b][1,5]diazocin-6(5H)-one derivatives (BDCOs, 4 and 5) from 2-aryl-1H-indoles and 1,1-enediamines was developed via a complex cascade of reactions including regioselective free radical oxidation, the 1,2-addition of imine, imine-enamine tautomerization, intramolecular cyclization, and ring expansion. The cascade reaction was enabled by refluxing a mixture of two substrates in the presence of di-tert-butyl peroxide (DTBP) as an oxidant and anhydrous CuI as a catalyst in toluene under argon protection. Consequently, a series of BDCOs (4 and 5) were synthesized with high regioselectivity in good yield. This protocol can be used for the synthesis of functionalized BDCOs via a one-pot oxidative annulation reaction rather than a multi-step reaction, which is suitable for both combinatorial and parallel syntheses of BDCOs.

7.
Chem Commun (Camb) ; 59(5): 587-590, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36524690

RESUMO

Staphylococcus aureus uses small peptides to assess its population densisty (i.e., quorum sensing) and regulate virulence at high cell number. Here, we report the design and synthesis of peptidomimetics based on these native signals that strongly block this communication pathway in all four specificity groups of S. aureus.


Assuntos
Peptidomiméticos , Infecções Estafilocócicas , Humanos , Staphylococcus aureus/fisiologia , Peptidomiméticos/farmacologia , Peptidomiméticos/metabolismo , Percepção de Quorum , Proteínas de Bactérias/metabolismo , Peptídeos/metabolismo
8.
Medicine (Baltimore) ; 101(47): e31707, 2022 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-36451388

RESUMO

To explore the risk factors for in-stent restenosis (ISR) after stent implantation in patients with coronary heart disease (CHD) using logistic regression analysis. From February 2020 to February 2022, 350 patients with CHD after percutaneous coronary intervention (PCI) were divided into a stent stenosis group and a stent nonstenosis group based on coronary angiography results performed 2 years after PCI. Univariate and multivariate logistic regressions were used to analyze the factors related to ISR after coronary stent implantation in patients with CHD. This study was approved by the Ethics Committee of Shandong University of Traditional Chinese Medicine. Patient signed informed consent. Of the 350 patients with CHD, 138 (39.43%) had stent restenosis while 212 did not. Univariate analysis showed that a family history of CHD, history of type 2 diabetes, hypertension, smoking, and drinking, discontinuation of aspirin, use of conventional dose statins, calcified lesions, ≥ 3 implanted stents, stent length ≥ 30 mm, stent diameter < 3 mm, and tandem stent increased the risk of restenosis. The incidence of restenosis was higher in the stent group than that in the nonstent group (P < .05). There were no significant differences in the blood lipid level, left ventricular ejection fraction, clopidogrel/ticagrelor or beta-blocker withdrawal, location of culprit vessels, and thrombotic lesions between the 2 groups (P > .05). Multivariate logistic regression analysis showed that family history of CHD, history of type 2 diabetes, hypertension, smoking, and drinking, aspirin withdrawal, use of conventional doses of statins, calcified lesions, ≥ 3 implanted stents, stent length ≥ 30 mm, stent diameter < 3 mm, and tandem stenting were risk factors for ISR within 2 years after PCI. A family history of CHD, history of type 2 diabetes, hypertension, smoking, and drinking, discontinuation of aspirin, use of conventional dose statins, calcified lesions, ≥ 3 stent implantations, stent length ≥ 30 mm, stent diameter < 3 mm, and tandem stenting are risk factors for ISR within 2 years after PCI in patients with CHD.


Assuntos
Doença da Artéria Coronariana , Reestenose Coronária , Diabetes Mellitus Tipo 2 , Inibidores de Hidroximetilglutaril-CoA Redutases , Hipertensão , Intervenção Coronária Percutânea , Humanos , Doença da Artéria Coronariana/cirurgia , Constrição Patológica , Intervenção Coronária Percutânea/efeitos adversos , Diabetes Mellitus Tipo 2/complicações , Volume Sistólico , Reestenose Coronária/epidemiologia , Reestenose Coronária/etiologia , Função Ventricular Esquerda , Stents/efeitos adversos , Fatores de Risco , Aspirina/uso terapêutico
9.
Front Microbiol ; 13: 1041146, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36466667

RESUMO

Prodigiosin is a promising secondary metabolite mainly produced by Serratia marcescens. The production of prodigiosin by S. marcescens is regulated by different kinds of regulatory systems, including the EnvZ/OmpR system. In this study, we demonstrated that the regulatory factor OmpR positively regulated prodigiosin production in S. marcescens FZSF02 by directly binding to the promoter region of the prodigiosin biosynthesis cluster with a lacZ reporter assay and electrophoretic mobility shift assay (EMSA). The binding sequence with the pig promoter was identified by a DNase I footprinting assay. We further demonstrate that OmpR regulates its own expression by directly binding to the promoter region of envZ/ompR. For the first time, the regulatory mechanism of prodigiosin production by the transcriptional factor OmpR was revealed.

10.
PLoS One ; 17(12): e0277076, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36469514

RESUMO

BACKGROUND: It is difficult to make a precise diagnosis to distinguish patients with Major Depressive Disorder (MDD) from patients with Bipolar Depressive Disorder (current depressive episode, BD). This study will explore the difference in time perception between MDD and BD using a temporal bisection task. METHODS: In this temporal bisection task, 30 MDD patients, 30 BD patients, and 30 healthy controls (HC) had to categorize a signal duration, between 400 and 1600 milliseconds (ms), as either short or long. A repeated measurement analysis of variance with 3 (subject type) × 7 (time interval) was performed on the long response ratio with Bonferroni correction for multiple comparisons. Origin software was used to calculate the subjective bisection point (BP), difference limen (DL), and Weber ratio (WR). The Hamilton Depression Rating Scale for depression-17 was used to assess depressive symptoms in the patients. RESULTS: The data showed that the interaction effect between subject type and duration was significant (F (6,498) = 4.656, p <0.001, η2p = 0.101). At 400 ms, and the long response of the MDD group was greater than HC group (p<0.017, Bonferroni-corrected). At 1200, 1400 and 1600 ms, the long response of BD group is smaller than HC group, (p<0.017, Bonferroni-corrected). The one-way ANOVA revealed significant difference among the HC, MDD and BD groups in the BP values WR values, F(2, 81) = 3.462, p = 0.036 vs. F(2, 81) = 3.311, p = 0.042. Post-hoc tests showed that the value of BP in the MDD group was less than BD group (p = 0.027) and the value of BP in the MDD group was less than HC group (p = 0.027), while there was not significant difference of BP values between BD group and HC group. The WR values in MDD group larger than the HC group (p = 0.022). LIMITATIONS: Severity of depression not divided and analyzed according to the Hamilton Depression Rating Scale score. CONCLUSION: The time perception of the MDD and BD groups was different from that of the HC group, they overestimated short time periods. Compared with the BD group, the MDD group had a smaller time bisector, and these patients felt that time passed more slowly. The time sensitivity of MDD group and BD group were less than the HC group. However, there was no statistical difference in time sensitivity between the MDD and BD groups.


Assuntos
Transtorno Bipolar , Transtorno Depressivo Maior , Percepção do Tempo , Humanos , Transtorno Bipolar/diagnóstico , Transtorno Depressivo Maior/diagnóstico , Emoções
11.
RSC Adv ; 12(52): 33852-33858, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36505719

RESUMO

Metal-organic framework (MOF) material is one of the most promising porous nanomaterials for volatile organic compound (VOC) adsorption and sensing. The large surface area and the high porosity of MOF contribute to the high sensitivity of MOF-based VOC sensors. In this study, we engineer the coating of the zeolitic imidazolate framework material ZIF-8 grown on the surface of a long-period fiber grating (LPFG) for acetone vapor sensing. Being a periodic structure formed in a single-mode optical fiber, an LPFG is designed to couple light from the core to the cladding of the fiber at a specific resonance wavelength. Adsorption of acetone vapor molecules in the framework of the ZIF-8 coating can change the refractive index of the coating and cause a shift in the resonance wavelength of the LPFG. The sensitivity of the resonance shift of the LPFG to the acetone vapor concentration depends strongly on the thickness of the ZIF-8 coating. To create a dense ZIF-8 coating, at least five growth cycles of ZIF-8 (30 min growth for one cycle) are required, and nine growth cycles can create a 500 nm thick coating. The LPFG coated with nine growth cycles of ZIF-8 provides a high sensitivity of 21.9 nm ppm-1, a low detection limit of 1.4 ppm, and a wide detection range of about 1500 ppm. Our results can facilitate the development of high-performance optical fiber sensors based on MOF for VOC detection.

12.
iScience ; 25(12): 105605, 2022 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-36505920

RESUMO

A high abundance of tumor-infiltrating lymphocytes (TILs) has a positive impact on the prognosis of patients with lung adenocarcinoma (LUAD). We aimed to develop and validate an artificial intelligence-driven pathological scoring system for assessing TILs on H&E-stained whole-slide images of LUAD. Deep learning-based methods were applied to calculate the densities of lymphocytes in cancer epithelium (DLCE) and cancer stroma (DLCS), and a risk score (WELL score) was built through linear weighting of DLCE and DLCS. Association between WELL score and patient outcome was explored in 793 patients with stage I-III LUAD in four cohorts. WELL score was an independent prognostic factor for overall survival and disease-free survival in the discovery cohort and validation cohorts. The prognostic prediction model-integrated WELL score demonstrated better discrimination performance than the clinicopathologic model in the four cohorts. This artificial intelligence-based workflow and scoring system could promote risk stratification for patients with resectable LUAD.

13.
Mamm Genome ; 2022 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-36550326

RESUMO

In 2001, three independent groups reported the identification of a novel cluster of progenitor cells that contribute to heart development in mouse and chicken embryos. This population of progenitor cells was designated as the second heart field (SHF), and a new research direction in heart development was launched. Twenty years have since passed and a comprehensive understanding of the SHF has been achieved. This review provides retrospective insights in to the contribution, the signaling regulatory networks and the epithelial properties of the SHF. It also includes the spatiotemporal characteristics of SHF development and interactions between the SHF and other types of cells during heart development. Although considerable efforts will be required to investigate the cellular heterogeneity of the SHF, together with its intricate regulatory networks and undefined mechanisms, it is expected that the burgeoning new technology of single-cell sequencing and precise lineage tracing will advance the comprehension of SHF function and its molecular signals. The advances in SHF research will translate to clinical applications and to the treatment of congenital heart diseases, especially conotruncal defects, as well as to regenerative medicine.

14.
Rev Sci Instrum ; 93(12): 124702, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36586935

RESUMO

A reliable and repeatable triggering technology for a megavolt gap switch with a low working coefficient η is an urgent need and a research focus. In this study, a novel method of hybrid plasma injection (HPI) driven by pulsed discharge inside a capillary was first proposed. The HPI actuator adopted a metal-polytetrafluoroethylene (PTFE)-stacked capillary, in which severe ablation could generate a hybrid plasma containing gas and metal vapor ionized component ejected outward from the nozzle. The HPI actuator could perform repeatedly with an extremely strong plasma injection and triggering ability and, thus, provided a solution for megavolt ultrafast bypass switches (UFBPSs). The evolution and the trigger properties of the HPI actuator were investigated, and the influence of the stacked material (Al, Zn, and Sn) and its proportion (3/15, 7/15, and 10/15) was studied, followed by the performance degradation in multi-shot. It was found that stacking chemically active and low-ionization-energy aluminum in a proportion of 7/15 strongly enhanced the HPI, with an initial velocity of 1200 m/s and a maximum height of 7.5 cm in 0.5 MPa SF6. In repeated operations, the HPI actuator performance degraded obviously due to capillary expansion and deformation, and the lifetime was tens of magnitude. Finally, the optimized HPI actuator was used to trigger a 7 cm-0.5 MPa SF6 gap, with a breakdown voltage of ∼1.5 MV. When a 100 kV DC voltage was applied (η < 7%), the gap was successfully and continuously triggered for 27 shots with the trigger delay ranging from 301 to 670 µs, indicating that the HPI actuator could effectively and repeatedly trigger megavolt-magnitude SF6 gaps at a very low η and was a good solution for megavolt UFBPSs.

15.
Artigo em Inglês | MEDLINE | ID: mdl-36513871

RESUMO

Anxiety is a normal and transitory emotional state that allows the organisms to cope well with the real or perceived threats, while excessive or prolonged anxiety is a key characteristic of anxiety disorders. We have recently revealed that prolonged anxiety induced by chronic stress is associated with the circuit-varying dysfunction of basolateral amygdala projection neurons (BLA PNs). However, it is not yet known whether similar mechanisms also emerge for acute stress-induced, short-lasting increase of anxiety. Here, using a mouse model of acute restraint stress (ARS), we found that ARS mice showed increased anxiety-like behavior at 2 h but not 24 h after stress, and this effect was accompanied by a transient increase of the activity of BLA PNs. Specifically, ex vivo patch-clamp recordings revealed that the increased BLA neuronal activity did not differ among the distinct BLA neuronal populations, regardless of their projection targets being the dorsomedial prefrontal cortex (dmPFC) or elsewhere. We further demonstrated that such effects were mainly mediated by the enhanced presynaptic glutamate release in dmPFC-to-BLA synapses but not lateral amygdala-to-BLA ones. Furthermore, while optogenetically weakening the presynaptic glutamate release in dmPFC-to-BLA synapses ameliorated ARS-induced anxiety-like behavior, strengthening the release increased in unstressed mice. Together, these findings suggest that acute stress causes short-lasting increase in anxiety-like behavior by facilitating synaptic transmission from the prefrontal cortex to the amygdala in a circuit-independent fashion.

16.
Artigo em Inglês | MEDLINE | ID: mdl-36554908

RESUMO

To explore how environmental factors affected the plankton structure in the Yitong River, we surveyed the water environmental factors and plankton population in different seasons. The results showed high total nitrogen concentrations in Yitong River throughout the year, while the total phosphorus, water temperature (WT), and chemical oxygen demand in summer were significantly higher than those in other seasons (p < 0.05), and the dissolved oxygen (DO) concentrations and TN/TP ratio were significantly lower (p < 0.01) than those in other seasons. There was no significant seasonal change in other environmental factors. Cyanophyta, Chlorophyta, and Bacillariophyta were the main phytoplankton phylum, while Protozoa and Rotifera were the main zooplankton phylum. The abundance and biomass of zooplankton and phytoplankton in the summer were higher than those in other seasons. Non-Metric Multidimensional scaling methods demonstrated obvious seasonal variation of phytoplankton in summer compared to spring and winter, while the seasonal variation of the zooplankton community was not obvious. The results of the redundancy analysis showed that WT, DO and nitrate nitrogen were the main environmental factors affecting phytoplankton abundance. In contrast to environmental factors, phytoplankton was the main factor driving the seasonal variation of the zooplankton community structure. Cyanophyta were positively correlated with the changes in the plankton community.


Assuntos
Cianobactérias , Plâncton , Animais , Estações do Ano , Rios/química , Monitoramento Ambiental , Fitoplâncton , Zooplâncton , Água/análise , Nitrogênio/análise , China , Fósforo/análise
17.
Ann Hepatol ; : 100892, 2022 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-36577468

RESUMO

INTRODUCTION AND OBJECTIVES: The optimal blood pressure (BP) range for patients with metabolic dysfunction-associated fatty liver disease (MAFLD) is currently unknown. This study aimed to explore the relationship between stratified BP levels and MAFLD progression. PATIENTS AND METHODS: The data of adults who underwent yearly health check-ups were screened to establish both a cross-sectional and a 6-year longitudinal cohort of individuals with MAFLD. BP was classified into the following categories optimal, normal, high-normal, and hypertension. Liver fibrosis was diagnosed with fibrosis-4 (FIB-4) score, nonalcoholic fatty liver disease fibrosis score (NFS), and aspartate aminotransferase-to-platelet ratio index (APRI). RESULTS: A total of 10,232 individuals were included in the cross-sectional cohort. In the MAFLD population, individuals with liver fibrosis had significantly higher BP levels and hypertension prevalence (P < 0.001) than those without. Furthermore, liver fibrosis score was significantly associated with BP levels (P < 0.001). In the 6-year longitudinal cohort of 3661 individuals with MAFLD without liver fibrosis, the incidence rates of liver fibrosis increased with increasing BP levels as follows optimal=11.20%, normal=13.9%, high-normal=19.5%, hypertension=26.20% (log-rank 22.205; P < 0.001). Cox regression analysis showed that both baseline high-normal BP (hazard ratio [HR], 1.820; P=0.019) and hypertension (HR, 2.656; P < 0.001) were predictive of liver fibrosis. CONCLUSIONS: BP stratification may be useful in predicting the progression of MAFLD. Individuals having MAFLD with concurrent hypertension or high-normal BP are at a higher risk of liver fibrosis. These findings may provide a criteria for early intervention of MAFLD to prevent liver fibrosis.

18.
Vet Sci ; 9(12)2022 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-36548822

RESUMO

Studying microRNA (miRNAs) in certain agri-food products is attractive because (1) they have potential as biomarkers that may allow traceability and authentication of such products; and (2) they may reveal insights into the products' functional potential. The present study evaluated differences in miRNAs levels in fat and cellular fractions of tank milk collected from commercial farms which employ extensive or intensive dairy production systems. We first sequenced miRNAs in three milk samples from each production system, and then validated miRNAs whose levels in the cellular and fat fraction differed significantly between the two production systems. To accomplish this, we used quantitative PCR with both fractions of tank milk samples from another 20 commercial farms. Differences in miRNAs were identified in fat fractions: overall levels of miRNAs, and, specifically, the levels of bta-mir-215, were higher in intensive systems than in extensive systems. Bovine mRNA targets for bta-miR-215 and their pathway analysis were performed. While the causes of these miRNAs differences remain to be elucidated, our results suggest that the type of production system could affect miRNAs levels and potential functionality of agri-food products of animal origin.

19.
Expert Rev Anticancer Ther ; : 1-7, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36373901

RESUMO

OBJECTIVES: The present study explores an extremely rare disease, thymic mucosa-associated lymphoid tissue (MALT) lymphoma, for its characteristics and prognostic factors by analyzing the Surveillance, Epidemiology, and End Results (SEER) database. METHODS: From 2000 to 2018, cases with a diagnosed thymic MALT lymphoma were extracted. Clinical characteristics, treatments, and survival patterns of these cases were analyzed. RESULTS: Thymic MALT lymphoma (n = 26) accounted for 0.09% of all MALT lymphomas. With a sex ratio of 0.53 (male/female), 68% white population was affected. Most cases were diagnosed with Ann Arbor stage I (50%), yet advanced-stage did not show worse prognosis (p = 0.236). Different treatment protocols did not influence the overall prognosis (p > 0.99). The 5- and 10- year overall survival rates were 83.1% and 78.2%, respectively. Older than 70 years may be an independent risk factor for overall survival (HR = 7.166 [95% CI 1.173-43.756], p = 0.033). CONCLUSION: Thymic MALT lymphoma is a highly rare disease with a favorable prognosis. Ann Arbor staging might not be appropriate to classify severity of this disease or its treatment. Older people may have worse survival. A standardized treatment mode needs to be established, and surgery could remain as the mainstay.

20.
Proc Natl Acad Sci U S A ; 119(48): e2212436119, 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36409903

RESUMO

Riboflavin is produced by most commensal bacteria in the human colon, where enterohemorrhagic Escherichia coli (EHEC) colonizes and causes diseases. Sensing environmental signals to site-specifically express the type-III secretion system (T3SS), which injects effectors into host cells leading to intestinal colonization and disease, is key to the pathogenesis of EHEC. Here, we reveal that EHEC O157:H7, a dominant EHEC serotype frequently associated with severe diseases, acquired a previously uncharacterized two-component regulatory system rbfSR, which senses microbiota-produced riboflavin to directly activate the expression of LEE genes encoding the T3SS in the colon. rbfSR is present in O157:H7 and O145:H28 but absent from other EHEC serotypes. The binding site of RbfR through which it regulates LEE gene expression was identified and is conserved in all EHEC serotypes and Citrobacter rodentium, a surrogate for EHEC in mice. Introducing rbfSR into C. rodentium enabled bacteria to sense microbiota-produced riboflavin in the mouse colon to increase the expression of LEE genes, causing increased disease severity in mice. Phylogenic analysis showed that the O55:H7 ancestor of O157:H7 obtained rbfSR which has been kept in O157:H7 since then. Thus, acquiring rbfSR represents an essential step in the evolution of the highly pathogenic O157:H7. The expression of LEE genes and cell attachment ability of other EHEC serotypes in the presence of riboflavin significantly increased when rbfSR was introduced into them, indicating that those serotypes are ready to use RbfSR to increase their pathogenicity. This may present a potential public health issue as horizontal gene transfer is frequent in enteric bacteria.


Assuntos
Escherichia coli Êntero-Hemorrágica , Escherichia coli O157 , Microbiota , Animais , Humanos , Camundongos , Escherichia coli Êntero-Hemorrágica/genética , Escherichia coli O157/genética , Riboflavina , Virulência/genética , Intestinos
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